CN104396547B - Method for improving chilling resistance of plant via glycine betaine - Google Patents
Method for improving chilling resistance of plant via glycine betaine Download PDFInfo
- Publication number
- CN104396547B CN104396547B CN201410763618.3A CN201410763618A CN104396547B CN 104396547 B CN104396547 B CN 104396547B CN 201410763618 A CN201410763618 A CN 201410763618A CN 104396547 B CN104396547 B CN 104396547B
- Authority
- CN
- China
- Prior art keywords
- seed
- plant
- glycine betaine
- root
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
Abstract
The invention discloses a method for improving chilling resistance of plant via glycine betaine, and belongs to the technical field of pesticides. The method for improving the chilling resistance of the plant via the glycine betaine has the advantages that four methods of soaking the seeds with glycine betaine, applying soil to root, spraying to leaf surfaces or soaking the fruit are conducted to process the plant of different types, and thus the chilling resistance of the plant and fruit can be obviously improved; the applicable scope is wide, and no potential risk is caused.
Description
Technical field
The present invention relates to technical field of pesticide, a kind of method utilizing glycine betaine to improve Plant chilling resistance.
Background technology
Temperature is the main limiting factor determining plant less than distribution, is also to affect crops and gardening work under cultivation condition
The principal element of thing yield and quality.Damage to plants caused by sudden drop in temperature and refer to above zero low temperature injury produced to plant.Cause damage to plants caused by sudden drop in temperature general 0～
10℃.Damage to plants caused by sudden drop in temperature the change of a series of physiological metabolisms in causing plant, ultimately result in that growth of seedling is slim and frahile, plant strain growth is slow,
Wilting yellow, local necrosis, fruit-setting rate is low, and yield reduces and the generation of degradation under quality.
Semen Tritici aestivi is the cereal crops that China is important, is mainly distributed on to the north of north latitude 30 °.The winter can be divided into little according to habit
Wheat and spring wheat, winter wheat is long relative to the trophophase for spring wheat, from the trophophase to poor growth in reproduction period, experience one therebetween
The cold stage that winter is very long.The north Winter Wheat Area account for the whole nation wheatland gross area 59%, this wheat district the north frequent cold air,
Winter, absolute temperature was low, and weather severe cold reduces the geographical distribution region of winter wheat suitable planting, makes agricultural production suffer the biggest
Loss.
In addition, chilling injury in early spring is one of meteorological disaster main in Semen Maydis agricultural production.Spring Maize Area, the north
Often occurring, the underproduction more than 15% when disaster occurs, Semen Maydis quality is also greatly affected.The vegetable crops such as Fructus Cucumidis sativi, Citrullus vulgaris, Fructus Lycopersici esculenti,
Growth early stage is attacked by cold wave, and its chlorenchyma is destroyed, and branch sprouts and is obstructed, and growth retardation or stopping, even plant are dead
Die.
Through the literature search of prior art is found, anti-cryogen has been utilized to improve the technology of crop cold resistance, such as China
License notification number CN102511480B, patent name is: temperature control sustained-release cold-resistant type seed coat agent and cold-resistance agent preparation method.Should
Anti-cryogen described in patent is only used for tobacco seed and processes, it is impossible to take the plant that other processing modes are applied to outside smoke abatement grass.
Separately there is the technology utilizing genetic engineering to improve crop cold resistance, such as Chinese patent Authorization Notice No. CN102115749B, proprietary term
It is referred to as: TT1 gene purposes in improving plant cold resistance.This patent uses transgenic technology, converts host with recombinant vector
Plant cell, screening obtains and converts cell, then conversion cell culture becomes the cold-resistant plant of transgenic.What this technology was produced turns
Gene prod safety the most fully proves, should not be applied in crop production.
Summary of the invention
Present invention aims to the drawback that existing method exists, propose one and utilize glycine betaine to improve cold resistance of plant
Method in property, the present invention is by glycine betaine regulation Premeabilisation of cells pressure, the activity of protection enzyme, thus improves plant to low temperature
Resistivity.
Realize technical scheme as follows:
1) seed soaking, before accelerating germination, configuration concentration is the aqueous solutions of betaine of 1～5kg/1000L, is thrown by garbled seed
Enter in the alkali solution of beet of configuration, liquid level seed to be exceeded 20～25 centimetres, soak seed 3～5 hours, can be according to the thickness of seed coat
Select to add appropriate hot water and shorten seed soaking time, when, after seed imbibition, pulling seed immediately out and carry out accelerating germination.
2) root is executed, at Seedling Stage or the period of duration of plant growing, with the alkali solution of beet that concentration is 0.5～1kg/1000L
Root executes plant, and root executes time 0.5～2 hours, and the concrete time determines according to the seepage of water of soil or substrate, with complete permeable soil
Or substrate, enable plant root to fully absorb and be advisable.
3) foliage-spray, does medicament spray on plants blade face with the aqueous solutions of betaine that concentration is 0.05～40kg/1000L,
Concrete concentration determines according to the thin degree of plant leaf blade, foliage-spray amount with cover whole blade faces to have droplet drip into
Preferably.
4) fruit soaks, and needs cryopreservation after picking fruit, and configuration concentration is the Radix Betae of 0.5～2 kilogram/1000L
Aqueous slkali, then puts into fruit to soak in solution 3～10 minutes and takes out, get final product cryopreservation after drying.
Described glycine betaine is in the application improved on Plant chilling resistance, it is characterised in that the present invention is directed to different plant, sweet
The application process of dish alkali and consumption are different, and table 1 illustrates application process and the consumption of part common industrial crops glycine betaine.
The mode of the different plant betaine administration of table 1 and concentration
The beneficial effects of the present invention is:
(1) seed treatment before the present invention is applicable to the accelerating germination of corn, veterinary antibiotics, Chinese crude drug, water and fertilizer management after field planting,
Fruit storage after gathering, applied widely.
(2) soaking seed before the present invention is applied to plant accelerating germination, after field planting, water and fertilizer management and fruit storage after gathering, all can show
Write and improve plant and the fruit resistivity to low temperature, if low temperature stress is serious, can reach to improve cold resistance of plant with multi-pass operation
The purpose of property, and plant will not develop immunity to drugs.
(3) the described glycine betaine of the present invention belongs to non-poisonous material, for plant production and food processing process, hidden without any safety
Suffer from.
Specific implementation method
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1: under low temperature stress, aqueous solutions of betaine seed soaking improves Course of Corn Seed Germination ability
Process 1.: before accelerating germination, by boiled water and cold water half and half to good, take a certain amount of pure glycine betaine configuration aqueous solution, with
1000 liters of meters, glycine betaine consumption 2～3 kilograms, 60 corn seeds chosen are put in aqueous solutions of betaine, liquid level kind to be exceeded
Son 20～25 centimetres, wants to stir with waddy in time simultaneously, makes seed be heated evenly.Water temperature to control at 25～28 degrees Celsius,
Soak seed 3～5 hours under this temperature conditions, when, after seed imbibition, pulling seed out and be equally divided into 2 groups；Process 2. for comparison:
Before accelerating germination, by boiled water and cold water half and half to good, 60 corn seeds that will choose put in warm water, water surface seed to be exceeded 20～
25 centimetres, want to stir with waddy in time simultaneously, make seed be heated evenly.Water temperature to control at 25～28 degrees Celsius, in this temperature
Under the conditions of soak seed 3～5 hours, when, after seed imbibition, pulling seed out and be equally divided into 2 groups.By changing low temperature stress temperature
With the low temperature stress time, respectively 2 are processed totally 4 groups of seeds and carry out germination test, testing program and result and see table 2 below:
Endure cold time, Different hypothermia of table 2 difference processes the germination percentage situation (%) of lower seed
Result of the test shows: two kinds of percentages of seedgermination processed become negative correlation with natural law of enduring cold, identical at natural law of enduring cold
Under the conditions of, under 10 DEG C of low temperature, two kinds of germination percentage no significant differences processing seed, process the germination of 1. seed under 20 DEG C of low temperature
2. rate exceeds about 6 times than processing.The cold resistance of corn seed is significantly increased by the seed soaking of garden beet aqueous slkali aqueous solution.
Embodiment 2: under low temperature stress, foliage-spray glycine betaine improves Semen Tritici aestivi vitality
Configuration concentration is the alkali solution of beet of 30kg/1000L, selects 40 strain growing ways identical, and the wheat plant without pest and disease damage divides
Become two groups, be respectively processed 1.: foliage-spray glycine betaine, process 2. comparison: foliage-spray clear water, foliage-spray amount is to cover
All blade face is to there being droplet to drip, and natural air drying absorbs, and after all wheat leaf blade surfaces are all without moisture, puts into 2 DEG C of constant temperature
Low temperature stress 12h in case, measures photosynthesis with the photosynthetic instrument of EGM-4, calculates blade mortality rate.Result of the test see table 3:
The photosynthesis of Semen Tritici aestivi and blade mortality rate situation under table 3 low temperature stress
Result of the test shows: after 2 DEG C of low temperature stress 12h, processes the most compared with the control, and light and total amount improve 57.1%,
Blade mortality rate reduces by 38.5%.Semen Tritici aestivi foliage-spray glycine betaine can effectively alleviate the harm of low temperature stress, improves Semen Tritici aestivi raw
Vigor.
Embodiment 3: under low temperature stress, glycine betaine root is executed cucumber seedling raising Fructus Cucumidis sativi and resisted low temperature ability
Select the cucumber seedling that 40 strain growing ways are identical, healthy, be divided into two groups and process 1.: by the concentration configured be
The alkali solution of beet root of 0.5kg/1000L executes cucumber seedling 24h, 2. compares and processes with clear water.Two groups of material low temperature at 6 DEG C
Coerce 2d, then measure malonaldehyde (MDA) content and erythrocuprein (SOD) activity, MDA content and the damage journey of cell membrane
Degree is proportionate, and MDA content is the fewest, then Plant chilling resistance is the best, and SOD activity is proportionate with plant life activity, SOD activity
The highest, Plant chilling resistance is the best.Result of the test see table 4:
Cucumber seedling MDA content and SOD activity situation under table 4 low temperature stress
Result of the test shows: in the case of 6 DEG C of low temperature stress 2d, process 1. in cucumber seedling MDA content substantially few
In comparison, reducing ratio is 39.7%, and SOD activity is significantly increased compared with the control, increase rate 59.0%.Garden beet alkali root is executed
Cucumber seedling can significantly improve Cucumber Chilling Resistance by Seedling Hardening.
Embodiment 4: under low temperature stress, glycine betaine soaks fruit raising banana cold resistance
Banana is divided into after single any of several broadleaf plants refers to, selects Maturity suppression, common without the banana of pest and disease damage and mechanical damage
40 are divided into two groups and process 1.: configuration concentration is the alkali solution of beet of 1kg/1000L, and one group of banana 20 is put into leaching
Take out after steeping 3 minutes and dry；Process 2. for comparison: another group banana 20 is put into and takes out after clear water soaks 3 minutes
Dry.Under 4 DEG C of low temperature, preserve banana 30 days, during storage researching determining banana damage to plants caused by sudden drop in temperature incidence rate and sarcocarp is hard
Degree, result see table 5, table 6:
Under table 5 low temperature stress, banana damages to plants caused by sudden drop in temperature incidence rate situation (%)
Banana pulp hardness situation (kg/cm under table 6 low temperature stress2)
Result of the test shows: banana damages to plants caused by sudden drop in temperature incidence rate and flesh firmness and is proportionate with by the low temperature stress time, 4
Under DEG C low temperature, comparison banana started from the 2nd day to occur damaging to plants caused by sudden drop in temperature, and the alkali-treated banana of Radix Betae is delayed and damages to plants caused by sudden drop in temperature appearance,
And glycine betaine processes banana and damages to plants caused by sudden drop in temperature the incidence rate banana significantly lower than concurrent control；Flesh firmness situation can reflect
The fruit resistivity to low temperature, as can be seen from Table 6, under identical cold preservation natural law, the banana sarcocarp that glycine betaine processes is hard
Degree is below compareing banana, and cold resistance significantly improves.Garden beet alkali soaks banana can be effectively improved banana
Resistivity to low temperature.
Above-described embodiment shows: use correct mode, processes the seed of plant with the alkali solution of beet of suitable concentration, individual
Body and fruit, it is possible to significantly improve its cold resistance, the scope of application is big, without potential safety hazard, can be widely applied to all kinds of plant raw
Produce and product is preserved.
Claims (1)
1. one kind utilizes glycine betaine to improve the method on Plant chilling resistance, it is characterised in that realized by following 4 kinds of modes:
1) seed soaking, before accelerating germination, configuration concentration is the aqueous solutions of betaine of 1～5kg/1000L, is put into by garbled seed and joins
In the alkali solution of beet put, liquid level seed to be exceeded 20～25 centimetres, soak seed 3～5 hours, select to add according to the thickness of seed coat
Appropriate hot water shortens seed soaking time, when, after seed imbibition, pulling seed immediately out and carry out accelerating germination；
2) root is executed, and at Seedling Stage or the period of duration of plant growing, executes with the alkali solution of beet root that concentration is 0.5～1kg/1000L
Plant, root executes time 0.5～2 hours, and the concrete time determines according to the seepage of water of soil or substrate, with complete permeable soil or base
Matter, enables plant root to fully absorb and is advisable；
3) foliage-spray, does medicament spray on plants blade face with the aqueous solutions of betaine that concentration is 0.05～40kg/1000L, specifically
Concentration determines according to the thin degree of plant leaf blade, and foliage-spray amount is advisable to there being droplet to drip to cover whole blade faces；
4) fruit soaks, and needs cryopreservation after picking fruit, and configuration concentration is that the glycine betaine of 0.5～2 kilogram/1000L is molten
Liquid, then puts into fruit to soak in solution 3～10 minutes and takes out, get final product cryopreservation after drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410763618.3A CN104396547B (en) | 2014-12-11 | 2014-12-11 | Method for improving chilling resistance of plant via glycine betaine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410763618.3A CN104396547B (en) | 2014-12-11 | 2014-12-11 | Method for improving chilling resistance of plant via glycine betaine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104396547A CN104396547A (en) | 2015-03-11 |
| CN104396547B true CN104396547B (en) | 2017-01-11 |
Family
ID=52634277
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410763618.3A Expired - Fee Related CN104396547B (en) | 2014-12-11 | 2014-12-11 | Method for improving chilling resistance of plant via glycine betaine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104396547B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104642047A (en) * | 2015-03-18 | 2015-05-27 | 民勤县天盛农业科技有限公司 | Culture method for high-quality and high-yield sandy Chinese red dates |
| CN108720073B (en) * | 2018-09-10 | 2021-04-06 | 云南省烟草农业科学研究院 | Flue-cured tobacco management and baking method for alleviating cold damage tobacco based on betaine application |
| CN108903044B (en) * | 2018-09-10 | 2020-12-22 | 云南省烟草农业科学研究院 | Flue-cured tobacco management and baking method for relieving gray tobacco based on betaine application |
| CN110226504B (en) * | 2019-07-12 | 2021-09-07 | 山西省农业科学院农作物品种资源研究所 | Cultivation method for improving stress resistance of wheat |
| CN113287506A (en) * | 2021-06-15 | 2021-08-24 | 河南农业大学 | Method for improving freezing resistance of tobacco seedlings |
| CN113940362A (en) * | 2021-09-29 | 2022-01-18 | 南京农业大学 | Wheat low-temperature-resistant protective agent product and use method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144637A (en) * | 2010-12-23 | 2011-08-10 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with cold-resistant function and application of composite |
| CN102487943A (en) * | 2011-12-14 | 2012-06-13 | 中国科学院遗传与发育生物学研究所 | Composition capable of increasing wheat yield, method of preparing composition and application of composition |
| CN104012534A (en) * | 2014-06-25 | 2014-09-03 | 中国科学院海洋研究所 | Wheat cold resistance regulating agent |
| CN104026132A (en) * | 2014-06-25 | 2014-09-10 | 中国科学院海洋研究所 | Method for preparing cold-resistance wheat conditioning agent |
| CN104094931A (en) * | 2014-07-31 | 2014-10-15 | 辽宁东亚种业有限公司 | Seed coating agent for improving cold resistance of maize seeds |
-
2014
- 2014-12-11 CN CN201410763618.3A patent/CN104396547B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144637A (en) * | 2010-12-23 | 2011-08-10 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with cold-resistant function and application of composite |
| CN102487943A (en) * | 2011-12-14 | 2012-06-13 | 中国科学院遗传与发育生物学研究所 | Composition capable of increasing wheat yield, method of preparing composition and application of composition |
| CN104012534A (en) * | 2014-06-25 | 2014-09-03 | 中国科学院海洋研究所 | Wheat cold resistance regulating agent |
| CN104026132A (en) * | 2014-06-25 | 2014-09-10 | 中国科学院海洋研究所 | Method for preparing cold-resistance wheat conditioning agent |
| CN104094931A (en) * | 2014-07-31 | 2014-10-15 | 辽宁东亚种业有限公司 | Seed coating agent for improving cold resistance of maize seeds |
Non-Patent Citations (2)
| Title |
|---|
| 外源甜菜碱对低温胁迫下香蕉内源甜菜碱合成的影响;李茂富等;《西北植物学报》;20110715;第31卷(第7期);第1400-1404页 * |
| 甜菜碱提高植物抗寒性的机理及其应用;韩冬芳等;《热带亚热带植物学报》;20100315;第20卷(第2期);第210=216页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104396547A (en) | 2015-03-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104396547B (en) | Method for improving chilling resistance of plant via glycine betaine | |
| CN104686265A (en) | Rice seedling raising method | |
| CN104025868B (en) | Pumpkin planting method | |
| CN103583190A (en) | Over-summer tomato greenhouse-cultivation method | |
| Hariprasanna | Foxtail millet: Nutritional importance and cultivation aspects | |
| CN103688706A (en) | Three-dimensional one-cultivation-two-harvest high-yield brewing glutinous sorghum cultivation method | |
| WO2015109880A1 (en) | Method for cultivating overyear regrowing rice seeds and seedling growing and planting method | |
| CN103444401A (en) | Method for planting ultra-large seed Chinese trichosanthess | |
| CN103283457A (en) | Drought-resisting batata seedling culture method | |
| CN101946772A (en) | Combined pesticide for controlling rice blast | |
| WO2022004741A1 (en) | Plant cultivation method and plant-vitalizing agent | |
| CN103283727B (en) | Method for preventing and treating rice red leaf | |
| CN104982295A (en) | Planting method for fast-growing lycium barbarum | |
| Abdulmalik et al. | Effects of moisture stress on flowering and fruit set in sweet pepper (Capsicum annuum L.) cultivars | |
| CN106800467A (en) | A kind of rainbow beans period foliar fertilizer spray agent | |
| El-Gazzar et al. | Effect of some treatments to reduce the injury of high temperature on sweet pepper grown in late summer season | |
| Bőcs et al. | Simultaneous impact of the different water supply and year type on processing tomato yield | |
| CN103975958A (en) | Special growth regulator for peanut seedling stage | |
| Ngwako et al. | The effect of irrigation and planting date on the growth and yield of Bambara groundnut landraces | |
| CN112299909A (en) | Fly-prevention plant protection compound agent for wheat in mature period | |
| CN102613188B (en) | Application of chitosan oligosaccharide in improvement of immunity of crops | |
| Srikanth et al. | Impact of Climate Change in Capsicum Production: A Review | |
| CN112493071B (en) | Seedling raising method for rapidly improving cold resistance of mechanical transplanted seedlings | |
| Morales et al. | Beet seedling emergence in function of sowing depth and system. | |
| Sharma | Cluster Bean (guar) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhu Xiaoli Inventor before: Li Runxia |
|
| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20161025 Address after: 730030 Gansu province Huachi County town kindergarten soft far Lane No. 6 Applicant after: Zhu Xiaoli Address before: 730726 Gansu province Baiyin County boss Li Ping Zhen Xiao Zhai village operation agency No. 87 Applicant before: Li Runxia |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170111 Termination date: 20211211 |