CN105076214A - Method for improving control effect of entomopathogenic nematodes on Chinese chive maggots - Google Patents
Method for improving control effect of entomopathogenic nematodes on Chinese chive maggots Download PDFInfo
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- CN105076214A CN105076214A CN201510608803.XA CN201510608803A CN105076214A CN 105076214 A CN105076214 A CN 105076214A CN 201510608803 A CN201510608803 A CN 201510608803A CN 105076214 A CN105076214 A CN 105076214A
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Abstract
The invention relates to a method for improving a control effect of entomopathogenic nematodes on Chinese chive maggots. The method comprises the steps that thiamethoxam diluent is mixed with entomopathogenic nematodes which accord with detection conditions to prepare synergistic suspension, the concentration of the nematodes in the synergistic suspension ranges from 500 IJS/mL to 1000 IJS/mL, and the concentration of thiamethoxam ranges from 12 mg/L to 15 mg/L; the synergistic suspension is applied to killing of Chinese chive maggot larva when the Chinese chive maggots are in the larval phase, and normal field management is conducted after application is conducted; under field conditions, the method of adding low-concentration thiamethoxam for utilizing the entomopathogenic nematodes to control the Chinese chive maggots is adopted, the control effect of the entomopathogenic nematodes on the Chinese chive maggots of vegetables such as Chinese chives, green Chinese onions, shallots and garlic which are prone to being damaged by the Chinese chive maggots can be significantly improved, an obviously synergistic effect of the nematodes and the thiamethoxam on control of the Chinese chive maggots is embodied, usage amount of the thiamethoxam and other pesticides are significantly decreased, the baiting technique of a black sticky trap on imagoes is combined, the initial population number of a field is effectively decreased, and the purpose of controlling the Chinese chive maggots in a safe and nuisanceless mode can be really achieved.
Description
Technical field
The present invention relates to a kind of method improving entomopathogenic nematode control fragrant-flowered garlic maggot effect, belong to technical field of biological control.
Background technology
Bradysia odoriphaga is the important pests of harm leek, the liliaceous vegetables such as main harm leek, shallot, onion, spring onion, garlic.Its larva (being commonly called as fragrant-flowered garlic maggot) is mainly clustered in vegetables root, the moth false stem of food and bulb and cause dead seedling.There are for 4 ~ 6 generations at North China open country in this worm, generation overlap serious every year, with larva in leek bulb or around fragrant-flowered garlic root the dormancy of 3 ~ 4cm topsoil survive the winter, spring, season in autumn two occur the heaviest.Due in the cultivation of protecting field leek, temperature and humidity conditions is applicable to growing of fragrant-flowered garlic maggot, therefore it still can continue to endanger leek.
At present, in production, control is still based on chemical insecticide, but effect is not ideal enough, and easily causes environmental pollution, particularly uses phoxim, Imidacloprid and chlopyrifos etc. to carry out filling root.Diacloden is a kind of anabasine insecticide with excellent activity, alternative suppression insect CNS nicotinic acetylcholine esterase acceptor, and then blocks the normal conduction of insect CNS, thus causes insect dead.Not only have tag, stomach toxicity, systemic activity, and there is better safety and the feature such as speed of action is fast, the lasting period is long, be replace that those are high to mammalian toxicity, have residual and the organic phosphor of serious environment pollution, carbamate, organochlorine insecticides better medicament, be widely used in the crops various pests such as control paddy rice, soybean, fruit, vegetables and tealeaves.Chlopyrifos is a kind of efficient, broad-spectrum organophosphorous pesticide, have insect tag, stomach toxicity and fumigation action, belong to poisoning insecticide, to fish and aquatic toxicity higher, poisonous to honeybee, on blade, residual life, is generally 5 ~ 7 days, and in soil, residual life is longer.Beta-cypermethrin is a kind of pyrethroid insecticides, and biologically active is higher, is the efficient isomer of cypermethrin, has and tag and stomach poison function.Insecticidal spectrum is wide, speed of knocking down is fast, and insecticidal activity comparatively cypermethrin is high.Be applicable to prevent and treat the insect in the various plants such as cotton, vegetables, fruit tree, tea tree, forest and sanitary insect pest.
High malicious chemical insecticide or increasing insecticide amount of application control fragrant-flowered garlic maggot, not only cause its pesticide resistance to increase substantially, also have a strong impact on the edible quality of crop, even occur malicious fragrant-flowered garlic, malicious garlic and malicious green onion etc.Therefore, the nuisanceless technology prevention fragrant-flowered garlic maggot of application is needed badly.Entomopathogenic nematode is the nematode of the special parasitic insect of a class, entered in pin main body by the mode of 3 infective stage larvas in age to the natural opening of insect health or intersegmental membrane intrusion, and discharge the symbiotic bacteria of carrying, make it breed in pest body, produce toxin, finally cause insect dead.Because of entomopathogenic nematode, to have host range extensive, initiatively find host, to non-target organism safety, environment is had no side effect, can be mixed with many insecticides, fertilizer etc. advantage, be widely used in the multiple agriculture and forestry injurious insects such as control of grubs, cutworm, peach fruit borer, longicorn and fragrant-flowered garlic maggot.But compared with chemical insecticide, entomopathogenic nematode insecticidal effect is slow and unstable.In application nematoda control fragrant-flowered garlic maggot, though early-stage Study can prove that nematode has certain control efficiency to fragrant-flowered garlic maggot, but nematode dosage used is higher, and insecticidal effect is lower, cannot reach the object controlling fragrant-flowered garlic maggot.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of method improving entomopathogenic nematode control fragrant-flowered garlic maggot effect.
Summary of the invention:
The present invention improves the method for entomopathogenic nematode control fragrant-flowered garlic maggot effect, slow and unstable for entomopathogenic nematode control fragrant-flowered garlic maggot larva effect, pest control with insecticide is poisonous, and at present technical problem that is rampant and the novel pesticide control of shortage high-efficiency low-toxicity is endangered to fragrant-flowered garlic maggot, by at indoor test Diacloden nematode Steinernema carpocapsae SteinernemacarpocapsaeAll and blister beetle Steinernema feltiae SteinernemafeltiaeSF-SN survived and the impact of infection rate, under field condition, the method of the Diacloden of low concentration is added during application entomopathogenic nematode control fragrant-flowered garlic maggot, nematoda control harm leek can be significantly improved, shallot, onion, spring onion, the effect of the vegetable crop that garlic etc. are easily endangered by fragrant-flowered garlic maggot, nematode and the control of Diacloden to fragrant-flowered garlic maggot show obvious synergistic effect, the usage amount of remarkable reduction Diacloden and other insecticides, coordinate black haftplatte to the baiting technique of adult, effective reduction field insect population radix, prevention and control fragrant-flowered garlic maggot is made really to reach safety, non-harmful object.
Detailed Description Of The Invention:
Technical scheme of the present invention is as follows:
Improve a method for the anti-effect of entomopathogenic nematode control fragrant-flowered garlic maggot, comprise step as follows:
(1) soil that is loose and air capacity of soils is selected to carry out wholely, long-term cropping;
(2) by entomopathogenic nematode synergist thin up, be mixed with dilution, entomopathogenic nematode synergist dilution is mixed obtained synergy suspension with the entomopathogenic nematode meeting testing conditions, described entomopathogenic nematode synergist is Diacloden, in synergy suspension, nematode concentration is 500 ~ 1000IJS/mL, and Diacloden concentration is 12 ~ 15mg/L; Use synergy suspension in fragrant-flowered garlic maggot larval phase to kill fragrant-flowered garlic maggot larva, after using, carry out normal field management;
The above-mentioned testing conditions that meets should meet following requirement:
By the Diacloden dilution of 12 ~ 15mg/L and dilution be 500 ~ 1000IJS/mL nematode suspension by volume 100:1 ratio mix, place under the condition of 24 ~ 26 DEG C 24h, 48h, 72h detect, entomopathogenic nematode survival rate is more than 90%.
The present invention is preferred, for effectively reducing insect population radix, uses period at synergy suspension, and hang black haftplatte trapping adult in field, every mu is hung black haftplatte number is 60 ~ 70 pieces, and the distance between black haftplatte is 2 ~ 3 meters.
The present invention is preferred, and above-mentioned entomopathogenic nematode is nematode Steinernema carpocapsae SteinernemacarpocapsaeAll or blister beetle Steinernema feltiae SteinernemafeltiaeSF-SN.
The present invention is preferred, and described Diacloden is the Diacloden water dispersible granules of former medicine or 25%.
Above-mentioned entomopathogenic nematode product are this area conventional variety, can be bought obtain by Lv Nong bio tech ltd, Tai'an; Diacloden Shenzhen Rui Defeng means of agricultural production Co., Ltd is on sale.
Preferred according to the present invention, carry out the winter ploughing winter before whole ground in step (2) and execute, eliminate fragrant-flowered garlic maggot larva or clean field, reduce the generation radix of fragrant-flowered garlic maggot.
Preferred according to the present invention, in step (2), synergy suspension need be used while allocating, and as placed more than 8 hours, need be kept in 4-8 DEG C of temperature range, to ensure the survival of nematode.
Preferred according to the present invention, in step (2), crop is that leek, shallot, onion, spring onion, garlic etc. are easily by liliaceous vegetable crop that fragrant-flowered garlic maggot endangers.
Loose and that air capacity of soils is good soil is selected in described step (1), its object is to for entomopathogenic nematode survival, seek, infect host and set up the environment that Natural Population provides favourable, therefore those skilled in the art can according to above-mentioned purpose conveniently technology select.
Preferred according to the present invention, in described step (2) synergy suspension use in the following way: adopt the mode of irrigating of spreading manuer in holes, spray or directly water to use.
Preferred further, described spraying adopts sprayer to spray synergy suspension 2-3 time, and adjacent twice interval time is 3 days, sprays in the scope of distance rhizome portion, ground 20cm, every mu of amount of application 60 ~ 80L.
Preferred further, described directly watering is irrigated as first using nematode suspension with watering, and all infiltrates after soil spray entomopathogenic nematode synergist dilution again until water; The nematode concentration of nematode suspension is 800 ~ 900IJS/mL, and the Diacloden concentration of entomopathogenic nematode synergist dilution is 13 ~ 15mg/L.
Preferred according to the present invention, in step (2), fragrant-flowered garlic maggot larval phase, refers to the fragrant-flowered garlic maggot in below fragrant-flowered garlic maggot 3 age.
Preferred according to the present invention, in described step (2), nematode consumption is every mu of amount of application 0.6 ~ 1.2 hundred million.
Preferred according to the present invention, the bottom distance ground 0.5 ~ 1cm of black haftplatte, long limit is parallel to the ground.
Black haftplatte of the present invention is prior art, and market is buied or obtained by prior art, and Jia Duo science, industry and trade Co., Ltd is on sale; The preferred black haftplatte of the present invention obtains as follows, and adopted by plastic plate black plastic film coated, then even spread black viscose glue is made, and profile is rectangle, and length is 20 ~ 30cm, and wide is 10 ~ 15cm.
Beneficial effect
1, when the present invention utilizes nematode Steinernema carpocapsae SteinernemacarpocapsaeAll or blister beetle Steinernema feltiae SteinernemafeltiaeSF-SN to prevent and treat land for growing field crops fragrant-flowered garlic maggot, add low concentration Diacloden suspension, can significantly improve control efficiency, both coexpress are synergistic effect.5d after process, control efficiency reaches more than 92.11%, higher than the preventive effect (90.22%) of alone high concentration Diacloden, improves 52.08 ~ 86.29% than alone entomopathogenic nematode.On this basis, coordinate black colour table to the baiting technique of adult, greatly effectively reduce the insect population radix in field.30d after process, control efficiency reaches more than 98.17%, and its preventive effect is far away higher than the high concentration Diacloden used the same period and alone nematode.
2, the present invention (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m at every mu of nematode amount of application 6,000 ten thousand ~ 1.2 hundred million
2plot), in suspension, nematode concentration is 500 ~ 1000IJS/mL, nematode concentration is 30 ~ 60/head, Diacloden concentration is 12 ~ 15mg/L, 30d after process when black haftplatte suspension number is 60 ~ 70 pieces of/mu of, vermins-proof effect significantly improves, reach 98.17 ~ 99.59%, be significantly higher than the control efficiency (60.98%) of conventional alone nematode (3 ~ 400,000,000/mu) control efficiency (47.76%) and the alone Diacloden of routine (concentration is 120mg/L).Bright by long term test summary analysis, before fragrant-flowered garlic maggot larva 3 age (comprising for 3 ages), Diacloden suspension is added when using nematode SF-SN or All in field, and hang black haftplatte in the adult stage, significantly can decrease the usage amount of often kind of material, particularly considerably reduce utilization of pesticides amount, both cost-saving, reach again the object of safety, high-efficiency prevention and control fragrant-flowered garlic maggot.The present invention simultaneously can replace the desinsection insecticide varieties such as high malicious high residue, not only has ecological significance, also has important environment protection significance simultaneously.
3, biological control, chemical control and physical control combine by prevention and controls of the present invention, the method of low concentration Diacloden is added when utilizing entomopathogenic nematode to prevent and treat fragrant-flowered garlic maggot larva, the control efficiency of nematode can be significantly improved, utilize black colour table to trap and kill fragrant-flowered garlic maggot adult simultaneously, thus effectively reduce the object of insect population radix, greatly can not only improve the control efficiency to fragrant-flowered garlic maggot larva and adult, and entomopathogenic nematode and utilization of pesticides amount can be reduced, really realize fragrant-flowered garlic maggot insect non-environmental pollution control target.
Accompanying drawing explanation
Fig. 1 different insecticides is to the block diagram of nematode survival;
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further elaborated, but institute of the present invention protection domain is not limited thereto.
Laboratory test measures
1, nematode and insecticide compatibility measure
40% chlorpyrifos ec is purchased from Zhejiang Yongnong Bioscience Co., Ltd.; 4.5% r is purchased from the large bright agrochemical Co., Ltd of Jiangmen city; 25% Diacloden water dispersible granules is purchased from Rui Defeng means of agricultural production Co., Ltd of Shenzhen.Nematode Steinernema carpocapsae SteinernemacarpocapsaeNC116, nematode Steinernema carpocapsae SteinernemacarpocapsaeAll, S.longicaudum SteinernemalongicaudumX-7, blister beetle Steinernema feltiae SteinernemafeltiaeSF-SN, Heterorhabditis bacteriophora-NJ HeterorhabditisbacteriophoraH06, India heterorhabditis indica HeterorhabditisindicaLN2.Above-mentioned entomopathogenic nematode is this area and commonly uses entomopathogenic nematode, and Lv Nong bio tech ltd, Tai'an is on sale.
Respectively by the Diacloden (120mg/L) of the highest for field working concentration, chlopyrifos (200mg/L) and beta-cypermethrin (25mg/L) dilution and dilution for 500IJS/mL nematode suspension by volume 100:1 ratio mix, 3 repetitions are established in each process, and the clear water nematode suspension being 500IJS/mL with nematode concentration compares.Under the condition of (25 ± 1) DEG C, place 24h, under microscope, the amount of survival of nematode in each process casual inspection 1mL mixed liquor, calculates survival rate.If insecticide, not more than 10% then continues to measure this insecticide to the impact of nematode infection rate to the lethal effect of nematode or sub-lethal effect.Judge that entomopathogenic nematode is to the reaction normal of medicament with reference to Wei Hong justice: (1) is dead: body rigidity or curling, and inertia is reactionless to acupuncture; (2) sub-lethal: health is curling, because the effect of medicament paralysis causes spastic or convulsive activity, reactionless or reaction is very slow to acupuncture; (3) do not affect by medicament: the same with contrast nematode, body movement is unfolded or transfixion, static nematode is swift in response after probe touching.
Result of the test (Fig. 1) shows: different insecticides all has lethality rate in various degree to nematode, but has not exceeded the lethal upper limit of 10%.Insecticide is not remarkable with the mortality difference of the nematode that nematode mixed liquor nemic death rate contrasts with clear water.Show three kinds of insecticide chlorpyrifos, beta-cypermethrin, the survival of Diacloden on nematode do not affect substantially.
Insecticide indoor action effect to fragrant-flowered garlic maggot mixed with nematode measures
Respectively by entomopathogenic nematode NC116, H06, SF-SN, All, H06, LN of different insecticides Diacloden (12mg/L), chlopyrifos (20mg/L) and beta-cypermethrin (2.5mg/L), 100/worm concentration
2and each insecticide and nematode mixed liquor process and clear water contrast.In the culture dish of diameter 9cm, put two Medium speed filter papers, drip each treatment fluid 1.5mL respectively, put into 30 3 age fragrant-flowered garlic maggot and appropriate leek stem section, often 4 repetitions are established in process, do blank with distilled water process.Be placed in the incubator of temperature (22 ± 1) DEG C, humidity (60 ± 10) %.Because of fragrant-flowered garlic maggot happiness dark surrounds, therefore in culture dish peripheral closure black cloth, avoid fragrant-flowered garlic maggot to creep outside ware.After process 3d, touch polypide with little writing brush point, motionless person is designated as death, calculates corrected mortality.
Action effect adopts Zhang Zhongrun method to calculate, by χ
2independence test judges that nematode and insecticide are to the synergy type of fragrant-flowered garlic maggot.Work as χ
2during < 3.84, show that two kinds of desinsection factors are mixed shows as summation action, work as χ
2>=3.84 and M
nI< M
etime, show that two kinds of desinsection factors are mixed shows as antagonism, work as χ
2>=3.84 and M
nI> M
etime, show that two kinds of desinsection factors are mixed shows as synergistic effect.M=[M
n+ M
i(1-M
n)]; M
e=M*N; χ
2=(M
nI-M
e)
2/ M
e, wherein, M, M
erepresent that nematode and Mixture Use of Insecticides try the expectation lethality rate of insect and lethal number to supplying respectively; M
nand M
ibe respectively nematode and medicament and process separately corrected mortality for examination insect; M
nIfor nematode and Mixture Use of Insecticides are to supplying the actual correction death toll trying insect; N is for supplying examination insect populations.
Table 1 nematode and Mixture Use of Insecticides are to the interaction of fragrant-flowered garlic maggot
Note: D
i, D
nand D
nIrepresent insecticide respectively, corrected mortality that nematode, insecticide+nematode use lower fragrant-flowered garlic maggot 3 instar larvae with.
Indoor measurement result (shown in table 1) shows, entomopathogenic nematode and insecticide coupling obtain nematode killer suspension, insecticidal effect (D
nI) (D more alone than insecticide comparable sodium
i), the alone (D of nematode
n) all significantly improve.Wherein obvious synergistic effect is shown to the control of fragrant-flowered garlic maggot, wherein LN in nematode and Diacloden suspension
2nematode, SF-SN nematode and All nematode are when adding Diacloden, and its vermins-proof effect reaches more than 93%.
Diacloden field action effect to fragrant-flowered garlic maggot mixed with nematode measures
Field trial
Fragrant-flowered garlic maggot is the important pests of harm leek, the liliaceous vegetables such as main harm leek, shallot, onion, spring onion, garlic.Test is at Shandong Province's fruit tree ten thousand lucky mountain Experimental Bases, and soil property is loose and that air capacity of soils is good sandy loam.Leek kind is that the only son and heir is red, the quantity of Pre-trial Investigation leek ground fragrant-flowered garlic maggot larva, and select the basically identical plot of fragrant-flowered garlic maggot quantity to test, each plot area is 40m
2, between different disposal, isolated area is set, repeats 4 times, randomized arrangement.Nematode and nematode Diacloden suspension is poured near leek plant.Nematode usage amount is 60kg nematode suspension/mu." Z " shape sampling method is adopted to investigate 5 points in every community respectively at 3d and 7d after medicine, often some investigation 5 strains.Investigate respectively and record every strain fragrant-flowered garlic maggot survival number, calculating vermins-proof effect.The Diacloden of above-mentioned screening is utilized to test.In suspension, nematode concentration is 500 ~ 1000IJS/mL, and every mu of nematode amount of application 400,000,000, is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot, nematode concentration is 200/head, and Diacloden concentration is 12mg/L, and actual conditions step is in table 2.
The method of process tested by table 2
Table 3 Diacloden compares entomopathogenic nematode control fragrant-flowered garlic maggot field efficacy
As can be seen from Table 3, after adding Diacloden in nematode suspension, the Be very effective of its control fragrant-flowered garlic maggot is better than alone nematode effect.Wherein show better LN in laboratory test (table 1)
2nematode+Diacloden, then do not show good vermins-proof effect, this and nematode LN
2nematode field adaptive capacity difference is relevant.Wherein control efficiency is it is preferred that nematode All strain+Diacloden and SF-SN strain+Diacloden, and vermins-proof effect reaches more than 95%.
Best synergistic formula laboratory experiment
Establish Diacloden (5 ~ 20mg/L content range) respectively and be respectively entomopathogenic nematode SF-SN, All of 30,60,100/worm concentration and the Diacloden of each content range and nematode mixed liquor process and clear water contrast.In the culture dish of diameter 9cm, put two Medium speed filter papers, drip each treatment fluid 1.5mL respectively, put into 30 3 age fragrant-flowered garlic maggot and appropriate leek stem section, often 4 repetitions are established in process, do blank with distilled water process.Be placed in the incubator of temperature (22 ± 1) DEG C, humidity (60 ± 10) %.Because of fragrant-flowered garlic maggot happiness dark surrounds, therefore in culture dish peripheral closure black cloth, avoid fragrant-flowered garlic maggot to creep outside ware.The little writing brush point of process 3d touches polypide, and motionless person is designated as death, calculates corrected mortality.
Table 4 synergistic formula laboratory experiment result
Indoor measurement result (table 4) shows, adds Diacloden in entomopathogenic nematode, insecticidal effect (D
nI) (D more alone than Diacloden comparable sodium
i), the alone (D of nematode
n) all significantly improve.In use Diacloden (in 12 ~ 20mg/L concentration range), nematode SF-SN and All (30 ~ 100 in concentration range) reaches more than 90% to the control efficiency of fragrant-flowered garlic maggot, shows obvious synergistic effect.In nematode All, SF-SN of working concentration 30 ~ 60/worm, wherein add Diacloden (12 ~ 15mg/L) and use 20mg/L Diacloden and the control efficiency difference of 100/worm nematode to fragrant-flowered garlic maggot not obvious, obviously can reduce the usage amount of nematode.
Synergistic formula field trial is verified
Test is at Shandong Province's fruit tree ten thousand lucky mountain Experimental Bases, and soil property is loose and that air capacity of soils is good sandy loam.Leek kind is that the only son and heir is red, the quantity of Pre-trial Investigation leek ground fragrant-flowered garlic maggot larva, and select the basically identical plot of fragrant-flowered garlic maggot quantity to test, each plot area is 40m
2, between different disposal, isolated area is set, repeats 4 times, randomized arrangement.Nematode and nematode Diacloden suspension is poured near leek plant.Nematode usage amount is 60kg nematode suspension/mu." Z " shape sampling method is adopted to investigate 5 points in every community, often some investigation 5 strains.Investigate respectively and record every strain fragrant-flowered garlic maggot survival number, calculating vermins-proof effect.Diacloden 12 ~ 15mg/L concentration of above-mentioned screening is utilized to test.In suspension, nematode concentration is 500 ~ 1000IJS/mL, every mu of nematode amount of application 6,000 ten thousand ~ 1.2 hundred million, is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot, nematode concentration is 30 ~ 60/head.Black colour table suspension height is colour table lower edge overhead 1cm place.Respectively at using rear 5d, 30d investigation of process to first brood of larvae and second generation larva control efficiency.
Embodiment 1
Improve a method for the anti-effect of entomopathogenic nematode control fragrant-flowered garlic maggot, step is as follows:
(1) the winter ploughing winter executes, and eliminates fragrant-flowered garlic maggot larva or clean field, reduces the generation radix of fragrant-flowered garlic maggot, and select soil that is loose and air capacity of soils to carry out wholely, plantation leek, leek kind is that the only son and heir is red;
(2) by entomopathogenic nematode synergist thin up, be mixed with dilution, entomopathogenic nematode synergist dilution is mixed obtained synergy suspension with the nematode Steinernema carpocapsae SteinernemacarpocapsaeAll meeting testing conditions, described entomopathogenic nematode synergist is Diacloden, in synergy suspension, nematode concentration is 800IJS/mL, and Diacloden concentration is 12mg/L; Before fragrant-flowered garlic maggot Larvae occurrence time 3 age (comprising for 3 ages), pour into synergy suspension and kill fragrant-flowered garlic maggot larva near leek plant, nematode consumption is 6,000 ten thousand/mu and (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot), use period at synergy suspension, hang black haftplatte trapping adult in field, every mu is hung black haftplatte number is 70 pieces, and the distance between black haftplatte is 2.5 meters.Normal field management is carried out after using;
Above-mentionedly meet the requirement that testing conditions should meet following a:
A: by the Diacloden dilution of 12mg/L and dilution for 800IJS/mL nematode suspension by volume 100:1 ratio mix, place under the condition of 24 ~ 26 DEG C 24h, 48h, 72h detection, entomopathogenic nematode survival rate is more than 90%.
Embodiment 2
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 1, difference is,
In synergy suspension, nematode concentration is 800IJS/mL, and Diacloden concentration is 15mg/L; Nematode consumption is 1.2 hundred million/mu and (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot), use period at synergy suspension, hang black haftplatte trapping adult in field, every mu is hung black haftplatte number is 60 pieces.
Embodiment 3
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 1, difference is,
Entomopathogenic nematode is cyanines Steinernema feltiae SteinernemafeltiaeSF-SN, and in synergy suspension, nematode concentration is 800IJS/mL, and Diacloden concentration is 12mg/L; Nematode consumption is 6,000 ten thousand/mu and (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot), use period at synergy suspension, hang black haftplatte trapping adult in field, every mu is hung black haftplatte number is 70 pieces.
Embodiment 4
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 1, difference is,
Entomopathogenic nematode is cyanines Steinernema feltiae SteinernemafeltiaeSF-SN, and in synergy suspension, nematode concentration is 800IJS/mL, and Diacloden concentration is 15mg/L; Nematode consumption is 1.2 hundred million/mu and (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot), use period at synergy suspension, hang black haftplatte trapping adult in field, every mu is hung black haftplatte number is 60 pieces.
Comparative example 1
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 2, difference is, field does not hang colour table, carries out normal field management after using.
Comparative example 2
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 4, difference is, field does not hang colour table, carries out normal field management after using.
Comparative example 3
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 1, difference is, pour near leek plant and add concentration 20mg/L Diacloden and nematode Steinernema carpocapsae SteinernemacarpocapsaeAll suspension, entomopathogenic nematode consumption is 200,000,000/mu and (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot), every mu is hung black haftplatte number is 60 pieces, carries out normal field management after using.
Comparative example 4
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 1, difference is, pour near leek plant and add concentration 20mg/L Diacloden and cyanines Steinernema feltiae SteinernemafeltiaeSF-SN suspension, entomopathogenic nematode consumption is 200,000,000/mu and (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot), every mu is hung black haftplatte number is 60 pieces, carries out normal field management after using.
Comparative example 5
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 1, difference is, pour near leek plant and add concentration 10mg/L Diacloden and nematode Steinernema carpocapsae SteinernemacarpocapsaeAll suspension, entomopathogenic nematode consumption is 200,000,000/mu and (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot), every mu is hung black haftplatte number is 60 pieces, carries out normal field management after using.
Comparative example 6
With the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot of embodiment 1, difference is, pour near leek plant and add concentration 10mg/L Diacloden and cyanines Steinernema feltiae SteinernemafeltiaeSF-SN suspension, entomopathogenic nematode consumption is 200,000,000/mu and (is applicable to fragrant-flowered garlic maggot insect density 300 ~ 3000/m
2plot), every mu is hung black haftplatte number is 60 pieces, carries out normal field management after using.
Embodiment 1-4 and comparative example 1-6 implementation condition are shown in Table 5.
The method of process tested by table 5
The field test results of embodiment 1-4 and comparative example 1-6 to control fragrant-flowered garlic maggot is shown in Table 6.
Table 6 Diacloden is to the field trial of nematoda control fragrant-flowered garlic maggot
As can be seen from Table 6,5d after process, the vermins-proof effect of embodiment 1, embodiment 2, embodiment 3, embodiment 4, comparative example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5 and comparative example 6 pairs of fragrant-flowered garlic maggots is higher than alone nematode SF-SN, All, the same with use high concentration Diacloden, control efficiency all reaches more than 90%.Add concentration be 12,15 and 20mg/L Diacloden nematode suspension in the control efficiency of fragrant-flowered garlic maggot higher than adding 10mg/L's, control efficiency improves 22.68 ~ 28.44%, but add 12 and 15mg/L Diacloden with to add 20mg/L Diacloden control efficiency difference little.Add the Diacloden of 12 ~ 15mg/L in embodiment 1, embodiment 2, embodiment 3 and embodiment 4, its vermins-proof effect improves 52.08 ~ 86.29% than alone nematode.Process 30d, owing to having hung blackboard, improve the trapping effect to first generation fragrant-flowered garlic maggot adult, the vermins-proof effect of embodiment 1, embodiment 2, embodiment 3 and embodiment 4 reaches more than 94.11%, be significantly higher than alone nematode group, alone Diacloden group and the Diacloden nematode group adding 10mg/L.Summary analysis is bright before fragrant-flowered garlic maggot larva 3 age (comprising for 3 ages), nematode SF-SN or All is used in field, during control fragrant-flowered garlic maggot, the Diacloden suspension adding 12 ~ 15mg/L can significantly improve control efficiency, and hanging black colour table trapping adult, vermins-proof effect reaches 98.17 ~ 99.59%.
Claims (10)
1. improve a method for the anti-effect of entomopathogenic nematode control fragrant-flowered garlic maggot, comprise step as follows:
(1) soil that is loose and air capacity of soils is selected to carry out wholely, long-term cropping;
(2) by entomopathogenic nematode synergist thin up, be mixed with dilution, entomopathogenic nematode synergist dilution is mixed obtained synergy suspension with the entomopathogenic nematode meeting testing conditions, described entomopathogenic nematode synergist is Diacloden, in synergy suspension, nematode concentration is 500 ~ 1000IJS/mL, and Diacloden concentration is 12 ~ 15mg/L; Use synergy suspension in fragrant-flowered garlic maggot larval phase to kill fragrant-flowered garlic maggot larva, after using, carry out normal field management;
The above-mentioned testing conditions that meets should meet following requirement:
By the Diacloden dilution of 12 ~ 15mg/L and dilution be 500 ~ 1000IJS/mL nematode suspension by volume 100:1 ratio mix, place under the condition of 24 ~ 26 DEG C 24h, 48h, 72h detect, entomopathogenic nematode survival rate is more than 90%.
2. the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot according to claim 1, it is characterized in that, for effectively reducing insect population radix, period is used at synergy suspension, black haftplatte trapping adult is hung in field, every mu is hung black haftplatte number is 60 ~ 70 pieces, and the distance between black haftplatte is 2 ~ 3 meters.
3. the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot according to claim 1, it is characterized in that, above-mentioned entomopathogenic nematode is nematode Steinernema carpocapsae SteinernemacarpocapsaeAll or blister beetle Steinernema feltiae SteinernemafeltiaeSF-SN; In synergy suspension, nematode concentration is 800 ~ 900IJS/mL.
4. the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot according to claim 1, it is characterized in that, described Diacloden is the Diacloden water dispersible granules of former medicine or 25%.
5. the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot according to claim 1, is characterized in that, carry out the winter ploughing winter and execute in step (2) before whole ground, eliminate fragrant-flowered garlic maggot larva or clean field, reduce the generation radix of fragrant-flowered garlic maggot.
6. the method for the anti-effect of raising entomopathogenic nematode according to claim 1 control fragrant-flowered garlic maggot, is characterized in that, in step (2), crop is that leek, shallot, onion, spring onion, garlic etc. are easily by liliaceous vegetable crop that fragrant-flowered garlic maggot endangers.
7. the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot according to claim 1, it is characterized in that, in described step (2) synergy suspension use in the following way: adopt the mode of irrigating of spreading manuer in holes, spray or directly water to use.
8. the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot according to claim 7, it is characterized in that, described spraying adopts sprayer to spray synergy suspension 2-3 time, adjacent twice interval time is 3 days, spray in the scope of distance rhizome portion, ground 20cm, every mu of amount of application 60 ~ 80L; Described directly watering is irrigated as first using nematode suspension with watering, and all infiltrates after soil spray entomopathogenic nematode synergist dilution again until water; The nematode concentration of nematode suspension is 800 ~ 900IJS/mL, and the Diacloden concentration of entomopathogenic nematode synergist dilution is 13 ~ 15mg/L.
9. the method for the anti-effect of raising entomopathogenic nematode according to claim 1 control fragrant-flowered garlic maggot, is characterized in that, in step (2), fragrant-flowered garlic maggot larval phase refers to the fragrant-flowered garlic maggot in below fragrant-flowered garlic maggot 3 age, and nematode consumption is every mu of amount of application 0.6 ~ 1.2 hundred million.
10. the method for the anti-effect of raising entomopathogenic nematode control fragrant-flowered garlic maggot according to claim 2, is characterized in that, the bottom distance ground 0.5 ~ 1cm of black haftplatte, long limit is parallel to the ground.
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| CN115669433A (en) * | 2021-07-21 | 2023-02-03 | 南开大学 | Method for preventing and controlling dipteran pests |
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