CN106342905A - Agricultural insecticide composition containing phryma leptostachya extractive - Google Patents
Agricultural insecticide composition containing phryma leptostachya extractive Download PDFInfo
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- CN106342905A CN106342905A CN201610742930.3A CN201610742930A CN106342905A CN 106342905 A CN106342905 A CN 106342905A CN 201610742930 A CN201610742930 A CN 201610742930A CN 106342905 A CN106342905 A CN 106342905A
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- herba speranskiae
- speranskiae tuberculatae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/88—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with three ring hetero atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
- A01N47/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom containing —O—CO—O— groups; Thio analogues thereof
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- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention discloses an agricultural insecticide composition containing phryma leptostachya extractive, comprising a first active component and a second active component, wherein the mass ratio of the first active component to the second active component is 15:1-1: 5; the first active component is phryma leptostachya extractive, and the second active component is one of tolfenpyrad, methylamino abamectin, imidacloprid, chlorfenapyr, spirotetramat, buprofezin insecticides. The agricultural insecticide composition of the invention can be prepared to be suspending agent, emulsion in water and microemulsion allowed to use agriculturally, and has good preventive effects to brassicaceous vegetable plutella xylostella, cabbage caterpillar, apple scale insect, apple aphid, and cucumber aphid. The agricultural insecticide composition effectively solves the problem of not ideal prevention effect of phryma leptostachya extractive insecticide, reduces dosage of tolfenpyrad, methylamino abamectin, imidacloprid, chlorfenapyr, spirotetramat, buprofezin insecticides, and lighten environment pressure; the compound of the two components have obvious synergism and good crops safety.
Description
Technical field
The invention belongs to technical field of compounded pesticide, it is related to a kind of agricultural insecticidal composite and in particular to a kind of contain thoroughly
The agricultural insecticidal composite of GUCAO extract.The present invention gives the preparation formulation of this Pesticidal combination and agriculturally
Application.
Background technology
With the enhancing of people's environmental protection, social sustainable development consciousness, minimum is down in the side effect of pesticide, exploitation is biological
Rationally pesticide has become global common recognition.Botanical pesticide has low toxicity, low-residual, substantially harmless to non-target organism, right
The advantages of environmental nonpollution, development and appliable plant source pesticide have been increasingly becoming a kind of trend.And with the public to green food
Product, pollution-free food demand increasing, also grow with each passing day to the demand of botanical pesticide in market.Development plant source agriculture
Medicine becomes the inexorable trend of society and scientific development.
Herba speranskiae tuberculatae (phryma leptostachya l.) belongs to herbaceos perennial for Phrymaceae Herba speranskiae tuberculatae, also known as always
Woman's needlework, malicious fly grass, american lopseed herb or root, a Ma Guang etc..Herba speranskiae tuberculatae is general to be distributed in the Himalaya, North America and east Asia,
It has been used as traditional pesticide plant in East Asia.It is each that Herba speranskiae tuberculatae is mainly distributed on northeast, North China and the Yangtze river basin in China
Ground.According to records, Herba speranskiae tuberculatae herb and root are poisonous, hyoscine, and nature and flavor are puckery；Energy heat clearing away, controls impetigo, scabies and worm sore；Root can
Kill fly larvae, herb can kill vegetable Pieris rapae.In recent years, Inst. of Pesticides, Xibei Univ. of Agricultural ＆. Forestry Science ＆. Technolo is to Herba speranskiae tuberculatae system
System research, has developed Herba speranskiae tuberculatae microemulsion formulation, has had good preventive effect to Pieris rapae, oriental armyworm, diamondback moth etc..
Tolfenpyrad, Affirm (Merck Co.), imidacloprid, capillary, spiral shell worm ethyl ester, Buprofezin are to develop in recent years
Some efficient pesticides, its mechanism of action is more unique, crop various pests are had efficiently, the effect of wide spectrum, and be poison
Relatively low, the environmentally safe insecticide variety of property, becomes presently preferred insecticide.
The physicochemical property of above-mentioned several pesticides, insecticidal mechanism and in application agriculturally are briefly described below.
Tolfenpyrad, other titles: it is bright to catch worm, English common name: tolfenpyrad, chemical name: n- [4- (4- methyl
Phenoxy group) benzyl] the chloro- 5- pyrazolecarboxamide of -1- methyl -3- ethyl -4-.Tolfenpyrad is developed for Mitsubishi chemical company
Novel pyrazole heterocyclic Insecticidal and acaricidal agent, its mechanism of action is to hinder the electronics in mitochondrial metabolic system to pass on system multiple
Fit i, so that electronics reception and registration is hindered, makes insecticide not be provided that and storing energy, is referred to as Mitochondrial electron and passes on again
Fit Inhibitorses.Insecticidal spectrum is wide, has action of contace poison.There is specially good effect to lepidoptera pest diamondback moth, Thysanoptera thrips.This medicine
There is preferable quick-acting, the lasting period is longer, up to 10 days about.It is widely used in the crops such as preventing and treating vegetable, fruit tree, flowers, Folium Camelliae sinensis
On insect,
Affirm (Merck Co.), also known as emaricin (because of spit of fland of going out), English common name: emamectin.Methylamino Avermectin
Element is the efficient insecticide of a kind of new semi-artificial synthesis from fermented product avilamycin b1 synthesis, acaricide.Methylamino AVM hereinafter
The Action mechanism of rhzomorph is to hinder nerve conduction, lead to insect paralysis it is impossible to normal activity and lethal die.Insecticide is acted on
Mode is mainly stomach poison function, and contact efficacy is slow, need 3～4d about just can make insect death, be mainly used in preventing and treating cross
Flower section vegetable diamondback moth, Pieris rapae, the insect such as beet armyworm and oriental tobacco budworm.
Imidacloprid, English common name: imidacloprid, chemical name: 1- (6- chloropyridine -3- pyridylmethyl)-n-
Nitroimidazoline -2- base amine.Imidacloprid is nitro-methylene-type systemic insecticide, is the effect of nAChR
Body, interference pest movements nervous system makes its chemical signal transmit failure, have tag, stomach toxicity and the multiple drug effect of interior suction.Imidacloprid
Have wide spectrum, efficiently, low toxicity, low-residual, insect is not likely to produce resistance, the features such as to people, animal, plant and natural enemies security, mainly
For preventing and treating sucking pest, such as aphid, leafhopper, thrips, trialeurodes vaporariorum and colorado potato bug and frit fly etc..
Capillary, also known as chlorfenapyr, English common name: chlorfenapyr, chemical name: bromo- 2- (4- chlorphenyl) -1-
Ethoxyl methyl -5- trifluoromethyl pyrpole -3- nitrile；The bromo- 2- of 4- (4- chlorphenyl) -1- ethoxyl methyl -5- trifluoromethyl pyrpole -
3- nitrile.Capillary is new pyrrole class compound, acts on the mitochondrion of insecticide cells in vivo, by the many work(in insecticide body
Can oxidase work, mainly suppression adenosine diphosphate (ADP) (adp) is to the conversion of adenosine triphosphate (atp).Capillary has stomach toxicity
And action of contace poison.Strong in blade face permeability, there is certain systemic action, and have that insecticidal spectrum is wide, preventive effect is high, holding effect length, safety
Feature.Capillary is mainly used in preventing and treating brassicaceous vegetable diamondback moth, Pieris rapae, the insect such as beet armyworm.
Spiral shell worm ethyl ester, English common name: spirotetramat, chemical name: cis -3- (2,5- 3,5-dimethylphenyl) -8-
Methoxyl group -2- oxo -1- azaspiro [4.5] decyl- 3- alkene -4- base ethyl carbonate, cis -4- (ethyl oxy carbonyl) -8- methoxyl group -
3- (2,5- xylyl) -1- azaspiro [4.5] decyl- 3- alkene -2- ketone.Spiral shell worm ethyl ester is the season of Bayer Cropscience Co., Ltd's exploitation
Mek-Tol Unit two-way Uptake and translocation insecticide, can effectively prevent and treat various suckings pest, such as aphid, thrips, wood louse, aleyrodid
With scale insect etc..Lasting period is up to 8 weeks, and applicable chief crop includes Cotton Gossypii, Semen sojae atricolor, Citrus, tropical fruit tree, nut, Portugal
Grape, Flos lupuli (Flos Humuli Lupuli), Rhizoma Solani tuber osi and vegetable etc., and to important beneficial insect such as ladybug, wasp fly and parasitic wasp, there is good selectivity.
Buprofezin, also known as Buprofezin, buprofezin.English common name: buprofezin, chemical name: 2- tertiary butyl imido
Base -3- isopropyl -5- phenyl -3,4,5,6- tetrahydrochysene -2h-1,3,5- thiadiazine -4- ketone.Buprofezin is to be opened by Japanese pesticide company
The diazthiness insect growth regulator, IGR sent out.By suppressing chitin synthesis and interference metabolism, insect is made can not normally to slough off
Skin and abnormal and gradually dead.Active height, selectivity are good, the feature of longevity of residure length.There is specially good effect to plant hopper, leafhopper, aleyrodid,
Also there is preferable prevention effect to some scale insects such as arrowhead scales, long white a red-spotted lizards.
The new type disinsection such as Tolfenpyrad, Affirm (Merck Co.), imidacloprid, capillary, spiral shell worm ethyl ester, Buprofezin in recent years
Quickly, market sales volume increases the development speed of agent year by year.Due to its preventive effect preferably, peasant is used for multiple times in a large number, leads to many evils
Worm gradually produces resistance to it, causes dosage to continue to increase, and persticide residue increases, and non-target organism and environment are also produced
Give birth to harmful effect.
Content of the invention
The technical problem to be solved in the present invention: as the Herba speranskiae tuberculatae extract of botanical pesticide, some crop pests are had
There is certain cytotoxicity.But because Herba speranskiae tuberculatae extract is relatively low to the active component content of crop pest, quick-acting is poor, right
The effect of controlling of crop pest is typically not so good as chemical pesticide.Above-mentioned chemical pesticide Tolfenpyrad, Affirm (Merck Co.), imidacloprid,
Capillary, spiral shell worm ethyl ester, Buprofezin have the relatively new mechanism of action and excellent prevention effect, but various agricultural insect is right
Plurality of kind creates Drug resistance.Chemical pesticide using dosage is reduced by rational mixture, to crops No-harmful apple orchard
Significant；Also expanded the application of botanical pesticide simultaneously, explored what botanical pesticide-chemical pesticide mixture used
New model.
Solve the scheme of technical problem: a person of good sense passes through substantial amounts of test for a long time and finds, by Herba speranskiae tuberculatae extract and effect machine
In the different novel pesticide Tolfenpyrads of reason, Affirm (Merck Co.), imidacloprid, capillary, spiral shell worm ethyl ester, Buprofezin one
Plant mixture to use, have significant synergies, can significantly improve and Pieris rapae, diamondback moth, scale insect, aphid, aleyrodid are prevented
Control effect, reduce dosage, expanding prevention is composed, reduce cost accounting, resistance problem can be solved, increase the service life, improve
Safety to crop.
The present invention using Herba speranskiae tuberculatae extract as the first active component, chemical pesticide Tolfenpyrad, Affirm (Merck Co.),
One of imidacloprid, capillary, spiral shell worm ethyl ester, Buprofezin as the second active component, the first active component and second activity
Composition binary built forms the agricultural insecticidal composite with compound synergic.
Specifically, the technical scheme is that a kind of agricultural insecticidal composite containing Herba speranskiae tuberculatae extract, its bag
Include the first active component Herba speranskiae tuberculatae extract, and the second active component Tolfenpyrad, Affirm (Merck Co.), imidacloprid, worm
One of demodicid mite nitrile, spiral shell worm ethyl ester, Buprofezin, the mass ratio of the first active component and the second active component is 15:1～1:5.
Herba speranskiae tuberculatae extract of the present invention, can be prepared by following methods:
Herba speranskiae tuberculatae after being pulverized with organic solvent soaking at room temperature, supersound extraction, filters, obtains final product deep after sloughing organic solvent
Careless extract.
Preferably, organic solvent here, such as can adopt methanol, ethanol, acetone, ethyl acetate, dichloromethane, chlorine
Imitate or its mixed solvent.For situation, the component as mixed solvent and each component of extracting Herba speranskiae tuberculatae using mixed solvent
Between ratio, the present invention particularly do not limit.
Further preferred preparation method as Herba speranskiae tuberculatae extract of the present invention:
After the Herba speranskiae tuberculatae being dried is pulverized, with methanol soaking at room temperature 24h；Adopt supersound extraction 3 times, each supersound extraction again
30min, filters；Repeat to extract 3 times, merging filtrate, concentrating under reduced pressure obtains Herba speranskiae tuberculatae extract.
Agricultural insecticidal composite containing Herba speranskiae tuberculatae extract of the present invention, the dosage form of its pesticidal preparations is suspending agent,
Aqueous emulsion or microemulsion.
The present invention also provides the described agricultural insecticidal composite containing Herba speranskiae tuberculatae extract further in preventing and treating crops worm
Purposes in evil.
This agricultural insecticidal composite can be used for preventing and treating multiple insect pests of multiple kinds of crops, and for example, brassicaceous vegetable is little
Diamond-back moth, Pieris rapae, Fructus Mali pumilae scale insect, apple aphid, Cucumber blight.
Compared with prior art, what the present invention produced has the beneficial effect that following aspect:
(1) Herba speranskiae tuberculatae extract is as a kind of new plant insecticide, and it is to the preventing and treating of crop pest with stomach function regulating of tagging
Based on toxic action, but due in extract active component content relatively low, lead to its to preventing and treating target preventive effect poor.Azoles worm acyl
This several pesticides mechanism of action of amine, Affirm (Merck Co.), imidacloprid, capillary, spiral shell worm ethyl ester, Buprofezin is more special,
To preventing and treating target effect is significant, but chronic administration is easy to produce resistance.
The present invention is by Herba speranskiae tuberculatae extracts different for the mechanism of action and Tolfenpyrad, Affirm (Merck Co.), imidacloprid, worm
One of demodicid mite nitrile, spiral shell worm ethyl ester, Buprofezin carry out binary built, form new botanical pesticide-chemical pesticide composite class
The agricultural insecticidal composite of type.The activity of this Pesticidal combination and insecticidal effect are not the simple superposition of each composition activity, and
It is to have significant potentiation, has both overcome the poor shortcoming of botanical pesticide preventive effect, reduce the use agent of chemical pesticide again
Amount.Additionally, the advantage of described agricultural insecticidal composite is also manifested by component rationally, good disinsection effect, improve crop safety,
The generation of delaying drug resistance, and adapted to the requirement of current pesticide reduction potentiation, improve the quality of agricultural product.
(2) present invention expands preventing and treating spectrum, primary pest such as brassicaceous vegetable diamondback moth, the dark greyish green to multiple kinds of crops
Worm, apple yellow aphid, Cucumber blight, Fructus Mali pumilae scale insect have good prevention effect.
(3) present invention is prepared for the preparation of different dosage form, learns through safety evaluatio, and each preparation is good to crop safety,
Meet the security requirement of pesticidal preparations.
Specific embodiment
In order that the purpose of the present invention, technical scheme and beneficial effect become more apparent, with reference to embodiments, to this
Invention is further elaborated.
(1) preparation of Herba speranskiae tuberculatae extract
Herba speranskiae tuberculatae extract described in the present embodiment can conventionally be prepared, for example Soakage extraction method, ultrasonic vibration
Extraction method etc..In order to fully prepare described Herba speranskiae tuberculatae extract, improve the yield of Herba speranskiae tuberculatae extract, shorten preparation described simultaneously
The time of Herba speranskiae tuberculatae extract.The present embodiment preferably employs Soakage extraction and extracts, with ultrasonic vibration, the method combining, and preparation is thoroughly
GUCAO extract.
Herba speranskiae tuberculatae preferably selects dried Herba speranskiae tuberculatae, uses organic solvent Soakage extraction after pulverizing.The present embodiment is available for selecting
Organic solvent has multiple, such as methanol, ethanol, acetone, ethyl acetate, dichloromethane, chloroform or its mixed solvent.
As the method for optimizing of Herba speranskiae tuberculatae extract preparation, particularly as follows: after weighing Herba speranskiae tuberculatae pulverizing, methanol soaking at room temperature
After 24h, using supersound extraction three times (30min/ time), repeat to extract three times.Filter, merging filtrate, concentrating under reduced pressure obtains Herba speranskiae tuberculatae
Extract, yield is 4.2-5.6%.
(2) preparation preparation part
Agricultural insecticidal composite of the present invention, first group is divided into the Herba speranskiae tuberculatae extract that can prepare by said method,
Second component selects one of Tolfenpyrad, Affirm (Merck Co.), imidacloprid, capillary, spiral shell worm ethyl ester, Buprofezin.
This agricultural insecticidal composite can be configured to multiple dosage form such as suspending agent, aqueous emulsion or microemulsion.To this area
For those of ordinary skill, when preparing dosage form, in addition it is also necessary to creatively select multiple in addition to containing active insecticidal components for the election
Auxiliary agent.When preparing above-mentioned dosage form, can select as needed using different pesticidal preparations auxiliary elements (auxiliary agent).Described auxiliary
Co-ingredients can be one of disperse medium, dispersant, emulsifying agent, wetting agent, thickening agent, defoamer, antifreezing agent, filler etc.
Or it is several.
First the compound method of the corresponding preparations of the agricultural insecticidal composite containing Herba speranskiae tuberculatae extract is illustrated.
When preparing corresponding suspending agent, spendable auxiliary agent has: dispersant selects polycarboxylic acid salt
(tersperse2700, t36 etc.), lignin sulfonate (ufoxane 3a, borresperse na, borresperse
Ca-sa etc.), naphthalene and alkyl naphthalene formaldehyde condensate sulfonates class (nno, mf, morwet d-425, tamol nn,
Tersperse2020 etc.), pull open powder bx (nekal), (polyoxyethylene polyoxypropylene is embedding for eo-po block polyether class
Section copolymer), alkylphenol-polyethenoxy base ether phosphate, in alkylphenol-polyethenoxy base ether formaldehyde condensation products sulfate (sopa)
One or more；Wetting agent selects Sulfateses (k-12), Sulfonateses (abs-na, bx, terwet 1004 etc.), compound profit
One or more in humectant (morwet efw)；Thickening agent select starch, polyvinyl alcohol, sodium carboxymethyl cellulose, one of
Or it is multiple；One in kieselguhr, Kaolin, white carbon, attapulgite, potter's clay, carbamide, corn starch, sodium sulfate selected by filler
Plant or multiple.
When preparing corresponding aqueous emulsion, spendable auxiliary agent has: by (castor oil polyoxyethylene ether) system selected by emulsifying agent
Row emulsifying agent (by-110, by-125, by-140), agriculture breast 400, rather breast 33,32,4203,4103, calcium dodecyl benzene sulfonate,
Fatty alcohol-polyoxyethylene ether, Nongru-700 # (common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201, this disk -60#
(common name: sorbitan monostearate), Tween-60 # (common name: sorbitan monostearate polyoxyethylene ether),
One or more of agriculture breast 1601# (common name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether)；Co-emulsifier such as methanol,
One or more of isopropanol, n-butyl alcohol, ethanol；Ketohexamethylene, n- methyl pyrrolidone, dimethylbenzene, toluene, molten selected by solvent
One or more in agent oil.Stabilizer is selected one or more in triphenyl phosphite, epoxychloropropane；Water is deionized water.
When preparing corresponding microemulsion, spendable auxiliary agent has: calcium dodecyl benzene sulfonate (agriculture breast selected by emulsifying agent
500#), Nongru-700 # (common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201#, this disk -60# (common name: lose
Water sorbitan stearate), Tween-60 # (common name: polyoxyethylene sorbitan stearate), tx-10 (common name: pungent
Base phenol polyethenoxy (10) ether), agriculture breast 1601 (common name: triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer),
One or more in agriculture breast 600#, agriculture breast 400#；Co-emulsifier such as one of methanol, isopropanol, n-butyl alcohol, ethanol or many
Kind；Solvent is selected in Ketohexamethylene, n- methyl pyrrolidone, dimethylbenzene, toluene, solvent naphtha (trade mark: s-150, s-180, s-200)
One or more；Stabilizer is selected one or more in triphenyl phosphite, epoxychloropropane；Water is deionized water.
The Pesticidal combination of the present invention includes the first active component and the second active component, and the first active component is Herba speranskiae tuberculatae
Extract, the second active component be Tolfenpyrad, Affirm (Merck Co.), imidacloprid, capillary, spiral shell worm ethyl ester, in Buprofezin
One kind.It should be noted that the effective ingredient (or active component) in each example of formulations of subordinate is weight after folding hundred.Mirror
In length, the present invention have chosen Tolfenpyrad, Affirm (Merck Co.), imidacloprid, capillary, spiral shell worm ethyl ester, Buprofezin activity
Composition illustrates.
Example of formulations 1
Weigh 250 grams of Herba speranskiae tuberculatae extracts, 50 grams of Tolfenpyrads, 50 grams of sodium lignin sulfonates, 20 grams of pull open powder bx (two fourths
Base sodium naphthalene sulfonate), 30 grams of k-12 (sodium lauryl sulphate), 20 grams of pva (polyvinyl alcohol, molecular weight 2000), 20 grams of polyethylene
100 grams of ketopyrrolidine, Kaolin, water supplies 1000 grams.The frequent regulation of above-mentioned raw materials takes the method for suspending agent to mix, sand mill
Sand milling to particle diameter is less than 5 μm, produces active constituent content for 30% Herba speranskiae tuberculatae extract Tolfenpyrad suspending agent.
Example of formulations 2
Weigh 150 grams of Herba speranskiae tuberculatae extracts, 10 grams of Affirm (Merck Co.)s, 25 grams of t36 (polycarboxylate), 30 grams of pull open powders
Bx (nekal), 20 grams of k-12 (sodium lauryl sulphate), 100 grams of roc profit soil, water supplies 1000 grams.Above-mentioned raw materials
Often regulation takes the method for suspending agent to mix, sand mill sand milling to particle diameter is less than 5 μm, to produce active constituent content be 16% saturating
GUCAO extract Affirm (Merck Co.) suspending agent.
Example of formulations 3
Weigh 200 grams of Herba speranskiae tuberculatae extracts, 40 grams of imidacloprids, 30 grams of alkylphenol polyoxyethylene phosphate esters, 50 grams of polyoxies
Ethylene polyoxypropylene block copolymers, 40 grams of calcium dodecyl benzene sulfonates, 30 grams of Nongru-700 #, 20 grams of castor oil polyoxyethylenes
Ether, 1 gram of polyvinyl alcohol, 100 grams of bentonite, 20 grams of bht, water supplies 1000 grams.Above-mentioned raw materials are blended, with sand mill sand milling extremely
Particle diameter is less than 5 μm, that is, it is 24% Herba speranskiae tuberculatae extract imidacloprid suspending agent that active constituent content is obtained.
Example of formulations 4
Weigh 250 grams of Herba speranskiae tuberculatae extracts, 50 grams of capillaries, 30 grams of alkylphenol polyoxyethylene phosphate esters, 50 grams of polyoxies
Ethylene polyoxypropylene block copolymers, 40 grams of calcium dodecyl benzene sulfonates, 30 grams of Nongru-700 #, 20 grams of castor oil polyoxyethylenes
Ether, 1 gram of polyvinyl alcohol, 100 grams of bentonite, 20 grams of bht, water supplies 1000 grams.Above-mentioned raw materials are blended, with sand mill sand milling extremely
Particle diameter is less than 5 μm, that is, it is 30% Herba speranskiae tuberculatae extract capillary suspending agent that active constituent content is obtained.
Example of formulations 5
Weigh 300 grams of Herba speranskiae tuberculatae extracts, 50 grams of spiral shell worm ethyl esters, 30 grams of fatty alcohol-polyoxyethylene ether, 30 grams of diffusants
Nno, 60 grams of agriculture breasts 500#, 30 grams of agriculture breasts 2201#, 30 grams of polyvinyl alcohol, 100 grams of bentonite, 10 grams of sanloses,
Water supplies 1000 grams.Above-mentioned raw materials are blended, are less than 5 μm with sand mill sand milling to particle diameter, that is, active constituent content is obtained and is
35% Herba speranskiae tuberculatae extract spiral shell worm ethyl ester suspending agent.
Example of formulations 6
Weigh 100 grams of Herba speranskiae tuberculatae extracts, 100 grams of Buprofezins, 40 grams of tx-10,50 grams of Nongru-700 #, 30 grams of agriculture breasts
500#, 40 grams of agriculture breasts 1601#, 150 grams of Ketohexamethylene, 50 grams of n- methyl pyrrolidones, 50 grams of n-butyl alcohol, completely and mix through dissolving
Uniformly, deionized water adds to 1000 grams, and it is 20% Herba speranskiae tuberculatae extract Buprofezin microemulsion that active constituent content is obtained after stirring
Agent.
Example of formulations 7
Weigh 100 grams of Herba speranskiae tuberculatae extracts, 50 grams of Buprofezins, 40 grams of Nongru-700 #, 30 grams of agriculture breasts 500#, 60 grams of agriculture breasts
600#, 100 grams of cyclohexanones, 50 grams of isopropanols, 10 grams of epoxychloropropane, through the complete simultaneously mix homogeneously of dissolving, deionized water adds to
1000 grams, it is 15% Herba speranskiae tuberculatae extract thiazine ketone aqueous emulsion that active constituent content is obtained after stirring.
(3) Biometric portion
1st, indoor virulence experimental section
Toxicity Determination embodiment 1: wild cabbage diamondback moth Toxicity Determination
In order to prevent and treat the diamondback moth on the brassicaceous vegetable vegetable in agricultural production, inventor with Herba speranskiae tuberculatae extract with
The potentiation research that Tolfenpyrad, Affirm (Merck Co.), capillary are mutually compounded.Method particularly includes:
Subjects are diamondback moth, plutella xylostella.Active compound is configured to the test medicine needing, test
Method is with reference to " People's Republic of China (PRC) agricultural industry criteria ny/t1154.6 2006 farm-chemical indoor determination test rule is killed
Worm agent the 6th part: biocidal activity test dip method ", " People's Republic of China (PRC) agricultural industry criteria ny/t1154.7 2006
Farm-chemical indoor determination test rule insecticide the 7th part: the synergy of mixture measures ".
(mortality rate is pressed in the range of 5%～90% first single dose and each Mixed Pharmacy to be arranged 5 variable concentrations gradients
Geometric progressions set).With the pure water added with a small amount of surfactant (Tween 80), choose Individual Size basically identical, healthy
Diamondback moth, standby.Will be equipped with diamondback moth leaching worm cage leaching into the liquid be shaken gently for, suck through taking-up absorbent paper in 5 seconds
Adhesive surface and the medicinal liquid of surrounding, often process 4 repetitions, often repeat 10, and the corresponding organic solvent setting without medicament is processed
As comparison.After process, 48h checks test worm death condition, records total borer population and dead borer population.Dead criterion is: uses little brush pen
Flip the completely motionless person of polypide and be designated as dead worm.
Then move to thermostatic chamber, cultivate under conditions of 20 DEG C～24 DEG C of temperature, process investigation in latter 48 hours and live borer population, meter
Calculate Revision insect recluced rate.
Computational methods:
Revision insect recluced rate=(number of living in after before processing insect population number-process)/before processing insect population number × 100
Prevention effect (%)=[(processing Revision insect recluced rate-comparison Revision insect recluced rate)/(100- compares Revision insect recluced rate)]
×100
By the linear regression analyses between the probit value of preventive effect and the logarithm value of series concentration, obtain the lc of each medicament50
Value, calculates the co-toxicity coefficient (ctc) of mixture, to evaluate the activity to pathogenic bacteria for the reagent agent with this with the abundant method of Sun Yun.
The co-toxicity coefficient (ctc) >=120 of complex preparation shows as potentiation；Ctc≤80 show as antagonism；80 ＜
Ctc ＜ 120 shows as summation action.
The experimental result of the present embodiment is shown in Table 1-1 to table 1-3.
Table 1-1 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to wild cabbage diamondback moth with Tolfenpyrad
| Chemicals treatment | Virulence regression equation | Correlation coefficient (r) | lc50(mg/l) | Co-toxicity coefficient (ctc) |
| Herba speranskiae tuberculatae extract (a) | Y=-0.7019+1.7291x | 0.9764 | 305.93 | - |
| Tolfenpyrad (b) | Y=2.8994+1.8122x | 0.9884 | 14.43 | - |
| A:b=1:5 | Y=2.5372+2.3425x | 0.9904 | 11.26 | 152.35 |
| A:b=1:2.5 | Y=2.9400+1.7778x | 0.9944 | 14.41 | 137.60 |
| A:b=1:1 | Y=3.1320+1.4657x | 0.9881 | 18.82 | 146.44 |
| A:b=2.5:1 | Y=1.8666+2.0285x | 0.9668 | 35.05 | 128.89 |
| A:b=5:1 | Y=-1.0211+2.2927x | 0.9894 | 54.39 | 128.81 |
| A:b=10:1 | Y=1.4754+1.8513x | 0.9886 | 80.15 | 134.57 |
| A:b=15:1 | Y=1.2909+1.8513x | 0.9896 | 100.82 | 134.11 |
Table 1-2 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to wild cabbage diamondback moth with Affirm (Merck Co.)
Table 1-3 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to wild cabbage diamondback moth with capillary
| Chemicals treatment | Virulence regression equation | Correlation coefficient (r) | lc50(mg/l) | Co-toxicity coefficient (ctc) |
| Herba speranskiae tuberculatae extract (a) | Y=-0.7019+1.7291x | 0.9764 | 305.93 | - |
| Capillary (b) | Y=4.650+1.7991x | 0.9850 | 1.55 | - |
| A:b=1:5 | Y=4.6362+2.0231x | 0.9978 | 1.51 | 123.05 |
| A:b=1:2.5 | Y=4.4644+2.2963x | 0.9930 | 1.71 | 126.64 |
| A:b=1:1 | Y=4.2321+2.0995x | 0.9916 | 2.32 | 132.95 |
| A:b=2.5:1 | Y=4.0419+1.5893x | 0.9814 | 4.01 | 133.59 |
| A:b=5:1 | Y=3.4834+1.915x | 0.9753 | 6.19 | 146.53 |
| A:b=10:1 | Y=3.0227+2.0051x | 0.9917 | 9.69 | 167.47 |
| A:b=15:1 | Y=2.1778+2.2465x | 0.9842 | 18.04 | 127.76 |
The Toxicity Determination result of table 1-1 to table 1-3 shows: Herba speranskiae tuberculatae extract and Tolfenpyrad, methylamino AVM hereinafter
One of rhzomorph, capillary, with weight than as 15:1～1:5 use with have higher poison to brassicaceous vegetable wild cabbage diamondback moth
Power, all has significant potentiation.
Toxicity Determination embodiment 2: cabbage pieris Toxicity Determination
In order to prevent and treat the Pieris rapae on the brassicaceous vegetable vegetable in agricultural production, inventor with Herba speranskiae tuberculatae extract with
The potentiation research that Tolfenpyrad, Affirm (Merck Co.), capillary are mutually compounded.Method particularly includes:
Subjects are Pieris rapae.Active compound is configured to the test medicine needing, test method is with reference to " Chinese people's republicanism
State agricultural industry criteria ny/t1154.6 2006 farm-chemical indoor determination test rule insecticide the 6th part: insecticide is lived
Property test dip method ", " People's Republic of China (PRC) agricultural industry criteria ny/t1154.7 2006 farm-chemical indoor determination test
Criterion insecticide the 7th part: the synergy of mixture measures ".
(mortality rate is pressed in the range of 5%～90% first single dose and each Mixed Pharmacy to be arranged 5 variable concentrations gradients
Geometric progressions set).With the pure water added with a small amount of surfactant (Tween 80), choose Individual Size basically identical, healthy
Pieris rapae, standby.Will be equipped with Pieris rapae leaching worm cage leaching into the liquid be shaken gently for, suck through taking-up absorbent paper in 5 seconds
Adhesive surface and the medicinal liquid of surrounding, often process 4 repetitions, often repeat 10, and the corresponding organic solvent setting without medicament is processed
As comparison.After process, 48h checks test worm death condition, records total borer population and dead borer population.Dead criterion is: uses little brush pen
Flip the completely motionless person of polypide and be designated as dead worm.
Then move to thermostatic chamber, cultivate under conditions of 20 DEG C～24 DEG C of temperature, process investigation in latter 48 hours and live borer population, meter
Calculate Revision insect recluced rate.
Computational methods:
Revision insect recluced rate=(number of living in after before processing insect population number-process)/before processing insect population number × 100
Prevention effect (%)=[(processing Revision insect recluced rate-comparison Revision insect recluced rate)/(100- compares Revision insect recluced rate)]
×100
By the linear regression analyses between the probit value of preventive effect and the logarithm value of series concentration, obtain the lc of each medicament50
Value, calculates the co-toxicity coefficient (ctc) of mixture, to evaluate the activity to pathogenic bacteria for the reagent agent with this with the abundant method of Sun Yun.
The co-toxicity coefficient (ctc) >=120 of complex preparation shows as potentiation；Ctc≤80 show as antagonism；80 ＜
Ctc ＜ 120 shows as summation action.
The experimental result of the present embodiment is shown in Table 2-1 to table 2-3.
Table 2-1 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to cabbage pieris with Tolfenpyrad
| Chemicals treatment | Virulence regression equation | Correlation coefficient (r) | lc50(mg/l) | Co-toxicity coefficient (ctc) |
| Herba speranskiae tuberculatae extract (a) | Y=0.0087+2.2093x | 0.9764 | 181.65 | - |
| Tolfenpyrad (b) | Y=3.2355+1.8147x | 0.9835 | 9.38 | - |
| A:b=1:5 | Y=3.1564+1.9253x | 0.9933 | 9.03 | 123.38 |
| A:b=1:2.5 | Y=3.0848+1.9135x | 0.9848 | 10.02 | 128.41 |
| A:b=1:1 | Y=2.5003+2.1584x | 0.9888 | 14.39 | 123.97 |
| A:b=2.5:1 | Y=2.1039+2.2544x | 0.9978 | 19.26 | 150.97 |
| A:b=5:1 | Y=1.7882+2.2084x | 0.9970 | 28.47 | 157.12 |
| A:b=10:1 | Y=1.2067+2.2545x | 0.9978 | 48.15 | 141.32 |
| A:b=15:1 | Y=1.2936+2.0437x | 0.9907 | 65.10 | 129.91 |
Table 2-2 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to cabbage pieris with Affirm (Merck Co.)
Table 2-3 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to cabbage pieris with capillary
| Chemicals treatment | Virulence regression equation | Correlation coefficient (r) | lc50(mg/l) | Co-toxicity coefficient (ctc) |
| Herba speranskiae tuberculatae extract (a) | Y=0.0087+2.2093x | 0.9764 | 181.65 | - |
| Capillary (b) | Y=4.8278+1.8051x | 0.9844 | 1.24 | - |
| A:b=1:5 | Y=4.8352+2.2886x | 0.9950 | 1.18 | 125.93 |
| A:b=1:2.5 | Y=4.7495+2.0158x | 0.9905 | 1.33 | 130.17 |
| A:b=1:1 | Y=4.5202+1.776x | 0.9778 | 1.86 | 132.43 |
| A:b=2.5:1 | Y=3.6725+2.9836x | 0.9935 | 2.79 | 152.95 |
| A:b=5:1 | Y=3.3101+2.4684x | 0.9855 | 4.84 | 148.65 |
| A:b=10:1 | Y=2.8012+2.4684x | 0.9854 | 7.78 | 164.12 |
| A:b=15:1 | Y=2.4213+2.398x | 0.9887 | 11.89 | 151.36 |
The Toxicity Determination result of table 2-1 to table 2-3 shows: Herba speranskiae tuberculatae extract and Tolfenpyrad, methylamino AVM hereinafter
One of rhzomorph, capillary, with weight than as 1:15～5:1 uses with has higher virulence to cabbage pieris, all has notable
Potentiation.
Toxicity Determination embodiment 3: Fructus Mali pumilae scale insect Toxicity Determination
In order to prevent and treat the Fructus Mali pumilae scale insect in agricultural production, inventor is with Herba speranskiae tuberculatae extract and spiral shell worm ethyl ester, Buprofezin
The potentiation research mutually being compounded.Test method is with reference to " People's Republic of China (PRC) agricultural industry criteria ny/t1154.12
2008 farm-chemical indoor determination test rule insecticide the 12nd part: biocidal activity test tetranychid slide infusion process ", " in
Magnificent people's republic agricultural industry criteria ny/t1154.7 2006 farm-chemical indoor determination test rule insecticide the 7th
Point: the synergy of mixture measures ".
Method particularly includes: subjects are Fructus Mali pumilae scale insect, predominantly Fructus Mali pumilae ball a red-spotted lizard (rhodococcus sariuoni),
Incubates nymph, gathers field, does not apply any pesticide before adopting, and polypide growth is consistent.First active compound is configured to the investigational agent needing
Agent, then adopts the leaching slide method that FAO (Food and Agriculture Organization of the United Nation) is recommended, and the segment that two-sided tape is cut into 3cm length is attached to microscope slide one
End, then tears the scraps of paper on viscose glue off with tweezers, chooses basically identical, the healthy scale insect of Individual Size, is provoked with little brush pen,
Its back is attached on viscose glue and make its feeler and foot not to be stuck, every slide glues 30.First transfer 4h in moisturizing condition, with double
Spectroscopy dissected by cylinder, and worm of checkmating is chosen and discards, then mends viscous.Then it is shaken gently for into the liquid for the slide leaching being stained with scale insect,
Suck the medicinal liquid of adhesive surface and surrounding through taking-up absorbent paper in 5 seconds, often process 4 repetitions, often repeat 30, and set and do not contain
The corresponding organic solvent of medicament is processed as comparison.After process, 48h checks test worm death condition, records total borer population and dead borer population,
Calculate Revision insect recluced rate.Dead criterion is: flips the completely motionless person of polypide with little brush pen or the slight springing person of foot is designated as extremely
Worm.
Computational methods:
Revision insect recluced rate=(number of living in after before processing insect population number-process)/before processing insect population number × 100
Prevention effect (%)=[(processing Revision insect recluced rate-comparison Revision insect recluced rate)/(100- compares Revision insect recluced rate)]
×100
By the linear regression analyses between the probit value of preventive effect and the logarithm value of series concentration, obtain the lc of each medicament50
Value, calculates the co-toxicity coefficient (ctc) of mixture, to evaluate the activity to pathogenic bacteria for the reagent agent with this with the abundant method of Sun Yun.
The co-toxicity coefficient (ctc) >=120 of complex preparation shows as potentiation；Ctc≤80 show as antagonism；80 ＜
Ctc ＜ 120 shows as summation action.
The experimental result of the present embodiment is shown in Table 3-1 to table 3-2.
Table 3-1 Herba speranskiae tuberculatae extract and the Toxicity Determination to Fructus Mali pumilae scale insect for the buprofezin compound
| Chemicals treatment | Virulence regression equation | Correlation coefficient (r) | lc50(mg/l) | Co-toxicity coefficient (ctc) |
| Herba speranskiae tuberculatae extract (a) | Y=0.9570+1.6756x | 0.9841 | 258.73 | - |
| Buprofezin (b) | Y=3.0518+1.9679x | 0.9850 | 9.77 | - |
| A:b=1:5 | Y=2.5735+2.4744x | 0.9973 | 9.56 | 121.72 |
| A:b=1:2.5 | Y=2.5075+2.4317x | 0.9967 | 10.59 | 127.24 |
| A:b=1:1 | Y=2.6894+2.0896x | 0.9872 | 12.76 | 147.56 |
| A:b=2.5:1 | Y=1.3835+2.5895x | 0.9899 | 24.92 | 125.38 |
| A:b=5:1 | Y=1.0972+2.5202x | 0.9907 | 35.37 | 139.41 |
| A:b=10:1 | Y=1.5744+1.9659x | 0.9851 | 55.27 | 141.15 |
| A:b=15:1 | Y=1.0123+2.1474x | 0.9905 | 71.94 | 138.72 |
Table 3-2 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to Fructus Mali pumilae scale insect with spiral shell worm ethyl ester
Toxicity Determination result (table 3-1, table 3-2) shows: in Herba speranskiae tuberculatae extract and spiral shell worm ethyl ester, Buprofezin
Kind, with weight than as 15:1～1:5 use with have preferable virulence to Fructus Mali pumilae scale insect, all have significant potentiation.
Toxicity Determination embodiment 4: apple yellow aphid Toxicity Determination
Toxicity Determination embodiment 5: Cucumber blight Toxicity Determination
In order to prevent and treat the aphid of the harm Fructus Mali pumilae in agricultural production, Fructus Cucumidis sativi, inventor is with Herba speranskiae tuberculatae extract and imidacloprid
The potentiation research mutually being compounded.Test method is with reference to " People's Republic of China (PRC) agricultural industry criteria ny/t1154.12
2008 farm-chemical indoor determination test rule insecticide the 12nd part: biocidal activity test tetranychid slide infusion process ", " in
Magnificent people's republic agricultural industry criteria ny/t1154.7 2006 farm-chemical indoor determination test rule insecticide the 7th
Point: the synergy of mixture measures ".Method particularly includes:
Subjects are apple yellow aphid (aphis ciricola), Cucumber blight (aphis gossypii), pick up from field,
Any pesticide is not applied, polypide growth is consistent before adopting.First active compound is configured to the test medicine needing, then adopts the United Nations's grain
The leaching slide method of agriculture Organisation recommendations, the segment that two-sided tape is cut into 3cm length is attached to microscope slide one end, is then torn off viscous with tweezers
The scraps of paper on glue, choose basically identical, the healthy aphid of Individual Size, are provoked with little brush pen, its back is attached on viscose glue and
Its feeler and foot is made not to be stuck, every slide glues 30.First transfer 4h in moisturizing condition, dissect spectroscopy with the bitubular, worm of checkmating
Choose and discard, then mend viscous.Then it is shaken gently for into the liquid for the slide leaching being stained with aphid, inhale through taking-up absorbent paper in 5 seconds
Remove the medicinal liquid of adhesive surface and surrounding, often process 4 repetitions, often repeat 30, and set at without the corresponding organic solvent of medicament
Reason is as comparison.After process, 48h checks test worm death condition, records total borer population and dead borer population, calculates Revision insect recluced rate.Death is sentenced
Disconnected standard is: flips the completely motionless person of polypide with little brush pen or the slight springing person of foot is designated as dead worm.
Computational methods:
Revision insect recluced rate=(number of living in after before processing insect population number-process)/before processing insect population number × 100
Prevention effect (%)=[(processing Revision insect recluced rate-comparison Revision insect recluced rate)/(100- compares Revision insect recluced rate)]
×100
By the linear regression analyses between the probit value of preventive effect and the logarithm value of series concentration, obtain the lc of each medicament50
Value, calculates the co-toxicity coefficient (ctc) of mixture, to evaluate the activity to pathogenic bacteria for the reagent agent with this with the abundant method of Sun Yun.
The co-toxicity coefficient (ctc) >=120 of complex preparation shows as potentiation；Ctc≤80 show as antagonism；80 ＜
Ctc ＜ 120 shows as summation action.
The experimental result of the present embodiment is shown in Table 4-1 to table 4-2.
Table 4-1 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to apple yellow aphid with imidacloprid
| Chemicals treatment | Virulence regression equation | Correlation coefficient (r) | lc50(mg/l) | Co-toxicity coefficient (ctc) |
| Herba speranskiae tuberculatae extract (a) | Y=0.5819+1.8088x | 0.9886 | 277.10 | - |
| Imidacloprid (b) | Y=4.0589+1.8051x | 0.9844 | 3.32 | - |
| A:b=1:5 | Y=3.8221+2.4342x | 0.9941 | 3.05 | 130.31 |
| A:b=1:2.5 | Y=3.8831+2.0876x | 0.9821 | 3.43 | 134.86 |
| A:b=1:1 | Y=3.8098+1.8599x | 0.9721 | 4.36 | 150.49 |
| A:b=2.5:1 | Y=3.5382+1.7149x | 0.9942 | 7.12 | 158.46 |
| A:b=5:1 | Y=2.3860+2.417x | 0.9701 | 12.06 | 155.84 |
| A:b=10:1 | Y=2.2397+2.0879x | 0.9806 | 20.99 | 155.37 |
| A:b=15:1 | Y=2.6202+1.5947x | 0.9781 | 31.07 | 144.92 |
Table 4-2 Herba speranskiae tuberculatae extract compounds the Toxicity Determination to Cucumber blight with imidacloprid
| Chemicals treatment | Virulence regression equation | Correlation coefficient (r) | lc50(mg/l) | Co-toxicity coefficient (ctc) |
| Herba speranskiae tuberculatae extract (a) | Y=0.1380+2.0051x | 0.9789 | 265.96 | - |
| Imidacloprid (b) | Y=5.7994+1.5128x | 0.9898 | 0.30 | - |
| A:b=1:5 | Y=6.3688+2.4949x | 0.9892 | 0.28 | 128.54 |
| A:b=1:2.5 | Y=6.1532+2.3566x | 0.9888 | 0.32 | 131.19 |
| A:b=1:1 | Y=5.7687+1.9951x | 0.9894 | 0.41 | 146.18 |
| A:b=2.5:1 | Y=5.2869+1.7438x | 0.9873 | 0.69 | 151.75 |
| A:b=5:1 | Y=4.8663+2.2394x | 0.9924 | 1.15 | 155.64 |
| A:b=10:1 | Y=4.4462+1.6307x | 0.9742 | 2.19 | 149.00 |
| A:b=15:1 | Y=3.9805+2.0079x | 0.9891 | 3.22 | 146.59 |
Toxicity Determination result (table 4-1 to table 4-2) shows: Herba speranskiae tuberculatae extract and imidacloprid are with weight ratio as 15:1
～1:5 uses with all has higher virulence to apple yellow aphid, Cucumber blight, all has significant potentiation.
2nd, field test part
Field test embodiment 1: preventing and treating brassicaceous vegetable diamondback moth field test
Carry out example of formulations 1 (30% Herba speranskiae tuberculatae extract Tolfenpyrad in Shaanxi Province's Yangling Area within 2015
Suspending agent), example of formulations 2 (16% Herba speranskiae tuberculatae extract Affirm (Merck Co.) suspending agent) and example of formulations 4 (30%
Herba speranskiae tuberculatae extract capillary suspending agent) preventing and treating brassicaceous vegetable diamondback moth field test.
Test method is with reference to " pesticide field efficacy medicine test criterion (one) insecticide control brassicaceous vegetable lepidopterous larvae
Sick gb/t 17980.13-2000 ".Field control effectiveness test is in 5 days-September of September in 2015 13 days in Shaanxi Province's Yangling District northwest agriculture
Woods University of Science and Technology is experimental field carried out, and soil is an animal-drawn seed plough soil, and experimental plot area is 900 square metres.Trial crops are brassicaceous vegetable
(Caulis et Folium Brassicae capitatae), sweet No. 8 in being, controlling object is diamondback moth (plutella xylostella) to kind.Experimental field soil ph value 7.1,
Fertility is preferable, experimental field cultivation condition uniformity.Test medicine and extension rate are shown in Table 5.Separately set blank, often process 4
Secondary repetition, every plot area 30, totally 32 cells, random district's groups arrangement.Using conventional nebulization, mu dispenser liquid 45kg.The
Carry out before being once administered to diamondback moth larvae three age, on the occasion of trophophase, dispenser is once for Caulis et Folium Brassicae capitatae.
Investigation and statistical method:
Insect population is investigated, after medicine, 1d, 3d, 7d investigate population number after dispenser before medicine.During investigation, every cell exists
The fixed 10 plants of Caulis et Folium Brassicae capitataes in centre position, investigate every time on being determined 10 plants of Caulis et Folium Brassicae capitataes.Before dispenser and after dispenser, investigation respectively records 3 every time
Larva below age and Larva.
Drug effect computational methods
Formula in field efficacy basis " pesticide field efficacy medicine test criterion " calculates Revision insect recluced rate and preventing and treating effect
Really.
Security survey: 10 days after the 1st day and medicine after spraying every time, observe medicament and respectively process the impact to Caulis et Folium Brassicae capitatae.
Brassicaceous vegetable (Caulis et Folium Brassicae capitatae) diamondback moth field test results prevented and treated by table 5
Note: in the difference of 0.05 level between lowercase letter process.
Field test results (table 5) show: 30% 120,135 grams of Herba speranskiae tuberculatae extract Tolfenpyrad suspending agent effectively becomes
Point/hectare, 16% 48,54 grams of Herba speranskiae tuberculatae extract Affirm (Merck Co.) suspending agent effective ingredient/hectare, 30% Herba speranskiae tuberculatae
67.5,72 grams of effective ingredient/hectares of extract capillary suspending agent are poor to 1 day preventive effect after wild cabbage diamondback moth dispenser, dispenser
All there is preferable preventive effect within 3 days afterwards, 7 days, 1,3 days after dispenser, 30% 135 grams of Herba speranskiae tuberculatae extract Tolfenpyrad suspending agent is effectively
Composition/hectare, 30% 54 grams of effective ingredient/hectares of Herba speranskiae tuberculatae extract Affirm (Merck Co.) suspending agent, 30% Herba speranskiae tuberculatae
The preventive effect of extract 72 grams of effective ingredient/hectares of capillary suspending agent is better than deep to comparison medicament 10% in 0.05 level
360 grams of effective ingredient/hectares of herb microemulsion.And from the point of view of using dosage, Tolfenpyrad, methylamino Avermectin in three kinds of mixtures
Element and capillary consumption reduce more than 50%, alleviate ambient pressure.
Observe during test, each chemicals treatment does not produce poisoning phenomenon to crop.
After result of the test shows Herba speranskiae tuberculatae extract and Tolfenpyrad, Affirm (Merck Co.) and capillary mixture hence it is evident that
Improve the prevention effect to brassicaceous vegetable diamondback moth, reduce dosage, safety is grown to Caulis et Folium Brassicae capitatae, be preventing and treating diamondback moth
Favourable agents.
Field test embodiment 2: preventing and treating preventing and treating brassicaceous vegetable Pieris rapae field test
Carry out example of formulations 1 (30% Herba speranskiae tuberculatae extract Tolfenpyrad in Shaanxi Province's Yangling Area within 2015
Suspending agent), example of formulations 2 (16% Herba speranskiae tuberculatae extract Affirm (Merck Co.) suspending agent) and example of formulations 4 (30%
Herba speranskiae tuberculatae extract capillary suspending agent) preventing and treating brassicaceous vegetable Pieris rapae field test.
Test method is with reference to " pesticide field efficacy medicine test criterion (one) insecticide control brassicaceous vegetable lepidopterous larvae
Sick gb/t 17980.13-2000 ".Testing ground area is 900 square metres.Trial crops are brassicaceous vegetable (Caulis et Folium Brassicae capitatae), product
Sweet No. 8 in kind being, controlling object is Pieris rapae (pieris rapae).Experimental field soil ph value 7.1, fertility is preferable, experimental field
Cultivation condition uniformity.Test medicine and extension rate are shown in Table 6.Separately set blank, often process 4 repetitions, every cell face
Long-pending 30, totally 32 cells, random district's groups arrangement.Using conventional nebulization, mu dispenser liquid 45kg.It is administered to Pieris rapae for the first time
Carried out before larva three age, on the occasion of trophophase, dispenser is once for Caulis et Folium Brassicae capitatae.
Investigation and statistical method:
Insect population is investigated, after medicine, 1d, 3d, 7d investigate population number after dispenser before medicine.During investigation, every cell exists
The fixed 10 plants of Caulis et Folium Brassicae capitataes in centre position, investigate every time on being determined 10 plants of Caulis et Folium Brassicae capitataes.Before dispenser and after dispenser, investigation respectively records 3 every time
Larva below age and Larva.
Drug effect computational methods
Formula in field efficacy basis " pesticide field efficacy medicine test criterion " calculates Revision insect recluced rate and preventing and treating effect
Really.
Security survey: 10 days after the 1st day and medicine after spraying every time, observe medicament and respectively process the impact to Caulis et Folium Brassicae capitatae.
Brassicaceous vegetable (Caulis et Folium Brassicae capitatae) Pieris rapae field test results prevented and treated by table 6
Note: in the difference of 0.05 level between lowercase letter process.
Field test results (table 6) show: 30% 120,135 grams of Herba speranskiae tuberculatae extract Tolfenpyrad suspending agent effectively becomes
Point/hectare, 16% 48,54 grams of Herba speranskiae tuberculatae extract Affirm (Merck Co.) suspending agent effective ingredient/hectare, 30% Herba speranskiae tuberculatae
67.5,72 grams of effective ingredient/hectares of extract capillary suspending agent are poor to 1 day preventive effect after cabbage pieris dispenser, dispenser
All there is preferable preventive effect within 3 days afterwards, 7 days, 1,3,7 days after dispenser, in the equal no significant difference of 0.05 level between each process.From use
From the point of view of dosage, in three kinds of mixtures, Tolfenpyrad, Affirm (Merck Co.) and capillary consumption reduce more than 50%, alleviate environment
Pressure.
Observe during test, each chemicals treatment does not produce poisoning phenomenon to crop.
After result of the test shows Herba speranskiae tuberculatae extract and Tolfenpyrad, Affirm (Merck Co.) and capillary mixture hence it is evident that
Improve the prevention effect to brassicaceous vegetable Pieris rapae, reduce dosage, safety is grown to Caulis et Folium Brassicae capitatae, be preventing and treating Pieris rapae
Favourable agents.
Field test embodiment 3: preventing and treating apple yellow aphid field test
Carry out example of formulations 3 (24% Herba speranskiae tuberculatae extract in Shaanxi Province Liquan County Shi De town Northern Wei Dynasty village within 2016
Imidacloprid suspending agent) preventing and treating apple yellow aphid field test.
Test method is with reference to " pesticide field efficacy medicine test criterion (one) insecticide control fruit aphid gb/t 17980.9-
2000》.Experimental field soil is an animal-drawn seed plough soil, and experimental plot area is 600 square metres.Trial crops are Fructus Mali pumilae, and kind is Fuji apple.Preventing and treating
Object is yellow aphids (aphis citricola).Experimental field soil ph value 7.1, fertility is preferable, experimental field cultivation condition uniform one
Cause.Test medicine and extension rate are shown in Table 7.Separately set blank, often process 4 times repetition, every 2 plants of fruit trees of cell, totally 20 little
Area, random district's groups arrangement.Using conventional nebulization, mu dispenser liquid 200kg.Phase of containing at the beginning of spraying time apple aphid is carried out, dispenser
Once.
Investigation and statistical method:
Investigate insect population before medicine, investigate population number within 1,3,7,14 days after dispenser after medicine.Investigate before spray
During aphid radix, respectively repeat to fix 2 plants of fruit trees, select 5 brachyplasts at random as listed sampling by all directions five positionses
Fixed point.Investigate every time, all record the aphid amount on continuous 10 leaves of every sampling point.
Drug effect computational methods
Formula in field efficacy basis " pesticide field efficacy medicine test criterion " calculates Revision insect recluced rate and preventing and treating effect
Really.
Security survey: 14 days after the 1st day and medicine after spraying every time, observe medicament and respectively process the impact to apple tree.
Apple aphid field test results prevented and treated by table 7
Note: in the difference of 0.05 level between lowercase letter process.
Field test results (table 7) show: 24% 3000,2000 times of Herba speranskiae tuberculatae extract imidacloprid suspending agent dilution,
Liquid 70% imidacloprid water dispersible granule, 14000 times poor to 1 day preventive effect after apple aphid dispenser, all has within 3,7,14 days after dispenser
Preferably preventive effect, 1,3,7,14 days after dispenser, in the equal no significant difference of 0.05 level between each process.From the point of view of using dosage,
In 24% Herba speranskiae tuberculatae extract imidacloprid suspending agent, imidacloprid consumption reduces 60%, alleviates ambient pressure.
Observe during test, each chemicals treatment does not produce poisoning phenomenon to crop.
Result of the test is imitated hence it is evident that improve the preventing and treating to apple aphid after showing Herba speranskiae tuberculatae extract and imidacloprid mixture
Really, reduce dosage, to apple development safety, be the anti-favourable agents that eliminate aphis.
Field test embodiment 4: preventing and treating Fructus Mali pumilae scale insect field test
(30% Herba speranskiae tuberculatae carries to have carried out example of formulations 5 in Xianyang, Shanxi province city Liquan County Shi De town Northern Wei Dynasty village within 2015
Take thing spiral shell worm ethyl ester suspending agent), example of formulations 6 (20% Herba speranskiae tuberculatae extract Buprofezin microemulsion) preventing and treating Fructus Mali pumilae shell
Worm field test.
Test method is with reference to " pesticide field efficacy medicine test criterion (one) insecticide control Citrus scale insect gb/t
17980.12-2000 ".Trial crops are Fructus Mali pumilae, and kind is Fuji apple, and controlling object is that (principal item is Fructus Mali pumilae ball to scale insect
A red-spotted lizard (rhodococcus sariuoni).3 mu of testing ground area, Apple Culture management is preferably.Test medicine and dosage refer to table
8.Separately set comparison medicament and blank, often process 4 repetitions, every 2 plants of fruit trees of cell, totally 32 cells, random district's groups arrangement.
Using conventional nebulization, mu dispenser liquid 200kg, uniformly spray Fructus Mali pumilae Herb.Dispenser 1 time altogether, in scale insect nymph dispenser.
Investigation and statistical method:
When investigating scale insect radix before spray, respectively repeat to fix 2 plants of fruit trees, select at random by all directions five positionses
Fixed 5 brachyplasts are as the fixed point of listed sampling.Investigation in 3,7,14 days after medicine, investigates every time, all records continuous 10 leaves of every sampling point
On scale insect worm amount.
Preventive effect is calculated according to survey result.14 days after 1st day and medicine after spraying every time, observe medicament and respectively process to Oryza sativa L.
Impact.
Fructus Mali pumilae scale insect field test results prevented and treated by table 8
Note: in the difference of 0.05 level between lowercase letter process.
Field test results (table 8) show: 30% 1500 times, 2000 times of Herba speranskiae tuberculatae extract spiral shell worm ethyl ester suspending agent,
20% 1500 times of Herba speranskiae tuberculatae extract Buprofezin microemulsion, 2000 times and 240 g/l of spiral shell worm ethyl ester suspending agents of comparison medicament
4000 times, 25% 2500 times of wettable powder with buprofezin process to after Fructus Mali pumilae scale insect medicine 3,7,14 days all have preferably anti-
Effect, is substantially better than 10% deep 200 times of preventive effects processing to Fructus Mali pumilae scale insect of herb microemulsion in 0.05 level.
Security survey to Fructus Mali pumilae, observes, each test process are existing to Fructus Mali pumilae no poisoning for 14 days after the 1st day and medicine after spray
As occurring.Test also finds, three kinds of Mixed Pharmacies also have good preventive effect to apple aphid.
Result of the test shows, Herba speranskiae tuberculatae extract respectively with spiral shell worm ethyl ester, Buprofezin mixture after hence it is evident that improve to Fructus Mali pumilae
The prevention effect of scale insect, reduces dosage and drug cost, to apple development safety, is the ideal of preventing and treating Fructus Mali pumilae scale insect
Medicament.
In sum, the compositionss of the present invention are using Herba speranskiae tuberculatae extract and Tolfenpyrad, Affirm (Merck Co.), pyrrole
The two kinds of active components compound scheme of one of worm quinoline, capillary, spiral shell worm ethyl ester, Buprofezin composition, its activity and parasite killing effect
Fruit is not the simple superposition of each composition activity, compared with existing unitary agent, in addition to there is significant insecticidal effect, Er Qieyou
Significantly potentiation, good to crop safety, meet the security requirement of pesticidal preparations.
Claims (7)
1. a kind of agricultural insecticidal composite containing Herba speranskiae tuberculatae extract is it is characterised in that include the first active component Herba speranskiae tuberculatae
Extract, and the second active component Tolfenpyrad, Affirm (Merck Co.), imidacloprid, capillary, spiral shell worm ethyl ester, Buprofezin
One of, the mass ratio of the first active component and the second active component is 15:1～1:5.
2. according to claim 1 the agricultural insecticidal composite containing Herba speranskiae tuberculatae extract it is characterised in that described Herba speranskiae tuberculatae
Extract is prepared by following methods: the Herba speranskiae tuberculatae after being pulverized with organic solvent soaking at room temperature, and supersound extraction filters, sloughs organic
Herba speranskiae tuberculatae extract is obtained final product after solvent.
3. according to claim 2 the agricultural insecticidal composite containing Herba speranskiae tuberculatae extract it is characterised in that described organic molten
Methanol, ethanol, acetone, ethyl acetate, dichloromethane, chloroform or its mixed solvent are selected in agent.
4. according to claim 3 the agricultural insecticidal composite containing Herba speranskiae tuberculatae extract it is characterised in that described Herba speranskiae tuberculatae
Extract is prepared by following methods: after the Herba speranskiae tuberculatae being dried is pulverized, with methanol soaking at room temperature 24h；Adopt supersound extraction 3 times again,
Supersound extraction 30min every time, filters；Repeat to extract 3 times, merging filtrate, concentrating under reduced pressure obtains Herba speranskiae tuberculatae extract.
5. according to claim 1 the agricultural insecticidal composite containing Herba speranskiae tuberculatae extract it is characterised in that described parasite killing group
The dosage form of compound is suspending agent, aqueous emulsion or microemulsion.
6. according to claim 1 the agricultural insecticidal composite containing Herba speranskiae tuberculatae extract preventing and treating crop pests on use
On the way.
7. the agricultural insecticidal composite containing Herba speranskiae tuberculatae extract according to claim 6 is on preventing and treating crop pests
Purposes is it is characterised in that described crop pests include brassicaceous vegetable diamondback moth, Pieris rapae, Fructus Mali pumilae scale insect, apple aphid
Worm, Cucumber blight.
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| CN108353939A (en) * | 2018-02-26 | 2018-08-03 | 渭南市农业技术推广中心 | Agricultural insecticidal composite containing Herba speranskiae tuberculatae extract |
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