CN104396547A - Method for improving chilling resistance of plant via glycine betaine - Google Patents

Method for improving chilling resistance of plant via glycine betaine Download PDF

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Publication number
CN104396547A
CN104396547A CN201410763618.3A CN201410763618A CN104396547A CN 104396547 A CN104396547 A CN 104396547A CN 201410763618 A CN201410763618 A CN 201410763618A CN 104396547 A CN104396547 A CN 104396547A
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seed
plant
concentration
low temperature
root
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CN104396547B (en
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李润霞
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李润霞
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilizing, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154Organic compounds; Microorganisms; Enzymes

Abstract

The invention discloses a method for improving chilling resistance of plant via glycine betaine, and belongs to the technical field of pesticides. The method for improving the chilling resistance of the plant via the glycine betaine has the advantages that four methods of soaking the seeds with glycine betaine, applying soil to root, spraying to leaf surfaces or soaking the fruit are conducted to process the plant of different types, and thus the chilling resistance of the plant and fruit can be obviously improved; the applicable scope is wide, and no potential risk is caused.

Description

A kind of method utilizing betain to improve Plant chilling resistance
Technical field
The present invention relates to technical field of pesticide, specifically a kind of method utilizing betain to improve Plant chilling resistance.
Background technology
Temperature determines the main limiting factor of plant lower than distribution, is also the principal element affecting crops and horticultural crop yield and quality under cultivation condition.Damage to plants caused by sudden drop in temperature and refer to that above zero low temperature produces injury to plant corpus.Cause and damage to plants caused by sudden drop in temperature general 0 ~ 10 DEG C.Damage to plants caused by sudden drop in temperature the change causing a series of physiological metabolism in plant corpus, finally cause that growth of seedling is slim and frahile, plant strain growth is slow, wilting yellow, local necrosis, fruit-setting rate is low, and output reduces and the generation of degradation under quality.
Wheat is the important cereal crops of China, is mainly distributed in the north of north latitude 30 °.Can be divided into winter wheat and spring wheat according to habit of growth, the winter wheat relative spring wheat trophophase is long, from the trophophase to poor growth in reproduction period, experiences very long cold stage in winter therebetween.Winter Wheat Area, the north accounts for 59% of the national wheatland gross area, and the northern frequent cold air in this wheat district, winter, absolute temperature was low, and weather severe cold reduces the geographical distribution region of winter wheat suitable planting, made agricultural production suffer very large loss.
In addition, early spring, chilling injury was one of meteorological disaster main in corn agricultural production.Spring Maize Area, the north occurs often, and the underproduction more than 15% when disaster occurs, corn quality is also greatly affected.The vegetable crops such as cucumber, watermelon, tomato, growth is attacked by cold wave early stage, and its chlorenchyma is destroyed, and branch sprouts and is obstructed, growth retardation or stopping, and even plant is dead.
Through finding the literature search of prior art, the existing technology utilizing anti-cryogen to improve crop cold resistance, as Chinese patent Authorization Notice No. CN102511480B, patent name is: the cold-resistant type seed coat agent of temperature control sustained-release and cold-resistance agent preparation method.Anti-cryogen described in this patent, only for tobacco seed process, cannot take other processing modes to be applied to the plant except tobacco.Separately have the technology utilizing gene engineering to improve crop cold resistance, as Chinese patent Authorization Notice No. CN102115749B, patent name is: TT1 gene is improving the purposes in plant cold resistance.This patent adopts transgenic technology, uses recombinant vector transformed host plant cell, and screening obtains transformant, then transformant is cultivated into the cold-resistant plant of transgenosis.The transgenic product safety not yet sufficient proof that this technology is produced, should not be applied in crop production.
Summary of the invention
The object of the invention is to the drawback existed for existing method, propose a kind of method utilized on betain raising Plant chilling resistance, the present invention regulates the activity of Premeabilisation of cells pressure, protection biology enzyme by betain, thus improves plant to the resistivity of low temperature.
Realize technical scheme of the present invention as follows:
1) soak seed, before vernalization, configuration concentration is the aqueous solutions of betaine of 1 ~ 5kg/1000L, garbled seed is dropped in the alkali solution of beet of configuration, liquid level will exceed 20 ~ 25 centimetres, seed, soaks seed 3 ~ 5 hours, can select to add appropriate hot water shorten seed soaking time according to the thickness of seed coat, after Seed imbibes expands, pull seed immediately out and carry out vernalization.
2) root is executed, in Seedling Stage or the breeding time of plant growth, plant is executed with the alkali solution of beet root that concentration is 0.5 ~ 1kg/1000L, root executes 0.5 ~ 2 hour time, the concrete time is determined according to the seepage of water of soil or matrix, with complete permeable soil or matrix, plant root is fully absorbed as suitable.
3) foliage-spray, do medicament spray on plants blade face with the aqueous solutions of betaine that concentration is 0.05 ~ 40kg/1000L, concrete concentration is determined according to the thin degree of plant leaf blade, and foliage-spray amount is advisable to there being droplet to drip to cover whole blade face.
4) fruit soaks, and need low temperature storage after picking fruit, configuration concentration is the alkali solution of beet of 0.5 ~ 2 kilogram/1000L, then to be dropped into by fruit in solution to soak and takes out for 3 ~ 10 minutes, get final product low temperature storage after drying.
Described betain, improving the application on Plant chilling resistance, is characterized in that the present invention is directed to different plant, and the application method of betain and consumption difference, table 1 illustrates application method and the consumption of the common economic crops betain of part.
The mode of the different plant betaine administration of table 1 and concentration
Beneficial effect of the present invention is:
(1) the present invention is applicable to seed treatment before the vernalization of cereal, veterinary antibiotics, traditional Chinese medicine, water and fertilizer management after field planting, fruit storage after gathering, applied widely.
(2) the present invention soaks seed before being applied to plant vernalization, water and fertilizer management and fruit storage after gathering after field planting, all can significantly improve plant and fruit to the resistivity of low temperature, if low temperature stress is serious, can multi-pass operation, reach the object improving Plant chilling resistance, and plant can not develop immunity to drugs.
(3) described betain of the present invention belongs to non-poisonous material, for plant production and food processing process, without any potential safety hazard.
Specific implementation method
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1: under low temperature stress, aqueous solutions of betaine seed soaking improves Course of Corn Seed Germination ability
Process 1.: before vernalization, by boiling water and cold water half and half to good, get a certain amount of pure betain configuration aqueous solution, in 1000 liters, betain consumption 2 ~ 3 kilograms, dropped in aqueous solutions of betaine by 60 corn seeds chosen, liquid level will exceed 20 ~ 25 centimetres, seed, want to stir with waddy in time simultaneously, seed is heated evenly.Water temperature will control at 25 ~ 28 degrees Celsius, soaks seed 3 ~ 5 hours under this temperature condition, after Seed imbibes expands, pulls seed out and is equally divided into 2 groups; 2. process is contrast: before vernalization, by boiling water and cold water half and half to good, and dropped in warm water by 60 corn seeds chosen, the water surface will exceed 20 ~ 25 centimetres, seed, wants to stir with waddy in time simultaneously, and seed is heated evenly.Water temperature will control at 25 ~ 28 degrees Celsius, soaks seed 3 ~ 5 hours under this temperature condition, after Seed imbibes expands, pulls seed out and is equally divided into 2 groups.By changing low temperature stress temperature and low temperature stress time, respectively to 2 process totally 4 groups of seeds carry out germination test, testing program and result are see with following table 2:
Table 2 difference is endured cold the germination rate situation (%) of seed under time, Different hypothermia process
Result of the test shows: the percentage of seedgermination of two kinds of process becomes negative correlation with number of days of enduring cold, under the condition that number of days of enduring cold is identical ?under 10 DEG C of low temperature two kinds of process seeds germination rate no significant difference , ?process 1. seed under 20 DEG C of low temperature germination rate 2. exceed about 6 times than process.The seed soaking of the garden beet aqueous slkali aqueous solution is significantly increased to the cold resistance of corn seed.
Embodiment 2: under low temperature stress, foliage-spray betain improves wheat vitality
Configuration concentration is the alkali solution of beet of 30kg/1000L, select 40 strain growing ways identical, wheat plant without damage by disease and insect is divided into two groups, carries out respectively processing 1.: foliage-spray betain, and 2. process contrasts: foliage-spray clear water, foliage-spray amount is dripped to there being droplet to cover whole blade face, natural air drying absorbs, and after all wheat leaf blade surfaces are all without moisture, puts low temperature stress 12h in ?2 DEG C of insulating boxs, measure photosynthesis with the photosynthetic instrument of EGM-4, calculate blade lethality.Result of the test sees the following form 3:
The photosynthesis of wheat and blade lethality situation under table 3 low temperature stress
Result of the test show: ?after 2 DEG C of low temperature stress 12h, 1. compared with the control, light and total amount improve 57.1%, and blade lethality reduces by 38.5% in process.Wheat foliage-spray betain effectively can alleviate the harm of low temperature stress, improves wheat vitality.
Embodiment 3: under low temperature stress, betain root is executed cucumber seedling raising cucumber and resisted low temperature ability
Select the cucumber seedling that 40 strain growing ways are identical, healthy, be divided into two groups and process 1.: execute cucumber seedling 24h with the alkali solution of beet root that the concentration configured is 0.5kg/1000L, 2. contrast and use clear water process.Two groups of material low temperature stress 2d at 6 DEG C, then malonaldehyde (MDA) content and erythrocuprein (SOD) activity is measured, the damaged condition of MDA content and cell membrane is proportionate, MDA content is fewer, then Plant chilling resistance is better, SOD is active to be proportionate with plant life activity, and SOD activity is higher, and Plant chilling resistance is better.Result of the test sees the following form 4:
Cucumber seedling MDA content and the active situation of SOD under table 4 low temperature stress
Result of the test shows: when being subject to 6 DEG C of low temperature stress 2d, process 1. in cucumber seedling MDA content be obviously less than contrast, reducing ratio is that 39.7%, SOD activity is significantly increased compared with the control, increase rate 59.0%.Garden beet alkali root executes cucumber seedling can significantly improve Cucumber Chilling Resistance by Seedling Hardening.
Embodiment 4: under low temperature stress, betain soaks fruit raising banana cold resistance
Banana is divided into after single any of several broadleaf plants refers to, select maturity to suppress, without damage by disease and insect and mechanical damage banana totally 40 be divided into two groups and process 1.: configuration concentration is the alkali solution of beet of 1kg/1000L, one group of banana 20 is put into immersion and takes out after 3 minutes and dry; 2. process is contrast: another group banana 20 is put into clear water and soak to take out after 3 minutes and dry.Under 4 DEG C of low temperature, preserve banana 30 days, duration of storage researching determining banana damage to plants caused by sudden drop in temperature incidence and flesh firmness, the results are shown in following table 5, table 6:
Under table 5 low temperature stress, banana damages to plants caused by sudden drop in temperature incidence situation (%)
Banana pulp hardness situation (kg/cm under table 6 low temperature stress 2)
Result of the test shows: banana damages to plants caused by sudden drop in temperature incidence and flesh firmness and is proportionate by the low temperature stress time, under 4 DEG C of low temperature, contrast banana occurred damaging to plants caused by sudden drop in temperature from the 2nd day, the alkali-treated banana of beet is delayed and damages to plants caused by sudden drop in temperature appearance, and betain process banana damages to plants caused by sudden drop in temperature the banana that incidence is starkly lower than concurrent control; Flesh firmness situation can reflect the resistivity of fruit to low temperature, and as can be seen from Table 6, under identical refrigeration number of days, the banana flesh firmness of betain process is all lower than contrast banana, and cold resistance significantly improves.Garden beet alkali soaks banana effectively can improve the resistivity of banana to low temperature.
Above-described embodiment shows: adopt correct mode, with the seed of the alkali solution of beet process plant of suitable concentration, and individual and fruit, its cold resistance can be significantly improved, the scope of application is large, without potential safety hazard, can be widely used in all kinds of plant production and product storage.

Claims (1)

1. utilize the method on betain raising Plant chilling resistance, it is characterized in that, realized by following 4 kinds of modes:
1) soak seed, before vernalization, configuration concentration is the aqueous solutions of betaine of 1 ~ 5kg/1000L, garbled seed is dropped in the alkali solution of beet of configuration, liquid level will exceed 20 ~ 25 centimetres, seed, soaks seed 3 ~ 5 hours, can select to add appropriate hot water shorten seed soaking time according to the thickness of seed coat, after Seed imbibes expands, pull seed immediately out and carry out vernalization.
2) root is executed, in Seedling Stage or the breeding time of plant growth, plant is executed with the alkali solution of beet root that concentration is 0.5 ~ 1kg/1000L, root executes 0.5 ~ 2 hour time, the concrete time is determined according to the seepage of water of soil or matrix, with complete permeable soil or matrix, plant root is fully absorbed as suitable.
3) foliage-spray, do medicament spray on plants blade face with the aqueous solutions of betaine that concentration is 0.05 ~ 40kg/1000L, concrete concentration is determined according to the thin degree of plant leaf blade, and foliage-spray amount is advisable to there being droplet to drip to cover whole blade face.
4) fruit soaks, and need low temperature storage after picking fruit, configuration concentration is the alkali solution of beet of 0.5 ~ 2 kilogram/1000L, then to be dropped into by fruit in solution to soak and takes out for 3 ~ 10 minutes, get final product low temperature storage after drying.
CN201410763618.3A 2014-12-11 2014-12-11 Method for improving chilling resistance of plant via glycine betaine Active CN104396547B (en)

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CN104642047A (en) * 2015-03-18 2015-05-27 民勤县天盛农业科技有限公司 Culture method for high-quality and high-yield sandy Chinese red dates
CN108720073A (en) * 2018-09-10 2018-11-02 云南省烟草农业科学研究院 A kind of flue-cured tobacco management and baking method damaging to plants caused by sudden drop in temperature cigarette using alleviation based on glycine betaine
CN108903044A (en) * 2018-09-10 2018-11-30 云南省烟草农业科学研究院 It is a kind of to apply flue-cured tobacco management and the baking method for alleviating grey cigarette based on glycine betaine
CN110226504A (en) * 2019-07-12 2019-09-13 山西省农业科学院农作物品种资源研究所 A kind of cultural method promoting Resistance of Wheat To Adversity

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104642047A (en) * 2015-03-18 2015-05-27 民勤县天盛农业科技有限公司 Culture method for high-quality and high-yield sandy Chinese red dates
CN108720073A (en) * 2018-09-10 2018-11-02 云南省烟草农业科学研究院 A kind of flue-cured tobacco management and baking method damaging to plants caused by sudden drop in temperature cigarette using alleviation based on glycine betaine
CN108903044A (en) * 2018-09-10 2018-11-30 云南省烟草农业科学研究院 It is a kind of to apply flue-cured tobacco management and the baking method for alleviating grey cigarette based on glycine betaine
CN108720073B (en) * 2018-09-10 2021-04-06 云南省烟草农业科学研究院 Flue-cured tobacco management and baking method for alleviating cold damage tobacco based on betaine application
CN110226504A (en) * 2019-07-12 2019-09-13 山西省农业科学院农作物品种资源研究所 A kind of cultural method promoting Resistance of Wheat To Adversity

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