CN106342625B - Apple ring rot resistant cultivation method - Google Patents
Apple ring rot resistant cultivation method Download PDFInfo
- Publication number
- CN106342625B CN106342625B CN201610710393.4A CN201610710393A CN106342625B CN 106342625 B CN106342625 B CN 106342625B CN 201610710393 A CN201610710393 A CN 201610710393A CN 106342625 B CN106342625 B CN 106342625B
- Authority
- CN
- China
- Prior art keywords
- stock
- seedlings
- grafting
- transplanting
- cultivation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000012364 cultivation method Methods 0.000 title claims abstract description 14
- 230000012010 growth Effects 0.000 claims abstract description 18
- 238000005520 cutting process Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000003337 fertilizer Substances 0.000 claims abstract description 9
- 238000000338 in vitro Methods 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 4
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 11
- 238000011081 inoculation Methods 0.000 claims description 11
- 230000001965 increasing effect Effects 0.000 claims description 5
- 239000012883 rooting culture medium Substances 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 235000013877 carbamide Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000010455 vermiculite Substances 0.000 claims description 3
- 235000019354 vermiculite Nutrition 0.000 claims description 3
- 229910052902 vermiculite Inorganic materials 0.000 claims description 3
- 230000003044 adaptive effect Effects 0.000 claims 1
- 239000012499 inoculation medium Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 239000012882 rooting medium Substances 0.000 claims 1
- 201000010099 disease Diseases 0.000 description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 10
- 235000011430 Malus pumila Nutrition 0.000 description 10
- 235000015103 Malus silvestris Nutrition 0.000 description 10
- 241000220225 Malus Species 0.000 description 9
- 230000000694 effects Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 208000000260 Warts Diseases 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 201000010153 skin papilloma Diseases 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to an apple ring spot resistant cultivation method, which relates to the field of plant cultivation and comprises the following steps: (1) obtaining the virus-free rootstock: in 4-month middle ten days, cutting new tips of about 5cm from the stock in clear weather, performing in-vitro disinfection by adopting a mode of matching 75% alcohol by volume concentration and 0.1% mercury bichloride by mass concentration, establishing a high-efficiency rapid propagation system, domesticating and transplanting the obtained detoxified seedling to a seedbed after rooting in a test tube, and performing conventional fertilizer and water management to obtain the detoxified stock with healthy growth and consistent growth vigor; (2) grafting: in autumn of the year, the plant height of the stock is more than 70cm, and the stock can be used for grafting. By adopting the method, the ring spot of the apple can be effectively prevented and treated.
Description
Technical Field
The invention belongs to the field of plant cultivation, and particularly relates to an apple ring spot resistant cultivation method.
Background
The ring spot is one of the important diseases of apple branches and fruits. Often mixed with dry rot, anthracnose and the like, and is a great threat to fruit production and has a tendency of spreading and aggravating. The symptom is the disease of branches and trunks, dark brown, water stain or small ulcer spots are formed by taking skin holes as centers, and the skin holes are raised slightly to form wart and round shapes. The later dehydration is sunken, the edge is cracked and raised, and the shape of the flat circle is a grey color with the diameter of about 1 cm. A plurality of disease spots are dense, and the bark of a big branch of a trunk is rough, so the disease is called as 'rough bark disease', and the spots are sparse and have small black spots.
The existing method for preventing and treating apple ring spot mainly comprises the measures of eradicating an overwintering bacterium source, spraying pesticides and the like, but the method has the defects of poor prevention and treatment effect, environmental pollution, great harm to people and livestock and the like.
Disclosure of Invention
The invention aims to provide an apple ring spot resistant cultivation method, which can overcome the defects.
In order to achieve the above purpose, the invention provides the following technical scheme:
an apple cultivation method for resisting ring spot, comprising the following steps:
(1) obtaining the virus-free rootstock: in 4-month middle ten days, cutting new tips of about 5cm from the stock in clear weather, performing in-vitro disinfection by adopting a mode of matching 75% alcohol by volume concentration and 0.1% mercury bichloride by mass concentration, establishing a high-efficiency rapid propagation system, domesticating and transplanting the obtained detoxified seedling to a seedbed after rooting in a test tube, and performing conventional fertilizer and water management to obtain the detoxified stock with healthy growth and consistent growth vigor;
(2) grafting: in autumn of the year, the plant height of the stock is more than 70cm, and the method can be used for grafting and comprises the following steps:
1) preparing scions: selecting scion varieties, cutting robust annual branches by using a branch shear, removing leaves and topping, obliquely cutting downwards from about 1.5cm below scion buds to a depth of about 0.3cm of xylem, flatly cutting downwards from about 1.5cm above the buds with the xylem, intersecting with a transverse cut at the lower end, and taking off bud pieces;
2) preparation of rootstock: selecting a smooth part at a position 50cm away from the ground of the stock, carrying out downward longitudinal cutting with a xylem, wherein the size of a cut is equivalent to that of a bud sheet, removing a cut piece from the lower end in a beveling manner, embedding the bud sheet to align cambiums of the two, and tightly binding the bud sheet by using a telescopic film after grafting;
(3) planting the nursery stock: and in the spring of the second year, transplanting the grafted seedlings into a field.
The specific method of the rapid propagation system in the step (1) comprises the following steps: inoculating in-vitro sterilized young shoots to an inoculation culture medium on a super clean workbench, after 3d, selecting aseptic seedlings without browning and pollution and strong growth potential, transferring the aseptic seedlings to a fresh inoculation culture medium, subculturing once every 20d, obtaining enough stock tissue culture seedlings after 3 times of subculturing, detoxifying the tissue culture seedlings by adopting a mode of peeling growth points under a dissecting mirror, transferring the peeled growth points to the inoculation culture medium for subculturing, obtaining a large number of stock detoxified seedlings after 10 generations, detoxifying the stock seedlings which grow 20d secondarily, shearing stem tips of about 5cm, and placing the stem tips in a rooting culture medium for inducing rooting.
The inoculation culture medium comprises: MS +6-BA 0.5mg/L + NAA0.01 mg/L.
The rooting culture medium comprises: MS + NAA0.5 mg/L.
The domestication and transplanting to the seedbed comprises the following specific steps: and (4) transplanting the rooted seedlings to a 72-hole plug after rooting culture for 15 days, covering a film on the substrate with grass carbon, vermiculite and perlite in a volume ratio of 4: 2, and shading, and gradually increasing illumination and ventilation time after 20 days until the rooted seedlings are completely adapted to the external environment.
The fertilizer and water management specifically comprises the following steps: 1) planting: after 1-2 new leaves grow on the acclimatized and transplanted seedlings, flood-irrigating for one time, and after 2-3d, taking out the seedlings with soil balls from the hole trays, transplanting the seedlings to a field, wherein the seedling setting distance is 10-15cm, transplanting is carried out on a cloudy day or in the evening, and watering is carried out immediately after planting; 2) the water management is mainly performed by thoroughly drying and watering, and urea, monopotassium phosphate and ammonium sulfate are uniformly applied to the ground according to the mass ratio of 2:1, 20-25kg is applied to each mu, and the application is performed once per month.
The invention has the beneficial effects that:
1: the applicant finds that rich nutrient substances are provided for plants by a comprehensive cultivation method comprising obtaining detoxified stocks, rooting and domesticating, fertilizer and water management and the like, and meanwhile, the pollution of pathogenic bacteria is isolated from the source;
2: especially, the incidence rate of ring spot can be obviously reduced by changing the grafting height, after the grafting height is set to be 50cm, the incidence rate of the ring spot of the apple is only 3 percent and is far lower than that of other grafting heights, meanwhile, manpower and material resources are greatly saved, and the income of farmers is improved.
The application controls the height of grafting by selecting the disease-resistant rootstock, achieves the effect of controlling the ring spot of the apple trees, and unexpectedly discovers that the height of grafting is a key factor influencing the prevention and treatment effect, and the prevention and treatment effect can be influenced when the height is too low or too high.
Drawings
FIG. 1 shows the growth conditions of the seedlings with the grafting heights of 15cm and 50cm respectively.
Reference numerals: 1. 15cm of grafting point; 2. a 50cm grafted point; 3. the diseased area.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1
An apple cultivation method for resisting ring spot, comprising the following steps:
(1) obtaining the virus-free rootstock: in the middle ten days of 4 months, cutting new tips about 5cm from the 'green stock No. 1' stock in sunny weather, performing in-vitro disinfection by adopting a mode of using 75% alcohol by volume concentration and 0.1% mercury bichloride by mass concentration in a matching way, establishing a high-efficiency rapid propagation system, domesticating and transplanting the obtained detoxified seedlings to a seedbed after rooting in a test tube, and performing conventional fertilizer and water management to obtain detoxified stocks with healthy growth and consistent growth vigor;
(2) grafting: in autumn of the year, the plant height of the stock is more than 70cm, and the method can be used for grafting and comprises the following steps:
1) preparing scions: selecting a scion variety Fuji, cutting robust annual branches by using a branch shear, removing leaves and topping, obliquely cutting a cut from about 1.5cm below a scion bud downwards to reach about 0.3cm deep of xylem, flatly cutting the xylem from about 1.5cm above the bud downwards, intersecting with a transverse cut at the lower end, and taking off bud slices;
2) preparation of rootstock: selecting a smooth part at a position 50cm away from the ground of the stock, carrying out downward longitudinal cutting with a xylem, wherein the size of a cut is equivalent to that of a bud sheet, removing a cut piece from the lower end in a beveling manner, embedding the bud sheet to align cambiums of the two, and tightly binding the bud sheet by using a telescopic film after grafting;
(3) planting the nursery stock: and in the spring of the second year, transplanting the grafted seedlings into a field.
The specific method of the rapid propagation system in the step (1) comprises the following steps: inoculating in-vitro sterilized young shoots to an inoculation culture medium on a super clean workbench, after 3d, selecting aseptic seedlings without browning and pollution and strong growth potential, transferring the aseptic seedlings to a fresh inoculation culture medium, subculturing once every 20d, obtaining enough stock tissue culture seedlings after 3 times of subculturing, detoxifying the tissue culture seedlings by adopting a mode of peeling growth points under a dissecting mirror, transferring the peeled growth points to the inoculation culture medium for subculturing, obtaining a large number of stock detoxified seedlings after 10 generations, detoxifying the stock seedlings which grow 20d secondarily, shearing stem tips of about 5cm, and placing the stem tips in a rooting culture medium for inducing rooting.
The inoculation culture medium comprises: MS +6-BA 0.5mg/L + NAA0.01 mg/L.
The rooting culture medium comprises: MS + NAA0.5 mg/L.
The domestication and transplanting to the seedbed comprises the following specific steps: and (4) transplanting the rooted seedlings to a 72-hole plug after rooting culture for 15 days, covering a film on the substrate with grass carbon, vermiculite and perlite in a volume ratio of 4: 2, and shading, and gradually increasing illumination and ventilation time after 20 days until the rooted seedlings are completely adapted to the external environment.
The fertilizer and water management specifically comprises the following steps: 1) planting: after 1-2 new leaves grow on the acclimatized and transplanted seedlings, flood-irrigating for one time, and after 2-3d, taking out the seedlings with soil balls from the hole trays, transplanting the seedlings to a field, wherein the seedling setting distance is 10-15cm, transplanting is carried out on a cloudy day or in the evening, and watering is carried out immediately after planting; 2) the water management is mainly performed by thoroughly drying and watering, and urea, monopotassium phosphate and ammonium sulfate are uniformly applied to the ground according to the mass ratio of 2:1, 20-25kg is applied to each mu, and the application is performed once per month.
Comparative example 1
The height of the graft was 15cm, as in example 1.
Comparative example 2
The height of the graft was 30cm, as in example 1.
Comparative example 3
In autumn of the next year, the stock seedling is grafted two years later, the grafting height is 60cm, and the rest is the same as that of the example 1.
100 plants are planted at each grafting height, then the number of diseased plants is counted, and the disease incidence is calculated, and the result is shown in table 1.
TABLE 1 incidence of different grafting heights
The grafting height is 15cm | The grafting height is 30cm | The grafting height is 50cm | The grafting height is 60cm | |
Determining the number of plants | 100 | 100 | 100 | 100 |
Number of diseased plants | 85 | 56 | 3 | 13 |
Incidence (%) | 85% | 56% | 3% | 13% |
As shown in table 1, the incidence of the grafted height of 50cm was the lowest, only 3%, and was much lower than the incidence of the grafted height of 15cm and 30cm, and 50cm was the maximum height capable of grafting due to the limited height of plant growth within one year, so in order to test the incidence of the higher grafted height, the applicant conducted the test of 60cm grafted height in the second year, and the results showed that although the incidence of the disease was lower than the incidence of the grafted height of 15cm and 30cm, the incidence of the grafted height of 50cm was higher in the first year, and at the same time, if the height was increased, the nursery stock had to grow for one year, increasing the manpower and material resources for one year.
In addition, as shown in fig. 1, the plant with 1 being 15cm grafting point and 2 being 50cm grafting point has obvious ring spot disease symptom, i.e. a plurality of disease spots are dense, the trunk and big branch are formed and the bark is rough, i.e. the so-called 'rough bark disease', can be seen in the disease area 3 in fig. 1, but the plant with the grafting height of 50cm has no such symptom, therefore, the grafting height of 50cm is the most suitable cultivation method for reducing the ring spot disease of the apple branches.
Claims (5)
1. The cultivation method for the apple to resist the ring spot is characterized by comprising the following steps:
(1) obtaining the virus-free rootstock: in the middle ten days of 4 months, cutting 5cm of new tips on the 'green stock No. 1' stock in sunny weather, performing in-vitro disinfection by adopting a mode of using 75% alcohol by volume concentration and 0.1% mercury bichloride by mass concentration in a matching way, establishing a rapid propagation system, domesticating and transplanting the obtained detoxified seedlings to a seedbed after rooting in a test tube, and performing conventional fertilizer and water management to obtain the detoxified stocks with healthy growth and consistent growth vigor; the rapid propagation system comprises the following specific methods: inoculating in-vitro sterilized young shoots into an inoculation culture medium on a super clean workbench, after 3d, selecting aseptic seedlings without browning and pollution and strong growth potential to be transferred into a fresh inoculation culture medium, subculturing once every 20d, obtaining enough stock tissue culture seedlings after 3 times of subculture, detoxifying the tissue culture seedlings by adopting a mode of peeling growth points under a dissecting mirror, transferring the peeled growth points to the inoculation culture medium for subculture, obtaining a large number of stock detoxified seedlings after 10 generations, detoxifying the stock seedlings which grow 20d secondarily, cutting stem tips of 5cm, and placing in a rooting culture medium to induce rooting;
(2) grafting: in autumn of the year, the plant height of the stock is more than 70cm, and the method can be used for grafting and comprises the following steps:
1) preparing scions: selecting scion varieties, cutting robust annual branches by using a branch shear, removing leaves, topping, obliquely cutting downwards from about 1.5cm below scion buds to a depth of 0.3cm, flatly cutting downwards from about 1.5cm above the buds with xylem, intersecting with a transverse cut at the lower end, and taking off bud pieces;
2) preparation of rootstock: selecting a smooth part at a position 50cm away from the ground of the stock, carrying out downward longitudinal cutting with a xylem, wherein the size of a cut is equivalent to that of a bud sheet, removing a cut piece from the lower end in a beveling manner, embedding the bud sheet to align cambiums of the two, and tightly binding the bud sheet by using a telescopic film after grafting;
(3) planting the nursery stock: and in the spring of the second year, transplanting the grafted seedlings into a field.
2. The cultivation method according to claim 1, wherein the inoculation medium is: MS +6-BA 0.5mg/L + NAA0.01mg/L.
3. The cultivation method according to claim 1 or 2, wherein the rooting medium is: MS + NAA0.5mg/L.
4. The cultivation method according to claim 3, wherein the acclimatized and transplanted to the seedbed comprises the following specific steps: and (3) transplanting the rooted seedlings to a 72-hole plug after rooting culture for 15d, wherein the matrix is turf in volume ratio: vermiculite: perlite 4: 4: and 2, covering a film to shade, and gradually increasing the illumination and ventilation time after 20 days until the film is completely adaptive to the external environment.
5. The cultivation method according to claim 4, wherein the fertilizer and water management is specifically:
1) planting: after 1-2 new leaves grow on the acclimatized and transplanted seedlings, flood-irrigating for one time, and after 2-3d, taking out the seedlings with soil balls from the hole trays, transplanting the seedlings to a field, wherein the seedling setting distance is 10-15cm, transplanting is carried out on a cloudy day or in the evening, and watering is carried out immediately after planting;
2) the water management is mainly performed by thoroughly drying and watering, and urea, monopotassium phosphate and ammonium sulfate are uniformly applied to the ground according to the mass ratio of 2:1:1, 20-25kg of the fertilizer is applied to each mu once a month.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610710393.4A CN106342625B (en) | 2016-08-24 | 2016-08-24 | Apple ring rot resistant cultivation method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610710393.4A CN106342625B (en) | 2016-08-24 | 2016-08-24 | Apple ring rot resistant cultivation method |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106342625A CN106342625A (en) | 2017-01-25 |
CN106342625B true CN106342625B (en) | 2020-01-14 |
Family
ID=57844622
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610710393.4A Expired - Fee Related CN106342625B (en) | 2016-08-24 | 2016-08-24 | Apple ring rot resistant cultivation method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106342625B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106857254B (en) * | 2017-02-11 | 2019-04-26 | 青岛市农业科学研究院 | The efficient tissue culture and rapid propagation method of No. 1 elite stand of apple rootstock blueness anvil |
CN107568056A (en) * | 2017-08-16 | 2018-01-12 | 青岛市农业科学研究院 | A kind of method of quick breeding apple Early blooming and fruiting stock |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1050302A (en) * | 1990-01-09 | 1991-04-03 | 孙芳正 | The height of apple tree connects new varieties and young tree is worked as the annual bearing method |
CN101156606B (en) * | 2007-11-20 | 2010-10-20 | 史建生 | A plants antiseptic as well as preparing method and application |
CN101347087A (en) * | 2008-09-04 | 2009-01-21 | 山东农业大学 | Method for enhancing disease resistance of fruit tree using interstock |
CN102187799A (en) * | 2011-04-12 | 2011-09-21 | 天津力臣阳光果品生产力促进有限责任公司 | Method for preventing and controlling apple tree branch ring spots |
-
2016
- 2016-08-24 CN CN201610710393.4A patent/CN106342625B/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN106342625A (en) | 2017-01-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107926423B (en) | Production method of kiwifruit canker-resistant bidirectional detoxification big seedlings | |
CN104642109B (en) | A kind of is the method that outer implant builds Bulbus Allii micro-propagation with bulb sheet | |
CN112753473A (en) | Method for cutting and breeding tea seedlings by inoculating mixed arbuscular mycorrhizal fungi | |
CN109845500B (en) | Method for raising seedlings of populus tomentosa by cutting tender branches | |
CN105265274A (en) | Flowering peach grafting and seedling method | |
Waman et al. | Optimization of factors affecting in vitro establishment, ex vitro rooting and hardening for commercial scale multiplication of silk banana (Musa AAB) | |
CN110946064A (en) | High-survival-rate transplanting method for moss associated dendrobium officinale limestone surface | |
Chipojola et al. | Effect of tree age, scion source and grafting period on the grafting success of cashew nut (Anacardium occidentale L.) | |
CN106538251A (en) | It is a kind of reduce large leaf Camellia sinensis wither bud disease fast seedling-cultivating method | |
CN106342625B (en) | Apple ring rot resistant cultivation method | |
Choudhary et al. | In vitro propagation of two commercially important bamboo species (Bambusa tulda Roxb. and Dendrocalamus stocksii Munro.) | |
CN102771304A (en) | Method for cuttage seedling of buerretiodendron hsienmu at all seasons | |
CN112219579B (en) | Construction method and application of castanopsis hystrix juvenile cutting orchard | |
Souza et al. | Production of pear trees grafted under hydroponic conditions. | |
Wright et al. | Control of bacterial soft rot of calla (Zantedeschia spp.) by pathogen exclusion, elimination and removal | |
CN106342555B (en) | Method for breeding new species of natural hetero-alternating heterogeneous populus euphratica and populus griseus | |
CN105875313A (en) | Planting method for Prunus salicina L | |
CN117243001A (en) | Seedling raising method for gramineous plants | |
CN109526743B (en) | Rapid propagation and fine variety breeding method for garlic callus | |
CN111492978A (en) | Tissue culture rapid propagation method of Eucalyptus macrocarpa 1212 variety | |
CN109496706A (en) | A kind of sterile brachyplast of Mao Xu Cao quickly breed before explant preprocess method | |
CN115176608A (en) | Rapid seedling method for dendrobium officinale stem node induced buds | |
CN103734021A (en) | Collection and induction method for alnus nepalensis axillary bud explants | |
CN111201934B (en) | Intermediate stock rooting cultivation method for avocados | |
CN107360756B (en) | Method for promoting rapid and multiple germination of golden plum seeds into seedlings |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200114 |