CN105494424B - A kind of wheat cold-resistant derivant and its application process - Google Patents

A kind of wheat cold-resistant derivant and its application process Download PDF

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Publication number
CN105494424B
CN105494424B CN201510997562.2A CN201510997562A CN105494424B CN 105494424 B CN105494424 B CN 105494424B CN 201510997562 A CN201510997562 A CN 201510997562A CN 105494424 B CN105494424 B CN 105494424B
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wheat
cold
resistant derivant
resistant
derivant
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CN105494424A (en
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方保停
李向东
邵运辉
王汉芳
岳俊芹
张德奇
秦峰
吕凤荣
杨程
马富举
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Wheat Research Institute Henan Academy Of Agricultural Sciences
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Wheat Research Institute Henan Academy Of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Cultivation Of Plants (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a kind of wheat cold-resistant derivant and its application process, mixed solution that the wheat cold-resistant derivant is made up of hydrogen peroxide, salicylic acid, tween and water, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 80mg 200mg, salicylic acid 200mg 350mg, Tween 80 mg 150mg, surplus is water.The application process of the wheat cold-resistant derivant is:Before low temperature arriving in 12 24h, the blade of wheat is sprayed with wheat cold-resistant derivant, every mu of amount of application is 25 30kg, wherein, the low temperature refers to that environment temperature is less than or equal to 0 DEG C.The wheat cold-resistant derivant low-temperature resistance effect is good, can strengthen wheat low-temperature resistance stress ability, reduce harm of the low temperature to wheat growth, improve wheat yield, and safety and environmental protection, noresidue are environmentally safe to wheat growth without poisoning.

Description

A kind of wheat cold-resistant derivant and its application process
Technical field
The invention belongs to wheat cultivation technical field, and in particular to a kind of wheat cold-resistant derivant and its application process.
Background technology
Winter wheat is China's staple food crop, and its breeding time is long, experience spring and summer four seasons autumn and winter, its turn green the jointing stage easily by To the influence of the Low Temperature in Spring such as cold spell in later spring, late frost stress, Low Temperature in Spring harm has turned into the Major Natural calamity of China's Wheat Production Evil, has a strong impact on wheat yield, threatens grain security.In recent decades, the probability of happening of China's Frost Damage of Winter Wheat is in bright Aobvious ascendant trend, it has also become some areas restrict one of major obstacle factor of Wheat Production, therefore study defence frost damage etc. Low temperature freezing-disaster Hazard Technology, cold tolerance is improved, to ensureing that improving yield of wheat stable yields is significant.Current published application Cold-resistant conditioning agent (such as number of patent application is 201410290669.9,201410290668.4) composition in Wheat Production is multiple It is miscellaneous, preparation method is cumbersome, production cost is high.It is small that the application have studied that a kind of composition is simple, cost is cheap, low-temperature resistance effect is good Wheat cold-resistant derivant, the production development of the application of the product for wheat have important economic implications.
Hydrogen peroxide (also known as hydrogen peroxide, Hydrogen peroxide, H2O2) it is caused by biological cell metabolic process A kind of active oxygen, SCAVENGING SYSTEM OF ACTIVATED OXYGEN can be such that its content is maintained under injury level in plant under normal circumstances, but It is under environment stress, the yield of active oxygen can exceed system Scavenging activity, thereby result in oxidative damage.Past very long one The section time, people are by H2O2It is considered as a kind of harmful products of cellular metabolism, however, increasing research has shown that, plant can profit Use H2O2And amplified and be used as signaling molecule, and then regulate and control growth and development process and various biological and abiotic stimulation is answered Answer.Various biologies and abiotic stress can on certain level H in inducing cell2O2Generation, and then regulate and control a series of stress The signal transduction of response.Research shows that hydrogen peroxide, salicylic acid etc. participate in the regulation of many abiotic stresses, improves a variety of non-lifes The resistance of thing stress.To reduce the harm of low temperature cold damage, wheat yield is improved, using the cold-resistant derivant of certain material, will be had Beneficial to the advantage of increase resistance of wheat derivant, strengthen resistance of wheat.
The content of the invention
It is an object of the invention to provide the wheat cold-resistant derivant that a kind of composition is simple, cost is cheap, low-temperature resistance effect is good, The wheat cold-resistant derivant can strengthen the winter resistance of wheat, improve wheat anti-seismic design, reduce chilling damage, reduce low temperature damage Lose, improve wheat yield.
The technical solution adopted by the present invention is:
A kind of wheat cold-resistant derivant, it is characterised in that the wheat cold-resistant derivant is by hydrogen peroxide, salicylic acid, told The mixed solution of gentle water composition, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 80mg-200mg, bigcatkin willow Sour 200mg-350mg, Tween 80 mg-150mg, surplus are water.
According to above-mentioned wheat cold-resistant derivant, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 100mg, salicylic acid 300mg, tween 100mg, surplus are water.
The application process of above-mentioned wheat cold-resistant derivant is:Before low temperature arriving in 12-24h, with wheat cold-resistant derivant The blade of wheat is sprayed, every mu of amount of application is 25-30kg, wherein, the low temperature refers to that environment temperature is less than or equal to 0 DEG C.
The beneficial effect that the present invention obtains:
(1) wheat cold-resistant derivant low-temperature resistance effect of the invention is good, can strengthen the winter resistance of wheat, and raising wheat resists low Warm stress ability, harm of the low temperature to wheat growth is reduced, reduce low temperature loss, improve wheat yield, meanwhile, of the invention is small Wheat cold-resistant derivant also has booster action to the drought resistance for improving wheat.
(2) wheat cold-resistant derivant composition of the invention is simple, and cost is cheap, using conveniently, can produce on a large scale And popularization and application, there is important economic implications.
(3) wheat cold-resistant derivant safety and environmental protection of the invention, noresidue, and to wheat growth without poisoning, to people and animals Compare safety, it is environmentally safe.
Embodiment
With reference to embodiment, the invention will be further described, but does not limit the scope of the invention.
Embodiment 1:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 100mg, salicylic acid 300mg, tween 100mg, surplus are water.
The preparation method of the wheat cold-resistant derivant is:Weigh 300mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 100mg hydrogen peroxide, 100mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is:Before low temperature arriving in 12-24h, with wheat cold-resistant derivant The blade of wheat is sprayed, every mu of amount of application is 25kg, wherein, the low temperature refers to that environment temperature is less than or equal to 0 DEG C.
Embodiment 2:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 80mg, salicylic acid 350mg, tween 100mg, Surplus is water.
The preparation method of the wheat cold-resistant derivant is:Weigh 350mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 80mg hydrogen peroxide, 100mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is:Before low temperature arriving in 12-24h, with wheat cold-resistant derivant The blade of wheat is sprayed, every mu of amount of application is 30kg, wherein, the low temperature refers to that environment temperature is less than or equal to 0 DEG C.
Embodiment 3:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 120mg, salicylic acid 250mg, tween 120mg, surplus are water.
The preparation method of the wheat cold-resistant derivant is:Weigh 250mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 120mg hydrogen peroxide, 120mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is:Before low temperature arriving in 12-24h, with wheat cold-resistant derivant The blade of wheat is sprayed, every mu of amount of application is 28kg, wherein, the low temperature refers to that environment temperature is less than or equal to 0 DEG C.
Embodiment 4:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 150mg, salicylic acid 200mg, Tween 80 mg, Surplus is water.
The preparation method of the wheat cold-resistant derivant is:Weigh 200mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 150mg hydrogen peroxide, 80mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is:Before low temperature arriving in 12-24h, with wheat cold-resistant derivant The blade of wheat is sprayed, every mu of amount of application is 26kg, wherein, the low temperature refers to that environment temperature is less than or equal to 0 DEG C.
Embodiment 5:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 180mg, salicylic acid 280mg, tween 150mg, surplus are water.
The preparation method of the wheat cold-resistant derivant is:Weigh 280mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 180mg hydrogen peroxide, 150mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is:Before low temperature arriving in 12-24h, with wheat cold-resistant derivant The blade of wheat is sprayed, every mu of amount of application is 29kg, wherein, the low temperature refers to that environment temperature is less than or equal to 0 DEG C.
Embodiment 6:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 200mg, salicylic acid 200mg, tween 140mg, surplus are water.
The preparation method of the wheat cold-resistant derivant is:Weigh 200mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 200mg hydrogen peroxide, 140mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is same as Example 1.
Embodiment 7:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 120mg, salicylic acid 300mg, tween 120mg, surplus are water.
The preparation method of the wheat cold-resistant derivant is:Weigh 300mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 120mg hydrogen peroxide, 120mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is same as Example 2.
Embodiment 8:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 160mg, salicylic acid 220mg, tween 90mg, Surplus is water.
The preparation method of the wheat cold-resistant derivant is:Weigh 220mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 160mg hydrogen peroxide, 90mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is same as Example 3.
Embodiment 9:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 100mg, salicylic acid 340mg, tween 130mg, surplus are water.
The preparation method of the wheat cold-resistant derivant is:Weigh 340mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 100mg hydrogen peroxide, 130mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is same as Example 3.
Embodiment 10:
A kind of wheat cold-resistant derivant, the wheat cold-resistant derivant are made up of hydrogen peroxide, salicylic acid, tween and water Mixed solution, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 150mg, salicylic acid 320mg, tween 100mg, surplus are water.
The preparation method of the wheat cold-resistant derivant is:Weigh 320mg salicylic acids and be dissolved in 500ml water and water is prepared Poplar acid solution, 150mg hydrogen peroxide, 100mg tweens are added into salicylic acid solution, is uniformly mixed, is then settled to water 1L, wheat cold-resistant derivant was both obtained after stirring.
The application process of the wheat cold-resistant derivant is same as Example 1.
Embodiment 11:
The present embodiment uses the wheat in the jointing stage obtained cold-resistant derivant in 1-10 of the embodiment of the present invention to carry out Sprinkling is handled, influence of the research wheat cold-resistant derivant to wheat leaf blade physical signs.
Specific experiment is:Condition of culture of the selection in the jointing stage is consistent, the uniform field pipe of growing way plants wheat 60 and manages (pipe A diameter of 20cm, pipe range 60cm, often 10 plants of wheats of pipe), it is divided into 12 groups (every group 5 is managed);The pipe of 1-10 groups is planted respectively The wheat cold-resistant derivant that wheat leaf blade sprinkling 1-10 of the embodiment of the present invention is prepared, wheat leaf is planted to the 11st, 12 group of pipe Piece sprays clear water, wherein, the standard of sprinkling is:Blade is set to moisten completely, but liquid does not flow down;By the pipe of 1-11 groups after 12h Plant wheat move into phjytotron carry out low temperature induction processing, low temperature induction processing terminate after by 1-11 groups pipe cultivation wheat again Move to field growing.12nd group of pipe, which planted after wheat leaf blade sprayed clear water to be still grown in the field, (records two days highest temperatures as 23 DEG C, the lowest temperature is 5 DEG C, 12.7 DEG C of temperature on average).After 1-11 groups pipe cultivation wheat moves to field growing 12 hours, take respectively The most upper expansion leaf of 1-12 group wheats plants wheat as control using the 12nd group of pipe, determines 1-12 groups respectively as research object Pipe plants the content of MDA in the most upper expansion leaf blade of wheat, soluble sugar, soluble protein and proline, and it the results are shown in Table 1.
Wherein, the process of the low temperature induction processing is:The temperature of phjytotron is reduced to 0 with 2 DEG C/h speed DEG C, and temperature is maintained into 0 DEG C of 48h, the temperature of phjytotron is then increased to by outdoor temperature with 2 DEG C/h speed.
In this experiment, mda content is determined using thiobarbituricacidα- method side, and soluble sugar content uses anthrone colorimetric Method is determined, and soluble protein content is determined using Coomassie brilliant G-250 method, and proline content is surveyed using acid ninhydrine method It is fixed.
MDA is one of product of Lipid peroxidation metabolism, the size of the big I reaction film degree of injury of its content, therefore The content of MDA is a common counter in plant senescence physiology and Resistance Physiology research.As shown in Table 1, in this experiment The content of MDA significantly raises in 11 groups of wheat leaf blades, and sprays in the 1-10 group wheat leaf blades of cold-resistant derivant the third two The content of aldehyde differs smaller with the 12nd group of wheat, it can be seen that low temperature can be relieved by spraying cold-resistant derivant of the present invention Coerce the injury to wheat leaf blade cell membrane.
Soluble sugar, soluble protein and proline are the osmotic adjustments of Chinese medicine in plant cell, to improving plant Total metabolism it is horizontal, to maintain plant osmosis regulating power to have the function that important, under the conditions of low temperature stress, the 11st group in this experiment The content of soluble sugar, soluble protein and proline is relatively low in wheat leaf blade, differs larger with the 12nd group;And sprayed cold-resistant The content of soluble sugar, soluble protein and proline is higher in the 1-10 group wheat leaf blades of derivant, with the 12nd group of wheat Difference is smaller, it is possible thereby to illustrate that soluble sugar in wheat leaf blade, soluble protein can be improved by spraying cold-resistant derivant of the present invention With the content of proline, the metaboilic level and osmotic adjustment ability of wheat can be improved to a certain extent, so as to improve itself Resist the influence of extraneous low temperature.
Influence of the wheat cold-resistant conditioning agent of table 1 to wheat seedlings blade indices
Embodiment 12:Influence of the cold-resistant derivant to wheat yield
The present embodiment uses the wheat in the jointing stage obtained cold-resistant derivant in 1-10 of the embodiment of the present invention to carry out Sprinkling is handled, influence of the research wheat cold-resistant derivant to wheat yield.
Specific experiment is:Condition of culture of the selection in the jointing stage is consistent, the uniform field pipe of growing way plants wheat 60 and manages (pipe A diameter of 20cm, pipe range 60cm, often 10 plants of wheats of pipe), it is divided into 12 groups (every group 5 is managed);The pipe of 1-10 groups is planted respectively The wheat cold-resistant derivant that wheat leaf blade sprinkling 1-10 of the embodiment of the present invention is prepared, wheat leaf is planted to the 11st, 12 group of pipe Piece sprays clear water, wherein, the standard of sprinkling is:Blade is set to moisten completely, but liquid does not flow down;By the pipe of 1-11 groups after 12h Plant wheat move into phjytotron carry out low temperature induction processing, low temperature induction processing terminate after by 1-11 groups pipe cultivation wheat again Move to field growing.12nd group of pipe plants wheat leaf blade and sprayed the latter disposed upright of clear water in field growing.Treat that 1-12 groups wheat is given birth to It is long to after the maturity period, investigating 1-12 groups pipe cultivation wheat single-strain tassel number, grain number per spike, mass of 1000 kernel and the every yield of pipe wheat respectively, Using the 12nd group as control, count different cold-resistant derivants influences on wheat yield and its constituent element, and it the results are shown in Table 2.
Wherein, the process of the low temperature induction processing is:The temperature of phjytotron is reduced to 0 with 2 DEG C/h speed DEG C, and temperature is maintained into 0 DEG C of 48h, the temperature of phjytotron is then increased to by outdoor temperature with 2 DEG C/h speed.
As shown in Table 2, do not spray cold-resistant derivant low-temperature treatment the 11st group of pipe plant the single-strain tassel number of wheat, grain number per spike, The 12nd group of mass of 1000 kernel and often pipe yield more low-temperature treatment reduces by 1.2,14.8,8.9g and 31.3g respectively, and sprays The 1-10 groups of cold-resistant derivant only reduce by 0.4,5.2,5.3g and 15.1g compared with the 12nd group.Sprayed cold-resistant induction The 1-10 groups of agent have certain protective effect compared with the 11st group, it is average increase respectively 0.8,9.7,4.4g and 16.2g.It can be seen that cold-resistant derivant of the present invention mainly increases the yield of wheat by increasing mature spike number and grain number per spike.
Influence of the wheat cold-resistant derivant of table 2 to wheat yield

Claims (3)

1. a kind of wheat cold-resistant derivant, it is characterised in that the wheat cold-resistant derivant is by hydrogen peroxide, salicylic acid, tween With the mixed solution of water composition, the content of each component is in every liter of wheat cold-resistant derivant:Hydrogen peroxide 80mg-200mg, salicylic acid 200mg-350mg, Tween 80 mg-150mg, surplus are water.
2. wheat cold-resistant derivant according to claim 1, it is characterised in that each component in every liter of wheat cold-resistant derivant Content be:Hydrogen peroxide 100mg, salicylic acid 300mg, tween 100mg, surplus are water.
A kind of 3. application process of the wheat cold-resistant derivant described in claim 1, it is characterised in that the 12- before low temperature arriving In 24h, the blade of wheat is sprayed with wheat cold-resistant derivant, every mu of amount of application is 25-30kg, wherein, the low temperature refers to Environment temperature is less than or equal to 0 DEG C.
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CN107232200A (en) * 2017-05-12 2017-10-10 河南农业大学 Defend the composite regulator and method of wheat Spring cold injury
CN107242250A (en) * 2017-06-16 2017-10-13 淮北师范大学 A kind of wheat cold-resistant derivant and its application process
CN111011394B (en) * 2019-12-12 2021-02-02 山东省农作物种质资源中心 Wheat cold-resistant inducer and application method thereof

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