CN106212267B - A kind of Alnus cremastogyne male inflorescence rapid draing and pollen efficient collecting method - Google Patents
A kind of Alnus cremastogyne male inflorescence rapid draing and pollen efficient collecting method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a kind of Alnus cremastogyne male inflorescence rapid draing and pollen efficient collecting methods, include the following steps：(1) inflorescence is collected：Acquire the male inflorescence of 4~5 centimeter length of Alnus cremastogyne；(2) assembling of inflorescence drying frame；(3) inflorescence is put：The male inflorescence of collected 4~5 centimeter length is laid in A types to dry on the parchment of frame；(4) inflorescence dries room environmental Kuznets Curves：Humidity requires the section that ＜ 40%, temperature are in 25~28 DEG C within doors；(5) pollen release and drying：The polliniferous release of institute is completed in 2~4 days；The pollen released is naturally done drying in 12 hours；(6) pollen collecting：Dry frame is moved out dry room, is placed in calm room；(7) pollen preserves：4 DEG C of refrigerators are put into preserve.Using the inflorescence of present invention processing Alnus cremastogyne, the rate of drying of inflorescence can be accelerated；The aridity of pollen is obtained compared with high, water content is few, long term storage will not there is a phenomenon where lump and go mouldy.
Description
Technical field
The present invention relates to inflorescence is dry and pollen collecting method more particularly to a kind of Alnus cremastogyne male inflorescence rapid draing and
Pollen efficient collecting method.
Background technology
Alnus cremastogyne (Alnus cremastogyne Burli) is the arbor of Betulaceae Alder, and NATURAL DISTRIBUTION is in Sichuan
The ground such as basin and North Guizhou, Southern Shaanxi, the southeast of Gansu, meanwhile, also there is introducing a fine variety for large area on the ground such as Hunan and Fujian
Cultivation.Alnus cremastogyne growth is rapid, and well developed root system and energy fixed nitrogen, hygrophilous and barren-resistant, material is light and soft, and hardness is moderate, is excellent
Elegant short cycle solid wood Timber stands and green tree species.
Alnus cremastogyne monoecism, female flower and male flower are catkin, and female flower and male flower open in spring.Annual three
The first tenday period of a month in the middle of the month, male flower are developed at first, catkin overall elongated；Late March, when floral axis is elongated to ＞ 5cm, above floral axis
Anther is separated from each other, anther wall exposure in air, illumination it is sufficient, have gentle breeze when, anther wall gradually dehydration, color is by green
Turn yellow, subsequent anther wall dehydration is dry and cracked at faint yellow, meanwhile, it is female to take visible transparent or bean vermicelli color villus capitate stigma, female flower into
Entering can award the phase；After 1~2 day, the anther in the middle part of male inflorescence cracks at first, and after 2~3 days, the anther of male inflorescence upper and lower part is
Gradually crack, entire pollen acquisition time 4~7 days, in the middle latter stage of pollen acquisition time, floral axis also gradual dehydration atrophy.Pollen with
Wind descends slowly and lightly, and is captured by column cap, and spontaneous pollination process is completed.
Since the male and female florescence of same strain Alnus cremastogyne is overlapped, so self-pollination phenomenon is fairly common.Along with Sichuan
Terrain environment is complicated, and miniclimate numerous types, the alder flowering period in different microclimate environment is variant, causes differently
The flowering time of area's Alnus cremastogyne is not overlapped, and is hindered the gene exchange of different regions Alnus cremastogyne, is both easy to cause region
The formation of microspecies is also unfavorable for the selection of elite trees of Alnus cremastogyne Burk and the cultivation of breeding.Therefore, carry out the intraspecific hybridization work of Alnus cremastogyne
Make, is to expand Alnus cremastogyne hereditary basis, accelerates gene exchange in Alnus cremastogyne kind, cultivate excellent Alnus cremastogyne new varieties
Effective way.
Intraspecific hybridization needs to collect the Alnus cremastogyne male flower of different zones, using certain technical measures, pollen from flower
Simultaneously purification storage is released in medicine, and then the Alnus cremastogyne in other regions is hybridized using the pollen of collection.
The prior art will obtain single genotypical Alnus cremastogyne pollen, and acquisition is needed not discharge the male inflorescence of pollen, warp
After crossing certain processing, promote anther wall cracking and pollen release.
Existing techniqueflow：
Inflorescence is collected：The male inflorescence for acquiring 4~5 centimeter lengths, male inflorescence is placed sequentially in above newspaper or blank sheet of paper, inflorescence
Between be separated by 2~3 centimetres, so that inflorescence is come into full contact with air, put in the environment of shady and cool ventilation, as under in the shade windowsill.
Inflorescence is dried：Inflorescence is dried by the way of natural air drying, and wind speed is gentle breeze or slight air-flow, and use is forbidden to wear
Hall wind and electric fan, otherwise pollen can be blown winged, after 7~10 days, part anther wall cracking, pollen falls to newspaper and white naturally
On paper, in the drying process, floral axis dehydration and atrophy, using 3~5 days, anther all withered here become taupe with floral axis.
Pollen collecting：The residue for removing inflorescence on paper and being blended in pollen, leave behind the pollen of yellow in newspaper and
Paper surface；Foldable newspaper or blank sheet of paper pour into pollen in ox-hide big envelope made of paper, meanwhile, with the totally dry small scraps of paper
The pollen being sticky on paper is scraped, is collected into big envelope together.
Pollen preserves：The seam for big envelope being opened wide 1~2 centimeter length was placed in shady and cool ventilative environment, by 4~7 days
It is ventilative, the steam in pollen is fully dissipated, after sealing, big envelope is put into valve bag and is sealed, valve bag is placed on 4 DEG C
It is spare in refrigerator.
The shortcomings that preceding method is：
1, anther slow drying speed.Since the too fast flowing of worry about air leads to dispersing for pollen, using what is dried in the shade naturally
When the prior art is handled, inflorescence dehydrating speed is slow, at least needs 7 days time anther walls that can just crack, and nature
Lower pollen release time will be only 1~2 day.In the case that clearance space is small between the more inflorescence of inflorescence number, inflorescence drying
When will be longer.
2, the anther ratio that can be cracked is low, and pollen burst size is few.Under natural conditions, the season cracking period of anther compares floral axis
The period of dehydration atrophy is 3~5 days early, this shows after most of anther dehiscence releases pollen, and floral axis just starts atrophy change
Color.And existing processing mode can extend anther drying time, and but floral axis dehydration atrophy speed is constant, when inflorescence becomes in short-term, place
Close-packed arrays state is returned again in the anther of discrete state.After 3~5 days, here anther becomes taupe if integrally withering, in anther
Gradually dehydration rupture is dead for pollen.A small amount of anther at position, which can crack, only among inflorescence discharges pollen.
3, it is easy when pollen preserves hardened.The pollen that the prior art is collected is easy to absorb in air in shady and cool environment
Moisture, when leading to Cord blood, pollen is easy hardened blocking, and mildew is grown when serious.
Invention content
The present invention is to provide a kind of Alnus cremastogyne male inflorescence rapid draing in order to solve above-mentioned deficiency and pollen is efficiently received
Set method.
The above-mentioned purpose of the present invention is realized by technical solution below：A kind of Alnus cremastogyne male inflorescence rapid draing and
Pollen efficient collecting method, it is characterised in that：Include the following steps：
(1) inflorescence is collected：Acquire the male inflorescence of 4~5 centimeter length of Alnus cremastogyne；
(2) assembling of inflorescence drying frame：
Inflorescence drying frame is made of frame structure, pollen collecting layer, inflorescence abeyance layer, sealant.Frame structure is long 55
Centimetre, 42 centimetres wide, high 5.5 centimetres of 304 stainless steel frameworks, frame structure by a diameter of 2.5 millimeters of stainless steel strip it is parallel or
Vertical welding forms, 3.5 centimetres of stainless steel strip spacing；Pollen collecting layer is white parchment, is laid in stainless steel framework, sheep
Mulberry paper surface area is slightly larger than the tiling area of stainless steel framework, it is desirable that can be completely covered by stainless steel frame bottom and the vertical frame at four sides
Frame；Inflorescence abeyance layer can both be replaced by pollen collecting layer, and the nylon of one layer of 40~120 mesh can be also set up above stainless steel framework
Sieve, four side of nylon screen use specification to fix nylon mesh for the long-tail ticket holder of 15mm than 2~3 centimetres of four length of side of stainless steel framework
Net surrounding makes integrally 1~3 centimetre lower than stainless steel framework upper edge of nylon screen；Sealant is made of two layers of newspaper, and newspaper is laid in
Inflorescence dries the top of frame, and the long-tail ticket holder for being 15mm with specification, which is fixed, washes, and 2~3 long side 3~4, broadside specifications are
The long-tail ticket holder of 15mm.
According to whether carrying nylon screen, inflorescence can be dried frame and be divided into A types (without nylon screen) and Type B (band Buddhist nun
Imperial sieve).
(3) inflorescence is put：
The male inflorescence of collected 4~5 centimeter length is laid in A types to dry on the parchment of frame, without sequentially putting successively
It sets, 0.5~1 centimetre is spaced between inflorescence.Then newspaper is covered, four sides are clipped.Assembled inflorescence drying frame is put into drying
It is dried within doors.
In addition to this, collected male inflorescence can also be laid in above the nylon screen of Type B drying frame, without successively
Sequence is placed, and inflorescence, which can be overlapped, puts 2 layers.Then newspaper is covered, four sides are clipped.Assembled inflorescence drying frame is put into dry room
Inside it is dried.
(4) inflorescence dries room environmental Kuznets Curves：
Humidity requires the section that ＜ 40%, temperature are in 25~28 DEG C within doors.Temperature and humidity adjustment is carried out using air-conditioning, it is necessary
When can additional electrical oil heater auxiliary heating.
Dry frame is put：Dry frame is both placed on the table top of the level such as shelf, desk.It is not directly folded between dry frame
It puts, when being placed using shelf, distance is no less than 20 centimetres between dry frame up and down.A types dry under frame four angles can padded 2~
5 centimetres, accelerate air flowing.Type B drying frame can be directly placed on table top.Inflorescence dries frame can be by the wind of 30 DEG C of temperature ＜
Flow blow-through.
(5) pollen release and drying：
After placement in 12 hours, it can be found that the anther dehiscence at part inflorescence centre position, pollen fall to sheepskin
On paper.With the increase of drying time, it is seen that the anther whole dehydration above inflorescence darkens, contacted with parchment or with
The variation unobvious of the anther and other anther of nylon screen contact, show that the anther on the same inflorescence is synchronous mistake
Water, the inflorescence of this and inflorescence and conventional process under natural conditions after dehydration, gradually extend toward both ends since intermediate position
Mode is compared, and the polliniferous release of institute can be completed in 2~4 days, and wherein A types drying frame can complete pollen release work for 2~3 days
Industry, Type B drying frame can complete pollen release operation for 3~4 days.Meanwhile after pollen releases, inflorescence axis just starts to wither
Contracting, will not have an impact the release of anther.
12 hours interior energies of pollen that this method releases are naturally done drying, and A types dry dry gas stream, the Type B of frame bottom
The drying newspaper of dry arch can quickly dissipate the steam of pollen.
(6) pollen collecting：
Dry frame is moved out dry room, is placed in calm room.
A types are dried the inflorescence in frame and can be added with tweezers in diameter 30cm, the stainless steel sieve of mesh number 80 or nylon mesh, lead to
Slight reciprocating motion is crossed, is trembled in dry frame glutinous pollen is glued on inflorescence；Parchment one end is taken out out of dry frame, is delayed
Slow tilting 30 °, with finger or tweezers gently tap anther fragment, drying the impurity bottom such as scale parchment, by 2~3
After secondary percussion, impurity all can tumble or be slipped to parchment bottom, and pollen is then maintained at original position.After removing impurity, from
Parchment and doubling both ends are taken out in stainless steel framework, parchment are slightly firmly tapped, pollen from the parchment both sides of doubling
Abate, pollen are pooled to parchment center, open one section of parchment, and place 15~50mL plastic centrifuge tubes or ox in exit
The big envelope of mulberry paper tilts parchment, and pollen slides into centrifuge tube or big envelope, completes the collection of pollen.Pollen in centrifuge tube
Volume is no more than the 50% of centrifugation pipe volume, and pollen volume is no more than 15mL in big envelope.
The pollen that inflorescence releases in Type B drying frame can penetrate nylon screen, fall on the sheepskin paper bag of bottom, together
When, inflorescence and impurity after drying stay in nylon screen, remove inflorescence and impurity, remove nylon screen.Frame is dried using same A types
Pollen is collected in identical operation.
(7) pollen preserves：
The plastic centrifuge tube for having collected pollen can be directly placed into 4 DEG C of refrigerators, and modeling need to be put into containing polliniferous envelope
It in material bag, and is added with after the drier of 2~5 times of pollen volumes and tying sealing, 4 DEG C of refrigerators can be put into and preserved.Centrifuge tube
In pollen can persistently keep dry state 12~24 months in 4 DEG C of environment, after the renaturation that absorbs water, vigor pollen ratio can
Up to 80%~95%.Pollen in envelope can persistently keep dry state 10~18 months in 4 DEG C of environment, by the renaturation that absorbs water
Afterwards, 60%~85% pollen can rejuvenate.
Using the present invention processing Alnus cremastogyne inflorescence, the rate of drying of inflorescence can be accelerated, promote anther wall cracking and
The release of pollen.The time that pollen starts release was foreshortened to 2~3 days by 7~10 days of the prior art, when the sustained release of pollen
Between foreshortened to 1~2 day by 3~5 days of the prior art.
85%~95% anther energy season cracking and pollen is discharged after being handled using the present invention, on inflorescence.
The present invention obtains the aridity of pollen compared with high, water content is few, and long term storage will not there is a phenomenon where lump and go mouldy.
Compared with the prior art, the present invention has the advantages of：
1, due to the male inflorescence that handles of the present invention during drying by high-temperature control appropriate (25~28 DEG C) and
Humidity regulation improves the temperature and flow velocity of dry frame ambient air, reaches and accelerates inflorescence drying, especially the mesh of anther drying
's.Anther wall cracking discharges completely to pollen, and 5~7 days are needed under natural conditions, and conventional method needs 10~15 days, the present invention
Only need 3~4 days.Pollen is harvested in advance, it is meant that more Alnus cremastogynes can be given to carry out within florescence current year of Alnus cremastogyne
Hybridization pollination.
2, the Alnus cremastogyne male inflorescence of same quality or quantity is handled, the pollen that the present invention obtains is than conventional process hero
The pollen quality or quantity that inflorescence obtains are 5~8 times more.Reason, which is the anther above inflorescence, can complete season cracking and dissipate
Go out pollen, and the male inflorescence of conventional process can crack only less than 20% and release pollen.
3, the pollen that obtains of the present invention is dry and cracked and its when on parchment in anther, has begun to drying, therefore,
When pollen preserves, pollen, which has been dried, to be finished, and pollen independence is high, will not stick together by long-term preservation, non-
Often be conducive to pollen preservation and its vigor is kept.
4, pollen and impurity separation of the present invention is easy to operate efficient.When collecting, since impurity quality is much larger than
Pollen particles, by simply tilting and tapping the parchment in A types drying frame, you can impurity is all rolled down to sheepskin paper substrate
Portion.And frame is dried for Type B, due to there is the isolation of nylon screen, discharge the process of pollen in male inflorescence drying and anther dehiscence
In, that is, it has been automatically performed the separation of pollen and impurity.
5, due to mutual indepedent between pollen drying frame, and it is confined space in pollen frame, air flowing is slow, spends
Powder will not be blown afloat by air-flow and propagate in other pollen drying frame, and therefore, the present invention can focus on multiple genotypes simultaneously
Pollen, and can effectively prevent the appearance of cross contamination.
Specific implementation mode
The present invention is described in further detail with reference to embodiment.
A kind of Alnus cremastogyne male inflorescence rapid draing of the present invention and pollen efficient collecting method, it is characterised in that：Including
Following steps：
(1) inflorescence is collected：Acquire the male inflorescence of 4~5 centimeter length of Alnus cremastogyne；
(2) assembling of inflorescence drying frame：
Inflorescence drying frame is made of frame structure, pollen collecting layer, inflorescence abeyance layer, sealant.Frame structure is long 55
Centimetre, 42 centimetres wide, high 5.5 centimetres of 304 stainless steel frameworks, frame structure by a diameter of 2.5 millimeters of stainless steel strip it is parallel or
Vertical welding forms, 3.5 centimetres of stainless steel strip spacing；Pollen collecting layer is white parchment, is laid in stainless steel framework, sheep
Mulberry paper surface area is slightly larger than the tiling area of stainless steel framework, it is desirable that can be completely covered by stainless steel frame bottom and the vertical frame at four sides
Frame；Inflorescence abeyance layer can both be replaced by pollen collecting layer, and the nylon of one layer of 40~120 mesh can be also set up above stainless steel framework
Sieve, four side of nylon screen use specification to fix nylon mesh for the long-tail ticket holder of 15mm than 2~3 centimetres of four length of side of stainless steel framework
Net surrounding makes integrally 1~3 centimetre lower than stainless steel framework upper edge of nylon screen；Sealant is made of two layers of newspaper, and newspaper is laid in
Inflorescence dries the top of frame, and the long-tail ticket holder for being 15mm with specification, which is fixed, washes, and 2~3 long side 3~4, broadside specifications are
The long-tail ticket holder of 15mm.
According to whether carrying nylon screen, inflorescence can be dried frame and be divided into A types (without nylon screen) and Type B (band Buddhist nun
Imperial sieve).
(3) inflorescence is put：
The male inflorescence of collected 4~5 centimeter length is laid in A types to dry on the parchment of frame, without sequentially putting successively
It sets, 0.5~1 centimetre is spaced between inflorescence.Then newspaper is covered, four sides are clipped.Assembled inflorescence drying frame is put into drying
It is dried within doors.
In addition to this, collected male inflorescence can also be laid in above the nylon screen of Type B drying frame, without successively
Sequence is placed, and inflorescence, which can be overlapped, puts 2 layers.Then newspaper is covered, four sides are clipped.Assembled inflorescence drying frame is put into dry room
Inside it is dried.
(4) inflorescence dries room environmental Kuznets Curves：
Humidity requires the section that ＜ 40%, temperature are in 25~28 DEG C within doors.Temperature and humidity adjustment is carried out using air-conditioning, it is necessary
When can additional electrical oil heater auxiliary heating.
Dry frame is put：Dry frame is both placed on the table top of the level such as shelf, desk.It is not directly folded between dry frame
It puts, when being placed using shelf, distance is no less than 20 centimetres between dry frame up and down.A types dry under frame four angles can padded 2~
5 centimetres, accelerate air flowing.Type B drying frame can be directly placed on table top.Inflorescence dries frame can be by the wind of 30 DEG C of temperature ＜
Flow blow-through.
(5) pollen release and drying：
After placement in 12 hours, it can be found that the anther dehiscence at part inflorescence centre position, pollen fall to sheepskin
On paper.With the increase of drying time, it is seen that the anther whole dehydration above inflorescence darkens, contacted with parchment or with
The variation unobvious of the anther and other anther of nylon screen contact, show that the anther on the same inflorescence is synchronous mistake
Water, the inflorescence of this and inflorescence and conventional process under natural conditions after dehydration, gradually extend toward both ends since intermediate position
Mode is compared, and the polliniferous release of institute can be completed in 2~4 days, and wherein A types drying frame can complete pollen release work for 2~3 days
Industry, Type B drying frame can complete pollen release operation for 3~4 days.Meanwhile after pollen releases, inflorescence axis just starts to wither
Contracting, will not have an impact the release of anther.
12 hours interior energies of pollen that this method releases are naturally done drying, and A types dry dry gas stream, the Type B of frame bottom
The drying newspaper of dry arch can quickly dissipate the steam of pollen.
(6) pollen collecting：
Dry frame is moved out dry room, is placed in calm room.
A types are dried the inflorescence in frame and can be added with tweezers in diameter 30cm, the stainless steel sieve of mesh number 80 or nylon mesh, lead to
Slight reciprocating motion is crossed, is trembled in dry frame glutinous pollen is glued on inflorescence；Parchment one end is taken out out of dry frame, is delayed
Slow tilting 30 °, with finger or tweezers gently tap anther fragment, drying the impurity bottom such as scale parchment, by 2~3
After secondary percussion, impurity all can tumble or be slipped to parchment bottom, and pollen is then maintained at original position.After removing impurity, from
Parchment and doubling both ends are taken out in stainless steel framework, parchment are slightly firmly tapped, pollen from the parchment both sides of doubling
Abate, pollen are pooled to parchment center, open one section of parchment, and place 15~50mL plastic centrifuge tubes or ox in exit
The big envelope of mulberry paper tilts parchment, and pollen slides into centrifuge tube or big envelope, completes the collection of pollen.Pollen in centrifuge tube
Volume is no more than the 50% of centrifugation pipe volume, and pollen volume is no more than 15mL in big envelope.
The pollen that inflorescence releases in Type B drying frame can penetrate nylon screen, fall on the sheepskin paper bag of bottom, together
When, inflorescence and impurity after drying stay in nylon screen, remove inflorescence and impurity, remove nylon screen.Frame is dried using same A types
Pollen is collected in identical operation.
(7) pollen preserves：
The plastic centrifuge tube for having collected pollen can be directly placed into 4 DEG C of refrigerators, and modeling need to be put into containing polliniferous envelope
It in material bag, and is added with after the drier of 2~5 times of pollen volumes and tying sealing, 4 DEG C of refrigerators can be put into and preserved.Centrifuge tube
In pollen can persistently keep dry state 12~24 months in 4 DEG C of environment, after the renaturation that absorbs water, vigor pollen ratio can
Up to 80%~95%.Pollen in envelope can persistently keep dry state 10~18 months in 4 DEG C of environment, by the renaturation that absorbs water
Afterwards, 60%~85% pollen can rejuvenate.
Example the above is only the implementation of the present invention is not intended to limit the scope of the invention, every to utilize this hair
Equivalent structure or equivalent flow shift made by bright specification and embodiment content, it is relevant to be applied directly or indirectly in other
Technical field is included within the scope of the present invention.
Claims (1)
1. a kind of Alnus cremastogyne male inflorescence rapid draing and pollen efficient collecting method, it is characterised in that：Include the following steps：
(1) inflorescence is collected：Acquire the male inflorescence of 4~5 centimeter length of Alnus cremastogyne；
(2) assembling of inflorescence drying frame：
Inflorescence drying frame is made of frame structure, pollen collecting layer, inflorescence abeyance layer, sealant；
The frame structure is 55 centimetres long, 42 centimetres wide, high 5.5 centimetres of 304 stainless steel frameworks, and frame structure is by a diameter of
2.5 millimeters of stainless steel strip is parallel or vertical welding forms, 3.5 centimetres of stainless steel strip spacing；
The pollen collecting layer is white parchment, is laid in stainless steel framework, parchment surface area requirements can be completely covered by
The vertical frame of stainless steel frame bottom and four sides；
The inflorescence abeyance layer is replaced by pollen collecting layer, alternatively, setting up the Buddhist nun of one layer of 40~120 mesh above stainless steel framework
Imperial sieve, four side of nylon screen use specification to fix nylon for the long-tail ticket holder of 15mm than 2~3 centimetres of four length of side of stainless steel framework
Sieve surrounding makes integrally 1~3 centimetre lower than stainless steel framework upper edge of nylon screen；
The sealant is made of two layers of newspaper, and newspaper is laid in the top of inflorescence drying frame, the long-tail ticket for being 15mm with specification
It is clamping to wash surely, long side 3~4, the long-tail ticket holder that 2~3 specifications of broadside are 15mm；
According to whether carrying nylon screen, inflorescence drying frame is divided into without the A types drying frame of nylon screen and with the B of nylon screen
Type dries frame；
(3) inflorescence is put：
The male inflorescence of collected 4~5 centimeter length is laid in A types to dry on the parchment of frame, is placed without sequence successively, flower
0.5~1 centimetre is spaced between sequence；Then newspaper is covered, four sides are clipped；Assembled inflorescence drying frame be put into it is dry within doors into
Row drying；
(4) inflorescence dries room environmental Kuznets Curves：
Humidity requires the section that ＜ 40%, temperature are in 25~28 DEG C within doors；Temperature and humidity adjustment is carried out using air-conditioning, it is attached when necessary
Power up oil heater auxiliary heating；
Dry frame is put：Dry frame is both placed on the table top of the level such as shelf, desk；It is not directly stacked between dry frame,
When being placed using shelf, distance is no less than 20 centimetres between dry frame up and down；A types dry under four angles of frame padded 2~5 lis
Rice accelerates air flowing；Type B drying frame is directly placed on table top；Inflorescence dries frame can be by the distinguished and admirable straight of 30 DEG C of temperature ＜
It blows；
(5) pollen release and drying：
After placement in 12 hours, the anther dehiscence at position, pollen are fallen on parchment among the inflorescence of part；With drying
The increase of time, the anther whole dehydration above inflorescence, darkens, the anther for contacting with parchment or being contacted with nylon screen
With the variation unobvious of other anther, show that the anther on the same inflorescence is synchronous dehydration, this under natural conditions
Inflorescence and the inflorescence of conventional process after dehydration, are gradually compared toward the mode of both ends extension since intermediate position, can be at 2~4 days
The polliniferous release of interior completion institute, wherein A types drying frame can complete pollen release operation for 2~3 days, and Type B dries frame 3~4 days can
It completes pollen and discharges operation；Meanwhile after pollen releases, inflorescence axis just starts atrophy, will not be produced to the release of anther
It is raw to influence；
The pollen released is naturally done drying in 12 hours, i.e.,：A types dry the dry gas stream of frame bottom, Type B dries arch
Drying newspaper the steam of pollen is dissipated；
(6) pollen collecting：Dry frame is moved out dry room, is placed in calm room；
A types are dried the tweezers of the inflorescence in frame and are added in diameter 30cm, the stainless steel sieve of mesh number 80 or nylon mesh, by slight
It moves back and forth, is trembled in dry frame glutinous pollen is glued on inflorescence；Parchment one end is taken out out of dry frame, it is slowly tilting
30 °, with finger or tweezers gently tap anther fragment, drying the impurity bottom such as scale parchment, tapped by 2~3 times
Afterwards, impurity all can tumble or be slipped to parchment bottom, and pollen is then maintained at original position；After removing impurity, from stainless steel
Parchment and doubling both ends are taken out in frame, slightly firmly tap parchment, pollen from abate on the parchment both sides of doubling, are spent
Powder is pooled to parchment center, opens one section of parchment, and big in exit placement 15~50mL plastic centrifuge tubes or brown paper
Envelope tilts parchment, and pollen slides into centrifuge tube or big envelope, completes the collection of pollen；Pollen volume is not in centrifuge tube
More than the 50% of centrifugation pipe volume, pollen volume is no more than 15mL in big envelope；
The pollen that inflorescence releases in Type B drying frame can penetrate nylon screen, fall on the sheepskin paper bag of bottom, meanwhile, it does
Inflorescence and impurity after dry stay in nylon screen, remove inflorescence and impurity, remove nylon screen；It is complete using same A types drying frame
Pollen is collected in identical operation；
(7) pollen preserves：
The plastic centrifuge tube for having collected pollen is directly placed into 4 DEG C of refrigerators, and need to be put into polybag containing polliniferous envelope, and
It is added with after the drier of 2~5 times of pollen volumes and tying sealing, 4 DEG C of refrigerators can be put into and preserved；Pollen in centrifuge tube exists
Can persistently keep dry state 12~24 months in 4 DEG C of environment, after the renaturation that absorbs water, vigor pollen ratio up to 80%~
95%；Pollen in envelope can persistently keep dry state 10~18 months in 4 DEG C of environment, after the renaturation that absorbs water, 60%
~85% pollen can rejuvenate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610633075.2A CN106212267B (en) | 2016-08-04 | 2016-08-04 | A kind of Alnus cremastogyne male inflorescence rapid draing and pollen efficient collecting method |
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