CN102487896A - Device for observing development of predatory mite individuals with nematodes as food and preparation and use methods thereof - Google Patents
Device for observing development of predatory mite individuals with nematodes as food and preparation and use methods thereof Download PDFInfo
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- CN102487896A CN102487896A CN2011103857696A CN201110385769A CN102487896A CN 102487896 A CN102487896 A CN 102487896A CN 2011103857696 A CN2011103857696 A CN 2011103857696A CN 201110385769 A CN201110385769 A CN 201110385769A CN 102487896 A CN102487896 A CN 102487896A
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- centrifuge tube
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Abstract
The invention discloses a device for observing development of predatory mite individuals with nematodes as food and preparation and use methods thereof. The device comprises a culture dish, a centrifuge tube cap, a colloidal flat plate and a black sticker, wherein the centrifuge tube cap is arranged inside the culture dish; the black sticker is stuck to the top surface of the centrifuge tube cap and is inversely stuck to and fixed at the bottom of the culture dish by a double faced adhesive tape; and the colloidal flat plate is installed inside the centrifuge tube cap and is prepared from agarose and deionized water in a ratio of 1:50. The device has the following advantages and effects: the device has the advantages of good air permeability and concentrated range of observation and can fully guarantee the humidity needed by growth and development of predatory mites and effectively prevent escape and loss of predatory mites; and the survival time of nematodes can be lengthened, so that the growth and development and reproduction conditions of predatory mites with nematodes as food can be continuously observed, thus improving the accuracy and scientificity of the observation data.
Description
Technical field
The present invention relates to the plant pest management field, particularly a kind of is the ontogenetic finder of predation mite and preparation method thereof and the method for using of food with the Plant nematode.
Background technology
Plant nematode is one of plant infectious pathogen, influences growth and development of plants, causes plant products minimizing, quality to descend, even dead and total crop failure.At present; To the most frequently used method of preventing and treating of plant nematode is that the chemical control method is promptly used chemical pesticide; But the chemical control method not only costs an arm and a leg, cost high, and it has, and height is malicious, the characteristic of high residue, can cause serious environmental to pollute and many other harmful effects.
Adopt predation acarid control plant nematode; Compare with traditional chemical agent control; The predation mite can not produce poisoning, residual and problem of environment pollution caused; It is thus clear that the predation mite is that a kind of utmost point has the plant nematode of using value to prevent and treat resource, correlation technique and the technology of research and utilization predation mite controlling plant nematode seem particularly important.But the ontogenetic finder of existing predation mite; Gas permeability is relatively poor, and humidity is low, and is relatively poor to the living environment simulation of predation mite; Simultaneously; Exist field of view excessive, the predation mite problem such as lose that is easy to escape is unfavorable for to the predation mite being that the ontogeny situation of food is carried out long-term tracing observation and research with the nematode.
Summary of the invention
The objective of the invention is to overcome the shortcoming that exists in the prior art, provide that a kind of technology is simple, the predation mite of easy operating with the nematode is the ontogenetic finder of food.
Another object of the present invention is to provide the preparation method of above-mentioned finder.
A purpose more of the present invention is to provide the method for using of above-mentioned finder.
The object of the invention is realized through following technical proposals:
A kind ofly prey on the ontogenetic finder that mite is food with the nematode, comprise culture dish 1, centrifuge tube lid 2, colloid dull and stereotyped 3 and black paster 4; Centrifuge tube lid 2 is positioned at the inside of culture dish 1, and the end face of centrifuge tube lid 2 posts black paster 4 and is inverted the bottom that culture dish 1 is fixed in stickup through double faced adhesive tape; Colloid dull and stereotyped 3 is equipped with in the inside of centrifuge tube lid 2.
Said colloid dull and stereotyped 3 is made up of agarose and deionized water.
Said predation mite is the preparation method of the ontogenetic finder of food with the nematode, comprises the steps:
(1) according to agarose: the proportioning of deionized water=1: 50, earlier agarose is contained in the container, add deionized water and mix; Be heated to agarose then and be dissolved in water formation glue fully; Pour glue into centrifuge tube lid 2 again, condensation obtains colloid dull and stereotyped 3;
(2) end face at the centrifuge tube lid 2 that colloid dull and stereotyped 3 is housed sticks black paster 4, is inverted the bottom that sticks on culture dish 1 with double faced adhesive tape again;
(3) add water to 1/2 high place of centrifuge tube lid in culture dish 1 the inside, form the boom platform, preparing the predation mite at last is the ontogenetic finder of food with the nematode.
Said predation mite is the method for using of the ontogenetic finder of food with the nematode, comprises the steps:
(1) nematode is added in the distilled water, place in the centrifuge tube with the centrifugal 2min of the speed of 4000r/min; Inhale with suction pipe then and remove supernatant, stay the liquid that contains nematode in the centrifuge tube bottom;
The fluid drips that (2) will contain nematode and is smeared evenly on colloid dull and stereotyped 3, leaves standstill 10～20min, makes the surface moisture evaporation;
(3) collect the ovum that the predation mite is produced in 4h, and will prey on single the placing on the colloid dull and stereotyped 3 of ovum of mite;
(4) will load nematode and place the dark culturing case to cultivate with the finder of preying on mite, condition of culture is set to 25 ± 1 ℃, 80%RH; Every 12h takes out from incubator, under stereomicroscope, observes growing and survival of predation mite, the preoviposition period of record, egg-laying period, post-egg stage, day egg laying amount and life-span.
The present invention compared with prior art has following advantage and effect:
(1) good permeability of finder of the present invention, range of observation are concentrated, and can fully ensure the predation mite required humidity of growing, and can prevent effectively that the escape of preying on mite from losing.
(2) it is dull and stereotyped that the present invention adopts the colloid of agarose, compares with the plain agar flat board, can make the time of nematode survival longer, can be growing of food and the breeding situation is observed constantly with the nematode to the predation mite, improves the accuracy and the science of observed data.
Description of drawings
Fig. 1 is the structural representation of the ontogenetic finder of food with the nematode for the predation mite.
Fig. 2 is the structure schematic top plan view of the ontogenetic finder of food with the nematode for the predation mite.
Wherein, 1, culture dish; 2, centrifuge tube lid; 3, colloid is dull and stereotyped; 4, black paster.
Embodiment
Below in conjunction with embodiment the present invention is done further detailed description, but embodiment of the present invention is not limited thereto.
The ontogenetic finder of predation mite comprises culture dish 1, centrifuge tube lid 2, colloid dull and stereotyped 3 and black paster 4.The diameter of centrifuge tube lid 2 is 10mm, the high 5mm of being, is positioned at the inside of culture dish 1.The end face of centrifuge tube lid 2 posts black paster 4, and is inverted the bottom that culture dish 1 is fixed in stickup through double faced adhesive tape.The inside of centrifuge tube lid 2 has colloid dull and stereotyped 3, and colloid dull and stereotyped 3 is made up of agarose and deionized water, and colloid dull and stereotyped 3 is used to place nematode and preys on mite.
The preparation method of the ontogenetic finder of above-mentioned predation mite comprises the steps:
(1) according to agarose: the proportioning of deionized water=1: 50, earlier agarose is contained in the triangular flask, add deionized water and mix; Be heated to agarose with micro-wave oven then and be dissolved in water formation glue fully; Pour glue into the centrifuge tube lid to about 1/2 of its volume again, condensation 10min obtains colloid dull and stereotyped 3;
(2) stick the black paster 4 of round diameter 10mm at the end face of the centrifuge tube lid 2 that colloid dull and stereotyped 3 is housed, be inverted the centre that sticks on culture dish 1 bottom with double faced adhesive tape again;
(3) add water to centrifuge tube in culture dish 1 the inside and cover 2 high 1/2 places, form the boom platform, preparing the predation mite at last is the ontogenetic finder of food with the nematode.
The method for using of the ontogenetic finder of above-mentioned predation mite comprises the steps: that (1) adds nematode in the distilled water, places in the 1.5mL centrifuge tube with the centrifugal 2min of the speed of 4000r/min; Inhale with suction pipe then and remove supernatant, stay the liquid that about 0.1mL contains nematode in the centrifuge tube bottom;
(2) draw the fluid drips that contains nematode stay on colloid dull and stereotyped 3 with suction pipe, and smear evenly, leave standstill 10～20min, make the evaporation of surperficial moisture content appropriateness;
(3) collect the ovum that the predation mite is produced in less than 4h, and with single the placing on the colloid flat board 3 of ovum of choosing the predation mite that pin will collect;
(4) will load nematode and place the dark culturing case to cultivate with the observation device of preying on mite, condition of culture is set to 25 ± 1 ℃, 80%RH; Every 12h takes out from incubator, under stereomicroscope, observes growing and survival of predation mite, the preoviposition period of record, egg-laying period, post-egg stage, day egg laying amount and life-span.
Claims (4)
1. prey on the finder of growing that mite is food with the nematode for one kind, it is characterized in that: comprise culture dish, centrifuge tube lid, colloid flat board and black paster; The centrifuge tube lid is positioned at the inside of culture dish, and the end face of centrifuge tube lid posts the black paster and is inverted the bottom that culture dish is fixed in stickup through double faced adhesive tape; The colloid flat board is equipped with in the inside of centrifuge tube lid.
2. predation mite according to claim 1 is the ontogenetic finder of food with the nematode, and it is characterized in that: said colloid flat board is made up of agarose and deionized water.
3. prey on the preparation method that mite is the ontogenetic finder of food with the nematode for one kind, it is characterized in that comprising the steps:
(1) according to agarose: the proportioning of deionized water=1: 50, earlier agarose is contained in the container, add deionized water and mix; Be heated to agarose then and be dissolved in water formation glue fully; Pour glue into the centrifuge tube lid again, condensation obtains the colloid flat board;
(2) stick the black paster at the end face that the dull and stereotyped centrifuge tube lid of colloid is housed, be inverted the bottom that sticks on culture dish with double faced adhesive tape again;
(3) add water to 1/2 high place of centrifuge tube lid in the culture dish the inside, form the boom platform, prepare the ontogenetic finder of predation mite at last.
4. prey on the method for using that mite is the ontogenetic finder of food with the nematode for one kind, it is characterized in that comprising the steps:
(1) nematode is added in the distilled water, place in the centrifuge tube with the centrifugal 2min of the speed of 4000r/min; Inhale with suction pipe then and remove supernatant, stay the liquid that contains nematode in the centrifuge tube bottom;
The fluid drips that (2) will contain nematode and is smeared evenly on the colloid flat board, leaves standstill 10～20min, makes surperficial water evaporates;
(3) collect the ovum that the predation mite is produced in 4h, and will prey on single the placing on the colloid flat board of ovum of mite;
(4) will load nematode and place dark incubator to cultivate with the observation device of preying on mite, condition of culture is set to 25 ± 1 ℃, 80%RH; Every 12h takes out from incubator, under stereomicroscope, observes growing and survival of predation mite, the preoviposition period of record, egg-laying period, post-egg stage, day egg laying amount and life-span.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110385769 CN102487896B (en) | 2011-11-28 | 2011-11-28 | Device for observing development of predatory mite individuals with nematodes as food and preparation and use methods thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110385769 CN102487896B (en) | 2011-11-28 | 2011-11-28 | Device for observing development of predatory mite individuals with nematodes as food and preparation and use methods thereof |
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| CN102487896A true CN102487896A (en) | 2012-06-13 |
| CN102487896B CN102487896B (en) | 2013-09-11 |
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| CN 201110385769 Expired - Fee Related CN102487896B (en) | 2011-11-28 | 2011-11-28 | Device for observing development of predatory mite individuals with nematodes as food and preparation and use methods thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872081A (en) * | 2015-06-08 | 2015-09-02 | 于丽辰 | Feeding box for mites and small insects and application of feeding box |
| CN105154325A (en) * | 2015-09-24 | 2015-12-16 | 中国科学院东北地理与农业生态研究所 | Fungus parasitic soybean heterodera glycines ichinohe detecting device |
| CN109221026A (en) * | 2018-11-20 | 2019-01-18 | 山东农业大学 | A kind of efficient device and method for collecting Bradysia odoriphaga ovum |
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| US20110277042A1 (en) * | 2004-12-31 | 2011-11-10 | Karel Jozef Florent Bolckmans | Mite Composition, Use Thereof, Method for Rearing a Phytoseiid Predatory Mite, Rearing System for Rearing Said Phytoseiid Predatory Mite and Methods for Biological Pest Control on a Crop |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872081A (en) * | 2015-06-08 | 2015-09-02 | 于丽辰 | Feeding box for mites and small insects and application of feeding box |
| CN104872081B (en) * | 2015-06-08 | 2018-05-01 | 于丽辰 | It is a kind of to be used for mite class and the raising box of small insects and its application |
| CN105154325A (en) * | 2015-09-24 | 2015-12-16 | 中国科学院东北地理与农业生态研究所 | Fungus parasitic soybean heterodera glycines ichinohe detecting device |
| CN105154325B (en) * | 2015-09-24 | 2017-06-13 | 中国科学院东北地理与农业生态研究所 | Fungi autoeciousness soybean cyst nematode Heterodera glycines detection means |
| CN109221026A (en) * | 2018-11-20 | 2019-01-18 | 山东农业大学 | A kind of efficient device and method for collecting Bradysia odoriphaga ovum |
| CN109221026B (en) * | 2018-11-20 | 2023-09-22 | 山东农业大学 | Device and method for efficiently collecting slow-eye mushroom mosquito eggs of Chinese chives |
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| CN102487896B (en) | 2013-09-11 |
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