CN106616070A - Chestnut weevil artificial feed, cultivation method and application of mature larvae - Google Patents

Chestnut weevil artificial feed, cultivation method and application of mature larvae Download PDF

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Publication number
CN106616070A
CN106616070A CN201610831928.3A CN201610831928A CN106616070A CN 106616070 A CN106616070 A CN 106616070A CN 201610831928 A CN201610831928 A CN 201610831928A CN 106616070 A CN106616070 A CN 106616070A
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days
feed
chinese chestnut
larva
chestnut
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CN106616070B (en
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吴国星
秦小萍
吴道慧
高熹
何霞红
李强
兰明先
郝凯强
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Yunnan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention provides a chestnut weevil artificial feed, cultivation method and application of mature larvae. The feed contains peanuts, wheat, soybean, corn starch, sugar, multivitamin and carotene. The cultivation method comprises the following steps of: putting pubescent male and female chestnut weevils, fresh chestnut tender leaves and a feed into a feeding box, performing cultivation under certain conditions for 3-5 days, putting chestnut young fruits with flowers and young fruits of other quercus species, and performing cultivation under certain conditions for 6-8 hours so as to ensure that chestnut weevils perform natural spawning; further cultivating the young fruits subjected to spawning for 5-6 days, stripping off peels, transferring larvae into a feeding box with a feed, and performing cultivation under certain conditions for 35-40 days so as to obtain mature larvae; and further inducing nymphosis of the mature larvae. The obtained mature larvae or pupae can be used as cultivation hosts of scleroderma guani xiao et Wu and other parasitic wasps or breeding materials for later use. The chestnut weevils are used for artificial breeding for the first time, the method is simple, feed ingredients are easy to obtain, and magnified breeding is easy to perform.

Description

The purposes of Chinese chestnut weevil man-made feeds, cultural method and mature larva or pupa
Technical field
The invention belongs to insect technique field, and in particular to propagate Chinese chestnut weevil artificially.
Background technology
Chinese chestnut weevil (Curculio davidi Fairmaire) belongs to coleoptera, Curculionidae, also referred to as curculio davidi, plate Chestnut weevil, is distributed in Chinese each Chinese chestnut producing region, is caused harm chestnut reality with larva, and chestnut reality percentage of injury is up to more than 80%.Patent It is found by the applicant that but undisclosed:Scleroderma Xiao et Wu (Scleroderma guani Xiao et Wu) can be right under artificial feeding environment Chinese chestnut weevil is efficiently parasitic, and this is caused to be carried out by manual intervention, using Scleroderma Xiao et Wu the preventing and treating of Chinese chestnut weevil and becomes one kind has The biological control means of great potential.The preventing and treating of Chinese chestnut weevil is carried out with Scleroderma Xiao et Wu needs artificial breeding and the swollen legs of rejuvenation Guan Shi Honeybee, in this course, patent applicant has found but undisclosed again:Chinese chestnut weevil larva and pupa to Scleroderma Xiao et Wu breed and Rejuvenation has positive facilitation.Additionally, Chinese chestnut weevil larva is rich in protein, edible is gone back.
The content of the invention
It is an object of the invention to provide the purposes of Chinese chestnut weevil man-made feeds, cultural method and mature larva or pupa.
The present invention propagates the man-made feeds of Chinese chestnut weevil artificially:
By weight, 80~100 parts of peanut powder, 550~600 parts of wheat flour, 75~100 parts of the soy meal for cooking, corn 30~50 parts of 50~80 parts of powder, glucose or sucrose, 0.14~0.16 part of vitamin A, 0.09~0.11 part of vitamin C, class 1.9~2.1 parts of carrotene, 0.009~0.011 part of vitamin B1,0.009~0.011 part of vitamin B2, vitamin B3 0.04~0.06 part, 400 parts of water makes 110~130 mesh fine graineds after mixing well.The feed is refrigerated at 0~4 DEG C and can preserve 20 ~30 days, recover to normal temperature when using.
Cultural method of the present invention is:
Sexually matured Chinese chestnut weevil worm is pressed into male and female quantity 2:3 ratio, in being placed on the raising box for adding cover strip pore, box In place fresh Chinese chestnut tender leaf and above-mentioned man-made feeds simultaneously, tender leaf placement amount is that every adult is daily 9~11 grams, and feed is placed Amount is that every adult is daily 0.4~0.6 gram, and a tender leaf and feed are changed daily, strong in relative humidity 40%~70%, illumination After cultivating 3~5 days under conditions of degree 100lux~500lux, hour/day of light application time 12~16,20~35 DEG C of temperature, it is put into Wounded in the battle Chinese chestnut young fruit or oak belongs to the young fruit of other kinds, and adjustment intensity of illumination is 2~8lux, temperature is 22~26 DEG C, and cultivation 6~ 8 hours, Chinese chestnut weevil can natural spawning;Continue to cultivate the young fruit 5~6 for having been produced ovum in the case where above-mentioned breeding condition is constant My god, strip off pericarp is transferred to larva in the raising box that placed above-mentioned man-made feeds, and feed injected volume is that every larva is every Secondary 0.15~0.25 gram, two days change a feed, environment be 5~10lux of intensity of illumination, 22~35 DEG C of temperature, relative humidity 40%~60%, cultivation can become mature larva in 35~40 days;If subsequently increasing intensity of illumination to 100lux~200lux, light It is 8~12 hours/day according to the time, relative humidity is reduced to 29~31%, temperature-resistant, can further induce through 6~10 days Mature larva nymphosis.Depending on larvae development situation, addition in 10~15 days before nymphosis feeds the Specific amounts that man-made feeds feed The fresh rice-chestnut powder of 20% weight can promote nymphosis.
Purposes of the present invention is:
The Chinese chestnut weevil mature larva obtained by the inventive method or pupa are refrigerated 200~300 days in 2~4 DEG C, as pipe The swollen leg honeybee of family name and other parasitic wasp cultivation hosts use.
The Chinese chestnut weevil pupa absorbent cotton obtained by the inventive method parcel or fertile soil embedding is cold after 10~15 DEG C Hide 150 days~220 days, it is stand-by as material is bred, can turn into adult through 10~20 days at 18~25 DEG C using front.
Beneficial effects of the present invention:First Chinese chestnut weevil is carried out into artificial breeding, method is simple, feed ingredient is easily obtained, Easily being enlarged is bred.
Specific embodiment
Embodiment 1:Sexually matured Chinese chestnut weevil adult is pressed into female-male proportion 2:3 ratio, is placed into and adds cover strip pore In transparent raising box, the amount in box every time according to every cephalont 11g Chinese chestnuts fresh leaf and 0.4g man-made feeds is placed, and is changed once daily Fresh leaf and feed.Breeding environment is:Humidity 40%, intensity of illumination is 100lux, and light application time is 12 hours/day, 35 DEG C of temperature Under the conditions of after cultivation 3 days, be placed into spawning with wounded in the battle Chinese chestnut young fruit branch, adjustment intensity of illumination 2lux, temperature control is 22 DEG C, cultivate 6 hours, after checking spawning situation, branch is taken out in transparent raising box, cultivate in the case that environmental condition is constant 5 days, period ensured the fresh and alive property of branch.Careful strip off pericarp, larva is transferred in artificial feeding box after 5 days, is in environment: Humidity 40%, whole day intensity of illumination is 10lux, is cultivated under the conditions of 22 DEG C of temperature 35 days, larva is shifted afterwards and is to environment:Humidity 31%, intensity of illumination is 100lux, and light application time is 12 hours/day, is picked after cultivating 6 days under the conditions of 22 DEG C of temperature except nymphosis Larva, from the beginning of being transferred to artificial feeding box from larva, every time every larva feeds man-made feeds 0.15g, changes a people within two days Work feed.Pupa strongr after nymphosis is individually sorted out as breeding and reserved seed for planting use, at 2 DEG C of remaining pupa standby, cold preservation time is refrigerated For 300 days, take out the host for recovering to normal temperature to be used for Scleroderma Xiao et Wu and use, parasitic effects are good.The breeding rotten plant of pupa Refrigerate at 10 DEG C after native embedding, can preserve 220 days, sprout wings naturally within two weeks at 18 DEG C during hatching and be unearthed, with tender Chinese chestnut leaf and manually Feed mixing is raised to sexal maturity.This man-made feeds for using is:Peanut powder 80g;Wheat flour 550g;The soybean for cooking Powder 75g;Corn flour 50g;Glucose or sucrose 30g;Vitamin A:140mg;Vitamin C:90mg;Carotenoid 1.9g;Dimension life Plain B1:9mg;Vitamin B2:9mg;Vitamin B3:40mg;Add water 400ml, and 110 mesh fine graineds are made after mixing well, you can.Refrigeration Can preserve at 0 DEG C 30 days, recover to normal temperature when using.
Embodiment 2:Sexually matured Chinese chestnut weevil adult is pressed into female-male proportion 2:3 ratio, is placed into and adds cover strip pore In transparent raising box, the amount in box every time according to every cephalont 9g Chinese chestnuts fresh leaf 0.5g man-made feeds is placed and changed daily once Fresh leaf and feed.Breeding environment is:Humidity 70%, intensity of illumination is 500lux, and light application time is 16 hours/day, 35 DEG C of temperature Under the conditions of after cultivation 5 days, be placed into spawning with wounded in the battle Chinese chestnut young fruit branch, adjustment intensity of illumination 8lux, temperature control is 24 DEG C, cultivate 8 hours, after checking spawning situation, branch is taken out in transparent raising box, cultivate in the case that environmental condition is constant 6 days, period ensured the fresh and alive property of branch.Careful strip off pericarp, larva is transferred in artificial feeding box after 6 days, is in environment: Humidity 60%, whole day intensity of illumination is 5lux, is cultivated under the conditions of 22 DEG C of temperature 40 days, larva is shifted afterwards and is to environment:Humidity 29%, intensity of illumination is 200lux, and light application time is 12 hours/day, picks after cultivating 10 days under the conditions of 22 DEG C of temperature and removes nymphosis Larva, be transferred to before artificial feeding box starts to nymphosis to complete from larva, every time every larva feeds man-made feeds 0.25g, A feed is changed within two days, until nymphosis is completed.Pupa strongr after nymphosis is individually sorted out as breeding and reserved seed for planting use, remaining pupa Refrigerate at 4 DEG C it is standby, cold preservation time be 200 days, take out recover to normal temperature be used for Scleroderma Xiao et Wu host use, parasitism effect Fruit is good.Breeding pupa can be preserved 150 days with refrigerating at 15 DEG C after peat embedding, be sprouted wings naturally within 10 days at 25 DEG C during hatching It is unearthed, is raised to sexal maturity with tender Chinese chestnut leaf and man-made feeds mixing.This man-made feeds for using is:Peanut:100g; Wheat flour 600g;The soy meal 100g for cooking;Corn flour 80g;Glucose or sucrose 50g;Vitamin A:160mg;Vitamin C: 100mg;Carotenoid 2.1g;Vitamin B1:11mg;Vitamin B2:11mg;Vitamin B3:60mg;Add water 400ml, mixes well After make 130 mesh fine graineds, you can.Refrigeration can be preserved 20 days at 4 DEG C, be recovered to normal temperature when using.
Embodiment 3:Sexually matured Chinese chestnut weevil adult is pressed into female-male proportion 2:3 ratio, is placed into and adds cover strip pore In transparent raising box, the amount in box every time according to every cephalont 11g Chinese chestnuts fresh leaf and 0.5g man-made feeds is placed, and changes one daily Secondary fresh leaf and feed.Breeding environment is:Humidity 40%, intensity of illumination is 500lux, and light application time is 12 hours/day, temperature 20 After cultivating 3 days under the conditions of DEG C, the wounded in the battle Chinese chestnut young fruit branch of spawning is placed into, adjusts intensity of illumination 2lux, temperature control exists 26 DEG C, cultivate 8 hours, after checking spawning situation, branch is taken out in transparent raising box, train in the case that environmental condition is constant Support 5 days, period ensures the fresh and alive property of branch.Careful strip off pericarp, larva is transferred in artificial feeding box, in environment after 5 days For:Humidity 60%, whole day intensity of illumination is 10lux, is cultivated under the conditions of 35 DEG C of temperature 35 days, larva is shifted afterwards and is to environment:It is wet Degree 30%, intensity of illumination is 100lux, and light application time is 8 hours/day, picks after cultivating 8 days under the conditions of 35 DEG C of temperature and removes nymphosis Larva, be transferred to before artificial feeding box starts to nymphosis to complete from larva, every larva feeds every time man-made feeds 0.25g, Change a feed within two days, cultivating to the 32nd day every larva increases every time the fresh rice-chestnut powders of 0.05g, until nymphosis is completed.By pupa Strongr pupa is individually sorted out and reserved seed for planting use as breeding after change, refrigerates standby at 2 DEG C of remaining pupa, and cold preservation time is 300 days, is taken out Recover to normal temperature to be used for the host of Scleroderma Xiao et Wu, parasitic effects are good.Breeding pupa is with 15 DEG C after peat embedding Lower refrigeration, can preserve 220 days, sprout wings naturally within 20 days at 18 DEG C during hatching and be unearthed, and be raised with tender Chinese chestnut leaf and man-made feeds mixing To sexal maturity.This man-made feeds for using is:Peanut powder 80g;Wheat flour 600g;The soy meal 75g for cooking;Corn flour 80g;Glucose or sucrose 50g;Vitamin A:140mg;Vitamin C:110mg;Carotenoid 2.1g;Vitamin B1:10mg; Vitamin B2:9mg;Vitamin B3:50mg;Add water 400ml, and 120 mesh fine graineds are made after mixing well, you can.Refrigeration can at 0 DEG C Preserve 30 days, recover to normal temperature when using.
Embodiment 4:Sexually matured Chinese chestnut weevil adult is pressed into female-male proportion 2:3 ratio, is placed into and adds cover strip pore In transparent raising box, the amount in box daily according to every cephalont 9g Chinese chestnuts fresh leaf 0.4g man-made feeds is placed, and is changed daily once Fresh leaf and feed.Breeding environment is:Humidity 70%, intensity of illumination is 100lux, and light application time is 16 hours/day, 20 DEG C of temperature Under the conditions of after cultivation 5 days, be placed into spawning with wounded in the battle Chinese chestnut young fruit branch, adjustment intensity of illumination 8lux, temperature control is 22 DEG C, cultivate 8 hours, after checking spawning situation, branch is taken out in transparent raising box, cultivate in the case that environmental condition is constant 5 days, period ensured the fresh and alive property of branch.Careful strip off pericarp, larva is transferred in artificial feeding box after 5 days, is in environment: Humidity 60%, whole day intensity of illumination is 10lux, is cultivated under the conditions of 35 DEG C of temperature 35 days, larva is shifted afterwards and is to environment:Humidity 29%, intensity of illumination is 100lux, and light application time is 8 hours/day, is picked after cultivating 8 days under the conditions of 35 DEG C of temperature except nymphosis Larva, from larva be transferred to artificial feeding box start to nymphosis to complete before, every time every larva feed feed 0.15g, two days are more A feed is changed, cultivating to the 30th day every larva increases every time the fresh rice-chestnut powders of 0.03g, until nymphosis is completed.By after nymphosis compared with Strong pupa is individually sorted out and reserved seed for planting use as breeding, refrigerates standby at 2 DEG C of remaining pupa, and cold preservation time is 300 days, takes out and recovers extremely The host for being used for Scleroderma Xiao et Wu after normal temperature uses, and parasitic effects are good.The refrigeration at 10 DEG C after peat embedding of breeding pupa, Can preserve 150 days, sprout wings naturally within 10 days at 18 DEG C during hatching and be unearthed, be raised to sexal maturity with tender Chinese chestnut leaf and man-made feeds mixing .This man-made feeds for using is:Peanut powder 80g;Wheat flour 600g;The soy meal 75g for cooking;Corn flour 80g;Grape Sugar or sucrose 30g;Vitamin A:140mg;Vitamin C:110mg;Carotenoid 2g;Vitamin B1:11mg;Vitamin B2: 11mg;Vitamin B3:60mg;Add water 400ml, and 120 mesh fine graineds are made after mixing well, you can.Refrigeration can preserve 30 at 0 DEG C My god, recover to normal temperature when using.
Embodiment 5:Sexually matured Chinese chestnut weevil adult is pressed into female-male proportion 2:3 ratio, is placed into and adds cover strip pore In transparent raising box, place according to the amount of every cephalont 9g Chinese chestnuts fresh leaf 0.5g man-made feeds daily in box and daily replacing fresh leaf and Feed.Breeding environment is:Humidity 40%, intensity of illumination is 500lux, and light application time is 12 hours/day, under the conditions of 35 DEG C of temperature After cultivation 5 days, the wounded in the battle Chinese chestnut young fruit branch of spawning is placed into, adjusts intensity of illumination 2lux, temperature control is cultivated at 22 DEG C 8 hours, after checking spawning situation, branch is taken out in transparent raising box, cultivated 6 days in the case that environmental condition is constant, the phase Between ensure branch fresh and alive property.Careful strip off pericarp, larva is transferred in artificial feeding box after 6 days, is humidity in environment 40%, whole day intensity of illumination is 10lux, cultivation 40 days under the conditions of 22 DEG C of temperature, from the beginning of being transferred to artificial feeding box from larva, often Secondary every larva feeds man-made feeds 0.15g, changes a man-made feeds within two days.Sort out and refrigerated at 2 DEG C of larva, cold preservation time is 300 days, take out the host for recovering to normal temperature to be used for Scleroderma Xiao et Wu and use, parasitic effects are good.This artificial feeding for using Expect be:Peanut powder 80g;Wheat flour 550g;The soy meal 75g for cooking;Corn flour 50g;Glucose or sucrose 30g;Vitamin A: 150mg;Vitamin C:100mg;Carotenoid 2g;Vitamin B1:10mg;Vitamin B2:10mg;Vitamin B3:50mg;Plus Water 400ml, makes 120 mesh fine graineds after mixing well, you can.Refrigeration can be preserved 30 days at 4 DEG C, be recovered when using to normal temperature i.e. Can.

Claims (3)

1. a kind of Chinese chestnut weevil man-made feeds, it is characterised in that:By weight, 80~100 parts of peanut powder, wheat flour 550~600 30~50 parts of part, 75~100 parts of the soy meal for cooking, 50~80 parts of corn flour, glucose or sucrose, vitamin A 0.14~ 0.16 part, 0.09~0.11 part of vitamin C, 1.9~2.1 parts of carotenoid, 0.009~0.011 part of vitamin B1, dimension life 0.009~0.011 part of plain B2,0.04~0.06 part of vitamin B3,400 parts of water makes 110~130 mesh fine graineds after mixing well.
2. using the Chinese chestnut weevil cultural method of feed described in claim 1, it is characterised in that:,
Sexually matured Chinese chestnut weevil worm is pressed into male and female quantity 2:3 ratio, in being placed on the transparent raising box for adding cover strip pore, box In place fresh Chinese chestnut tender leaf and the feed simultaneously, tender leaf placement amount is that every adult is daily 9~11 grams, and feed placement amount is Every adult is daily 0.4~0.6 gram, a tender leaf and feed is changed daily, in relative humidity 40%~70%, intensity of illumination After cultivating 3~5 days under conditions of 100lux~500lux, hour/day of light application time 12~16,20~35 DEG C of temperature, band is put into Colored Chinese chestnut young fruit or oak belongs to the young fruit of other kinds, and adjustment intensity of illumination is 2~8lux, temperature is 22~26 DEG C, cultivates 6~8 Hour, Chinese chestnut weevil worm can natural spawning;Young fruit 5 after continuation culture is laid eggs in the case where above-mentioned breeding condition is constant~ 6 days, strip off pericarp was transferred to larva in the raising box that placed the feed, and feed injected volume is that every larva is each 0.15~0.25 gram, two days change a feed, environment be 5~10lux of intensity of illumination, 22~35 DEG C of temperature, relative humidity 40%~60%, cultivation can become mature larva in 35~40 days;Subsequently increase intensity of illumination to 100lux~200lux, illumination Time is 8~12 hours/day, and relative humidity is reduced to 29~31%, temperature-resistant, can further induce old through 6~10 days Ripe larva nymphosis.
3. the Chinese chestnut weevil mature larva for obtaining by claim 2 or the purposes of pupa, it is characterised in that:By mature larva or pupa in 2~4 DEG C refrigerate 200~300 days, use as Scleroderma Xiao et Wu and other parasitic wasps cultivation hosts, or by pupa absorbent cotton Parcel or fertile soil embedding are refrigerated 150 days~220 days after 10~15 DEG C, stand-by as material is bred, using front in 18~25 Adult can be turned at DEG C through 10~20 days.
CN201610831928.3A 2016-09-19 2016-09-19 Artificial feed for Chinese chestnut weevil, breeding method and application of aged larva or pupa Expired - Fee Related CN106616070B (en)

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CN109042545A (en) * 2018-09-21 2018-12-21 徐丽丽 A kind of weevil cultural method
CN110558457A (en) * 2019-08-30 2019-12-13 广西壮族自治区农业科学院 artificial feed for sweet potato elephant beetles and preparation method thereof
CN116250510A (en) * 2023-04-26 2023-06-13 伊犁哈萨克自治州林业科学研究院 Artificial rearing method for leg-swelling bees

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042545A (en) * 2018-09-21 2018-12-21 徐丽丽 A kind of weevil cultural method
CN109042545B (en) * 2018-09-21 2021-05-04 徐丽丽 Weevil breeding method
CN110558457A (en) * 2019-08-30 2019-12-13 广西壮族自治区农业科学院 artificial feed for sweet potato elephant beetles and preparation method thereof
CN116250510A (en) * 2023-04-26 2023-06-13 伊犁哈萨克自治州林业科学研究院 Artificial rearing method for leg-swelling bees
CN116250510B (en) * 2023-04-26 2024-06-04 伊犁哈萨克自治州林业科学研究院 Artificial rearing method for leg-swelling bees

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