CN101785486A - Non-toxic, colorless and odorless biological water aqua for trapping fruit fly and preparation method thereof - Google Patents
Non-toxic, colorless and odorless biological water aqua for trapping fruit fly and preparation method thereof Download PDFInfo
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- CN101785486A CN101785486A CN201010131326A CN201010131326A CN101785486A CN 101785486 A CN101785486 A CN 101785486A CN 201010131326 A CN201010131326 A CN 201010131326A CN 201010131326 A CN201010131326 A CN 201010131326A CN 101785486 A CN101785486 A CN 101785486A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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Abstract
The invention discloses a non-toxic, colorless and odorless biological water aqua for trapping fruit flies and a preparation method thereof. The biological water aqua is prepared through mixing sex pheromones and aggregation pheromones according to a proportion, adding inhibitory enzyme and carrying out development through a complex process, and belongs to the field of biological pharmacy. The invention solves the problem of the effective period, and the longest effective period can reach 60 days, thereby greatly reducing the use cost of peasants. In addition, the biological water aqua can be used very conveniently, and can be directly sprayed onto leaves of fruit trees. Since no toxicity or residue exists, the biological water aqua can no affect the quality of fruits. Due to the use of the inhibitory enzyme, the targeting effect is unique, and the biological aqua has no toxic side effects on insects and organisms except for the targets.
Description
Technical field
The present invention relates to a kind of aqua and preparation method of biological trapping fruit fly of non-toxic, colorless and odorless, belong to field of biological pharmacy.
Background technology
Fruit fly is China two classes quarantine property insect, is commonly called as golden fly, pin honeybee, orient fruit fly.This fly profile and fly are approximate, but the bodily form is littler than fly, all golden yellow colour band black spots lines, and reproduction speed is fast, and fulminant is strong, and is dangerous big.Adult lays eggs in pericarp, and larva eats into food in pulp, causes rotting and shedding of fruit such as sugar-apple, guava, banana, mango, pawpaw, carambola, citrus, sweet shaddock, oily a kind of apple, has a strong impact on quality and yield, is described as " the No.1 killer " of fruit.
Fruit fly is distributed in Hawaii, the island, Pacific Rim of Australia, New Zealand, the U.S., the Egypt in my each provinces and regions of southeastern coast and Burma, Cambodia, Laos, Vietnam, TaiWan, China, Japan, Indonesia, Malaysia, Thailand, Brunei, India, Bangladesh, Nepal, Sri Lanka, Pakistan, Iran, Afghanistan, Azerbaijan, Africa, Kenya, Tanzania, Mauritius, Papua New Guinea, New Ireland or the like various countries, global warming has aggravated spreading of this clock small insect.Classified as important quarantine insect by countries in the world for a long time always.Fruit fly mainly contains following two aspects to the harm of melon and fruit industry: directly can cause fruit rot, shedding after the production fruit fly infringement melon and fruit of harm melon and fruit industry on the one hand, have a strong impact on the output and the quality of melon and fruit.Can make output descend more than 40% when serious.On the other hand, have a strong impact on the market access: because the serious harm of fruit fly thereby be that countries in the world are as the surging monoid of adventive invasion and the strick precaution emphasis of port quarantine all the time.Even if even fruit itself do not encroached on by fruit fly but since the place of production close on the fruit fly disaster area and promptly can be listed in the limiting inlet product and strictly take precautions against and then lose the chance that enters the international market by importer.The strick precaution of fruit fly has become the particularly important topic of fruit industry developed country of various countries, the whole world thus.
Fruit fly more generally takes place at the fruit growing area, and the prophylactico-therapeutic measures that present people find has: 1 chemical control The Application of Technology comprises direct sprinkling agricultural, with the poison fruit trapping of soaking medicine etc., this Prevention Technique DeGrain, the residue of pesticide phase is long and big, easily causes the person poultry poisoning.2 physical containments comprise technology such as covering fly net, bagging insect protected fruit retention, and this technology construction is complicated, the cost height, and effect is general.The method that 3 usefulness are emitted no pregnant male worm prevents the breeding of fruit fly, and this technical know-how is feasible, and practical operation is difficulty very, and is invalid substantially.4 chemical pesticide direct additive pheromone attractant are come adult extremely, and this technology is generally to adopt present stage, and effect is obvious, but can only trap and kill male worm, and the term of validity short (3-5 days), the complexity of layouting, and chemical pesticide medicament residue height directly has influence on the edible of melon and fruit.Along with the raising of environmental consciousness, non-harmful pure green attractant is being called in market.
Summary of the invention
Therefore, people are to control efficiency is better, the term of validity is longer, the pure green non-pollution attractant expectation demand of the two property trappings of energy is increasing, especially advocate under the environment of pollution-free food, green planting in country, the application prospect of inhibitory enzyme and insect sex pheromone will be more wide.Up to now, also do not find the report of any relevant pharmaceutical composition of the present invention.The inventor is through research repeatedly, and the checking repeatedly by the animal clinical testing, has found the medicine that better control fruit fly is arranged finally, thereby has finished the present invention.
Purpose of the present invention just provides a kind of a kind of pure green non-pollution attractant composition of more effectively preventing and treating fruit fly.
Another object of the present invention has provided this attractant preparation of compositions method.
Attractant is to adopt sex pheromone and aggregation pheromone, according to than row interworking, adds inhibitory enzyme and develops through complicated technology.At first solved the problem of the term of validity, the most long-acting fruit can reach 60 days, greatly reduces friend's peasant use cost, and is very easy to use in addition, gets final product after being sprayed directly on the fruit tree leaf, and nontoxic, noresidue does not influence fruit quality.Because the employing inhibitory enzyme, targeting is single, to the insect beyond the target and biological without any side effects.
1) inhibitory enzyme reparation technology flow process is: source of infection separation and purification----enlarged culture-----concentrate deactivation,--------the insect biological fluid extracting------essence leaches bacterium, and------packing is to be checked----, and put in storage in powder process in freeze-drying in deactivation in centrifugation by the inhibitory enzyme finished product in the enzyme detection in the insect group infection to add adjuvant.Thereby inhibitory enzyme can suppress the activity of the protective enzyme in the fruit fly body causes enzymatic breaking
2) interpolation of pheromones: pheromones is to play the general designation of the compound of chemical communication effect between the organism, is the chemical molecular language that insect exchanges.At occurring in nature, the insect female adult worm is after sexual maturity, can discharge a kind of compound that is sex pheromone, it spreads with air-flow after being released in the air, stimulate the chemical sensation organ in the male worm feeler, cause the male sexual drive and lure male worm to the directed flight of source of release, and with discharge the female adult worm mating to produce offspring.Therefore, the insect sex attractant product is bionical high-tech product, discharges the sex pheromone of synthetic by luring core.Gyplure is the insect sex pheromone of simulating nature circle, is discharged into the bionical high-tech product that different in nature insect is trapped and killed in the field by release.This technology insect-killing trapping does not contact plant and agricultural product, does not have the sorrow of residue of pesticide, is one of prefered method of modern agriculture bionomic control insect.
Sex pheromone is as the chemical communication instrument of reproductive phase between the different in nature insect of the same race, the high complexity of its chemical constitution and particularity are the important assurances that reproduction is isolated between the insect kind, therefore, sex pheromone between the different insects can not substitute mutually, can not cause and obscure that aspects such as this regionality investigation insect, plant quarantine and trapping control have bigger practical value.The present invention proportionally adds sex pheromone and aggregation pheromone in preparation, be only to lure at the single melon and fruit trypetid of target, and the biology and the natural enemy of other non-targets had no effect.
3) Chinese herbal medicine extract:
The leaching that utilizes cavitation effect of ultrasonic waves to quicken effective ingredients in plant is extracted, hyperacoustic in addition second order effect, can quicken also that desire extracts that the diffusion of composition discharges and fully and solvent, be beneficial to extraction as mechanical oscillation, emulsification, diffusion, broken, chemical effect etc.Test method, carried out optimizing selection with the extraction conditions of supercritical liquid extraction technique to nimbin, mother chrysanthemum, optimum extraction condition is pressure 34.smPa as a result, 60 ℃ of temperature, modifier ethanol 0.3ml, static extracting time 10min, dynamic extraction amount 10ml, with water as absorption.Nimbin, mother chrysanthemum are as safe as a house to non-target organisms such as mammals.
Medicine of the present invention is to people and animal nonhazardous, and is free from environmental pollution, is a kind of green product of compliance with environmental protection requirements.This lure has pure and mild dense aromatic odor, and fruit fly is had the stronger kill activity of luring, and the buying of this medicament stock is convenient, low production cost, and technology is simple, and advantage therefore low-cost and the environment-protecting asepsis evil has the very strong market competitiveness.
Embodiment
Below further set forth the beneficial effect of medicine of the present invention by testing example, these are tested examples and have comprised that the safety testing (acute toxicity experience and subchronic toxicity test) of medicine of the present invention (to call the killer in the following text No. 1) and clinical observation test.
[test example 1] medicine of the present invention is to the acute toxicity experience of small white mouse
1 test material
1.1 for the reagent thing
Killer No. 1, the inferior medicine company of Hunan prestige Science and Technology Ltd. produces lot number: 20090610
1.2 for the examination animal
50 of healthy mices, male and female half and half, body weight are 18-22g, available from laboratory animal portion of Central South University.
2 examinations, 5 proved recipe methods
50 small white mouses are divided into 5 groups at random, and 10 every group, male and female half and half are pressed the dose of 1g/kg body weight, 2g/kg body weight, 5g/kg body weight, 8g/kg body weight, five dosage groups of 12g/kg body weight respectively No. 1 one natural gift of killer are irritated the stomach mouse 5 times.After the administration, observe and write down the toxic reaction that animal is occurred in the 7d, add up the dead animal number and, then carry out just inspection of histology, use improvement karber's method calculating LD if any pathological tissues to the timely postmortem of dead animal
50And 95% average fiducial limit.
3 results and analysis
The The acute toxicity tests that table 1 killer is No. 1
| Group | Dosage g/kg body weight | Number of animals/only | Death toll/only | Lethality/% | Survival rate/% |
| ??1 | ??1 | ??10 | ??0 | ??0 | ??100 |
| ??2 | ??2 | ??10 | ??0 | ??0 | ??100 |
| ??3 | ??5 | ??10 | ??0 | ??0 | ??100 |
| ??4 | ??8 | ??10 | ??1 | ??10 | ??90 |
| ??5 | ??12 | ??10 | ??2 | ??20 | ??80 |
The acute toxicity tests sees Table 1.The result shows: behind the mice lavage medicine, first group, second group and the 3rd group all survives, and clinical manifestation is normal; The 4th group dead in 24h 1; The 5th group dead in 12h 2.Poisoning small white mouse cardinal symptom is depressed, the expiratory dyspnea of spirit, based on abdominal respiration, dead small white mouse is cutd open inspection, and finding has gasteremphraxis and bulge phenomenon, and remaining tissue there is no unusually.
Calculate No. 1 median lethal dose (LD for oral administration of killer by the improvement karber's method
50) be the 72g/kg body weight, the 95% average credible 68.4-80g/kg body weight that is limited to is judged the basic toxic formulation that is by the toxicity criterion.
4 conclusions
The killer gives the median lethal dose (LD of mice lavage for No. 1
50) be the 72g/kg body weight, be basic toxic formulation by safety of medicine toxicological evaluation standard, (1-12g/kg body weight) is safe in the medical treatment scope.
[test example 2] medicine of the present invention is to the subchronic toxicity test of big white mouse
1 test material
1.1 for the reagent thing
Killer No. 1, the inferior medicine company of Hunan prestige Science and Technology Ltd. produces lot number: 20090610
1.2 for the examination animal
50 of healthy white rats, male and female half and half, body weight are 120-128g, available from laboratory animal portion of Central South University.
2 test methods
Irritate stomach killer No. 1 0.5g/kgd, 2g/kgd (be equivalent to clinical administration dosage 5 times, 20 times) respectively to big white mouse, continuous four months, observe and the record toxic reaction that animal occurred, to physical signs inspections such as the blood routine examination of big white mouse, liver function test, Electrocardioscopy, and anatomic observation is respectively organized the pathological change of internal organs during off-test.
3 results and analysis
3.1 the general activity after the two dosage group big white mouse administrations, the state of mind, hair, ight soil etc. there is no notable difference.
3.2 peripheral hemogram before and after the administration comprises that red blood cell, leucocyte, erythrocyte, packed cell volume all fluctuate in normal range (NR).
3.3 three livers (SGPT, SGOT), kidney (urea nitrogen, creatinine) functional parameter be there is no obvious influence.
3.4 during off-test, the content of total protein, albumin, alkaline phosphatase is compared no significant difference with control group in the animal blood serum.
3.5 Electrocardioscopy is all no abnormal.
3.6 each main organs visual inspection is morphological changes of various tissue components such as obviously enlargement, hyperemia, necrosis not.
3.7 the weight coefficient of the heart, liver, spleen, lung, kidney, thymus gland, suprarenal gland, uterus, testis is compared with control group, no significant difference.
3.8 histopathologic examination there is no unusual.
3.9 this medicine of above presentation of results is safer, in institute's amount of reagent scope.
4 conclusions
The subchronic toxicity test result of study shows: irritate stomach killer No. 1 0.5g/kgd, 2g/kgd (be equivalent to clinical administration dosage 5 times, 20 times) respectively to big white mouse, continuous four months, general activity after the administration of two dosage group big white mouse, physical signs inspections such as blood routine examination, liver function test, Electrocardioscopy, respectively organize the pathological change of internal organs to there is no unusually, judge the basic toxic formulation that is by the toxicity criterion, (0.1-2g/kg body weight) is safe in the medical treatment scope.
The clinical efficacy test of [test example 3] medical treatment fruit fly of the present invention
Test unit: Guangdong Province's plant protection unit
It is comparatively serious that China's melon and fruit class was subjected to fruit fly harm in recent years, tentatively controlled by the technical measures of taking to be correlated with, and wherein male trapping is a main prophylactico-therapeutic measures.For seeking better attractant, the inferior male and female double play attractant " steady glutinous " with Guangdong plant protection unit co-production of Hunan on the 11st prestige September in 2009 carries out field trial.Now result of the test is reported as follows.
1 materials and methods
1.1 examination material
" killer No. 1 " male and female double play fruit fly attractant, Wei Ya pharmaceutcal corporation, Ltd product; Lure fly ether (sex attractant, trapping male worm), the Guangdong Province Insects Research Institute product.Test is carried out carambola garden area 4028m in 4 years living carambola of Denghai District Lian Xia town, Shantou City, Guangdong Province Chinese scholartree pool directorial area garden
2
1.2 test is provided with
" killer No. 1 " with lure fly ether effect comparative trial.If two every 667m2 of processing: A. are provided with 15 points of " killer No. 1 " trapping, " killer No. 1 " 6～7ml is sprayed at leaf and fruit surface; B. every 667m2 is provided with and lures 4 in fly ether trapping bottle, and every bottle adds and lures fly ether 2ml and dichlorvos 2ml.To trap and kill bottle and be suspended on below the tree crown overhead 1.5m place, be separated by 10 meters for every bottle.Duration of test weather is fine, 20～28 ℃ of temperature.
2 results and analysis
Result of the test is found out, " killer No. 1 " attractant trapping fruit fly effect lures fly ether good than gyplure, each puts 107.25 of average trapping fruit flies (take in borer population dead on the film and be as the criterion to fall the plastics of transferring placement at leaf) 5 days accumulative total, be to lure 8.25 times of 13 of fly ether trappings, than 1: 1, the quantity of the trapping of " killer No. 1 " hero fly is to lure 4 times of fly ether by female male worm property.
Claims (3)
1. aqua and the preparation method of the biological trapping and killing flies of a non-toxic, colorless and odorless and melon and fruit trypetid, it is characterized in that it mainly is to be made by the raw material of following weight portion: contain 80 gram white sugar in 100 grams, 1 gram sex pheromone (suitable-the 9-tricosene), 1 gram gathering information, 10 gram inhibitory enzymes, 8 gram extracts (nimbin, each 2 gram of mother chrysanthemum).
2. according to the medicine of claim 1, wherein the consumption of each bulk drug is: white sugar 80 grams, sex pheromone (suitable-the 9-tricosene) 1 gram, gathering information 1 gram, inhibitory enzyme 10 grams, nimbin extract 2 grams, mother chrysanthemum 2 grams.
3. according to the preparation method of claim 1 or 2 described medicines, it comprises the following steps: 1)----enlarged culture-----concentrate deactivation that inhibitory enzyme reparation technology flow process is: source of infection separation and purification,------------the insect biological fluid extracting--------essence leaches bacterium, and--------packing is to be checked----, and put in storage, causes enzymatic breaking thereby inhibitory enzyme can suppress the activity of the protective enzyme in the fruit fly body in freeze-drying-powder process in deactivation in centrifugation by the inhibitory enzyme finished product in the enzyme detection in the insect group infection to add adjuvant;
2) interpolation of pheromones: pheromones is to play the general designation of the compound of chemical communication effect between the organism, it is the chemical molecular language that insect exchanges, at occurring in nature, the insect female adult worm is after sexual maturity, can discharge a kind of compound that is sex pheromone, it spreads with air-flow after being released in the air, stimulate the chemical sensation organ in the male worm feeler, cause the male sexual drive and lure male worm to the directed flight of source of release, and with discharge the female adult worm mating to produce offspring, therefore, the insect sex attractant product is bionical high-tech product, by luring core to discharge the sex pheromone of synthetic, gyplure is the insect sex pheromone of simulating nature circle, is discharged into the bionical high-tech product that different in nature insect is trapped and killed in the field by release, and this technology insect-killing trapping does not contact plant and agricultural product, the sorrow that does not have residue of pesticide is one of prefered method of modern agriculture bionomic control insect;
Sex pheromone is as the chemical communication instrument of reproductive phase between the different in nature insect of the same race, the high complexity of its chemical constitution and particularity are the important assurances that reproduction is isolated between the insect kind, therefore, sex pheromone between the different insects can not substitute mutually, can not cause and obscure that aspects such as this regionality investigation insect, plant quarantine and trapping control have bigger practical value;
3) Chinese herbal medicine extract:
The leaching that utilizes cavitation effect of ultrasonic waves to quicken effective ingredients in plant is extracted, and hyperacoustic in addition second order effect is as mechanical oscillation, emulsification, diffusion.Smash, chemical effect etc. also can be quickened diffusion release and abundant and the solvent that desire is extracted composition, is beneficial to extraction.Test method, with supercritical liquid extraction technique nimbin, mother chrysanthemum extraction conditions have been carried out optimizing and selected, optimum extraction condition is pressure 34.smPa as a result, 60 ℃ of temperature, modifier ethanol 0.3ml, static extracting time 10min, dynamic extraction amount 10ml, with water as absorption.Nimbin, mother chrysanthemum are as safe as a house to non-target organisms such as mammals.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155917A (en) * | 2013-03-26 | 2013-06-19 | 德清科中杰生物科技有限公司 | Housefly attractant |
| CN104304337A (en) * | 2014-09-05 | 2015-01-28 | 沧州科惠物理防虫研究所 | Plant source biotype fruit fly bisexual trapping and killing agent |
| CN105360085A (en) * | 2015-11-20 | 2016-03-02 | 泰山医学院 | Fly trapping and killing device and trapping and killing method thereof |
| CN105594747A (en) * | 2014-10-15 | 2016-05-25 | 浙江新安化工集团股份有限公司 | Precisely-oriented and efficient pest prevention and control insect disinfestation composition and use method thereof |
| WO2017207408A1 (en) * | 2016-05-30 | 2017-12-07 | Globachem Nv | Use of 9-tricosene to control drosophila suzukii |
-
2010
- 2010-03-24 CN CN201010131326A patent/CN101785486A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155917A (en) * | 2013-03-26 | 2013-06-19 | 德清科中杰生物科技有限公司 | Housefly attractant |
| CN103155917B (en) * | 2013-03-26 | 2014-08-20 | 德清科中杰生物科技有限公司 | Housefly attractant |
| CN104304337A (en) * | 2014-09-05 | 2015-01-28 | 沧州科惠物理防虫研究所 | Plant source biotype fruit fly bisexual trapping and killing agent |
| CN104304337B (en) * | 2014-09-05 | 2017-10-10 | 沧州佳和生物科技有限公司 | Plant source bion trypetid both sexes attractant |
| CN105594747A (en) * | 2014-10-15 | 2016-05-25 | 浙江新安化工集团股份有限公司 | Precisely-oriented and efficient pest prevention and control insect disinfestation composition and use method thereof |
| CN105360085A (en) * | 2015-11-20 | 2016-03-02 | 泰山医学院 | Fly trapping and killing device and trapping and killing method thereof |
| WO2017207408A1 (en) * | 2016-05-30 | 2017-12-07 | Globachem Nv | Use of 9-tricosene to control drosophila suzukii |
| BE1024239B1 (en) * | 2016-05-30 | 2018-01-09 | Globachem Nv | USE OF 9-TRICOSEEN TO COMBAT DROSOPHILA SUZUKII (SUZUKI'S FRUIT FLY) |
| CN109640654A (en) * | 2016-05-30 | 2019-04-16 | 环球化学股份有限公司 | 9- tricosene is used to control the purposes of spotted wing drosophila |
| EP3714693A1 (en) * | 2016-05-30 | 2020-09-30 | Globachem NV | Composition to control drosophila suzukii and method thereof |
| AU2017272383B2 (en) * | 2016-05-30 | 2020-12-17 | Globachem Nv | Use of 9-tricosene to control drosophila suzukii |
| CN109640654B (en) * | 2016-05-30 | 2021-04-13 | 环球化学股份有限公司 | Use of 9-eicosatriene for controlling Onyctada punctata |
| US10986835B2 (en) | 2016-05-30 | 2021-04-27 | Globachem Nv | Use of 9-tricosene to control Drosophila suzukii |
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Application publication date: 20100728 |