CN105766809B - A kind of small arthropod life table experimental method - Google Patents
A kind of small arthropod life table experimental method Download PDFInfo
- Publication number
- CN105766809B CN105766809B CN201610204968.5A CN201610204968A CN105766809B CN 105766809 B CN105766809 B CN 105766809B CN 201610204968 A CN201610204968 A CN 201610204968A CN 105766809 B CN105766809 B CN 105766809B
- Authority
- CN
- China
- Prior art keywords
- life table
- poly
- methyl methacrylate
- aperture
- plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000002474 experimental method Methods 0.000 title claims abstract description 23
- 241000238421 Arthropoda Species 0.000 title claims abstract description 9
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims abstract description 14
- 239000004926 polymethyl methacrylate Substances 0.000 claims abstract description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerol Substances OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 5
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Chinese gallotannin Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 abstract 1
- 238000002224 dissection Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 description 6
- 241000611866 Tyrophagus putrescentiae Species 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- 238000011982 device technology Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Instructional Devices (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of small arthropod life table experimental methods, its step is that 40 conical apertures are uniformly stamped on poly (methyl methacrylate) plate, pre-prepared man-made feeds are smeared inside aperture, single experimental subjects individual is put into inside aperture, covered and the smearing water glycerite around coverslip above aperture, poly (methyl methacrylate) plate is put into growth cabinet and is cultivated, in dissection microscopic observation.The present invention can conveniently and effectively carry out life table experiment.
Description
Technical field
The present invention relates to a kind of methods for testing (reproductive capacity experiment) for small arthropod life table, are particularly suitable for
In the life table experiment of the small insects such as mite class, the method that entire life table is tested can be conveniently and effectively completed.
Background technology
Commonly used in the implementation process of small arthropod life table experiment is film lid or culture dish conduct
Device is raised needed for experiment.And need largely to raise device in life table experimentation, single film lid or culture dish are made
It is taken up space by feeding device too big, and is not easy to experimenter's operation and observation, be unfavorable for the number of next step life table experiment
According to statistics and analyze.So in view of factors above, a kind of feeding device technology is urgently needed in life table experiment, reality can be accounted for
It is smaller to test space, and can effectively easily complete entire life table experiment in raising device.
Invention content
The purpose of the present invention is to provide one kind to be simple and efficient, and small and exquisite easily life table experimental method solves existing skill
It is generated using the time-consuming and laborious and acquisition and record to experimental data of tradition feeding device technology when carrying out life table experiment in art
Influence and eventually lead to the problem of result of study inaccuracy.
To achieve the above object, present invention employs following technical proposals.A kind of small arthropod life table experiment side
Method, technical step are as follows：
1. proportioned man-made feeds to be uniformly applied to the inside of poly (methyl methacrylate) plate aperture, naturally dry；
2. experimental subjects is chosen in aperture, covered smears 1 around coverslip：The water-glycerol of 1 proportioning is molten
Liquid keeps humidity in aperture；
3. carrying out the processing of step 1 and step 2 in each aperture of poly (methyl methacrylate) plate；
4. being put into poly (methyl methacrylate) plate to the artificial climate for setting (temperature of experiment setting, humidity, illumination)
In case；
5. the survival of observation experiment object and egg laying amount under anatomical lens daily record data.
Beneficial effects of the present invention
1. the present invention can conveniently and effectively carry out life table experiment, prior art inefficiency and time-consuming and laborious.
2. the present invention using poly (methyl methacrylate) plate as feeding device, it is small and exquisite portable, in growth cabinet the space occupied compared with
It is small, convenient for the development of experiment.
3. operating process of the present invention is easy, prior art tedious process and a large amount of manpower, material resources and financial resources of consuming.
4. the feeding device that the present invention uses effectively in experimental subjects limitation in a smaller space, is deposited experimental subjects
Living and oviposition situation can have to be got information about very much.
5. the feeding device that the present invention uses may be reused, and equipment used is through more economical and more practical than the prior art.
Specific implementation mode
The equipment and reagent used in experiment include：Be uniformly distributed 40 apertures poly (methyl methacrylate) plate (10cm × 25cm ×
8mm), hole diameter 8mm, depth 5mm；The man-made feeds that certain proportion is prepared；Water-glycerol solution；Coverslip；Artificial gas
Wait case；Anatomical lens.
The present invention is operated according to following case：
Embodiment 1
The implementation of ellipse dispersion life table experiment, the man-made feeds configured are applied in the aperture of poly (methyl methacrylate) plate
Portion, naturally dry.
Single ellipse dispersion female individuals are put into aperture, it is close to cover the bigger coverslip progress of diameter than hole diameter
Envelope, smears 1 around coverslip:1 water-glycerol solution, to ensure that humidity keeps within limits, having in aperture
An ellipse dispersion female individuals are respectively put into all 40 apertures of machine glass plate, poly (methyl methacrylate) plate is placed on temperature 32
DEG C, humidity 70%, growth cabinet in, timing daily is observed primary, and the daily egg laying amount of accurate recording ellipse dispersion obtains
The data arrived are used for subsequent statistical analysis.
Embodiment 2
The implementation of tyrophagus putrescentiae life table experiment, the man-made feeds configured are applied in the aperture of poly (methyl methacrylate) plate
Portion, naturally dry.
Single tyrophagus putrescentiae female individuals are put into aperture, it is close to cover the bigger coverslip progress of diameter than hole diameter
Envelope, smears 1 around coverslip:1 water-glycerol solution, to ensure that humidity keeps within limits, having in aperture
A tyrophagus putrescentiae female individuals are respectively put into all 40 apertures of machine glass plate, poly (methyl methacrylate) plate is placed on temperature 32
DEG C, humidity 70%, growth cabinet in, timing daily is observed primary, and the daily egg laying amount of accurate recording tyrophagus putrescentiae obtains
Data be used for subsequent statistical analysis.
Claims (2)
1. a kind of small arthropod life table experimental method, which is characterized in that steps are as follows:(1) divides poly (methyl methacrylate) plate
At suitable size, and conical apertures are uniformly stamped above；
(2) smears prior proportioned feed inside the aperture accomplished fluently, and is used as the food of experimental subjects；
(3) single experimental subjects is put into aperture by, covered, and water-glycerol solution is smeared in the surrounding of coverslip；
(4) poly (methyl methacrylate) plate is put into growth cabinet by；
(5) periodically observes and records experimental data daily;
The small arthropod is mite class.
2. small arthropod life table experimental method according to claim 1, which is characterized in that step（1）It is used
Poly (methyl methacrylate) plate aperture be diameter 8mm depth 5mm conical apertures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610204968.5A CN105766809B (en) | 2016-04-05 | 2016-04-05 | A kind of small arthropod life table experimental method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610204968.5A CN105766809B (en) | 2016-04-05 | 2016-04-05 | A kind of small arthropod life table experimental method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105766809A CN105766809A (en) | 2016-07-20 |
| CN105766809B true CN105766809B (en) | 2018-10-19 |
Family
ID=56395616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610204968.5A Expired - Fee Related CN105766809B (en) | 2016-04-05 | 2016-04-05 | A kind of small arthropod life table experimental method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105766809B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107822670B (en) * | 2017-09-22 | 2020-03-10 | 湖北大学 | Rapid and accurate spider intestinal tract dissection method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642079B (en) * | 2009-08-27 | 2012-05-30 | 广东省农业科学院作物研究所 | Classified raising method and imago raising device of corn borers |
| KR200456554Y1 (en) * | 2009-12-30 | 2011-11-07 | 주식회사 오상킨섹트 | Cell tray |
| CN202873580U (en) * | 2012-09-18 | 2013-04-17 | 江西省新龙生物科技有限公司 | Portable insect breeding plate |
| CN203262061U (en) * | 2013-05-20 | 2013-11-06 | 中国农业科学院植物保护研究所 | Small raising room for experiments related to mites |
| CN203723281U (en) * | 2014-01-08 | 2014-07-23 | 广东省烟草南雄科学研究所 | Harpactor fuscipes nymph large-scale breeding vessel |
| CN204994448U (en) * | 2015-09-10 | 2016-01-27 | 华中农业大学 | Box is raised to bollworm |
-
2016
- 2016-04-05 CN CN201610204968.5A patent/CN105766809B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN105766809A (en) | 2016-07-20 |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181019 |