CN103262848B

CN103262848B - Insecticidal composition containing cycloxaprid and biological insecticide

Info

Publication number: CN103262848B
Application number: CN201310233352.7A
Authority: CN
Inventors: 郑敬敏; 何爱华; 李明甫; 冯建雄; 张崇斌; 时晓磊; 张�杰; 黄发伟; 韦挺; 侯江利; 孙瑞; 赵海军; 梅红玉; 寇鹏斌; 乌小瑜; 陆海瑞; 刘卫博; 达普妮; 梁百亮
Original assignee: Shaanxi Sunger Road Bio Science Co Ltd
Current assignee: Shaanxi Sunger Road Bio Science Co Ltd
Priority date: 2011-12-04
Filing date: 2011-12-04
Publication date: 2014-06-25
Anticipated expiration: 2031-12-04
Also published as: CN103262848A

Abstract

The invention discloses an insecticidal composition containing cycloxaprid and a biological insecticide, wherein the first active ingredient of the composition is cycloxaprid, the second ingredient of the composition is spinosad or spinetoram, and the weight ratio of the first active ingredient to the second ingredient is 1: 5 to 30: 1. The composition can be prepared into agriculturally acceptable dosage forms of water dispersible granules, wettable powder, suspending agents, missible oil or microemulsion. The insecticidal composition disclosed by the invention is reasonable in components, good in insecticidal effect and low in medication cost; the activity and the insecticidal effect of the insecticidal composition are not the simple superposition of the activity of all components; compared with the existing simple preparations, the insecticidal composition has both a remarkable insecticidal effect and a remarkable synergistic effect, is good in safety and capable of meeting safety requirements of pesticide preparations; and the insecticidal composition disclosed by the invention has a good prevention and treatment effects on the injurious insects of wheat aphids, citrus aphids, cotton aphids, lugus lucorum, rice planthoppers, vegetable thrips and the like.

Description

A kind of Pesticidal combination containing cycloxaprid and biological source insecticide

Technical field

Patent application of the present invention is that invention and created name is a kind of Pesticidal combination containing cycloxaprid and biological source insecticide, and application number is 201110395844.7, and the applying date is the divisional application of 20111204 patent applications.

The present invention relates to a kind of Pesticidal combination, particularly a kind of cycloxaprid is as the first active component, biological source insecticide Avermectin, emamectin-benzoate, multiple killing teichomycin or ethyl pleocidin, as the Pesticidal combination of the second active component, belong to technical field of compounded pesticide.

Background technology

Cycloxaprid is a kind of novel anabasine insecticide, formulated by East China University of Science, chemical name: 9-(6-chloropyridine-3-yl) methyl)-4-nitro-8-oxa--10,11-glyoxalidine also (2,3-a) dicyclo (3,2,1) pungent-3-alkene, English common name: cycloxaprid, chemical molecular formula C ₁₄h ₁₅clN ₄o ₃.Insect is had to interior suction, stomach toxicity and action of contace poison, can be used for preventing and treating Homoptera, coleopteran pest on crops, without cross resistance, also effective to lepidoptera pest with Imidacloprid, Acetamiprid, Diacloden etc.This product using dosage is low, environmentally safe, to beneficial organism without harm or endanger minimum.Aphid to crops such as wheat, cotton, paddy rice, fruit tree, vegetables, plant hopper, aleyrodid, flea beetle, cotton bollworm, diamond-back moth, prodenia litura, armyworm etc. have higher insecticidal activity.

The features such as biological insecticides kind is a lot, and that has found has a kind more than 2000, has the control phase long, efficient, economic, safe, pollution-free, compatible with environment high are that the best agricultural chemicals of current production nuisanceless green agricultural production is selected.Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin are the outstanding persons of this insecticides.These several medicaments have used for many years, exist the risk that resistance increases gradually, the defect that quick-acting is poor.

Avermectin, have another name called acetamiprid, evil the utmost point go out, desinsection fourth, english common name abamectin, chemical molecular formula C ₄₈h ₇₂o ₁₄(B1a) C ₄₇h ₇₀o ₁₄(B1b), it is a kind of macrolide disaccharide compounds, belong to biogenic insecticidal/acaricidal agent, it is the natural products separating from edaphon, insects and mites is had and tags with stomach poison function and have faint fumigation action, and without systemic action, but it has very strong osmosis to blade, can kill subepidermal insect, and the longevity of residure is long.What its mechanism of action was different from general insecticide is that it disturbs neurophysiology activity, stimulate and discharge γ-aminobutyric acid, and γ-aminobutyric acid having inhibitory action to arthropodan nerve conduction, there is paralysis symptom in mite class with insect with medicament after contacting, inertia does not take food, dead after 2-4 days.Because not causing that insect dewaters rapidly, so its lethal effect is slower.In actual application, due to the long-term Avermectin single dose that uses, cause pest resistance to rise year by year, the risk of use cost increase etc.

Emamectin-benzoate, English common name: emamectin benzoate.Chemical name: 4'-table-methylamino-4'-deoxidation abamectin benzoate.Be a kind of efficient, wide spectrum, long insecticidal/acaricidal agent of the longevity of residure, its mechanism of action hinders pest movements nerve information transmission and makes health paralysis dead.The mode of action, take stomach toxicity as main, without interior absorption energy, is used crop epidermal tissue but can effectively infiltrate to crop, thereby is had the longer longevity of residure.Control Lepidoptera, mite class, coleoptera and homoptera pest are had to high activity, easily degraded in soil, noresidue, free from environmental pollution, within the scope of routine dose to useful insect and natural enemy, people, animal safety, can with most of combined use of pesticides.

Multiple killing teichomycin, English common name: spinosad.Multiple killing teichomycin is a kind of macrolides Nuisanceless high-efficient biological source insecticide.The mode of action novelty of pleocidin, can sustained activation target insect acetylcholine nicotine receptor.Can make insect benumb rapidly, paralyse, finally cause death, its desinsection speed can compare favourably with chemical pesticide.Safe, and with current common insecticide without cross resistance.For the biological insecticides of low toxicity, efficient, low-residual, existing efficient insecticidal properties, there is again the characteristic of pair beneficial insect and mammalian safe, the most applicable pollution-free vegetable, Production of fruit application.Multiple killing teichomycin is the insecticide of a kind of low toxicity, efficient, wide spectrum, and insect is had and tagged fast and stomach poison function, and blade is had to stronger osmosis, can kill subepidermal insect, and the longevity of residure is longer, and some insects are had to certain ovicidal action.Without systemic action.Can effectively preventing Lepidoptera, diptera and thrips insect, also can well prevent and treat that in coleoptera and orthoptera, some takes food the pest species of blade in a large number, poor to the control efficiency of sucking pest and mite class.The multiple killing teichomycin of selling is in the market 2.5% suspending agent and 48% suspending agent.

Ethyl pleocidin, English common name: spinetoram, former the effective elements of the medicine is the mixture (ratio is 3:1) of ethyl pleocidin-J and ethyl pleocidin-L, act on the nervous system of insect, the control efficiency that diamond-back moth, beet armyworm, Liriomyza, thrips, prodenia litura, lima bean pod borer have been had.The ethyl pleocidin of selling is in the market 60 grams per liter suspending agents, and it is high that ethyl pleocidin in use exists use cost, the risk that resistance increases, the defect that the lasting period is general.

Inventor is through a large amount of lab screenings and field control effectiveness test, find after cycloxaprid and biological source insecticide Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin mixture, wheat aphid, oranges and tangerines aphid, cotten aphid, rice fulgorid, thrips are had to obvious synergistic effect, can reduce use cost, high to crop safety.

Summary of the invention

The object of the present invention is to provide a kind of component reasonable, significantly synergy, good disinsection effect, the Pesticidal combination of the cycloxaprid that drug cost is low and biogenic substances insecticide.

Another object of the present invention is to the application of the Pesticidal combination that cycloxaprid and biogenic substances insecticide are provided.

In order to overcome the defect of existing unitary agent, technical scheme of the present invention is to solve like this:

Containing the Pesticidal combination of cycloxaprid and biogenic substances insecticide, comprising:

A) the first active component cycloxaprid;

B) the second active component biogenic substances insecticide Avermectin, emamectin-benzoate, multiple killing teichomycin or ethyl pleocidin;

The weight ratio of the first active component and the second active component is 1:5～30:1, is preferably 1:3～15:1, most preferably is 1:1～10:1.

The cumulant (content summation) of the first active component and the second active component is 1%～80% of described composition total weight, be preferably 5%～50%, the size of active component cumulant is also closely related with formulation, as the active component cumulant of microemulsion, is no more than 50%.

The Pesticidal combination of cycloxaprid of the present invention and biogenic substances insecticide is according to method known in those skilled in the art, and the preparation formulation that can prepare is water dispersible granules, wetting powder, suspending agent, missible oil or microemulsion.

For water dispersible granules, those skilled in the art are familiar with the corresponding auxiliary agent of use and complete the present invention very much.Dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate one or more; Wetting agent as in alkyl sulfate, alkylsulfonate, naphthalene sulfonate one or more; Disintegrant as in ammonium sulfate, urea, sucrose, glucose one or more; Binding agent is as one or more in diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family; Filler as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.

To wetting powder, spendable auxiliary agent has: dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate one or more; Wetting agent as in alkyl sulfate, alkylsulfonate, naphthalene sulfonate one or more; Filler as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, precipitated calcium carbonate, imvite, talcum powder, attapulgite, potter's clay one or more.

To suspending agent, spendable auxiliary agent has: dispersant is produced as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate, the HUNTSMAN of Huntsman Corporation of the TERSPERSE 2020(U.S., alkyl naphthalene sulfonic acid salt) in one or more; Emulsifier is as Nongru-700 #(common name: alkyl phenol formaldehyde resin polyoxyethylene ether), this dish-60#(common name: sorbitan monostearate), Tween-60 #(common name: sorbitan monostearate APEO), agriculture breast 1601#(common name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether), one or more in TERSPERSE 4894; Wetting agent as in alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE 2500 one or more; Thickener as in xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate one or more; Preservative as in formaldehyde, benzoic acid, Sodium Benzoate one or more; Defoamer is as organic silicon defoamer; Antifreezing agent as ethylene glycol, propane diols, glycerine, urea, inorganic salts as in sodium chloride one or more, water is deionized water.

For missible oil, spendable auxiliary agent has: emulsifier is as calcium dodecyl benzene sulfonate (agriculture breast 500#), Nongru-700 #(common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201#, Span-60#(common name: sorbitan monostearate), emulsifier T-60(common name: sorbitan monostearate APEO), TX-10(common name: octyl phenol polyoxy ethene (10) ether), agriculture breast 1601#(common name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether), in agriculture breast 600#, agriculture breast 400# one or more; Solvent is as dimethylbenzene, solvent naphtha (S-150, S-180, S-200), toluene, biodiesel, methylated vegetable oil; Cosolvent: in ethyl acetate, methyl alcohol, dimethyl formamide, cyclohexanone, acetone, MEK one or more.

To microemulsion, spendable auxiliary agent has: emulsifier is as calcium dodecyl benzene sulfonate (agriculture breast 500#), Nongru-700 #(common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201#, this dish-60#(common name: sorbitan monostearate), Tween-60 #(common name: sorbitan monostearate APEO), TX-10(common name: octyl phenol polyoxy ethene (10) ether), agriculture breast 1601(common name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether), in agriculture breast 600#, the newborn 400# of agriculture one or more; Assistant for emulsifying agent as in methyl alcohol, isopropyl alcohol, n-butanol, ethanol one or more; Solvent as cyclohexanone, 1-METHYLPYRROLIDONE, dimethylbenzene, toluene, solvent naphtha (trade mark: S-150, S-180, S-200) one or more; Water is deionized water.

Component of the present invention is reasonable, good disinsection effect, and drug cost is low, and its active and insecticidal effect is not the simple superposition of each composition activity, but has significant synergistic effect, good to crop safety, meets the security requirement of pesticidal preparations.The present invention has higher insecticidal activity to wheat aphid, oranges and tangerines aphid, cotton aphid, mirid pentatomid, rice fulgorid, vegetables thrips.

Embodiment

In order to prevent and treat wheat aphid, oranges and tangerines aphid, the cotton aphid in agricultural production, inventor is with cycloxaprid and biogenic substances insecticide: Avermectin, emamectin-benzoate, multiple killing teichomycin or ethyl pleocidin have carried out mutual composite synergy research.It is tested object that test adopts English grain aphid, and concrete grammar is:

English grain aphid (Sitobion avenae), belongs to Homoptera, Aphidiadae.Test medicine cycloxaprid is prepared by literature method by inventor, the former medicine that Avermectin, Methylaminoemamectin benzoate adopt Hebei Weiyuan Biochemical Co., Ltd. to produce, the former medicine that multiple killing teichomycin, ethyl pleocidin Yuan Yaocai The Dow Agrosciences, LLC. produce.By technology department of Shanggezhilu Biosciences Co., Ltd., Shaanxi, former medicine is mixed with to the test medicine needing, test method is with reference to " the agricultural industry criteria NY/T1154.6-2006 of the People's Republic of China (PRC) ".First single dose and each Mixed Pharmacy are arranged to 5 variable concentrations gradients (on the basis of preliminary experiment result, aphid lethality is pressed arithmetic series and set in 5%～90% scope).Examination worm is the aphid of picking up from field, select the aphid of uniformity in laboratory, put into blade-carrying culture dish, every ware 40 cephalont, 4 culture dishes of every processing, repeat 3 times, spray under 50PSI pressure with potter spray tower, spray a ware at every turn, every ware spraying liquid 3ml, then the culture dish of processing is put into 12H/12H illumination box and cultivate, within 48 hours, check dead borer population, calculate lethality.Blank spray equivalent clear water.By the linear regression analysis between the probit value of lethality and the logarithm value of series concentration, obtain the EC of each medicament ₅₀value, with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, evaluates the activity of reagent agent to examination worm with this.

The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.

Toxicity Determination result shows: cycloxaprid has good virulence as 1:5～30:1 is mixed to wheat aphid take weight ratio with the one that biogenic substances insecticide is selected from Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin, all has significant synergistic effect.

In order to prevent and treat the rice hoppers in agricultural production, inventor is with cycloxaprid and biogenic substances insecticide: Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin have carried out mutual composite synergy research.It is tested object that test adopts brown plant-hopper, and concrete grammar is:

Brown plant-hopper formal name used at school Nilaparvata lugens, Homoptera, Delphacidae.By technology department of Shanggezhilu Biosciences Co., Ltd., Shaanxi, former medicine is mixed with to the test medicine needing, test method is with reference to " the agricultural industry criteria NY/T1154.7-2006 of the People's Republic of China (PRC) ".First single dose and each Mixed Pharmacy are arranged to 5 variable concentrations gradients (on the basis of preliminary experiment result, brown plant-hopper lethality is pressed arithmetic series and set in 5%～90% scope).Examination worm is the brown planthopper ovum that picks up from field, in greenhouse, raised for 3 generations with rice seedlings, select uniformity 3 age nymph, be pressed into the culture dish of putting rice seedling (with cotton ball moisturizing processing) diameter 12cm, every ware connects and anaesthetizes 30 of nymphs, every processing connects 4 culture dishes, under 50PSI pressure, spray with potter spray tower, a ware of each spray, every ware spraying liquid 3ml, after insect revives, puts into 12H/12H illumination box by the culture dish of processing and cultivates, within 72 hours, check dead borer population, calculate lethality.Blank spray equivalent clear water.By the linear regression analysis between the probit value of lethality and the logarithm value of series concentration, obtain the LC of each medicament ₅₀value, with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, evaluates the activity of reagent agent to examination worm with this.

Toxicity Determination result shows: cycloxaprid has good virulence as 1:5～30:1 is mixed to rice fulgorid take weight ratio with the one that biogenic substances insecticide is selected from Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin, all has significant synergistic effect.

In order to prevent and treat all kinds of stinkbugs in agricultural production, inventor is with cycloxaprid and biogenic substances insecticide: Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin have carried out mutual composite synergy research.It is tested object that test adopts cotton green plant bug, and concrete grammar is:

Green plant bug, formal name used at school Apolygus lucorum Meyer-D ü r, belongs to Semiptera Miridae.First single dose and each Mixed Pharmacy are arranged to 5 variable concentrations gradients (on the basis of preliminary experiment result, green plant bug lethality is pressed arithmetic series and set in 5%～90% scope).Examination worm is that cotton field gathers green plant bug adult, on indoor fresh kidney beans, raises, and supplies examination to nymphal development of future generation to 3 ages.Fresh cotton leaf of the same size is flooded after 30 s in liquid, under room temperature condition, naturally dry, then put into plastic casing (diameter 8 cm, high 5 cm) in, active healthy, 3 instar larvaes of the same size of access, 15/box, with being with foraminate lid to build.Separately establish blank, repeat 3 times.Put temperature and be 26～28 ℃, relative moisture and be breeding observing in the illumination box of 70% left and right.Within 48 hours, check the death condition of examination worm.By the linear regression analysis between the probit value of lethality and the logarithm value of series concentration, obtain the LC of each medicament ₅₀value, with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, evaluates the activity of reagent agent to examination worm with this.

Toxicity Determination result shows: cycloxaprid has good virulence as 1:5～30:1 is mixed to green plant bug take weight ratio with the one that biogenic substances insecticide is selected from Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin, all has significant synergistic effect.

Below in conjunction with embodiment, content of the present invention is described further.

FORMULATION EXAMPLE 1

Take 70% cycloxaprid, 10% Avermectin, 3% TERSPERSE 2700(polycarboxylate, Huntsman Corporation of the U.S. produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 1% K-12(lauryl sodium sulfate), 2% ammonium sulfate, 1% carboxymethyl cellulose, diatomite adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 80% cycloxaprid Avermectin water dispersible granules.

FORMULATION EXAMPLE 2

Take 20% cycloxaprid, 5% emamectin-benzoate, 3% TERSPERSE 2700,3% sodium lignin sulfonate, 1% Nekal BX, 1%K-12,1% PVA(molecular weight 2000), 10% ammonium sulfate, kaolin adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 25% cycloxaprid emamectin-benzoate water dispersible granules.

FORMULATION EXAMPLE 3

Take 8% cycloxaprid, 40% multiple killing teichomycin, 3% TERSPERSE 2700,2% dispersing agent NNO, 2% Nekal BX, 2%K-12,10% diatomite, 8% urea, kaolin and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 48% cycloxaprid multiple killing teichomycin water dispersible granules.

FORMULATION EXAMPLE 4

Take 18% cycloxaprid, 6% ethyl pleocidin, 4% TERSPERSE 2700,1% Nekal BX, 1%K-12,5% diatomite, 8% ammonium sulfate, attapulgite and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 24% cycloxaprid ethyl pleocidin water dispersible granules.

FORMULATION EXAMPLE 5

Take 20% cycloxaprid, 2% Avermectin, 6% calcium lignosulfonate, 3% alkylnaphthalene sulfonate formaldehyde condensation products, 1% lauryl sodium sulfate, 25% white carbon, kaolin and fill up the weight portion to 100%.Above-mentioned raw materials is prepared into 22% cycloxaprid Avermectin wetting powder through mixing, ultra micro air-flow crushing, hybrid technique step.

FORMULATION EXAMPLE 6

Take 30% cycloxaprid, 1% emamectin-benzoate, 4% calcium lignosulfonate, 5% alkylnaphthalene sulfonate formaldehyde condensation products, 1.5% lauryl sodium sulfate, 20% white carbon, fine particle calcium carbonate and fill up the weight portion to 100%.Above-mentioned raw materials is prepared into 31% cycloxaprid emamectin-benzoate wetting powder through mixing, ultra micro air-flow crushing, hybrid technique step.

FORMULATION EXAMPLE 7

Take 18% cycloxaprid, 18% multiple killing teichomycin, 4% calcium lignosulfonate, 4% Huntsman Corporation of the TERSPERSE 2020(U.S. produces, alkyl naphthalene sulfonic acid salt), 2% lauryl sodium sulfate, 30% white carbon, kaolin fill up the weight portion to 100%.Above-mentioned raw materials is prepared into 36% cycloxaprid multiple killing teichomycin wetting powder through mixing, ultra micro air-flow crushing, hybrid technique step.

FORMULATION EXAMPLE 8

Take 42.5% cycloxaprid, 7.5% ethyl pleocidin, 6% calcium lignosulfonate, 4% alkylnaphthalene sulfonate formaldehyde condensation products, 1.5% lauryl sodium sulfate, 12% white carbon, fine particle calcium carbonate and fill up the weight portion to 100%.Above-mentioned raw materials is prepared into 50% cycloxaprid ethyl pleocidin wetting powder through mixing, ultra micro air-flow crushing, hybrid technique step.

FORMULATION EXAMPLE 9

Take 18% cycloxaprid, 2% Avermectin, 3% Huntsman Corporation of the TERSPERSE 4894(U.S. produces), 1% Huntsman Corporation of the TERSPERSE 2500(U.S. produces), 0.3% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with the cycloxaprid Avermectin suspending agent that makes 20% after sand mill sand milling.

FORMULATION EXAMPLE 10

Take 20% cycloxaprid, 1% emamectin-benzoate, 4%TERSPERSE 4894,1.5%TERSPERSE 2500,1% Huntsman Corporation of the TERSPERSE 2020(U.S. produces), 0.2% aluminium-magnesium silicate, 0.2% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent, deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 21% cycloxaprid emamectin benzoate suspending agent after sand mill sand milling.

FORMULATION EXAMPLE 11

Take 10% cycloxaprid, 20% multiple killing teichomycin, 3%TERSPERSE 4894,1.5%TERSPERSE 2500,1%TERSPERSE 2020,0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 30% cycloxaprid multiple killing teichomycin suspending agent after sand mill sand milling.

FORMULATION EXAMPLE 12

Take 35% cycloxaprid, 5% ethyl pleocidin, 1%TERSPERSE 2020,3% Nongru-700 #, 3% agriculture breast 1601#, 2%TERSPERSE 2500,0.3% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with the cycloxaprid ethyl pleocidin suspending agent that makes 40% after sand mill sand milling.

FORMULATION EXAMPLE 13

Take 24% cycloxaprid, 3% Avermectin, 4% agriculture breast 500#, 2% agriculture breast 600#, 3% agriculture breast 2201#, 15% dimethyl formamide, solvent naphtha S-180 adds to 100% weight portion.Above-mentioned raw materials, through mixing, makes 27% cycloxaprid abamectin emulsifiable concentrate after stirring and dissolving is complete.

FORMULATION EXAMPLE 14

Take 13% cycloxaprid, 2% emamectin-benzoate, 5% agriculture breast 500#, 2% agriculture breast 400#, 3% Nongru-700 #, 10% dimethyl formamide, dimethylbenzene adds to 100% weight portion.Above-mentioned raw materials, through mixing, makes 15% cycloxaprid emamectin-benzoate missible oil after stirring and dissolving is complete.

FORMULATION EXAMPLE 15

Take 6% cycloxaprid, 18% multiple killing teichomycin, 5% agriculture breast 500#, 3% agriculture breast 400#, 3% agriculture breast 600#, 25% cyclohexanone, dimethylbenzene adds to 100% weight portion.Above-mentioned raw materials, through mixing, makes 24% cycloxaprid multiple killing teichomycin missible oil after stirring and dissolving is complete.

FORMULATION EXAMPLE 16

Take 4% cycloxaprid, 16% ethyl pleocidin, 5% agriculture breast 500#, 3% TX-10,4% agriculture breast 1601#, 10% acetone, dimethylbenzene adds to 100% weight portion.Above-mentioned raw materials, through mixing, makes 20% cycloxaprid ethyl pleocidin missible oil after stirring and dissolving is complete.

FORMULATION EXAMPLE 17

Take 8.5% cycloxaprid, 1.5% Avermectin, 4%TX-10,5% Nongru-700 #, 6% agriculture breast 500#, 4% agriculture breast 1601#, 15% cyclohexanone, 5%N-methyl pyrrolidone, 5% n-butanol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 10% cycloxaprid avermectin micro-emulsion after stirring.

FORMULATION EXAMPLE 18

Take 3% cycloxaprid, 2% emamectin-benzoate, 4% Nongru-700 #, 3% agriculture breast 500#, 6% agriculture breast 600#, 10% dimethylbenzene, 5% cyclohexanone, 5% isopropyl alcohol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 5% cycloxaprid emamectin benzoate microemulsion after stirring.

FORMULATION EXAMPLE 19

Take 16% cycloxaprid, 8% multiple killing teichomycin, 5% Nongru-700 #, 3% agriculture breast 500#, 6% agriculture breast 600#, 10% dimethylbenzene, 13% cyclohexanone, 5% ethanol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 24% cycloxaprid multiple killing teichomycin microemulsion after stirring.

FORMULATION EXAMPLE 20

Take 18% cycloxaprid, 3% ethyl pleocidin, 5% Nongru-700 #, 3% agriculture breast 500#, 6% agriculture breast 600#, 5% dimethylbenzene, 20% cyclohexanone, 5% ethanol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 21% cycloxaprid ethyl pleocidin microemulsion after stirring.

Biological Examples 1: control cotton aphid field trial

Inventor has carried out FORMULATION EXAMPLE 5(22% cycloxaprid Avermectin wetting powder in Chengwu County, Shandong Province in 2011), FORMULATION EXAMPLE 2(25% cycloxaprid emamectin-benzoate water dispersible granules), FORMULATION EXAMPLE 19(24% cycloxaprid multiple killing teichomycin microemulsion), the cycloxaprid ethyl pleocidin suspending agent of FORMULATION EXAMPLE 12(40%) control cotton aphid field trial, verified control efficiency, effective dose and the safety to cotton of these several medicaments to cotton aphid.Test method is with reference to " GB/T 17980.75-2004 pesticide field efficacy medicine test criterion (two) insecticide control cotton aphid ".Trial crops is cotton, and controlling object is cotton aphid.Test is located at Zhang Wuzhuan village, careless villages and small towns, Chengwu County Cotton Fields, and soil is clay, middle fertility, the cultivation condition of each community, water and fertilizer management uniformity.Cotton variety is transgenic cotton against pests Lumianyan 15, mid-April feeding block seedlings raising, mid-May transplant.Dispenser on July 25, water consumption is 60kg/667 ㎡, adopts manual sprayer evenly to spray, require each community cotton leaf positive and negative, limit point, bud even medicine.Test medicine and dosage refer to table 4.Separately establish blank, every processing repeats for 4 times, every community area 25 ㎡, and Gong72Ge community, random district group is arranged.

Investigate and statistical method, 5 samplings of experimental plot inner opposite angle line, fix 4 strains at every, fixing 3 of the upper, middle and lower of every strain leaf, 60 leaves of fixing investigation 20 strain altogether, investigation records aphid number alive.Investigation insect population radix before medicine, after medicine, the remaining aphid number of 1 community of each investigation in 1,3,7 day, investigates 4 times altogether.According to the residual worm amount calculation correction preventive effect of each day after insect population cardinal sum medicine before medicine, and carry out statistical analysis.When each investigation drug effect, observe the impact of each treatment region on cotton leaf, bud and other insect simultaneously.

Control efficiency (%)=[1-(borer population after the front borer population × treatment region medicine of check plot medicine)/(the front borer population of borer population × treatment region medicine after the medicine of check plot)] × 100.

After medicine, the 7th Tian Duige community preventive effect is carried out biostatistics Epidemiological Analysis (in table 4), significance of difference comparative result shows: consistent with the significance of difference in 0.01 level in 0.05 level, between 22% cycloxaprid Avermectin wetting powder 12g/ mu, 25% cycloxaprid emamectin-benzoate water dispersible granules 5g/ mu, 24% cycloxaprid multiple killing teichomycin microemulsion 12g/ mu, 40% cycloxaprid ethyl pleocidin suspending agent 7g/ mu, difference is not remarkable, and between other each processing, difference all reaches utmost point significance level; Between 22% cycloxaprid Avermectin wetting powder 10g/ mu, 25% cycloxaprid emamectin-benzoate water dispersible granules 4g/ mu, 24% cycloxaprid multiple killing teichomycin microemulsion 10g/ mu, 40% cycloxaprid ethyl pleocidin suspending agent 6g/ mu, difference is not remarkable, and between other each processing, difference all reaches utmost point significance level; Between 22% cycloxaprid Avermectin wetting powder 8g/ mu, 25% cycloxaprid emamectin-benzoate water dispersible granules 3g/ mu, 24% cycloxaprid multiple killing teichomycin microemulsion 8g/ mu, 40% cycloxaprid ethyl pleocidin suspending agent 5g/ mu, difference is not remarkable, and between other each processing, difference all reaches utmost point significance level; Between 10% cycloxaprid suspending agent 30g/ mu and other each processing, difference reaches utmost point significance level; Between 2% abamectin emulsifiable concentrate 40g/ mu, 5% emamectin-benzoate water dispersible granules 10g/ mu, difference is not remarkable, and between other each processing, difference all reaches utmost point significance level; Between 10% multiple killing teichomycin suspending agent 25g/ mu, 6% ethyl pleocidin suspending agent 40g/ mu, difference is not remarkable, and between other each processing, difference all reaches utmost point significance level.

Field trial shows: 22% cycloxaprid Avermectin wetting powder, 25% cycloxaprid emamectin-benzoate water dispersible granules, 24% cycloxaprid multiple killing teichomycin microemulsion, 40% cycloxaprid ethyl pleocidin suspending agent has more significant control efficiency to cotton aphid, consumption is respectively 8g/ mu, 10g/ mu, 12g/ mu, 3g/ mu, 4g/ mu, 5g/ mu, 8g/ mu, 10g/ mu, 12g/ mu, 5g/ mu, 6g/ mu, 7g/ mu, to cotton aphid after medicine 1 day preventive effect more than 83.24%, after medicine, 1 day preventive effect is more than 88.66%, after medicine, 1 day preventive effect is at 91.63%-98.88%, obviously be better than contrasting medicament 10% cycloxaprid suspending agent 30g/ mu, 2% abamectin emulsifiable concentrate 40g/ mu, 5% emamectin-benzoate water dispersible granules 10g/ mu, 10% multiple killing teichomycin suspending agent 25g/ mu, the control efficiency of 6% ethyl pleocidin suspending agent 40g/ mu to cotton aphid.

Security survey, observes for after dispenser 1,3,7 day, and all reagent agents, to cotton growth safety, occur without poisoning phenomenon.

Result of the test shows, after cycloxaprid and Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin mixture, obviously to have improved the control efficiency to cotton aphid, has reduced dosage, to cotton growth safety.

Biological Examples 2: prevent and treat rice hoppers field trial

Inventor has carried out the cycloxaprid Avermectin suspending agent of FORMULATION EXAMPLE 9(20% in Zhanjiang, Guangdong Province on May 18th, 2011), FORMULATION EXAMPLE 6(31% cycloxaprid emamectin-benzoate wetting powder), FORMULATION EXAMPLE 7(36% cycloxaprid multiple killing teichomycin wetting powder), FORMULATION EXAMPLE 4(24% cycloxaprid ethyl pleocidin water dispersible granules) prevent and treat the field control effectiveness test of rice hoppers, verified control efficiency, effective dose and the safety to paddy rice of these several medicaments to rice fulgorid.Test is located at citizen An Zhen great Shu village, Zhanjiang, and trial crops is paddy rice, and be the shooting stage breeding time, the cultivation condition of each community, water and fertilizer management uniformity.Controlling object: brown planthopper (Nilaparvata lugens).Test medicine and dosage refer to table 5, separately establish blank.Every processing repeats for 4 times, every community area 25 ㎡, and Gong72Ge community, random district group is arranged.Water consumption is 45kg/667 ㎡, adopts manual sprayer evenly to spray.Respectively within before medicine, after medicine 3 days, 7 days, respectively carrying out once residual borer population investigation.Take the random sampling of balance-jump formula, every community 10 points, 2 clumps every (totally 20 clumps), with basin bat method record rice fulgorid live borer population, by the borer population calculation correction preventive effect before medicine, after medicine.Viewing test medicament has or not poisoning to produce to paddy rice simultaneously.

Computational methods:

Field test results is as follows: 20% cycloxaprid Avermectin suspending agent 8g/ mu, 10g/ mu, 12g/ mu, 31% cycloxaprid emamectin-benzoate wetting powder 6g/ mu, 7g/ mu, 8g/ mu, 36% cycloxaprid multiple killing teichomycin wetting powder 6g/ mu, 7g/ mu, 8g/ mu, 24% cycloxaprid ethyl pleocidin water dispersible granules 8g/ mu, 9g/ mu, 10g/ mu, the preventive effect of brown plant-hopper was respectively to 86.1% in 3 days after dispenser, 88.3%, 92.8%, 87.0%, 88.8%, 92.3%, 87.3%, 89.5%, 93.3%, 86.4%, 90.1%, 94.3%, the preventive effect of the 7th day is respectively 87.9%, 91.7%, 94.6%, 88.9%, 93.0%, 95.4%, 92.1%, 92.9%, 96.4%, 89.5%, 91.3%, 95.2%.Obviously be better than 10% cycloxaprid suspending agent 30g/ mu, 2% abamectin emulsifiable concentrate 40g/ mu, 5% emamectin-benzoate water dispersible granules 10g/ mu, 10% multiple killing teichomycin suspending agent 25g/ mu, 6% ethyl pleocidin suspending agent 40g/ mu to rice fulgorid medicine after the preventive effect of 3 days and 7 days.

Security survey, observes for after dispenser 1,3,7 day, and all reagent agents, to paddy growth safety, occur without poisoning phenomenon.

Test discovery, 20% cycloxaprid Avermectin suspending agent, 31% cycloxaprid emamectin-benzoate wetting powder also show excellent preventive effect to rice leaf roller.

Result of the test shows, after cycloxaprid and Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin mixture, has obviously improved the control efficiency to Brown Planthopper, has reduced dosage, to paddy growth safety.

Biological Examples 3: control joint melon thrips field trial

Inventor has carried out FORMULATION EXAMPLE 17(10% cycloxaprid avermectin micro-emulsion in Baiyun District, Guangzhou City on June 10th, 2011), FORMULATION EXAMPLE 10(21% cycloxaprid emamectin benzoate suspending agent), FORMULATION EXAMPLE 11(30% cycloxaprid multiple killing teichomycin suspending agent), FORMULATION EXAMPLE 20(21% cycloxaprid ethyl pleocidin microemulsion) field control effectiveness test of control joint melon thrips, verified control efficiency, effective dose and the safety to cucumber of these several medicaments to joint melon thrips.Test is located at Taihe county town, Baiyun District, and trial crops is cucumber, and breeding time is for coming into bloom, the cultivation condition of each community, water and fertilizer management uniformity.Controlling object is joint melon thrips.Test medicine and dosage refer to table 6, separately establish blank.Every processing repeats for 4 times, every community area 20 ㎡, and Gong72Ge community, random district group is arranged.With amount of liquid medicine be 60kg/667 ㎡, adopt manual sprayer evenly spray.Respectively within before medicine, after medicine 1 day, 3 days, 7 days, respectively carrying out once residual borer population investigation.The sampling of 5 of every communities, fixes 3 leaves at every in cucumber plant middle and upper part, record thrips borer population alive, and calculate preventive effect.Viewing test medicament has or not poisoning to produce to cucumber simultaneously.

Computational methods:

Field test results shows, 10% 2500 times of cycloxaprid avermectin micro-emulsions, 3000 times, 3500 times, 21% 4500 times of cycloxaprid emamectin benzoate suspending agents, 5000 times, 5500 times, 30% 6000 times of cycloxaprid multiple killing teichomycin suspending agents, 6500 times, 7000 times, 21% 6000 times of cycloxaprid ethyl pleocidin microemulsions, 6500 times, 7000 times have good control efficiency to joint melon thrips, after medicine 1, 3, 7 world preventive effects are between 89.8%-100%, obviously be better than 1500 times of 10% cycloxaprid suspending agents, 2% 1500 times of abamectin emulsifiable concentrates, 1% 1000 times of emamectin-benzoate missible oil, 10% 2000 times of multiple killing teichomycin suspending agents, 6% 1000 times of ethyl pleocidin suspending agents to joint melon thrips medicine after 1, 3, the preventive effect of 7 days.

Security survey, observes for after dispenser 1,3,7 day, and all reagent agents, to cucumber growth safety, occur without poisoning phenomenon.

Test discovery, 10% cycloxaprid avermectin micro-emulsion, 21% cycloxaprid emamectin benzoate suspending agent, 30% cycloxaprid multiple killing teichomycin suspending agent, 21% cycloxaprid ethyl pleocidin microemulsion, 10% cycloxaprid suspending agent also show excellent preventive effect to trialeurodes vaporariorum.

Result of the test shows after cycloxaprid and Avermectin, emamectin-benzoate, multiple killing teichomycin, ethyl pleocidin mixture, has obviously improved the control efficiency to joint melon thrips, has reduced dosage, to cucumber growth safety.

In sum, composition of the present invention is to adopt two kinds of active component compound schemes, its activity and insecticidal effect are not the simple superposition of each composition activity, compared with existing unitary agent, except having significant insecticidal effect, and have significant synergistic effect, and good to crop safety, meet the security requirement of pesticidal preparations.

Claims

1. containing a Pesticidal combination for cycloxaprid and biological source insecticide, it is characterized in that composition active component is:

A) the first active component cycloxaprid;

B) the second active component multiple killing teichomycin or ethyl pleocidin;

The weight ratio of the first active component and the second active component is 1:5～30:1.

2. according to claim 1 containing the Pesticidal combination of cycloxaprid and biological source insecticide, it is characterized in that: the weight ratio of the first active component and the second active component is 1:3～15:1.

3. according to claim 2 containing the Pesticidal combination of cycloxaprid and biological source insecticide, it is characterized in that: the weight ratio of the first active component and the second active component is 1:1～10:1.

4. according to one of any described Pesticidal combination containing cycloxaprid and biological source insecticide of claims 1 to 3, it is characterized in that: the cumulant of the first active component and the second active component is described composition total weight 1%～80%.

5. according to claim 4 containing the Pesticidal combination of cycloxaprid and biological source insecticide, it is characterized in that: the cumulant of the first active component and the second active component is described composition total weight 5%～50%.

6. according to claim 1 containing the Pesticidal combination of cycloxaprid and biological source insecticide, it is characterized in that: described composition is water dispersible granules, wetting powder, suspending agent, missible oil or microemulsion.

7. described in claim 1, contain the Pesticidal combination of cycloxaprid and biological source insecticide in the purposes of preventing and treating in wheat aphid, oranges and tangerines aphid, cotton aphid, mirid pentatomid, rice fulgorid, vegetables thrips insect.