CN113749056A - Culex spawning and hatching method - Google Patents
Culex spawning and hatching method Download PDFInfo
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- CN113749056A CN113749056A CN202111014010.7A CN202111014010A CN113749056A CN 113749056 A CN113749056 A CN 113749056A CN 202111014010 A CN202111014010 A CN 202111014010A CN 113749056 A CN113749056 A CN 113749056A
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- spawning
- culex
- cup
- hatching
- female
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- 241000256054 Culex <genus> Species 0.000 title claims abstract description 58
- 230000012447 hatching Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 22
- 241000255925 Diptera Species 0.000 claims abstract description 37
- 239000008280 blood Substances 0.000 claims abstract description 23
- 210000004369 blood Anatomy 0.000 claims abstract description 23
- 235000013601 eggs Nutrition 0.000 claims abstract description 16
- 238000011534 incubation Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 230000006408 female gonad development Effects 0.000 claims abstract description 4
- 230000017448 oviposition Effects 0.000 claims description 22
- 241001494479 Pecora Species 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 241000238631 Hexapoda Species 0.000 description 7
- 230000009286 beneficial effect Effects 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 241000256135 Chironomus thummi Species 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000624 ovulatory effect Effects 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 208000001490 Dengue Diseases 0.000 description 1
- 206010012310 Dengue fever Diseases 0.000 description 1
- 206010014596 Encephalitis Japanese B Diseases 0.000 description 1
- 201000006353 Filariasis Diseases 0.000 description 1
- 201000005807 Japanese encephalitis Diseases 0.000 description 1
- 241000710842 Japanese encephalitis virus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 208000003152 Yellow Fever Diseases 0.000 description 1
- 208000020329 Zika virus infectious disease Diseases 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 208000025729 dengue disease Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a method for laying eggs and hatching culex mosquitoes, which comprises the following steps: early preparation: feeding blood to the emerged female culex mosquitoes in a mosquito cage, and waiting for the ovary development of the female culex mosquitoes; spawning for the first time: placing the spawning cup into a mosquito cage, enabling culex female mosquitoes to spawn in the spawning cup, and taking out the spawning cup after 24 hours; the inner wall of the spawning cup is tightly attached to a filter screen, and the water surface in the spawning cup exceeds the bottom of the filter screen; hatching: hatching ova produced by female culex mosquitoes in a spawning cup; after 24 hours, removing the filter screen, and putting the unhatched eggs left on the filter screen into another spawning cup for further incubation for 24 hours; and (3) spawning and hatching for the second time: feeding blood to female culex in the mosquito cage after the female culex spawns for the first time, putting the spawning cup into the mosquito cage, and taking out the spawning cup after 24 hours; repeating the incubating step. By using the method, the hatched larvae are counted more conveniently and more accurately; provides necessary conditions for controlling the larva breeding density in the next step.
Description
Technical Field
The invention relates to the technical field of culex artificial feeding, in particular to a culex oviposition and incubation method.
Background
The male sterile insect release technology is used as a new technology in pest control, and by cultivating the male sterile insects of pests and releasing the male sterile insects to a pest breeding place, the male sterile insects can not propagate offspring after mating with wild female insects, so that the insects can be reduced and even eliminated. Compared with the traditional pesticide disinsection strategy, the technology is environment-friendly and has no influence on other insects and the environment. Mosquitoes are of great interest as intermediate hosts to other pathogens such as dengue fever, Zika, malaria, yellow fever, filariasis, Japanese encephalitis, and the like, as "mosquito vector control" measures to control the amount of transmitted virus to reduce human contact.
In the existing breeding method, when culex mosquitos lay eggs in water, the female mosquitoes laying eggs stop on the water surface to lay eggs, and the culex ovums begin to hatch after more than 20 hours of egg laying; along with the hatching of the ooval, a plurality of adult mosquito corpses can appear on the water surface, and the larvae, the ooval and the adult mosquito corpses are mixed together, so that the larva feeding in the next step is extremely unfavorable, the density control is inaccurate, and the larva synchronism is poor; is not beneficial to the standardized operation and cost control of mass production. In a large-scale culex male mosquito release plan, the spawning and hatching of female mosquitoes is an important production link, so that a simple, efficient and stable spawning and hatching method needs to be invented.
Disclosure of Invention
The invention aims to provide a method for the oviposition and hatching of culex mosquitoes, which is beneficial to large-scale standardized feeding and further creates good conditions for feeding culex larvae.
In order to realize the purpose, the technical scheme is as follows:
a method for the oviposition and incubation of culex includes the following steps:
early preparation: feeding blood to the emerged female culex mosquitoes in a mosquito cage, and waiting for the ovary development of the female culex mosquitoes;
spawning for the first time: placing the spawning cup into a mosquito cage, enabling culex female mosquitoes to spawn in the spawning cup, and taking out the spawning cup after 24 hours; the inner wall of the spawning cup is tightly attached to a filter screen, and the water surface in the spawning cup exceeds the bottom of the filter screen;
hatching: hatching the egg valve produced by female culex mosquitoes in the egg laying cup; after 24 hours, removing the filter screen, and putting the unhatched eggs left on the filter screen into another egg laying cup for further incubation for 24 hours;
and (3) spawning and hatching for the second time: feeding blood to female culex in the mosquito cage after the female culex spawns for the first time, putting the spawning cup into the mosquito cage, and taking out the spawning cup after 24 hours; repeating the incubating step.
Further, in the preliminary preparation step, the female culex was fed with blood for 3 days.
Further, the blood is sheep blood.
Further, the mesh size of the filter screen is 18-30 meshes.
Further, the filter mesh screen is made of a stainless steel mesh.
Further, the culex mosquitoes are killed after completing the second oviposition.
Further, the step requires that the environmental conditions are 26 + -1 deg.C temperature and 75 + -10% humidity.
Compared with the prior art, the invention has the beneficial effects that:
by using the method for oviposition and incubation of the culex midge, the oviposition cup is replaced periodically, so that ova and incubated larvae can be separated, and the incubated larvae can be counted more conveniently and more accurately; necessary conditions are provided for controlling the larva breeding density in the next step; because the culex ovulatory valve can not be preserved, the female culex can be hatched quickly after spawning; the method is helpful for improving the growth synchronism of larvae. The method is simple to operate, low in cost and beneficial to large-scale production and use.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic flow chart of a method for oviposition and incubation of Culex.
Detailed Description
The embodiments of the present disclosure are described in detail below with reference to the accompanying drawings.
The embodiments of the present disclosure are described below with specific examples, and other advantages and effects of the present disclosure will be readily apparent to those skilled in the art from the disclosure in the specification. It is to be understood that the described embodiments are merely illustrative of some, and not restrictive, of the embodiments of the disclosure. The disclosure may be embodied or carried out in various other specific embodiments, and various modifications and changes may be made in the details within the description without departing from the spirit of the disclosure. It is to be noted that the features in the following embodiments and examples may be combined with each other without conflict. All other embodiments, which can be derived by a person skilled in the art from the embodiments disclosed herein without making any creative effort, shall fall within the protection scope of the present disclosure.
A method for laying eggs and hatching culex comprises the following steps:
first, early preparation: feeding blood to the emerged female culex mosquitoes in a mosquito cage, and waiting for the ovary development of the female culex mosquitoes.
Specifically, a batch of culex pupae with the same growth cycle is arranged in the mosquito cage, and after five days of feathering of the culex pupae, sheep blood is continuously fed for three days. Female mosquitoes develop gradually when drinking blood, and during the period, the female mosquitoes mate with male mosquitoes.
Wherein, the blood is sheep blood which is less than infectious diseases of other pig blood or cattle blood and is relatively safe. Sheep blood was examined and quarantined and supplied by a specialized biologics company.
Step two, spawning for the first time: placing the spawning cup into a mosquito cage, enabling culex female mosquitoes to spawn in the spawning cup, and taking out the spawning cup after 24 hours; the inner wall of the spawning cup is tightly attached to the filter screen, and the water surface in the spawning cup exceeds the bottom of the filter screen, so that spawning female mosquitoes can directly contact the water surface.
Specifically, female mosquitoes can lay eggs once in 24 hours, and the spawning cup is taken out to check whether a culex corpse is on a filter screen or not and is cleared in time.
Wherein, the filter screen is made of 18-30 mesh stainless steel mesh, the hatched larvae can be left in water through the mesh screen, and the hatched egg shells and adult mosquito carcasses can be left on the filter screen, so that the treatment is convenient.
Thirdly, hatching: hatching ova produced by female culex mosquitoes in a spawning cup; after 24 hours, the filter screen was removed, and the unhatched eggs left on the filter screen were felled in another egg laying cup and incubated for another 24 hours.
Specifically, the same batch of eggs need to be hatched twice, and each hatching time is 24 hours. The purpose of replacing the spawning cup is to ensure that the larvae incubated in the same spawning cup have good synchronism, namely, the larvae are ensured to be consistent in size. If the eggs are cut in the same egg laying cup for two days for hatching, the larvae have different sizes, so that the subsequent larva breeding difficulty is high.
Fourthly, spawning and hatching for the second time: feeding blood to female culex in the mosquito cage after the female culex spawns for the first time, putting the spawning cup into the mosquito cage, and taking out the spawning cup after 24 hours; the incubation step is repeated.
Specifically, female mosquitoes are fed with blood and lay eggs once. The female mosquitoes are fed with sheep blood for two days after the first oviposition interval of 4 days, and then oviposit for the second time.
And fifthly, killing the culex mosquitoes finishing the second oviposition.
Specifically, the female mosquitoes are produced twice before, the number of eggs is the largest, and the larva quality is the best.
In this example, all steps require ambient conditions of 26. + -. 1 ℃ temperature and 75. + -. 10% humidity.
By using the method for oviposition and incubation of the culex midge, the oviposition cup is replaced periodically, so that ova and incubated larvae can be separated, and the incubated larvae can be counted more conveniently and more accurately; necessary conditions are provided for controlling the larva breeding density in the next step; because the culex ovulatory valve can not be preserved, the female culex can be hatched quickly after spawning; the method is helpful for improving the growth synchronism of larvae. The method is simple to operate, low in cost and beneficial to large-scale production and use.
The above description is for the purpose of illustrating embodiments of the invention and is not intended to limit the invention, and it will be apparent to those skilled in the art that any modification, equivalent replacement, or improvement made without departing from the spirit and principle of the invention shall fall within the protection scope of the invention.
Claims (7)
1. A culex oviposition and hatching method is characterized by comprising the following steps:
early preparation: feeding blood to the emerged female culex mosquitoes in a mosquito cage, and waiting for the ovary development of the female culex mosquitoes;
spawning for the first time: placing the spawning cup into a mosquito cage, enabling culex female mosquitoes to spawn in the spawning cup, and taking out the spawning cup after 24 hours; the inner wall of the spawning cup is tightly attached to a filter screen, and the water surface in the spawning cup exceeds the bottom of the filter screen;
hatching: hatching the egg valve produced by female culex mosquitoes in the egg laying cup; after 24 hours, removing the filter screen, and putting the unhatched eggs left on the filter screen into another egg laying cup for further incubation for 24 hours;
and (3) spawning and hatching for the second time: feeding blood to female culex in the mosquito cage after the female culex spawns for the first time, putting the spawning cup into the mosquito cage, and taking out the spawning cup after 24 hours; repeating the incubating step.
2. The method for culex oviposition and hatching according to claim 1, wherein in the preliminary preparation step, the female culex mosquitoes are fed with blood for 3 days.
3. The method for culex mosquitos spawning and hatching according to claim 1 or 2, wherein the blood is sheep blood.
4. The culex oviposition and hatching method according to claim 1, wherein the mesh size of the filter mesh screen is 18-30 mesh.
5. The culex spawning and hatching method according to claim 1 or 4, wherein the filter mesh screen is made of a stainless steel mesh.
6. The method for culex mosquitoes to spawn and hatch according to claim 1, wherein the female culex mosquitoes are killed after completing the second spawning.
7. The method for culex oviposition and hatching according to claim 1, wherein the step requires environmental conditions of a temperature of 26 ± 1 ℃ and a humidity of 75 ± 10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111014010.7A CN113749056A (en) | 2021-08-31 | 2021-08-31 | Culex spawning and hatching method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111014010.7A CN113749056A (en) | 2021-08-31 | 2021-08-31 | Culex spawning and hatching method |
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| Publication Number | Publication Date |
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| CN113749056A true CN113749056A (en) | 2021-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111014010.7A Pending CN113749056A (en) | 2021-08-31 | 2021-08-31 | Culex spawning and hatching method |
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| Country | Link |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203646340U (en) * | 2013-12-13 | 2014-06-18 | 广州沃巴克生物科技有限公司 | Adult mosquito raising device |
| CN103960204A (en) * | 2014-05-30 | 2014-08-06 | 青岛农业大学 | Method for feeding lepidopteron |
| CN107372371A (en) * | 2017-09-08 | 2017-11-24 | 广州威佰昆生物科技有限公司 | Breeding method of aedes albopictus oviposition female mosquito |
-
2021
- 2021-08-31 CN CN202111014010.7A patent/CN113749056A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203646340U (en) * | 2013-12-13 | 2014-06-18 | 广州沃巴克生物科技有限公司 | Adult mosquito raising device |
| CN103960204A (en) * | 2014-05-30 | 2014-08-06 | 青岛农业大学 | Method for feeding lepidopteron |
| CN107372371A (en) * | 2017-09-08 | 2017-11-24 | 广州威佰昆生物科技有限公司 | Breeding method of aedes albopictus oviposition female mosquito |
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Application publication date: 20211207 |