CN103947879B - A kind of tea geometrid feed and preparation method thereof - Google Patents
A kind of tea geometrid feed and preparation method thereof Download PDFInfo
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- CN103947879B CN103947879B CN201410174219.3A CN201410174219A CN103947879B CN 103947879 B CN103947879 B CN 103947879B CN 201410174219 A CN201410174219 A CN 201410174219A CN 103947879 B CN103947879 B CN 103947879B
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Abstract
The invention discloses a kind of tea geometrid feed and preparation method thereof, relate to artificial feeding insect production field.The preparation method of tea geometrid feed of the present invention is: beer yeast powder is dissolved in distilled water by (1), then add stem tea powder successively, sucrose, glucose, Choline Chloride, methyl p-hydroxybenzoate, Webster salt stirs, and then add vitamin C, vitamin E, Cobastab mixed liquor, mixing and stirring; (2) agar powder is dissolved in hot water boiling rises 2-3 minute, then be poured in the mixture solution described in (1), the culture dish got ready is poured rapidly into after stirring, rocking culture dish makes feed bedding bottom culture dish even, strip is divided into pocket knife, obtained tea geometrid feed after agar solidification.The preparation method of tea geometrid feed of the present invention is simple, cost is low, and normal condition drops into feed next time can feed 3-5 days.
Description
Technical field
The present invention relates to artificial feeding insect production field, in particular a kind of tea geometrid feed and preparation method thereof.
Background technology
Tea geometrid (Ectropis obliqua Prout) is one of primary pest on tea tree.The phase hazard area of breaking out can reach 95% of the tea place gross area, and the lighter subtracts 5%, and severe one tea tree becomes polished rod.There is 5-6 generation in 1 year in Xinyang in tea geometrid, light is gnawed in full wafer tea place by large generation often, and shape, as burnt, affects greatly Tea Production.Although the pathogenic natural enemy of looper has a variety of, tea-geometrid-type polyhedrosis viruses (NPV) is that current research and application prospect are a kind of preferably.Tea geometrid polyhedrosis virus host specificity is strong, and environment compatibility is good.In addition, tea-geometrid-type polyhedrosis viruses preparation produce easy, easy to use, with low cost, be suitable for promote.
The artificial feeding of tea geometrid is the core prerequisite of this technology, and first the preparation of polyhedrosis virus must solve the artificial mass rearing problem of tea geometrid.Natural feed is subject to the restriction of the factors such as season, is therefore necessary the technique study of the raising carrying out man-made feeds and man-made feeds.High being not easy to of some feed toxigenic capacity is in the past promoted, and cannot continue generation cultivation, is difficult to meet the needs to worm source.
Summary of the invention
The present invention is high in order to solve tea geometrid feed cost, and the problems such as raising scale is little, provide a kind of tea geometrid feed and preparation method thereof.
Technical scheme of the present invention is as follows:
A preparation method for tea geometrid feed, its step is as follows:
(1) beer yeast powder is dissolved in distilled water, then add stem tea powder successively, sucrose, glucose, Choline Chloride, methyl p-hydroxybenzoate, Webster salt stirs, and then add vitamin C, vitamin E, Cobastab mixed liquor, mixing and stirring;
(2) agar powder is dissolved in hot water boiling rises 2-3 minute, then be poured in the mixture solution described in (1), the culture dish got ready is poured rapidly into after stirring, rocking culture dish makes feed bedding bottom culture dish even, after agar solidification, be divided into strip with pocket knife.
The preparation method of described Cobastab mixed liquor is, p-to 0.1g folic acid, 0.05g core yellow acid and 0.05g amido benzoic acid is put into small beaker, add 5-10ml distilled water to stir, add the potassium hydroxide solution that 1-2 drips 1N, be stirred to solution transparent, add 0.1g nicotinic acid again, 0.05g puridoxine hydrochloride, 0.1g biotin be made into Cobastab mixed liquor, put into refrigerator for subsequent use.
Described preparation method, calculate by weight, beer yeast powder 5 parts-10 parts, stem tea powder 20-30 part, sucrose 3-10 part, glucose 2.5 parts-5 parts, Choline Chloride 0.05-0.1 part, methyl p-hydroxybenzoate 0.2 part-0.5 part, Webster salt 0.5-2 part, vitamin C 0.1 part-1 part, vitamin E 0.03 part-0.08 part, Cobastab mixed liquor 0.006 part-0.01 part, agar powder 3-6 part, water 70-120 part.
The tea geometrid feed that described preparation method obtains.
Main protein source in tea geometrid feed of the present invention is beer yeast powder, and it belongs to the byproduct of Beer Brewage, and high temperature drying loses activity, and nutrient composition content is high, and cheap, wide material sources, easily deposits and easily puts; Containing energy growth promoting effects during the present invention is raw materials used, strengthen the anti-adversity ability of insect, survival rate is high, and this formula can not only raise insect the present age, but also the continuous generation that may be used for tea geometrid raises; The preparation method of tea geometrid feed of the present invention is simple, cost is low, and normal condition drops into feed next time can feed 3-5 days.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1 (man-made feeds I)
1, getting 6g brewer's yeast is dissolved in 40g distilled water, then add 15g stem tea powder successively, 4g sucrose, 3g glucose, 0.1g Choline Chloride, 0.2g methyl p-hydroxybenzoate, 1g Webster salt stirs, and then add 0.5g vitamin C, 0.05g vitamin E, 0.008g Cobastab mixed liquor, mix.
2, agar powder is dissolved in 80ml hot water boiling and rises 2-3 minute, then be poured in mixture solution described in 1, the culture dish got ready is poured rapidly into after stirring, rocking culture dish makes feed bedding bottom culture dish even, after agar solidification with pocket knife be divided into strip drop into raise tea geometrid crisper inside upper strata gauze on feed.
Embodiment 2 (man-made feeds II)
1, getting 4g brewer's yeast is dissolved in 30g distilled water, then 15g stem tea powder is added successively, 4g sucrose, 2.5g glucose, 0.1g Choline Chloride, 0.2g methyl p-hydroxybenzoate, 0.8g Webster salt stir, and then add 0.4g vitamin C, 0.04g vitamin E, 0.01g Cobastab mixed liquor, mix.
2, agar powder is dissolved in 50 hot water boiling and rises 2-3 minute, then be poured in mixture solution described in 1, the culture dish got ready is poured rapidly into after stirring, rocking culture dish makes feed bedding bottom culture dish even, after agar solidification with pocket knife be divided into strip drop into raise tea geometrid crisper inside upper strata gauze on feed.
The preparation method of the Cobastab mixed liquor described in embodiment 1,2 is, p-to 0.1g folic acid, 0.05g core yellow acid and 0.05g amido benzoic acid is put into small beaker, add 5-10ml distilled water to stir, add the potassium hydroxide solution that 1-2 drips 1N, be stirred to solution transparent, add 0.1g nicotinic acid again, 0.05g puridoxine hydrochloride, 0.1g biotin be made into Cobastab mixed liquor, put into refrigerator for subsequent use.
In order to better verify practical application effect of the present invention, illustrate that feed formula of the present invention and tealeaves are raised tea geometrid growth and development state and contrasted with example below.
Embodiment 3
For examination formula: the tea geometrid feed prepared by embodiment 2
Experimental control: tealeaves is raised
Test grouping: tea geometrid feed (A) prepared by embodiment 1, the tea geometrid feed (B) of embodiment 2 preparation, fresh tea leaf in its contrast (C) 3 process, each process repeats for 5 times, totally 15 culture dishes are raised, each culture dish inoculation looper 10.Within every 3 days, change and raise once.Table 1 compares for man-made feeds and natural feed tea geometrid development duration.As can be seen from Table 1, can grow normally under the tea geometrid diet condition that tea geometrid is prepared in an embodiment, generation time raises about long 5-7 days than natural feed.
Table 1 man-made feeds and natural feed tea geometrid development duration compare
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (2)
1. a preparation method for tea geometrid feed, is characterized in that, its step is as follows:
(1) beer yeast powder is dissolved in distilled water, then add stem tea powder successively, sucrose, glucose, Choline Chloride, methyl p-hydroxybenzoate, Webster salt stirs, and then add vitamin C, vitamin E, Cobastab mixed liquor, mixing and stirring;
(2) agar powder is dissolved in hot water boiling rises 2-3 minute, then be poured in the mixture solution described in (1), the culture dish got ready is poured rapidly into after stirring, rocking culture dish makes feed bedding bottom culture dish even, after agar solidification, be divided into strip with pocket knife;
In the method, each component calculates by weight, beer yeast powder 5 parts-10 parts, stem tea powder 20-30 part, sucrose 3-10 part, glucose 2.5 parts-5 parts, Choline Chloride 0.05-0.1 part, methyl p-hydroxybenzoate 0.2 part-0.5 part, Webster salt 0.5-2 part, vitamin C 0.1 part-1 part, vitamin E 0.03 part-0.08 part, Cobastab mixed liquor 0.006 part-0.01 part, agar powder 3-6 part, water 70-120 part;
Wherein, the preparation method of described Cobastab mixed liquor is, 0.1g folic acid, 0.05g core yellow acid and 0.05g Para-Aminobenzoic are put into small beaker, add 5-10ml distilled water to stir, add the potassium hydroxide solution that 1-2 drips 1N, be stirred to solution transparent, then add 0.1g nicotinic acid, 0.05g puridoxine hydrochloride, 0.1g biotin be made into Cobastab mixed liquor, puts into refrigerator for subsequent use.
2. the tea geometrid feed that obtains of preparation method according to claim 1.
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| CN201410174219.3A CN103947879B (en) | 2014-04-28 | 2014-04-28 | A kind of tea geometrid feed and preparation method thereof |
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| CN201410174219.3A CN103947879B (en) | 2014-04-28 | 2014-04-28 | A kind of tea geometrid feed and preparation method thereof |
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| CN103947879A CN103947879A (en) | 2014-07-30 |
| CN103947879B true CN103947879B (en) | 2015-10-28 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106819664A (en) * | 2017-03-30 | 2017-06-13 | 青岛海之星生物科技有限公司 | A kind of tea geometrid larvae artificial feeding feed and preparation method thereof |
| CN109170384A (en) * | 2018-11-15 | 2019-01-11 | 信阳农林学院 | A kind of tea plant pest ash tea geometrid man-made feeds and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1954669A (en) * | 2005-10-28 | 2007-05-02 | 中国林业科学研究院森林保护研究所 | Artificial feeding and generation method of Ectropis oblique hypulin W. and artificial feed for its larva |
| CN101044885A (en) * | 2007-04-29 | 2007-10-03 | 浙江省农业科学院 | Artificial feed for tea plant looper, its preparing method, and method for raising said loopers |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1954669A (en) * | 2005-10-28 | 2007-05-02 | 中国林业科学研究院森林保护研究所 | Artificial feeding and generation method of Ectropis oblique hypulin W. and artificial feed for its larva |
| CN101044885A (en) * | 2007-04-29 | 2007-10-03 | 浙江省农业科学院 | Artificial feed for tea plant looper, its preparing method, and method for raising said loopers |
Non-Patent Citations (3)
| Title |
|---|
| 人工饲料饲养茶尺蠖简报;柳德宝等;《昆虫知识》;19820531(第5期);第36-37页 * |
| 茶尺蠖人工饲料的研究初报;赵烨烽等;《中国茶叶》;19820630(第6期);第14-15页 * |
| 茶尺蠖幼虫在茶叶和人工饲料上的取食行为;李云寿等;《云南农业大学学报》;19900630;第5卷(第2期);第89-96页 * |
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