CN104206265B - Willow liquid hybridization pollinating method - Google Patents
Willow liquid hybridization pollinating method Download PDFInfo
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- CN104206265B CN104206265B CN201410394598.7A CN201410394598A CN104206265B CN 104206265 B CN104206265 B CN 104206265B CN 201410394598 A CN201410394598 A CN 201410394598A CN 104206265 B CN104206265 B CN 104206265B
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- nutrient solution
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Abstract
The invention discloses a kind of willow liquid hybridization pollinating method, comprise the step such as preparation, pollination of the collection of pollen, willow Special flower powder liquid nutrient solution.The object that the present invention plays and saves cost, improve pollination efficiency, can save cost of labor 65%, and pollination efficiency improves 37%.
Description
Technical field
The present invention relates to a kind of willow liquid hybridization pollinating method.
Background technology
The method that existing hybridization of willows pollinating method mainly adopts artificial point to award, the operated in accordance with conventional methods time is long, and recruitment is many, and production cost is higher.And liquid pollination technology has, and pollination speed is fast, saving of labor, can keep the skin wet and nutrient to column cap rapidly, thus extend column cap pollination time.But liquid pollination technology is still immature, there is nutrient solution suspending power poor, pollen dispersion is uneven, and pollen is the problem such as easy swelling necrosis in nutrient solution.
Summary of the invention
The object of the present invention is to provide a kind of willow liquid hybridization pollinating method saved cost, improve pollination efficiency.
Technical solution of the present invention is:
A kind of willow liquid hybridization pollinating method, is characterized in that: its step is as follows:
(1) collection of pollen
Select the willow male flower of field large flower bud phase, 8 a.m. 30 timesharing gathers, adopt lower after, be laid on template, under 25 DEG C of dry ventilation conditions, naturally place diel, sieve by 80 mesh sieve after pollen is produced completely from flower pesticide, remove flower pesticide residue, pollen is placed in culture dish, then puts into drier, be placed on 0 ~ 5 DEG C of refrigerator for subsequent use;
(2) preparation of willow Special flower powder liquid nutrient solution
First starch Sodium Octenyl Succinate is cooled to room temperature after pollen dispersant fully dissolves with 60 DEG C of hot water, then willow Special flower powder liquid culture fluid is added, stirring makes it fully mix, add pollen again, the concentration of pollen is 0.6g/L, stirs, pollen is uniformly dispersed in the solution, obtain willow Special flower powder liquid nutrient solution, wherein the mass concentration of starch Sodium Octenyl Succinate is 0.2%; Described willow Special flower powder liquid culture fluid has the one-tenth of following weight percents to be grouped into: 0.006% Chelating state boron, 0.002% Chelating state zinc, 0.003% Chelating state calcium, 10% sucrose, all the other are water;
(3) pollinate
Select 30 points of-10 points the morning 9 time, when dew is just done, carry out sprinkling pollination to female flower open on withy, willow Special flower powder liquid nutrient solution is sprayed in 2 hours after preparation completes.
The shower nozzle spraying pollination employing is bell mouth shape, collar extension is many mesh type, and internal orifice diameter is 0.2cm, and collar extension diameter is 1.3cm, and mesh size is 0.03mm.
The object that the present invention plays and saves cost, improve pollination efficiency, can save cost of labor 65%, and pollination efficiency improves 37%.
Below in conjunction with embodiment, the invention will be further described.
Embodiment
A kind of willow liquid hybridization pollinating method, its step is as follows:
(1) collection of pollen
Select the willow male flower of field large flower bud phase, 8 a.m. 30 timesharing gathers, adopt lower after, be laid on template, under 25 DEG C of dry ventilation conditions, naturally place diel, sieve by 80 mesh sieve after pollen is produced completely from flower pesticide, remove flower pesticide residue, pollen is placed in culture dish, then puts into drier, be placed on 0 ~ 5 DEG C of refrigerator for subsequent use;
(2) preparation of willow Special flower powder liquid nutrient solution
First starch Sodium Octenyl Succinate is cooled to room temperature after pollen dispersant fully dissolves with 60 DEG C of hot water, then willow Special flower powder liquid culture fluid is added, stirring makes it fully mix, add pollen again, the concentration of pollen is 0.6g/L, stirs, pollen is uniformly dispersed in the solution, obtain willow Special flower powder liquid nutrient solution, wherein the mass concentration of starch Sodium Octenyl Succinate is 0.2%; Described willow Special flower powder liquid culture fluid has the one-tenth of following weight percents to be grouped into: 0.006% Chelating state boron, 0.002% Chelating state zinc, 0.003% Chelating state calcium, 10% sucrose, all the other are water;
(3) pollinate
Select 30 points of-10 points the morning 9 time, when dew is just done, carry out sprinkling pollination to female flower open on withy, willow Special flower powder liquid nutrient solution is sprayed in 2 hours after preparation completes.
The shower nozzle spraying pollination employing is bell mouth shape, collar extension is many mesh type, and internal orifice diameter is 0.2cm, and collar extension diameter is 1.3cm, and mesh size is 0.03mm.
Claims (1)
1. a willow liquid hybridization pollinating method, is characterized in that: its step is as follows:
(1) collection of pollen
Select the willow male flower of field large flower bud phase, 8 a.m. 30 timesharing gathers, adopt lower after, be laid on template, under 25 DEG C of dry ventilation conditions, naturally place diel, sieve by 80 mesh sieve after pollen is produced completely from flower pesticide, remove flower pesticide residue, pollen is placed in culture dish, then puts into drier, be placed on 0 ~ 5 DEG C of refrigerator for subsequent use;
(2) preparation of willow Special flower powder liquid nutrient solution
First starch Sodium Octenyl Succinate is cooled to room temperature after pollen dispersant fully dissolves with 60 DEG C of hot water, then willow Special flower powder liquid culture fluid is added, stirring makes it fully mix, add pollen again, the concentration of pollen is 0.6g/L, stirs, pollen is uniformly dispersed in the solution, obtain willow Special flower powder liquid nutrient solution, wherein the mass concentration of starch Sodium Octenyl Succinate is 0.2%; Described willow Special flower powder liquid culture fluid is grouped into by the one-tenth of following weight percents: 0.006% Chelating state boron, 0.002% Chelating state zinc, 0.003% Chelating state calcium, 10% sucrose, all the other are water;
(3) pollinate
Select 30 points of-10 points the morning 9 time, when dew is just done, carry out sprinkling pollination to female flower open on withy, willow Special flower powder liquid nutrient solution is sprayed in 2 hours after preparation completes;
The shower nozzle spraying pollination employing is bell mouth shape, collar extension is many mesh type, and internal orifice diameter is 0.2cm, and collar extension diameter is 1.3cm, and mesh size is 0.03mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410394598.7A CN104206265B (en) | 2014-08-13 | 2014-08-13 | Willow liquid hybridization pollinating method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410394598.7A CN104206265B (en) | 2014-08-13 | 2014-08-13 | Willow liquid hybridization pollinating method |
Publications (2)
| Publication Number | Publication Date |
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| CN104206265A CN104206265A (en) | 2014-12-17 |
| CN104206265B true CN104206265B (en) | 2016-02-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410394598.7A Active CN104206265B (en) | 2014-08-13 | 2014-08-13 | Willow liquid hybridization pollinating method |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106212267B (en) * | 2016-08-04 | 2018-09-14 | 四川省林业科学研究院 | A kind of Alnus cremastogyne male inflorescence rapid draing and pollen efficient collecting method |
| CN114223538A (en) * | 2021-12-31 | 2022-03-25 | 安徽农业大学 | Method for improving yield of soybean hybrid seed production |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1169237A (en) * | 1996-06-29 | 1998-01-07 | 邵阳市科学技术委员会 | Long-shaped rice hybrid seads production technology suitable to mechanized pollen-collection and pollination |
| CN1813521A (en) * | 2005-09-28 | 2006-08-09 | 河南科技学院 | Pollen liquid utilizing method for cotton hybrid seeds production |
| CN102204510B (en) * | 2011-05-25 | 2012-11-28 | 江苏沿江地区农业科学研究所 | Method for promoting hybridization of willows |
| CN102318549B (en) * | 2011-08-16 | 2012-07-25 | 南京农业大学 | Method for saving cost and improving efficiency for liquid pollination of pear tree |
| CN102805030B (en) * | 2012-08-03 | 2016-08-24 | 侯金波 | Bamboo willow crossbreeding technology |
| CN102771300A (en) * | 2012-08-21 | 2012-11-14 | 侯金波 | Willow breeding technology |
| CN103642748A (en) * | 2013-10-27 | 2014-03-19 | 大连东芳果菜专业合作社 | Liquid pollen for accurate pollination |
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