CN115735584A - Lily scale cuttage method capable of improving cuttage propagation coefficient and bulb size - Google Patents
Lily scale cuttage method capable of improving cuttage propagation coefficient and bulb size Download PDFInfo
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- CN115735584A CN115735584A CN202211339301.8A CN202211339301A CN115735584A CN 115735584 A CN115735584 A CN 115735584A CN 202211339301 A CN202211339301 A CN 202211339301A CN 115735584 A CN115735584 A CN 115735584A
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- 241000234435 Lilium Species 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000005520 cutting process Methods 0.000 claims abstract description 32
- 238000002791 soaking Methods 0.000 claims abstract description 11
- 239000004927 clay Substances 0.000 claims abstract description 10
- 238000007598 dipping method Methods 0.000 claims abstract description 10
- 239000000758 substrate Substances 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 4
- 239000006013 carbendazim Substances 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 201000010099 disease Diseases 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 239000003415 peat Substances 0.000 claims description 2
- 239000003755 preservative agent Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims description 2
- 239000002002 slurry Substances 0.000 claims description 2
- 230000007480 spreading Effects 0.000 claims description 2
- 238000003892 spreading Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 229910052902 vermiculite Inorganic materials 0.000 claims description 2
- 235000019354 vermiculite Nutrition 0.000 claims description 2
- 239000010455 vermiculite Substances 0.000 claims description 2
- 238000009736 wetting Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract 1
- 238000004904 shortening Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 7
- 238000012258 culturing Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 240000008058 Lilium brownii Species 0.000 description 1
- 235000015982 Lilium brownii Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention relates to a lily scale cuttage method capable of improving cuttage propagation coefficient and bulb size, wherein scales are placed in PP before cuttage 333 Soaking in solution, and then soaking the scales in PP 333 Quick dipping treatment is carried out in clay prepared by the solution, then cuttage is carried out by using a buried-leaf cuttage mode, and dark culture is carried out on the scales after cuttage for 6090 days at the temperature of 25 ℃. The invention adopts PP 333 The lily scales are treated by the solution, the number of the small bulbs for cuttage is increased by 54.47-63.96 percent compared with the control group, and the circumference is increased by 8.17-30.77 percent; the lily cutting propagation methodThe breeding method has the characteristics of low cost, convenient operation, high propagation coefficient and the like, solves the technical bottleneck that the number and the circumference of the small bulbs in the scale cutting propagation of the lily bulb cannot be considered simultaneously, is favorable for the large-scale propagation of the lily bulb, and provides technical support for shortening the breeding period of the lily bulb and the domesticated production of the bulb.
Description
Technical Field
The invention relates to the technical field of plant propagation methods, in particular to a lily scale cuttage method.
Background
Lily (Lilium spp.) is a flower of bulb of autumn-planted lily of the genus Lilium of the family liliaceae, and is mainly distributed in the northern temperate zone. Lily is known as the king of bulbous flower, and the quality of seed bulbs is an important factor influencing the development of the Lily industry at home and abroad. The international lily breeding work is earlier researched, the international lily seedball monopoly market including the netherlands, the usa and japan is formed at present, the domestic lily seedball production is still in the development stage, the limited factors are more, and the problems of seedball degradation and the like generally exist. The propagation mode of lily includes sexual propagation and asexual propagation, wherein the asexual propagation is mainly used for production. Asexual propagation modes can be divided into scale cuttage propagation, bulbil propagation, bulb division propagation, tissue culture and the like. Researches show that the modes of plant division, bulb division and the like can cause the reduction of the comprehensive value of the lily and even the generation of toxicity; although the tissue culture method improves the breeding efficiency of the seedball, the production cost is higher, the requirements on related technologies are higher, and the popularization and the application are difficult to a certain extent. The scale cutting propagation is the most common method in the existing propagation mode, has high propagation coefficient, simple operation and low cost, and is convenient for popularization and large-scale production.
The traditional lily cuttage method is complicated and greatly influenced by seasons and temperature. Meanwhile, the conventional lily cuttage multi-shoot heavy propagation coefficient is improved, the relationship between the propagation coefficient and the circumference is neglected, so that bulblets generated by cuttage are small, and the field planting is needed for 3-4 years until the size of commodity balls is reached. Therefore, the lily cuttage technology is perfected, the cuttage propagation coefficient is improved, meanwhile, the growth of the bulblets is promoted, the lily bulb cultivation period can be obviously shortened, the technical foundation is laid for large-scale production of the lily bulbs, and the technical reserve is provided for localization of the lily bulbs.
Disclosure of Invention
According to the problems in the prior art, the invention discloses a lily scale cuttage method capable of improving the cuttage propagation coefficient and the bulb size, which has the advantages of high propagation coefficient, larger regenerated bulblet circumference, low propagation cost, convenient operation, suitability for various lily varieties and contribution to large-scale propagation of lily.
In order to solve the technical problems, the invention adopts the following technical scheme:
a lily scale cuttage method capable of improving cuttage propagation coefficient and bulb size comprises the following steps:
step 1, selecting healthy lily bulbs which are free of diseases and insects and are similar in size, peeling the outermost layer of scales, and disinfecting the outer layer of scales;
step 2, making the outer layer scale in PP with 50-100 mg/L 333 Soaking in the solution for 10-20 min, and then dipping the base of the lily scales with PP of 50-100 mg/L 333 Clay prepared from the solution;
step 3, preparing a cuttage matrix;
and 4, cutting the outer-layer scales treated in the step 2 into a cutting medium in a manner of embedding pieces into the cutting medium.
Further, PP in the step 2 333 The concentration of the solution was 100mg/L.
Further, in the step 2, PP 333 In solution-formulated clay, PP 333 The volume ratio of the solution to the clay was 1.
Further, the sterilization method in step 1 is as follows: soaking the scales in 50% carbendazim solution for 30-60 min, and spreading the scales on a preservative film for drying in the shade for 24h.
Further, in the step 2, the specific method for quickly dipping the outer layer scales after the outer layer scales are soaked comprises the following steps: on a culture dish, prepared PP is used 333 The solution and the viscous red sandy soil subjected to early sterilization are mixed according to the volume ratio of 1:1 stirring into a slurry shape, and dipping a small amount of clay at a position of 0.5-1.0 cm of the base part of the flake.
Further, the step 3 specifically includes: selecting the Pingshi peat soil and vermiculite according to the proportion of 1:1, uniformly mixing, and then wetting the substrate with a carbendazim solution until the substrate is in a state of being held into a mass and immediately dispersed after being thrown off, so as to achieve the effects of keeping the substrate wet and sterilizing the substrate.
Further, the step 4 specifically includes: placing a substrate with the thickness of 5-7 cm on the bottom layer of the cutting bag, obliquely inserting the pretreated outer-layer scale of the lily into the substrate with the concave surface facing upwards uniformly, placing the scale and the substrate at intervals, and covering a layer of substrate with the thickness of 5-7 cm on the uppermost side.
Further, in the step 4, after the scale cutting is finished, uniformly pricking a plurality of positions on the cutting bag every 2cm, and culturing in a dark room at 25 ℃.
The invention has the beneficial effects that: the invention puts the scale on PP before cuttage 333 Soaking in solution, and then soaking the scales in PP 333 The rapid dipping treatment is carried out in the clay prepared by the solution, the number of the cutting bulblets is increased by 54.47-63.96% compared with that of a control group, the circumference is increased by 8.17-30.77%, experiments prove that the circumference of the new bulblets is increased while the propagation coefficient is improved, the cost is low, the operation is convenient, the production period of the lily commodity bulbs is effectively shortened, the technical foundation is laid for the scale production of the lily bulbs, and meanwhile, the technical reserve is provided for the localization of the lily bulbs.
The present invention will be described in detail below with reference to the accompanying drawings and examples.
Drawings
FIG. 1 is a schematic flow chart of a lily scale cutting propagation system;
FIG. 2 is a diagram of regenerated bulblet after lily scales are cut for 90 days. (control: 0mg/L PP 333 (ii) a Processing one: 50mg/L PP 333 (ii) a And (5) processing: 100mg/L PP 333 (ii) a a 'wooden door' bulblet; b: 'siberia' bulblet).
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
FIG. 1 shows a specific process of the present invention.
EXAMPLE I Wood door Lily Scale cuttage experiment
1. The 'wooden door' lily is selected as an experimental material in the experiment, and the PP is selected as a reagent 333 Two concentration gradients were set, and a control run was set. Adding the scale on PP before cutting 333 Soaking in the solution for 10min, and quickly dipping the scales. Carrying out PP 333 The experiment of the solution on the regeneration of the lily bulblet is explored. The experimental treatment design is as in table 1.
TABLE 1PP 333 Solution treatment concentration gradient
And (5) performing result statistics on all the cutting scales after culturing for 90 days. The statistical indexes comprise the total number of bulblets, the propagation coefficient, the maximum circumference, the maximum fresh weight and the average fresh weight of the bulbs.
2. PP of different concentrations 333 Influence of solution on 'wooden door' lily scale cuttage propagation
As can be seen from Table 2 and FIG. 2a, two different concentrations of PP 333 The solution has a promoting effect on the scale cuttage propagation of the lilium brownii. The five index data of each treatment are higher than 0mg/L PP 333 Treatment in which the PP is 100mg/L 333 The processed propagation coefficient is most obvious compared with 0mg/L PP 333 The proliferation coefficient of the treatment increased by 63.96%. The most obvious increase of the maximum circumference and the maximum fresh weight is 50mg/L PP 333 The treatment, compared to the control, increased the circumference and fresh weight by 30.77% and 93.69%, respectively.
TABLE 2 statistics of wood door bulblet cuttage data
Example two, 'Siberian' lily bulb Scale cuttage experiments
1. The experiment selects 'Siberian' lily as an experimental material, and selects a reagent PP 333 Ladder with two kinds of concentrationAnd (4) setting a control test at the same time. The scales are placed on PP before cuttage 333 Soaking in the solution for 10min, and quickly dipping the scales. The experimental treatment design is as in table 1. And (5) performing result statistics on all the cutting scales after culturing for 90 days. The statistical indexes comprise the total number of bulblets, the propagation coefficient, the maximum circumference, the maximum fresh weight and the average fresh weight of the bulbs.
2. PP of different concentrations 333 Effect of the solution on the cutting propagation of Lily scales of Siberian As can be seen from Table 3 and FIG. 2b, two different concentrations of PP 333 The solution has promoting effect on scale cutting propagation of Bulbus Lilii of Siberian. Wherein the PP is 100mg/L 333 The processed propagation coefficient is most obvious compared with 0mg/L PP 333 The propagation coefficient of the treatment increased by 54.47%. The most obvious increase of the maximum circumference and the maximum fresh weight is 100mg/L PP 333 Treatment with 0mg/L PP 333 Compared with the treatment, the circumference and the fresh weight are respectively increased by 8.17 percent and 73.24 percent.
TABLE 3 statistics of siberia bulblet cuttage data
Integration of two concentrations of PP 333 Through the experiment of the solution on the scale cutting propagation of two varieties of lily on 'wooden door' and 'Siberian', the finding shows that the PP content is 100mg/L 333 The solution is 50mg/L PP 333 Is more beneficial to the cutting propagation of the lily scales and has obvious promotion effect on the propagation coefficient of the lily bulbs and the total fresh weight of the bulbs.
As can be seen from the two specific examples, the lily cuttage method provided by the invention can improve the propagation coefficient of lily scale cuttage, promote the expansion of the regenerated bulblets, and overcome the problem that the propagation coefficient and the bulb expansion of the bulblets in the conventional scale cuttage propagation cannot be considered at the same time. Through the treatment, the lily of the 'wooden door' variety is inserted in the wooden door to improve the propagation coefficient, meanwhile, the expansion of the new bulblets is promoted, and the number of the bulblets with larger circumference diameter larger than 6cm is obviously increased; and the diameter of the new bulblet after the scale cuttage of the Siberian is obviously increased no matter the propagation coefficient or the circumference of the new bulblet. Therefore, the invention can be proved to have the effect of improving the lily cuttage propagation coefficient and the circumference of the new bulblet.
The foregoing is illustrative of the best mode of the invention and details not described herein are within the common general knowledge of a person of ordinary skill in the art. The scope of the present invention is defined by the appended claims, and any equivalent modifications based on the technical teaching of the present invention are also within the scope of the present invention.
Claims (8)
1. A lily scale cuttage method capable of improving cuttage propagation coefficient and bulb size is characterized by comprising the following steps:
step 1, selecting healthy disease-free and insect-free lily bulbs with similar sizes, stripping the outermost scales and disinfecting the outermost scales;
step 2, making the outer layer scale in PP with 50-100 mg/L 333 Soaking in the solution for 10-20 min, and then dipping the base of the lily scales with PP of 50-100 mg/L 333 Clay prepared from the solution;
step 3, preparing a cuttage matrix;
and 4, cutting the outer-layer scales treated in the step 2 into a cutting medium in a manner of embedding pieces into the cutting medium.
2. The lily scale cuttage method for improving cuttage propagation coefficient and bulb size according to claim 1, wherein the PP in step 2 is 333 The concentration of the solution was 100mg/L.
3. The lily scale cuttage method for improving cuttage propagation coefficient and bulb size according to claim 1, wherein in the step 2, PP 333 In clay formulated in solution, PP 333 The volume ratio of the solution to the clay was 1.
4. The lily scale cutting method capable of improving the cutting propagation coefficient and the bulb size according to claim 1, wherein the disinfection method in the step 1 is as follows: soaking the scales in 50% carbendazim solution for 30-60 min, and then spreading the scales on a preservative film for drying in the shade for 24h.
5. The lily scale cutting method capable of improving the cutting propagation coefficient and the bulb size according to claim 1, wherein in the step 2, the specific method for dipping the outer scale after soaking is as follows: on a culture dish, prepared PP is used 333 The solution and the viscous red sandy soil subjected to early sterilization are mixed according to the volume ratio of 1:1 stirring into a slurry, and dipping a small amount of clay at a position of 0.5-1.0 cm away from the base of the scale.
6. The lily scale cuttage method capable of improving cuttage propagation coefficient and bulb size according to claim 1, wherein the step 3 specifically comprises: selecting the Pingshi peat soil and vermiculite according to the proportion of 1:1, then wetting the substrate by using a carbendazim solution until the substrate is in a state of being held to form a cluster and being immediately dispersed after being thrown off so as to achieve the effects of keeping the substrate wet and sterilizing the substrate.
7. The lily scale cutting method capable of improving the cutting propagation coefficient and the bulb size according to claim 1, wherein the step 4 specifically comprises: placing a substrate with the thickness of 5-7 cm on the bottom layer of the cutting bag, obliquely inserting the pretreated lily outer-layer scales into the substrate with the concave surfaces facing upwards uniformly, placing the scales and the substrate at intervals, and covering a layer of substrate with the thickness of 5-7 cm on the uppermost layer.
8. The method for lily bulb scale cutting with the improved cutting propagation coefficient and bulb size as claimed in claim 7, wherein in step 4, after the scale cutting is completed, the cut is punctured uniformly on the cutting bag every 2cm and multiple positions are placed in a dark room at 25 ℃ for cultivation.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090331A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Method for enhancing tulip tissue culture seedling |
| CN107466628A (en) * | 2017-08-02 | 2017-12-15 | 广西壮族自治区林业科学研究院 | A kind of method of the efficient cutting propagation of giant lily scale |
| CN107889630A (en) * | 2017-12-21 | 2018-04-10 | 安庆市枞阳县阳和苗圃 | A kind of scale cuttage method for culturing seedlings of lily |
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- 2022-10-28 CN CN202211339301.8A patent/CN115735584A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090331A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Method for enhancing tulip tissue culture seedling |
| CN107466628A (en) * | 2017-08-02 | 2017-12-15 | 广西壮族自治区林业科学研究院 | A kind of method of the efficient cutting propagation of giant lily scale |
| CN107889630A (en) * | 2017-12-21 | 2018-04-10 | 安庆市枞阳县阳和苗圃 | A kind of scale cuttage method for culturing seedlings of lily |
Non-Patent Citations (3)
| Title |
|---|
| 孙红梅;贾子坤;陆阳;陶文玲;王春夏;: "百合鳞片扦插繁殖的研究进展", 北方园艺, no. 02, 15 February 2009 (2009-02-15) * |
| 王爱勤, 周歧伟, 何龙飞, 许鸿源, 杨美纯: "百合试管结鳞茎的研究", 广西农业生物科学, no. 01, 30 March 1998 (1998-03-30), pages 71 - 75 * |
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