CN106561359B - A method of combining prevention and treatment phytophthira pest with ladybug using pheromones compound - Google Patents
A method of combining prevention and treatment phytophthira pest with ladybug using pheromones compound Download PDFInfo
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- CN106561359B CN106561359B CN201611017469.1A CN201611017469A CN106561359B CN 106561359 B CN106561359 B CN 106561359B CN 201611017469 A CN201611017469 A CN 201611017469A CN 106561359 B CN106561359 B CN 106561359B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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Abstract
The invention discloses a kind of methods using pheromones compound and ladybug joint prevention and treatment phytophthira pest, using Aphid Alarm Pheromone, plant Odors of green leaves as basis, slow-released carrier is made by different ratio, the trans-beta-farnesene released not only can directly walk quickly and keep away aphid, it can be also set to generate disturbance, improve ladybug discovery and feeding aphid effect, and the plant Odors of green leaves being added appropriate can adjust the stability of alarm element release, and increase it and discharge the lasting period, to achieve the purpose that walk quickly and keep away and catch and kill aphid.Controlling experiment proves; in the case where using the present invention; 25%-45% can be improved in control efficiency when than ladybug or pheromones preventive effect is used alone; the present invention can reduce the usage amount of chemical pesticide; it reduces environmental pollution; so that aphid is not likely to produce drug resistance, protect bio-diversity and nature enemy in amount, opens up pheromones in the new tool in pest control field.
Description
Technical field
The invention belongs to agricultural insect pest's Prevention Technique fields, and in particular to a kind of to be combined using pheromones compound with ladybug
The method for preventing and treating phytophthira pest.
Background technique
Aphid, also known as aphid, aphid are a kind of plant-feed insects, including all under Aphidoidea (also known as aphid Superfamily)
Member.About 4400 kinds of a total of 10 sections of the aphid having now been found that, wherein majority belongs to Aphidiadae.Aphid be also on the earth most
One of disruptive pest.Wherein about 250 kinds are to endanger serious pest for agriculture and forestry and horticulture.Aphid is numerous
Grow strong with adaptive faculty, conventional chemical control method is all difficult to obtain the effect of radical cure, and a large amount of uses of chemical pesticide make agriculture
Industry pest generally produces drug resistance.How to efficiently control it to cause harm, while reducing chemical pesticide environmental pollution, and to day
Insect infestations are opposed, are a urgent problems to be solved.With the rapid development that Chemical ecology of insect is studied, insect pheromone is anti-
Technology is controlled to be widely used in the comprehensive treatment of pest.
Aphid can secret out of thick liquid, i.e. aphid alarm signal when being preyed on by natural enemy or meeting with other interference from its cornical
Breath element, this Volatile infrochemicals as a kind of chemical communication substance, can cause the warning of aphid around, stop feeding,
It is in a tumult and flees danger the behavior of environment.Bowers etc. (1972) is separated identify the trans- β-Fa Ni of Aphid Alarm Pheromone for the first time
Alkene (E- β-farnesene, E β F).E β F is a kind of sesquiterpenoids substance, be most aphids alarm pheromones it is main even
Sole component (Bowerset al., 1972; Francis et al., 2005), since E β F action time is short and unstable
It is fixed, it is difficult to be applied in agricultural, therefore explore E β F to become domestic and international research hotspot in Field information technology.Many plants
In be rich in E β F ingredient, pass through classification purify can get high concentration essential oil, such as LabiataeHemizygia petiolataE in plant
β F component content is up to 70% or more (Bruceet al., 2005)；UmbelliferaePimpinella khorasanicaPlant
E β F ingredient also has higher content (Askari in stem, flower and seedet al., 2013).E β F is not only present in aphid alarm
In hormone and one of many Secondary metabolites.Although using the high concentration essential oil slow release method containing E β F to pea
AphidAcyrthosiphon pisum(Bruce et al., 2005), vegetable aphid (Cui et al., 2012b) and wheat aphid (Zhou,
2012) there is certain control action in prevention and control, but this is only avoidance effect of the one-component to aphid, the lasting period is short,
The mode of action is unstable, does not carry out the control in population quantity to pest, and the present invention is using Aphid Alarm Pheromone, day
The mode that enemy, plant Odors of green leaves act synergistically carries out bionomic control to aphid, plays good control effect.And
It yet there are no and apply Aphid Alarm Pheromone E β F and Odors of green leaves to the anti-relevant report to eliminate aphis of natural enemy ladybug.
Summary of the invention
The purpose of the present invention is to provide a kind of to combine the side for preventing and treating phytophthira pest using pheromones compound with ladybug
Method is a kind of using the method for improving ladybug predation aphid effect containing the compound of pheromones and plant Odors of green leaves, using such as
Lower technical solution is realized:
A method of combining prevention and treatment phytophthira pest, specific implementation step with ladybug using pheromones compound are as follows:
Slow-released carrier is hung among aphid group and is higher by effect 2 ~ 5cm of target, each slow-released carrier spacing 8m ~ 10m；Wooden dipper will be planted simultaneously
It is released to aphid group's harm place；Prey on ladybug in aphid accumulation process.
The pheromones compound is by trans-beta-farnesene, cis- 3- hexenol and cis- 3- hexenyl acetate according to mass ratio 4
~ 7:2 ~ 4:1 ~ 3 is formed.
The ladybug is one of harmonia axyridia, Propylaea japonica, coccinella septempunctata or coccinella undecimpanctata；The aphid is cotton
One of aphid, rhopalosiphum padi, hyalopterus arundinis or turnip aphid.
The pheromones compound the preparation method comprises the following steps: components in certain proportion each in pheromones compound is dissolved in n-hexane
In, it is configured to certain density slow-released carrier solution, and the antioxidant 2,6-di-tert-butyl p-cresol and purple of 0.5mg is respectively added
Outer smooth adsorbent ESCALOL 567, be encapsulated in it is spare in PVC bag, containing compound in each slow-released carrier
The amount of object is 4mg.
It is a further object to provide the methods in improving vegetables or crop in the anti-effect that eliminates aphis of ladybug
Application.Aphid alarm element and Odors of green leaves are combined into optimal proportion and improve the slow of the anti-effect that eliminates aphis of ladybug by the present invention
Release carrier；Carry out slow-released carrier in crop or vegetables field aphid high-incidence season and ladybug discharge simultaneously, with reach control aphid,
Protect natural enemies and the purpose for maintaining bio-diversity.
A kind of method for combining prevention and treatment phytophthira pest with ladybug using pheromones compound provided by the invention, with aphid
Alarm pheromones, plant Odors of green leaves are basis, slow-released carrier are made by different ratio, the trans- β-Fa Ni released
Alkene not only can directly walk quickly and keep away aphid, so that it is generated disturbance, raising ladybug finds and feeding aphid effect, and the plant being added
Object Odors of green leaves appropriate can adjust the stability of alarm element release, and increase it and discharge the lasting period, walk quickly and keep away and catch to reach
The purpose of killing aphids.Controlling experiment proves, in the case where using the present invention, prevents when than ladybug or pheromones preventive effect is used alone
Controlling effect can be improved 25%-45%, and the present invention can reduce the usage amount of chemical pesticide, reduce environmental pollution, and aphid is made to be not easy to produce
Raw drug resistance, protects bio-diversity and nature enemy in amount, has opened up pheromones in the new tool in pest control field.
Specific embodiment
The present invention is further illustrated by the following examples, but protection scope of the present invention is not limited to following reality
Apply example.
Embodiment 1: laboratory test
1) the 3 basin cabbage plants for taking indoor plantation, are denoted as No. 1, and No. 2, No. 3, with fly net by cabbage plant whole
It covers, is placed in laboratory, 200 aphids of indoor raising is taken to be connected on No. 1 basin cabbage plant blade, by 1 female wooden dipper
Worm is released on No. 1 cabbage plant blade, the investigation result after feeding for 24 hours.Test in triplicate, discharges female ladybug every time
It must be that age is consistent and individual is not much different.Using this test result as CK1.
By investigation No. 1, No. 2, the Aphed population of No. 3 cabbage plants, the results showed that aphid number on No. 1 plant cabbage leaves
Amount is 80 ± 9.8, and No. 2 cabbage plant blades are 30 ± 6.9, and No. 3 cabbage plant blades are 8 ± 2.3.
Embodiment 2: laboratory test
1) by trans-beta-farnesene, cis- 3- hexenol and cis- 3- hexenyl acetate according to mass ratio 4:2:1 be dissolved in just oneself
In alkane, be configured to certain density slow-released carrier solution, and respectively be added 0.5mg antioxidant 2,6-di-tert-butyl p-cresol and
Ultraviolet light adsorbent ESCALOL 567, is encapsulated in PVC bag, and it is spare to be put in 0-4 DEG C of refrigerator.
2) the 3 basin cabbage plants for taking indoor plantation, are denoted as No. 1, and No. 2, No. 3, with fly net by cabbage plant whole
It covers, is placed in laboratory, 200 aphids of indoor raising is taken to be connected on No. 1 basin cabbage plant blade, by 1 female wooden dipper
Worm is released on No. 1 cabbage plant blade, while the compound slow-released carrier of element containing alarm and Odors of green leaves is hung on wild cabbage
Above plant at 3cm, the investigation result after feeding for 24 hours.In triplicate, discharging female ladybug every time must be age one for test
It causes and individual is not much different.
By investigation No. 1, No. 2, the Aphed population of No. 3 cabbage plants, the results showed that aphid number on No. 1 plant cabbage leaves
Amount is 36 ± 8.8, and No. 2 cabbage plant blades are 10 ± 2.6, and No. 3 cabbage plant blades are 25 ± 5.8.
Embodiment 3: laboratory test
1) by trans-beta-farnesene, cis- 3- hexenol and cis- 3- hexenyl acetate according to mass ratio 5:3:2 be dissolved in just oneself
In alkane, be configured to certain density slow-released carrier solution, and respectively be added 0.5mg antioxidant 2,6-di-tert-butyl p-cresol and
Ultraviolet light adsorbent ESCALOL 567, is encapsulated in PVC bag, and it is spare to be put in 0-4 DEG C of refrigerator.
2) the 3 basin cabbage plants for taking indoor plantation, are denoted as No. 1, and No. 2, No. 3, with fly net by cabbage plant whole
It covers, is placed in laboratory, 200 aphids of indoor raising is taken to be connected on No. 1 basin cabbage plant blade, by 1 female wooden dipper
Worm is released on No. 1 cabbage plant blade, while the compound slow-released carrier of element containing alarm and Odors of green leaves is hung on wild cabbage
Above plant at 3cm, the investigation result after feeding for 24 hours.In triplicate, discharging female ladybug every time must be age one for test
It causes and individual is not much different.
By investigation No. 1, No. 2, the Aphed population of No. 3 cabbage plants, the results showed that aphid number on No. 1 plant cabbage leaves
Amount is 29 ± 7.0, and No. 2 cabbage plant blades are 8 ± 1.5, and No. 3 cabbage plant blades are 30 ± 6.4.
Embodiment 4: laboratory test
1) by trans-beta-farnesene, cis- 3- hexenol and cis- 3- hexenyl acetate according to mass ratio 7:4:3 be dissolved in just oneself
In alkane, be configured to certain density slow-released carrier solution, and respectively be added 0.5mg antioxidant 2,6-di-tert-butyl p-cresol and
Ultraviolet light adsorbent ESCALOL 567, is encapsulated in PVC bag, and it is spare to be put in 0-4 DEG C of refrigerator.
2) the 3 basin cabbage plants for taking indoor plantation, are denoted as No. 1, and No. 2, No. 3, with fly net by cabbage plant whole
It covers, is placed in laboratory, 200 aphids of indoor raising is taken to be connected on No. 1 basin cabbage plant blade, by 1 female wooden dipper
Worm is released on No. 1 cabbage plant blade, while the compound slow-released carrier of element containing alarm and Odors of green leaves is hung on wild cabbage
Above plant at 3cm, the investigation result after feeding for 24 hours.In triplicate, discharging female ladybug every time must be age one for test
It causes and individual is not much different.
By investigation No. 1, No. 2, the Aphed population of No. 3 cabbage plants, the results showed that aphid number on No. 1 plant cabbage leaves
Amount is 32 ± 7.1, and No. 2 cabbage plant blades are 12 ± 2.1, and No. 3 cabbage plant blades are 26 ± 5.5.
Embodiment 5: field trial
1) aphid high-incidence season experimental field above selects one piece of 100m in Fujian Academy Institute of Plant Protection2Wild cabbage experimental plot, altogether
9 furrow, every furrow 10, totally 90 wild cabbages, 3 furrow are a cell, divide 3 cells i.e. 3 repetitions, are denoted as cell A, B, C, make
For CK.Before on-test, the Aphed population in each plot experiment field is first investigated, the Aphed population by investigating cell A is
270, cell B is 330, and cell C is 355.
2) each cell discharges female ladybug, and every 3 wild cabbages are put 1 female ladybug, only released in the first furrow of each cell
Ladybug is put, remaining 2 furrow does not discharge, and female ladybug should be that age is consistent and individual is not much different.Experimental investigation result after for 24 hours.
Investigate borer population afterwards for 24 hours, the results showed that, cell A total aphid number is 185, and cell B total aphid number is 245,
Cell C total aphid number is 275.
Embodiment 6: field trial
1) by trans-beta-farnesene, cis- 3- hexenol and cis- 3- hexenyl acetate according to mass ratio 4:2:1 be dissolved in just oneself
In alkane, be configured to certain density slow-released carrier solution, and respectively be added 0.5mg antioxidant 2,6-di-tert-butyl p-cresol and
Ultraviolet light adsorbent ESCALOL 567, is encapsulated in PVC bag, and it is spare to be put in 0-4 DEG C of refrigerator.
2) aphid high-incidence season, at distance CK30m, one piece of selection is consistent with CK area, arrangement and wild cabbage quantity
100m2Wild cabbage experimental plot is used as processing, totally 9 furrow, every furrow 10, totally 90 wild cabbages, and 3 furrow are a cell, divides 3 cells i.e.
3 repetitions, are denoted as cell A1, B1, C1.Before on-test, the Aphed population in each plot experiment field is first investigated, through toning
Look into cell A1Aphed population be 280, cell B1It is 340, cell C1It is 360.
3) two slow-released carriers are hung in each the first furrow of cell, spacing 5m, the 3cm at cabbage leaves discharge simultaneously
Female ladybug, every 3 wild cabbages put 1 female ladybug, only discharge ladybug in the first furrow of each cell, remaining 2 furrow does not discharge, female
Property ladybug should be that age is consistent and individual is not much different.Experimental investigation result after for 24 hours.
Investigate borer population afterwards for 24 hours, the results showed that, cell A1Total aphid number is 150, cell B1Total aphid number is 190
Head, cell C1Total aphid number is 200.
Embodiment 7: field trial
1) by trans-beta-farnesene, cis- 3- hexenol and cis- 3- hexenyl acetate according to mass ratio 5:3:2 be dissolved in just oneself
In alkane, be configured to certain density slow-released carrier solution, and respectively be added 0.5mg antioxidant 2,6-di-tert-butyl p-cresol and
Ultraviolet light adsorbent ESCALOL 567, is encapsulated in PVC bag, and it is spare to be put in 0-4 DEG C of refrigerator.
2) aphid high-incidence season, at distance CK45m, one piece of selection is consistent with CK area, arrangement and wild cabbage quantity
100m2Wild cabbage experimental plot is used as processing, totally 9 furrow, every furrow 10, totally 90 wild cabbages, and 3 furrow are a cell, divides 3 cells i.e.
3 repetitions, are denoted as cell A2, B2, C2.Before on-test, the Aphed population in each plot experiment field is first investigated, through toning
Look into cell A2Aphed population be 280, cell B2It is 350, cell C2It is 320.
3) two slow-released carriers are hung in each the first furrow of cell, spacing 5m, the 3cm at cabbage leaves discharge simultaneously
Female ladybug, every 3 wild cabbages put 1 female ladybug, only discharge ladybug in the first furrow of each cell, remaining 2 furrow does not discharge, female
Property ladybug should be that age is consistent and individual is not much different.Experimental investigation result after for 24 hours.
Investigate borer population afterwards for 24 hours, the results showed that, cell A2Total aphid number is 145, cell B2Total aphid number is 195
Head, cell C2Total aphid number is 180.
Embodiment 8: field trial
1) by trans-beta-farnesene, cis- 3- hexenol and cis- 3- hexenyl acetate according to mass ratio 7:4:3 be dissolved in just oneself
In alkane, be configured to certain density slow-released carrier solution, and respectively be added 0.5mg antioxidant 2,6-di-tert-butyl p-cresol and
Ultraviolet light adsorbent ESCALOL 567, is encapsulated in PVC bag, and it is spare to be put in 0-4 DEG C of refrigerator.
2) aphid high-incidence season, at distance CK60m, one piece of selection is consistent with CK area, arrangement and wild cabbage quantity
100m2Wild cabbage experimental plot is used as processing, totally 9 furrow, every furrow 10, totally 90 wild cabbages, and 3 furrow are a cell, divides 3 cells i.e.
3 repetitions, are denoted as cell A3, B3, C3.Before on-test, the Aphed population in each plot experiment field is first investigated, through toning
Look into cell A3Aphed population be 305, cell B3It is 320, cell C3It is 310.
3) two slow-released carriers are hung in each the first furrow of cell, spacing 5m, the 3cm at cabbage leaves discharge simultaneously
Female ladybug, every 3 wild cabbages put 1 female ladybug, only discharge ladybug in the first furrow of each cell, remaining 2 furrow does not discharge, female
Property ladybug should be that age is consistent and individual is not much different.Experimental investigation result after for 24 hours.
Investigate borer population afterwards for 24 hours, the results showed that, cell A3Total aphid number is 145, cell B3Total aphid number is 185
Head, cell C3Total aphid number is 180.
To sum up the result shows that, during the indoor test, CK aphid total amount is 118 after ladybug feeding for 24 hours；In suspension containing alarm
Aphid total amount is 70 or so after the compound slow-released carrier of element and plant Odors of green leaves, is significantly reduced compared with CK, and aphid is all
It is gathered in from slow-released carrier larger distance.In field trial, the compound for hanging element containing alarm and plant Odors of green leaves is slow
Releasing after carrier aphid total amount also has significant decrease compared with CK, and aphid is followed successively by the 3rd in the distributed quantity of each cell from less to more
Furrow, the 2nd furrow, the 1st furrow.Controlling experiment proves, in the case where using the present invention, when than ladybug or pheromones preventive effect is used alone
25%-45% can be improved in control efficiency.
Claims (2)
1. a kind of method for combining prevention and treatment phytophthira pest with ladybug using pheromones compound, which is characterized in that it is specific real
Apply step are as follows: slow-released carrier is hung among aphid group and is higher by effect 2 ~ 5cm of target, each slow-released carrier spacing 8m ~ 10m；
Ladybug is released among aphid simultaneously；Prey on ladybug in aphid accumulation process；
The pheromones compound is by trans-beta-farnesene, cis- 3- hexenol and cis- 3- hexenyl acetate according to mass ratio 4:2:1
Or 5:3:2 or 7:4:3 composition；
The slow-released carrier the preparation method comprises the following steps: components in certain proportion each in pheromones compound is dissolved in n-hexane, prepare
At certain density slow-released carrier solution, and the antioxidant 2,6-di-tert-butyl p-cresol and ultraviolet light suction of 0.5mg is respectively added
Attached dose of ESCALOL 567, be encapsulated in it is spare in PVC bag, in each slow-released carrier containing pheromones it is compound
The amount of object is 4mg.
2. being existed using a kind of method for combining prevention and treatment phytophthira pest with ladybug using pheromones compound described in claim 1
Improve the application in the anti-effect that eliminates aphis of ladybug on vegetables.
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| CN113207832B (en) * | 2021-05-13 | 2023-03-17 | 杭州茶菊科技有限公司 | Method for push-pull type trapping and killing winged tea aphids in growing season by using repellent and attractant |
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