CN104956909A - Safe and highly-efficient scale-cutting technique for detoxified longya lily - Google Patents
Safe and highly-efficient scale-cutting technique for detoxified longya lily Download PDFInfo
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- CN104956909A CN104956909A CN201510453975.4A CN201510453975A CN104956909A CN 104956909 A CN104956909 A CN 104956909A CN 201510453975 A CN201510453975 A CN 201510453975A CN 104956909 A CN104956909 A CN 104956909A
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- scale
- cuttage
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- detoxification
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Provided is a safe and highly-efficient scale-cutting technique for detoxified longya lily. The technique is realized by taking following steps: storing seedballs of detoxified longya lily for a refrigeration house; disinfecting scales; dissecting scales; cutting dissected scales; and covering a cutting box with a black thin film after cutting operation. The dissected scales are cut in inorganic, soilless and light-media perlite for asexual reproduction. Two cormels are reproduced from each dissected scale with the reproduction coefficient up to 4 to 6. The safe and highly-efficient scale-cutting technique for detoxified longya lily has following advantages: cormels subjected to asexual reproduction can keep detoxified quality of detoxified longya lily; viruses and microbiological hazards during a cutting process are reduced; reproduction coefficient of all scales is increased; residual hormones in scales or seedballs are decreased; scales are saved; and cost is reduced.
Description
Technical field
The present invention relates to the safe and efficient scale cuttage technique of a kind of detoxification Lilium brownii var viridulum, platymiscium cuttage vegetative propagation technique field.
Background technology
Lilium brownii var viridulum, is one of local microspecies of edible lily, is also one of Jiangxi Ways of Special Agricultural Products, can eats raw, can be processed into powder, can add and make noodles etc., also can be processed into all multipurposes such as beverage.Lilium brownii var viridulum kind ball price is higher.In current production, the breeding of detoxification Lilium brownii var viridulum has 2 kinds of common methods, and full scale or full scale dip the mode such as plant hormone cuttage and the training of Lilium brownii var viridulum group.Along with ecommerce and food diversified development, Lilium brownii var viridulum kind ball and the converted products market demand potential large.Especially along with food-safety problem is more and more paid attention to, it is the first link that food security raw material is produced.There are some problems in the full scale cuttage of tradition lily, CN104663190A discloses a kind of full scale lining method of rattlebush, but the method exists the low problem of scale availability.CN101711493A discloses a kind of vegetative propagation technique directly stripping scale lining lily ball, but this method exists the problems such as reproduction coefficient is low.CN102577804A discloses a kind of method improving lily bud scale cottage propagation rate.This patent, for viewing and admiring oriental hybrid lily kind, mainly takes hot water treatment, various chemical medicaments process, adopts the measures such as organic substrate to improve its bird watching kind cottage propagation rate, and full scale cottage propagation coefficient is low, generally only has 2; Fancy breed is considered food security unlike detoxification Lilium brownii var viridulum edible lily kind and adopts physical disinfection and inorganic without measures such as native Light media perlites more.CN102577804 patent does not relate to detoxification Lilium brownii var viridulum edible variety, low temperature treatment, scale physical disinfection, inorganic without technology such as each segment scale cottage propagation seed balls after native Light media perlite, scale segment yet.
Existing plant cuttage generally all will adopt hormone, but as food Lilium brownii var viridulum, just should not adopt the HORMONE TREATMENT of normal cutting propagation.The cuttage of existing lily class plant scale generally adopts full scale cuttage, and its full scale lining coefficient generally only has 2, causes scale to be wasted, and cost increases.
Summary of the invention
The object of the invention is, there is hormone residues for current Lilium brownii var viridulum cottage propagation, scale availability is low, reproduction coefficient is low, high in cost of production problem.The invention provides the safe and efficient scale cuttage technique of a kind of detoxification Lilium brownii var viridulum.
The present invention is achieved through the following technical solutions: the inventive method comprises scale sterilization, scale segment and the cuttage of segment scale, also comprises the low temperature treatment before scale cuttage and the many segments of scale are inorganic without native Light media perlite cuttage vegetative propagation.
Described scale is disinfected, and adopts chitosan solution to soak;
Described scale many segments process, is cut into independent 3 sections along vertical long axis, i.e. 1/3 scale by skin and middle level scale; Internal layer scale is cut into independent 2 sections along vertical long axis, i.e. 1/2 scale.
A kind of detoxification Lilium brownii var viridulum of the present invention safe and efficient scale cuttage technique step is as follows:
(1) cold storage of seedball
Gather in the crops the raw cultivation in detoxification Lilium brownii var viridulum plantlet in vitro land for growing field crops 3 years commodity kind ball August, select disease-free, reach 35 g ~ 50 g detoxification Lilium brownii var viridulum kind balls without wound, quality, the kind ball with a small amount of earth tans by the sun 2 days, puts into 5 DEG C of Storage in cold banks afterwards.2 DEG C of low-temperature cold store process 2 days are needed before cuttage.
(2) scale sterilization
Disease-freely waiting detoxification Lilium brownii var viridulum kind ball without rotting after getting refrigeration, peeling scale from outside to inside gently, putting into 100 ppm shitosans and soak 3 hours.And agitating solution gently.
(3) scale segment
With sterilizing, skin and middle level scale are cut into independent 3 sections along vertical long axis, i.e. 1/3 scale by cutter; Internal layer scale is cut into independent 2 sections along vertical long axis, i.e. 1/2 scale.Under normal temperature, after shady and cool 3 hours, segment scale uviol lamp is killed virus.
(4) segment scale cuttage
Before the cuttage of segment scale, freshly inorganicly shift to an earlier date 3 days without native Light media perlite and tan by the sun, inorganic loading without native Light media perlite will be tanned by the sun and treat cuttage bubble chamber, and appropriate compacting pearlite interstitial substance, and to spray stromal surface with 50 ppm shitosans.Segment scale without the need to Plant hormone treatment, by the cuttage down of segment scale biology lower end in inorganic without native Light media pearlite interstitial substance, the cuttage degree of depth 3 cm, cuttage seeding row spacing 5 cm × 5 cm.Cuttage case surrounding leaves pore.
(5) black thin film is covered after inserting
After the cuttage of segment scale, inorganic without the moisturizing of native Light media pearlite interstitial substance surface spray, black thin film is sprayed and is hung with water droplet, covers cuttage case immediately.Observe matrix and black film humidity, take the circumstances into consideration spraying, after cuttage, plastic tunnel put into by cuttage case.
(6) seed ball is cultivated
After waiting for that segment scale biology lower end grows 2 white seed balls, namely full scale lining coefficient reaches 4 ~ 6.Releasing black thin film covers, grown cultures management under normal management condition in booth.
The invention has the beneficial effects as follows, the inventive method employing segment scale is inorganic all can breed 2 seed balls without native Light media perlite cuttage vegetative propagation, and namely each cut-out scale lining coefficient reaches 2, and full scale lining coefficient reaches 4 ~ 6; Save scale, reduce costs.
The present invention adopts shitosan soaking disinfection to scale, and thimerosal can be prevented the pollution of Lilium brownii var viridulum; Cuttage process does not adopt hormone, prevents hormone residual in scale or seed ball; Ensure the edible safety standard of Lilium brownii var viridulum.
Embodiment
The safe and efficient scale cuttage technique of a kind of detoxification Lilium brownii var viridulum of the present invention, its embodiment comprises the steps:
(1) cold storage of seedball
After detoxification in August Lilium brownii var viridulum land for growing field crops acrial part is withered, the 3 years raw cultivation commodity kind balls in results detoxification Lilium brownii var viridulum plantlet in vitro land for growing field crops, select disease-free, long and loose, quality reaches the detoxification Lilium brownii var viridulum fresh kind of balls such as 35 g ~ 50 g without wound, scale, fresh kind of ball of a small amount of earth of band is tanned by the sun 2 days, puts into about 5 DEG C Storage in cold banks afterwards.Certain space is kept between fresh kind of ball.The front 2 DEG C of freezer low temperature treatment of cuttage 2 days.
(2) scale sterilization
Select, without the low temperature treatment detoxification Lilium brownii var viridulum fresh kind of ball such as disease-free that rot, to peel scale from outside to inside gently, put into 100 ppm shitosans gently and soak 3 hours.Period agitating solution gently.
(3) scale segment
Skin and middle level scale have on average been cut into independent 3 sections along vertical long axis by high-temperature sterilization cutter is 1/3 scale (upper end segment, middle segment, lower end segment), 1/3 segment scale mean fresh 1.11 g; It is 1/2 scale (upper end segment and lower end segment) that internal layer scale is cut into independent 2 sections along vertical long axis, 1/2 segment scale mean fresh 1.66g.Under normal temperature, after shady and cool 1 hour, segment scale uviol lamp is killed virus 10 seconds.
(4) segment scale cuttage
Freshly inorganicly shift to an earlier date 3 days without native Light media perlite and tan by the sun, inorganic loading without native Light media perlite will be tanned by the sun and treat foam cuttage case, appropriate compacting pearlite interstitial substance, and to spray stromal surface with 50 ppm shitosans.Segment scale is without the need to Plant hormone treatment, and by segment scale biology lower end, cuttage is in inorganic without in native Light media pearlite interstitial substance gently down, and the cuttage degree of depth 3 cm, segment scale does not expose, seeding row spacing 5 cm × 5 cm.The design of cuttage case surrounding leaves pore.
(5) black thin film is covered after inserting
After cuttage, inorganic without the moisturizing of native Light media pearlite interstitial substance surface spray, the black thin film to be covered hang with water droplet of spraying, black film covers cuttage case immediately.Observe soil humidity and black film humidity, take the circumstances into consideration spraying, after cuttage, plastic greenhouse put into by cuttage case.
(6) seed ball is cultivated
After waiting for that segment scale biology lower end grows 2 white seed balls, namely full scale lining coefficient reaches 4 ~ 6.Remove black plastic mulching, outside cuttage case, take Small plastic shed, grown cultures and management detoxification Lilium brownii var viridulum clove seed ball under normal management condition.After cuttage extremely cultivates April, each process scale lining bulb seed ball growing state is in table 1.
After detoxification Lilium brownii var viridulum segment scale safe and efficient cuttage April, seed ball (bulb seed ball) growth indexes is as shown in the table:
。
Claims (4)
1. the safe and efficient scale cuttage technique of detoxification Lilium brownii var viridulum, comprise scale sterilization, scale segment and scale cuttage, it is characterized in that, described method also comprises the low temperature treatment before scale cuttage and segment scale is inorganic without native Light media perlite cuttage vegetative propagation;
Described scale sterilization, adopts chitosan solution to soak;
Described scale segment, is cut into independent 3 sections along vertical long axis, i.e. 1/3 segment scale by skin and middle level scale; Internal layer scale is cut into independent 2 sections along vertical long axis, i.e. 1/2 segment scale.
2. the safe and efficient scale cuttage technique of a kind of detoxification Lilium brownii var viridulum according to claim 1; it is characterized in that; low temperature treatment before described scale cuttage; the 3 years raw cultivation commodity kind balls in detoxification Lilium brownii var viridulum plantlet in vitro land for growing field crops will be gathered in the crops August; select disease-free, reach 35 g ~ 50 g detoxification Lilium brownii var viridulum kind balls without wound, quality; kind ball with a small amount of earth tans by the sun 2 days, puts into 5 DEG C of Storage in cold banks afterwards; 2 DEG C of low-temperature cold store process 2 days are needed before cuttage.
3. the safe and efficient scale cuttage technique of a kind of detoxification Lilium brownii var viridulum according to claim 1, it is characterized in that, described scale is disinfected, and disease-freely waits detoxification Lilium brownii var viridulum kind ball after getting refrigeration without rotting, peel scale gently from outside to inside, put into 100 ppm shitosans and soak 3 hours.
4. the safe and efficient scale cuttage technique of a kind of detoxification Lilium brownii var viridulum according to claim 1, it is characterized in that, described inorganic without native Light media perlite cuttage vegetative propagation, before the cuttage of segment scale, freshly inorganicly shift to an earlier date 3 days without native Light media perlite and tan by the sun, inorganic loading without native Light media perlite will be tanned by the sun and treat cuttage bubble chamber, and appropriate compacting pearlite interstitial substance, and spray stromal surface with 50 ppm shitosans; Segment scale without the need to Plant hormone treatment, by the cuttage down of segment scale biology lower end in inorganic without native Light media pearlite interstitial substance, the cuttage degree of depth 3 cm, cuttage seeding row spacing 5 cm × 5 cm; Cuttage case surrounding leaves pore.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510453975.4A CN104956909B (en) | 2015-07-30 | 2015-07-30 | A kind of safe and efficient scale cuttage technology of detoxification Lilium longiflorum |
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| CN201510453975.4A CN104956909B (en) | 2015-07-30 | 2015-07-30 | A kind of safe and efficient scale cuttage technology of detoxification Lilium longiflorum |
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| Publication Number | Publication Date |
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| CN104956909A true CN104956909A (en) | 2015-10-07 |
| CN104956909B CN104956909B (en) | 2017-03-29 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325272A (en) * | 2015-10-25 | 2016-02-17 | 湖北京穗农林科技发展有限公司 | Lagerstroemia indica cutting breeding method |
| CN106171878A (en) * | 2016-06-30 | 2016-12-07 | 贵州省普安县普白珍稀资源开发有限公司 | A kind of method of Lilies breeding |
| CN106688657A (en) * | 2017-01-05 | 2017-05-24 | 江西省科学院生物资源研究所 | Method of breeding seedball with longya lily scalesunder LED illuminant |
| CN107466628A (en) * | 2017-08-02 | 2017-12-15 | 广西壮族自治区林业科学研究院 | A kind of method of the efficient cutting propagation of giant lily scale |
| CN110959493A (en) * | 2019-11-13 | 2020-04-07 | 湖南工业大学 | Method for preventing lily from degeneration |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1033078A (en) * | 1996-05-22 | 1998-02-10 | Ishikawajima Harima Heavy Ind Co Ltd | Production of bulb of liliaceae plant |
| CN103141381A (en) * | 2013-02-24 | 2013-06-12 | 周世永 | Tissue culture method for oriental lily |
| CN103563611A (en) * | 2013-07-24 | 2014-02-12 | 王运海 | Planting method for lilies |
| CN103907522A (en) * | 2014-04-04 | 2014-07-09 | 天津农学院 | Organic soilless culture medium formula and culture method for edible lily |
| CN104115637A (en) * | 2014-06-25 | 2014-10-29 | 湖南省宝庆农产品进出口有限公司 | Dried lily bulb cultivation method |
| CN104663190A (en) * | 2013-12-03 | 2015-06-03 | 铜仁学院 | Wild lily bulb scale propagation method |
-
2015
- 2015-07-30 CN CN201510453975.4A patent/CN104956909B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1033078A (en) * | 1996-05-22 | 1998-02-10 | Ishikawajima Harima Heavy Ind Co Ltd | Production of bulb of liliaceae plant |
| CN103141381A (en) * | 2013-02-24 | 2013-06-12 | 周世永 | Tissue culture method for oriental lily |
| CN103563611A (en) * | 2013-07-24 | 2014-02-12 | 王运海 | Planting method for lilies |
| CN104663190A (en) * | 2013-12-03 | 2015-06-03 | 铜仁学院 | Wild lily bulb scale propagation method |
| CN103907522A (en) * | 2014-04-04 | 2014-07-09 | 天津农学院 | Organic soilless culture medium formula and culture method for edible lily |
| CN104115637A (en) * | 2014-06-25 | 2014-10-29 | 湖南省宝庆农产品进出口有限公司 | Dried lily bulb cultivation method |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325272A (en) * | 2015-10-25 | 2016-02-17 | 湖北京穗农林科技发展有限公司 | Lagerstroemia indica cutting breeding method |
| CN106171878A (en) * | 2016-06-30 | 2016-12-07 | 贵州省普安县普白珍稀资源开发有限公司 | A kind of method of Lilies breeding |
| CN106688657A (en) * | 2017-01-05 | 2017-05-24 | 江西省科学院生物资源研究所 | Method of breeding seedball with longya lily scalesunder LED illuminant |
| CN107466628A (en) * | 2017-08-02 | 2017-12-15 | 广西壮族自治区林业科学研究院 | A kind of method of the efficient cutting propagation of giant lily scale |
| CN110959493A (en) * | 2019-11-13 | 2020-04-07 | 湖南工业大学 | Method for preventing lily from degeneration |
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| CN104956909B (en) | 2017-03-29 |
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