CN107372371B - Breeding method of aedes albopictus oviposition female mosquito - Google Patents
Breeding method of aedes albopictus oviposition female mosquito Download PDFInfo
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- CN107372371B CN107372371B CN201710803964.3A CN201710803964A CN107372371B CN 107372371 B CN107372371 B CN 107372371B CN 201710803964 A CN201710803964 A CN 201710803964A CN 107372371 B CN107372371 B CN 107372371B
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- 241000255925 Diptera Species 0.000 title claims abstract description 149
- 241000256173 Aedes albopictus Species 0.000 title claims abstract description 62
- 230000017448 oviposition Effects 0.000 title claims abstract description 36
- 238000009395 breeding Methods 0.000 title abstract description 19
- 235000013601 eggs Nutrition 0.000 claims abstract description 50
- 230000012447 hatching Effects 0.000 claims abstract description 47
- 238000000034 method Methods 0.000 claims abstract description 26
- 230000000384 rearing effect Effects 0.000 claims abstract description 21
- 239000008280 blood Substances 0.000 claims description 23
- 210000004369 blood Anatomy 0.000 claims description 23
- 238000011534 incubation Methods 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 17
- 239000001888 Peptone Substances 0.000 claims description 16
- 108010080698 Peptones Proteins 0.000 claims description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 16
- 235000019319 peptone Nutrition 0.000 claims description 16
- 239000007864 aqueous solution Substances 0.000 claims description 13
- 230000032669 eclosion Effects 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 241001494479 Pecora Species 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 241000382353 Pupa Species 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 13
- 241000238631 Hexapoda Species 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 6
- SPFMQWBKVUQXJV-BTVCFUMJSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;hydrate Chemical compound O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O SPFMQWBKVUQXJV-BTVCFUMJSA-N 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000001490 Dengue Diseases 0.000 description 2
- 206010012310 Dengue fever Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 208000025729 dengue disease Diseases 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to a method for feeding aedes albopictus oviposition female mosquitoes, which comprises the following steps: the mosquito pupae of the aedes albopictus are placed into adult mosquito rearing cages to enable the mosquito pupae to emerge into adult mosquitoes, and the number ratio of female aedes albopictus pupae to male aedes albopictus pupae placed into each adult mosquito rearing cage is 1-5: 1. The breeding method can obtain higher spawning rate, the obtained mosquito eggs have high quality, the larva hatching rate is high (especially when the male-female ratio is 3:1, the spawning amount of the female mosquito is the highest), the spawning efficiency of the aedes albopictus is greatly improved, and the breeding method can be used as a standard spawning female mosquito breeding method, provides powerful guarantee for large-scale production of the aedes albopictus in laboratories and factories, and solves the problem of insufficient egg supply in the production process of male aedes albopictus release.
Description
Technical Field
The invention relates to the technical field of insect breeding, in particular to a breeding method of egg-laying female mosquitoes of aedes albopictus.
Background
The artificial feeding of insects is one of the basic techniques for studying entomology, and can breed certain kinds of target insects without season limitation, so that the artificial feeding of insects can be used for studying the aspects of insect biology, physiology, comprehensive pest control measure preparation and the like. Aedes albopictus is the most main transmission medium of dengue fever viruses in mainland areas of China and has wide distribution latitude. The best method for controlling the dengue fever epidemic at present is to control the density of Aedes albopictus, wherein the population density of the wild Aedes albopictus can be greatly reduced by releasing male mosquitoes carrying Volbachira symbiotic bacteria as a biological control method; therefore, the method for artificially feeding aedes albopictus in large scale is urgently needed; firstly, a standard breeding method for spawning female mosquitoes needs to be established, and related research reports about the spawning and breeding methods of the mosquitoes are fresh at home and abroad. The raising efficiency, the egg laying rate and the egg laying quality of the aedes albopictus female mosquito are difficult to improve according to other insect raising methods, so that the problem of insufficient egg supply in the production process of male aedes albopictus release can be caused, and the insect sterile technology cannot be popularized on a large scale to prevent and control the aedes albopictus.
Disclosure of Invention
Based on the method, the invention provides a method for breeding the aedes albopictus female mosquitoes, and the breeding method has higher female mosquito egg laying rate and egg laying quality.
The specific technical scheme is as follows:
a method for feeding aedes albopictus spawning female mosquitoes comprises the following steps: the mosquito pupae of the aedes albopictus are placed into adult mosquito rearing cages to enable the mosquito pupae to emerge into adult mosquitoes, and the number ratio of female aedes albopictus pupae to male aedes albopictus pupae placed into each adult mosquito rearing cage is 1-5: 1.
Preferably, the number ratio of female white-line aedes albopictus pupae to male white-line aedes albopictus pupae in each adult mosquito rearing cage is 2-4: 1.
Preferably, the number ratio of the female white-line aedes albopictus pupae to the male white-line aedes albopictus pupae is 2.8-3.2: 1.
Preferably, the female pupae of aedes albopictus and the male pupae of aedes albopictus are put into each adult mosquito rearing cage in a number ratio of 3: 1.
Preferably, the breeding method of the aedes albopictus egg-laying female mosquito comprises the following steps:
eclosion: placing the female pupae of the aedes albopictus and the male pupae of the aedes albopictus into an adult mosquito rearing cage according to the quantity ratio, so that the mosquito pupae eclosion is adult mosquitoes, providing a glucose solution for the adult mosquitoes during the eclosion period, and the eclosion time is 2-3 days;
spawning: feeding blood to female mosquitoes after 4-6 days after the mosquito pupae are completely eclosized into adult mosquitoes, placing an egg laying cup in the adult mosquito feeding cage after the blood is fed, allowing the female mosquitoes to lay eggs, and collecting the eggs in the egg laying cup after the eggs are laid;
hatching: after the mosquito eggs develop for 6-8 days, the mosquito eggs are hatched into larvae.
Preferably, the spawning comprises: feeding blood to female mosquitoes for the first time after 4-6 days after the mosquito pupae are completely eclosion into adult mosquitoes;
after one day after the first blood feeding, placing a spawning cup in the adult mosquito rearing cage until the female mosquito spawns for the first time;
collecting the mosquito eggs in the egg laying cup for the first egg laying after two days, and feeding the female mosquitoes with blood for the second time;
after the second blood feeding, placing a spawning cup in the adult mosquito rearing cage for the female mosquito to spawn for the second time;
two days later, the mosquito eggs in the egg laying cup for the second time are collected.
Preferably, the blood feeding comprises feeding anticoagulated sheep blood, the temperature of the anticoagulated sheep blood is 35-40 ℃, and the time of each blood feeding is 1-2 hours.
Preferably, the method of incubating mosquito eggs into larvae comprises the steps of:
fermenting the hatching fluid for hatching the mosquito eggs to obtain fermented hatching fluid; the hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of the yeast peptone in the aqueous solution is 0.7-1.5 g/L;
weighing mosquito eggs and adding the mosquito eggs into an incubation tube, adding the fermented incubation liquid into the incubation tube, sealing a tube cover of the incubation tube, shaking uniformly, standing for incubation, opening the tube cover of the incubation tube after incubation is finished, and transferring the incubated larvae into a feeding tray.
Preferably, the hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of the yeast peptone in the aqueous solution is 0.8-1.0 g/L.
Preferably, the hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of the yeast peptone in the aqueous solution is 0.9 g/L.
Preferably, the yeast peptone is Angel yeast peptone.
Preferably, the temperature of the fermentation is 25-30 ℃, and the time of the fermentation is 20-28 hours.
Preferably, the addition amount of the hatching fluid after fermentation is 50-80% of the volume of the hatching tube.
Preferably, the addition amount of the hatching fluid after fermentation is 60-70% of the volume of the hatching tube.
Preferably, the ratio of the mosquito eggs to the fermented hatching fluid is 1 g: 150 and 350 mL.
Preferably, the temperature of the standing incubation is 25-30 ℃, and the time of the standing incubation is 0.8-1.2 hours.
Preferably, the glucose aqueous solution has a mass concentration of 8-12%.
Preferably, the adult mosquito rearing cage is arranged in an adult mosquito rearing room, the temperature of the adult mosquito rearing room is 26-28 ℃, and the relative humidity is 65-85%.
The breeding method of the aedes albopictus spawning female mosquito has the following advantages and beneficial effects:
the inventor of the present invention has unexpectedly found out in the long-term experience accumulation and experimental process that when breeding female mosquitoes of aedes albopictus for laying eggs, male and female mosquitoes of mosquito pupae play a key role in comparison with the breeding effect, egg laying rate and egg laying quality of the female mosquitoes. By feeding the mosquito pupae with the male-female ratio, higher spawning rate can be obtained, the obtained mosquito eggs are high in quality, the larva hatching rate is high (especially when the male-female ratio is 3:1, the spawning amount of the female mosquito is highest), and the spawning efficiency of the aedes albopictus is greatly improved.
The breeding method provided by the invention can ensure the excellent egg laying amount and egg laying quality of the aedes albopictus, can be used as a breeding method of standardized female mosquitoes for egg laying, provides powerful guarantee for large-scale production of aedes albopictus in laboratories and factories, and solves the problem of insufficient egg supply in the production process of male aedes albopictus release.
In the breeding method, the water solution prepared by yeast peptone with a specific proportion is preferably selected as the hatching solution in the mosquito egg hatching step, so that the hatching rate of the aedes albopictus eggs can be greatly increased, the hatching rate of active mosquito eggs in 1 hour is high, and the larva growth synchronism is good. The method has no strict requirement on the tightness of the hatching tube, a certain amount of oxygen is reserved in the hatching tube to be more favorable for the hatching effect, the hatched larvae cannot die due to oxygen deficiency in a short time, the survival rate of the larvae is high, and a prerequisite is created for further large-scale standardized feeding of aedes albopictus.
Detailed Description
The method for breeding the female mosquito that spawns of aedes albopictus according to the present invention will be described in further detail with reference to the following embodiments.
Example 1
The method for feeding aedes albopictus spawning female mosquitoes provided by the embodiment comprises the following steps:
eclosion: in an adult mosquito rearing room with a temperature of 26-28 ℃ and a relative humidity of 65-85%, mosquito pupae of aedes albopictus, of which the number ratio of female pupae to male pupae of aedes albopictus is shown in table 1, are placed into an adult mosquito rearing cage (specification: 30cm), a box cover of a pupae box in the adult mosquito rearing cage is opened, the mosquito pupae is turned into adult mosquitoes, and a glucose water solution with the mass concentration of 10% is provided for the adult mosquitoes during eclosion (the method is that 10% of cotton soaked by the glucose water solution is placed above the adult mosquito rearing cage to ensure that the cotton contains enough glucose water solution, and the cotton containing the glucose water solution is changed once every other day), and the mosquito pupae are totally eclosion into adult mosquitoes after 2 days. Three parallel sample experiments were performed for each ratio.
Spawning: after 5 days, feeding anticoagulated sheep blood for the first time to the female mosquitoes (the temperature of the sheep blood is maintained at about 37 ℃ so as to be convenient for the female mosquitoes to suck, and the blood feeding time is about 1-2 hours each time), after one day, placing a spawning cup in a adult mosquito feeding cage until the female mosquitoes spawn for the first time; collecting the mosquito eggs in the egg laying cup for the first egg laying after two days, and feeding anticoagulated goat blood for the second time for the female mosquitoes; after the second blood feeding, placing a spawning cup in the adult mosquito rearing cage for the female mosquito to spawn for the second time; two days later, the mosquito eggs in the egg laying cup for the second egg laying were collected, and the number of eggs laid twice was counted as shown in table 1.
Hatching: the mosquito eggs can grow to be mature after being collected for one week, and the mature mosquito eggs are hatched into larvae, and the method comprises the following steps: adding water into yeast peptone to prepare an aqueous solution with the concentration of 0.9g/L, and taking the aqueous solution as hatching fluid; the yeast peptone is Angel yeast peptone FP101, which is a stable, safe and comprehensive nutrient peptone prepared by separating and enriching yeast protein treatment and complex enzyme action of pure cultured high-protein baker's yeast. The contents thereof are as follows, total nitrogen: not less than 10.0%, a-amino nitrogen: not less than 2.5%, water content: less than or equal to 6.0 percent, NaCl: 2.0% or less, ash content: less than or equal to 15.0%, pH (2% solution): 5.3-7.2;
placing the hatching solution at the temperature of 25-30 ℃ for fermenting for 24 hours to obtain fermented hatching solution;
weighing 0.05g of aedes albopictus eggs (1g ═ 15 ten thousand eggs) and adding to a 15mL hatching tube, and adding 10mL of fermented hatching fluid (the fermented hatching fluid accounts for 2/3 of the volume of the hatching tube) to the hatching tube; sealing the tube cover of the incubation tube, shaking up, standing and incubating for 1 hour at the temperature of 25-30 ℃, then opening the tube cover of the incubation tube, and transferring the incubated larvae to a feeding tray. The larval hatchability of the mosquito eggs was counted as shown in table 1. Wherein, the larva hatchability is the ratio of the number of hatched larvae to the total number of hatched mosquito eggs, and the higher the hatchability is, the higher the quality of the mosquito eggs is, and the higher the proportion of active mosquito eggs is. The hatching rate of the active mosquito eggs refers to the ratio of the number of hatched larvae to the total number of the active mosquito eggs used for hatching.
TABLE 1
As can be seen from the results in table 1: the male and female ratio of the mosquito pupae plays a key role in the breeding rate and the quality of spawning of the female mosquitoes. When the male-female ratio is 2: 1-4: 1, the average egg laying amount of a single female mosquito is 57.15-54.98, the average hatching rate of larvae is 68.58-71.90%, and particularly when the male-female ratio is 3:1, the egg laying amount of the female mosquito is the highest, the mosquito egg quality is high, and the larva hatching rate is high; when the ratio of the male to the female exceeds the range of 1: 1-5: 1, the egg laying amount or the hatching rate of the mosquitoes is greatly reduced, and when the ratio of the male to the female is 1:2, the average egg laying amount of a single female mosquito is 42.32; when the male-female ratio is 6:1, the average egg laying amount of a single female mosquito is 40.13, and the average hatching rate of larvae is 53.43 percent. It can be seen that the preferred range of the hermaphroditic ratio is 2:1 to 4:1, and controlling the hermaphroditic ratio within this range enables both higher egg laying rate and larva hatching rate to be achieved.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (9)
1. A method for raising aedes albopictus spawning female mosquitoes is characterized by comprising the following steps: placing mosquito pupas of aedes albopictus into adult mosquito rearing cages to ensure that the mosquito pupas eclose into adult mosquitoes, and placing female aedes albopictus pupas and male aedes albopictus pupas into each adult mosquito rearing cage in a quantity ratio of 2.8-3.2: 1; also comprises oviposition, and hatching the mosquito eggs into larvae after the mosquito eggs develop for 6-8 days; the process of hatching the mosquito eggs into larvae comprises the following steps:
fermenting the hatching fluid for hatching the mosquito eggs to obtain fermented hatching fluid; the hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of the yeast peptone in the aqueous solution is 0.7-1.5 g/L; the fermentation temperature is 25-30 ℃, and the fermentation time is 20-28 hours;
weighing mosquito eggs and adding the mosquito eggs into an incubation tube, adding the fermented incubation liquid into the incubation tube, sealing a tube cover of the incubation tube, shaking uniformly, standing for incubation, opening the tube cover of the incubation tube after incubation is finished, and transferring the incubated larvae into a feeding tray.
2. The method of feeding aedes albopictus spawning female mosquitoes according to claim 1, wherein the number ratio of aedes albopictus female pupae to aedes albopictus male pupae is 3:1 in each adult mosquito feeding cage.
3. The method for raising an aedes albopictus spawning female mosquito according to any one of claims 1-2, comprising the steps of:
eclosion: the female and male Aedes albopictus pupae in a ratio of the number to the number of the male Aedes albopictus pupae of any one of claims 1-2, wherein the female and male Aedes albopictus pupae are placed in an adult mosquito cage to allow the Aedes albopictus pupae to emerge as adult mosquitoes, and the adult mosquitoes are supplied with an aqueous glucose solution during the emergence for 2-3 days;
spawning: feeding blood to female mosquitoes after 4-6 days after the mosquito pupae are completely eclosized into adult mosquitoes, placing an egg laying cup in the adult mosquito feeding cage after the blood is fed, allowing the female mosquitoes to lay eggs, and collecting the eggs in the egg laying cup after the eggs are laid;
hatching: after the mosquito eggs develop for 6-8 days, the mosquito eggs are hatched into larvae.
4. The method of raising an aedes albopictus egg-laying female mosquito according to claim 3, wherein the laying eggs comprises: feeding blood to female mosquitoes for the first time after 4-6 days after the mosquito pupae are completely eclosion into adult mosquitoes;
after one day after the first blood feeding, placing a spawning cup in the adult mosquito rearing cage until the female mosquito spawns for the first time;
collecting the mosquito eggs in the egg laying cup for the first egg laying after two days, and feeding the female mosquitoes with blood for the second time;
after the second blood feeding, placing a spawning cup in the adult mosquito rearing cage for the female mosquito to spawn for the second time;
two days later, the mosquito eggs in the egg laying cup for the second time are collected.
5. The method of feeding aedes albopictus spawning female mosquitoes according to claim 3, wherein the feeding comprises feeding anticoagulated sheep blood, the anticoagulated sheep blood is at a temperature of 35-40 ℃ and the time of each feeding is 1-2 hours.
6. The method for feeding aedes albopictus spawning female mosquitoes according to claim 1, wherein the concentration of yeast peptone in the aqueous solution is 0.8-1.0 g/L.
7. The method for feeding aedes albopictus spawning female mosquitoes according to claim 1 or 6, wherein the addition amount of the hatching solution after fermentation is 50-80% of the volume of the hatching tube, and/or the ratio of the mosquito eggs to the hatching solution after fermentation is 1 g: 150 and 350 mL.
8. The method for feeding aedes albopictus spawning female mosquitoes according to claim 3, wherein the concentration of the glucose aqueous solution is 8-12% by mass.
9. The method for raising aedes albopictus spawning female mosquitoes according to claim 3, wherein the adult mosquito raising cage is placed in an adult mosquito raising chamber, the adult mosquito raising chamber has a temperature of 26-28 ℃ and a relative humidity of 65-85%.
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| CN113749056A (en) * | 2021-08-31 | 2021-12-07 | 广州威佰昆生物科技有限公司 | Culex spawning and hatching method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103155906A (en) * | 2013-04-02 | 2013-06-19 | 北京市农林科学院 | Aphidoletes aphidimyza mass reproduction technology and application technology |
| CN106259211A (en) * | 2016-08-11 | 2017-01-04 | 广州威佰昆生物科技有限公司 | Method for sterilizing female mosquitoes infected with Wolbachia albopictus |
| CN107018956A (en) * | 2017-03-20 | 2017-08-08 | 广州威佰昆生物科技有限公司 | Standardized breeding method for aedes albopictus larvae |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155906A (en) * | 2013-04-02 | 2013-06-19 | 北京市农林科学院 | Aphidoletes aphidimyza mass reproduction technology and application technology |
| CN106259211A (en) * | 2016-08-11 | 2017-01-04 | 广州威佰昆生物科技有限公司 | Method for sterilizing female mosquitoes infected with Wolbachia albopictus |
| CN107018956A (en) * | 2017-03-20 | 2017-08-08 | 广州威佰昆生物科技有限公司 | Standardized breeding method for aedes albopictus larvae |
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