CN103493725A - Kiwi fruit artificial pollination method and preparing method of kiwi fruit pollen - Google Patents
Kiwi fruit artificial pollination method and preparing method of kiwi fruit pollen Download PDFInfo
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Abstract
The invention discloses a preparing method of kiwi fruit pollen. The method includes the following steps: (1) male plants and female plants are configured according to the quantity ratio of 19-21:1, the male plants are cultured in a facility greenhouse, and the female plants are cultured in a field through shed frames; (2) after the male plants enter a flowering period, male flowers which are to blossom are selected, anthers in the male flowers are taken out, spread, dried and crushed, and then the kiwi fruit pollen is obtained; (3) the storage period of the kiwi fruit pollen is 1 week at a normal temperature, and the storage period of the kiwi fruit pollen is 30-40 days at a refrigerated storage temperature. The invention further discloses a kiwi fruit artificial pollination method, wherein artificial pollination is carried out by means of the kiwi fruit pollen in the storage period after female flowers enter a flowering period. The kiwi fruit artificial pollination method includes that in the early stage of blossoming, a point pollination method is adopted through a pollination pen, and a dry powder spraying method is adopted through a spraying-type pollination device in a full-blossom period. According to the kiwi fruit artificial pollination method and the preparing method of the kiwi fruit pollen, the high fruit setting rate and the high fruit quality in kiwi fruit production are guaranteed.
Description
Technical field
The invention belongs to agriculture field, particularly a kind of Kiwi fruit pollen production technology and artificial pollination maneuver.
Background technology
Kiwi berry nutritious abundant, be rich in vitamin C and various trace element, its pulp is delicate, give off a strong fragrance, mouthfeel fragrant and sweet salubrious, acidity is extremely low, and " title of magical delicious fruit " arranged.In recent years, the development speed of China's kiwi fruit was very fast, and current national cultivated area has reached more than 500,000 mu.Current existing kiwi fruit is male and female plant mixed culture pattern,, is conventional land for growing field crops pergola system that is.
Because kiwi fruit is dioecian plant, want to obtain the fruit of higher output and high-quality on producing, artificial pollination is requisite measure, especially the time at florescence of kiwi fruit short, rely on open-pollinated to be difficult to guarantee good quality and high output.But, in actual production, the time that the Actinidia strain is bloomed often is subject to the impact of Different climate condition, and does not meet, while causing artificial pollination without pollen or pollen is arranged and female plant is not bloomed.In addition, the duration of blooming due to kiwi fruit is about 7 days, and the pollination time of female flower the best only has the time limit of 2～3 days, and the artificial pollination workload is large, if run into the Pluvial more difficult pollination of blooming, orchard this defect slightly large at area seems more outstanding.Therefore, necessaryly existing Kiwi fruit pollen production technology and artificial pollination method of operating are innovated to improvement, the fruit-setting rate that the production of guarantee kiwi fruit is higher and fruit quality.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of kiwi fruit artificial pollination method and corresponding Kiwi fruit pollen preparation method, adopts method of the present invention can guarantee that kiwi fruit production has higher fruit-setting rate and fruit quality.
In order to solve the problems of the technologies described above, the invention provides a kind of Kiwi fruit pollen preparation method, comprise the following steps:
A, male and female plant are preferably 20:1 according to 19～21:1() the quantity ratio be configured, staminiferous plant is facility plastic greenhouse cultivation, female plant is the land for growing field crops pergola system;
B, after staminiferous plant enters flowering stage, carry out the collection of pollen, carry out successively following steps:
Pluck male flower: before 9, the male flower that selection will be opened (that is the male flower that, presents " small bell " shape), pluck from anthocaulus in the morning;
Get flower pesticide and the flower pesticide that dries in the air: carry out airing after taking out the flower pesticide tiling in male flower, until flower pesticide can not cohere mutually, finish airing;
Sieve pollen and storage: the flower pesticide after airing is crushed to and can crosses at least 150 purpose sieves; Then put into container stores or stores under the refrigerated storage temperature of 0～5 ℃ under the normal temperature of 10～25 ℃; Obtain Kiwi fruit pollen;
Be 1 week the storage period under described normal temperature, and be 30～40 days the storage period under described refrigerated storage temperature.
Improvement as Kiwi fruit pollen preparation method of the present invention:
Steps A is for carrying out successively following steps:
A), female plant and staminiferous plant are configured according to the quantity ratio of 19～21:1;
B), staminiferous plant is facility plastic greenhouse cultivation, seeding row spacing is 3 meters * 2 meters; Female plant is the land for growing field crops pergola system, 4 meters * 3 meters of seeding row spacings;
Described facility plastic greenhouse cultivation, for the planting type of plastic film shed, when kiwi fruit enters leaf stretching period, is detained canopy to staminiferous plant and is processed.
The remarks explanation: all the other planting patternss are with the plantation of conventional kiwi fruit.
The kiwi fruit artificial pollination method that the present invention also provides a kind of Kiwi fruit pollen that utilizes the said method preparation and obtain to carry out simultaneously, after female flower enters flowering stage, utilization is positioned at the Kiwi fruit pollen of storage period and carries out artificial pollination; Comprise:
When coming into bloom (the Ji Quan female plant amount of blooming in garden<30% time), when female plant does not have large tracts of land to bloom, adopt pollination pen point to award method (for the method I): when female flower is open, morning 7 on non-rainy day～10 or pollinated afternoon 4～7;
When full-bloom stage (the Ji Quan female plant amount of blooming in garden >=30% time), when the female plant large tracts of land is bloomed, adopt atomizing pollenator dry powder spraying method (be the method II): when the female flower opening, morning 7 on non-rainy day～10 or pollinated afternoon 4～7.
The remarks explanation: above-mentioned 2 kinds of methods are used in conjunction with.
Improvement as kiwi fruit artificial pollination method of the present invention:
The described pollination pen point method of awarding is: after making the light libation at an ancient wedding ceremony pollen of pollination pen, touch pistil stigma, thereby realize pollination.
Described atomizing pollenator dry powder spraying method is:
After Kiwi fruit pollen and water milling powder or glutinous rice flour are evenly mixed according to the mass ratio of 1:1, obtain the pollen mixture; The pollen mixture is placed in the atomizing pollenator, makes the spout of atomizing pollenator be positioned at 5～8 centimetres of female flower below; Thereby realize Kiwi fruit pollen is sprayed onto to female taking.
Technical problem solved by the invention and corresponding technical advantage are:
One), changed the cultivation mode of traditional kiwi fruit production according to the configuration of male and female plant certain proportion, but produce the required pollen quantity of area according to kiwi fruit, carry out the facility plastic greenhouse cultivation of staminiferous plant in the defined area, production base, concentrate the production of carrying out pollen, other zone in base is field planting female plant (being conventional land for growing field crops pergola system) all.Adopt this cultivation mode can concentrate the production of carrying out Kiwi fruit pollen, and cultivate and can make the staminiferous plant Blooming by facility plastic greenhouse, pollen after collection can be preserved (refrigeration of 0～5 ℃ is best mode) by the refrigeration of 0～5 ℃ or the mode of normal temperature, when entering flowering stage, female plant carries out artificial pollination, while having avoided the kiwi fruit female plant to bloom, staminiferous plant does not bloom, and there is no the predicament of pollen and its pollination.
The remarks explanation: under existing male and female plant mixed culture pattern, the male and female plant allocation ratio is 8～10:1.
Two), artificial pollination method can adopt pollination pen point to award the mode that method and/or atomizing pollenator dry powder spraying method combine rationally to carry out artificial pollination.
It is good that pollination pen point is awarded the method Pollination Effect, but more time-consuming taking a lot of work; And pollenator dry powder spraying method time and labour saving, but need pollen amount more.In actual production, female plant comes into bloom and blooms number seldom, and now suitable employing pollination pen point is awarded method; If, but weather is fine continuously, female plant enters full-bloom stage very soon, a large amount of female flowers need artificial pollination, now suitable employing pollenator dry powder spraying method.
Adopt aforesaid way, can guarantee that kiwi fruit production has higher fruit-setting rate and fruit quality.
The accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is a kind of atomizing pollenator that can obtain by commercial mode that the present invention selects.
Embodiment
Below in conjunction with example, the present invention will be further described.
One, Kiwi fruit pollen preparation method, carry out following steps (plantation in Zhejiang Province of take is example) successively:
The plantation of A, male and female plant:
A), at the beginning of annual December to next year 3, by 4 meters * 3 meters of seeding row spacings, carry out the field planting female plant, that is, female plant is the land for growing field crops pergola system; Carry out the field planting staminiferous plant in the zone that can build plastic tunnel according to 3 meters * 2 meters of seeding row spacings, that is, staminiferous plant is the facility plastic greenhouse cultivation;
The quantity of the staminiferous plant of field planting configures in the ratio of male and female plant 20:1.
B), be annual mid or late April (under the state of land for growing field crops) flowering stage due to area, Zhejiang Province red kiwi fruit, therefore staminiferous plant starts to detain canopy (be generally in Zhejiang Province annual March 15 before and after be leaf stretching period) when kiwi fruit enters leaf stretching period, now needs staminiferous plant is detained to canopy (being covered with plastic film);
The remarks explanation: staminiferous plant additionally need to be detained outside canopy, and the measure of other cultivation is the same with female plant, is conventional kiwi fruit plant culture mode.
After B, staminiferous plant button canopy, generally, through the time of about 30 days, start to enter flowering stage; In Zhejiang Province, be generally before and after annual April 15.Now, continue to carry out the collection of pollen according to following steps, the time that can gather pollen is about 7 days:
Pluck male flower: 9 were carried out in the past in the morning, and the male flower that selection will be opened (male flower that presents " small bell " shape) is plucked and brought back from anthocaulus.
Get flower pesticide: first get two clean newspapers, divide on underground or table, by the male flower of adopting back, break petal into two with one's hands, with hand, pluck or with toothbrush, its flower pesticide is brushed on newspaper.
Flower pesticide dries in the air: the flower pesticide under brushing is put into drying, ventilation airing.As sombre weather, can be put on electric blanket or under the lamp of heating and heat, dry (note, heating-up temperature can not over 40 ℃); Until pollen does not cohere mutually, finish airing.
Sieve pollen and storage: just the flower pesticide after airing is crushed to and can crosses at least 150 purpose sieves; Then the storage of packing in clean bottle is standby.Normal temperature (10～25 ℃) is lower can be stored 1 week, or can keep Pollen Activity 30～40 days by 0～5 ℃ of refrigeration.
To be storage period 0 day, store 1 week under 25 ℃, the Kiwi fruit pollen of storing 30～40 days under 0～5 ℃ of refrigeration carries out the detection of following vigor performance:
Pollen Activity is measured and is adopted the tetrazolium salts method: the index that the pollen staining rate of usining is studied as Pollen Activity.Chlorinated triphenyl base tetrachloro azoles (TTC) with 4% adds pollen grain and makes section, is placed in the baking oven of 37 ℃, is positioned over the optical microphotograph Microscopic observation after 30min, shows the red great-hearted pollen that is.Repeat for 3 times, 3 visuals field of each repeated observation, calculate its dyeing percentage.
Result is respectively: the pollen that be 0 day storage period (just having plucked) pollen staining rate is 83.6%; Pollen staining rate in the pollen of storing under 25 ℃ 1～7 day is more than 78.1%, and the pollen staining rate more than 8 days is below 20%; 0～5 ℃ of refrigeration pollen 40 days with interior pollen staining rate more than 72.1%, and after 40 days the pollen staining rate below 25%.
Two, artificial pollination
In Zhejiang Province, the red kiwi fruit of land for growing field crops pergola system enters flowering stage after generally before annual April 20, the Kiwi fruit pollen that now utilizes above-mentioned steps one to preserve carries out artificial pollination according to following two kinds of methods, and wherein at female flower, pollination in open the 2nd to the 3rd day is the best.
Remarks explanations: petal when female flower is open be that a bud just ready to burst state, very obviously, can know that second day opens certainly, a flower can be opened 2～6 days, full-bloom stage mostly is 3～4 days.When just open, the bract of flower does not open fully, and the complete state that the flowers are in blossom is to open fully, and pattern is bright-coloured, and after the flowers are in blossom couple of days, what pattern will become is not very bright-coloured, and tension force is not tall and straight in the past yet.After pollination, the petal of white can become faint yellow very soon.
When coming into bloom (the Ji Quan female plant amount of blooming in garden<30% time), when female plant does not have large tracts of land to bloom, adopt pollination pen point to award method (method I):
When full-bloom stage (the Ji Quan female plant amount of blooming in garden >=30% time), when the female plant large tracts of land is bloomed, adopt atomizing pollenator dry powder spraying method (method II).
Specific as follows:
The artificial pollination method I: pollination pen point is awarded method, and suitable coming into bloom, do not adopt when female plant has large tracts of land to bloom.Comprise following content:
Make the pollination pen: adopt common writing brush, cut off the apicule part, the pen hair is neatly got final product, or adopt the sponge in cigarette filter, the fine bamboo rod that bind is 20 centimetres, waddy.
Field pollination: ready pollen (Kiwi fruit pollen of above-mentioned steps one gained) is poured out on a small quantity, in the clean teacup of packing into.When female flower is open, wait revealing after (rain) water is done can pollinate (morning 7～10 points, afternoon 4～7 pollination are advisable, and can not pollinate rainy day).
Concrete pollination operating technology: take on the other hand the pollen cup during pollination, take on the other hand the pollination pen, after light libation at an ancient wedding ceremony pollen, to pistil stigma gently docking point award and get final product.The general libation at an ancient wedding ceremony is got a pollen and can be put and award 5～7 female flowers.
Remarks explanation: adopt above-mentioned artificial pollination method I, 1 mu of pure pollen of need 7～8 gram.
The artificial pollination method II: Kiwi pollinator dry powder spraying method, Kiwi pollinator is selected atomizing pollenator succinct on market, as shown in Figure 1.This atomizing pollenator holds bottle 1 by pollen and air-blowing capsule 2 forms.This method is suitable to full-bloom stage, when the female plant large tracts of land is bloomed, adopts.Comprise following content:
The making of pollen dry powder: the Kiwi fruit pollen that above-mentioned steps one is preserved mixes according to the 1:1 ratio with water milling powder or glutinous rice flour, and the pollen of pouring Kiwi pollinator into holds in bottle 1 and gets final product.
The field operating technology of specifically pollinating: when female flower is open, wait revealing (rain) water can pollinate after dry (morning 7～10 points, afternoon 4～7 pollination are advisable, and can not pollinate rainy day).During pollination, the Kiwi pollinator of the hand-held pollen of packing into, below female flower, 5～8 centimeters are sprayed onto female taking equably by pollen.Spray once can pollinate to 15～20 female flowers simultaneously simultaneously.
The remarks explanation: by above-mentioned artificial pollination method II, 1 mu of pure pollen of need 14～16 gram.
All the other cultivation conditions of red kiwi fruit are with the cultivation condition of existing conventional red kiwi fruit.
Adopt aforesaid way, kiwi fruit production has higher fruit-setting rate and fruit quality.Be specially: within after pollination one week, measure fruit-setting rate and can reach more than 90%, the rear mensuration fruit quality of gathering as: the fruit single fruit weight on average can reach 93 grams and (not use 93 grams in any somatotropin situation, use under the somatotropin condition and can reach 112 grams), soluble solid content can reach 20.8%.
One, Kiwi fruit pollen preparation method, carry out following steps (plantation in Zhejiang Province of take is example) successively:
The plantation of A, male and female plant:
A), selected Kiwifruit Cultivars is the Xu Xiang kiwi fruit, at the beginning of annual December to next year 3, by 4 meters * 3 meters of seeding row spacings, carries out the field planting female plant, that is, female plant is the land for growing field crops pergola system; The field planting staminiferous plant is carried out according to 3 meters * 2 meters of seeding row spacings in zone at the frame of can puing up a shed, that is, staminiferous plant is the facility plastic greenhouse cultivation; The quantity of field planting staminiferous plant configures in the ratio of male and female plant 20:1.
B), at the beginning of by the end of April to 5 that be every year month in the flowering stage of Zhejiang Province area Xu Xiang kiwi fruit (under the state of land for growing field crops), staminiferous plant generally starts to detain canopy when kiwi fruit enters the exhibition leaf, in Zhejiang Province, be generally before and after annual March 25, other cultivation step that need to detain canopy except staminiferous plant is the same with female plant;
After B, staminiferous plant button canopy, generally, through the time of about 30 days, start to enter flowering stage, be generally in Zhejiang Province before and after annual April 25..Now, continue to carry out the collection of pollen according to following steps, the time that can gather pollen is about 7 days:
Pluck male flower: 9 were carried out in the past in the morning, and the male flower that selection will be opened (male flower that presents " small bell " shape) is plucked and brought back from anthocaulus.
Get flower pesticide: first get two clean newspapers, divide on underground or table, by the male flower of adopting back, break petal into two with one's hands, with hand, pluck or with toothbrush, its flower pesticide is brushed on newspaper.
Flower pesticide dries in the air: the flower pesticide under brushing is put into drying, ventilation airing.As sombre weather, can be put on electric blanket or under the lamp of heating and heat, dry (note, heating-up temperature can not over 40 ℃); Until pollen does not cohere mutually, finish airing.
Sieve pollen and storage: just the flower pesticide after airing is crushed to and can crosses at least 150 purpose sieves; Then the storage of packing in clean bottle is standby.Normal temperature (10～25 ℃) is lower can be stored 1 week, or can keep Pollen Activity 30～40 days by 0～5 ℃ of refrigeration.
Two, artificial pollination
In Zhejiang Province, the Xu Xiang kiwi fruit enters flowering stage after generally before annual May 1, now utilizes the pollen of gained in above-mentioned steps to carry out artificial pollination according to following two kinds of methods, and wherein at female flower, pollination in open the 2nd to the 3rd day is hung most.
The artificial pollination method I: pollination pen point is awarded method, suitable coming into bloom (the Ji Quan female plant amount of blooming in garden<30% time), that is, when female plant has large tracts of land to bloom, adopt.
Make the pollination pen: adopt common writing brush, cut off the apicule part, the pen hair is neatly got final product; Perhaps adopt the sponge in cigarette filter, the fine bamboo rod that bind is 20 centimetres, waddy.
Field pollination: ready pollen is poured out on a small quantity, in the clean teacup of packing into.When female flower is open, wait revealing after (rain) water is done can pollinate (morning 7～10 points, afternoon 4～7 pollination are advisable, and can not pollinate rainy day).
Concrete pollination operating technology: take on the other hand the pollen cup during pollination, take on the other hand the pollination pen, after light libation at an ancient wedding ceremony pollen, to pistil stigma gently docking point award and get final product.The general libation at an ancient wedding ceremony is got a pollen and can be put and award 5～7 female flowers.
Remarks explanation: adopt above-mentioned artificial pollination method I, 1 mu of pure pollen of need 7～8 gram.
The artificial pollination method II: Kiwi pollinator dry powder spraying method, Kiwi pollinator is selected atomizing pollenator succinct on market, as shown in Figure 1.This method is suitable to full-bloom stage (the Ji Quan female plant amount of blooming in garden >=30% time), that is, when the female plant large tracts of land is bloomed, adopt.
The making of pollen dry powder: the pollen of above-mentioned steps gained is mixed according to the 1:1 ratio with water milling powder or glutinous rice flour, pour Kiwi pollinator into and get final product.
The field operating technology of specifically pollinating: when female flower is open, wait revealing (rain) water can pollinate after dry (morning 7～10 points, afternoon 4～7 pollination are advisable, and can not pollinate rainy day).During pollination, the Kiwi pollinator of the hand-held pollen of packing into, below female flower, 5～8 centimeters are sprayed onto female taking equably by pollen.Spray once can pollinate to 15～20 female flowers simultaneously simultaneously.
The remarks explanation: by above-mentioned artificial pollination method II, 1 mu of pure pollen of need 14～16 gram.
All the other cultivation conditions of Xu Xiang kiwi fruit are with the cultivation condition of existing conventional Xu Xiang kiwi fruit.
Adopt aforesaid way, kiwi fruit production has higher fruit-setting rate and fruit quality.Being specially the pollination after one week mensuration fruit-setting rate can reach more than 90%, the rear mensuration fruit quality of gathering as: the fruit single fruit weight on average can reach 108 grams, and soluble solid content can reach 17%.
Comparative Examples 1, the red kiwi fruit described in embodiment 1 is planted according to prior art, that is, at the beginning of annual December to next year 3, by 4 meters * 3 meters of seeding row spacings, carried out field planting female plant and staminiferous plant; The ratio of male and female plant is 10:1; Planted in a conventional manner, the artificial pollination mode is to put honeybee in spontaneous pollination or orchard.
As a result, the fruit-setting rate of kiwi fruit is only 76%, and it is generally less that fruit quality shows as the fruit single fruit weight, average 73.6 grams, and size is all uneven, and soluble solid content is generally in 19% left and right.
Comparative Examples 2, the Xu Xiang kiwi fruit described in embodiment 2 is planted according to prior art, that is, at the beginning of annual December to next year 3, by 4 meters * 3 meters of seeding row spacings, carried out field planting female plant and staminiferous plant; The ratio of male and female plant is 10:1; Planted in a conventional manner, the artificial pollination mode is to put honeybee in spontaneous pollination or orchard.
As a result, the fruit-setting rate of kiwi fruit is only 78%, and it is generally less that fruit quality shows as the fruit single fruit weight, average 80.6 grams, and size is all uneven, and soluble solid content is generally in 15.8% left and right.
Finally, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (5)
1. Kiwi fruit pollen preparation method is characterized in that comprising the following steps:
A, male and female plant are configured according to the quantity ratio of 19～21:1, and staminiferous plant is the facility plastic greenhouse cultivation, and female plant is the land for growing field crops pergola system;
B, after staminiferous plant enters flowering stage, carry out the collection of pollen, carry out successively following steps:
Pluck male flower: before 9, the male flower that selection will be opened, pluck from anthocaulus in the morning;
Get flower pesticide and the flower pesticide that dries in the air: carry out airing after taking out the flower pesticide tiling in male flower, until flower pesticide can not cohere mutually, finish airing;
Sieve pollen and storage: the flower pesticide after airing is crushed to and can crosses at least 150 purpose sieves; Then put into container stores or stores under the refrigerated storage temperature of 0～5 ℃ under the normal temperature of 10～25 ℃; Obtain Kiwi fruit pollen;
Be 1 week the storage period under described normal temperature, and be 30～40 days the storage period under described refrigerated storage temperature.
2. Kiwi fruit pollen preparation method according to claim 1 is characterized in that:
Described steps A is for carrying out successively following steps:
A), female plant and staminiferous plant are configured according to the quantity ratio of 19～21:1;
B), staminiferous plant is facility plastic greenhouse cultivation, seeding row spacing is 3 meters * 2 meters; Female plant is the land for growing field crops pergola system, 4 meters * 3 meters of seeding row spacings;
Described facility plastic greenhouse cultivation, for the planting type of plastic film shed, when kiwi fruit enters leaf stretching period, is detained canopy to staminiferous plant and is processed.
3. the kiwi fruit artificial pollination method that the Kiwi fruit pollen that utilizes method preparation as claimed in claim 1 or 2 and obtain carries out, it is characterized in that: after female flower enters flowering stage, utilization is positioned at the Kiwi fruit pollen of storage period and carries out artificial pollination; Comprise:
When coming into bloom, when female plant does not have large tracts of land to bloom, adopt pollination pen point to award method: when female flower is open, morning 7 on non-rainy day～10 or pollinated afternoon 4～7;
Work as full-bloom stage, when the female plant large tracts of land is bloomed, adopt atomizing pollenator dry powder spraying method: when female flower is open, morning 7 on non-rainy day～10 or pollinated afternoon 4～7.
4. kiwi fruit artificial pollination method according to claim 3 is characterized in that:
The described pollination pen point method of awarding is:
After making the light libation at an ancient wedding ceremony pollen of pollination pen, touch pistil stigma, thereby realize pollination.
5. kiwi fruit artificial pollination method according to claim 3 is characterized in that:
Described atomizing pollenator dry powder spraying method is:
After Kiwi fruit pollen and water milling powder or glutinous rice flour are evenly mixed according to the mass ratio of 1:1, obtain the pollen mixture; The pollen mixture is placed in the atomizing pollenator, makes the spout of atomizing pollenator be positioned at 5～8 centimetres of female flower below; Thereby realize Kiwi fruit pollen is sprayed onto to female taking.
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| CN114009270A (en) * | 2021-12-01 | 2022-02-08 | 全椒县绿兴园生态农业发展有限公司 | Artificial pollination technology for apocarya |
| CN114451292A (en) * | 2022-02-17 | 2022-05-10 | 浙江省农业科学院 | Pollen preparation technology for seed softening and reduction of tetraploid Chinese gooseberry |
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| CN104770289A (en) * | 2015-05-11 | 2015-07-15 | 四川中新农业科技有限公司 | Method for making pollen and pollinating flowers under large-scale planting condition |
| CN104938272A (en) * | 2015-06-02 | 2015-09-30 | 张亚南 | Planting method for organic kiwi fruits |
| CN105567783A (en) * | 2016-03-10 | 2016-05-11 | 中国农业科学院特产研究所 | Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta |
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| CN109618914A (en) * | 2019-01-16 | 2019-04-16 | 广西壮族自治区中国科学院广西植物研究所 | A kind of preparation method of Siraitia grosvenorii spraying type pollinating method and pollen |
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| CN109769567B (en) * | 2019-03-07 | 2021-03-30 | 三明市农业科学研究院 | Cultivation method for improving seed setting rate of skimmia purpurea |
| CN113728915A (en) * | 2021-09-06 | 2021-12-03 | 贵州省山地资源研究所 | Preparation method of kiwi pollen |
| CN114009270A (en) * | 2021-12-01 | 2022-02-08 | 全椒县绿兴园生态农业发展有限公司 | Artificial pollination technology for apocarya |
| CN114451292A (en) * | 2022-02-17 | 2022-05-10 | 浙江省农业科学院 | Pollen preparation technology for seed softening and reduction of tetraploid Chinese gooseberry |
| CN114766353A (en) * | 2022-05-09 | 2022-07-22 | 辽宁省经济林研究所 | Rapid screening method and application of male pollination plants in actinidia arguta orchard |
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