CN101637148A

CN101637148A - Mirid ovum collecting and hatching method

Info

Publication number: CN101637148A
Application number: CN200910066007A
Authority: CN
Inventors: 封洪强; 陈培育; 付晓伟; 蔡晓明; 李国平; 邱峰
Original assignee: Henan Academy of Agricultural Sciences
Current assignee: Henan Academy of Agricultural Sciences
Priority date: 2009-09-01
Filing date: 2009-09-01
Publication date: 2010-02-03

Abstract

The invention discloses a mirid ovum collecting and hatching method. A mirid ovum collecting tool comprises a culture dish convenient to disinfect, at least four layers of filter paper, and a flocculeused for adjusting the humidity of the filter paper and the culture dish, which are commonly used in laboratories; the floccule can be cellucotton, toilet paper rolls, sponge dough or fiber dough; the bottom of the culture dish is provided with at least four layers of filter paper, and the floccule is arranged in the central position of the filter paper; taking the floccule as the center, a plurality of convex marks are folded in the radial pattern around the filter paper; in order to ensure the humidity of the filter paper, the height of the floccule is slightly bigger than the depth of theculture dish bottom so as to ensure full contact with the inner wall of the culture dish cover. The invention can effectively collect mirid ovum, causes the ovum to be easily observed and effectivelyensures the ovum hatching rate.

Description

A kind of Mirid ovum collecting and hatching m

Technical field

The present invention relates to a kind of Mirid ovum collecting and hatching m, can collect ovum that the fleahopper silk worm produced in large quantities so that observation experiment, and guarantee its incubation rate.

Background technology

In recent years, the fleahopper silk worm became a kind of main pest gradually, and was extremely urgent to the research of fleahopper silk worm.The main plantation kidney bean that adopts is raised the fleahopper silk worm in the laboratory at present, makes the fleahopper silk worm ovum be originated in the tissue of kidney bean; Because the preservation of kidney bean hatching loaded down with trivial details or that preserve the product confrontation ovum of improper and kidney bean itself all has certain influence, and ovum embeds and is difficult in the tissue observing, and is an individual difficult point so relate to the research of ovum always.

Summary of the invention

Collect the loaded down with trivial details and difficult deficiency of observing of ovum of preservation that ovum caused in order to overcome kidney bean, the invention provides a kind of gathering unit, it is convenient that this device is not only preserved, and make ovum be easy to observation, made things convenient for the research work of being engaged in related fields.

The technical solution adopted for the present invention to solve the technical problems is: described Mirid ovum collecting apparatus comprises the cover laboratory culture dish of being convenient to sterilize commonly used, and described culture dish can be glass, plastics, pottery or metal; At least four layers filter paper; A floc sedimentation of regulating filter paper and culture dish humidity, described floc sedimentation can be cotton-wool, health spitball, sponge group or coma.It is characterized in that: the bottom at the bottom of the culture dish is equipped with at least four layers filter paper, and the center of filter paper is equipped with floc sedimentation; Considering that the fleahopper silk worm likes ovum is produced spawning habit on coarse uneven medium, concentrate for ease of the ovum grain, is the center with the floc sedimentation, the radial some roads convex epirelief that folds of ring filter paper.For guaranteeing the humidity in the culture dish, its port adds end cap, and the height of described floc sedimentation is slightly larger than the degree of depth of culture dish, guarantees to contact with the inwall of end cap.

Described collection method is following step: 1) bottom at the bottom of the culture dish is put at least four metafiltration paper and is used distilled water wetting, does not have water droplet to drip with culture dish instead and is as the criterion; 2) culture dish is put into the ovum grain that the box of raising the fleahopper silk worm is collected the fleahopper silk worm, collecting finishes adds water to again that filter paper suctions moisture content but till not dripping; 3) then floc sedimentation is placed on filter paper central authorities tops, covers culture dish or end cap gets final product.4) collection is had the said apparatus of Mirid ovum place under the suitable temp. and humidity and hatch, described temp. and humidity is meant: 26 ℃ of temperature, humidity 80 ± 10%RH, illumination L: D=16: 8; Wherein, the size of floc sedimentation will be accomplished not only to touch the inwall of top culture dish or end cap but also be guaranteed that up and down the slit is little between two culture dishes.When the water evaporates on the filter paper will be sucked back by floc sedimentation during to the inwall of top culture dish or end cap, thereby guarantee the humidity of filter paper, make the hatching process of ovum be in stable environment, and ovum produce and to be easy to observe on filter paper, for relevant research work provides a great convenience.

The invention has the beneficial effects as follows: can when collect the Mirid ovum grain effectively, make ovum be easy to observe, and guarantee the incubation rate of ovum effectively.

Description of drawings

Fig. 1 is the schematic diagram of concrete structure of the present invention; Fig. 2 is an A-A cutaway view shown in Figure 1.

Embodiment

The present invention is further described below in conjunction with drawings and Examples; Embodiment one, embodiment two are preferred implementation.

Embodiment one, as shown in Figure 1 and Figure 2, a kind of Mirid ovum collecting of the present invention and collection method, described Mirid ovum collecting apparatus comprise at the bottom of the culture dish 2, at least four layers filter paper 1, end cap 4, floc sedimentation 5, and described floc sedimentation can be cotton-wool, health spitball, sponge group or coma.It is characterized in that: 2 bottom is equipped with at least four layers filter paper 1 at the bottom of the culture dish, and the center of filter paper 1 is equipped with floc sedimentation 5; Considering that the fleahopper silk worm likes ovum is produced spawning habit on coarse uneven medium, concentrate for ease of the ovum grain, is the center with floc sedimentation 5, the ring filter paper 1 radial some roads convex epirelief 3 that folds.For guaranteeing at the bottom of the culture dish humidity in 2, its port adds end cap 4, and the height of described floc sedimentation 5 is slightly larger than at the bottom of the culture dish 2 the degree of depth, guarantees to contact with the inwall of end cap 4.

Embodiment two, do not change other apparatus described in the embodiment one, substitute end cap 4 with 2 stacks at the bottom of the cover culture dish of same specification, also can realize purpose of the present invention.

Described collection method is following step:

1) 2 bottoms are put at least four metafiltration paper 1 and are used distilled water wetting till do not drip at the bottom of the culture dish;

2) collect the ovum grain of fleahopper silk worm with 2 usefulness conventional methods at the bottom of the culture dish that step 1) is obtained, collecting finishes adds water to again that filter paper 1 suctions moisture content but till not dripping;

3) wetting till do not drip floc sedimentation 5 adding distil waters then, be placed on filter paper 1 central authorities top, cover culture dish lid 4;

4) collection is had the above-mentioned apparatus of Mirid ovum place under the suitable temp. and humidity and hatch, described temp. and humidity is meant: 26 ℃ of temperature, humidity 80 ± 10%RH, illumination L: D=16: 8.

Claims

1. Mirid ovum collecting and hatching m, described Mirid ovum collecting and hatching utensil comprise (2) and a culture dish lid (4) at the bottom of the culture dish, at least four layers filter paper (1), floc sedimentation (5), it is characterized in that: (2) bottom is equipped with at least four layers filter paper (1) at the bottom of the culture dish, and the center of filter paper (1) is equipped with floc sedimentation (5); The height of floc sedimentation (5) greater than culture dish at the bottom of the degree of depth of (2), guarantee that the inwall that covers (4) with culture dish fully contacts; With floc sedimentation (5) is the center, the radial some roads convex epireliefs (3) that fold of ring filter paper (1).

2. according to described a kind of Mirid ovum collecting of claim 1 and hatching m, it is characterized in that: described collection and hatching m are following step:

1) (2) bottom is put at least four metafiltration paper (1) and is used distilled water wetting till do not drip at the bottom of the culture dish;

2) collect the ovum grain of fleahopper silk worm with (2) at the bottom of the culture dish that step 1) is obtained with conventional method, collecting finishes adds water to again that filter paper (1) suctions moisture content but till not dripping;

3) wetting till do not drip floc sedimentation (5) adding distil water then, be placed on filter paper (1) central authorities top, cover culture dish lid (4);