CN108077022A - For the matrix of blueberry hardening and blueberry hardening off method - Google Patents
For the matrix of blueberry hardening and blueberry hardening off method Download PDFInfo
- Publication number
- CN108077022A CN108077022A CN201711361352.XA CN201711361352A CN108077022A CN 108077022 A CN108077022 A CN 108077022A CN 201711361352 A CN201711361352 A CN 201711361352A CN 108077022 A CN108077022 A CN 108077022A
- Authority
- CN
- China
- Prior art keywords
- hardening
- matrix
- blueberry
- parts
- mass parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011159 matrix material Substances 0.000 title claims abstract description 106
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 72
- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 72
- 235000021014 blueberries Nutrition 0.000 title claims abstract description 72
- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 64
- 238000000034 method Methods 0.000 title claims abstract description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 89
- 239000002904 solvent Substances 0.000 claims abstract description 35
- 239000002689 soil Substances 0.000 claims abstract description 21
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 19
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 239000002131 composite material Substances 0.000 claims abstract description 12
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000005864 Sulphur Substances 0.000 claims abstract description 10
- 239000002361 compost Substances 0.000 claims abstract description 10
- 240000007817 Olea europaea Species 0.000 claims abstract description 8
- 239000000243 solution Substances 0.000 claims description 25
- 239000007921 spray Substances 0.000 claims description 21
- 229920003023 plastic Polymers 0.000 claims description 15
- 239000004033 plastic Substances 0.000 claims description 15
- 239000004698 Polyethylene Substances 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 14
- -1 polyethylene Polymers 0.000 claims description 14
- 229920000573 polyethylene Polymers 0.000 claims description 14
- 230000000855 fungicidal effect Effects 0.000 claims description 12
- 239000000417 fungicide Substances 0.000 claims description 11
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 10
- 240000002234 Allium sativum Species 0.000 claims description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 235000004611 garlic Nutrition 0.000 claims description 9
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 claims description 8
- 235000003097 Artemisia absinthium Nutrition 0.000 claims description 8
- 240000001851 Artemisia dracunculus Species 0.000 claims description 8
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 claims description 8
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims description 8
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 8
- 235000011613 Pinus brutia Nutrition 0.000 claims description 8
- 241000018646 Pinus brutia Species 0.000 claims description 8
- 239000011504 laterite Substances 0.000 claims description 8
- 229910001710 laterite Inorganic materials 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 8
- 239000005802 Mancozeb Substances 0.000 claims description 7
- 240000008013 Phytolacca acinosa Species 0.000 claims description 7
- 235000009076 Phytolacca acinosa Nutrition 0.000 claims description 7
- 239000001138 artemisia absinthium Substances 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 7
- 239000012895 dilution Substances 0.000 claims description 7
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 7
- 244000099147 Ananas comosus Species 0.000 claims description 6
- 235000007119 Ananas comosus Nutrition 0.000 claims description 6
- 244000245109 Lindera glauca Species 0.000 claims description 6
- 235000001577 Lindera glauca Nutrition 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 235000019362 perlite Nutrition 0.000 claims description 6
- 239000010451 perlite Substances 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 239000002023 wood Substances 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 229930191978 Gibberellin Natural products 0.000 claims description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 5
- 239000005030 aluminium foil Substances 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 5
- 239000003337 fertilizer Substances 0.000 claims description 5
- 239000001530 fumaric acid Substances 0.000 claims description 5
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 5
- 239000003448 gibberellin Substances 0.000 claims description 5
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 5
- 239000011701 zinc Substances 0.000 claims description 5
- 229910052725 zinc Inorganic materials 0.000 claims description 5
- 244000153158 Ammi visnaga Species 0.000 claims description 4
- 235000010585 Ammi visnaga Nutrition 0.000 claims description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 4
- 239000011777 magnesium Substances 0.000 claims description 4
- 229910052749 magnesium Inorganic materials 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 241001474374 Blennius Species 0.000 claims description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 3
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 3
- 229910052796 boron Inorganic materials 0.000 claims description 3
- YREQHYQNNWYQCJ-UHFFFAOYSA-N etofenprox Chemical compound C1=CC(OCC)=CC=C1C(C)(C)COCC1=CC=CC(OC=2C=CC=CC=2)=C1 YREQHYQNNWYQCJ-UHFFFAOYSA-N 0.000 claims description 3
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 3
- 235000011151 potassium sulphates Nutrition 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 150000003217 pyrazoles Chemical class 0.000 claims description 3
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- 150000002475 indoles Chemical class 0.000 claims 1
- 229940072033 potash Drugs 0.000 claims 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims 1
- 235000015320 potassium carbonate Nutrition 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 24
- 238000009264 composting Methods 0.000 abstract description 9
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 239000010408 film Substances 0.000 description 17
- 230000008569 process Effects 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 8
- 229920003266 Leaf® Polymers 0.000 description 8
- 244000077233 Vaccinium uliginosum Species 0.000 description 8
- 239000004615 ingredient Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 230000000844 anti-bacterial effect Effects 0.000 description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 241000193830 Bacillus <bacterium> Species 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 241001420287 Strobilanthes maculata Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 230000000840 anti-viral effect Effects 0.000 description 4
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- 239000005794 Hymexazol Substances 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 241000194017 Streptococcus Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- KGVPNLBXJKTABS-UHFFFAOYSA-N hymexazol Chemical compound CC1=CC(O)=NO1 KGVPNLBXJKTABS-UHFFFAOYSA-N 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000193738 Bacillus anthracis Species 0.000 description 2
- 241001478240 Coccus Species 0.000 description 2
- 241000186227 Corynebacterium diphtheriae Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- 239000005869 Pyraclostrobin Substances 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 208000037386 Typhoid Diseases 0.000 description 2
- 240000008424 Vaccinium ashei Species 0.000 description 2
- 235000013468 Vaccinium ashei Nutrition 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 229940065181 bacillus anthracis Drugs 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000002070 germicidal effect Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000011169 microbiological contamination Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 201000008297 typhoid fever Diseases 0.000 description 2
- STGNLGBPLOVYMA-MAZDBSFSSA-N (E)-but-2-enedioic acid Chemical compound OC(=O)\C=C\C(O)=O.OC(=O)\C=C\C(O)=O STGNLGBPLOVYMA-MAZDBSFSSA-N 0.000 description 1
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 1
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- VGAJNILINWUWOP-UHFFFAOYSA-N Eudesmane Natural products COC(=O)C(=C)C1C(O)C2C(=O)CCC(O)C2(C)CC1OC(=O)C(=C)CO VGAJNILINWUWOP-UHFFFAOYSA-N 0.000 description 1
- 239000001263 FEMA 3042 Substances 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241000606768 Haemophilus influenzae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 1
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 240000003889 Piper guineense Species 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- YZCKVEUIGOORGS-IGMARMGPSA-N Protium Chemical compound [1H] YZCKVEUIGOORGS-IGMARMGPSA-N 0.000 description 1
- 241000607762 Shigella flexneri Species 0.000 description 1
- 241000607760 Shigella sonnei Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 241000736767 Vaccinium Species 0.000 description 1
- 229920006266 Vinyl film Polymers 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 239000002535 acidifier Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 description 1
- 235000010081 allicin Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 235000019994 cava Nutrition 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000004567 concrete Substances 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000024346 drought recovery Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- DYEQPYSFRWUNNV-APIJFGDWSA-N eudesmane Chemical compound C1CC[C@@H](C)[C@@H]2C[C@H](C(C)C)CC[C@]21C DYEQPYSFRWUNNV-APIJFGDWSA-N 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000007983 food acid Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229940047650 haemophilus influenzae Drugs 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002686 phosphate fertilizer Substances 0.000 description 1
- 229920006327 polystyrene foam Polymers 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of matrix and hardening off method for blueberry hardening, wherein hardening matrix includes water planting matrix and earth culture matrix, water planting matrix includes sterilization solvent, the tune sour solvent of 1 mass parts, the getting fat solvent of 1 mass parts, the solvent of taking root of 1 mass parts of 1 mass parts, earth culture matrix includes the composite soil of 1200~1300 parts by weight, the sulphur of the olive kernel leaf of 0.3~0.4 parts by weight, 0.3~0.35 parts by weight, the compost quick composting agent of 0.2~0.3 parts by weight.Its hardening off method is to take out the tissue-cultured seedling in blake bottle, is placed on moss package in hardening hole tray；Hardening hole tray is placed on hardening 28 days~35 days in the pond containing water planting matrix；Hardening hole tray is placed into hardening 4~6 months in earth culture matrix.The hardening off method of the present invention can reduce blueberry and disease probability occurs, and survival rate significantly improves.
Description
Technical field
The present invention relates to agricultural technology field, it particularly relates to which a kind of matrix for blueberry hardening, further relates to use
The method that the water planting matrix and earth culture matrix carry out blueberry hardening.
Background technology
Blueberry, Ericaceae Vaccinium, evergreen or machaka.Blueberry is rich in anthocyanidin, unrighted acid, tan
The elements such as acid and calcium, potassium, zinc, iron are spent, B family vitamin has very high healthcare function, and it is big strong to be classified as five by international food and agricultural organization
One of health food.According to cultivar origin, plant characteristic, area distribution, blueberry can be divided into Vaccinium ashei, southern high clump blueberry, north
Height is from 5 blueberry, short clump blueberry Breeds, and " outstanding rabbit " blueberry is one of Representative Cultivars of Vaccinium ashei, and drought tolerance is extremely strong,
Southern plantation is particularly suitable for, market promotion prospect is good.
Blueberry tissue culture seedling is sterile bottle inner ring in laboratory by Initial culture, squamous subculture, culture of rootage
Border.Hardening is tamed, and tames initial stage, should be similar to laboratory condition during culture, tames the later stage, then will be with estimated cultivation item
Part is similar, achievees the purpose that progressively to adapt to from interior to outdoor, improves the hardening survival rate of blueberry tissue culture seedling.
As people understand blueberry, plantation enthusiasm is progressively surging, and supply falls short of demand for seedling resource, and tissue culture expanding propagation gesture is must
Row, due to blueberry during hardening, it is necessary to adapt to be transitioned into outdoor environmental condition out of bottle, and found in this incubation
Blueberry hardening survival rate is low, is only 59%.
How the survival rate of blueberry hardening is improved into the technical issues of the urgent need solution of current blueberry tissue culture field.
The content of the invention
For the above-mentioned technical problem in correlation technique, the present invention proposes that a kind of matrix and blueberry for blueberry hardening is refined
Seedling-growing method can reduce blueberry and disease probability occurs, and survival rate has been increased to 92% from 59%.
To realize above-mentioned technical purpose, the technical proposal of the invention is realized in this way：
A kind of matrix for blueberry hardening, including water planting matrix and earth culture matrix, the water planting matrix includes 1 mass parts
Sterilization solvent, the tune sour solvent of 1 mass parts, the getting fat solvent of 1 mass parts, the solvent of taking root of 1 mass parts, the sterilization solvent
Garlic including 4~5 mass parts, the argy wormwood of 3~4 mass parts, the Phytolacca acinosa of 1~2 mass parts, 2~3 mass parts shoulder plate return, 2~
The Sculellaria barbata of 3 mass parts, the lindera glauca of 4~5 mass parts；The earth culture matrix includes the composite soil of 1200~1300 parts by weight,
The sulphur of the olive kernel leaf of 0.3~0.4 parts by weight, 0.3~0.35 parts by weight, the compost quick composting agent of 0.2~0.3 parts by weight.
The above-mentioned matrix for blueberry hardening, it is preferred that the spinach that acid solution is adjusted to include 2000~3000 parts by weight
The ammonium sulfate of trailing plants extracting solution, the fumaric acid of 3~4 parts by weight and 80~100 parts by weight.
The above-mentioned matrix for blueberry hardening, it is preferred that the getting fat solvent includes the day of 0.375g/L~0.4g/L
Right marine algae extract, the algal polysaccharides of 0.2g/L~0.3g/L, the amino polypeptide of 2.5g/L~3g/L, 1.5g/L~2/L
P2O5, the calcium of 1.25g/L~1.5g/L, the magnesium of 0.1g/L~0.2g/L, the zinc of 2.5g/L~3g/L, 2.25g/L~2.5g/L
Boron, 0.05g/L~0.06g/L EDTA-Fe, 0.0175g/L~0.02g/L EDTA-Cu, 0.00125g/L~0.0015/L
EDTA-Mo, 0.0005g/L~0.0006g/L EDTA-Co；The potassium dihydrogen phosphate of 20g/L.
The above-mentioned matrix for blueberry hardening, it is preferred that the brassin of the solvent including 0.00186g/L of taking root,
The gibberellin of 0.81g/L, the heteroauxin of 0.00312g/L.
The above-mentioned matrix for blueberry hardening, it is preferred that the composite soil includes turfy soil, laterite, perlite, sawmilling
Bits and pine needle, the turfy soil, laterite, perlite, the volume ratio of wood sawdust and pine needle for 3~4: 1~2: 2~3: 1~2: 1~
2。
The technical concept total as one, the present invention also provides a kind of using the above-mentioned matrix progress for blueberry hardening
The method of blueberry hardening, comprises the following steps：
S1, the tissue-cultured seedling in the blake bottle is taken out, is placed on moss package in hardening hole tray；
S2, hardening hole tray is placed on hardening 28 days~35 days in the pond containing water planting matrix；
S3, hardening hole tray is placed into hardening 4~6 months in earth culture matrix.
Above-mentioned method, it is preferred that the tissue-cultured seedling in the blake bottle is moved to from illumination cultivation room in greenhouse, bottle cap
0.5~1d of hardening is sealed, unclamps 0.5~1d of bottle cap hardening.
Above-mentioned method, it is preferred that moss described in the S1 steps wraps up tissue-cultured seedling again after being pre-processed, described pre-
Processing method is：First with 121 DEG C of high-pressure sterilizing pot retort sterilization 60 minutes, after cooling, then with the 2000 times of dilutions of first frost hymexazol
Liquid impregnates 15~30min.
Above-mentioned method, it is preferred that the S2 steps specifically include following steps：
S2~1 adds in the water planting matrix that depth is 0.06m in pond, and hardening hole tray is placed in water planting matrix, with poly-
Vinyl film, which is covered in, forms closed Small plastic shed on pond；
S2~2, water planting 1~7d of hardening, the film on Small plastic shed are totally-enclosed；
S2~3, water planting 8~15d of hardening, raise the film of Small plastic shed both sides diagonally opposing corner, are allowed to cross-ventilation；And it uses
Agricultural atomizer is sprayed once every 2h, keeps blade face moistening；
S2~4, water planting 16~28d of hardening, throw off the canopy film on Small plastic shed completely, later spray form water 2 times daily；Often
Spray within 12 days 1500 times of Mancozeb dilution 1~2 time.
Above-mentioned method, it is preferred that in the step of the S2~1, the polyethylene film thickness is 0.06mm, is existed with toothpick
It drills on polyethylene film, pitch of holes 5cm.
Above-mentioned method, it is preferred that during the S2 steps carry out, in greenhouse, hardening is to scatter based on light, often
It is 60% that keep greenhouse shading rates in the at 9 points in its morning at 4 points in afternoon, remaining time opens sunshade net, intensity of illumination 30001x~
40001x, 10 DEG C~30 DEG C of temperature, humidity 70~80%.
Above-mentioned method, it is preferred that the S3 steps specifically include following steps：
S3~1 is placed in hardening hole tray is smooth in earth culture matrix, and hanging wrap board cannot be arranged at bottom；
S3~2, every 2~3d waterings once, using spray form head, are not easy to rush and askew wash upside down seedling；Every two weeks with 0.2%
Potassium dihydrogen phosphate fertilizer sprays a blade face；Every two weeks once root is poured with 0.3% compound potassium sulfate fertilizer；A fungicide is sprayed per 40d,
Fungicide is thiophanate-methyl, pyrazoles ethofenprox is used alternatingly.
Above-mentioned method, it is preferred that 2 layers of sunshade net are installed at the top of the greenhouse, towards one layer of outdoor sunshade net
Using polyethylene sunshade net, shading rate 60%；Indoor sunshade net uses aluminium foil sunshade net, shading rate 70%；Polyethylene
Sunshade net leaves the ventilation of 1.5m with greenhouse top surface.
Beneficial effects of the present invention：
(1) the present invention provides a kind of matrix for blueberry hardening, wherein earth culture matrix includes 1200~1300 weight
The composite soil of part, the olive kernel leaf of 0.3~0.4 parts by weight, the sulphur of 0.3~0.35 parts by weight, the compost of 0.2~0.3 parts by weight
Quick composting agent.Olive kernel leaf rots that the substances such as substantial amounts of tannic acid, oxalic acid, humic acid and folic acid can be released, therefore has drop
Acid and the effect of sterilization.The acidifying of sulphur is a slow process, on the one hand acid is constantly lost in watering/process of raining, one
Constantly oxidation generation acid, the process can continue 3 years to aspect sulphur again, and pH maintains the sour environment of pH4~5.Most of bacterium
Optimum is grown under the neutral environment of pH6~8, and the reproduction speed under the sour environment of pH4~5 will slow down, therefore,
The infection of bacterium can effectively be prevented using the hardening matrix of the application, improve the survival rate of hardening.Compost quick composting agent is to use
In more quickly rotten wood sawdust and pine needle because organic fertilizer all must it is fully decomposed after could use, it is not so easy to be infested,
Especially insect pest-grub, and natural fermented decomposed process can be shortened to 2~3 by the agent of compost quick composting from 5~6 months
A month.Therefore, earth culture matrix of the invention is used for the matrix of blueberry hardening, can effectively improve the survival rate of blueberry hardening.
(2) the present invention provides a kind of matrix for blueberry hardening, the ingredient of the wherein bactericidal liquid of water planting matrix includes
Garlic, argy wormwood, Phytolacca acinosa, shoulder plate return, Sculellaria barbata, lindera glauca.Sulfur-containing compound has strong antibacterial and anti-inflammation functions in garlic, right
A variety of coccuses, bacillus, fungi and virus etc. have inhibition and killing effect, are that antibacterial action is most in the natural plants currently found
Strong one kind.The substances such as element are drawn containing substantial amounts of allicin, garlic in garlic, are the Fungicidal substances of natural strength, are had great
Bactericidal effect.The chemical composition of argy wormwood mainly has volatile oil, flavones, eudesmane, triterpenes and trace chemical elements etc., and argy wormwood exists
In vitro to bacillus anthracis, α-hemolytic streptococcus, beta hemolytic streptococcus, corynebacterium diphtheriae, Diplococcus pneumopniae, golden yellow Portugal
Grape coccus, hay bacillus etc. have inhibitory action.Extraction obtains a kind of antiviral glycoprotein (PAP) in Phytolacca acinosa, has anti-plant
Object virus and animal virus activity, while also have certain inhibitory action to the activity of fungi and bacterium.Plate is shouldered to return with certain
Antibacterium and antiviral effect, the antibacterial potency to will he, Si Mici, Fu Shi and bacterium sonnei is respectively 1:
512,1: 128,1: 64,1: 15.In addition, shoulder plate return to staphylococcus aureus, beta streptococcus, bacillus anthracis, corynebacterium diphtheriae,
Hay bacillus, typhoid bacillus, Pseudomonas aeruginosa and haemophilus influenzae etc. also have stronger antibacterial action.The antiviral examination outside chicken embryo
It tests, it is respectively 1: 160 and 1: 64 to shoulder plate to return to the antiviral activity of Asia influenza A virus and parainfluenza I type viruses.Half
Lotus has inhibitory action to staphylococcus aureus, shigella flexneri, typhoid bacillus, Pseudomonas aeruginosa, Escherichia coli.Equally, mountain
The bactericidal effect of pepper is also very strong.The application by garlic, argy wormwood, Phytolacca acinosa, shoulder plate return, Sculellaria barbata, lindera glauca according to mass ratio be 4
~5: 3~4: 1~2: 2~3: 2~3: 4~5 mixing, 6 kinds of ingredients complement each other, and can effectively prevent the infection of bacterium, improve refining
Seedling survives.
(3) the present invention provides a kind of matrix for blueberry hardening, the wherein tune acid solution of water planting matrix includes 2000
The ammonium sulfate of the pineapple extracting solution of~3000 parts by weight, the fumaric acid of 3~4 parts by weight and 80~100 parts by weight.Most of bacterium
Optimum is grown under the neutral environment of pH6~8, and the reproduction speed under the sour environment of pH4~5 will slow down, and
Blueberry likes the acidic growth matrix environment of ph4~5, therefore the present invention adds in water planting matrix and adjusts acid solution, ensures water planting
The pH of matrix is also maintained under the sour environment of pH4~5, can effectively prevent the infection of bacterium, improves the survival rate of hardening.Rich horse
Acid is a kind of organic acid, and acidity regulator, acidulant, anti-oxidant auxiliary agent, marinated accelerating agent, fragrance can be used as to use.And to suppression
Bacterium is mould proof to play an important role.Fumaric acid is that China GB2760-1996 provides to allow the food acid degree conditioning agent used, acidifying
Agent, anti-oxidant auxiliary agent, marinated accelerating agent and flavorant.Ammonium sulfate, blueberry like ammonium nitrogen, absorb the ability of ammonium nitrogen than inhaling
It is much better than to receive nitrate nitrogen.And ammonium sulfate is exactly ammonium nitrogen, ammonium sulfate is that acidity is that strong acid weak base salt, ammonium root are weak base.Because sulphur
Sour hydrogen radical stablize not facile hydrolysis, ammonium root hydrolysis can be inhibited, in acidity, about PH5.2~5.5, it has ammonium sulfate solution simultaneously
Deacidification, the effect of getting fat.
(4) the present invention provides a kind of method of blueberry hardening, first water planting, then earth culture, making it, progressively transition and adaptation are outer
Boundary's environment, in sour environment lower refining seedling, sufficient sterilizing measure reduces disease probability, and survival rate is improved from 59%
To 92%.Tissue-cultured seedling is environment in sterile bottle in laboratory by Initial culture, squamous subculture, culture of rootage.It is blue
The certain kind of berries is during hardening, it is necessary to adapt to be transitioned into outdoor environmental condition out of bottle.For this purpose, blueberry is first carried out water planting by the application
Culture, it is similar to the laboratory condition during culture when condition of water planting culture just starts, gradually to cultivation condition transition, finally adopt
With earth culture culture, reach the requirement of cultivation condition, achieve the purpose that progressively to adapt to from interior to outdoor, improve blueberry tissue culture seedling
Hardening survival rate.
(5) the present invention provides a kind of method of blueberry hardening, it is found by the applicant that bacterial infection, humidity are inadequate, matrix PH
Value not up to standard is training tissue culture seedling main causes of death.The present invention carries out blueberry hardening process in greenhouse, greenhouse
Top 2 layers of sunshade net are installed, the shading rate of outdoor polyethylene sunshade net is 60%, the shading rate of indoor aluminium foil sunshade net
For 70%.During summer hardening, open external sunshade net, the radiations heat energy of the sun and ultraviolet light be blocked in outdoor, external sunshade net with
Greenhouse top surface leaves the ventilation strip of 1.5m, and the air of flowing takes away the heat of absorption, reduces and conducts indoor heat, reduces temperature
Degree.During winter hardening, internal sunshade net is opened, heat preservation and heat absorption capacity using aluminium foil improve the indoor temperature of temperature.Meanwhile water
During training, relatively thin polyethylene film is selected, thickness 0.06mm increases its daylighting, makes in shed that liquid is water to be
Spray water increases in canopy humidity to 70~80%.Film is drilled with toothpick, and pitch of holes 5cm increases its gas permeability, especially suitable
During for summer hardening, 2~4 degree of temperature in film shed can be reduced, high temperature heat wave is avoided in shed to burn seedling.
(6) the present invention provides a kind of method of blueberry hardening, tissue-cultured seedling is wrapped up with moss, the benefit of moss is：1st, just
Preferably.2nd, for a long time, tissue-cultured seedling is most afraid of water shortage, and water could promote to grow more roots for water conservation moisturizing.3rd, moss has hole, dredges
Pine breathes freely, provides space for the breathing and growth of root, blueberry likes draining good, ventilated environment.4th, dry moss can be with
It is long-term to preserve, but often wet moss of the watering in moisture state, generally rotted to tail off gradually at the 5th~7 month, what corruption became
Process just provides nutrient to blueberry seedling, and the seedling that moss encloses, generally at 6~8 months, when growing to 12cm~13cm,
The nutritive cube just it being transplanted to out of greenhouse in greenhouse.
Description of the drawings
It in order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the present invention
Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings
Obtain other attached drawings.
Fig. 1 is the water planting process picture described according to embodiments of the present invention 3.
Fig. 2 is the earth culture process picture described according to embodiments of the present invention 3.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art's all other embodiments obtained belong to what the present invention protected
Scope.
In order to facilitate understand the present invention above-mentioned technical proposal, below by way of in specifically used mode to the present invention it is above-mentioned
Technical solution is described in detail.
Material and instrument employed in following embodiment are commercially available.
Embodiment 1：
A kind of water planting matrix for blueberry hardening, the tune sour solvent of sterilization solvent, 1 mass parts including 1 mass parts, 1
The getting fat solvent of mass parts, the solvent of taking root of 1 mass parts.
It wherein sterilizes solvent and includes the garlic of 5 mass parts, the argy wormwood of 3 mass parts, the Phytolacca acinosas of 2 mass parts, 3 mass parts and shoulder
Plate is returned, the Sculellaria barbata of 2 mass parts, the lindera glauca of 5 mass parts；
Sour solvent is adjusted to include pineapple extracting solution, the fumaric acid of 4 parts by weight and the sulfuric acid of 100 parts by weight of 2000 parts by weight
Ammonium.
It is more that getting fat solvent includes the natural seaweed extract of 0.375g/L, the algal polysaccharides of 0.2g/L, the amino of 2.5g/L
The P of peptide, 1.5g/L2O5, the calcium of 1.25g/L, the magnesium of 0.1g/L, the zinc of 2.5g/L, the boron of 2.25g/L, the EDTA- of 0.05g/L
The EDTA-Co of EDTA-Mo, 0.0005g/L of EDTA-Cu, 0.00125g/L of Fe, 0.0175g/L；The biphosphate of 20g/L
Potassium (content of potassium dihydrogen phosphate is more than 98%).
Solvent of taking root includes the brassin of 0.00186g/L, the gibberellin of 0.81g/L, the heteroauxin of 0.00312g/L.
A kind of preparation method of the water planting matrix for blueberry hardening of the present embodiment, specifically includes following steps：
(1) sterilization solvent is prepared：
1.1st, take 300g argy wormwoods, 200g Phytolacca acinosas, 300g shoulder plate return, 200g Sculellaria barbatas, 500g lindera glaucas, be put into pot, by 1
: 3 material water weight ratio adds water, after impregnating 0.5h, boils by intense fire, then is endured 30 minutes with slow boiling, with 50 mesh standard sieve mistakes after cooling
Filter obtains the first extracting solution.
1.2nd, garlic using broken wall cooking machine is blended, adds water by 1: 2 material water weight ratio, when extraction 2 is small at room temperature,
100 mesh standard sieves filter, and obtain the second extracting solution.
1.3rd, the first extracting solution and the second extracting solution are mixed, is sealed under the conditions of low-temperature dark.
(2) prepare and adjust sour solvent：
2.1st, 500g pineapples are taken, add water 2kg, at room temperature extract 24 it is small when after filter, obtain 2000g pineapple extraction
Liquid.
2.2nd, 4g fumaric acid (fumaric acid) is added in pineapple extracting solution, (total nitrogen of ammonium sulfate contains 100g ammonium sulfate
Amount, which is more than 21%), to be uniformly mixed, obtains the tune sour solvent that pH value is 4~5.
(3) getting fat solvent is prepared：Take 50ml nutrient solutions (the natural seaweed extract containing 15g/L in nutrient solution, 8g/L
Algal polysaccharides, the amino polypeptide of 100g/L, the P of 60g/L2O5, the calcium of 50g/L, the magnesium of 4g/L, the zinc of 100g/L, 90g/L boron,
The EDTA-Co of EDTA-Mo, 0.02g/L of EDTA-Cu, 0.05g/L of EDTA-Fe, 0.7g/L of 2g/L), 40g biphosphates
Potassium (content of potassium dihydrogen phosphate is more than 98%), adds water 2kg, mixes, the same day is with use.
(4) solvent of taking root is prepared：Take 12g it is green shield (in green shield containing 0.00031% brassin, 0.135% gibberellin,
0.00052% heteroauxin), be watered 2kg, the same day with mix with.
(5) it is 1: 1: 1: 1 mixing according to mass ratio by sterilization solvent, tune sour solvent, getting fat solvent and solvent of taking root, obtains
For the water planting matrix of blueberry hardening.
Embodiment 2：
A kind of earth culture matrix for blueberry hardening, including 1300 parts by weight composite soils, the olive kernel leaf of 0.3 parts by weight,
The sulphur of 0.35 parts by weight, (compost quick composting agent is purchased from Guangxi and helps agriculture livestock technology for the compost quick composting agent of 0.2 parts by weight
Co., Ltd, name of product：Big ratio is big).Wherein composite soil includes turfy soil, laterite, perlite, wood sawdust, pine needle, volume
Than for 4: 2: 3: 2: 1.
The preparation method of the earth culture matrix for blueberry hardening of the present embodiment, comprises the following steps：
(1) composite soil is prepared：By turfy soil, laterite, perlite, wood sawdust and pine needle according to volume ratio be 4: 2: 3: 2: 1
It is mixed to get composite soil.
(2) per 1m3Composite soil addition 300g olive kernel leafs, the sulphur of 350g, 200g compost quick composting agent, after uniform mixing
Plastic covering film fermentation maturity 3 months, treats that acidic materials fully chemically react, pH value is made to be down to 4~5, obtains for blueberry
The earth culture matrix of hardening.
Embodiment 3：
A kind of water planting matrix of embodiment 1, application of the earth culture matrix of embodiment 2 in blueberry hardening, the present embodiment
Blueberry tissue culture seedling uses " outstanding rabbit " kind.Its application process comprises the following steps：
(1) preparation：
1st, the selection of tissue-cultured seedling：The tissue-cultured seedling for select robust growth, growing fine, the plant height of bottle outlet hardening for 8~
10cm.The tissue-cultured seedling of select is moved to greenhouse by (plant height refers to height of the tissue-cultured seedling from terminal bud to root) from illumination cultivation room
In greenhouse, bottle cap sealing hardening 1d unclamps bottle cap hardening 1d, is allowed to progressively adapt to outdoor environment.
2nd, the preparation of hardening hole tray：The polystyrene foam plate in 200 caves is taken, sealed bottom connection is integral, first with wooden chopsticks
Bottom is exposed, is allowed to run through up and down, as hardening hole tray.Reuse 1000 times of potassium permanganate liquid soaking disinfection 10 minutes.
3rd, the pretreatment of moss：Moss is taken, first with 121 degree of high-pressure sterilizing pot retort sterilization 60 minutes, after cooling, then uses first
2000 times of dilution liquid of white hymexazol impregnate the tongue after 20min (soaking time for 15~30 minutes can) moss is sterilized
Moss.
4th, the design in greenhouse：2 layers of sunshade net are installed at the top in greenhouse, and polyethylene sunshade is used towards one layer of outdoor sunshade net
Net, shading rate 60%；Indoor sunshade net uses aluminium foil sunshade net, shading rate 70%.Polyethylene sunshade net and greenhouse top
The ventilation strip of 1.5m is left in face, and the air of flowing takes away the heat of absorption, reduces and conducts indoor heat, reduces temperature.
The rectangle pond that the indoor cement concrete of temperature is built by laying bricks or stones, for south-north direction array in greenhouse, long 4m is wide
1.2m, high 0.2m.The bow member that height is 70cm is built on pond, the thickness that water white transparency is covered on bow member is the poly- second of 0.06mm
Alkene film is formed.Film on Small plastic shed is drilled with toothpick, and pitch of holes 5cm increases its gas permeability.
(2) tissue-cultured seedling is taken out from blake bottle with tweezers, distilled water thoroughly cleans the culture medium that root carries, Yi Mianfa
It is mould.Distilled water immersion tissue-cultured seedling is used again, in case tender leaf water shortage is withered.The moss after appropriate disinfection is taken, single tissue-cultured seedling is wrapped up
At moss most middle part, one plant of tissue-cultured seedling is inserted in the cavities of each hardening hole tray, plant base portion will slightly expose, and root system need to the group of breaking
Stretching, extension.
(3) water planting hardening：After rectangle pond cleaning in room temperature, the water planting matrix of embodiment 1 is added in, depth is
0.06m.The hardening hole tray dispensed is put into pond, each pond can put 8 plate hardening hole trays, by polyethylene film lap-joint
Water bonding is spread, overlap joint is formed compared with confined space.During water planting hardening, in greenhouse, hardening is to scatter based on light, per in the sky
It is 60% that 9 points of noon at 4 points in afternoon, which keeps greenhouse shading rates, remaining time opens sunshade net, intensity of illumination 30001x~40001x,
10 DEG C~30 DEG C of temperature, humidity 70~80%.
Water planting 1~7d of hardening, the film on Small plastic shed is totally-enclosed, simulates indoor environment.
Water planting 8~15d of hardening raises the film of Small plastic shed both sides diagonally opposing corner, is allowed to cross-ventilation, progressively ventilation
Adapt to external environment.Using agricultural atomizer every 2h spray forms water once, keep blade face moistening.A water planting is replaced per 10d
Matrix.
After 16~28d of water planting hardening, the canopy film on Small plastic shed is thrown off completely, later spray form water 2 times daily.Every 12 days
Spraying 1500 times of Mancozeb dilution 1~2 time, (winter in spring and autumn is 1 time, and the summer is 2 times, and it doesn't matter for spray form water and spray Mancozeb
Successively, after when but spray form water 2 is small, blade face quick-drying sprays Mancozeb again, and spray have to after Mancozeb interval 4 it is small when with
Spray form water again afterwards).Mancozeb is the protection fungicide of non-internal-absorting, can prevent a variety of leaf portion fungal diseases.
Fig. 1 is the picture of water planting process.
(4) earth culture hardening：Earth culture matrix disinfection is carried out in advance, and specific method is：Spray applies the 800 times of dilutions of first frost hymexazol
Liquid.By in the smooth earth culture matrix put after sterilization of hardening hole tray, notice that hanging wrap board cannot be arranged at bottom, otherwise easy dehydration is dead
Seedling.Every 2~3d watering once, using spray form head, is not easy to rush and askew washes upside down seedling.Every two weeks with 0.2% potassium dihydrogen phosphate
Blade face of fertilizer spray.Once root is poured with 0.3% compound potassium sulfate fertilizer every two weeks, the dusk time-division it is optimal in spraying effect.Per 40d
A fungicide is sprayed, fungicide is thiophanate-methyl, pyrazoles ethofenprox is used alternatingly.
Fig. 2 is the picture of earth culture process.
Healthy and strong blueberry moss seedling is obtained after four months, and is planted in transplanting to nutritive cube, hardening is completed, survival rate
For 92%.
Comparative example 1：
The blueberry hardening off method of a kind of comparative example, comprises the following steps：
The tissue-cultured seedling for select robust growth, growing fine.The tissue-cultured seedling of select is existed from illumination cultivation room directly transplanting
In earth culture matrix (the earth culture matrix of this comparative example is laterite).Small plastic shed is built above earth culture matrix and to cover layer of transparent thin
Film does not punch on film, cultivates 4 months.
Four months blueberry shoot survival percents are 59%.
Analysis comparison is understood：
(1) air humidity has a significant impact blueberry tissue culture transplantation of seedlings survival, hardening, comparison in pond in embodiment 3
Example 1 directly puts hardening in soil, and high in the humidity ratio soil in water, survival rate is also with regard to higher.
(2) during earth culture, transparent membrane does not punch on the Small plastic shed of comparative example 1, especially in summer hardening, film
Interior airtight, heating is sultry, and water quality fouls faster, and the death rate is high.
(3) in comparative example 1, soil thorough disinfection is difficult, and in embodiment 3, it is then simpler that fungicide is directly added in water
List and abundant sterilization, rigid connection touch extraneous tissue-cultured seedling, it is easy to which microbiological contamination death and in flakes infection are dead, and cystosepiment is placed on pond
In, there is fungicide in water, after partly being done a little on moss, water planting matrix will be sucked at the moss of infiltration in water, also
It is that bubble is in the environment containing fungicide when the tissue-cultured seedling for saying at this moment is exactly 24 small substantially, so water quality fouls slower, microbiological contamination is more
It is few.
(4) most of bacterium optimum is grown under the neutral environment of pH6~8, and under the sour environment of pH 4~5
Reproduction speed will slow down, therefore just added in during the water planting of embodiment 3 and adjust acid solution.
Embodiment 4：
Prepare five kinds of earth culture matrix respectively：
Earth culture matrix 1：The earth culture matrix of embodiment 2.
Earth culture matrix 2：Composite soil is replaced with into laterite, remaining is consistent with the earth culture matrix of embodiment 2.
Earth culture matrix 3：Not comprising olive kernel leaf, remaining ingredient is consistent with the earth culture matrix of embodiment 2.
Earth culture matrix 4：Not comprising sulphur, remaining ingredient is consistent with the earth culture matrix of embodiment 2.
Earth culture matrix 5：Not comprising compost quick composting agent, remaining ingredient is consistent with the earth culture matrix of embodiment 2.
Blueberry tissue culture seedling is cultivated according to the method for embodiment 3, investigates the survival rate after the completion of hardening, investigates result
Referring to table 1.
Table 1：Different earth culture matrix are to the influence result table of blueberry connection shoot survival percent
| Group | Earth culture matrix 1 | Earth culture matrix 2 | Earth culture matrix 3 | Earth culture matrix 4 | Earth culture matrix 5 |
| Survival rate % | 92% | 65% | 79% | 76% | 81% |
As can be known from the results of Table 1：Different earth culture matrix have certain influence to the survival rate of blueberry hardening, using embodiment
2 earth culture matrix is compared to other earth culture matrix, to the survival rate higher of blueberry hardening.
Embodiment 5：
Prepare four kinds of water planting matrix respectively：
Water planting matrix 1：The water planting matrix of embodiment 1.
Water planting matrix 2：Germicidal solution is pyraclostrobin fungicide, remaining ingredient and the water planting discrete phase of embodiment 1 are same.
Water planting matrix 3：Tune acid solution is citric acid solution, remaining ingredient is same as Example 1.
Water planting matrix 4：Germicidal solution be pyraclostrobin fungicide, tunes acid solution be citric acid solution, remaining ingredient and
Embodiment 1 is identical.
Water planting matrix 5：Take 12g it is green shield (in green shield containing 0.00031% brassin, 0.135% gibberellin,
0.00052% heteroauxin), it is watered 2kg.
Blueberry tissue culture seedling is cultivated according to the method for embodiment 3, investigates the survival rate after the completion of hardening, investigates result
Referring to table 2.
Table 2：Different water planting matrix are to the influence result table of blueberry connection shoot survival percent
| Group | Water planting matrix 1 | Water planting matrix 2 | Water planting matrix 3 | Water planting matrix 4 | Water planting matrix 5 |
| Survival rate % | 92% | 79% | 81% | 71% | 62% |
As can be known from the results of Table 2：Different water planting matrix have certain influence to the survival rate of blueberry hardening, using embodiment
1 water planting matrix, to the survival rate highest of blueberry hardening.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modifications, equivalent replacements and improvements are made should all be included in the protection scope of the present invention god.
Claims (10)
1. a kind of matrix for blueberry hardening, which is characterized in that including water planting matrix and earth culture matrix, the water planting matrix bag
Include sterilization solvent, the tune sour solvent of 1 mass parts, the getting fat solvent of 1 mass parts, the solvent of taking root of 1 mass parts of 1 mass parts, institute
Stating sterilization solvent includes the garlic of 4~5 mass parts, the argy wormwood of 3~4 mass parts, the Phytolacca acinosas of 1~2 mass parts, 2~3 mass parts
Shoulder plate return, the Sculellaria barbata of 2~3 mass parts, the lindera glauca of 4~5 mass parts；The earth culture matrix includes 1200~1300 parts by weight
Composite soil, the olive kernel leaf of 0.3~0.4 parts by weight, the sulphur of 0.3~0.35 parts by weight, the compost of 0.2~0.3 parts by weight is fast
Fast decomposing agent.
It is 2. according to claim 1, which is characterized in that for the matrix of blueberry hardening, which is characterized in that described to adjust acid molten
Liquid includes pineapple extracting solution, the fumaric acid of 3~4 parts by weight and the ammonium sulfate of 80~100 parts by weight of 2000~3000 parts by weight；
And/or the getting fat solvent includes the natural seaweed extract of 0.375g/L~0.4g/L, the sea of 0.2g/L~0.3g/L
Polysaccharides, the amino polypeptide of 2.5g/L~3g/L, the calcium of P2O5,1.25g/L~1.5g/L of 1.5g/L~2/L, 0.1g/L~
The magnesium of 0.2g/L, the zinc of 2.5g/L~3g/L, the boron of 2.25g/L~2.5g/L, 0.05g/L~0.06g/L EDTA-Fe,
EDTA-Mo, 0.0005g/L of EDTA-Cu, 0.00125g/L of 0.0175g/L~0.02g/L~0.0015/L~0.0006g/
The EDTA-Co of L；The potassium dihydrogen phosphate of 20g/L；
And/or the solvent of taking root includes the brassin of 0.00186g/L, the gibberellin of 0.81g/L, the indoles of 0.00312g/L
Acetic acid.
3. the matrix according to claim 1 for blueberry hardening, which is characterized in that the composite soil include turfy soil,
Laterite, perlite, wood sawdust and pine needle, the turfy soil, laterite, perlite, the volume ratio of wood sawdust and pine needle are 3~4: 1
~2: 2~3: 1~2: 1~2.
4. a kind of method that blueberry hardening is carried out using the matrix that blueberry hardening is used for any one of claims 1 to 3,
It is characterized in that, comprises the following steps：
S1, the tissue-cultured seedling in the blake bottle is taken out, is placed on moss package in hardening hole tray；
S2, hardening hole tray is placed on hardening 28 days~35 days in the pond containing water planting matrix；
S3, hardening hole tray is placed into hardening 4~6 months in earth culture matrix.
5. according to the method described in claim 4, it is characterized in that, moss is wrapped again after being pre-processed described in the S1 steps
Tissue-cultured seedling is wrapped up in, the preprocess method is：First with 121 DEG C of high-pressure sterilizing pot retort sterilization 60 minutes, after cooling, then disliked with first frost
Mould clever 2000 times of dilutions impregnate 15~30min.
6. according to the method described in claim 4, it is characterized in that, the S2 steps specifically include following steps：
S2~1 adds in the water planting matrix that depth is 0.06m in pond, and hardening hole tray is placed in water planting matrix, uses polyethylene
Film, which is covered in, forms closed Small plastic shed on pond；
S2~2, water planting 1~7d of hardening, the film on Small plastic shed are totally-enclosed；
S2~3, water planting 8~15d of hardening, raise the film of Small plastic shed both sides diagonally opposing corner, are allowed to cross-ventilation；And using agricultural
Sprayer once, keeps blade face moistening every 2h spray forms water；
S2~4, water planting 16~28d of hardening, throw off the canopy film on Small plastic shed completely, later spray form water 2 times daily；Every 12 days
Spray 1500 times of Mancozeb dilution 1~2 time.
7. according to the method described in claim 6, it is characterized in that, the S2~and 1 in step, the polyethylene film thickness is
0.06mm is drilled with toothpick on polyethylene film, pitch of holes 5cm.
8. according to the method described in claim 4, it is characterized in that, the S2 steps carry out during, in greenhouse, hardening
To scatter based on light, it is 60% that 9 points of every morning at 4 points in afternoon, which keeps greenhouse shading rates, remaining time opens sunshade net, illumination
Intensity 3000lx~4000lx, 10 DEG C~30 DEG C of temperature, humidity 70~80%.
9. according to the method described in claim 4, it is characterized in that, the S3 steps specifically include following steps：
S3~1 is placed in hardening hole tray is smooth in earth culture matrix, and hanging wrap board cannot be arranged at bottom；
S3~2, every 2~3d waterings once, using spray form head, are not easy to rush and askew wash upside down seedling；Every two weeks with 0.2% phosphoric acid
Dihydro potash fertilizer sprays a blade face；Every two weeks once root is poured with 0.3% compound potassium sulfate fertilizer；A fungicide, sterilization are sprayed per 40d
Agent is thiophanate-methyl, pyrazoles ethofenprox is used alternatingly.
10. according to the method described in claim 4, it is characterized in that, 2 layers of sunshade net, court are installed in the top of the greenhouse
One layer of outdoor sunshade net is using polyethylene sunshade net, shading rate 60%；Indoor sunshade net uses aluminium foil sunshade net, shading
Rate is 70%；Polyethylene sunshade net leaves the ventilation of 1.5m with greenhouse top surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711361352.XA CN108077022A (en) | 2017-12-18 | 2017-12-18 | For the matrix of blueberry hardening and blueberry hardening off method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711361352.XA CN108077022A (en) | 2017-12-18 | 2017-12-18 | For the matrix of blueberry hardening and blueberry hardening off method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108077022A true CN108077022A (en) | 2018-05-29 |
Family
ID=62176619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711361352.XA Pending CN108077022A (en) | 2017-12-18 | 2017-12-18 | For the matrix of blueberry hardening and blueberry hardening off method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108077022A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109076917A (en) * | 2018-07-12 | 2018-12-25 | 贵州高原蓝生态农业科技开发有限公司 | A kind of matrix and blueberry hardening off method for blueberry hardening |
| CN114208514A (en) * | 2021-12-24 | 2022-03-22 | 广西壮族自治区中国科学院广西植物研究所 | High-survival-rate cutting seedling method for medicinal and edible plant litsea cubeba |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
| CN115428710A (en) * | 2022-09-22 | 2022-12-06 | 苏州大学 | A kind of aquatic plant planting module and manufacturing method thereof |
| CN116762681A (en) * | 2023-05-12 | 2023-09-19 | 中国热带农业科学院南亚热带作物研究所 | Hydroponic method for promoting pineapple seedlings to root |
| CN116982489A (en) * | 2023-08-21 | 2023-11-03 | 云南省农业科学院热带亚热带经济作物研究所 | Cutting seedling raising method for bergamot |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550376A (en) * | 2010-12-14 | 2012-07-11 | 金陵科技学院 | Summer exercising method for blueberry seedlings |
| CN102701876A (en) * | 2012-07-09 | 2012-10-03 | 绥化京绥生物工程有限公司 | Matrix special for tobacco soilless seedling and preparation method thereof |
| CN103460971A (en) * | 2013-09-30 | 2013-12-25 | 湖南省农业生物资源利用研究所 | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings |
| CN106187600A (en) * | 2016-07-13 | 2016-12-07 | 吉林省普蓝高科技有限公司 | 1 year raw seedling culture matrix of blue berry and preparation method thereof |
| CN106613859A (en) * | 2017-02-08 | 2017-05-10 | 合肥师范学院 | Cultivation method of sound seedlings of blueberry tissue culture seedlings |
| CN107258545A (en) * | 2017-07-29 | 2017-10-20 | 伊春林业科学院 | A kind of blueberry tissue culture bottle seedling fast numerous matrix and technology |
-
2017
- 2017-12-18 CN CN201711361352.XA patent/CN108077022A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550376A (en) * | 2010-12-14 | 2012-07-11 | 金陵科技学院 | Summer exercising method for blueberry seedlings |
| CN102701876A (en) * | 2012-07-09 | 2012-10-03 | 绥化京绥生物工程有限公司 | Matrix special for tobacco soilless seedling and preparation method thereof |
| CN103460971A (en) * | 2013-09-30 | 2013-12-25 | 湖南省农业生物资源利用研究所 | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings |
| CN106187600A (en) * | 2016-07-13 | 2016-12-07 | 吉林省普蓝高科技有限公司 | 1 year raw seedling culture matrix of blue berry and preparation method thereof |
| CN106613859A (en) * | 2017-02-08 | 2017-05-10 | 合肥师范学院 | Cultivation method of sound seedlings of blueberry tissue culture seedlings |
| CN107258545A (en) * | 2017-07-29 | 2017-10-20 | 伊春林业科学院 | A kind of blueberry tissue culture bottle seedling fast numerous matrix and technology |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109076917A (en) * | 2018-07-12 | 2018-12-25 | 贵州高原蓝生态农业科技开发有限公司 | A kind of matrix and blueberry hardening off method for blueberry hardening |
| CN114208514A (en) * | 2021-12-24 | 2022-03-22 | 广西壮族自治区中国科学院广西植物研究所 | High-survival-rate cutting seedling method for medicinal and edible plant litsea cubeba |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
| CN115428710A (en) * | 2022-09-22 | 2022-12-06 | 苏州大学 | A kind of aquatic plant planting module and manufacturing method thereof |
| CN116762681A (en) * | 2023-05-12 | 2023-09-19 | 中国热带农业科学院南亚热带作物研究所 | Hydroponic method for promoting pineapple seedlings to root |
| CN116982489A (en) * | 2023-08-21 | 2023-11-03 | 云南省农业科学院热带亚热带经济作物研究所 | Cutting seedling raising method for bergamot |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108077022A (en) | For the matrix of blueberry hardening and blueberry hardening off method | |
| CN102511333B (en) | Root-limiting direct-sowing tobacco seedling tray and root-limiting direct-sowing tobacco seedling raising method | |
| KR101431787B1 (en) | Organic Ginseng and the Method of Cultivation thereof | |
| CN107343445A (en) | Pseudo-wild cultivating method under dendrobium candidum pine forests | |
| CN102550252A (en) | Cultivation method of Dendrobium officinale | |
| CN105493944B (en) | A kind of blueberry seedling fosters matrix and preparation method thereof | |
| CN102960226B (en) | Dendrobium officinale Kimura et Migo tissue culture seedling acclimatization method | |
| CN105145313B (en) | A kind of implantation methods of dendrobium candidum | |
| CN105746323A (en) | Soilless culture method of organic cucurbita pepo L. | |
| CN106718735A (en) | A kind of method for planting dendrobium candidum in the tree | |
| CN110972864A (en) | Selenium-rich cultivation method for Bigheng white atractylodes rhizome | |
| CN106699423A (en) | Lycium barbarum root rot biocontrol preparation and preparation method thereof | |
| CN105746168A (en) | Imitated wild cultivation method for roxburgh anoectochilus terminal buds | |
| CN107484601A (en) | A kind of implantation methods for improving walnut volume increase | |
| CN107667758A (en) | A kind of implantation methods of blueberry | |
| CN105638300B (en) | A kind of method of shallot promoting root growth strong sprout | |
| CN108575752B (en) | A kind of rapid propagation method suitable for large-scale production Black Box Tracing high quality seedling | |
| CN105900662A (en) | Soilless half-hydroponic planting method of organic Chinese chives | |
| CN108184545A (en) | A kind of Resistant White mushroom culture material and preparation method thereof and application | |
| CN109832143A (en) | A kind of dendrobium candidum planting matrix and preparation method thereof | |
| CN106748362A (en) | A kind of method for planting dendrobium candidum in the tree | |
| KR100356762B1 (en) | Method to manufacture complex microbe culture and manure using complex microbe culture | |
| CN106234130A (en) | A kind of cultural method of flowering plum | |
| CN106688528A (en) | Method for planting high-nutrition pitaya | |
| CN105961160A (en) | Organic cucumber soilless planting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180529 |