CN107466628A - A kind of method of the efficient cutting propagation of giant lily scale - Google Patents

A kind of method of the efficient cutting propagation of giant lily scale Download PDF

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Publication number
CN107466628A
CN107466628A CN201710649620.1A CN201710649620A CN107466628A CN 107466628 A CN107466628 A CN 107466628A CN 201710649620 A CN201710649620 A CN 201710649620A CN 107466628 A CN107466628 A CN 107466628A
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scale
bulb
cutting
preculture
giant lily
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肖玉菲
刘海龙
陈博雯
覃子海
刘雄盛
张烨
张晓宁
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Guangxi Zhuang Autonomous Region Forestry Research Institute
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Guangxi Zhuang Autonomous Region Forestry Research Institute
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
    • A01G13/0243—Protective shelters for young plants, e.g. tubular sleeves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Environmental Sciences (AREA)
  • Ecology (AREA)
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  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Soil Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cultivation Of Plants (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

A kind of method of the efficient cutting propagation of giant lily scale, comprising bulb preculture, scale sterilization, scale processing, cutting medium preparation, scale cuttage and insert rear management process.The present invention after scale sterilization from cutting propagation is carried out, and method is simple and easy, and condition is easily controlled, and while maternal character is kept, is avoided bulb and is degenerated and virus accumulation；20 DEG C of precultures are carried out to bulb before scale cuttage, reduce the germ entrained by bulb itself, and cuttage is not constrained by season；It is carried out longitudinally cutting during scale processing, greatly improve breeding coefficient, breeding coefficient can increase to more than 4.0 with common scale cuttage compared with, and compared to clove breeding, its breeding coefficient adds decades of times；After scale cuttage 3d into matrix the mg/L of sprinkle 250 ABT 2#Solution, scale Rapid Rooting can be promoted, 10d or so is taken root, and rooting rate is up to more than 98%, 30d or so and forms bulb, and a large amount of aseptic giant lily bulbs of health can be obtained in the short time, are adapted to large-scale breeding giant lily, market application foreground is wide.

Description

A kind of method of the efficient cutting propagation of giant lily scale
Technical field
The invention belongs to plant cuttage vegetative propagation technique field, and in particular to a kind of efficient cutting propagation of giant lily scale Method.
Background technology
Giant lily (Cardiocrinum giganteum), also known as lobus cardiacus lily, water lily, it is Liliaceae (Liliaceae ) the extremely cold-resistant plant of Cardiocrinum (Cardiocrinum), because plant is very large grand, beautiful fragrance, it is substantially distinguished from lily Platymiscium and gain the name, its plant is sturdy tall and straight, the pure white fragrance of flower, there is the good reputation of lily prince, widely distributed in China.In recent years Come, by introduction and acclimatization and artificial cultivation, giant lily turns into excellent ornamental plant.In addition, its bulb is rich in starch and 10 Multiple nutritional components, can be edible.Meanwhile giant lily also has higher medical value, not only can with tonifying middle-Jiao and Qi, clear away heart-fire Calm the nerves, wide chest profit gas, moisten the lung and relieve the cough, detoxify, available for hemoptysis of pulmonary tuberculosis, nasosinusitis, tympanitis, children's high fever, stomachache and anti- The illnesss such as stomach, vomiting, and modern medicine research finds that giant lily also has antitumaous effect.Therefore, giant lily is that one kind has concurrently The plant of ornamental value, edibility and medical value, application development prospect are very wide.
The modes of reproduction generally use generative propagation of giant lily and bulb separation breeding, during generative propagation, giant lily seed is usual It could be sprouted by two cold stages, growth of seedling is extremely slow, and bulb separation breeding can cause bulb to diminish and degenerate, even Virus accumulation, should not frequently divide cultivation, and low reproduction rate, be constrained by seasonal conditions, both the above modes of reproduction has a strong impact on big by hundred The yield and ornamental value of conjunction, it is not suitable for the needs of biological control, far from meeting the needs of market.Using scale cuttage Breeding, can obtain a large amount of bulbs in a short time, and can keep maternal merit.Liliaceous plant scale skewer at present Insert technical research majority concentrate on lily, but generally occur in existing lily cuttage technique scale rot, Root survival is low, breeding coefficient is not high, had a great influence etc. problems by seasonal factor, it is impossible to reaches preferable effect, no Suitably promote the use of a large area, and there is not been reported for the technical research of giant lily scale cuttage.
The content of the invention
The purpose of the present invention is overcome the deficiencies in the prior art, there is provided a kind of efficiently convenient, simple to operate, rooting rate stabilization A kind of method of efficient cutting propagation of giant lily scale of application easy to spread, it is quick to obtain substantial amounts of excellent giant lily bulb, For the substantial amounts of nursery stock demand of in the market.
To achieve these goals, the technical solution adopted by the present invention is：
Characterized in that, comprising bulb preculture, scale sterilization, scale processing, cutting medium prepare, scale cuttage and insert after manage Reason, key step are as follows：
（1）Bulb preculture：In July ~ September, giant lily bulb is gathered, preparation peat soil, which is fitted into plastic crate, is used as pre-culture medium Matter, the preculture matrix is first drenched 2 times with 0.5 % liquor potassic permanganates, then is drenched with clear water, the bulb of collection is embedded to pre- In culture matrix, it is positioned in the air-conditioned room of 20 DEG C of constant temperature and carries out bulb preculture；
（2）Scale sterilizes：The d of preculture >=14 bulb is dug out, flowing water rinses surface soil and debris, removes the epidermis that rots, Scale is successively peeled, as far as possible member-retaining portion plateau, soft gauze gently cleans scale surface, and it is 5 %'s to be put into mass fraction 10 min are soaked in washing powder solution, cut scale top nigrescence part, flowing water rinses 1 h, is put into 75 % alcoholic solutions and soaks 1 min is steeped, pure water rinses 5 ~ 6 times；
（3）Scale processing：Scale after sterilization is subjected to scale cutting, otch is sopped up with the blotting paper after autoclaving after cutting Leachate, then the biology bottom for cutting scale is soaked in the h of 1 h in the nutrient solution after autoclaving ~ 2, then be transferred to and take root The h of 1 h ~ 4 is soaked in agent, concave surface is down；
（4）Cutting medium prepares：Well mixed peat soil and perlite are first admixed as cutting medium, the cutting medium 50% 500 times of liquid of carbendazol wettable powder carry out disinfection, and are fitted into plastic crate, and one layer of modeling is first padded in the bottom of the plastic crate Expect cloth, the corner of plastic cloth is sling slightly, keeps the water content of cutting medium in 40% ~ 60 %；
（5）Scale cuttage：Scale after processing is inserted perpendicularly into matrix according to biology bottom direction directed downwardly, insertion depth For the 3/4 of scale, the spacing of scale and scale is >=5 cm, then uniformly spills the root-growing agent for handling scale around scale；
（6）Managed after inserting：Straw of the thickness for the cm of 1.5cm~2.5 is covered after inserting above scale, is positioned over 24 DEG C of sky In transferring house, the indoor % of holding humidity 60%~70, cutting medium keeps certain moisture, and 250 are sprayed into cutting medium after 3 d Mg/L ABT 2#Solution.
Above step（1）The giant lily bulb is >=2 years non-hibernating eggs balls, kind bulb diameter >=10 cm.
Above step（1）Depth in the bulb embedment preculture matrix is the 5/6 of bulb, is stayed between bulb and bulb There is >=10 cm gap.
Above step（1）The bulb preculture must be every 3 d water drenchings once, and it is excessive to be sure not water drenching, in case bulb rots, Kind of ball surfaces and preculture matrix are sprayed with 50 % carbendazol wettable powders, 500 times of liquid once, every 7 d to bulb and in advance Culture matrix carries out disinfection.
Above step（3）The mode of scale cutting is：Blade carries out longitudinal direction from top to bottom perpendicular to plateau direction Cutting, according to scale size, is cut into 2 ~ 4 equal portions, each equal portions retain some plateaus by scale.
Above step（3）The component and content of the nutrient solution be：NH4NO3 400 mg/L；KNO3 280 mg/L； MgSO4·7H2O 370 mg/L CaCl2·2H2O 85 mg/L；CuSO4·5 H2O 0.025 mg/L；FeSO4·7H2O 27.8 mg/L；ZnSO4·7 H2O 2 mg/L； MnSO4·4 H2O 22.3 mg/L；H3BO33 mg/L；Na2MoO4·2 H2O 0.25 mg/L；The mg/L of inositol 100；The mg/L of glycine 2.0；The mg/L of nicotinic acid 0.5；The mg/L of vitamin B1 0.1； The mg/L of vitamin B6 0.5；PH is 5.5 ~ 6.5, and root-growing agent is 500 mg/L ABT 2#Solution.
Above step（4）The raw material proportioning of the cutting medium is：Peat soil：Perlite=5:3.
Above step（6）The cutting medium keeps certain moisture to refer to that cutting medium is loose not into sticking together in black, moulds Without ponding on charging basket bottom plastic cloth.
Above step（1）With（4）The length × width × height of the plastic crate is the cm of 45 cm × 35cm × 25.
Compared with the prior art, the invention has the advantages that：
（1）For the present invention from cutting propagation is carried out after scale sterilization, method is simple and easy, and condition is easily controlled, convenient and practical, While keeping maternal character, avoid bulb and degenerate and virus accumulation, bred compared to clove, its breeding coefficient adds number Ten times, it is adapted to large-scale breeding giant lily, market application foreground is wide.
（2）The present invention carries out 20 DEG C of precultures before scale cuttage to bulb, can not only gradually reduce bulb itself institute The germ of carrying, and bulb can be stored, cuttage process is carried out indoors, avoids germ infringement, and cuttage is not by season Constraint.
（3）The present invention carries out longitudinally cutting when scale is handled to it, and soaks overnutrition liquid and fully absorbs water and seek Rooting treatment is carried out again after supporting material, is avoided after scale is cut because rooting efficiency caused by nutriment deficiency is undesirable, Scale various pieces rooting efficiency is good, can greatly improve breeding coefficient, compared with common scale cuttage, breeding coefficient increase To more than 4.0.
（4）The present invention holds cuttage and culture matrix using plastic crate, good permeability, avoids scale and rots.
（5）The present invention, in scale surface mulching straw, avoids water loss, can dramatically protected after cuttage It is wet, and uniform moisture is impregnated with matrix when being advantageous to water drenching.
（6）After the d of scale cuttage 3 of the present invention into matrix the mg/L of sprinkle 250 ABT 2#Solution, root-growing agent can be strengthened Effect, promote scale Rapid Rooting, 10 d or so take root, rooting rate is fast, and rooting rate is up to 98 more than %, 30 d or so shapes Into bulb, a large amount of aseptic giant lily bulbs of health can be obtained in a short time.
Brief description of the drawings
Fig. 1 is giant lily scale rooting efficiency figure.
Fig. 2 is that giant lily scale regenerates multiple clove design sketch.
Embodiment
The following examples can make those skilled in the art that the present invention be more fully understood, but not limit in any way The present invention.It is to be understood by those skilled in the art that various changes can be made to it in the form and details, and Without departing from claims of the present invention limited range.
Embodiment 1：
（1）Bulb preculture：In September in 2016 8 days, diameter >=10cm 2 years raw giant lily bulbs are gathered, prepares peat soil and fills Enter in plastic crate of the length × width × height for the cm of the cm of 45 cm × 35 × 25 and be used as precultivation medium matter, the preculture matrix First drenched culture matrix 2 times with 0.5 % liquor potassic permanganates, then drenched with clear water, the bulb of collection is embedded to preculture matrix In, the depth in bulb embedment preculture matrix is the 5/6 of bulb, and 10 cm gap is left between bulb and bulb, is positioned over perseverance Bulb preculture is carried out in 20 DEG C of air-conditioned room of temperature, every 3 d water drenchings once, it is excessive to be sure not water drenching, in case bulb rots, often Kind of ball surfaces and preculture matrix are sprayed with 50 % carbendazol wettable powders, 500 times of liquid once, every 7 d to bulb and pre- training Foster matrix carries out disinfection.
（2）Scale sterilizes：Bulb after the d of preculture 14 is dug out, flowing water rinses surface soil and debris, removes and rots Epidermis, scale is successively peeled, as far as possible member-retaining portion plateau, soft gauze gently cleans scale surface, is put into mass fraction as 5 10 min are soaked in % washing powder solution, cut scale top nigrescence part, flowing water rinses 1 h, is put into 75 % alcoholic solutions 1 min is soaked, pure water rinses 5 times.
（3）Scale processing：Scale after sterilization is subjected to scale cutting, scale cutting mode is blade perpendicular to plateau Direction carries out longitudinally cutting from top to bottom, the leachate of otch is sopped up after cutting with the blotting paper after autoclaving, according to scale Size, scale is cut into 2 ~ 4 equal portions, each equal portions retain some plateaus, then soak the biology bottom for cutting scale 1 h in nutrient solution is steeped, then is transferred to 500 mg/L ABT 2#4 h are soaked in solution, concave surface is down.
（4）Cutting medium prepares：By well mixed peat soil：Perlite（5:3）, admix 50% carbendazim wettable 500 times of liquid of pulvis carry out disinfection, and are fitted into plastic crate of the length × width × height for the cm of the cm of 45 cm × 35 × 25, the plastic crate Bottom first pad one layer of plastic cloth, the corner of plastic cloth is sling slightly, keeps the water content of cutting medium in the % of 35 % ~ 50.
（5）Scale cuttage：Scale after processing is inserted perpendicularly into matrix according to biology bottom direction directed downwardly, inserted Depth is the 3/4 of scale, and the spacing of scale and scale is 5 cm, is then uniformly spilt the root-growing agent for handling scale in scale week Enclose.
（6）Managed after inserting：The straw that a thickness is 2.0cm is covered after inserting above scale, is positioned over 24 DEG C of air-conditioned room Interior, the % of the indoor % of holding humidity 60 ~ 65, cutting medium keeps certain moisture（Substrate soil is loose not into sticking together in black, plastic crate Without ponding on bottom plastic cloth）, after 3 d into cutting medium the mg/L of sprinkle 250 ABT 2#Solution.Scale is taken root after 15 d, Rooting rate 98.2%, bulb is formed after 35d.
Embodiment 2：
（1）Bulb preculture：In September in 2016 8 days, diameter >=13cm 2 years raw giant lily bulbs are gathered, prepares peat soil and fills Enter in plastic crate of the length × width × height for the cm of the cm of 45 cm × 35 × 25 and be used as precultivation medium matter, the preculture matrix First drenched culture matrix 2 times with 0.5 % liquor potassic permanganates, then drenched with clear water, the bulb of collection is embedded to preculture matrix In, the depth in bulb embedment preculture matrix is the 5/6 of bulb, and 15 cm gap is left between bulb and bulb, is positioned over perseverance Bulb preculture is carried out in 20 DEG C of air-conditioned room of temperature, every 3 d water drenchings once, it is excessive to be sure not water drenching, in case bulb rots, often Kind of ball surfaces and preculture matrix are sprayed with 50 % carbendazol wettable powders, 500 times of liquid once, every 7 d to bulb and pre- training Foster matrix carries out disinfection.
（2）Scale sterilizes：Bulb after the d of preculture 20 is dug out, flowing water rinses surface soil and debris, removes and rots Epidermis, scale is successively peeled, as far as possible member-retaining portion plateau, soft gauze gently cleans scale surface, is put into mass fraction as 5 10 min are soaked in % washing powder solution, cut scale top nigrescence part, flowing water rinses 1 h, is put into 75 % alcoholic solutions 1 min is soaked, pure water rinses 5 times.
（3）Scale processing：Scale after sterilization is subjected to scale cutting, scale cutting mode is blade perpendicular to plateau Direction carries out longitudinally cutting from top to bottom, the leachate of otch is sopped up after cutting with the blotting paper after autoclaving, according to scale Size, scale is cut into 2 ~ 4 equal portions, each equal portions retain some plateaus, then soak the biology bottom for cutting scale 2 h in nutrient solution is steeped, then is transferred to 500 mg/L ABT 2#3h is soaked in solution, concave surface is down.
（4）Cutting medium prepares：By well mixed peat soil：Perlite（5:3）, admix 50% carbendazim wettable 500 times of liquid of pulvis carry out disinfection, and are fitted into plastic crate of the length × width × height for the cm of the cm of 45 cm × 35 × 25, the plastic crate Bottom first pad one layer of plastic cloth, the corner of plastic cloth is sling slightly, keeps the water content of cutting medium in the % of 40 % ~ 50.
（5）Scale cuttage：Scale after processing is inserted perpendicularly into matrix according to biology bottom direction directed downwardly, inserted Depth is the 3/4 of scale, and the spacing of scale and scale is 6 cm, is then uniformly spilt the root-growing agent for handling scale in scale week Enclose.
（6）Managed after inserting：The straw that a thickness is 2.5 cm is covered after inserting above scale, is positioned over 24 DEG C of air-conditioned room Interior, the % of the indoor % of holding humidity 65 ~ 70, cutting medium keeps certain moisture（Substrate soil is loose not into sticking together in black, plastic crate Without ponding on bottom plastic cloth）, after 3 d into cutting medium the mg/L of sprinkle 250 ABT 2#Solution.Scale is taken root after 12d, Rooting rate 99%, bulb is formed after 32d.
Embodiment 3：
（1）Bulb preculture：In August in 2016 16 days, diameter >=12cm 2 years raw giant lily bulbs, preparation peat soil are gathered It is fitted into plastic crate of the length × width × height for the cm of the cm of 45 cm × 35 × 25 and is used as precultivation medium matter, the pre-culture medium Matter is first drenched culture matrix 2 times with 0.5 % liquor potassic permanganates, then is drenched with clear water, and the bulb of collection is embedded into pre-culture medium In matter, the depth in bulb embedment preculture matrix is the 5/6 of bulb, and 15 cm gap is left between bulb and bulb, is positioned over Bulb preculture is carried out in the air-conditioned room that 20 DEG C of constant temperature, every 3 d water drenchings once, it is excessive to be sure not water drenching, in case bulb rots, Kind of ball surfaces and preculture matrix are sprayed with 50 % carbendazol wettable powders, 500 times of liquid once, every 7 d to bulb and in advance Culture matrix carries out disinfection.
（2）Scale sterilizes：Bulb after the d of preculture 20 is dug out, flowing water rinses surface soil and debris, removes and rots Epidermis, scale is successively peeled, as far as possible member-retaining portion plateau, soft gauze gently cleans scale surface, is put into mass fraction as 5 10 min are soaked in % washing powder solution, cut scale top nigrescence part, flowing water rinses 1 h, is put into 75 % alcoholic solutions 1 min is soaked, pure water rinses 5 times.
（3）Scale processing：Scale after sterilization is subjected to scale cutting, scale cutting mode is blade perpendicular to plateau Direction carries out longitudinally cutting from top to bottom, the leachate of otch is sopped up after cutting with the blotting paper after autoclaving, according to scale Size, scale is cut into 2 ~ 4 equal portions, each equal portions retain some plateaus, then soak the biology bottom for cutting scale 2 h in nutrient solution is steeped, then is transferred to 500 mg/L ABT 2#3 h are soaked in solution, concave surface is down.
（4）Cutting medium prepares：By well mixed peat soil：Perlite（5:3）, admix 50% carbendazim wettable 500 times of liquid of pulvis carry out disinfection, and are fitted into plastic crate of the length × width × height for the cm of the cm of 45 cm × 35 × 25, the plastic crate Bottom first pad one layer of plastic cloth, the corner of plastic cloth is sling slightly, keeps the water content of cutting medium in the % of 40 % ~ 60.
（5）Scale cuttage：Scale after processing is inserted perpendicularly into matrix according to biology bottom direction directed downwardly, inserted Depth is the 3/4 of scale, and the spacing of scale and scale is 6 cm, is then uniformly spilt the root-growing agent for handling scale in scale week Enclose.
（6）Managed after inserting：The straw that a thickness is 2.0 cm is covered after inserting above scale, is positioned over 24 DEG C of air-conditioned room Interior, the % of the indoor % of holding humidity 65 ~ 70, cutting medium keeps certain moisture（Substrate soil is loose not into sticking together in black, plastic crate Without ponding on bottom plastic cloth）, after 3 d into cutting medium the mg/L of sprinkle 250 ABT 2#Solution.Scale is taken root after 12d, Rooting rate 99.6%, bulb is formed after 31d.
Embodiment 4：
（1）Bulb preculture：On July 20th, 2016, diameter >=10cm 2 years raw giant lily bulbs are gathered, prepare peat soil It is fitted into plastic crate of the length × width × height for the cm of the cm of 45 cm × 35 × 25 and is used as precultivation medium matter, the pre-culture medium Matter is first drenched culture matrix 2 times with 0.5 % liquor potassic permanganates, then is drenched with clear water, and the bulb of collection is embedded into pre-culture medium In matter, the depth in bulb embedment preculture matrix is the 5/6 of bulb, and 15 cm gap is left between bulb and bulb, is positioned over Bulb preculture is carried out in the air-conditioned room that 20 DEG C of constant temperature, every 3 d water drenchings once, it is excessive to be sure not water drenching, in case bulb rots, Kind of ball surfaces and preculture matrix are sprayed with 50 % carbendazol wettable powders, 500 times of liquid once, every 7 d to bulb and in advance Culture matrix carries out disinfection.
（2）Scale sterilizes：Bulb after the d of preculture 20 is dug out, flowing water rinses surface soil and debris, removes and rots Epidermis, scale is successively peeled, as far as possible member-retaining portion plateau, soft gauze gently cleans scale surface, is put into mass fraction as 5 10 min are soaked in % washing powder solution, cut scale top nigrescence part, flowing water rinses 1 h, is put into 75 % alcoholic solutions 1 min is soaked, pure water rinses 5 times.
（3）Scale processing：Scale after sterilization is subjected to scale cutting, scale cutting mode is blade perpendicular to plateau Direction carries out longitudinally cutting from top to bottom, the leachate of otch is sopped up after cutting with the blotting paper after autoclaving, according to scale Size, scale is cut into 2 ~ 4 equal portions, each equal portions retain some plateaus, then soak the biology bottom for cutting scale 1.5 h in nutrient solution is steeped, then is transferred to 500 mg/L ABT 2#2 h are soaked in solution, concave surface is down.
（4）Cutting medium prepares：By well mixed peat soil：Perlite（5:3）, admix 50% carbendazim wettable 500 times of liquid of pulvis carry out disinfection, and are fitted into plastic crate of the length × width × height for the cm of the cm of 45 cm × 35 × 25, the plastic crate Bottom first pad one layer of plastic cloth, the corner of plastic cloth is sling slightly, keeps the water content of cutting medium in the % of 40 % ~ 60.
（5）Scale cuttage：Scale after processing is inserted perpendicularly into matrix according to biology bottom direction directed downwardly, inserted Depth is the 3/4 of scale, and the spacing of scale and scale is 6 cm, is then uniformly spilt the root-growing agent for handling scale in scale week Enclose.
（6）Managed after inserting：The straw that a thickness is 1.5 cm is covered after inserting above scale, is positioned over 24 DEG C of air-conditioned room Interior, the indoor % of holding humidity 70, cutting medium keeps certain moisture（Substrate soil is loose not into sticking together in black, plastic crate bottom Without ponding on plastic cloth）, after 3 d into cutting medium the mg/L of sprinkle 250 ABT 2#Solution.Scale is taken root after 12d, is taken root Rate 98.5%, bulb is formed after 34d.
Each example step above（3）The component and content of the nutrient solution be：NH4NO3 400 mg/L；KNO3 280 mg/L； MgSO4·7H2O 370 mg/L CaCl2·2H2O 85 mg/L；CuSO4·5 H2O 0.025 mg/L；FeSO4·7H2O 27.8 mg/L；ZnSO4·7 H2O 2 mg/L； MnSO4·4 H2O 22.3 mg/L；H3BO33 mg/L；Na2MoO4·2 H2O 0.25 mg/L；The mg/L of inositol 100；The mg/L of glycine 2.0；The mg/L of nicotinic acid 0.5；The mg/L of vitamin B1 0.1； The mg/L of vitamin B6 0.5；PH is 5.5 ~ 6.5.

Claims (9)

  1. A kind of 1. method of the efficient cutting propagation of giant lily scale, it is characterised in that include bulb preculture, scale sterilization, squama Piece processing, cutting medium prepare, scale cuttage and insert after manage, key step is as follows：
    （1）Bulb preculture：In July ~ September, giant lily bulb is gathered, preparation peat soil, which is fitted into plastic crate, is used as pre-culture medium Matter, the preculture matrix is first drenched 2 times with 0.5 % liquor potassic permanganates, then is drenched with clear water, the bulb of collection is embedded to pre- In culture matrix, it is positioned in the air-conditioned room of 20 DEG C of constant temperature and carries out bulb preculture；
    （2）Scale sterilizes：The d of preculture >=14 bulb is dug out, flowing water rinses surface soil and debris, removes the epidermis that rots, Scale is successively peeled, as far as possible member-retaining portion plateau, soft gauze gently cleans scale surface, and it is 5 %'s to be put into mass fraction 10 min are soaked in washing powder solution, cut scale top nigrescence part, flowing water rinses 1 h, is put into 75 % alcoholic solutions and soaks 1 min is steeped, pure water rinses 5 ~ 6 times；
    （3）Scale processing：Scale after sterilization is subjected to scale cutting, otch is sopped up with the blotting paper after autoclaving after cutting Leachate, then the biology bottom for cutting scale is soaked in the h of 1 h in the nutrient solution after autoclaving ~ 2, then be transferred to and take root The h of 1 h ~ 4 is soaked in agent, concave surface is down；
    （4）Cutting medium prepares：Well mixed peat soil and perlite are first admixed as cutting medium, the cutting medium 50% 500 times of liquid of carbendazol wettable powder carry out disinfection, and are fitted into plastic crate, and one layer of modeling is first padded in the bottom of the plastic crate Expect cloth, the corner of plastic cloth is sling slightly, keeps the water content of cutting medium in 40% ~ 60 %；
    （5）Scale cuttage：Scale after processing is inserted perpendicularly into matrix according to biology bottom direction directed downwardly, insertion depth For the 3/4 of scale, the spacing of scale and scale is >=5 cm, then uniformly spills the root-growing agent for handling scale around scale；
    （6）Managed after inserting：Straw of the thickness for the cm of 1.5cm~2.5 is covered after inserting above scale, is positioned over 24 DEG C of sky In transferring house, the indoor % of holding humidity 60%~70, cutting medium keeps certain moisture, and 250 are sprayed into cutting medium after 3 d Mg/L ABT 2#Solution.
  2. 2. the method for the efficient cutting propagation of giant lily scale according to claim 1, it is characterised in that step（1）It is described Giant lily bulb is >=2 years non-hibernating eggs balls, kind bulb diameter >=10 cm.
  3. 3. the method for the efficient cutting propagation of giant lily scale according to claim 1, it is characterised in that step（1）It is described Depth in bulb embedment preculture matrix is the 5/6 of bulb, and >=10 cm gap is left between bulb and bulb.
  4. 4. the method for the efficient cutting propagation of giant lily scale according to claim 1, it is characterised in that step（1）It is described Bulb preculture must be every 3 d water drenchings once, and it is excessive to be sure not water drenching, in case bulb rots, can with 50 % carbendazim every 7 d 500 times of liquid spray kind ball surfaces of WP and preculture matrix once, carry out disinfection to bulb and preculture matrix.
  5. 5. the method for the efficient cutting propagation of giant lily scale according to claim 1, it is characterised in that step（3）It is described Scale cutting mode be：Blade carried out from top to bottom perpendicular to plateau direction it is longitudinally cutting, according to scale size, by scale 2 ~ 4 equal portions are cut into, each equal portions retain some plateaus.
  6. 6. the method for the efficient cutting propagation of giant lily scale according to claim 1, it is characterised in that step（3）It is described The component and content of nutrient solution be：NH4NO3 400 mg/L；KNO3 280 mg/L；MgSO4·7H2O 370 mg/L CaCl2· 2H2O 85 mg/L；CuSO4·5 H2O 0.025 mg/L；FeSO4·7H2O 27.8 mg/L；ZnSO4·7 H2O 2 mg/L； MnSO4·4 H2O 22.3 mg/L；H3BO33 mg/L；Na2MoO4·2 H2O 0.25 mg/L；The mg/L of inositol 100；Sweet ammonia 2.0 mg/L of acid；The mg/L of nicotinic acid 0.5；The mg/L of vitamin B1 0.1；The mg/L of vitamin B6 0.5；PH is 5.5 ~ 6.5, raw Root agent is 500 mg/L ABT 2#Solution.
  7. 7. the method for the efficient cutting propagation of giant lily scale according to claim 1, it is characterised in that step（4）It is described The raw material proportioning of cutting medium is：Peat soil：Perlite=5:3.
  8. 8. the method for the efficient cutting propagation of giant lily scale according to claim 1, it is characterised in that step（6）It is described Cutting medium keeps certain moisture to refer to that cutting medium is loose not into sticking together in black, without ponding on plastic crate bottom plastic cloth.
  9. 9. the method for the efficient cutting propagation of giant lily scale according to claim 1, it is characterised in that step（1）With （4）The length × width × height of the plastic crate is the cm of 45 cm × 35cm × 25.
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Cited By (9)

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Publication number Priority date Publication date Assignee Title
CN107889630A (en) * 2017-12-21 2018-04-10 安庆市枞阳县阳和苗圃 A kind of scale cuttage method for culturing seedlings of lily
CN110537427A (en) * 2019-10-11 2019-12-06 阿坝师范学院 lilium regale scale cuttage method
CN111771657A (en) * 2020-07-13 2020-10-16 东北师范大学 Cultivation method of lilium floribundum
CN111903519A (en) * 2020-07-31 2020-11-10 广西壮族自治区林业科学研究院 Method for inducing lilium davidii to root after dormancy releasing
CN112088740A (en) * 2020-10-15 2020-12-18 山东庆丰农业种植科技开发有限公司 Edible lily root cutting potting method
CN113455391A (en) * 2021-06-16 2021-10-01 广西壮族自治区林业科学研究院 Propagation method for tissue culture propagation of large lily by taking seeds as explants
CN113455391B (en) * 2021-06-16 2022-06-10 广西壮族自治区林业科学研究院 Propagation method for tissue culture propagation of large lily by taking seeds as explants
CN114375790A (en) * 2021-12-24 2022-04-22 上海上房园林植物研究所有限公司 Cutting propagation method of mediterranean sciaenopsis jujuba
CN115735584A (en) * 2022-10-28 2023-03-07 华中农业大学 Lily scale cuttage method capable of improving cuttage propagation coefficient and bulb size

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