CN102578006B

CN102578006B - Annual culturing method for marine medaka in lab

Info

Publication number: CN102578006B
Application number: CN201110434197.6A
Authority: CN
Inventors: 穆景利; 靳非; 王菊英; 张志锋; 王新红
Original assignee: National Marine Environmental Monitoring Center
Current assignee: National Marine Environmental Monitoring Center
Priority date: 2011-12-22
Filing date: 2011-12-22
Publication date: 2015-02-25
Anticipated expiration: 2031-12-22
Also published as: CN102578006A

Abstract

The invention discloses an annual culturing method for marine medaka in a lab. Culturing conditions include: culture seawater is 28-30 in salinity and 4-8mg/L in dissolved oxygen level after subjected to five-stage filtering via a nylon film, biological adsorbent, activated carbon, biochemical filter and ultraviolet sterilization system; the culture water is at 26-30 DEG C; the culture density reaches 0.2-0.5L per fish in the growth period and reaches 0.5-1L per fish in the breeding period; a light cycle includes 14 hours of illumination and 10 hours of no illumination, and illumination is performed from 5:00 to 19:00; the culture male-to-female ratio is 2:3; in terms of feeding frequency and feeding time, the fishes are fed with marine tropical fish feed three times a day, from 7:30 to 8:30, from 11:30 to 12:30 and from 15:30 to 16:30, and are fed with Artemia larvae twice a day, from 9:45 to 10:15 and from 13:45 to 14:15. By the annual culturing method, the defects that breeding conditions are difficult to control, cost is high, continuous egg laying fails, egg laying amount is low, breeding conditions are limited, and the like are overcome.

Description

The laboratory anniversary cultural method of the blue or green Medaka of seawater

Technical field

The present invention relates to the cultural method of a kind of fish, particularly the laboratory anniversary cultural method of the blue or green Medaka of a kind of seawater (having another name called the blue or green Medaka of stain).

Background technology

Lack generally acknowledged ocean model fingerling in the world in order to carry out marine ecology toxicologic study at present.The blue or green Medaka of stain ( oryzias melastigma) at marine site ubiquities such as China, Korea, Japan and India, its generation cycle is short, genome is less, spawning rate is high, be easy to observation, salinity adaptation scope is wide, has the potentiality becoming ocean model biology.The relevant scholar on the ground such as current China, Japan, Taiwan and Korea S and the mechanism multinomial ecological toxicology Study and appliance that it can be used as new test fingerling to carry out.

The blue or green Medaka body of stain is white, and the flat-sided back of the body is short, and there is a pair larger anal fin at health rear portion.Eye is large in blue.Long 3.5 ~ the 4cm of the individual body of sexual maturity.Male and femalely very easily to distinguish from form.The blue or green Medaka of stain is oviparity, and embryo is hatched after 14 days about fertilization greatly at 28 DEG C.The juvenile fish of new hatching is about ripe through 3 months and lay eggs.The blue or green Medaka of stain is distributed in the top layer in Perenniporia martius marine site, likes a large amount of illumination, and illumination is more strong better, but the illumination continued can upset the rhythm and pace of moving things of the blue or green Medaka breeding of stain.The blue or green Medaka of stain can bear the salinity range from 0 ~ 60ppt, and can survive in the scope of 4 ~ 38 DEG C.26 ~ 30 DEG C is the suitableeest the bread worm, and when temperature gradients, the blue or green Medaka of stain can bear a wide range of temperature fluctuation.

There is following problem in the blue or green Medaka of current Laboratory culture stain: 1. cultivating condition difficulty controls and differs; 2. egg laying amount is few, and incubation rate is low; 3. can not continue to lay eggs; 4. easily there is disease; 5. artificial seawater cost is high.

Therefore the cultural method of the blue or green Medaka of a kind of new stain that can solve the problem is needed now.

Summary of the invention

The present invention is the above-mentioned deficiency in order to solve existing for prior art, a kind of method that can realize stain blue or green Medaka adult fish laboratory large-scale farming is proposed, in order to solve, cultivating condition difficulty controls, cost is high, can not continue to lay eggs, egg laying amount is few and Reproduction Conditions exists the blue or green Medaka of seawater (the stain green grass or young crops Medaka of the aspect defects such as restriction oryzias melastigma) laboratory anniversary cultural method.

Technical solution of the present invention is: the blue or green Medaka of a kind of seawater (the blue or green Medaka of stain, oryzias melastigma) laboratory anniversary cultural method, it is characterized in that: the cultivating condition of described cultural method is as follows:

Breeding water body: after nylon membrane, biological adsorption agent, active carbon, biochemical filter and UV germicidal system Pyatyi filtration treatment, salinity is 28-30, and dissolved oxygen levels is the seawater of 4-8mg/L

Temperature of cultivation: 26-30 degree Celsius

Cultivation density: vegetative period is 0.2-0.5L/ tail, breeding period is 0.5-1L/ tail

Photoperiod: illumination 14 hours, dark 10 hours, and lighting hours is 5:00-19:00

Cultivation sex ratio: male: female=2:3

Bait throwing in number of times and bait throwing in time: 7:30-8:30,11:30-12:30,15:30-16:30 of every day divide seawater feed for tropical fish of throwing something and feeding for three times, 9:45-10:15,13:45-14:15 of every day throw something and feed artemia larvae at twice.

Compared with the existing technology, tool has the following advantages in the present invention:

The laboratory anniversary cultural method of the blue or green Medaka of seawater proposed by the invention, has relatively strict cultivating condition, and lower cost simulation can be utilized to go out the self-sow environment of the blue or green Medaka of stain, is more conducive to growth and the breeding of the blue or green Medaka of stain; And profit cultivates the blue or green Medaka of stain in this way, can realize the integration of the processes such as raising, breeding and hatching, save time, simplify breeding process, its quantity of laying eggs and quality all increase, for scientific research provides stable guarantee; And this method is simple, be particularly suitable for ecological toxicology research in laboratory model organism cultivation in promote and application, its market prospects and significance of scientific research are all very good.

Embodiment

The specific embodiment of the present invention will be described in detail below.

The blue or green Medaka of a kind of seawater (the blue or green Medaka of stain, oryzias melastigma) laboratory anniversary cultural method, it is characterized in that: the cultivating condition of described cultural method is as follows:

Breeding water body: the seawater after nylon membrane, biological adsorption agent, active carbon, biochemical filter and UV germicidal system Pyatyi filtration treatment, controlling its salinity is 28-30, and dissolved oxygen levels is 4-8mg/L,

Temperature of cultivation: 26-30 degree Celsius,

Cultivation density: vegetative period is 0.2-0.5L/ tail, the blue or green Medaka of stain growing more than three months enters breeding period, and the cultivation density between breeding period is 0.5-1L/ tail,

Cultivation sex ratio: male: female=2:3

Every day carries out five bait throwing in the blue or green Medaka of stain, 7:30-8:30 is first time bait throwing in, 9:45-10:15 is second time bait throwing in, 11:30-12:30 is third time bait throwing in, 13:45-14:15 is the 4th bait throwing in, 15:30-16:30 is the 5th bait throwing in, and wherein the first, the 3rd and the 5th bait throwing in is thrown something and fed seawater feed for tropical fish, and first time bait throwing in and third time bait throwing in, time interval between third time bait throwing in and the 5th bait throwing in are four hours; The second, the 4th bait throwing in is thrown something and fed artemia larvae, and the time interval between second time bait throwing in and the 4th bait throwing in is similarly four hours; Each bait throwing in adopts a small amount of principle repeatedly, to the blue or green Medaka of stain is no longer ingested;

In order to assist to cultivate illumination, ensure that illumination is sufficient and even, opened by the illuminating lamp that cultivation is indoor at the 8:00 of every day, 17:00 closes illuminating lamp, and the illuminator only by breeding box provides light;

This Laboratory culture method, because water temperature is higher, evaporation of seawater amount is larger, every day, the salinity of breeding water body can slightly improve, therefore for ensureing the stability of salinity, the salinity of water body must be measured every day, when salinity is more than 30, under the condition that filtered water pump is opened, in water body, slowly add appropriate distilled water stablize to maintain salinity.

Under this method can realize the laboratory environment of the blue or green Medaka of seawater (the blue or green Medaka of stain, Oryzias melastigma), large-scale anniversary cultivation.

Claims

1. a laboratory anniversary cultural method for the blue or green Medaka of seawater, is characterized in that: the cultivating condition of described cultural method is as follows:

Temperature of cultivation: 26-30 degree Celsius

Cultivation sex ratio: male: female=2:3