CN102021131B - Bacillus licheniformis strain and application thereof - Google Patents
Bacillus licheniformis strain and application thereof Download PDFInfo
- Publication number
- CN102021131B CN102021131B CN2010105475371A CN201010547537A CN102021131B CN 102021131 B CN102021131 B CN 102021131B CN 2010105475371 A CN2010105475371 A CN 2010105475371A CN 201010547537 A CN201010547537 A CN 201010547537A CN 102021131 B CN102021131 B CN 102021131B
- Authority
- CN
- China
- Prior art keywords
- fjat
- bacillus strain
- fermented liquid
- watermelon
- bacillus licheniformis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a Bacillus licheniformis strain FJAT-4 with very good biological control effect, which is separated and screened from rhizosphere soil of crops, i.e. tomatoes, watermelons, cowpeas, and the like of six counties (cities) of Yongtai, Fuqing, Minhou, and the like in Fuzhou, Fujian Province in China. The invention also relates to fermentation liquor capable of preventing and curing watermelon fusarium wilt, which is prepared by adopting the Bacillus licheniformis strain FJAT-4. The invention provides the Bacillus licheniformis strain FJAT-4; and in addition, the fermentation liquor prepared by adopting the Bacillus licheniformis strain FJAT-4 has higher inhibiting effect on watermelon fusarium oxysporum and exerts very good effect on preventing and curing the watermelon fusarium wilt in the fields, thereby having better production and application prospects.
Description
[technical field]
The present invention relates to a kind of mikrobe and application thereof, relate in particular to a kind of lichem bacillus strain FJAT-4 and use the fermented liquid that its preparation prevents and treats watermelon blight.
[background technology]
Watermelon blight is the soil-borne vascular bundle disease due to being infected by Fusarium oxysporum watermelon specialized form (Fusarium oxysporum f.sp.niveum).Blight is the severe diseases that causes harm on the watermelon, and greater loss is all caused in each watermelon producing region in the world.Because this disease dependent territory passes disease, germ generally is distributed in the dark arable layer of 15～30cm, mainly survives the winter in soil with mycelium, chlamydospore; And the 5～6a of can surviving, control is difficulty very, and the general sickness rate of second crop soil is more than 30%; Serious plot reaches more than 80%, even causes total crop failure.Plant is one of disease that seriously restricts watermelon production in case morbidity promptly is difficult to cure.In recent years, China's cultivating watermelon area is increasing, and continuous cropping watermelon area also increases thereupon, and therefore, the generation of watermelon blight also is on the rise.
Control to the former bacterium of watermelon blight (watermelon Fusarium oxysporum); Still mainly rely on chemical pesticide at present; This not only brings problems such as pesticide residue, environmental pollution; And pathogenic bacteria is developed immunity to drugs, bring bigger difficulty to preventing and controlling. for seeking prophylactico-therapeutic measures safely and effectively, the biological control of wilt more and more causes people's attention.Generally believe in the world that at present biological control is to solve one of approach the most likely of soil-borne disease; But also there is not to prevent and treat specially the biological control medicament of watermelon blight both at home and abroad; So those skilled in the art press for the new microbial preparation of effective control watermelon blight and the appearance of method.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of lichem bacillus strain (Bacillus licheniformis) FJAT-4; This lichem bacillus strain FJAT-4 is preserved in Chinese typical culture collection center (CCTCC) on November 1st, 2011; The address is Lopa Nationality an ancient woman's ornament mountain, a Wuhan City Wuhan University; Deposit number is CCTCC NO:M 2010285, and adopts this bacterial strain to prepare a kind of fermented liquid that can effectively prevent and treat watermelon blight.
The present invention solves the problems of the technologies described above through following technical scheme:
Separate from the rhizosphere soil of crops such as the tomato in 6 counties such as Yongtai, Fuzhou City, Fujian Province, Fuqing, Minhou (city), watermelon, cowpea in this laboratory and screening obtains having the bacterial strain of fine biological control effect; And will carry out determination and analysis to the physicochemical property of this bacterial strain, identify that finally it is the bacterial strain of Bacillus licheniformis.
Further, adopt this bacterial strain to prepare a kind of fermented liquid that can prevent and treat watermelon blight, the concrete steps of its preparation are:
(1) activation of described lichem bacillus strain FJAT-4: with transfering loop described lichem bacillus strain FJAT-4 is lined on the NA substratum, and in constant incubator, cultivate 48h, culture temperature is set at 30 ℃;
(2) preparation of seed liquor: single colony inoculation of the lichem bacillus strain FJAT-4 that step (1) is obtained and is placed on constant temperature shaking table shaking culture in seed culture medium, rotating speed 200rpm, and temperature is set at 30 ℃, promptly gets seed liquor after cultivating 24h;
(3) preparation of fermentation liquid: the seed liquor of the lichem bacillus strain FJAT-4 that step (2) is obtained is inoculated in the sterilized fermention medium, inoculum size 1%, and stir speed (S.S.) 200rpm, air flow 1: 1.2, temperature is set at 30 ℃; In the process of fermentation culture, get an amount of fermented liquid at set intervals and measure its cell concentration, when the cell concentration value of measuring greater than 2.0 * 10
9During CFU/mL, then obtain required fermented liquid;
Wherein, the component of NA substratum is in the said step (1): beef extract 0.1%, and peptone 0.5%, yeast extract paste 0.5%, sodium-chlor 0.5%, agar 1.8% is with water preparation, pH 7.0-7.2; The middle fermention medium of seed culture medium and step (3) is the NB substratum in the said step (2), and its component is: beef extract 0.1%, and peptone 0.5%, yeast extract paste 0.5%, sodium-chlor 0.5% is with water preparation, pH 7.0-7.2; And the per-cent in each nutrient media components is weight ratio.
Beneficial effect of the present invention is: a kind of lichem bacillus strain (Bacillus licheniformis) FJAT-4 is provided; And adopt this lichem bacillus strain FJAT-4 preparation can play the fermented liquid of preventive and therapeutic effect to watermelon blight; This fermented liquid has very strong restraining effect to the Fusarium oxysporum that causes watermelon blight, can finely play the effect of control watermelon blight in the field.
[embodiment]
A kind of lichem bacillus strain of the present invention (Bacillus licheniformis) FJAT-4 separates the rhizosphere soil of crop such as the tomato, watermelon, cowpea from 6 counties such as Yongtai, Fuzhou City, Fujian Province, Fuqing, Minhou (city) and screening obtains having the bacterial strain of fine biological control effect, and adopts this bacterial strain to prepare a kind of fermented liquid that can prevent and treat watermelon blight.
1. the separation of bacterial strain:
(1) get rhizosphere soil sample 10g in the 90mL sterilized water, fully vibration back absorption 1mL soil supension carries out gradient dilution;
(2) the soil diluent that step (1) is obtained is coated on the NA culture medium flat plate, the NA culture medium flat plate is placed in 30 ℃ of constant incubators cultivate 72h then;
(3) single bacterium colony of first picking step (2) cultivation acquisition carries out smear; Magenta dyeing back microscopy; Choose the bacterial strain that can produce gemma and change slant medium over to and preserve, as bacterial strain to be measured, then with inoculation to be measured in the BPY substratum; And this BPY substratum placed rotating speed 200rpm, 30 ℃ of following shaking culture 72h, collect nutrient solution; The component of BPY substratum wherein: peptone 1.0%, beef extract 0.5%, YE 0.3%, glucose 0.5%, NaCl 0.5%, and with the zero(ppm) water preparation, pH 7.2; In addition, the per-cent in this BPY nutrient media components is weight ratio.
(4) be indicator with the watermelon Fusarium oxysporum, adopt the bacterial strain to be measured in the nutrient solution that cup-plate method collects step (3) to screen concrete operations: with the dull and stereotyped cultivation of PDA watermelon Fusarium oxysporum, to collect spore with sterilized water behind the 7d, process spore suspension; The spore suspension for preparing is joined temperature reduce in about 50 ℃ of molten state PDA substratum, pour in the petridish behind the mixing rapidly; After treating that liquid in the petridish solidifies; Disposed tubes dish on the flat board of band pathogenic bacteria spore; Get the collected nutrient solution of step (3) 200 μ m in the pipe dish, observe behind the cultivation 48h down at 30 ℃, and compare with sterilized water; Measure antibacterial circle diameter through the diameter interior extrapolation, thereby filter out the inhibited bacterial strain of pathogenic bacteria spore.
2. the evaluation of bacterial strain:
With what obtained the pathogenic bacteria spore had the mensuration that the strongest inhibiting bacterial strain carries out physics and chemistry and biological characteristics; The main form and the biological property that obtain this bacterial strain are following: bacteria colony white, shriveling, opaque; Gramstaining is positive, and the nourishing body cell is shaft-like; Gemma is arranged, and gemma is closely oval, and middle life or inferior end are given birth to, and do not expand.Below be its concrete Physiology and biochemistry identification of indicator:
Annotate :+show effect is arranged or to respond;-show no effect or reactionless
The in full accord of each index and Bacillus licheniformis (Bacillus licheniformis) found in above-mentioned each index back of analysing and comparing, judge that then this bacterial strain is Bacillus licheniformis (Bacillus licheniformis).
3. the concrete preparation process that can prevent and treat the fermented liquid of watermelon blight is:
(1) activation of lichem bacillus strain FJAT-4: with transfering loop lichem bacillus strain FJAT-4 is lined on the NA substratum of bacterial strain, and in constant incubator, cultivate 48h, and culture temperature is set at 30 ℃;
(2) preparation of seed liquor: single colony inoculation (bottled amount is the 25mL/250mL triangular flask) in the triangular flask that is loaded with seed culture medium of the lichem bacillus strain FJAT-4 that step (1) is obtained; And triangular flask placed constant temperature shaking table shaking culture; Rotating speed 200rpm; Temperature is set at 30 ℃, promptly gets seed liquor behind the cultivation 24h;
(3) preparation of fermentation liquid: the fermention medium of in fermentor tank, packing in advance; And at 121 ℃ of 30min that sterilize down; The seed liquor of following the lichem bacillus strain FJAT-4 that step (2) is obtained is inoculated in the fermentor tank inoculum size 1%, stir speed (S.S.) 200rpm; Air flow 1: 1.2, temperature are set at 30 ℃; And in the process of fermentation culture, get an amount of fermented liquid at set intervals and measure its cell concentration, when the cell concentration value of measuring greater than 2.0 * 10
9During CFU/mL (the about 48h of incubation time), then obtain required fermented liquid.
Wherein, the component of NA substratum is in the step (1): beef extract 0.1%, and peptone 0.5%, yeast extract paste 0.5%, sodium-chlor 0.5%, agar 1.8% is with water preparation, pH 7.0-7.2; The middle fermention medium of seed culture medium and step (3) is the NB substratum in the step (2), and its component is: beef extract 0.1%, and peptone 0.5%, yeast extract paste 0.5%, sodium-chlor 0.5% is with water preparation, pH 7.0-7.2; And the per-cent in each nutrient media components is weight ratio.
4. the fermented liquid that can prevent and treat watermelon blight is carried out the biological control test
Experiment one: different concns lichem bacillus strain FJAT-4 fermented liquid is to the dull and stereotyped antagonistic effect of withered germ of water-melon
(1) test materials
On flat board, cultivate in advance 7d pathogenic bacteria (watermelon Fusarium oxysporum).
(2) TP
Test group, blank group and positive controls are set, in test group, respectively get an amount of fermented liquid earlier and be diluted to 10,10
2... 10
6Doubly subsequent use, then the fermented liquid after fermented liquid stoste and each dilution is mixed with 1: 9 volume ratio with the PDA substratum that is cooled to 50 ℃ of molten states respectively mutually, shake up the back and fall dull and stereotyped; Being not the blank group with the flat board that the PDA substratum of 50 ℃ of molten states mixes down with the liquid nutrient medium of fermented liquid; Dislike the dull and stereotyped positive control group that mould clever 400 times of liquid and PDA substratum mix down with the medicament commonly used of preventing and treating watermelon blight in the market; After treating dull and stereotyped all condensations of each group; Insert a pathogenic bacteria bacterium sheet of cultivating the about 0.6cm of diameter of 7d in advance respectively in each dull and stereotyped central authorities; Then each flat board all is upside down in 26 ℃ of incubators and cultivates; After cultivating 4d, 6d, 8d, use " ten " word method of masurement to measure each dull and stereotyped colony diameter of going up pathogenic bacteria respectively, calculate inhibiting rate.Test group, blank group and positive controls all repeat 3 times.
(3) test-results
Adopt the DPS statistical software that the result of each group is carried out statistical study, its antagonism is the result see like following table 1.The result shows: the fermented liquid of lichem bacillus strain FJAT-4 is that the watermelon Fusarium oxysporum has very strong restraining effect to pathogenic bacteria; And the difference between the bacteriostasis rate of its stoste and different multiples diluent is not obvious; 8d after processing; The bacteriostasis rate of fermented liquid stoste is 68.1%, and dilutes 10,10
2, 10
3, 10
4The bacteriostasis rate of the fermented liquid doubly is all high than stoste, wherein dilutes 10
3Fermented liquid bacteriostasis rate doubly is the highest, reaches 80.9%, 10
5, 10
6The bacteriostasis rate of times diluent is little low, but still more than 50%, shows that fermented liquid has persistence to the restraining effect of watermelon Fusarium oxysporum, can not reduce As time goes on and rapidly; And in positive controls, medicament is disliked mould clever 400 times of liquid As time goes on the bacteriostasis rate of watermelon Fusarium oxysporum is then reduced, and handles back 4d, is 89.3% to the inhibiting rate of sickle-like bacteria, handles back 8d, and bacteriostasis rate is 78.7%, than biocontrol microorganisms fermented liquid 10
3The bacteriostasis rate (80.9%) of times diluent is low, so this fermented liquid may have more stable high preventive effect to the watermelon Fusarium oxysporum in the field.
The bacterial strain FJAT-4 fermented liquid of table 1 different concns is to the restraining effect of withered germ of water-melon
Annotate: 1. diameter * 100% of bacteriostasis rate=(diameter of the diameter-treatment group bacterium colony of contrast bacterium colony)/contrast bacterium colony
2. 8d after processing, the watermelon Fusarium oxysporum bacterium colony on the blank group flat board has covered with whole flat board, and plate diameter is 8.80cm
Test two: the fermented liquid of lichem bacillus strain FJAT-4 is to the field controling test of watermelon blight
(1) test materials
Experimental cultivar: early spring carbuncle.
(2) TP
The test place is located on plot that watermelon blight all seriously took place in recent years, Yongtai, Fujian; Treatment group and control group are set, all soak seed for two groups, seedling pan sowing next day on February 25th, 2009; During March 11; Treatment group is used to water the watermelon seedling in the seedling pan after fermented liquid stoste is diluted 50 times, and control group comprises three contrasts, respectively with the watermelon seedling in 600 times of liquid of tricyclazole, 800 times of liquid of dimension line gram and the clear water pouring seedling pan.March 24 transplanted two groups watermelon seedling respectively, and the watermelon seedling after the firm transplanting was irritated root respectively handle, and investigated the incidence of each watermelon seedling of field on April 7.
(3) test-results
As shown in table 2; Incidence investigation to the watermelon seedling shows; Irritate root with 50 times of diluents of fermented liquid, tricyclazole and three kinds of medicaments of dimension line gram and handle the watermelon seedling of just having transplanted, the mortality ratio of watermelon seedling is respectively 7.4%, 11.3% and 12.0% behind the 14d, all significantly is lower than 19.3% (P＜0.01) of clear water contrast; Wherein the correction preventive effect of fermented liquid is 61.6%, is higher than tricyclazole and 41.7% and 37.6% of dimension line gram.
Table 2 Bacillus licheniformis FJAT-4 control watermelon blight is respectively handled the mortality ratio of watermelon seedling
| Handle | Watermelon seedling sum (strain) | Death toll (strain) | Mortality ratio (%) | Control effect (%) |
| 50 times of liquid of fermented liquid | 81 | 6 | 7.4a* | 61.6 |
| 600 times of liquid of tricyclazole | 80 | 9 | 11.3ab | 41.7 |
| 800 times of liquid of dimension line gram | 83 | 10 | 12.0ab | 37.6 |
| Clear water | 83 | 16 | 19.3b | --- |
Annotate: 1.* representes do not have difference when the multiple comparisons p=0.05 of Duncan ' s multiple range test with the numeral of same letter;
2. control effect=(contrast diseased plant rate-processings diseased plant rate)/contrast diseased plant rate * 100%
To sum up; The fermented liquid that the present invention utilizes lichem bacillus strain (Bacillus licheniformis) FJAT-4 to prepare gained has stronger restraining effect to the watermelon Fusarium oxysporum; And when the field control watermelon blight, have good effect, promptly have production application prospect preferably.
Claims (2)
1. lichem bacillus strain; It is characterized in that: said bacterial strain is lichem bacillus strain (Bacillus licheniformis) FJAT-4; Be preserved in the Chinese typical culture collection center that is positioned at Lopa Nationality an ancient woman's ornament mountain, Wuhan City Wuhan University on November 1st, 2010, deposit number is CCTCC NO:M 2010285.
2. fermented liquid of preventing and treating watermelon blight, it is characterized in that: the concrete steps of its preparation method are:
(1) activation of the lichem bacillus strain FJAT-4 described in the claim 1: the lichem bacillus strain FJAT-4 described in the claim 1 is lined on the NA substratum with transfering loop; And in constant incubator, cultivate 48h, culture temperature is set at 30 ℃;
(2) preparation of seed liquor: single colony inoculation of the lichem bacillus strain FJAT-4 that step (1) is obtained and is placed on constant temperature shaking table shaking culture in seed culture medium, rotating speed 200rpm, and temperature is set at 30 ℃, promptly gets seed liquor after cultivating 24h;
(3) preparation of fermentation liquid: the seed liquor of the lichem bacillus strain FJAT-4 that step (2) is obtained is inoculated in the sterilized fermention medium, inoculum size 1%, and stir speed (S.S.) 200rpm, air flow 1: 1.2, temperature is set at 30 ℃; In the process of fermentation culture, get an amount of fermented liquid at set intervals and measure its cell concentration, when the cell concentration value of measuring greater than 2.0 * 10
9During CFU/mL, then obtain required fermented liquid;
Wherein, the component of NA substratum is in the said step (1): beef extract 0.1%, and peptone 0.5%, yeast extract paste 0.5%, sodium-chlor 0.5%, agar 1.8% is with water preparation, pH 7.0-7.2; The middle fermention medium of seed culture medium and step (3) is the NB substratum in the said step (2), and its component is: beef extract 0.1%, and peptone 0.5%, yeast extract paste 0.5%, sodium-chlor 0.5% is with water preparation, pH 7.0-7.2; And the per-cent in each nutrient media components is weight ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105475371A CN102021131B (en) | 2010-11-16 | 2010-11-16 | Bacillus licheniformis strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105475371A CN102021131B (en) | 2010-11-16 | 2010-11-16 | Bacillus licheniformis strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102021131A CN102021131A (en) | 2011-04-20 |
| CN102021131B true CN102021131B (en) | 2012-05-09 |
Family
ID=43862916
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105475371A Active CN102021131B (en) | 2010-11-16 | 2010-11-16 | Bacillus licheniformis strain and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102021131B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087541A (en) * | 2014-07-16 | 2014-10-08 | 南京新果园生态农业科技有限公司 | Biocontrol strain capable of preventing and controlling tomato early blight and application thereof |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159570A (en) * | 2011-12-12 | 2013-06-19 | 河北农业大学 | Solid microbial fertilizer and preparation method thereof |
| CN106801023B (en) * | 2016-12-31 | 2020-03-27 | 中国农业科学院农业资源与农业区划研究所 | Bacillus licheniformis Y16 and application thereof in preventing and treating crop soil-borne diseases |
| CN108157675A (en) * | 2017-12-18 | 2018-06-15 | 陈建峰 | A kind of crab feed and preparation method thereof |
| CN108396002B (en) * | 2018-03-07 | 2019-12-06 | 山东新禾丰作物营养有限公司 | Bacillus licheniformis and application thereof in preventing and treating sweet melon fusarium wilt |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186855A (en) * | 2007-11-09 | 2008-05-28 | 郑州明飞科技发展有限公司 | Special-purpose metal calcium core granular combustion-supporting desulfurization coke cleaning agent for bombardment type coal and preparation method thereof |
-
2010
- 2010-11-16 CN CN2010105475371A patent/CN102021131B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186855A (en) * | 2007-11-09 | 2008-05-28 | 郑州明飞科技发展有限公司 | Special-purpose metal calcium core granular combustion-supporting desulfurization coke cleaning agent for bombardment type coal and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘波等.农作物枯萎病的研究进展.《厦门大学学报(自然科学版)》.2004,第43卷第47-58页. * |
| 葛慈斌等.生防菌NH-BS-2000 对西瓜枯萎病原菌抑制作用的研究.《厦门大学学报(自然科学版)》.2004,第43卷第87-90页. * |
| 郑雪芳等.瓜类作物枯萎病生防菌BS-2000和JK-2的分子鉴定.《福建农业学报》.2006,第21卷(第2期),第154-157页. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087541A (en) * | 2014-07-16 | 2014-10-08 | 南京新果园生态农业科技有限公司 | Biocontrol strain capable of preventing and controlling tomato early blight and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102021131A (en) | 2011-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104928212B (en) | Bacillus megaterium X3 and preparation method thereof, application | |
| CN101575574B (en) | Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection | |
| CN101423812B (en) | Bacillus amyloliquefaciens and microbiological preparation and preparation method thereof | |
| CN105296381B (en) | One bacillus subtilis CYY-25 and its application | |
| CN102433281B (en) | Streptomyces katrae NB20, as well as culture method and application thereof | |
| CN101851597B (en) | Streptomyces griseoflavus for resisting alfalfa diseases and screening method thereof | |
| CN103396971B (en) | Burkholderia cepacia and application thereof | |
| CN102747020B (en) | Bacillus subtilis and application thereof | |
| CN106399178B (en) | Bacillus amyloliquefaciens and its application with degradation Phos and bacteriostasis | |
| CN114456986B (en) | Broad-spectrum antibacterial bacillus aryabhattai and application thereof | |
| CN105543132A (en) | Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases | |
| CN110272833A (en) | Trichoderma harzianum HL119 and its application | |
| CN103436457A (en) | Burkholderia cepacia, and cultivation method and application thereof | |
| CN102021131B (en) | Bacillus licheniformis strain and application thereof | |
| CN106834130A (en) | One plant of Strain of Beauveria bassiana and its application to grub larva highly pathogenicity | |
| CN101654665B (en) | Method for preparing Bacillus subtilis | |
| CN102168042A (en) | Pseudomonas aeruginosa and application thereof | |
| CN102329756A (en) | Streptomyces albospinus strain BWL15-4 for preventing and treating banana vascular wilt and application thereof | |
| CN102787084A (en) | Bacillus methylotrophicus 4-L-16 for prevention and control of banana vascular wilt and its application | |
| CN106434490A (en) | Ginseng bacterium TY15-2 with effects of disease prevention and growth promotion and application thereof | |
| CN105296364A (en) | Penicillium oxalicum, preparation method and application thereof | |
| CN101558766B (en) | Trichoderma solid granules for preventing and controlling tobacco soil-borne fungus diseases and preparation method thereof | |
| CN106399148A (en) | Kosakonia radicincitans and application thereof | |
| CN108384734A (en) | A kind of biocontrol agent and preparation method thereof of prevention tobacco rhizome class disease | |
| CN103146609B (en) | Pseudomonas fluorescens and method for preventing phytophthora capsici thereby |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230926 Address after: 530007, 1st to 5th floors, A-2, Nanning Biotechnology Center, No. 18 Xinji Road, Nanning City, Guangxi Zhuang Autonomous Region Patentee after: NANNING HARWORLD BIOTECHNOLOGY Co.,Ltd. Address before: 350000 No. 54, 247, Fuzhou, Fujian Patentee before: AGRICULTURAL BIORESOURCES INSTITUTE OF FUJIAN ACADEMY OF AGRICULTURAL SCIENCES |
|
| TR01 | Transfer of patent right |