CN102621278A - Method for quickly detecting sensibility of two-spotted spider mite to avermectin - Google Patents
Method for quickly detecting sensibility of two-spotted spider mite to avermectin Download PDFInfo
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- CN102621278A CN102621278A CN201210066020XA CN201210066020A CN102621278A CN 102621278 A CN102621278 A CN 102621278A CN 201210066020X A CN201210066020X A CN 201210066020XA CN 201210066020 A CN201210066020 A CN 201210066020A CN 102621278 A CN102621278 A CN 102621278A
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Abstract
The invention relates to a method for quickly detecting the sensibility of a two-spotted spider mite to avermectin. A sealed and stable space is formed by mainly adopting a culture dish film sealing method; the concentration of a drug is enabled to be appropriate, uniform and stable; the drug effect of an agricultural pesticide is enabled to appear adequately within a shorter time; and the sensibility of the two-spotted spider mite to the avermectin can be quickly and accurately detected.
Description
Technical field
The present invention relates to the method for a kind of fast detecting Tetranychus urticae to AVM susceptibility.
Background technology
Tetranychus urticae is a kind of important worldwide harmful mite, and this mite is prone to develop immunity to drugs to chemical pesticide, and the Tetranychus urticae on the Australian cotton has just produced the resistance to the action of a drug after Bi Fenning registers 4 years.The eighties in 20th century, Tetranychus urticae imported China into, because it imports China into and just many kinds of agricultural chemicals has been produced resistance in the past, mainly relied on chemical pesticide in the domestic control, and this makes that the development of drug resistance of Tetranychus urticae is rapid, and causing harm is on the rise.
AVM is as the antibiotics insecticidal/acaricidal agent; The mechanism of action is to stimulate polypide to produce GABA; The transmission of blocking-up kinesitherapy nerve information makes insect paralysis, food refusal, slow-action or motionless rapidly in several hrs, and its mechanism of action is unique; See that light be prone to decompose, the inanimate object accumulation and residual, be difficult for contaminated environment, become the first-selected medicament that people prevent and treat Tetranychus urticae.But laboratory experiment shows uses 20 generations of the continuous seed selection of AVM, and Tetranychus urticae can produce higher resistance to AVM.Because the medicining condition of China each department exists than big-difference, so Tetranychus urticae is also different to the resistance of AVM, in order to instruct peasant's rational use of medicines better, just need detect the susceptibility of Tetranychus urticae to AVM.
At present; Resistance monitoring less to polypide, the Tetranychus urticae that is prone to knot adopts slide infusion process or centrifuge tube method more; The slide infusion process just can be obtained a result after need handling 24h; Be difficult to reach the purpose of fast detecting, and the slide infusion process is the space that Tetranychus urticae provides an opening, useful space pesticide concentration is low excessively; The centrifuge tube method is owing to manage end excessive concentration, and mouth of pipe concentration is low excessively, and it is inaccurate to cause mortality ratio to be measured, and therefore, the urgent need searching is a kind of can quick and precisely to detect the method for Tetranychus urticae to AVM susceptibility.
Summary of the invention
To the problems referred to above, the present invention proposes a kind of method that quick and precisely detects Tetranychus urticae to AVM susceptibility, adopt this method, save time, laborsaving, quick, easy.
The present invention mainly adopts double dish envelope embrane method, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, the reservation healthy individuals;
(3) AVM is diluted to 0.1-0.4mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, seal, form airtight medicine film space, and check mortality ratio after placing growth cabinet 1h-4h, and calculate mortality ratio with sealing film.
Because mite class individuality is little, the creep speed piece is escaped easily; And weave silk easily and knot, the bull polypide flocks together, and causes the later stage difficult microscopic; Be difficult to whether accurately judge polypide death, can't obtain accurate result, so the present invention adopts double sticky tape to fix female one-tenth mite.Generally double sticky tape is cut into 2-3cm length; Be attached to an end of microslide; Throw off the scraps of paper on the viscose glue with tweezers; The female one-tenth mite consistent with No. zero writing brush picking size, that body colour is bright-coloured, action is active, with its back be bonded on the double sticky tape but do not cling mite foot, mite must and mouthpart, unite thereby avoided Tetranychus urticae to knot effectively.The about 7-8cm of microslide length, double faced adhesive tape length 2-3cm, the residue length of microslide can be convenient to operation when soaking medicine and microscopy.
In this process, if female one-tenth mite quantity is very little, can cause experimental error too big, make the reliability of experimental result reduce; Too much, can make troublesome poeration, workload strengthens; Therefore, in order to guarantee the reliability of experimental result, fixing female one-tenth mite 30-50 head on the every microslide; Can be divided into multirow; After the later stage weeds out sluggish insect out of order, still can guarantee to give birth to test like this and test reliability and accuracy, can not increase too many workload again, also be convenient to observe.
The double dish diameter specifications that adopts in the experimentation is 16cm, for guaranteeing the reliability of experimental result, three band mite microslides that flooded same concentration abamectin solution of general placement in the same double dish.
Above-mentioned band mite microslide is placed growth cabinet, and in order to adapt to the temperature and humidity of Tetranychus urticae and Tetranychus urticae host plant growth, the temperature of growth cabinet is 25 ± 1 ℃, and relative humidity is 75 ± 5%.Behind the 1h, use binocular vision, reject dead and the torpescence individuality, keep healthy individuals, preferably keep the 20-40 head, both guaranteed the reliability of experimental result, can not increase workload again.
Above-mentioned healthy individuals is immersed in the abamectin solution of dilution, shake gently and take out and blot rapidly unnecessary soup around the mite body behind the 5s.In order to guarantee to detect the result in the short period, the present invention is 0.1-0.4mg/L with the abamectin solution concentration dilution.
Band mite microslide after the immersion liquid is put into double dish, seal, form an airtight medicine film space, thereby the concentration that has guaranteed in effective space, to keep agricultural chemicals is suitable, evenly and stable, improved the reliability of experimental result with sealing film; In order to make pesticide efficacy have the sufficient time to be able to manifest, the present invention is decided to be 1-4h with the drug action time, through verification experimental verification, and preferred 3h.
In addition owing to be that the present invention measured in the short time, can't be only with complete death as criterion.The present invention has adopted successively and has not shaken slide, vibrations slide, flips examination worm, vibrations slide+flip with the writing brush comparison test of examination worm four kinds of microscopy methods with writing brush; And observation is tried several legs actions of worm compare for whether dead standard; Final definite; Shake slide during microscopy earlier, place then under the anatomical lens, movable examination worm is worm alive; Examination worm for motionless flips with writing brush again, bump the back movable active still be the worm that lives, and just reaction equation make a movement be dead worm.
For reliability and the accuracy that guarantees experimental result; The each experiment of the present invention all is provided with control group, promptly replaces abamectin solution with clear water, and other parameter is all identical with the present invention; The mortality ratio of control group meets the requirement of biologicall test test standard below 5%.All experimental results of the present invention all are that benchmark is proofreaied and correct with the control group, and mortality ratio is corrected mortality.
In sum; The present invention adopts double dish envelope embrane method to form an airtight stable environment; In the useful space, make pesticide concentration suitable, even and stable; Improved the reliability of experimental result and made pesticide efficacy be able to fully manifest within a short period of time, not only reached easy to operate requirement but also improved the speed of action of medicament.
Embodiment
Embodiment 1
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 30;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 20 of reservation healthy individuals;
(3) AVM is diluted to 0.1mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 1h, and to calculate corrected mortality with reference to control test be 10.7% with sealing film.
Embodiment 2
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 40;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 30 of reservation healthy individuals;
(3) AVM is diluted to 0.2mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 1h, and to calculate corrected mortality with reference to control test be 13.5% with sealing film.
Embodiment 3
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 50;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 40 of reservation healthy individuals;
(3) AVM is diluted to 0.4mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 1h, and to calculate corrected mortality with reference to control test be 20.2% with sealing film.
Embodiment 4
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 30;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 20 of reservation healthy individuals;
(3) AVM is diluted to 0.1mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 2h, and to calculate corrected mortality with reference to control test be 19.4% with sealing film.
Embodiment 5
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 40;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 30 of reservation healthy individuals;
(3) AVM is diluted to 0.2mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 2h, and to calculate corrected mortality with reference to control test be 25.0% with sealing film.
Embodiment 6
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 50;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 40 of reservation healthy individuals;
(3) AVM is diluted to 0.4mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 2h, and to calculate corrected mortality with reference to control test be 47.8% with sealing film.
Embodiment 7
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 30;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 20 of reservation healthy individuals;
(3) AVM is diluted to 0.1mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 3h, and to calculate corrected mortality with reference to control test be 19.4% with sealing film.
Embodiment 8
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 40;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 30 of reservation healthy individuals;
(3) AVM is diluted to 0.2mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 3h, and to calculate corrected mortality with reference to control test be 47.1% with sealing film.
Embodiment 9
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 50;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 40 of reservation healthy individuals;
(3) AVM is diluted to 0.4mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 3h, and to calculate corrected mortality with reference to control test be 67.7% with sealing film.
Embodiment 10
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 30;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 20 of reservation healthy individuals;
(3) AVM is diluted to 0.1mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 4h, and to calculate corrected mortality with reference to control test be 20.0% with sealing film.
Embodiment 11
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 40;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 30 of reservation healthy individuals;
(3) AVM is diluted to 0.2mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 4h, and to calculate corrected mortality with reference to control test be 50.0% with sealing film.
Embodiment 12
A kind of fast detecting Tetranychus urticae is to the method for AVM susceptibility, and its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end, every sticking 50;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, 40 of reservation healthy individuals;
(3) AVM is diluted to 0.4mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body with thieving paper rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, and seal, and form airtight medicine film space, and check mortality ratio after placing growth cabinet 4h, and to calculate corrected mortality with reference to control test be 96.7% with sealing film.
With the experimental result among the embodiment 1-12 gather with table 1 in:
The Tetranychus urticae of table 1 embodiment 1-12 fast measuring is to the susceptibility of AVM
Find that by table 1 the method is moderate to the speed of action of Tetranychus urticae, all formed gradient preferably between each time period behind the medicine in the 4h and each concentration of same observing time.
Comparative example 1
Adopt this area centrifuge tube method commonly used, measure the susceptibility of Tetranychus urticae to AVM, drug concentration is identical with action time and the present invention, and the result sees table 2.
Table 2 centrifuge tube method fast measuring Tetranychus urticae is to the susceptibility of AVM
Find out that from table 2 the method is very fast to the speed of action of Tetranychus urticae, each the time period difference behind the medicine in the 4h is little; Concentration is high more, and the time is more little to the influence of mortality ratio, all can not show good gradient between each time period and each concentration; The poor reliability of experimental result, and during check result, the polypide majority is knotted and is united; The separately microscopy again that needs a head, troublesome poeration.
Comparative example 2
Adopt this area slide infusion process commonly used, measure the susceptibility of Tetranychus urticae to AVM, drug concentration is identical with action time and the present invention, and the result sees table 3.
Can be found out that by table 3 the dead speed of Tetranychus urticae is very slow behind the medicine, the method is difficult to knock down Tetranychus urticae at short notice.
In sum, though the centrifuge tube method is fast to the Tetranychus urticae speed of action, because drug concentration heterogeneity in the whole space all can not show good gradient between each time period and each concentration, the poor reliability of experimental result; Though slide infusion process experimental result is reliable relatively, the time that needs is oversize, can't realize fast detecting; The present invention is moderate to the speed of action of Tetranychus urticae, has all formed gradient preferably between each time period behind the medicine in the 4h and each concentration of same observing time, and action time is short, detects fast, and the result accurately and reliably.
Claims (5)
1. a fast detecting Tetranychus urticae is to the method for AVM susceptibility, and it is characterized in that: its concrete steps are:
(1) the picking size is consistent, body colour is bright-coloured, the active female one-tenth mite that takes action, and it neatly is fixed in microslide one end;
(2) above-mentioned band mite microslide is placed growth cabinet 1h after, use binocular vision, reject dead individual with torpescence, the reservation healthy individuals;
(3) AVM is diluted to 0.1-0.4mg/L, immerses band mite microslide in the soup, shake gently, take out behind the 5S, blot the mite body rapidly and reach unnecessary soup on every side;
(4) will be with the mite microslide to put into double dish, seal, form airtight medicine film space, check mortality ratio after placing growth cabinet 1h-4h with sealing film.
2. a kind of fast detecting Tetranychus urticae according to claim 1 is characterized in that the method for AVM susceptibility: in the step (1) on every microslide female one-tenth mite be the 30-50 head.
3. a kind of fast detecting Tetranychus urticae according to claim 1 is characterized in that the method for AVM susceptibility: the temperature of growth cabinet is 25 ± 1 ℃, and relative humidity is 75 ± 5%.
4. a kind of fast detecting Tetranychus urticae according to claim 1 is characterized in that the method for AVM susceptibility: keeping healthy female one-tenth mite on every microslide in the step (2) is the 20-40 head.
5. a kind of fast detecting Tetranychus urticae according to claim 1 is characterized in that the method for AVM susceptibility: the preferred 3h of time in the step (4).
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451290A (en) * | 2013-08-30 | 2013-12-18 | 中国农业科学院蔬菜花卉研究所 | Primer pair, kit and detection method for detecting abamectin resistance of tetranychus urticae |
| CN103675255A (en) * | 2013-12-16 | 2014-03-26 | 中国农业大学 | Indoor biological assay method of aphides |
| CN112098638A (en) * | 2020-07-30 | 2020-12-18 | 山东省农业科学院植物保护研究所 | Method for determining toxicity of pesticide to osmia larva |
| CN113218820A (en) * | 2021-04-28 | 2021-08-06 | 福建新农大正生物工程有限公司 | Evaluation method for body surface wettability of spider mites |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080160556A1 (en) * | 2006-12-13 | 2008-07-03 | Industrial Technology Research Institute | Bioassay element and producing method thereof |
| CN101571532A (en) * | 2008-04-29 | 2009-11-04 | 中国农业科学院植物保护研究所 | Novel method for measuring biological activity of biological pesticides |
| CN101788545A (en) * | 2010-02-04 | 2010-07-28 | 国际竹藤网络中心 | Pesticide biological activity determination method |
-
2012
- 2012-03-14 CN CN201210066020.XA patent/CN102621278B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080160556A1 (en) * | 2006-12-13 | 2008-07-03 | Industrial Technology Research Institute | Bioassay element and producing method thereof |
| CN101571532A (en) * | 2008-04-29 | 2009-11-04 | 中国农业科学院植物保护研究所 | Novel method for measuring biological activity of biological pesticides |
| CN101788545A (en) * | 2010-02-04 | 2010-07-28 | 国际竹藤网络中心 | Pesticide biological activity determination method |
Non-Patent Citations (4)
| Title |
|---|
| 丁晓帆 等: "甲胺基阿维菌素苯甲酸盐对南方根结线虫的毒力、防效及安全性研究", 《南京农业大学学报》 * |
| 张志刚 等: "二斑叶螨对螺螨酯抗药性及对18种杀螨剂交互抗性", 《植物保护》 * |
| 裴晖等: "新化合物HNPC-A 3066的杀螨活性及田间防治效果研究", 《农药学学报》 * |
| 黄素青等: "农田害螨的几种生物测定方法", 《植物保护》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451290A (en) * | 2013-08-30 | 2013-12-18 | 中国农业科学院蔬菜花卉研究所 | Primer pair, kit and detection method for detecting abamectin resistance of tetranychus urticae |
| CN103675255A (en) * | 2013-12-16 | 2014-03-26 | 中国农业大学 | Indoor biological assay method of aphides |
| CN112098638A (en) * | 2020-07-30 | 2020-12-18 | 山东省农业科学院植物保护研究所 | Method for determining toxicity of pesticide to osmia larva |
| CN113218820A (en) * | 2021-04-28 | 2021-08-06 | 福建新农大正生物工程有限公司 | Evaluation method for body surface wettability of spider mites |
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| CN102621278B (en) | 2014-12-17 |
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