CN202873585U - Tetranychid mite biological assay device - Google Patents
Tetranychid mite biological assay device Download PDFInfo
- Publication number
- CN202873585U CN202873585U CN 201220523322 CN201220523322U CN202873585U CN 202873585 U CN202873585 U CN 202873585U CN 201220523322 CN201220523322 CN 201220523322 CN 201220523322 U CN201220523322 U CN 201220523322U CN 202873585 U CN202873585 U CN 202873585U
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- mite
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Abstract
The utility model relates to a tetranychid mite biological assay device which comprises a culture dish, double-layer filter paper is laid inside the culture dish, a plurality of dark blue glass slides are placed on the double-layer filter paper, one ends of the dark blue glass slides are spliced with rectangular double-coating tapes, a plurality of rectangular grids are arranged on the rectangular double-coating tapes, a fresh-keeping film is covered on the top of the culture dish, and a plurality of air holes are formed in the fresh-keeping film. The tetranychid mite biological assay device can be reused for many times, and is easy to popularize in the further and low in cost. Tests for mite eggs provided for the tests can be conducted in an established simulated natural ecological environment, so that the mite eggs provided for the tests can truly reflect real effects of methods for the tests. Observations become easy, and the tetranychid mite biological assay device is especially suitable for fast biological assaying tests. The problem that the mite eggs provided for the tests die unnaturally is solved, and results of the biological assays are more real. The tetranychid mite biological assay device is easy to popularize and apply, and suitable for the biological assaying tests for various mite eggs and various fast biological assaying tests. The operation is convenient, and work efficiency is increased by more than 30 times compared with a conventional method.
Description
Technical field
The utility model relates to a kind of tetranychid biometric apparatus.
Background technology
Do agricultural fruit and vegetable field mite class ovum control and observe and predict and give birth in the survey work, often can do development progress, resistance and give birth to the observation of the aspects such as surveys mite class ovum, can use various foster mite utensils and life survey method.Support the mite utensil and give birth to the survey method, supporting the mite utensil has the commercially available product of producing by batch, finalize the design, and also has various new examinations (formula) product to continue to bring out.Test in the work and give birth to test in the indoor mite class of reality at present, employing is given birth to test to the mite class in agricultural fruit and vegetable field and is tested, what majority adopted is " slide method ", and we find that in actual indoor biometrics experiment work " slide method " tests the result to living test is the larger method of a kind of error.And give birth to test and test in the work and be widely used multiple.Along with the progressively popularization of green agriculture, organic farming theory, in the control of doing agricultural fruit and vegetable field mite class with observe and predict and give birth in the survey work, how to adopt method efficient, environmental protection to come the work of kill pests, more and more be subject to people's attention.For this reason, at first wanting ready-made be to give birth to test to test efficient in the work.
The utility model content
For solving the problems of the technologies described above, the utility model provides a kind of tetranychid biometric apparatus simple in structure, easy to use.
Tetranychid biometric apparatus of the present utility model, comprise culture dish, described culture dish inner mast is established double-deck filter paper, be placed with some navy blue slides on the double-deck filter paper, the bonding rectangle Double-face gummed paper of one end at navy blue slide top, a plurality of rectangle grids are set on the rectangle Double-face gummed paper, and the top cover of culture dish is established preservative film, and a plurality of air-vents are set on the preservative film.
Tetranychid biometric apparatus of the present utility model, described culture dish are white circular porcelain dish.
Tetranychid biometric apparatus of the present utility model, described navy blue slide is the navy blue art paper.
Tetranychid biometric apparatus of the present utility model, the diameter of described air-vent are 0.01-0.02cm.
Compared with prior art the beneficial effects of the utility model are:
1) can be repeatedly used, be easy in the future promote, with low cost;
2) can be based upon under the ecotope of imitative nature and supply the test of examination acarid ovum, make for examination acarid ovum with this to reflect really the true effect that supplies method for testing;
3) be convenient to observe, the test that suitable especially quick bio is measured is used;
4) solved for the unusual death problem of trying the acarid ovum, made the result who gives birth to survey have more authenticity;
5) easy to utilize, be applicable to the need of multiple acarid ovum, multiple quick bio determination test;
6) easy to operate, than conventional method, improve work efficiency more than 30 times.
Description of drawings
Fig. 1 is the structural representation of the described a kind of tetranychid biometric apparatus of the utility model embodiment;
Fig. 2 is the structural representation of preservative film in described kind of tetranychid biometric apparatus of the utility model embodiment.
Among the figure:
1, culture dish; 2, navy blue slide; 3, rectangle Double-face gummed paper; 4, preservative film; 5, air-vent.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples are used for explanation the utility model, but are not used for limiting scope of the present utility model.
Shown in Fig. 1-2, a kind of tetranychid biometric apparatus, comprise culture dish 1, described culture dish 1 inner mast is established double-deck filter paper, be placed with some navy blue slides 2 on the double-deck filter paper, the bonding rectangle Double-face gummed paper 3 of an end at navy blue slide 2 tops arranges a plurality of rectangle grids on the rectangle Double-face gummed paper 3, the top cover of culture dish 1 is established preservative film 4, and a plurality of air-vents 5 are set on the preservative film 4.
Tetranychid biometric apparatus of the present utility model, described culture dish 1 is white circular porcelain dish.
Tetranychid biometric apparatus of the present utility model, described navy blue slide 2 is the navy blue art paper.
Tetranychid biometric apparatus of the present utility model, the diameter of described air-vent 5 are 0.01-0.02cm.
Tetranychid biometric apparatus of the present utility model may further comprise the steps when concrete the use:
1, prepare the consistent ovum of indoor feeding physiological status: front 3-4 days of test, connect a large amount of tetranychids with fresh without the worm bean seedlings, obtain the consistent ovum grain of developmental condition, the milky during for primiparity.
2, the navy blue art paper is simultaneously printed 1.0cm * 0.5cm rectangle form, then art paper is cut into the slide size, Double-face gummed paper is cut into the wide 2cm*2cm of being of long *, be affixed on an end of art paper, then at microscopically with the soft wet hair nib milky ovum grain that adheres to of picking bean seedlings blade silk screen gently, do not touch the ovum grain as far as possible, only depend on silk screen its transfer to be adhered on the Double-face gummed paper of art paper, sticking 10 ovum in each grid, every sticking 3 little lattice amount to 30 ovum.
3, medicament preparation, former medicinal organic solvent (acetone, dimethyl sulfoxide (DMSO), methyl alcohol etc.) is configured to mother liquor, uses the Tween 80 of 0.05%～0.1%Triton X-100(or 0.1% again) aqueous solution is according to 5～7 series mass concentration of the method for geometric ratio or equal difference configuration.
4, chemicals treatment will be carried and be taken out after the ovum art paper is dipped in the 5s that vibrates gently in the liquid, suck unnecessary liquid with blotting paper, place the ceramic whiteware dish that is lined with filter paper, and good plastics insurance film covers to use light transmission.Every processing repeats for 4 times, and establishes the processing that does not contain medicament (containing all organic solvents and emulsifier) and make blank.
5, raise and observe, process the container that tries worm to place temperature be that 25 ± 1 ℃, photoperiod are to raise under the L:D=16:8 h condition filling.
6,Check that 72h institutes an inquiry the hatching situation of incubating grain after processing, look into once every 24h that until 240h behind the medicine, the each inspection rejected the ovum grain of having hatched, record hatching number, unhatched ovum is decided to be dead borer population.
The method has solved the indoor biometrics problem of spider mite kind ovum, and blue art paper is easy to the later stage observed and recorded.
The above only is preferred embodiment of the present utility model; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the utility model know-why; can also make some improvement and modification, these improve and modification also should be considered as protection domain of the present utility model.
Claims (4)
1. tetranychid biometric apparatus, comprise culture dish (1), it is characterized in that: described culture dish (1) inner mast is established double-deck filter paper, be placed with some navy blue slides (2) on the double-deck filter paper, the bonding rectangle Double-face gummed paper of one end (3) at navy blue slide (2) top, a plurality of rectangle grids are set on the rectangle Double-face gummed paper (3), and the top cover of culture dish (1) is established preservative film (4), and a plurality of air-vents (5) are set on the preservative film (4).
2. tetranychid biometric apparatus according to claim 1, it is characterized in that: described culture dish (1) is white circular porcelain dish.
3. tetranychid biometric apparatus according to claim 1, it is characterized in that: described navy blue slide (2) is the navy blue art paper.
4. tetranychid biometric apparatus according to claim 1, it is characterized in that: the diameter of described air-vent (5) is 0.01-0.02cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220523322 CN202873585U (en) | 2012-10-12 | 2012-10-12 | Tetranychid mite biological assay device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220523322 CN202873585U (en) | 2012-10-12 | 2012-10-12 | Tetranychid mite biological assay device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202873585U true CN202873585U (en) | 2013-04-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220523322 Expired - Fee Related CN202873585U (en) | 2012-10-12 | 2012-10-12 | Tetranychid mite biological assay device |
Country Status (1)
| Country | Link |
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| CN (1) | CN202873585U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931419A (en) * | 2021-03-09 | 2021-06-11 | 扬州大学 | Method for in vitro breeding of soybean hornworm by using soybean leaves |
| CN114617105A (en) * | 2022-03-24 | 2022-06-14 | 广西特色作物研究院 | Mite egg bioassay method |
-
2012
- 2012-10-12 CN CN 201220523322 patent/CN202873585U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931419A (en) * | 2021-03-09 | 2021-06-11 | 扬州大学 | Method for in vitro breeding of soybean hornworm by using soybean leaves |
| CN114617105A (en) * | 2022-03-24 | 2022-06-14 | 广西特色作物研究院 | Mite egg bioassay method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130417 Termination date: 20131012 |