CN104222024A - Method for artificial mass breeding of Paederus fuscipes Curtis - Google Patents
Method for artificial mass breeding of Paederus fuscipes Curtis Download PDFInfo
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Abstract
The invention relates to a method for artificial mass breeding of Paederus fuscipes Curtis. The method comprises the following steps of using rice, injurious insects of 1-3 aged Nilaparvata lugens nymphs or all-aged Nilaparvata lugens, and an artificial feed to breed Paederus fuscipes Curtis in the environment with a temperature of 28 DEG C and a relative humidity of 65%-80%, wherein the artificial feed is prepared by mixing honey with an animal liver powder feed, and the Paederus fuscipes Curtis adults spawn massively after breeding for 5 days; picking out Paederus fuscipes Curtis larvae, which are hatched every day, by a writing brush; breeding the larvae according to a Paederus fuscipes Curtis larva breeding method. After the Paederus fuscipes Curtis larvae are bred for 30 days, the larvae become adults through eclosion, and the adults are transferred into a glass container for breeding according to a Paederus fuscipes Curtis adult breeding method. The adults are bred in glass containers when the population density of natural enemy Paederus fuscipes Curtis imagoes is not less than 100, and the population density of Paederus fuscipes Curtis larvae is not less than 40. The method provided by the invention has the advantages of simplicity in operation, high breeding survival rate, low cost and capability of providing insect sources to a biosafety evaluation work which takes Paederus fuscipes Curtis as indicative insects.
Description
Technical field
The present invention relates to the method for the hidden wing first of the blue or green wing ant shape of a kind of artificial mass rearing.
Background technology
The utilization of transgenic anti-insect plants is the once huge green transformantion of crop pest Prevention Technique, at present, cry gene is the most widely used anti insect gene, come from a kind of Gram-positive Soil Bacillus---bacillus thuringiensis (Bacillus thuringiensis, be called for short Bt), belong to prokaryotes (Procaryotic organism) bacterium guiding principle (Bacteria) Bacillaceae (Bacillaceae) Bacillus (Bacillus).In sporulation process, the one or both ends of Bt thalline can be formed one or morely has bioactive protein crystal, this protein crystal is encoded by cry and cyt, virose albumen can be activated in some insect bodies, and be combined with the epithelial specific receptor of insect midgut, it is made to produce perforation, thus kill insects.Transgenic anti-insect plants is exactly the characteristic by research Bt bacterium cry and cyt, and is modified transform after proceed in plant and express, make plant have the ability of resisting special insect.The emergence and development of Bt genetically modified plants has made tremendous contribution for improving output, reducing production cost and reducing environmental pollution.
Transgenic anti-insect plants is while the mankind bring interests, also potential ecological risk may be caused to environment, therefore be necessary to select suitable instruction insect to carry out urban eco landscape forest, in recent years, insect-resistant transgenic crops is the focus that correlative study person pays close attention to non-target organism safety always.
But safety evaluation can not be carried out to each insect, select suitable Indicative Insect significant in safety evaluation work.At present, select non-target coleoptera predator mainly to concentrate on ladybug and ground beetle as Indicative Insect in genetically modified crops environmental safety assessment, and the hidden wing first of blue or green wing ant shape in Staphylinidae is seldom related to.Blue or green wing ant shape hidden wing first is large to insect predatory number, can effectively control the various pests such as leafhopper, rice fulgorid, thrips, cotton bollworm, and blue or green wing ant shape hidden wing first is large at field generating capacity, be dominant population in many areas, Bt is likely passed to the hidden wing first of blue or green wing ant shape by food chain by taking food genetically modified crops by plant-feed insect, adds the approach that this natural enemy is exposed to Bt albumen.But because the hidden wing first of blue or green wing ant shape raises difficulty greatly, especially larva is harsh to environmental requirement, this worm is therefore made to limit to some extent as instruction insect in environmental safety assessment.Thus, the method working out the hidden wing first of the blue or green wing ant shape of the artificial mass rearing of a kind of energy is needed.
Summary of the invention
The object of the invention is for above-mentioned present situation, aim to provide a kind of simple, efficient, cost is low, can provide the method for the hidden wing first of the blue or green wing ant shape of artificial mass rearing in worm source for the blue or green wing ant shape of genetically modified crops hidden wing first environmental safety assessment from now on.
The implementation of the object of the invention is, the method for the hidden wing first of the blue or green wing ant shape of artificial mass rearing, and concrete steps are as follows:
1) select to grow to more than 10cm rice seedlings for subsequent use;
2) prepare the prey brown planthopper nymph that 1-3 supplies blue or green wing ant shape hidden wing first larva raising age, do not limit the length of time for the prey brown planthopper of blue or green wing ant shape hidden wing first imago breeding;
3) be the artificial feed that the ratio of 4-6:1 mixes as the hidden wing first of blue or green wing ant shape with mass ratio using complete for Yi Wei function hepar siccatum and honey, be placed on the culture dish that diameter is less than 5cm;
4) clean up and raise container, raise box, prepare, according to raising the sponge or absorbent cotton that the specification of container carries out cutting out, to be layered on raising container bottom, to add clear water and make its moisturizing that fully absorbs water;
5) by moistening polytrophic, be suitable for the soil uniform fold of nursery in step 7) sponge or absorbent cotton on, do not exceed and raise the edge of container;
6) raising of the blue or green wing ant shape of natural enemy hidden wing first adult: by 200 ± 50 steps 1) rice seedlings clear water rinse the glass beaker putting into 100ml well, add the paddy rice culture fluid of aequum, glass beaker put into and raises container; Step 3) artificial feed that prepare are contained in little culture dish, and little culture dish is placed on raises in container; Access does not limit the length of time, for the brown planthopper of blue or green wing ant shape hidden wing first imago breeding, with nylon gauze and rubber band sealing; Temperature 28 DEG C, under the environment of relative moisture 65%-80%, access opposes blue or green wing ant shape hidden wing first adult 50 ± 10 previous day, and feed is changed weekly, keeps the skin wet; Initial access amount≤50 of each raising container of the blue or green wing ant shape of natural enemy hidden wing first, random quantity of supplementing brown planthopper, when close degree≤100 of the adult insect population of the blue or green wing ant shape of natural enemy hidden wing first, point raising container is raised;
After 5d, the hidden wing first of blue or green wing ant shape of raising in container is laid eggs in a large number, the sponge and rice seedling that have ovum grain are taken out, being placed on bottom is covered with in the uncovered raising box of moistening toilet paper, with the foraminate preservative film of bundle and rubber band sealing, the blue or green wing ant shape hidden wing first larva writing brush of hatching is chosen by every day, by pressing step 7) method raising;
7) raising of the blue or green wing ant shape of natural enemy hidden wing first larva: by step 3) artificial feed that prepare are contained in slide and are placed in uncovered raising container, raise container bottom and be covered with water-absorbing sponge or absorbent cotton, be coated with the soil being suitable for nursery, get several rice seedlings, rice root is encased with moistening cotton, be positioned over and raise in container, access step 2) 1-3 age is for the brown planthopper nymph of blue or green wing ant shape hidden wing first larva raising, put into uncovered raising box, temperature 28 DEG C, under the environment of relative moisture 65%-80%, the blue or green wing ant shape of the natural enemy hidden wing first larva of access hatching, the initial worm amount that connects is less than 40, with nylon gauze, prick the preservative film sealing in hole, feed every day is changed, the random brown planthopper supplementing the low length of time, and when the larva insect population of the blue or green wing ant shape of natural enemy hidden wing first close degree≤40, capacitance divider is raised,
Raise and the blue or green wing ant shape hidden wing first adult sprouted wings chosen, by step 6 in 15-30 days) method raising;
The long 22cm of described uncovered raising box, wide 7cm, high 7cm.
The present invention utilizes the hidden wing first of the blue or green wing ant shape of animal liver meal feed artificial mass rearing natural enemy of paddy rice and insect brown planthopper thereof, blending honey, simple to operate, raising survival rate is high, and cost is low, can in good time for the hidden wing first of blue or green wing ant shape in biological safety evaluation work provides worm source as instruction insect.
Embodiment
The present invention utilizes the hidden wing first of the blue or green wing ant shape of animal liver meal feed artificial mass rearing natural enemy of paddy rice and prey brown planthopper thereof, blending honey.The blue or green wing ant shape of described natural enemy hidden wing first refers to the hidden wing first of blue or green wing ant shape (Paederus fuscipes Curtis) in coleoptera (Coleoptera) Staphylinidae (Staphylinidae) Paederus (Paederus).Described prey brown planthopper refers to the brown planthopper (Nilaparvata lugens (Stal)) in Semiptera (Hemiptera) Delphacidae (Delphacidae).Concrete steps are as follows:
1) for subsequent use when selecting to grow to more than 10cm rice seedlings.Rice seedlings is bright extensive 63 strain rice seedlings, and rice paddy seed is provided by State Key Laboratory of Crop Genetic Improvent.Paddy rice is cultivated to more than 10cm with Yoshida culture fluid in dish for cultivating (length × wide × height=25cm × 20cm × 3cm).
2) prepare the brown planthopper nymph that 1-3 supplies blue or green wing ant shape hidden wing first larva raising age, do not limit the length of time for the brown planthopper of blue or green wing ant shape hidden wing first imago breeding.
3) be the artificial feed that the ratio of 4-6:1 mixes as the hidden wing first of blue or green wing ant shape with mass ratio using complete for commercial Yi Wei function hepar siccatum and honey, be placed on diameter and be less than on the culture dish of 5cm; The full function hepar siccatum of Yi Wei and honey are that the ratio of 5:1 mixes the best with mass ratio.
4) clean up raising container, according to raising sponge that the specification of container carries out cutting out or absorbent cotton is layered on raising container bottom, adding clear water and making its moisturizing that fully absorbs water, providing water source as the hidden wing first of the blue or green wing ant shape of natural enemy.
5) by moistening polytrophic, the soil being suitable for nursery pours the step 7 of printing opacity into) uncovered raising container in, do not exceed and raise the edge of container.Soil is excellent with horticultural crop seeding and seedling raising soil.
6) raising of the blue or green wing ant shape of natural enemy hidden wing first adult: by 200 ± 50 steps 1) rice seedlings clear water rinse the glass beaker putting into 100ml well, add the paddy rice culture fluid of aequum, glass beaker put into and raises container; Step 3) artificial feed that prepare are contained in little culture dish, and little culture dish is placed on raises in container; Access does not limit the length of time, for the brown planthopper of blue or green wing ant shape hidden wing first imago breeding, temperature 28 DEG C, under the environment of relative moisture 65%-80%, access opposes blue or green wing ant shape hidden wing first adult 50 ± 10 previous day, with 200 order nylon gauzes, the preservative film pricking hole, rubber band sealing, prevent brown planthopper, the hidden wing first of blue or green wing ant shape runs away.Feed is changed weekly, and keeps the skin wet to the glass beaker of sponge and placement paddy rice according to actual conditions; Initial access amount≤50 of each raising container of the blue or green wing ant shape of natural enemy hidden wing first, random quantity of supplementing brown planthopper, when close degree≤100 of the adult insect population of the blue or green wing ant shape of natural enemy hidden wing first, point raising container is raised;
After 5d, consider that the hidden wing first of blue or green wing ant shape is laid eggs in a large number, the sponge raised in container and rice seedlings are taken out and is placed on bottom, be paved with in the uncovered plastic casing of moistening toilet paper, also can replace toilet paper with absorbent cotton.With pricking the preservative film in hole and rubber band sealing, the blue or green wing ant shape hidden wing first ovum grain hatching on sponge and rice seedling, the blue or green wing ant shape hidden wing first larva writing brush of hatching is chosen by every day, by by step 7) method raises.
Described raising container is the glass beaker of 2000ml.
7) raising of the blue or green wing ant shape of natural enemy hidden wing first larva: by step 3) artificial feed that prepare are contained in slide and are placed on and raise in container, raise container bottom and be covered with water-absorbing sponge or absorbent cotton, be coated with the soil being suitable for nursery, get several rice seedlings, rice root is encased with moistening cotton, be positioned over and raise in container, access step 2) 1-3 age is for the brown planthopper nymph of blue or green wing ant shape hidden wing first larva raising, put into uncovered raising box, temperature 28 DEG C, under the environment of relative moisture 65%-80%, the blue or green wing ant shape of the natural enemy hidden wing first larva of access hatching, the initial worm amount that connects is no more than 40, with 200 order nylon gauzes, prick the preservative film in hole, rubber band seals, prevent brown planthopper, the hidden wing first of blue or green wing ant shape is run away, feed every day is changed, the random brown planthopper supplementing the low length of time, and when the larva insect population of the blue or green wing ant shape of natural enemy hidden wing first close degree≤40, capacitance divider is raised,
Raise and the blue or green wing ant shape hidden wing first adult sprouted wings chosen, by step 6 in 15-30 days) method raising;
The long 22cm of described uncovered raising box, wide 7cm, the plastic casing of high 7cm.
With specific embodiment in detail the present invention is described in detail below.
Example 1,
1) selection is provided by State Key Laboratory of Crop Genetic Improvent, and paddy rice is cultivated for subsequent use to the rice seedlings of more than 10cm with Yoshida culture fluid in dish for cultivating (length × wide × height=25cm × 20cm × 3cm);
2) prepare the prey brown planthopper nymph that 1-3 supplies blue or green wing ant shape hidden wing first larva raising age, do not limit the length of time for the prey brown planthopper of blue or green wing ant shape hidden wing first imago breeding;
3) be the artificial feed that the ratio of 5:1 mixes as the hidden wing first of blue or green wing ant shape with mass ratio using complete for Yi Wei function hepar siccatum and honey, be placed on the culture dish that diameter is less than 5cm;
4) glass beaker cleaning up 2000ml prepares, according to raising the sponge or absorbent cotton that the specification of container carries out cutting out, to be layered on raising container bottom, to add clear water and make its moisturizing that fully absorbs water; Abundant water suction moisturizing provides water source for the hidden wing first of the blue or green wing ant shape of natural enemy.
5) by horticultural crop seeding and seedling raising soil uniform fold in step 7) sponge on, do not exceed and raise the edge of container;
6) raising of the blue or green wing ant shape of natural enemy hidden wing first adult: by 150-190 root step 1) rice seedlings clear water rinse the glass beaker putting into 100ml well, add the paddy rice culture fluid of aequum, glass beaker put into the glass beaker of 2000ml; Step 3) artificial feed that prepare are contained in little culture dish, and little culture dish is placed on raises in container; Access does not limit the length of time in a large number, for the brown planthopper of blue or green wing ant shape hidden wing first imago breeding, runs away with nylon gauze, the preservative film pricking hole and rubber band sealing control; Temperature 28 DEG C, under the environment of relative moisture 65%-70%, access 40-45 opposes blue or green wing ant shape hidden wing first adult the previous day, and feed is changed weekly, and keeps the skin wet to the glass beaker of sponge and placement paddy rice according to actual conditions; Initial access amount≤50 of each raising container of the blue or green wing ant shape of natural enemy hidden wing first, random quantity of supplementing brown planthopper, when close degree≤100 of the adult insect population of the blue or green wing ant shape of natural enemy hidden wing first, point raising container is raised;
After 5d, consider that the hidden wing first of blue or green wing ant shape is laid eggs in a large number, the sponge raised in container and rice seedlings are taken out and puts into the raising box that bottom is paved with moistening toilet paper, the blue or green wing ant shape hidden wing first larva writing brush of hatching is chosen by every day, raises by the feeding manner of the blue or green wing ant shape of natural enemy hidden wing first larva.
7) raising of the blue or green wing ant shape of natural enemy hidden wing first larva: by step 3) artificial feed that prepare are contained in slide and are placed on the moistening sponge in paving, bottom and cover the long 22cm of substrate soil, wide 7cm, in the uncovered raising box of high 7cm, get several steps 1) rice seedlings, rice root is encased with moistening cotton, be positioned over and raise in container, access step 2) 1-3 age is for the brown planthopper nymph of blue or green wing ant shape hidden wing first larva raising, successively with 200 order nylon gauzes, the preservative film pricking hole and rubber band sealing, nylon wire prevents from runing away.Temperature 28 DEG C, under the environment of relative moisture 65%-70%, the blue or green wing ant shape of the natural enemy hidden wing first larva of access hatching, the initial worm amount that connects is less than 40; Feed every day is changed, the random brown planthopper supplementing the low length of time, and when the larva insect population of the blue or green wing ant shape of natural enemy hidden wing first close degree≤40, capacitance divider is raised;
Raise and the blue or green wing ant shape hidden wing first adult sprouted wings was chosen in 15-30 days, raise according to the feeding manner of the blue or green wing ant shape of natural enemy hidden wing first adult.
Example 2, with example 1, unlike,
3) using complete for Yi Wei function hepar siccatum and honey with mass ratio be the artificial feed that the ratio of 4:1 mixes as the hidden wing first of blue or green wing ant shape.
6) by 190-210 root step 1) rice seedlings clear water rinse the glass beaker putting into 100ml well.Temperature 28 DEG C, under the environment of relative moisture 70-77%, access about 48-54 and oppose blue or green wing ant shape hidden wing first adult the previous day.
7) the brown planthopper nymph for blue or green wing ant shape hidden wing first larva raising puts into uncovered raising box, temperature 28 DEG C, under the environment of relative moisture 70-77%, and the blue or green wing ant shape of the natural enemy hidden wing first larva of access hatching.
Example 3,
3) using complete for Yi Wei function hepar siccatum and honey with mass ratio be the artificial feed that the ratio of 6:1 mixes as the hidden wing first of blue or green wing ant shape
6) by 210-250 root step 1) rice seedlings clear water rinse the glass beaker putting into 100ml well.At temperature 78-80%, under the environment of relative moisture 70%, access about 52-60 and oppose blue or green wing ant shape hidden wing first adult the previous day.
7) the brown planthopper nymph for blue or green wing ant shape hidden wing first larva raising puts into uncovered raising box, temperature 28 DEG C, under the environment of relative moisture 78-80%, and the blue or green wing ant shape of the natural enemy hidden wing first larva of access hatching.
Claims (5)
1. the method for the hidden wing first of the blue or green wing ant shape of artificial mass rearing, is characterized in that concrete steps are as follows:
1) select to grow to more than 10cm rice seedlings for subsequent use;
2) prepare the prey brown planthopper nymph that 1-3 supplies blue or green wing ant shape hidden wing first larva raising age, do not limit the length of time for the prey brown planthopper of blue or green wing ant shape hidden wing first imago breeding;
3) be the artificial feed that the ratio of 4-6:1 mixes as the hidden wing first of blue or green wing ant shape with mass ratio using complete for Yi Wei function hepar siccatum and honey, be placed on the culture dish that diameter is less than 5cm;
4) clean up and raise container, raise box, prepare, according to raising the sponge or absorbent cotton that the specification of container carries out cutting out, to be layered on raising container bottom, to add clear water and make its moisturizing that fully absorbs water;
5) by moistening polytrophic, be suitable for the soil uniform fold of nursery in step 7) sponge or absorbent cotton on, do not exceed and raise the edge of container;
6) raising of the blue or green wing ant shape of natural enemy hidden wing first adult: by 200 ± 50 steps 1) rice seedlings clear water rinse the glass beaker putting into 100ml well, add the paddy rice culture fluid of aequum, glass beaker put into and raises container; Step 3) artificial feed that prepare are contained in little culture dish, and little culture dish is placed on raises in container; Access does not limit the length of time, for the brown planthopper of blue or green wing ant shape hidden wing first imago breeding, with nylon gauze, the preservative film sealing of pricking hole; Temperature 28 DEG C, under the environment of relative moisture 65%-80%, access opposes blue or green wing ant shape hidden wing first adult 50 ± 10 previous day, and feed is changed weekly, keeps the skin wet; Initial access amount≤50 of each raising container of the blue or green wing ant shape of natural enemy hidden wing first, random quantity of supplementing brown planthopper, when close degree≤100 of the adult insect population of the blue or green wing ant shape of natural enemy hidden wing first, point raising container is raised;
After 5d, the hidden wing first of blue or green wing ant shape of raising in container is laid eggs in a large number, the sponge and rice seedling that have ovum grain are taken out, be placed on and be covered with in the uncovered raising box of moistening toilet paper, with the foraminate preservative film of bundle and rubber band sealing, the blue or green wing ant shape hidden wing first larva writing brush of hatching is chosen by every day, by pressing step 7) method raising;
7) raising of the blue or green wing ant shape of natural enemy hidden wing first larva: by step 3) artificial feed that prepare are contained in slide and are placed in uncovered raising container, raise container bottom and be covered with water-absorbing sponge or absorbent cotton, be coated with the soil being suitable for nursery, get several rice seedlings, rice root is encased with moistening cotton, be positioned over and raise in container, access step 2) 1-3 age is for the brown planthopper nymph of blue or green wing ant shape hidden wing first larva raising, temperature 28 DEG C, under the environment of relative moisture 65%-80%, the blue or green wing ant shape of the natural enemy hidden wing first larva of access hatching, the initial worm amount that connects is less than 40, with nylon gauze, prick the preservative film sealing in hole, feed every day is changed, the random brown planthopper supplementing the low length of time, and when the larva insect population of the blue or green wing ant shape of natural enemy hidden wing first close degree≤40, capacitance divider is raised,
Raise and the blue or green wing ant shape hidden wing first adult sprouted wings chosen, by step 6 in 15-30 days) method raising;
The long 22cm of described uncovered raising box, wide 7cm, high 7cm.
2. the method for the hidden wing first of the blue or green wing ant shape of artificial mass rearing according to claim 1, it is characterized in that rice seedlings is bright extensive 63 strain rice seedlings, paddy rice is cultivated to more than 10cm with Yoshida culture fluid in length × wide × height=25cm × 20cm × 3cm dish for cultivating.
3. the method for the hidden wing first of the blue or green wing ant shape of artificial mass rearing according to claim 1, is characterized in that the full function hepar siccatum of Yi Wei and honey mix with the ratio of mass ratio 5:1.
4. the method for the hidden wing first of the blue or green wing ant shape of artificial mass rearing according to claim 1, is characterized in that step 5) soil be with the soil of horticultural crop seeding and seedling raising.
5. the method for the hidden wing first of the blue or green wing ant shape of artificial mass rearing according to claim 1, after it is characterized in that 5d, the sponge raised in container and rice seedlings are taken out and is paved with in the uncovered plastic casing of moistening toilet paper bottom being placed on, with 200 order nylon gauzes, the preservative film pricking hole and rubber band sealing.
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| CN107950489A (en) * | 2017-12-22 | 2018-04-24 | 江西省农业科学院植物保护研究所 | A kind of method of indoor feeding green grass or young crops wing ant shape rove beetle |
| CN109892290A (en) * | 2018-05-29 | 2019-06-18 | 华中农业大学 | A kind of preparation of cotton nitidulid man-made feeds and the method raised with it |
| CN110951730A (en) * | 2019-11-04 | 2020-04-03 | 华中农业大学 | dsRNA of cryptopteris viridis V-ATPase-A gene, artificial feed and application thereof |
| CN115152705A (en) * | 2022-08-11 | 2022-10-11 | 湖南省水稻研究所 | Method and device for feeding rice planthoppers |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115152705A (en) * | 2022-08-11 | 2022-10-11 | 湖南省水稻研究所 | Method and device for feeding rice planthoppers |
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| CN104222024B (en) | 2016-06-08 |
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