CN104430541A - Method for pest trapping with insect pheromone - Google Patents
Method for pest trapping with insect pheromone Download PDFInfo
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- CN104430541A CN104430541A CN201310417852.6A CN201310417852A CN104430541A CN 104430541 A CN104430541 A CN 104430541A CN 201310417852 A CN201310417852 A CN 201310417852A CN 104430541 A CN104430541 A CN 104430541A
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Abstract
The invention discloses a method for pest trapping with insect pheromone. The method includes: first step, preparation of a food extract: crushing food with a machine, weighing 10g of the food and soaking it in 100mL dichloromethane, putting the mixture in a 250mL stopper equipped conical flask (subjected to 200DEG C high temperature drying before use) to conduct soaking for 24h, performing filtering with filter paper or glass wool, then concentrating the solution to 10mL with nitrogen, and conducting preservation at a temperature of -20DEG C; second step, preparation of pheromone: drying the stopper equipped conical flask at 200DEG C high temperature, placing male pests subjected to male and female distinguishment into a 250mL stopper equipped conical flask, adding 100mL dichloromethane to conduct soaking at 25DEG C for 24h, then removing the pest bodies, carrying out filtering with filter paper or glass wool, and then concentrating the solution to 10mL with nitrogen, and conducting preservation at a temperature of -20DEG C; and third step, placing the insect pheromone and the food extract into a glass culture dish and performing mixing.
Description
Technical field
The invention belongs to insect method, relate to the extracting method of a kind of insect pheromone trapping insect.
Background technology
Interactional chemical substance between pheromones (pheromone) individuality of the same race, can affect behavior each other, habit and even grow and physiological activity.Pheromones is manufactured by body inner gland body, and directly discharge is dispersed into external, and pheromones relies on the conductive medium such as air, water to pass to other individualities.Pheromones is had from lower animal to higher mammal.Due to the outer environment transmission of pheromones, therefore also known as pheromone.Between biological xenogenesis, interactional chemical substance is called allomone or allomone.Allomone between insect has allomone, kairomone, semaphorins etc.Pheromones major sexual pheromones, aggregation pheromone, warning information element, trail, marking pheromone etc.
Pheromones is the general designation of the compound playing chemical communication effect between organism, is the chemical molecular language that insect exchanges.Comprise: kairomone, allomone, collaborative element, assembly pheromone, trail pheromone, warning information element, epideictic pheromone, sex pheromone.At occurring in nature, insect female adult pest is after sexual maturity, a kind of compound being sex pheromone can be discharged, it is released into after in air with Diffusion of gas stream, stimulate the chemical sensation organ in male worm feeler, cause male sexual drive and lure male worm to the flight of source of release orientation, and with the mating of release female adult pest to produce offspring.Therefore, insect sex attractant product is bionical high-tech product, by the sex pheromone luring core to discharge Prof. Du Yucang.
Insect pheromone be insect be used for representing assembles, look for food, mating, the various information such as warning compound, be the chemical molecular language of insect interchange.Wherein insect sex pheromone is the compound that regulation and control insect male and female attract behavior, not only sensitivity but also single-minded, and operating distance is far away, and temptation is strong.Gyplure is the insect sex pheromone of simulating nature circle, is discharged into by release the bionical high-tech product that different in nature insect is trapped and killed in field.This technology insect-killing trapping does not contact plant and agricultural product, does not have the sorrow of residue of pesticide, is one of prefered method of Modern agricultural ecological pest control.
Insect sex pheromone is as the chemical communication instrument of reproductive phase between different in nature insect of the same race, the high complexity of its chemical constitution and particularity are the important guarantees of reproduction isolation between insect kind, therefore, sex pheromone between different insects can not substitute mutually, also can not cause and obscure, this regionality insect is investigated, there is larger practical value in plant quarantine and mass trapping etc.
Patent 201210218955.5 discloses a kind of Against Conopomorpha sinensis Bradley sex pheromone of Prof. Du Yucang and lures core, it is characterized in that adopting trans-4, 6-cis-10-16 carbon triolefin acetic acid esters, trans-4-cis-6, 10-16 carbon triolefin acetic acid esters, trans-4, 6-cis-10-16 carbon three enol, trans-4-cis-6, 10-16 carbon three enol, positive hexadecanol mixes according to parts by weight ratio 0.1 ~ 3:12 ~ 25:1 ~ 5:1 ~ 15:12 ~ 50, dissolve with acetone equal solvent, getting dosage is 50 ~ 3000 micrograms, add by natural rubber, synthetic rubber, polyethylene, polyvinyl chloride, paper material, in the slow-released carrier that resin or viscose material etc. are made.Good effect of the present invention is: be applied to the control of Against Conopomorpha sinensis Bradley damage by disease and insect and observe and predict, greatly improve sex pheromone to the attractive activity of Against Conopomorpha sinensis Bradley, trapping success rate is high, be conducive to industry synergy, orchard worker increases income, and agriculture ghost sound food security can not be there is, free from environmental pollution, do not destroy the ecological balance, be conducive to the sustainable development of promotion industry.
Summary of the invention
1, object of the present invention.
The present invention, in order to effectively eliminate the insect in deposit food, proposes a kind of method of pheromones trapping pests.
2, the technical solution adopted in the present invention.
Insect pheromone trapping insect method:
The preparation of a, food extract
Food machine is pulverized, takes 10 g, 100 mL carrene and soak, be put in (before use through 200 DEG C of hyperthermia dryings) in the tool plug conical flask of 250 mL, soak 24 h, filter with filter paper or mineral wool, then with nitrogen, solution is concentrated into 10 mL, under being kept at the temperature of-20 DEG C;
The preparation of b, pheromones
Tool plug conical flask is through 200 DEG C of hyperthermia dryings, by the insect male worm after male and female are differentiated, put into the tool plug conical flask of 250 mL, add 100 mL carrene to soak, soak 24 h under being placed in the condition of 25 DEG C, then remove polypide, filter with filter paper or mineral wool, with nitrogen, solution is concentrated into 10 mL again, storage temperature is-20 DEG C;
C, trapper
With glass culture dish, put into insect pheromone and mix with food extract.
Described insect comprises corn weevil, rice weevil, lesser grain borer, saw-toothed grain beetle, red plan paddy, rusty grain beetle.
Pheromone extract dosage adopts 20-60 μ L.Temperature is 28 ± 1 DEG C, and relative moisture is 75 ± 5%.
Preferably, pheromone extract dosage adopts 20 μ L, 30 μ L, 40 μ L, 50 μ L, a 60 μ L5 gradient.Best information extract dosage is 40 μ L.
3, beneficial effect of the present invention.
The present invention deposits in case at multiple grain storage pest, and food extract mixes with insect pheromone extract except having significant synergistic function to the trapping of insect of the same race, also has good trap effect to the insect of other kind.
Embodiment
Embodiment
Insect pheromone trapping insect method:
The preparation of a, food extract
Food machine is pulverized, takes 10 g, 100 mL carrene and soak, be put in (before use through 200 DEG C of hyperthermia dryings) in the tool plug conical flask of 250 mL, soak 24 h, filter with filter paper or mineral wool, then with nitrogen, solution is concentrated into 10 mL, under being kept at the temperature of-20 DEG C;
The preparation of b, pheromones
Tool plug conical flask is through 200 DEG C of hyperthermia dryings, by each 300 of kind of the insect male worm of 6 after male and female are differentiated, put into the tool plug conical flask of 250 mL respectively, add 100 mL carrene to soak, soak 24 h under being placed in the condition of 25 DEG C, then remove polypide, filter with filter paper or mineral wool, with nitrogen, solution is concentrated into 10 mL again, storage temperature is-20 DEG C;
C, trapper
With glass culture dish, put into insect pheromone and mix with food extract.
Described insect comprises corn weevil, rice weevil, lesser grain borer, saw-toothed grain beetle, red plan paddy, rusty grain beetle.
Pheromone extract dosage adopts 20-60 μ L.Temperature is 28 ± 1 DEG C, and relative moisture is 75 ± 5%.
Preferably, pheromone extract dosage adopts 20 μ L, 30 μ L, 40 μ L, 50 μ L, a 60 μ L5 gradient.Best information extract dosage is 40 μ L.
It is better to the trap effect of insect of the same race when corn weevil, rice weevil, saw-toothed grain beetle, rusty grain beetle pheromone extract dosage are 40 μ L, on average trap 6.2,6.3,6.5,6.4 respectively, significant difference (P<0.05) compared with traping insect effect of the same race with other dosage, the trap effect of red flour beetle and lesser grain borer increases along with the increase of pheromone extract dosage.The lesser grain borer number of traping when lesser grain borer pheromone extract dosage is 50 μ L is maximum, on average traps to 4.5, significant difference (p<0.05) compared with other dosage trap effect.The red flour beetle effect that red flour beetle pheromone extract dosage is traped when 40 μ L and 50 μ L is better, wherein on average traps 7.0 when 50 μ L.
Food extracts mixes with the pheromone extract of a kind of insect and to mix with the pheromone extract of a kind of insect the trap effect result of study of various pests in shown in table 1 the trap effect food extracts of various pests.
As can be seen from table 1, food extracts is 13.0 ± 0.6 to the trapping of corn weevil; It is 10.3 ± 0.4 to the trapping of rice weevil; It is 4.2 ± 0.5 to the trapping of lesser grain borer; It is 6.8 ± 0.5 to the trapping of rusty grain beetle; The trapping of red flour beetle is 8.8 ± 0.3, is 6 ± 0.4 to the trapping of saw-toothed grain beetle.When food extracts mixes except having significant synergistic function to the trapping of lesser grain borer with lesser grain borer pheromone extract, also there is certain trap effect to other grain storage pest.Deposit in case in various pests, food extracts mixes with insect pheromone extract except having significant synergistic function to the trapping of insect of the same race, also has good trap effect to the insect of other kind.
The threshold of reaction this test certain along with the increase of dosage shows to attractant effect of insect pheromone extract adopts dichloromethane solvent infusion method 6 kinds of grain storage pests to be carried out to the extraction of pheromone extract, using extract as main experimental material, respectively dosage be 20 μ L, 30 μ L, 40 μ L, 50 μ L, 60 μ L time carry out biological activity determination.Insect pheromone extract all has obvious trapping effect to insect of the same race, namely has the existence of pheromones in insect pheromone extract.In independent trapping experiments process, corn weevil, rice weevil, become rusty red limit ostomatid and the trap effect of saw-toothed grain beetle when pheromone extract dosage is 40 μ L best, significant difference (p<0.05) compared with the trapping result under other dosage conditions, 2 is 50 μ L and 60 μ L at dosage, and trap effect does not have 40 μ L fashions, when other 2 kinds of grain storage pests are then presented at 50 μ L, trap effect is best, its reason may be that the reaction of 6 kinds of examination worms to respective pheromone extract has a threshold value, too high or the too low trap effect demonstrated of pheromone concentration, the pheromones of high concentration may reduce caused by its luring property.The luring property of lesser grain borer pheromones impact to lesser grain borer of variable concentrations.Compare with n-hexane, high concentration pheromones reduces it and lures worm effect.This shows, should consider the problem of a dosage and concentration when practical application, the amount of pheromones is not The more the better.Food extracts mixes with the pheromone extract of insect to be studied food extracts to various pests trap effect and mixes the attractant effect that can not only strengthen insect of the same race with the pheromone extract of a kind of insect, and also has good attracting action to other insect.Lesser grain borer pheromones can not only attract lesser grain borer, and can attract corn weevil; Indian meal moth pheromones can not only attract Indian meal moth, and can attract lesser grain borer.Apply 6 kinds of grain storage pest pheromone extracts in this process of the test to mix with food extracts respectively and trap multiple grain storage pest, find when various pests coexists, food extracts mixes with insect pheromone extract the trap effect trapping of insect of the same race having been exceeded to the pheromone extract being used alone food extracts or insect of the same race, and also has more significant humidification for the trapping of other insect.As food extracts mixes except traping except humidification to rice weevil with rice weevil pheromone extract, also there is good trapping effect to corn weevil; Rusty grain beetle pheromone extract mixes with food extracts except having significant humidification to insect of the same race, also has good attractant effect to saw-toothed grain beetle, red flour beetle; But it is but not too obvious to the attractant effect of rusty grain beetle when saw-toothed grain beetle pheromone extract mixes with food extracts.Particularly when lesser grain borer pheromone extract mixes with food extracts except having significant humidification to lesser grain borer, also having certain trap effect but being not all very desirable other insect.
In sum, when food extracts mixes with insect pheromone extract, except the trapping effect of insect of the same race is significantly strengthened, also there is good attractant effect to the trapping of other insect.Insect pheromones lures worm property to be better than single pheromones or food attractant with food attractant or food odors volatile matter in conjunction with it.Food attractant obviously can strengthen the trap effect of pheromones to insect.The mixture of insect pheromone and food extracts is placed in this experiment in same trapper, for the sensitivity improving trapper, the monitoring effect strengthening insect has very important meaning, provides certain theoretical foundation for developing mixed type attractant that is efficient, wide spectrum simultaneously.
Above-described embodiment does not limit the present invention in any way, and the technical scheme that the mode that every employing is equal to replacement or equivalent transformation obtains all drops in protection scope of the present invention.
Claims (6)
1. an insect pheromone trapping insect method, is characterized in that:
The preparation of a, food extract
Food machine is pulverized, takes 10 g, 100 mL carrene and soak, be put in (before use through 200 DEG C of hyperthermia dryings) in the tool plug conical flask of 250 mL, soak 24 h, filter with filter paper or mineral wool, then with nitrogen, solution is concentrated into 10 mL, under being kept at the temperature of-20 DEG C;
The preparation of b, pheromones
Tool plug conical flask is through 200 DEG C of hyperthermia dryings, by the insect male worm after male and female are differentiated, put into the tool plug conical flask of 250 mL, add 100 mL carrene to soak, soak 24 h under being placed in the condition of 25 DEG C, then remove polypide, filter with filter paper or mineral wool, with nitrogen, solution is concentrated into 10 mL again, storage temperature is-20 DEG C;
C, trapper
With glass culture dish, put into insect pheromone and mix with food extract.
2. insect pheromone trapping insect method according to claim 1, is characterized in that: described insect comprises corn weevil, rice weevil, lesser grain borer, saw-toothed grain beetle, red plan paddy, rusty grain beetle.
3. insect pheromone trapping insect method according to claim 1 and 2, is characterized in that: pheromone extract dosage adopts 20-60 μ L.
4. insect pheromone trapping insect method according to claim 1, it is characterized in that: temperature is 28 ± 1 DEG C, relative moisture is 75 ± 5%.
5. insect pheromone trapping insect method according to claim 3, is characterized in that: pheromone extract dosage adopts 20 μ L, 30 μ L, 40 μ L, 50 μ L, a 60 μ L5 gradient.
6. insect pheromone trapping insect method according to claim 5, is characterized in that: pheromone extract dosage adopts 40 μ L.
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| CN201310417852.6A CN104430541A (en) | 2013-09-15 | 2013-09-15 | Method for pest trapping with insect pheromone |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106561359A (en) * | 2016-11-19 | 2017-04-19 | 福建省农业科学院植物保护研究所 | Method for combined prevention and treatment of aphis pests by using pheromone complex and ladybugs |
| CN107432279A (en) * | 2016-05-27 | 2017-12-05 | 河北省农林科学院植物保护研究所 | Araecerus fasciculatus aggregation pheromone composition and its application |
| CN115053896A (en) * | 2021-06-02 | 2022-09-16 | 吉林农业大学 | Preparation method of insect trapping agent |
-
2013
- 2013-09-15 CN CN201310417852.6A patent/CN104430541A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107432279A (en) * | 2016-05-27 | 2017-12-05 | 河北省农林科学院植物保护研究所 | Araecerus fasciculatus aggregation pheromone composition and its application |
| CN107432279B (en) * | 2016-05-27 | 2019-11-01 | 河北省农林科学院植物保护研究所 | Araecerus fasciculatus aggregation pheromone composition and its application |
| CN106561359A (en) * | 2016-11-19 | 2017-04-19 | 福建省农业科学院植物保护研究所 | Method for combined prevention and treatment of aphis pests by using pheromone complex and ladybugs |
| CN106561359B (en) * | 2016-11-19 | 2019-11-19 | 福建省农业科学院植物保护研究所 | A method of combining prevention and treatment phytophthira pest with ladybug using pheromones compound |
| CN115053896A (en) * | 2021-06-02 | 2022-09-16 | 吉林农业大学 | Preparation method of insect trapping agent |
| CN115053896B (en) * | 2021-06-02 | 2023-05-16 | 吉林农业大学 | Method for preparing insect trapping agent |
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Application publication date: 20150325 |