CN111011312A - Device and method for obtaining various insect states of walnut leaf lifting moths in batches - Google Patents
Device and method for obtaining various insect states of walnut leaf lifting moths in batches Download PDFInfo
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- CN111011312A CN111011312A CN201911366558.0A CN201911366558A CN111011312A CN 111011312 A CN111011312 A CN 111011312A CN 201911366558 A CN201911366558 A CN 201911366558A CN 111011312 A CN111011312 A CN 111011312A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention relates to a device and a method for obtaining various insect states of walnut moth lifting in batches, which comprises a larva collector and an adult collecting box, wherein the larva collector comprises a funnel-shaped collecting worm pipe which is wide at the top and narrow at the bottom and penetrates through the collecting worm pipe from top to bottom; the adult collecting box comprises a rectangular box body, the box body is provided with a box door with a handle, a plurality of spawning racks are arranged at the bottom in the box body, each spawning rack comprises a supporting vertical rod, and a walnut bracket is arranged at the top of each supporting vertical rod. The device has simple structure, improves the eclosion rate and the egg laying amount of the imagoes while greatly reducing the time, can greatly reduce the time, improves the eclosion rate and the egg laying amount of the imagoes, and has better application prospect.
Description
Technical Field
The invention relates to a device and a method for obtaining various insect states of walnut shell worms in batches.
Background
The walnut shell worms Atrijuglans hetaohei Yang belong to Lepidoptera shell mothidae, are main pests on walnuts, occur in various walnut producing areas in China, and eat walnut fruits and kernels by larvae. The damaged fruit skin is blackened and shriveled, and the kernels are dried and darkened, so that early fruit drop is caused, the yield and the commodity value of the walnuts are seriously influenced, and the damaged fruit skin is also named as 'walnut black' and 'black walnuts'. In recent years, with the expansion of walnut cultivation area and the introduction of new varieties, the walnut limb moth is commonly generated in each production area, the damage is gradually increased, the fruit damage rate is usually up to 70% -80%, even up to 100%, and the serious yield reduction is caused. Because the insect is damaged when entering the fruit, the difficulty of preventing and controlling the pesticide is increased, and the comprehensive measuring and reporting technology is required to be applied in the production. Besides, the drug resistance of the walnut limb moth to the medicament, the indoor toxicity test of the medicament and the research in the aspect of molecular biology need to be researched indoors. Therefore, batch of insect states are required to be used as test objects in both walnut limb moth field and indoor research. At present, most of the original sources of the walnut limb lifting moths for the test are field catching, field egg collecting and field summer cocoon overwintering cocoons digging, but the methods mainly have the following problems:
(1) enabling the walnut limb moth to generate 1-2 generations in one year, enabling female worms to lay eggs on fruit surfaces in the emergence period of the walnut limb moth adults, and crawling for several minutes after hatching to eat fruits; because the time from the larva hatching to the fruit-eating is short, great difficulty is brought to catching, and even a small amount of larvae are caught, the larvae are difficult to be used for developing research experiments due to inconsistent instar periods;
(2) the walnut limb moth overwinter with the mature larvae in soil, the overwinter depth can be on the soil surface, and the deepest depth can reach more than 10cm, but the obtained amount of manually dug larvae is small, so that the later-period test requirements cannot be met. The method of collecting the larvae by using a conventional plastic box or smashing the walnuts by using an artificial axe is adopted by the applicant for two years, so that the death rate of the obtained larvae is high, the emergence rate of adults is low, the egg laying amount of the adults is low, and the ideal amount of the adults is not obtained; secondly, because of the inconsistency of the fruit-eating period, the eclosion, the oviposition and the growth and the development of the collected mature larva are irregular, the physiological state is inconsistent, a better test sample cannot be formed, the research result is greatly influenced, and the significance of the research result is lost.
Therefore, the difficulty of obtaining larvae in batches from the field or collecting the larvae by a conventional method and effectively laying eggs is high, and the obtained larvae have the problem of large individual difference and are not beneficial to later-stage research, so that a method for effectively and comprehensively preventing and treating the walnut limb lifting moths is urgently needed, wherein the method can obtain all insect states in batches, is uniform in development and has consistent physiological state.
Disclosure of Invention
In order to solve the technical defects, the invention provides a device and a method for obtaining various insect states of the walnut shell worms in batches, which can greatly reduce the time and improve the eclosion rate and the egg laying amount of adults.
The invention is realized by the following measures:
a device for obtaining each insect state of walnut moth lifting in batches comprises a larva collector and an adult collecting box, wherein the larva collector comprises a funnel-shaped collecting worm pipe which is wide at the upper part and narrow at the lower part and penetrates through the upper part and the lower part, a cylindrical bracket is arranged on the outer side of the collecting worm pipe, the collecting worm pipe is vertically placed in the bracket, a top plate is arranged at an opening at the top of the worm pipe, a plurality of worm falling holes are formed in the top plate, and an insect receiving dish is connected below an opening at the bottom of the collecting worm pipe; adult collecting box includes rectangular box, the box is provided with the chamber door of taking the handle, and the bottom is provided with a plurality of egg racks in the box, the egg rack is including supporting the montant, it is provided with the walnut bracket to support the montant top.
The diameter of the upper end of the collecting worm tube is 40-50 cm, the diameter of the lower end of the collecting worm tube is 8-10 cm, the height of the collecting worm tube is 25-30 cm, the height of the support is 20-25 cm, and the diameter of the support is 30-40 cm.
The cover is buckled above the top of the collecting worm pipe, a plurality of exhaust holes are formed in the cover and are 40-60 meshes, and the diameter of the worm falling hole is 1-2 cm.
The diameter of the bottom opening of the collecting worm tube is the same as that of the top opening of the worm receiving dish, and the bottom opening of the collecting worm tube is connected with the top opening of the worm receiving dish through a frosted interface.
The length, the width and the height of the box body of the adult collecting box are respectively 50-60 cm, 40-50 cm and 20-30 cm, the height of the spawning rack is 8-10 cm, the walnut brackets are V-shaped, the opening angle is 90 degrees, and the spawning racks are uniformly arranged in the adult collecting box and are arranged into 2-3 rows and 4-5 rows.
The sawdust which is sterilized and has the total water content kept at 5-10% is placed in the insect receiving dish, and the insect collecting pipe and the box body are made of transparent plastic materials.
A method for obtaining insect states of walnut shell worms in batches comprises the following steps:
disinfecting walnuts which are damaged by walnuts and lifted by moths and do not have fruits in the step 1 by using 200-300 ppm of sodium hypochlorite; in the steps 1, 2, 3 and 4, various devices are placed under the conditions of the temperature of 22 +/-1 ℃ and the humidity of 60 +/-10%, and the photoperiod is 12h:12 h.
The sawdust sterilized in the step 1 is sawdust subjected to high-temperature sterilization at 120 ℃, walnuts which are collected in the step 3 and do not have diseases and insect pests are walnuts which are currently used and subjected to surface disinfection treatment by using 200-300 ppm of sodium hypochlorite.
And 4, after the same batch of walnut adult moths are laid eggs, cleaning adult corpses in the adult collecting box, respectively sterilizing with sodium hypochlorite of 200-300 ppm, and washing with clear water for 2-3 times for later use.
The invention has the beneficial effects that: the device disclosed by the invention is simple in structure, the time is greatly reduced, the eclosion rate and the egg laying amount of the adult are improved, the time is saved by 29.6-59.4 times compared with the conventional plastic box collection and manual walnut smashing collection, the eclosion rate of the adult is improved by 1.31-1.99 times, and the egg laying amount is improved by 1.55-5.72 times. 2. The device and the method can replace a conventional plastic box for collecting the larvae, manually smashing the walnuts for collecting the larvae and a conventional mode for laying eggs, and solve the problems that the larvae collected by the plastic box and the manually smashed walnuts for collecting the larvae damage the aged larvae, and are not beneficial to eclosion and yield of adults. Can greatly reduce time, improve the eclosion rate and the egg laying amount of the imagoes and has better application prospect.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a schematic view of the structure of the collecting worm tube.
Fig. 3 is a schematic structural diagram of an adult collecting box.
FIG. 4 is a schematic diagram of the structure of the spawning rack.
FIG. 5 is a schematic view of the whole structure of the adult collecting box with the insect receiving dish.
Wherein, 1, a bracket; 2. collecting worm tubes; 3. a cover; 4. inoculating an insect vessel; 5. an exhaust hole; 8. a bug falling hole; 9. an adult collecting box; 10. an oviposition rack; 11. a handle; 12. a box door; 13. walnut.
Detailed Description
The invention is described in further detail below with reference to the accompanying drawings:
example 1: as shown in fig. 1, 2, 3, 4 and 5, the device for obtaining each insect state of the walnut moth lifting in batches comprises a larva collector and an adult collecting box 9, wherein the larva collector comprises a funnel-shaped collecting worm pipe 2 which is wide at the upper part and narrow at the lower part and penetrates through the collecting worm pipe from top to bottom, a cylindrical bracket 1 is arranged outside the collecting worm pipe 2, the collecting worm pipe 2 is vertically placed in the bracket 1, a top plate is arranged at an opening at the top of the worm pipe, a plurality of worm falling holes 8 are formed in the top plate, and an insect receiving vessel 4 is received below an opening at the bottom of the collecting worm pipe 2; adult collecting box 9 includes rectangular box, and the box is provided with chamber door 12 of taking handle 11, and the bottom is provided with a plurality of egg laying frame 10 in the box, and egg laying frame 10 is including supporting the montant, it is provided with the walnut bracket to support the montant top. The support 1 is made of iron materials and comprises circular iron rings at two ends and three supporting columns, and the three supporting columns are welded with the circular iron rings in a trisection mode.
The diameter of the upper end of the collecting worm tube 2 is 40-50 cm, the diameter of the lower end of the collecting worm tube is 8-10 cm, the height of the collecting worm tube is 25-30 cm, the height of the support 1 is 20-25 cm, and the diameter of the circular iron ring is 30-40 cm. Collect 2 top locks of worm pipe and have lid 3, be provided with a plurality of exhaust holes 5 on the lid 3, exhaust hole 5 is 40 ~ 60 meshes, and 8 diameters in the insect hole that falls are 1 ~ 2 cm. The diameter of the bottom opening of the collecting worm tube 2 is the same as that of the top opening of the worm receiving dish 4, and the bottom opening of the collecting worm tube 2 is connected with the top opening of the worm receiving dish 4 through a frosted interface. The length, the width and the height of the adult collecting box 9 are respectively 50-60 cm, 40-50 cm and 20-30 cm, the height of the spawning rack 10 is 8-10 cm, the walnut bracket is V-shaped, the opening angle is 90 degrees, and the spawning racks 10 are uniformly arranged in the adult collecting box 9 and are arranged in 2-3 rows and 4-5 rows. Sawdust which is sterilized and has the total water content kept at 5-10% is placed in the insect receiving dish 4, and the insect collecting tube 2 and the box body are made of transparent plastic materials.
The collecting worm tube 2 is made of transparent polyethylene, polypropylene, polymethacrylate, polyethylene terephthalate, polycarbonate, polystyrene and other types of plastic materials, the thickness of the collecting worm tube is 1-2 mm, the collecting worm tube can bear walnuts, and the fruiting situation of the walnut-lifting moth mature larvae without fruiting can be conveniently observed. The upper end of the collecting worm tube 2 is provided with a worm dropping hole 8, the diameter of the small hole is 1-2 cm, and the walnut kernel lifting moth mature larva which is convenient for fruit removal falls into the worm receiving dish 4 through the small hole.
The diameter of the upper end of the collecting worm tube 2 is 40-50 cm, and the diameter of the collecting worm tube 2 is larger than that of the circular iron ring, so that the collecting worm tube 2 can be placed on the circular support 1. The height of the collecting worm tube 2 is 25-30 cm, the collecting worm tube is too low to be placed on the circular support 1, and the collecting worm tube is too high to be used for survival of fallen walnut lifting moth mature larvae.
Collect 2 tops of worm pipe for poroid circular lid 3, its hole is exhaust hole 5, and the size in hole is 40 ~ 60 meshes, and the diameter of poroid circular lid 3 is the same with the 2 upper ends diameters of collecting worm pipe, can cover completely and collect 2 upper ends of worm pipe, prevents other small insects again, for example insects such as fruit flies get into in collecting worm pipe 2. The diameter of the lower end of the collecting worm tube 2 is 8-10 cm, and the collecting worm tube is connected with the worm receiving dish 4 through a frosted interface. The diameter of the insect receiving dish 4 is the same as that of the lower end of the collecting worm tube 2, and the walnut lifting armyworm mature larvae with fruits removed through the collecting worm tube 2 smoothly enter the insect receiving dish 4 for collection. And (3) placing sterilized sawdust in the insect receiving dish 4, and adding a proper amount of water to keep the total water content at 5-10%, so as to be beneficial to the survival of the mature larvae of the walnut shell worms and to ensure that the mature larvae eclosion into imagoes after pupation. The adult collecting box 9 is made of transparent polyethylene, polypropylene, polymethacrylate, polyethylene terephthalate, polycarbonate, polystyrene and other types of plastic materials, the thickness is 1-2 mm, and the design is convenient for observing the emergence and oviposition processes of the walnut limb lifting moths. The length, width and height of the adult collecting box 9 are 50-60 cm, 40-50 cm and 20-30 cm respectively. The size range is favorable for the adult walnuts to lay eggs after mating. One side of the adult collecting box 9 is provided with a sliding door structure, and a handle 11 is arranged on the sliding door, so that newly collected walnuts without plant diseases and insect pests can be conveniently stored and taken in the insect receiving dish 4 and placed on the egg laying frame 10.
The lower end of the spawning rack 10 is connected with the bottom of the adult collecting box 9 and stands in the adult collecting box 9. Spawning rack 10 is 8 ~ 10cm high, and the upper portion is opened the angle and is 90, and align to grid is in adult collecting box 9, 2 ~ 3 rows and 4 ~ 5 rows, and every spawning rack 10 all plays the effect of supporting a walnut weight. Newly-collected walnuts without diseases and insect pests are placed above the egg laying frame 10, and the calyx positions of the walnuts are upward, so that the adult walnuts with limb moths can lay eggs conveniently.
The method for collecting the mature larvae and the eggs by using the device for obtaining the mature larvae and the eggs of the walnuts lifted by the armyworms in batches comprises the following steps:
(1) collecting the walnuts which are damaged by the walnuts and do not shed fruits from the field.
(2) The round bracket 1, the collecting worm tube 2, the porous round cover 3 and the worm receiving dish 4 with the same model are collected. The walnut that will collect not take off the fruit is placed in the top of collecting worm pipe 2, covers poroid round cover 3, assembles the worm receiving dish 4 in the below of collecting worm pipe 2, and puts into the sawdust of sterilization in the worm receiving dish 4 to add appropriate amount of water, make the total water content keep down 5 ~ 10%, be used for collecting the mature larva.
(3) The time of collecting the mature larva is marked on the insect receiving dish 4, the mature larva in the insect receiving dish 4 is collected on the same day, the research on the mature larva can be carried out according to the test condition, or the insect receiving dish 4 is continuously put into the adult collecting box 9, and the eclosion and the oviposition of the walnut lifting moth are waited.
(4) Through the handle 11 in the adult collecting box 9, open adult collecting box 9, will collect the mature larva in the insect receiving dish 4 the same day and put into the incasement, wait for after 5 ~ 7 days, place the walnut that does not have the plant diseases and insect pests of newly gathering on every egg laying frame 10, and the calyx department of walnut is upwards, is convenient for the walnut to lift the armyworm adult and lay eggs.
(5) And taking out the walnuts laid eggs on the same day, and carrying out experimental study. And then putting another batch of newly collected walnuts without diseases and insect pests on the egg laying frame 10, and dying the eclosion adults after finishing egg laying according to the steps.
According to the invention, the walnut which is damaged by the walnut moth lifting and does not have the fruit removed in the step (1) is preferably disinfected by using 200-300 ppm of sodium hypochlorite, so that the walnut placed above the collecting worm tube 2 is prevented from being rotted due to the infection of pathogenic bacteria. In the steps (2), (3), (4) and (5), various devices are placed under the conditions of temperature of 22 +/-1 ℃ and humidity of 60% +/-10%, and the photoperiod is 12h:12 h. The sawdust sterilized in the step (2) is subjected to high-temperature sterilization at 120 ℃ to kill pathogenic organisms in the sawdust and prevent the walnut shell moth mature larvae from being damaged. The walnuts which are collected in the step (4) and do not have diseases and insect pests are the walnuts which are currently collected and are subjected to surface disinfection treatment by using 200-300 ppm of sodium hypochlorite. And (5) after the same batch of walnut adult moths are laid eggs, cleaning adult corpses in the adult collecting box 9, performing surface disinfection treatment by respectively using sodium hypochlorite of 200-300 ppm, and washing for 2-3 times by using clear water for later use.
Example 2
The device for obtaining the insect states of the walnut shell worms in batches is the same as that in the embodiment 1, except that the height of the bracket 1 is 25 cm.
Example 3
The device is the same as the device for counting entomopathogenic nematodes in example 1, except that the diameter of the upper end of the collecting worm tube 2 is 45cm
Example 4
The device for counting entomopathogenic nematodes is the same as that described in example 1, except that the length, width and height of the adult collecting box 9 are 55cm, 45cm and 25cm, respectively.
Example 5
The apparatus for counting entomopathogenic nematodes was identical to that described in example 1, except that the height of the egg laying shelf 10 was 10 cm.
Laboratory test verification
Example 6
The device for obtaining the insect states of the walnuts and the acrostichopus exigua in batch in the embodiment 1 is utilized, and the method comprises the following steps:
(1) collecting the walnuts which are damaged by the walnuts and the armyworms and are not subjected to fruit removal from the field, and disinfecting the walnuts which are not subjected to fruit removal by using 200-300 ppm of sodium hypochlorite.
(2) The device comprising the bracket 1, the worm collecting tube 2, the cover 3 and the worm receiving dish 4 is assembled. The collected walnuts without fruit peeling are placed above the collecting worm tube 2, the worm receiving dish 4 is assembled below the collecting worm tube 2 through the cover 3, sawdust subjected to high-temperature sterilization at 120 ℃ is placed in the worm receiving dish 4, a proper amount of water is added, the total water content is kept to be 5-10%, and the walnut collecting dish is used for collecting aged larvae.
(3) The time for collecting the aged larvae is marked on the insect receiving dish 4, 50 aged larvae in the insect receiving dish 4 are collected, and other experiments for observing the survival rate of the aged larvae are carried out. And (3) continuously placing the insect receiving dish 4 into the adult collecting box 9 for part of the collected mature larvae to wait for the emergence and oviposition of the walnut limb moths.
(4) And placing the aged larvae collected in the insect receiving vessel 4 in the same day into a box, waiting for 5-7 days, placing walnuts which are subjected to disinfection treatment by using 200-300 ppm of sodium hypochlorite and have no plant diseases and insect pests on each egg laying frame 10, enabling the calyx positions of the walnuts to be upward, and enabling the adult walnuts to raise their limb moths to lay eggs.
(5) And (4) taking out the walnuts spawned on the same day, and carrying out an egg hatching test.
(6) The above steps are all tested under the conditions of temperature 22 +/-1 ℃ and humidity 60% +/-10% and the photoperiod of 12h:12 h.
Comparative example 1
The same walnuts as in example 6 were selected and collected from the field as those damaged by the walnuts and the armyworm without fruit drop. The difference is that the conventional collection method is adopted, namely, the collected walnuts are placed in a plastic box, 50 larvas are manually picked up and removed, and the emergence rate and the time are calculated.
Comparative example 2
The same walnuts as in example 6 were selected and collected from the field as those damaged by the walnuts and the armyworm without fruit drop. The difference is that the method of manually smashing walnuts by using an axe is adopted to collect larvae, and the eclosion rate and the time spent are calculated.
Comparative example 3
50 head of aged larvae were collected by the same method as in comparative example 1. The method is characterized in that the method is placed in a general insect breeding net, the same number of fresh walnuts as that in the embodiment 7 are placed in the insect breeding net, the fresh walnuts are flatly placed in the insect breeding net, and the number of eggs laid on all the fresh walnuts in one day is calculated.
Comparative example 4
50 head of aged larvae were collected by the same method as in comparative example 2. The method is characterized in that the method is placed in a general insect breeding net, fresh walnuts with the same quantity as that in the embodiment 7 are placed in the insect breeding net, the fresh walnuts are flatly placed in the insect breeding net, and the number of eggs laid on all the fresh walnuts in one day is calculated.
Comparative example 5
50 mature larvae were collected by the same method as in example 6. The method is characterized in that the method is placed in a general insect breeding net, fresh walnuts with the same quantity as that in the embodiment 7 are placed in the insect breeding net, the fresh walnuts are flatly placed in the insect breeding net, and the number of eggs laid on all the fresh walnuts in one day is calculated.
Test examples
The test was conducted with 10 treatments, using the apparatus of the present invention and the conventional method (example 1, example 4, example 3, example 4 and example 5, comparative example 1, comparative example 2, comparative example 3, comparative example 4 and comparative example 5), respectively. Procedure the procedures described in example 1, example 2, example 3, example 4, example 5 and comparative example 1, comparative example 2, comparative example 3, comparative example 4 and comparative example 5 were used respectively, each treatment was repeated 3 times and the time (S) spent, larval mortality and number of eggs laid were recorded, the specific conditions and results are shown in table 1.
TABLE 1 comparison of treatment methods and Experimental results
As can be seen from Table 1, the feathering rates of examples 1, 2, 5 and 5 in which larvae were collected using the apparatus of the present invention were 86.67 to 89.33%, the feathering rates were 1.31 to 1.35 times and 1.94 to 1.99 times respectively higher than those of comparative example 1 (plastic box collection) and comparative example 2 (manual walnut collection), the time spent on comparative example 1 (plastic box collection) and comparative example 2 (manual walnut collection) was 29.6 to 31.9 times and 55.2 to 59.4 times respectively saved, and the egg yields of examples 3, 4 and 5 in which eggs were collected using the apparatus of the present invention were 113.33 to 116.33 pieces and the egg yields were 2.31 to 2.37 times, 5.57 to 5.72 times and 1.55 to 1.59 times respectively higher than those of comparative example 3 (conventional mode), comparative example 4 (conventional mode) and comparative example 5 (conventional mode). The device for obtaining the insect states of the walnut shell worms in batches by the device greatly reduces the time and improves the eclosion rate and the egg laying amount of adults.
The foregoing is only a preferred embodiment of this patent, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of this patent, and these modifications and substitutions should also be regarded as the protection scope of this patent.
Claims (10)
1. The utility model provides a each attitude device of limb moth is lifted to walnut of obtaining in batches which characterized in that: the collecting device comprises a larva collector and an adult collecting box, wherein the larva collector comprises a funnel-shaped collecting worm pipe which is wide at the top and narrow at the bottom and penetrates through the collecting worm pipe from top to bottom, a cylindrical bracket is arranged on the outer side of the collecting worm pipe, the collecting worm pipe is vertically placed in the bracket, a top plate is arranged at an opening at the top of the worm pipe, a plurality of worm falling holes are formed in the top plate, and an insect receiving dish is connected below an opening at the bottom of the collecting worm pipe; adult collecting box includes rectangular box, the box is provided with the chamber door of taking the handle, and the bottom is provided with a plurality of egg racks in the box, the egg rack is including supporting the montant, it is provided with the walnut bracket to support the montant top.
2. The device for obtaining various insect states of walnuts and armyworms in batches according to claim 1, is characterized in that: the diameter of the upper end of the collecting worm tube is 40-50 cm, the diameter of the lower end of the collecting worm tube is 8-10 cm, the height of the collecting worm tube is 25-30 cm, the height of the support is 20-25 cm, and the diameter of the support is 30-40 cm.
3. The device for obtaining various insect states of walnuts and armyworms in batches according to claim 1, is characterized in that: collect worm pipe top lock and have the lid, be provided with a plurality of exhaust holes on the lid, the exhaust hole is 40 ~ 60 meshes, the insect hole diameter that falls is 1 ~ 2 cm.
4. The device for obtaining various insect states of walnuts and armyworms in batches according to claim 1, is characterized in that: the diameter of the bottom opening of the collecting worm tube is the same as that of the top opening of the worm receiving dish, and the bottom opening of the collecting worm tube is connected with the top opening of the worm receiving dish through a frosted interface.
5. The device for obtaining various insect states of walnuts and armyworms in batches according to claim 1, is characterized in that: the length, the width and the height of the box body of the adult collecting box are respectively 50-60 cm, 40-50 cm and 20-30 cm, the height of the spawning rack is 8-10 cm, the walnut brackets are V-shaped, the opening angle is 90 degrees, and the spawning racks are uniformly arranged in the adult collecting box and are arranged into 2-3 rows and 4-5 rows.
6. The device for obtaining various insect states of walnuts and armyworms in batches according to claim 1, is characterized in that: the sawdust collecting device is characterized in that sawdust which is sterilized and has the total water content kept at 5-10% is placed in the insect receiving dish, and the insect collecting pipe and the box body are made of transparent plastic materials.
7. A method for obtaining insect states of walnuts and acrostichopus exigua in batches is characterized by comprising the following steps:
step 1, collecting walnuts which are damaged by walnuts and lifted by moths and do not shed, placing the collected walnuts which do not shed on a top plate above a collecting worm tube, covering a round cover, carrying an insect receiving dish below the collecting worm tube, placing sterilized sawdust into the insect receiving dish, adding a proper amount of water to keep the total water content at 5-10% and collecting mature larvae;
step 2, marking the time for collecting the mature larvae on an insect receiving vessel, carrying out research on the mature larvae collected in the insect receiving vessel on the same day according to the test condition, or continuously placing the insect receiving vessel into an adult collecting box, and waiting for eclosion and oviposition of the walnut limb lifting moth;
step 3, opening the box body, putting the aged larvae collected in the insect receiving vessel together with the insect receiving vessel into the box body on the same day, waiting for 5-7 days, placing newly collected walnuts without diseases and insect pests on each walnut bracket, enabling calyxes of the walnuts to face upwards, and enabling the adult walnuts to raise limb moths for the walnuts to lay eggs;
and 4, taking out the walnuts spawned on the same day, carrying out experimental research, putting another batch of newly-collected walnuts without plant diseases and insect pests on the walnut bracket, and dying the feathered adults after completing the spawning according to the steps.
8. The method for obtaining insect states of walnuts and acroleid moths in batches according to claim 1, which is characterized in that: disinfecting walnuts which are damaged by walnuts and lifted by moths and do not have fruits in the step 1 by using 200-300 ppm of sodium hypochlorite; in the steps 1, 2, 3 and 4, various devices are placed under the conditions of the temperature of 22 +/-1 ℃ and the humidity of 60 +/-10%, and the photoperiod is 12h:12 h.
9. The method for obtaining insect states of walnuts and acroleid moths in batches according to claim 1, which is characterized in that: the sawdust sterilized in the step 1 is sawdust subjected to high-temperature sterilization at 120 ℃, walnuts which are collected in the step 3 and do not have diseases and insect pests are walnuts which are currently used and subjected to surface disinfection treatment by using 200-300 ppm of sodium hypochlorite.
10. The method for obtaining insect states of walnuts and acroleid moths in batches according to claim 1, which is characterized in that: and 4, after the same batch of walnut adult moths are laid eggs, cleaning adult corpses in the adult collecting box, respectively sterilizing with sodium hypochlorite of 200-300 ppm, and washing with clear water for 2-3 times for later use.
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| CN115281166A (en) * | 2022-08-25 | 2022-11-04 | 云南农业大学 | Rapid detection and collection device for tomato leaf miner larvae and use method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115281166A (en) * | 2022-08-25 | 2022-11-04 | 云南农业大学 | Rapid detection and collection device for tomato leaf miner larvae and use method |
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Application publication date: 20200417 |