CN103340946A - Method for improving tolerance of parasitic wasp by botanical extract - Google Patents
Method for improving tolerance of parasitic wasp by botanical extract Download PDFInfo
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- CN103340946A CN103340946A CN2013102916785A CN201310291678A CN103340946A CN 103340946 A CN103340946 A CN 103340946A CN 2013102916785 A CN2013102916785 A CN 2013102916785A CN 201310291678 A CN201310291678 A CN 201310291678A CN 103340946 A CN103340946 A CN 103340946A
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Abstract
The invention discloses a method for improving tolerance of parasitic wasp by an agrimonia pilosa ethanol extract. In order to solve the problem that the natural control function of the parasitic wasp on injurious insects is weakened due to the adverse effect of a pesticide on the parasitic wasp in the natural world, meteorus pulchricornis is processed by an ethyl acetate extract of the agrimonia pilosa ethanol extract, and thus, the tolerance of the meteorus pulchricornis on abamectin can be obviously improved. According to the technique, the parasitic wasp is taken as a target object, the drug tolerance of the meteorus pulchricornis is actively improved by the ethyl acetate extract of the agrimonia pilosa ethanol extract, and thus, the pest control effect of the meteorus pulchricornis can be improved. The technique has a significant effect on improvement of the tolerance of the parasitic wasp, is high in safety, and can be used for effectively improving the biological control level of the injurious insects after being is applied to production.
Description
One, technical field
The invention belongs to the biological control of insect pests technical field, be specifically related to a kind of chemical sproof method of Herba Agrimoniae extract raising parasitic wasp of utilizing.
Two, technical background
Current, the emergent prevention and control of agricultural pests mainly rely on chemical control, and many medicaments are when efficiently killing off the insect pests fast, and also the natural enemy to insect has produced adverse effect.Parasitic wasp just often is subjected to the threat of insecticide as the important pest natural enemy of a class.Insecticide is of a great variety, wherein surely belongs to the organic phosphates medicament to what parasitic wasp had the greatest impact.As triazophos etc. snout moth's larva of rice egg parasitoids one rice borer trichogramma is had direct killing effect, it is also higher that chlopyrifos etc. nibble toxicity such as chalcid fly to diamond-back moth braconid wasp and diamond-back moth.Pyrethrin pesticide also has considerable influence to parasitic wasp, as pyrethroid pesticide the white cocoon honeybee of base of a fruit moth moth is had very high toxicity.Avilamycin etc. are antibiotic insecticide efficient, wide spectrum, and it is also relatively poor to the selectivity of target pest and parasitic wasp equally, diamondback moth parasitic wasp diamond-back moth braconid wasp and diamond-back moth are nibbled chalcid fly all have harmful effect.How initiatively to promote parasitic wasp to the toleration of medicament, to give full play to the ability of control evil naturally of parasitic wasp, have important function for improving the nuisanceless control level of insect.Yet it is also rarely found to the research report of the toleration aspect of medicament with regard to how initiatively to promote parasitic wasp by effective measures both at home and abroad.
Herba Agrimoniae Agrimonia pilosa Ledeb. is a kind of Rosaceae herbaceos perennial, in a lot of areas of China distribution is arranged, and it has effects such as antitumor, anti-inflammatory and antalgic, blood sugar lowering, blood pressure lowering and enhancing human body immunity function as a kind of Chinese medicine.The outstanding cocoon honeybee Meteorus pulchricornis of macle is a kind of insecticide endoparasitism honeybee, belong to the capacitive parasitic wasp, its host range is wider, can parasitize the larva of many lepidoptera pests such as beet armyworm, Prodenia litura, bollworm, gypsymoth, is their important parasite.The inventor has found that hairyvein agrimonia herb and bud ethanol extract has the effect that improves the outstanding cocoon honeybee parasitic ability of macle early stage.The present invention studies the extract of hairyvein agrimonia herb and bud ethanol extract first to the influence of the outstanding cocoon honeybee tolerance of macle medicament ability, to excavate effective raising parasitic wasp to the approach of medicament toleration, thereby promoting the biological control of insect pests level for the ability that improves parasitic wasp control insect, result of study has great importance.
Three, summary of the invention
1, goal of the invention
Provide a kind of plant source extract that utilizes to improve the outstanding chemical sproof method of cocoon honeybee of macle, can improve the outstanding cocoon honeybee of macle safely and effectively to the control effect of insect, thereby promote the Biological control level of insect.
2, technical scheme
For achieving the above object, the present invention selects for use the ethanol extraction of Herba Agrimoniae again through the outstanding cocoon honeybee of different organic solvents extraction post processing macle, obtained a kind of chemical sproof method of parasitic wasp that improves safely and efficiently, its application has significantly improved parasitic wasp does the time spent at medicament survival rate.
The present invention is the outstanding cocoon honeybee drug resistance raising method of a kind of macle, it is characterized in that utilizing the extract of hairyvein agrimonia herb and bud ethanol extract to handle the outstanding cocoon honeybee of macle, improves parasitic wasp to the tolerance of medicament.
3, beneficial effect
Method of the present invention compared with prior art, produce following beneficial effect: breaking through traditional Pest control is the target object with the insect, is the target object with the outstanding cocoon honeybee of insect parasite macle, improves the drug resistance of parasitic wasp, with the raising pest control effect, thereby improve the biological control of insect pests level conscientiously.
Four, the specific embodiment
Embodiments of the invention are the methods that adopt indoor measurement.
For trying material:
Herba Agrimoniae is buied by pharmacy of Tongrentang;
The outstanding cocoon honeybee of macle is indoor feeding, raises as the host with beet armyworm;
1.8% avilamycin EC is that Hebei Weiyuan Biochemical Co., Ltd. produces.
Test method:
The ethanol extraction of Herba Agrimoniae and the extraction of different solvents
With Universalpulverizer Herba Agrimoniae is pulverized, taken by weighing 200g dry powder and put into the 1000mL triangular flask, add 95% ethanol 450mL, lixiviating solution is poured out in lixiviate at room temperature 5 days, adds 95% ethanol 400mL again, lixiviate 5 days merges all extracting solution, filters, Rotary Evaporators concentrates, and gets concentrated solution, lyophilization 24h, get crude extract, exsiccator is put in sealing, and is standby.Accurately take by weighing a certain amount of (deciding on the crude extract amount) ethanol extraction, adding distil water 500mL, the ultrasound wave hydrotropy is in the 1000mL separatory funnel of packing into, respectively extract 5 times (300mL/ time) with petroleum ether, chloroform, ethyl acetate successively, merge the extract of each solvent separately, wherein, extract water insoluble and above-mentioned organic solvent is that emulsion layer is separately as a kind of extract, all extracts concentrate through Rotary Evaporators, get concentrated solution, lyophilization 24h gets extract.Three kinds of extracts are all used acetone solution, are stored in 4 ℃ of refrigerators standby.The concentration of Herba Agrimoniae extract is represented as follows: concentration=medical material dry weight/final volume.
Each extract of hairyvein agrimonia herb and bud ethanol extract is to the outstanding chemical sproof influence of cocoon honeybee of macle
Three kinds of extracts of Herba Agrimoniae are all used two kinds of concentration, i.e. 0.4g/mL and 0.2g/mL, and avilamycin is then used 0.9mg/L concentration.Accordingly, take by weighing each solvent extract of a certain amount of Herba Agrimoniae, and measure a certain amount of avilamycin, be made into 10% hydromel that contains 0.4g/mL Herba Agrimoniae acetic acid ethyl ester extract and 0.9mg/L avilamycin respectively, 10% hydromel that contains 0.4g/mL Herba Agrimoniae petroleum ether extract and 0.9mg/L avilamycin, 10% hydromel that contains 0.4g/mL Herba Agrimoniae chloroform extract and 0.9mg/L avilamycin, 10% hydromel that contains 0.2g/mL Herba Agrimoniae acetic acid ethyl ester extract and 0.9mg/L avilamycin, 10% hydromel that contains 0.2g/mL Herba Agrimoniae petroleum ether extract and 0.9mg/L avilamycin, 10% hydromel that contains 0.2g/mL Herba Agrimoniae chloroform extract and 0.9mg/L avilamycin, with its extra-nutrition as parasitic wasp, also prepare 10% hydromel that contains the 0.9mg/L avilamycin simultaneously, and 10% hydromel in contrast.Processing method is as follows: hydromel is added on the filter paper bar, adds 0.2ml on each filter paper bar, the filter paper bar hangs in the disposable cup, puts into 12 parasitic wasps for every glass, every processing 3 times (cup).Handle and add up the parasitic wasp survival condition back every day.
Carry out statistical analysis and the comparison of parasitic wasp survival rate with DPS software.
Implementation result:
Embodiment 1 each extract of 0.4g/mL hairyvein agrimonia herb and bud ethanol extract is to the outstanding chemical sproof influence of cocoon honeybee of macle
The different extracts of 0.4g/mL hairyvein agrimonia herb and bud ethanol extract have been studied to the influence (table 1) of the outstanding cocoon honeybee of macle to the avilamycin toleration.The outstanding cocoon honeybee of macle is tolerated the influence of 0.9mg/L avilamycin from ethyl acetate, chloroform and the petroleum ether extract of 0.4g/ml hairyvein agrimonia herb and bud ethanol extract, after handling 4 days, 0.4g/mL the acetic acid acetic acid extraction thing of hairyvein agrimonia herb and bud ethanol extract has significantly improved the survival rate of parasitic wasp, the survival rate of parasitic wasp is 94% with 0.9mg/L avilamycin Combined Processing the time, and single survival rate with 0.9mg/L avilamycin processed group only has 22%.Medicament individual processing group parasitic wasp is in processing all death in back 6 days, and acetic acid ethyl ester extract and medicament Combined Processing group parasitic wasp survival rate are 50%, significant difference between the two, the acetic acid ethyl ester extract of 0.4g/mL hairyvein agrimonia herb and bud ethanol extract have significantly improved the toleration of the outstanding cocoon honeybee of macle to the 0.9mg/L avilamycin.
Each extract of table 1 0.4g/mL hairyvein agrimonia herb and bud ethanol extract is to the outstanding chemical sproof influence of cocoon honeybee of macle
Embodiment 2 each extract of 0.2g/mL hairyvein agrimonia herb and bud ethanol extract are to the outstanding chemical sproof influence of cocoon honeybee of macle
The different extracts of 0.2g/mL hairyvein agrimonia herb and bud ethanol extract have been studied to the influence (table 2) of the outstanding cocoon honeybee of macle to the avilamycin toleration.The outstanding cocoon honeybee of macle is tolerated the influence of 0.9mg/L avilamycin from ethyl acetate, chloroform and the petroleum ether extract of 0.2g/mL hairyvein agrimonia herb and bud ethanol extract, in back 3 days of processing, the survival rate of each extract processed group parasitic wasp of 0.2g/mL hairyvein agrimonia herb and bud ethanol extract does not all have significant difference with single with the survival rate of 0.9mg/L avilamycin processed group.Medicament individual processing group parasitic wasp is in processing all death in back 4 days, and this moment, acetic acid ethyl ester extract and medicament Combined Processing group parasitic wasp survival rate were 66.7%, significant difference between the two, the survival rate of petroleum ether extract and medicament Combined Processing group and chloroform extract and medicament Combined Processing group parasitic wasp also all reaches significant difference with chemicals treatment group parasitic wasp survival rate.
Each extract of table 2 0.2g/mL hairyvein agrimonia herb and bud ethanol extract is to the influence of the outstanding cocoon honeybee tolerance of macle avilamycin
Claims (2)
1. one kind is utilized the plant source extract to improve the chemical sproof method of insect parasitic wasp, it is characterized in that utilizing the extract of hairyvein agrimonia herb and bud ethanol extract to handle parasitic wasp, has improved the toleration of parasitic wasp to insecticide.
2. the extract of hairyvein agrimonia herb and bud ethanol extract according to claim 1 is characterized in that utilizing the acetic acid ethyl ester extract of hairyvein agrimonia herb and bud ethanol extract to improve the toleration of the outstanding cocoon honeybee of macle to avilamycin to the chemical sproof raising effect of parasitic wasp.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101396446A (en) * | 2008-10-06 | 2009-04-01 | 江苏省农业科学院 | Method for increasing the parasitism performance of parasitic wasp |
| CN102630710A (en) * | 2012-04-10 | 2012-08-15 | 江苏省农业科学院 | Method for increasing infectivity of Paecilomyces fumosoroseus on Bemisia tabaci by utilizing plant source extract |
| CN102715200A (en) * | 2012-06-29 | 2012-10-10 | 陈士忠 | Pollution-free biological pesticide insecticide |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101396446A (en) * | 2008-10-06 | 2009-04-01 | 江苏省农业科学院 | Method for increasing the parasitism performance of parasitic wasp |
| CN102630710A (en) * | 2012-04-10 | 2012-08-15 | 江苏省农业科学院 | Method for increasing infectivity of Paecilomyces fumosoroseus on Bemisia tabaci by utilizing plant source extract |
| CN102715200A (en) * | 2012-06-29 | 2012-10-10 | 陈士忠 | Pollution-free biological pesticide insecticide |
Non-Patent Citations (1)
| Title |
|---|
| 李旭涛等: "中草药在养蜂业中的应用现状及展望", 《中国养蜂》, vol. 54, no. 01, 31 December 2003 (2003-12-31), pages 31 - 32 * |
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