CN101396446A - Method for increasing the parasitism performance of parasitic wasp - Google Patents
Method for increasing the parasitism performance of parasitic wasp Download PDFInfo
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- CN101396446A CN101396446A CNA2008101561629A CN200810156162A CN101396446A CN 101396446 A CN101396446 A CN 101396446A CN A2008101561629 A CNA2008101561629 A CN A2008101561629A CN 200810156162 A CN200810156162 A CN 200810156162A CN 101396446 A CN101396446 A CN 101396446A
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- ethanol extract
- pest control
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- 230000003071 parasitic effect Effects 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 11
- 230000024241 parasitism Effects 0.000 title claims description 7
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 19
- 241001092084 Agrimonia Species 0.000 claims abstract description 18
- 235000016993 Agrimonia Nutrition 0.000 claims abstract description 18
- 239000000469 ethanolic extract Substances 0.000 claims abstract description 18
- 241000256247 Spodoptera exigua Species 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 6
- 241000256844 Apis mellifera Species 0.000 claims description 23
- 241000238631 Hexapoda Species 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 6
- 244000045947 parasite Species 0.000 claims description 3
- 239000003814 drug Substances 0.000 abstract description 9
- 241001481304 Vespoidea Species 0.000 abstract description 5
- 238000012545 processing Methods 0.000 abstract description 4
- 241000500110 Meteorus pulchricornis Species 0.000 abstract 3
- 235000013311 vegetables Nutrition 0.000 abstract 1
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000000419 plant extract Substances 0.000 description 3
- 241000256836 Apis Species 0.000 description 2
- 235000016068 Berberis vulgaris Nutrition 0.000 description 2
- 241000335053 Beta vulgaris Species 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 238000002481 ethanol extraction Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000007952 growth promoter Substances 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 244000307697 Agrimonia eupatoria Species 0.000 description 1
- 241001278836 Agrimonia pilosa Species 0.000 description 1
- 235000000641 Agrimonia pilosa Nutrition 0.000 description 1
- 241000960374 Aphidiinae Species 0.000 description 1
- 241000317929 Chalcididae Species 0.000 description 1
- 241000123860 Christella parasitica Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241000721703 Lymantria dispar Species 0.000 description 1
- 241000500111 Meteorus Species 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 241000985245 Spodoptera litura Species 0.000 description 1
- 241000130764 Tinea Species 0.000 description 1
- 208000002474 Tinea Diseases 0.000 description 1
- 241000256618 Trichogramma Species 0.000 description 1
- 230000000146 antalgic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 235000000125 common agrimony Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
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- 239000000575 pesticide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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Abstract
The invention discloses a method for improving parasitic ability of parasitic wasps by utilizing a hairyvein agrimonia herb and bud ethanol extract. The method is designed against the situation that the parasitic ability of the parasitic wasps in the natural world is usually not high, when in great occurrence amount of pests, the natural pest control effect is small, and the complete control of the pests can not be realized. The use of the traditional Chinese medicine hairyvein agrimonia herb and bud ethanol extract for processing meteorus pulchricornis can significantly improve the parasitic amount of the meteorus pulchricornis to vegetable major pest spodoptera exigua and enhance the pest control ability of the parasitic wasps. Compared with the traditional pest control which takes the pests as a target object, the technology takes the parasitic wasps which are the parasitic natural enemies of the pests as the target object and uses the hairyvein agrimonia herb and bud ethanol extract to improve the parasitic amount and the parasitic speed of the meteorus pulchricornis, so as to improve the pest control effect. The technology not only has evident synergy, but also has high safety and can effectively improve the biological control level of the pests. The economic, the social and the ecological benefits are very significant after a large number of applications on the production.
Description
One, technical field
The invention belongs to the biological control of insect pests technical field, be specifically related to a kind of method of utilizing the Chinese medicine Herba Agrimoniae extract to improve the outstanding cocoon honeybee parasitic ability of macle.
Two, technical background
Parasitic wasp is the parasite of insecticide, and its kind is abundant, reaches 50,000 kinds more than.Because of parasitic wasp has advantages such as pollution-free, that specialization is strong controlling naturally of insect, form important component part into biological control of insect pests.Though yet the parasitic wasp kind is numerous, can the kind that plays a significant role aborning seldom, only several parasitic wasps such as trichogramma have obtained successful Application.A kind of parasitic wasp is wanted and can play a significant role on Pest Control, is the factor of most critical to the high parasitic rate of target pest.Both at home and abroad the biological characteristics, extraneous factor that then concentrates on parasitic wasp itself mostly about the research of parasitic wasp be to aspects such as the parasitism performance of parasitic wasp destruction evaluations, and with regard to how initiatively to promote the parasitism performance of parasitic wasp by effective measures research report is not arranged.
China's floristics is abundant equally, many plant extracts have showed tangible insecticidal activity, therefore also had about the plant source extract the dysgenic research of parasitic wasp, reported that plant extracts such as Cyclosorus parasiticus CL. Forwell-L., Fructus Momordicae charantiae, Herba Eupatorii Odorati do not have harmful effect to eclosion rate and the parasitic rate of the glaze Ji of Thebes chalcid fly.But there is not plant extract parasitic wasp to be had the research report of beneficial effect as yet.It is a kind of insecticide endoparasitism honeybee that macle hangs cocoon honeybee (Meteorus pulchriconis) (Hymenoptera, Aphidiidae Braconidae), belong to the capacitive parasitic wasp, its host range is wider, can parasitize the larva of many Lepidoptera nocturnal moth class insects such as beet armyworm, Prodenia litura, bollworm, gypsymoth.Herba Agrimoniae Agrimonia pilosa Ledeb. is a kind of Rosaceae herbaceos perennial, in the most of area of China distribution is arranged all, and as a kind of Chinese medicine, it has effects such as antitumor, anti-inflammatory and antalgic, blood sugar lowering, blood pressure lowering and enhancing human body immunity function.This paper studies the influence of hairyvein agrimonia herb and bud ethanol extract to the outstanding cocoon honeybee parasitism of macle first, and result of study will have great importance to the level that lifting utilizes parasitic wasp to carry out great biological control of insect pests.
Three, summary of the invention
1, goal of the invention
A kind of method of utilizing the Chinese medicine hairyvein agrimonia herb and bud ethanol extract to improve the outstanding cocoon honeybee parasitic capacity of macle is provided, can improves the control effect of the outstanding cocoon honeybee of macle safely and effectively, promote the Biological control level of insect insect.
2, technical scheme
For achieving the above object, the present invention selects for use the ethanol extraction of Chinese medicine Herba Agrimoniae to handle the outstanding cocoon honeybee of macle, has obtained a kind of biological control method safely and efficiently, and its application has significantly improved parasitic ability and the parasitic speed of parasitic wasp.
The present invention is the parasitic increased functionality method of the outstanding cocoon honeybee of a kind of macle, it is characterized in that utilizing hairyvein agrimonia herb and bud ethanol extract to handle the outstanding cocoon honeybee of macle, improves parasitic amount and the parasitic speed of parasitic wasp to beet armyworm.
3, beneficial effect
Method of the present invention compared with prior art, produce following beneficial effect: (1) breaks through traditional Pest control is the target object with the insect, with the outstanding cocoon honeybee of insect parasite macle is the target object, improves the pest control effect of parasitic wasp, has improved the biological control of insect pests level conscientiously; (2) utilize Chinese medicine to substitute middle high toxicity pesticide and be applied to the farmland, effectively reduced environmental pollution.
The specific embodiment:
Embodiments of the invention are the methods that adopt indoor bioassay.By indoor bioassay, clear and definite hairyvein agrimonia herb and bud ethanol extract is to the potentiation of the parasitic function of the outstanding cocoon honeybee of macle.
For trying material: Herba Agrimoniae is buied by pharmacy of Tongrentang;
Outstanding cocoon honeybee of macle and beet armyworm are indoor feeding, and wherein beet armyworm is that man-made feeds are raised, and the outstanding cocoon honeybee of macle is that beet armyworm goes up raising.
Test method:
The extraction of hairyvein agrimonia herb and bud ethanol extract
With Universalpulverizer Chinese medicine Herba Agrimoniae and Cortex Meliae are pulverized, respectively taken by weighing 200g dry powder and put in the people 1000mL triangular flask, add 75% ethanol 500ml, lixiviate at room temperature 14 days is filtered.With Rotary Evaporators concentrating under reduced pressure in water-bath, steam ethanol, Chinese medicine extract and dry, after be dissolved in the 25ml acetone.The original concentration of Chinese medicine ethanol extraction is represented as follows: concentration=medical material dry weight/final volume.Calculate by this, the original concentration of Herba Agrimoniae is 8g/ml.Extracting solution is stored in 4 ℃ of refrigerators standby.
Hairyvein agrimonia herb and bud ethanol extract is to the influence of the outstanding cocoon honeybee parasitism of macle
Measure 1ml and be dissolved in the acetone hairyvein agrimonia herb and bud ethanol extract, be diluted in 20ml 10% hydromel, as the extra-nutrition of parasitic wasp, simultaneously with the 1ml acetone diluted in 20ml 10% hydromel in contrast.Hydromel is added on the filter paper bar, add 0.2ml on each filter paper bar, the filter paper bar hangs in the disposable cup, put into a parasitic wasp for every glass, add 50 beet armyworm 3 instar larvaes simultaneously, every 2d changes not parasitic beet exigua larvae, and the beet armyworm that swaps out is divided to 6 orifice plates, statistics parasitic wasp cocoon number every day, 30 of every processing (cup).Also establish in addition and do not put into parasitic wasp, only put the filter paper bar that contains above-mentioned same concentrations hairyvein agrimonia herb and bud ethanol extract hydromel and the processing of beet armyworm, observe growth promoter and survival condition that Herba Agrimoniae extract is handled the back beet armyworm, totally 10 glasss.
Implementation result
Hairyvein agrimonia herb and bud ethanol extract is to the influence of the outstanding cocoon honeybee parasitic ability of macle
Studied the influence (Fig. 1) of hairyvein agrimonia herb and bud ethanol extract to the outstanding cocoon bee imago of macle, result of study shows, after the outstanding cocoon honeybee of macle is got food and contains the Apis water of 0.38g/ml hairyvein agrimonia herb and bud ethanol extract, its parasitic ability to beet armyworm significantly strengthens, every outstanding cocoon honeybee of macle on average can produce 28.7 honeybee offsprings, and (it causes death beet exigua larvae, and cocoon or the Tinea Apis output arranged), be significantly higher than matched group (20.6 honeybee offsprings).Simultaneously single observation with Herba Agrimoniae processing beet armyworm is shown that Herba Agrimoniae does not exert an influence to growth promoter and the survival of beet armyworm.
Fig. 1 hairyvein agrimonia herb and bud ethanol extract is to the influence of the parasitic speed of the outstanding cocoon honeybee of macle
Hairyvein agrimonia herb and bud ethanol extract has also been accelerated the parasitic speed of parasitic wasp. From parasitic amount every day (Fig. 2), hairyvein agrimony Processed group arrived parasitic peak on the 4th day after emergence, every parasitic wasp parasitic amount reaches 10.8, and control group is then the 6th It arrives parasitic peak, and every parasitic wasp parasitic amount is 6.7.
Fig. 2 hairyvein agrimonia herb and bud ethanol extract is to the impact of the parasitic speed of the outstanding cocoon honeybee of macle.
Claims (2)
1, a kind of is the insect pest control method of target object with the insect parasite, it is characterized in that utilizing Herba Agrimoniae extract to handle parasitic wasp, improves the parasitic ability of parasitic wasp to insect.
2, Herba Agrimoniae extract according to claim 1 is characterized in that utilizing hairyvein agrimonia herb and bud ethanol extract to improve parasitic amount and the parasitic speed of the outstanding cocoon honeybee of macle to beet armyworm to the raising effect of the parasitism performance of parasitic wasp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101561629A CN101396446B (en) | 2008-10-06 | 2008-10-06 | Method for increasing the parasitism performance of parasitic wasp |
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| CN2008101561629A CN101396446B (en) | 2008-10-06 | 2008-10-06 | Method for increasing the parasitism performance of parasitic wasp |
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| Publication Number | Publication Date |
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| CN101396446A true CN101396446A (en) | 2009-04-01 |
| CN101396446B CN101396446B (en) | 2011-01-26 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340946A (en) * | 2013-07-12 | 2013-10-09 | 江苏省农业科学院 | Method for improving tolerance of parasitic wasp by botanical extract |
| CN108522435A (en) * | 2018-06-08 | 2018-09-14 | 河南农业大学 | A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp |
| US10813361B2 (en) | 2017-05-24 | 2020-10-27 | Jiangsu Academy Of Agricultural Sciences | Use of agrimonia pilosa extract in preparation of drug for removing plant virus in tomato plants |
| CN112913785A (en) * | 2021-01-29 | 2021-06-08 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
| CN113040097A (en) * | 2021-03-15 | 2021-06-29 | 江苏科技大学 | Parasitic wasp artificial feeding method taking mulberry borer as host |
| CN113796356A (en) * | 2021-09-06 | 2021-12-17 | 广东省科学院动物研究所 | Method for detecting and evaluating quality of Cereus lucidus imagoes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044766C (en) * | 1993-01-01 | 1999-08-25 | 李发泰 | Chinese herbal pesticide without envirenmental pollution and preparation thereof |
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2008
- 2008-10-06 CN CN2008101561629A patent/CN101396446B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340946A (en) * | 2013-07-12 | 2013-10-09 | 江苏省农业科学院 | Method for improving tolerance of parasitic wasp by botanical extract |
| US10813361B2 (en) | 2017-05-24 | 2020-10-27 | Jiangsu Academy Of Agricultural Sciences | Use of agrimonia pilosa extract in preparation of drug for removing plant virus in tomato plants |
| CN108522435A (en) * | 2018-06-08 | 2018-09-14 | 河南农业大学 | A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp |
| CN112913785A (en) * | 2021-01-29 | 2021-06-08 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
| CN112913785B (en) * | 2021-01-29 | 2022-08-02 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
| CN113040097A (en) * | 2021-03-15 | 2021-06-29 | 江苏科技大学 | Parasitic wasp artificial feeding method taking mulberry borer as host |
| CN113796356A (en) * | 2021-09-06 | 2021-12-17 | 广东省科学院动物研究所 | Method for detecting and evaluating quality of Cereus lucidus imagoes |
| CN113796356B (en) * | 2021-09-06 | 2023-03-14 | 广东省科学院动物研究所 | Method for detecting and evaluating quality of Cereus lucidus imagoes |
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| CN101396446B (en) | 2011-01-26 |
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