CN108849755A - Oriental fruit months larva store method - Google Patents
Oriental fruit months larva store method Download PDFInfo
- Publication number
- CN108849755A CN108849755A CN201810591326.4A CN201810591326A CN108849755A CN 108849755 A CN108849755 A CN 108849755A CN 201810591326 A CN201810591326 A CN 201810591326A CN 108849755 A CN108849755 A CN 108849755A
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- larva
- gauze
- container
- preservation
- mature
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000004321 preservation Methods 0.000 claims abstract description 19
- 238000011161 development Methods 0.000 claims abstract description 17
- 230000018109 developmental process Effects 0.000 claims abstract description 17
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 9
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 9
- 210000004681 ovum Anatomy 0.000 claims abstract description 9
- 238000011081 inoculation Methods 0.000 claims abstract description 5
- 230000000384 rearing effect Effects 0.000 claims abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 241000382353 Pupa Species 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 3
- 238000013473 artificial intelligence Methods 0.000 claims description 3
- 230000009194 climbing Effects 0.000 claims description 3
- 230000032683 aging Effects 0.000 claims description 2
- 210000003608 fece Anatomy 0.000 claims description 2
- 230000017448 oviposition Effects 0.000 abstract description 5
- 241000607479 Yersinia pestis Species 0.000 abstract description 4
- 235000013601 eggs Nutrition 0.000 abstract description 3
- 230000012447 hatching Effects 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract 1
- 230000007774 longterm Effects 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000005868 ontogenesis Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of oriental fruit months larva store method, including feed preparation, inoculation, rearing conditions, mature larva, preservation larva is collected, pupates and sprout wings.Using oriental fruit months larva store method of the invention, storage life can be extended to 6 months, be 12 times of the prior art；Single head female adult pest eggs on average amount after preservation is 100, is 2.5 times of the prior art；Adult is 9.4 days without deformity, average life span, is the 2 times or more of the prior art.It is saved using the method for the present invention, mature larva is pupated, after emerging adult oviposition, and 75 μm of the diameter of ovum, 87% or more hatching rate, larvae development go through the phase 13 days, are not significantly different with the individual normally raised.It is saved using the method for the present invention and saves space, can be reserved for worm amount 100 between 1 lift-off, saving the more existing store method of cost reduces by 70% or more.It can guarantee that uninterruptedly providing enough test worms in the difficult winter-spring season of oriental fruit months raising is normally carried out experiment using the method for the present invention.
Description
Technical field
The invention belongs to technical field of plant protection, and in particular to a method of extend oriental fruit months retention cycle.
Background technique
Oriental fruit months is a kind of moth fruit pest, and in China, large area occurs and endangers.To study oriental fruit months row
For, physiology and Control Technology, need to carry out long-term raising and preservation indoors.During the feeding process, oriental fruit months is always
Practical life condition will appear kind after long-term continuously rearing there are larger difference in growth and development stage, with nature
The problems such as group degenerates.While the purpose in order to realize indoor population long-term preservation, it needs constantly to be raised, above-mentioned need of work
Expend a large amount of man power and material.Saving the common method of oriental fruit months at present is by cryo-conservation pupa, i.e., on 5 DEG C of left sides
The right side, holding time are 10-15 days.At the holding time 20 days, part pupa sprouts wings adult out as lopsided wing, adult average life span by
Be reduced within 10 days 7 days, egg laying amount by 115/it is female fall to 80/it is female；At the holding time 30 days, 70% or more pupa is sprouted wings out
Adult is deformity, and the average life span of adult further falls to 4 days, egg laying amount be reduced to 40/it is female, Life of Adult and egg laying amount are big
Width decline.Problem of the existing technology solves the problems, such as that oriental fruit months retention cycle is too short by saving pupa, to alleviation
Oriental fruit months quality and quantity decline, reduce cost of labor, feed and water power expend etc. effect it is little, it would be highly desirable to explore one kind
The method of oriental fruit months retention cycle can effectively be extended.
Summary of the invention
In view of the deficienciess of the prior art, the object of the present invention is to provide a kind of oriental fruit months larva store method,
This method can realize oriental fruit months demand that is efficient, easy, saving for a long time, to meet in research work to oriental fruit months
The requirement of quality and quantity.
To achieve the above object, present invention employs following technical solutions.
Oriental fruit months larva store method of the invention, includes the following steps：
（1）Feed prepare larvae feeds be fresh apple young fruit, young fruit diameter be 3-4cm, can now adopt, can also deepfreeze,
Pericarp wants smooth, corrugationless；
（2）Young fruit is put in raising container by inoculation, and the oriental fruit months ovum that will hatch gently is seeded on apple young fruit,
Per kilogram young fruit is inoculated with normal development ovum 200-250 head；
（3）Rearing conditions are placed in container is raised in temperature-adjustable, humidity and the artificial intelligence of illumination incubator, relative humidity
70%, temperature and photoperiod be：It is dark phase, 14 hours at 20 DEG C；Intensity of illumination at 25 DEG C is 3000-4000Lux, 10 hours；
（4）It collects after mature larva is inoculated with 12 days, the gauze after container sterilizes will be raised and wrapped up, to larvae development to old
It climbs out of when ripe larva from apple internal, when climbing to gauze, collects, select that weight 12mg or more, body colour be red, liveness
Low, undamaged mature larva is transferred in clean container；
（5）After placement of the preservation larva mature larva in clean container 2 days, intracorporal excreta has been emptied to mature larva,
Prepare the new gauze through high-temperature sterilization of another width, and the wet water of the libation at an ancient wedding ceremony, control gauze water content, gently will with writing brush in 15%-25%
Mature larva is swept on new gauze from original gauze, and density is every 100 square centimeters of placements 40-50 head, then aging
One layer of gauze through high-temperature sterilization is covered on larva, mature larva can slightly creep between two layers of gauze, after being evenly distributed, gently
It is put in through high-temperature sterilization, side in venthole, long 20-10,5-10 centimetres wide, high 8-10 centimetres of container, each container is put
Layer 2-3 larva is set, is closed the lid, places in the incubator of constant temperature and humidity and saves, storage temperature is 3-6 DEG C, relative humidity 70-
75%, it during preservation, observes at interval of 15 days opening containers, if any insect death or the mouldy situation of gauze, to choose in time dead
Worm, replacement gauze；
（5）It pupates and when emergence preservation larva needs to continue development, will be taken out from preservation condition equipped with the hold-up vessel of larva,
Gauze with larva is taken out, is replaced into the container through high-temperature sterilization, is put into 25 DEG C, under the conditions of relative humidity 70-75%,
Normal development is transferred to pupating to mature larva, and then pupa is taken out, is sprouted wings in the container being put under the same terms.
The present invention compared with prior art the advantages of：（1）Using oriental fruit months larva store method of the invention, can prolong
Long storage life was 12 times of the prior art to 6 months；Single head female adult pest eggs on average amount after preservation is 100, is existing skill
2.5 times of art；Adult is 9.4 days without deformity, average life span, is the 2 times or more of the prior art.
（2）It is saved using the method for the present invention, mature larva is pupated, after emerging adult oviposition, 75 μm of diameter, the hatching rate of ovum
87% or more, larvae development goes through the phase 13 days, is not significantly different with the individual normally raised.
（3）It is saved using the method for the present invention and saves space, can be reserved for worm amount 100 between 1 lift-off, it is more existing to save cost
Store method reduces by 70% or more.
（4）It is enough to can guarantee that the winter-spring season for raising difficulty in oriental fruit months uninterruptedly provides using the method for the present invention
Test worm is normally carried out experiment.
Specific embodiment
Oriental fruit months larva store method of the invention, includes the following steps：
（1）Feed prepare larvae feeds be fresh apple young fruit, young fruit diameter be 3-4cm, can now adopt, can also deepfreeze,
Pericarp wants smooth, corrugationless, and smooth, corrugationless young fruit is conducive to larva and effectively eats into fruit.
（2）Young fruit is put in raising container by inoculation, and the oriental fruit months ovum that will hatch gently is seeded in apple children
On fruit, per kilogram young fruit is inoculated with normal development ovum 200-250 head, and suitable inoculum density will guarantee that oriental fruit months has enough
Food and activity space, obtain that development is sufficient, the biggish individual of weight, be conducive to improve the utilization rate of young fruit again, reduce and raise
Form this.
（3）Rearing conditions are placed in container is raised in temperature-adjustable, humidity and the artificial intelligence of illumination incubator, relatively
Humidity 70%, temperature and photoperiod be：It is dark phase, 14 hours at 20 DEG C；Intensity of illumination at 25 DEG C is 3000-4000Lux, 10
Hour, the larval weight raised with this condition is most heavy, holding time longest.
（4）After collecting mature larva inoculation 12 days, the gauze after raising container disinfection is wrapped up, to larvae development
It climbs out of when to mature larva from apple internal, when climbing to gauze, collects, select weight 12mg or more, body colour red, active
It spends low, undamaged mature larva to be transferred in clean container, weeds out hypogenetic larva, improve the matter of preservation larva
Amount.
（5）After placement of the preservation larva mature larva in clean container 2 days, intracorporal excretion has been emptied to mature larva
Object prepares the new gauze through high-temperature sterilization of another width, and the wet water of the libation at an ancient wedding ceremony, controls gauze water content in 15%-25%, gently use writing brush
Mature larva is swept on new gauze from original gauze, density is every 100 square centimeters of placements 40-50 head, then old
One layer of gauze through high-temperature sterilization is covered on ripe larva, mature larva can slightly creep between two layers of gauze, after being evenly distributed,
Put down gently in through high-temperature sterilization, side in venthole, long 20-10,5-10 centimetres wide, high 8-10 centimetres of container, each container
Layer 2-3 larva is placed, is closed the lid, is placed in the incubator of constant temperature and humidity and save, storage temperature is 3-6 DEG C, relative humidity 70-
75%, it during preservation, observes at interval of 15 days opening containers, if any insect death or the mouldy situation of gauze, to choose in time dead
Worm, replacement gauze.Using this store method, storage life can extend to 6 months.
（5）Pupate and emergence preservation larva need continue development when, will equipped with larva hold-up vessel from preservation condition
It takes out, the gauze with larva is taken out, replace into the container through high-temperature sterilization, be put into 25 DEG C, relative humidity 70-75% item
Under part, normal development is transferred to pupating to mature larva, then pupa is taken out, is sprouted wings in the container being put under the same terms.At
Without deformity after worm emergence, average life span is 9.4 days；Single head female adult pest eggs on average amount after preservation is 100, the diameter 75 of ovum
μm, 87% or more hatching rate, larvae development goes through the phase 13 days, is not significantly different with the ontogeny normally raised.
Claims (1)
1. a kind of oriental fruit months larva store method, includes the following steps：
（1）Feed prepare larvae feeds be fresh apple young fruit, young fruit diameter be 3-4cm, can now adopt, can also deepfreeze,
Pericarp wants smooth, corrugationless；
（2）Young fruit is put in raising container by inoculation, and the oriental fruit months ovum that will hatch gently is seeded on apple young fruit,
Per kilogram young fruit is inoculated with normal development ovum 200-250 head；
（3）Rearing conditions are placed in container is raised in temperature-adjustable, humidity and the artificial intelligence of illumination incubator, relative humidity
70%, temperature and photoperiod be：It is dark phase, 14 hours at 20 DEG C；Intensity of illumination at 25 DEG C is 3000-4000Lux, 10 hours；
（4）It collects after mature larva is inoculated with 12 days, the gauze after container sterilizes will be raised and wrapped up, to larvae development to old
It climbs out of when ripe larva from apple internal, when climbing to gauze, collects, select that weight 12mg or more, body colour be red, liveness
Low, undamaged mature larva is transferred in clean container；
（5）After placement of the preservation larva mature larva in clean container 2 days, intracorporal excreta has been emptied to mature larva,
Prepare the new gauze through high-temperature sterilization of another width, and the wet water of the libation at an ancient wedding ceremony, control gauze water content, gently will with writing brush in 15%-25%
Mature larva is swept on new gauze from original gauze, and density is every 100 square centimeters of placements 40-50 head, then aging
One layer of gauze through high-temperature sterilization is covered on larva, mature larva can slightly creep between two layers of gauze, after being evenly distributed, gently
It is put in through high-temperature sterilization, side in venthole, long 20-10,5-10 centimetres wide, high 8-10 centimetres of container, each container is put
Layer 2-3 larva is set, is closed the lid, places in the incubator of constant temperature and humidity and saves, storage temperature is 3-6 DEG C, relative humidity 70-
75%, it during preservation, observes at interval of 15 days opening containers, if any insect death or the mouldy situation of gauze, to choose in time dead
Worm, replacement gauze；
（5）It pupates and when emergence preservation larva needs to continue development, will be taken out from preservation condition equipped with the hold-up vessel of larva,
Gauze with larva is taken out, is replaced into the container through high-temperature sterilization, is put into 25 DEG C, under the conditions of relative humidity 70-75%,
Normal development is transferred to pupating to mature larva, and then pupa is taken out, is sprouted wings in the container being put under the same terms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810591326.4A CN108849755A (en) | 2018-06-10 | 2018-06-10 | Oriental fruit months larva store method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810591326.4A CN108849755A (en) | 2018-06-10 | 2018-06-10 | Oriental fruit months larva store method |
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| Publication Number | Publication Date |
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| CN108849755A true CN108849755A (en) | 2018-11-23 |
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| CN201810591326.4A Pending CN108849755A (en) | 2018-06-10 | 2018-06-10 | Oriental fruit months larva store method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109984098A (en) * | 2019-05-15 | 2019-07-09 | 山西农业大学 | A kind of oriental fruit months experimental population expanding propagation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6138165B2 (en) * | 1980-06-23 | 1986-08-28 | Shinetsu Chem Ind Co | |
| BG51183A1 (en) * | 1991-03-20 | 1993-03-15 | Georgi Ja Papanov | Attractant compositition |
| CN101720724A (en) * | 2009-12-04 | 2010-06-09 | 沈阳农业大学 | Long-term storage method of bee species of artificially diapaused and induced Chouioia cunea |
| CN101731186A (en) * | 2010-02-08 | 2010-06-16 | 西北农林科技大学 | Artificial rearing method of Grapholitha molesta Busck |
| CN105746440A (en) * | 2016-03-10 | 2016-07-13 | 山西省农业科学院植物保护研究所 | Artificial feeding device and artificial feeding method for oriental fruit moth |
-
2018
- 2018-06-10 CN CN201810591326.4A patent/CN108849755A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6138165B2 (en) * | 1980-06-23 | 1986-08-28 | Shinetsu Chem Ind Co | |
| BG51183A1 (en) * | 1991-03-20 | 1993-03-15 | Georgi Ja Papanov | Attractant compositition |
| CN101720724A (en) * | 2009-12-04 | 2010-06-09 | 沈阳农业大学 | Long-term storage method of bee species of artificially diapaused and induced Chouioia cunea |
| CN101731186A (en) * | 2010-02-08 | 2010-06-16 | 西北农林科技大学 | Artificial rearing method of Grapholitha molesta Busck |
| CN105746440A (en) * | 2016-03-10 | 2016-07-13 | 山西省农业科学院植物保护研究所 | Artificial feeding device and artificial feeding method for oriental fruit moth |
Non-Patent Citations (2)
| Title |
|---|
| 杜娟: "梨小食心虫Grapholitha molesta （Busk）生物学特性及人工饲养技术研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 雷朝亮: "《昆虫资源学》", 31 December 2011, 武汉：湖北科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109984098A (en) * | 2019-05-15 | 2019-07-09 | 山西农业大学 | A kind of oriental fruit months experimental population expanding propagation method |
| CN109984098B (en) * | 2019-05-15 | 2022-03-04 | 山西农业大学 | Propagation method for experimental population of oriental fruit moth |
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Application publication date: 20181123 |