CN106561359A - Method for combined prevention and treatment of aphis pests by using pheromone complex and ladybugs - Google Patents

Method for combined prevention and treatment of aphis pests by using pheromone complex and ladybugs Download PDF

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CN106561359A
CN106561359A CN201611017469.1A CN201611017469A CN106561359A CN 106561359 A CN106561359 A CN 106561359A CN 201611017469 A CN201611017469 A CN 201611017469A CN 106561359 A CN106561359 A CN 106561359A
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ladybug
aphid
pheromone
complex
slow
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CN106561359B (en
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魏辉
田厚军
陈艺欣
陈勇
林硕
张艳璇
赵建伟
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Institute of Plant Protection of FAAS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00—Stationary means for catching or killing insects
    • A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Toxicology (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a method for combined prevention and treatment of aphis pests by using pheromone complex and ladybugs. An aphid alarm pheromone and a plant greenery odor are used as basic components and are prepared into a slow-release carrier by virtue of different proportions; the released E-beta-farnesene not only can directly avoid and keep away aphids but also can enable the aphids to generate uproar to improve the effect of discovery and eating of the aphids by the ladybugs; and the added greenery odor can appropriately adjust the stability of the release of the alarm pheromone and increase the release lasting period of the alarm pheromone so as to achieve an aim of avoiding, keeping away, catching and killing the aphids. A prevention and treatment test proves that by use of the method disclosed by the invention, the prevention and treatment effect, compared with that of separate use of the ladybugs or the pheromone for prevention and treatment, can be improved by 25-45%; and the method disclosed by the invention can reduce the usage amount of chemical pesticides to reduce environmental pollution and enable the aphids to generate drug resistance difficultly, thereby protecting biodiversity and natural enemies resources, and developing a new means of the pheromone in the field of prevention and treatment of pests.

Description

A kind of utilization pheromone complex combines the method for preventing and treating phytophthira pest with ladybug
Technical field
The invention belongs to agricultural insect pest's Prevention Technique field, and in particular to one kind is combined using pheromone complex with ladybug The method of preventing and treating phytophthira pest.
Background technology
Aphid, is all under a class plant-feed insect, including Aphidoidea (also known as aphid Superfamily) also known as aphid, aphid Member.About 4400 kinds of a total of 10 sections of aphid having now been found that, wherein majority belongs to Aphidiadae.Aphid be also on the earth most One of disruptive insect.Wherein about 250 kinds is for agriculture and forestry and horticulture endanger serious insect.Aphid is numerous Grow strong with adaptive faculty, conventional chemical prevention and controls are all difficult the effect for obtaining radical cure, and chemical pesticide is used in a large number, makes agriculture Industry insect generally generates Drug resistance.How to efficiently control it to cause harm, while chemical pesticide environmental pollution is reduced, and to day Enemy's insect infestations, are a problem demanding prompt solutions.With developing rapidly that Chemical ecology of insect is studied, insect pheromone is prevented Control technology to be widely used in the comprehensive control of insect.
Aphid can secret out of thick liquid, i.e. aphid alarm signal when being preyed on or being met with other interference by natural enemy from its cornical Breath element, this Volatile infrochemicals as a kind of chemical communication material, the warning of aphid around, stopping can be caused to take food, It is in a tumult and flees danger the behavior of environment.Bowers etc.（1972）First isolation identification goes out the trans- β-Fa Ni of Aphid Alarm Pheromone Alkene（E- β-farnesene, E β F）.E β F are a kind of sesquiterpenoidss materials, be most aphids alarm pheromones it is main even Sole component（Bowerset al., 1972; Francis et al., 2005）, due to E β F it is action time short and unstable Fixed, be difficult to be applied in agricultural, therefore explore E β F becomes domestic and international study hotspot in Field information technology.Many plants In be rich in E β F compositions, by classify purification can obtain high concentration quintessence oil, such as LabiataeHemizygia petiolataE in plant β F component contents are up to more than 70%（Bruceet al., 2005）；UmbelliferaePimpinella khorasanicaPlant E β F compositions also have higher content in stem, flower and seed（Askariet al., 2013）.E β F are not only present in aphid warning It is also one of many Secondary metabolites in hormone.Although using the high concentration quintessence oil slow release method containing E β F to Semen Pisi sativi AphidAcyrthosiphon pisum（Bruce et al., 2005）, vegetable aphid（Cui et al., 2012b）And wheat aphid（Zhou, 2012）Prevention and control in have certain control action, but this is only avoidance effect of the one-component to aphid, the lasting period is short, Model of action is unstable, to insect do not carry out the control in population quantity, and the present invention uses Aphid Alarm Pheromone, day Enemy, plant Odors of green leaves carries out synergistic mode carries out bionomic control to aphid, serves good control effect.And Yet there are no and Aphid Alarm Pheromone E β F and Odors of green leaves are applied in the relevant report for preventing eliminating aphis to natural enemy ladybug.
The content of the invention
It is an object of the invention to provide a kind of utilization pheromone complex combines the side of preventing and treating phytophthira pest with ladybug Method, is a kind of method for improving ladybug predation aphid effect using the complex containing pheromone and plant Odors of green leaves, using such as Lower technical scheme is realized：
A kind of utilization pheromone complex combines the method for preventing and treating phytophthira pest with ladybug, and its specific implementation step is：Will be slow Release carrier hang among aphid group be higher by effect 2 ~ 5cm of target at, each slow-released carrier spacing 8m ~ 10m；Wooden dipper release will be planted simultaneously In aphid group harm part；Ladybug is set to be preyed in aphid accumulation process.
Described information element complex is by trans-beta-farnesene, cis- 3- hexenols and cis- 3- hexenyl acetates according to mass ratio 4 ~7：2~4：1 ~ 3 composition.
The ladybug is the one kind in harmonia axyridia, Propylaea japonica, coccinella septempunctata or coccinella undecimpanctata；The aphid is cotton One kind in aphid, rhopalosiphum padi, hyalopterus arundinis or turnip aphid.
Described information element complex preparation method be：Each components in certain proportion in pheromone complex is dissolved in into normal hexane In, certain density slow-released carrier solution is configured to, and respectively add the antioxidant 2,6 ditertiary butyl p cresol and purple of 0.5mg Outer smooth adsorbent ESCALOL 567, be encapsulated in it is standby in PVC bag, containing compound in each slow-released carrier The amount of thing is 4mg.
It is a further object to provide methods described ladybug in vegetable or crop is improved is anti-eliminating aphis in effect Application.Aphid warning element is combined into raising ladybug and prevents the slow of effect that eliminate aphis by the present invention with Odors of green leaves with optimal proportion Release carrier；Carry out slow-released carrier and ladybug in crop or vegetable field aphid season occurred frequently to discharge simultaneously, with reach control aphid, The purpose of Protect natural enemies and maintenance bio-diversity.
A kind of utilization pheromone complex that the present invention is provided combines the method for preventing and treating phytophthira pest with ladybug, with aphid Alarm pheromones, plant Odors of green leaves are basis, make slow-released carrier by different ratio, the trans- β-Fa Ni for discharging Alkene not only can directly walk quickly and keep away aphid, can also make it produce disturbance, improve ladybug discovery and take food aphid effect, and the plant for adding Thing Odors of green leaves can be appropriate regulation report to the police element release stability, and increase its release the lasting period, walk quickly and keep away and catch to reach The purpose of killing aphids.Controlling experiment proves, using the present invention in the case of, prevent during than being used alone ladybug or pheromone preventive effect Controlling effect can improve 25%-45%, and the present invention can reduce the usage amount of chemical pesticide, reduce environmental pollution, make aphid be difficult to produce Raw Drug resistance, protects bio-diversity and nature enemy in amount, has opened up new tool of the pheromone in pest control field.
Specific embodiment
The present invention further illustrates the present invention with the following example, but protection scope of the present invention is not limited to following reality Apply example.
Embodiment 1：Laboratory test
1）3 basin cabbage plants of indoor plantation are taken, No. 1 is denoted as, No. 2, No. 3, are all covered cabbage plant with fly net, Experiment is positioned over indoor, 200 aphids for taking indoor feeding are connected on No. 1 basin cabbage plant blade, by 1 female ladybug release On No. 1 cabbage plant blade, the survey result after 24h is taken food.In triplicate, female ladybug is discharged every time must be for test Age is consistent and individuality is more or less the same.Using this result of the test as CK1.
Through investigation 1, No. 2, as a result the Aphed population of No. 3 cabbage plants shows aphid number on No. 1 plant cabbage leaves Measure as 80 ± 9.8, No. 2 cabbage plant blades are 30 ± 6.9, and No. 3 cabbage plant blades are 8 ± 2.3.
Embodiment 2：Laboratory test
1）By trans-beta-farnesene, cis- 3- hexenols and cis- 3- hexenyl acetates according to mass ratio 4:2:1 is dissolved in normal hexane In, certain density slow-released carrier solution is configured to, and respectively add the antioxidant 2,6 ditertiary butyl p cresol and purple of 0.5mg Outer smooth adsorbent ESCALOL 567, is encapsulated in PVC bag, is put in 0-4 DEG C of refrigerator standby.
2）3 basin cabbage plants of indoor plantation are taken, No. 1 is denoted as, No. 2, No. 3, with fly net by cabbage plant whole Cover, be positioned over experiment interior, 200 aphids for taking indoor feeding are connected on No. 1 basin cabbage plant blade, by 1 female wooden dipper Worm is released on No. 1 cabbage plant blade, while the complex slow-released carrier containing report to the police element and Odors of green leaves is hung on into Caulis et Folium Brassicae capitatae At the 3cm of plant top, the survey result after 24h is taken food.In triplicate, it must be age one that female ladybug is discharged every time for test Cause and individuality is more or less the same.
Through investigation 1, No. 2, as a result the Aphed population of No. 3 cabbage plants shows aphid number on No. 1 plant cabbage leaves Measure as 36 ± 8.8, No. 2 cabbage plant blades are 10 ± 2.6, and No. 3 cabbage plant blades are 25 ± 5.8.
Embodiment 3：Laboratory test
1）By trans-beta-farnesene, cis- 3- hexenols and cis- 3- hexenyl acetates according to mass ratio 5:3:2 are dissolved in normal hexane In, certain density slow-released carrier solution is configured to, and respectively add the antioxidant 2,6 ditertiary butyl p cresol and purple of 0.5mg Outer smooth adsorbent ESCALOL 567, is encapsulated in PVC bag, is put in 0-4 DEG C of refrigerator standby.
2）3 basin cabbage plants of indoor plantation are taken, No. 1 is denoted as, No. 2, No. 3, with fly net by cabbage plant whole Cover, be positioned over experiment interior, 200 aphids for taking indoor feeding are connected on No. 1 basin cabbage plant blade, by 1 female wooden dipper Worm is released on No. 1 cabbage plant blade, while the complex slow-released carrier containing report to the police element and Odors of green leaves is hung on into Caulis et Folium Brassicae capitatae At the 3cm of plant top, the survey result after 24h is taken food.In triplicate, it must be age one that female ladybug is discharged every time for test Cause and individuality is more or less the same.
Through investigation 1, No. 2, as a result the Aphed population of No. 3 cabbage plants shows aphid number on No. 1 plant cabbage leaves Measure as 29 ± 7.0, No. 2 cabbage plant blades are 8 ± 1.5, and No. 3 cabbage plant blades are 30 ± 6.4.
Embodiment 4：Laboratory test
1）By trans-beta-farnesene, cis- 3- hexenols and cis- 3- hexenyl acetates according to mass ratio 7:4:3 are dissolved in normal hexane In, certain density slow-released carrier solution is configured to, and respectively add the antioxidant 2,6 ditertiary butyl p cresol and purple of 0.5mg Outer smooth adsorbent ESCALOL 567, is encapsulated in PVC bag, is put in 0-4 DEG C of refrigerator standby.
2）3 basin cabbage plants of indoor plantation are taken, No. 1 is denoted as, No. 2, No. 3, with fly net by cabbage plant whole Cover, be positioned over experiment interior, 200 aphids for taking indoor feeding are connected on No. 1 basin cabbage plant blade, by 1 female wooden dipper Worm is released on No. 1 cabbage plant blade, while the complex slow-released carrier containing report to the police element and Odors of green leaves is hung on into Caulis et Folium Brassicae capitatae At the 3cm of plant top, the survey result after 24h is taken food.In triplicate, it must be age one that female ladybug is discharged every time for test Cause and individuality is more or less the same.
Through investigation 1, No. 2, as a result the Aphed population of No. 3 cabbage plants shows aphid number on No. 1 plant cabbage leaves Measure as 32 ± 7.1, No. 2 cabbage plant blades are 12 ± 2.1, and No. 3 cabbage plant blades are 26 ± 5.5.
Embodiment 5：Field test
1）Aphid season occurred frequently, Fujian Academy Institute of Plant Protection experimental field on select one piece of 100m2Caulis et Folium Brassicae capitatae experimental plot, totally 9 furrow, Per furrow 10, totally 90 Caulis et Folium Brassicae capitataes, 3 furrow are a cell, and it is 3 repetitions to divide 3 cells, is denoted as cell A, B, C, equal conduct CK.Before on-test, the Aphed population in each plot experiment field is first investigated, the Aphed population through investigating cell A is 270 Head, cell B is 330, and cell C is 355.
2）Each female ladybug of cell release, 1 female ladybug is put per 3 Caulis et Folium Brassicae capitataes, and only the first furrow in each cell are released Ladybug is put, remaining 2 furrow does not discharge, female ladybug should be that age is consistent and individuality is more or less the same.Experimental investigation result after 24h.
Borer population is investigated after 24h, is as a result shown, the total aphid number of cell A is 185, the total aphid number of cell B is 245, The total aphid number of cell C is 275.
Embodiment 6：Field test
1）By trans-beta-farnesene, cis- 3- hexenols and cis- 3- hexenyl acetates according to mass ratio 4:2:1 is dissolved in normal hexane In, certain density slow-released carrier solution is configured to, and respectively add the antioxidant 2,6 ditertiary butyl p cresol and purple of 0.5mg Outer smooth adsorbent ESCALOL 567, is encapsulated in PVC bag, is put in 0-4 DEG C of refrigerator standby.
2）In aphid season occurred frequently, at CK30m, one piece of selection is consistent with CK areas, arrangement and Caulis et Folium Brassicae capitatae quantity 100m2Caulis et Folium Brassicae capitatae experimental plot as process, totally 9 furrow, per furrow 10, totally 90 Caulis et Folium Brassicae capitataes, 3 furrow are a cell, divide 3 cells and are 3 repetitions, are denoted as cell A1, B1, C1.Before on-test, the Aphed population in each plot experiment field is first investigated, through adjusting Look into cell A1Aphed population be 280, cell B1For 340, cell C1For 360.
3）Two slow-released carriers, spacing 5m, the 3cm at cabbage leaves, while release are hung in each furrow of cell first Female ladybug, 1 female ladybug is put per 3 Caulis et Folium Brassicae capitataes, and only the first furrow in each cell discharge ladybug, and remaining 2 furrow does not discharge, female Property ladybug should be that age is consistent and individuality is more or less the same.Experimental investigation result after 24h.
Borer population is investigated after 24h, is as a result shown, cell A1Total aphid number is 150, cell B1Total aphid number is 190 Head, cell C1Total aphid number is 200.
Embodiment 7：Field test
1）By trans-beta-farnesene, cis- 3- hexenols and cis- 3- hexenyl acetates according to mass ratio 5:3:2 are dissolved in normal hexane In, certain density slow-released carrier solution is configured to, and respectively add the antioxidant 2,6 ditertiary butyl p cresol and purple of 0.5mg Outer smooth adsorbent ESCALOL 567, is encapsulated in PVC bag, is put in 0-4 DEG C of refrigerator standby.
2）In aphid season occurred frequently, at CK45m, one piece of selection is consistent with CK areas, arrangement and Caulis et Folium Brassicae capitatae quantity 100m2Caulis et Folium Brassicae capitatae experimental plot as process, totally 9 furrow, per furrow 10, totally 90 Caulis et Folium Brassicae capitataes, 3 furrow are a cell, divide 3 cells and are 3 repetitions, are denoted as cell A2, B2, C2.Before on-test, the Aphed population in each plot experiment field is first investigated, through adjusting Look into cell A2Aphed population be 280, cell B2For 350, cell C2For 320.
3）Two slow-released carriers, spacing 5m, the 3cm at cabbage leaves, while release are hung in each furrow of cell first Female ladybug, 1 female ladybug is put per 3 Caulis et Folium Brassicae capitataes, and only the first furrow in each cell discharge ladybug, and remaining 2 furrow does not discharge, female Property ladybug should be that age is consistent and individuality is more or less the same.Experimental investigation result after 24h.
Borer population is investigated after 24h, is as a result shown, cell A2Total aphid number is 145, cell B2Total aphid number is 195 Head, cell C2Total aphid number is 180.
Embodiment 8：Field test
1）By trans-beta-farnesene, cis- 3- hexenols and cis- 3- hexenyl acetates according to mass ratio 7:4:3 are dissolved in normal hexane In, certain density slow-released carrier solution is configured to, and respectively add the antioxidant 2,6 ditertiary butyl p cresol and purple of 0.5mg Outer smooth adsorbent ESCALOL 567, is encapsulated in PVC bag, is put in 0-4 DEG C of refrigerator standby.
2）In aphid season occurred frequently, at CK60m, one piece of selection is consistent with CK areas, arrangement and Caulis et Folium Brassicae capitatae quantity 100m2Caulis et Folium Brassicae capitatae experimental plot as process, totally 9 furrow, per furrow 10, totally 90 Caulis et Folium Brassicae capitataes, 3 furrow are a cell, divide 3 cells and are 3 repetitions, are denoted as cell A3, B3, C3.Before on-test, the Aphed population in each plot experiment field is first investigated, through adjusting Look into cell A3Aphed population be 305, cell B3For 320, cell C3For 310.
3）Two slow-released carriers, spacing 5m, the 3cm at cabbage leaves, while release are hung in each furrow of cell first Female ladybug, 1 female ladybug is put per 3 Caulis et Folium Brassicae capitataes, and only the first furrow in each cell discharge ladybug, and remaining 2 furrow does not discharge, female Property ladybug should be that age is consistent and individuality is more or less the same.Experimental investigation result after 24h.
Borer population is investigated after 24h, is as a result shown, cell A3Total aphid number is 145, cell B3Total aphid number is 185 Head, cell C3Total aphid number is 180.
To sum up result shows that when testing indoors, it is 118 that ladybug takes food CK aphids total amount after 24h；Hanging containing warning Aphid total amount is 70 or so after the complex slow-released carrier of element and plant Odors of green leaves, is significantly reduced compared with CK, and aphid is all It is gathered in from slow-released carrier larger distance.In field test, hang the complex containing report to the police element and plant Odors of green leaves and delay Release aphid total amount after carrier also to significantly reduce compared with CK, and aphid is followed successively by from less to more the 3rd in the distributed quantity of each cell Furrow, the 2nd furrow, the 1st furrow.Controlling experiment proves, using the present invention in the case of, during than being used alone ladybug or pheromone preventive effect Prevention effect can improve 25%-45%.

Claims (5)

1. a kind of method that utilization pheromone complex combines preventing and treating phytophthira pest with ladybug, it is characterised in that its concrete reality Applying step is：Slow-released carrier is hung among aphid group and is higher by effect 2 ~ 5cm of target, each slow-released carrier spacing 8m ~ 10m； Ladybug is released among aphid simultaneously；Ladybug is set to be preyed in aphid accumulation process.
2. the method that a kind of utilization pheromone complex as claimed in claim 1 combines preventing and treating phytophthira pest with ladybug, its It is characterised by, described information element complex is by trans-beta-farnesene, cis- 3- hexenols and cis- 3- hexenyl acetates according to mass ratio 4~7：2~4：1 ~ 3 composition.
3. the method that a kind of utilization pheromone complex as claimed in claim 1 combines preventing and treating phytophthira pest with ladybug, its It is characterised by, the ladybug is the one kind in harmonia axyridia, Propylaea japonica, coccinella septempunctata or coccinella undecimpanctata；The aphid is One kind in cotten aphid, rhopalosiphum padi, hyalopterus arundinis or turnip aphid.
4. the method that a kind of utilization pheromone complex as claimed in claim 1 combines preventing and treating phytophthira pest with ladybug, its It is characterised by, the preparation method of described information element complex is：By each components in certain proportion in pheromone complex be dissolved in just oneself In alkane, be configured to certain density slow-released carrier solution, and respectively add 0.5mg antioxidant 2,6 ditertiary butyl p cresol and Ultraviolet light adsorbent ESCALOL 567, be encapsulated in it is standby in PVC bag, containing multiple in each slow-released carrier The amount of compound is 4mg.
5. the method for combining preventing and treating phytophthira pest with ladybug using a kind of utilization pheromone complex described in claim 1 exists Improve ladybug on vegetable or crop and prevent the application eliminated aphis in effect.
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CN108967423A (en) * 2018-09-12 2018-12-11 南京新安中绿生物科技有限公司 Pest mating is interfered to attract sustained release preparation and the application of beneficial insect
CN109757265A (en) * 2019-03-12 2019-05-17 汶川德新果缘农业有限公司 The pest control method in natural enemy insect mixing field
CN109769572A (en) * 2019-03-22 2019-05-21 广西壮族自治区林业科学研究院 A method of combining the loose tip pest of prevention and treatment with insect parasite using trapping
CN113207832A (en) * 2021-05-13 2021-08-06 杭州茶菊科技有限公司 Method for push-pull type trapping and killing winged tea aphids in growing season by using repellent and attractant
CN113748902A (en) * 2021-10-09 2021-12-07 安徽南北现代林业科技有限公司 Method for removing overwintering aphids of goldenrain tree

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1631091A (en) * 2005-01-26 2005-06-29 北京市农林科学院 Biological control method for orchard ecology
CN102498985A (en) * 2011-11-03 2012-06-20 福建省农业科学院植物保护研究所 Method for preventing and controlling green soy bean bemisia tabaci by utilizing combination of Japanese serangium japonicum and trap crop
CN103238591A (en) * 2013-05-02 2013-08-14 中国农业大学 Micro-emulsion with aphid alarm pheromone analogue and preparation method for micro-emulsion
CN103858869A (en) * 2014-03-10 2014-06-18 中国计量学院 Method for luring Chrysopa sinica Tjeder to restrict Toxoptera aurantii Boyer by pheromones
CN104094930A (en) * 2014-07-23 2014-10-15 山东农业大学 Aphid behavior regulating and controlling sustained-release agent and preparation method thereof
CN104430541A (en) * 2013-09-15 2015-03-25 苏州研迪智能科技有限公司 Method for pest trapping with insect pheromone
CN104585137A (en) * 2014-12-31 2015-05-06 红塔烟草(集团)有限责任公司 Method for preventing and curing tobacco pests by ladybird breeding via fennel aphids in field
CN104938263A (en) * 2015-07-20 2015-09-30 胡永军 Method for controlling greenhouse vegetable aphids through adoption of harmonia axyridis

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1631091A (en) * 2005-01-26 2005-06-29 北京市农林科学院 Biological control method for orchard ecology
CN102498985A (en) * 2011-11-03 2012-06-20 福建省农业科学院植物保护研究所 Method for preventing and controlling green soy bean bemisia tabaci by utilizing combination of Japanese serangium japonicum and trap crop
CN103238591A (en) * 2013-05-02 2013-08-14 中国农业大学 Micro-emulsion with aphid alarm pheromone analogue and preparation method for micro-emulsion
CN104430541A (en) * 2013-09-15 2015-03-25 苏州研迪智能科技有限公司 Method for pest trapping with insect pheromone
CN103858869A (en) * 2014-03-10 2014-06-18 中国计量学院 Method for luring Chrysopa sinica Tjeder to restrict Toxoptera aurantii Boyer by pheromones
CN104094930A (en) * 2014-07-23 2014-10-15 山东农业大学 Aphid behavior regulating and controlling sustained-release agent and preparation method thereof
CN104585137A (en) * 2014-12-31 2015-05-06 红塔烟草(集团)有限责任公司 Method for preventing and curing tobacco pests by ladybird breeding via fennel aphids in field
CN104938263A (en) * 2015-07-20 2015-09-30 胡永军 Method for controlling greenhouse vegetable aphids through adoption of harmonia axyridis

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
尹松雷: "昆虫信息化合物在现代社会害虫防治中的优越性及其应用", 《化学教育》 *
崔宏春等: "昆虫信息化合物在茶园害虫防治中的应用进展", 《安徽农业科学》 *
张勇: "麦长管蚜对一种植物挥发物及蚜虫报警信息素的嗅觉行为检测", 《中国植物保护学会成立50周年庆祝大会暨2012年学术年会论文集》 *
曾春等: "信息化合物对昆虫行为的影响及其在生物防治中的应用", 《江西植保》 *
柴全喜等: "春季果树蚜虫的综合防治", 《河北农民报》 *
范佳等: "小麦与蔬菜蚜虫新型防控技术研究进展", 《应用昆虫学报》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107711836A (en) * 2017-11-13 2018-02-23 中国农业科学院植物保护研究所 A kind of wasp fly traps composition and its application
CN108967423A (en) * 2018-09-12 2018-12-11 南京新安中绿生物科技有限公司 Pest mating is interfered to attract sustained release preparation and the application of beneficial insect
CN108967423B (en) * 2018-09-12 2021-11-12 南京新安中绿生物科技有限公司 Slow-release preparation for interfering mating of pests and attracting beneficial insects and application
CN109757265A (en) * 2019-03-12 2019-05-17 汶川德新果缘农业有限公司 The pest control method in natural enemy insect mixing field
CN109769572A (en) * 2019-03-22 2019-05-21 广西壮族自治区林业科学研究院 A method of combining the loose tip pest of prevention and treatment with insect parasite using trapping
CN113207832A (en) * 2021-05-13 2021-08-06 杭州茶菊科技有限公司 Method for push-pull type trapping and killing winged tea aphids in growing season by using repellent and attractant
CN113748902A (en) * 2021-10-09 2021-12-07 安徽南北现代林业科技有限公司 Method for removing overwintering aphids of goldenrain tree

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