CN106665527A - Entomopathogenic nematodes population density detection device and entomopathogenic nematodes population density detection method - Google Patents
Entomopathogenic nematodes population density detection device and entomopathogenic nematodes population density detection method Download PDFInfo
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- CN106665527A CN106665527A CN201611179421.0A CN201611179421A CN106665527A CN 106665527 A CN106665527 A CN 106665527A CN 201611179421 A CN201611179421 A CN 201611179421A CN 106665527 A CN106665527 A CN 106665527A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/10—Catching insects by using Traps
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/023—Attracting insects by the simulation of a living being, i.e. emission of carbon dioxide, heat, sound waves or vibrations
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- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Insects & Arthropods (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to an entomopathogenic nematodes population density detection device and an entomopathogenic nematodes population density detection method. The entomopathogenic nematodes population density detection device comprises a acquisition tube and a capture tube. The acquisition tube comprises an inner acquisition tube body and an outer acquisition tube body, the bottom end of the inner acquisition tube body is in threaded connection with a trapping net cover, the capture tube is positioned at the bottom end of the outer acquisition tube body and is in threaded connection with the outer acquisition tube body, the trapping net cover is positioned in the outer acquisition tube body, the sidewall of the capture tube is provided with 2-3 trapping holes in a surrounding manner, the capture tube is filled with clear water, a non-woven fabric for sealing the trapping holes is arranged on the inner wall of the capture tube, and the lower end of the non-woven fabric extends into the clear water. During detection, Chinese chive maggots and Chinese chives roots are added into the net cover to serve as trapping substances and release volatile substances and carbon dioxide to trap entomopathogenic nematodes. The entomopathogenic nematodes population density detection device and the entomopathogenic nematodes population density detection method have the advantages that absolute error is 0.429 after detection and is 6.75 times of a detection quantity obtained by a device numbered CN101849534A; the method and the device can be used for field acquisition of the entomopathogenic nematodes and tracking and determination of population density of the entomopathogenic nematodes in field soil.
Description
Technical field
The present invention relates to a kind of entomopathogenic nematode population density detection means and detection method, belong to biological Pesticidal technology
Field.
Background technology
Entomopathogenic nematode (entomopathogenic nematodes, EPNs) is the line of the special parasitic insect of a class
Worm, enters in host's body by way of 3 age infective stage larvas are invaded to the naturally open or coria of insect body, and discharges
The symbiotic bacteria of carrying so as to breed in insect body, produce toxin, ultimately result in insect dead.Because EPNs has host's model
Enclose extensively, actively find host, to non-target organism safety, environment is had no side effect, it is can be mixed with many pesticide, fertilizer etc. excellent
Point, is widely used to various agricultural subterranean pest-insects such as control of grubs, cutworm, small heart-eating peach worm, Anoplophorae seu Aprionae.
During using entomopathogenic nematode pest control, how effectively to obtain from soil and be suitable for local life
Nematicide strain under the conditions of state is successfully the key of pest control.Such work mainly adopts greater wax moth (Galleria at present
Mellonella) the method that live body is trapped, concretely comprises the following steps after field fetches earth, soil sample is divided in plastic casing, one is added
The greater wax moth larva of fixed number amount is trapped.This method needs to collect through greater wax moth infection, dissection and nematicide and detected
Journey, it is bothersome laborious, and the method needs to take larger test site, takes a significant amount of time and carrys out checking experiment result.Therefore greatly
Limit nematicide field collect.
In addition, after Field information nematicide, needing to monitor survival condition of the nematicide in soil once to apply on determining
Necessity, application practice and consumption.The method that this process is still trapped using greater wax moth live body at present, the limitation except more than
Property outside, field sampling certainly will destroy field crops.
Additionally, entomopathogenic nematode is can not only be in pest body as one of a kind of insect biocontrol factor, its advantage
Interior amount reproduction, and can be survived in soil for a long time, with the ability for adjusting pest population quantity, Sustainable Control insect.
Therefore, how to obtain field in different times is then effectively to play nematicide to adjust insect using the population density of certain nematicide strain
Population quantity, the another key of ability of Sustainable Control insect.Such work mainly adopts Molecular Detection means at present, i.e., by reality
When quantitative fluorescent PCR method.The method is loaded down with trivial details, and expense is high, is unfavorable for large-area popularization and application.
Such as：Chinese patent literature CN101849534 A discloses a kind of entomopathogenic nematode and separates and detection means, can use
In soil, entomopathogenic nematode is separated and population density measurement.Although the device does not destroy the normal growth of crop, the dress
Put and trapped using greater wax moth, consumption time is long, and nematicide is separated and carried out with device for detecting density respectively, is wasted time and energy,
There is significant limitation in the application, while also there is the nematicide for trapping in actual applications easily along lacking that Cotton Gossypii is fled from
Fall into.
The content of the invention
For the deficiencies in the prior art, the present invention provides a kind of entomopathogenic nematode population density detection means and detection side
Method.
Summary of the invention：
The present invention for nematicide collection and statistics population quantity when, need gather soil sample, investigation greater wax moth infection conditions,
Dissect polypide, the problem wasted time and energy caused by the institute such as destruction, there is provided a kind of entomopathogenic nematode population density detection is filled
Put, the apparatus structure is simple, colour paper is high, and nematicide is not easy to flee from；Nematicide is lured by the device to test different material
The evaluation of collection ability；The fragrant-flowered garlic maggot quantity of fragrant-flowered garlic maggot+Radix Folium Allii tuberosi combination is evaluated to trapping the impact of nematicide；Specify fragrant-flowered garlic maggot+Radix Folium Allii tuberosi group
Conjunction traps the Best Times of nematicide；And detector places the optimization of spacing；That what is finally set up under different nematicide concentration traps line
The standard curve of worm.The device and method can be applied and eat Zuo Wu ﹑ oil crop, vegetable crop etc. and forestry in agricultural such as Liang
Detect etc. the line insect population under various modes.Line insect population detection can also be carried out used in bare place plot simultaneously.
Technical scheme is as follows：
A kind of entomopathogenic nematode population density detection means, the device include the collection tube of two-layer tubular structure and seizure
Pipe, collection tube include gathering inner tube and collection outer tube two-layer, and collection inner tube and collection outer tube two ends are open settings, collection inner tube
Bottom be threaded with and trap guard, catch pipe positioned at the bottom of collection outer tube and be connected with collection external pipe thread, trap net
Cover in collection outer tube catches pipe for upper end open, the tubular structure of lower end closed, is provided with 2- around the side wall for catching pipe
3 trap hole, be filled with clear water catching in pipe, and the non-woven fabrics that sealing traps hole are provided with the inwall for catching pipe, and
The lower end of non-woven fabrics is extended in clear water.
Currently preferred, it is 2~10mm to gather the spacing between inner tube and collection outer tube.Tested by numerous experiments
Card, the spacing gathered between inner tube and collection outer tube are optimal to gathering effect, and spacing is excessive, and it is few to trap the nematode population for obtaining,
Spacing is excessive, inconvenient operation and nematode population is few.
The present invention is highly preferred, and it is 4~5mm to gather the spacing between inner tube and collection outer tube.
Currently preferred, the top of collection tube is provided with sealing-plug and will gather the seal of tube simultaneously by outside collection inner tube and collection
Pipe links together.
Currently preferred, the height for trapping guard is 15~20mm, and the internal diameter and collection inner tube for trapping guard matches.
Currently preferred, the height for catching pipe is 35~45mm, and the internal diameter and collection outer tube for catching pipe matches.
Currently preferred, the bottom distance for trapping guard traps 1~4mm of hole lower end, highly preferred, traps guard
Bottom distance traps hole lower end 2mm.The distance both ensure that optimal gathering effect, and effectively prevent the nematicide for trapping from escaping
From.
Currently preferred, it is 4~7mm that the lower end of non-woven fabrics extends to the length of clear water, highly preferred, non-woven fabrics
It is 5mm that lower end extends to the length of clear water.The length of extension can make nematicide carry out clear water more easily by moisture in non-woven fabrics
In, and be difficult to again flee from soil into nematicide after clear water.
It is currently preferred, the 5~60cm of height of collection tube.The height of collection tube is met in collection different depth soil
Nematicide.
According to practical application, the 5~25cm of height of collection tube.
It is currently preferred, hole is trapped for two, two to trap the spacing between hole be 0.5~2mm.
The method that entomopathogenic nematode population density is detected using above-mentioned entomopathogenic nematode population density detection means, bag
Include step as follows：
(1) select to need the plot of detection, to be detected using line insect population device for detecting density；
(2) collection tube of different model is installed according to the nematicide in detection different depth soil, by collection tube and seizure pipe
Separate, then will trap material and load in guard, and load in pipe clear water to trapping at hole catch, then will fill catching for clear water
Catch pipe and trap material guard and be installed together with being equipped with；Described traps the combination that material is fragrant-flowered garlic maggot and Radix Folium Allii tuberosi, fragrant-flowered garlic maggot and fragrant-flowered garlic
The head number of dish root with radical ratio is：(5~25)：(3~6), the length of Radix Folium Allii tuberosi is 0.5~2cm；
(3) hole is made a call on soil to be detected, then by the seizure pipe being installed together in step (2), collection tube
Entirety is put in hole, and be intervally arranged detection means, it is ensured that trap the nematicide of maximum quantity, and wherein collection tube catches pipe and exists upper
Under；
(4) after placing 40~50h, detection means is fetched from hole, then lays down seizure pipe, and the water caught in pipe is inhaled
After going out, the number of nematicide in water is examined under a microscope；
(5) according to the nematicide number observed in step (4), calculate the population density for obtaining nematicide in soil.
According to currently preferred, in step (1), the humidity of soil to be measured is 10%-18%, and temperature is at 15~35 DEG C.For
Ensure that the nematicide diffusivity of nematicide in soil under this environmental condition is basically identical.Humidity, temperature it is too low or it is too high all
Nematicide diffusivity is influenced whether, and then has influence on the quantity for trapping nematicide.
Currently preferred, fragrant-flowered garlic maggot with the head number of Radix Folium Allii tuberosi with radical ratio is：(15~25)：(4~6), the length of Radix Folium Allii tuberosi
Spend for 1cm.
Highly preferred, fragrant-flowered garlic maggot with the head number of Radix Folium Allii tuberosi with radical ratio is：20：4.
Currently preferred, the spacing that step (3) detection means is intervally arranged is 10~14cm, and each process is repeated 4 times
Trapped.
Currently preferred, step (4) detection means standing time in hole is 48h.
Currently preferred, the fragrant-flowered garlic maggot is the fragrant-flowered garlic maggot living in 3 ages.
The collection tube of two-layer tubular structure of the present invention traps nematicide more preferably using the abnormal smells from the patient of fragrant-flowered garlic maggot+Radix Folium Allii tuberosi, while greatly
The big gathering effect for improving nematicide, and hinder.By the greater wax moth children in guard
Worm is improved to the combination of fragrant-flowered garlic maggot+Radix Folium Allii tuberosi, and this design is the interaction according to nematicide, fragrant-flowered garlic maggot and Radix Folium Allii tuberosi, using Folium Allii tuberosi
Root is caused harm by fragrant-flowered garlic maggot.
Beneficial effect
1st, the present invention improves detection means, obtains a kind of entomopathogenic nematode population density detection means and its user
Method, the apparatus and method design device using double layered tubular, using fragrant-flowered garlic maggot+Radix Folium Allii tuberosi release volatile material and carbon dioxide come
Nematicide is trapped, it is 2 elongated holes to be designed as around guard surrounding that improvement traps hole, improve nonwoven strip sealing peace on collection tubular construction
During dress position makes its lower end immersion clear water, make nematicide clear water be carried out more easily by moisture in non-woven fabrics, and prevent from entering clear water
Nematicide afterwards is fled from soil again, and the height of collection tube can be designed as different model on this basis, meets collection
Nematicide in different depth soil, these improvement improve and trap nematicide effect, and its absolute error minimum 0.429 is
6.75 times of CN101849534A disclosed devices institute amount detection, show best Detection results.
2nd, apparatus of the present invention and method can replace the method that routine greater wax moth live body traps nematicide, solve
The low problem of gathering effect in CN101849534A devices, improves nematicide Detection results, is capable of achieving in real-time monitor on field soil
Nematicide is in quantity and survival condition, and field sampling will not destroy field crops, with preferable application prospect.
Description of the drawings
Fig. 1 is the structural representation of entomopathogenic nematode population density detection means collection tube of the present invention；
Fig. 2 is that entomopathogenic nematode population density detection means of the present invention traps guard and catches the structural representation of pipe；
Fig. 3 is entomopathogenic nematode population density detection means overall structure diagram of the present invention；
Wherein, 1, collection tube；2nd, catch pipe；3rd, sealing-plug；4th, gather inner tube；5th, gather outer tube；6th, tap；7th, trap
Guard；8th, catch tube side wall；9th, trap hole；10th, non-woven fabrics 10；11st, clear water.
Block diagram of Fig. 4 different materials to entomopathogenic nematode gathering effect；
Fig. 5 varying numbers fragrant-flowered garlic maggot+block diagram of the Radix Folium Allii tuberosi combination to entomopathogenic nematode gathering effect；
Fig. 6 difference sample times the curve chart to entomopathogenic nematode gathering effect；
Fig. 7 detectors place spacing to the curve chart to entomopathogenic nematode gathering effect；
Fig. 8 fragrant-flowered garlic maggot+Radix Folium Allii tuberosi combination traps trend canonical plotting to entomopathogenic nematode.
Specific embodiment
Technical scheme is further elaborated with reference to embodiment, but institute's protection domain of the present invention is not limited to
This.
Embodiment 1
A kind of entomopathogenic nematode population density detection means, as shown in Figure 1-Figure 3, the device includes two-layer tubulose to structure
The collection tube 1 and seizure pipe 2 of structure, collection tube 1 include gathering inner tube 4 and collection 5 two-layer of outer tube, and collection inner tube 4 and collection are outer
5 two ends of pipe are open setting, and the bottom for gathering inner tube 4 is threaded with and traps guard 7, catches the bottom that pipe 2 is located at collection outer tube 5
End is simultaneously threadedly coupled with collection outer tube 5, is trapped guard 7 and is located in collection outer tube 5, and seizure pipe 2 is upper end open, lower end closed
Tubular structure, is provided with 2 around the side wall for catching pipe 2 and traps hole 9, and two spacing trapped between hole are 1mm, trap hole 9
Width is 10mm, be filled with clear water 11 catching, the non-woven fabrics that sealing traps hole are provided with the inwall for catching pipe 2 in pipe 2,
And the lower end of non-woven fabrics 10 is extended in clear water 11.
Spacing between collection inner tube 4 and collection outer tube 5 is 4mm, and the top of collection tube 1 is provided with sealing-plug 3, will gather
Collection inner tube and collection outer tube are simultaneously linked together by the seal of tube.
The height for trapping guard 7 is 18mm, and the internal diameter and collection inner tube 4 for trapping guard 7 matches.Catch the height of pipe 2
For 40mm, the internal diameter and collection outer tube 5 of seizure pipe 2 match.
The bottom distance for trapping guard 7 traps 9 lower end 2mm of hole.The distance both ensure that optimal gathering effect, and effectively
The nematicide for trapping that prevents flee from.It is 5mm that the lower end of non-woven fabrics extends to the length of clear water.The length of extension can make nematicide more
Carry out in clear water easily by moisture in non-woven fabrics, and be difficult to again flee from soil into nematicide after clear water.The height of collection tube 1
5~60cm of degree.The height of collection tube meets the nematicide in collection different depth soil.
Embodiment 2
With a kind of entomopathogenic nematode population density detection means described in embodiment 1, difference is,
Spacing between collection inner tube 4 and collection outer tube 5 is 6mm.
Embodiment 3
With a kind of entomopathogenic nematode population density detection means described in embodiment 1, difference is,
Spacing between collection inner tube 4 and collection outer tube 5 is 2mm.
Embodiment 4
With a kind of entomopathogenic nematode population density detection means described in embodiment 1, difference is,
The bottom distance for trapping guard 7 traps 9 lower end 1mm of hole.
Embodiment 5
With a kind of entomopathogenic nematode population density detection means described in embodiment 1, difference is,
The bottom distance for trapping guard 7 traps 9 lower end 4mm of hole.
Laboratory test is determined
1st, comparison of the different materials to entomopathogenic nematode gathering effect
From the limited public affairs of the green agriculture biotechnology in nematode Steinernema carpocapsae Steinernema carpocapsae All Tai'ans
Department is on sale.
5kg sterilized soils are loaded in round plastic basin, sterilized water and nematicide suspension is added, is maintained at soil moisture
10% or so.Infective juvenile (IJS) is by 450,000 IJS/m2Inoculation nematicide, entomopathogenic nematode kind group detector is inserted perpendicularly into
In plastic casing.Test is separately added into the Radix Folium Allii tuberosi after 2 greater wax moths, 20 fragrant-flowered garlic maggots, mechanical damage, 20 fragrant-flowered garlic maggots+4 in guard
Root 1cm Radix Folium Allii tuberosis and blank.The nematode population trapped in 48h investigation kind group detector after inoculation nematicide.Collect
Nematicide suspension in detector is counted under the microscope, and each process is repeated 4 times, and detection means spacing is 10cm.
Result of the test (Fig. 4) shows：5 are processed to entomopathogenic nematode gathering effect significant difference, wherein in guard
Add the nematode population that the combination of fragrant-flowered garlic maggot+Radix Folium Allii tuberosi is trapped to be significantly higher than other process, be greater wax moth trap 3.5 times of quantity with
On, show that fragrant-flowered garlic maggot+Radix Folium Allii tuberosi combination is more beneficial for trapping for nematicide.
2nd, varying number fragrant-flowered garlic maggot+comparison of the Radix Folium Allii tuberosi combination to entomopathogenic nematode gathering effect；
Test adds the quantity of fragrant-flowered garlic maggot to be respectively 5,10,20,40,80 in guard, a number of being separately added into
Radix Folium Allii tuberosi, other test requirements documents are with test 1.
Result of the test (Fig. 5) shows：5 process different to entomopathogenic nematode gathering effect, wherein in guard respectively
The nematode population that+4 1cm Radix Folium Allii tuberosis combinations of 20,40 and 80 fragrant-flowered garlic maggots are trapped is added to be significantly higher than addition 5 and 10 fragrant-flowered garlic
The process of maggot, and difference is not notable between adding 20,40 and 80 fragrant-flowered garlic maggots+Radix Folium Allii tuberosi to combine.Therefore 20 fragrant-flowered garlic maggot+fragrant-flowered garlic are adopted
The combination of dish root is further tested.
3rd, different impacts of the sample time to entomopathogenic nematode gathering effect
Test in guard add+4 1cm Radix Folium Allii tuberosis of 20 fragrant-flowered garlic maggots, respectively inoculation nematicide after 12,24,36,48,60,
72nd, the nematode population trapped in 84 and 96h investigation kind group detectors, other test requirements documents are with test 1.
Result of the test (Fig. 6) shows：Different sample times are different to entomopathogenic nematode gathering effect, and over time
The nematode population that prolongation is trapped.When sampling from 48h~96h, the nematode population for trapping is significantly higher than 12,24 and 36h.Show to adopt
This detector optimal sample time is 48h.
4th, detector places impact of the spacing to entomopathogenic nematode gathering effect
Test addition+4 1cm Radix Folium Allii tuberosis of 20 fragrant-flowered garlic maggots in guard, the spacing that detection means is intervally arranged respectively 2,
6th, 10,14,18 and 22cm, the nematode population trapped in investigation kind group detector after inoculation 48h, other test requirements documents are with examination
Test 1.
Result of the test (Fig. 7) shows：Detector places spacing to entomopathogenic nematode gathering effect significant difference, wherein putting
When putting 10~14cm of spacing, trap nematode population and be significantly higher than other placement spacing.Place 2~6cm when, due to detector it
Between it is closer to the distance, easily cause between different detectors compete；And distance is placed in 18~22cm, due to distance between detector
Farther out, the situation that each detector singles solely struggle against is formed, quantity is trapped relatively low.It is when 10~14cm of spacing is placed, each to detect
Good partnership can be formed between device, the energy of fragrant-flowered garlic maggot+Radix Folium Allii tuberosi combination release volatile material and carbon dioxide has been played
Power, improves the gathering effect to nematicide.
5th, fragrant-flowered garlic maggot+Radix Folium Allii tuberosi combination traps the foundation of trend standard curve to entomopathogenic nematode
Under optimal conditions, 6 different administration density are selected, including field maximum application density and less application
Density, detection set up standard curve into the nematode population in detector.50,000,150,000,250,000,35 are separately added in soil
Ten thousandth, 450,000 and 550,000/m2The nematicide suspension of (being respectively equivalent to 0.34 hundred million, 100,000,000,1.6 hundred million, 2.3 hundred million, 300,000,000,3.7/ mu), profit
Reclaimed with apparatus of the present invention, standard is made by linear regression according to actual concentrations gradient and recovery nematode population then
Curve.As shown in figure 8, corresponding linear equation is y=5.138x+0.881, wherein x is abscissa, is be mixed in soil every
Square metre nematicide number, y is vertical coordinate, for the nematicide number for detecting.Coefficient R2=0.9976, show that this device traps effect
Fruit is stable, can meet the detection of field line insect population density.
Field test
In Shandong Province ten thousand Ji Shan proving grounds of fruit tree, soil property is loose and the good sandy loam of aeration for test.
Embodiment 6
The method for being separated and being detected using the entomopathogenic nematode population density detection means of embodiment 1, method is such as
Under：
(1) select to need the plantation Folium Allii tuberosi plot of detection, nematicide 50,000/m of concentration to be applied in Folium Allii tuberosi2, soil moisture
12%, temperature is detected using line insect population device for detecting density at 22 DEG C；
(2) collection tube of different model is installed according to the nematicide in detection different depth soil, by collection tube and seizure pipe
Separate, then will trap material and load in guard, and load in pipe clear water to trapping at hole catch, then will fill catching for clear water
Catch pipe and trap material guard and be installed together with being equipped with；Described traps the combination that material is fragrant-flowered garlic maggot and Radix Folium Allii tuberosi, Radix Folium Allii tuberosi
Length is 1cm；Fragrant-flowered garlic maggot be 3 ages live fragrant-flowered garlic maggot, 20, Radix Folium Allii tuberosi for 1cm Radix Folium Allii tuberosi, 4.
(3) hole is made a call on soil to be detected, then by the seizure pipe being installed together in step (2), collection tube
Entirety is put in hole, interval 10cm arrangement detection means, it is ensured that trap the nematicide of maximum quantity, wherein collection tube is in upper, seizure
Pipe is under；
(4) after placing 48, detection means is fetched from hole, then lays down seizure pipe, and after the water caught in pipe is suctioned out,
Examine under a microscope the number of nematicide in water；
(5) according to the nematicide number observed in step (4), calculate the population density for obtaining nematicide in soil.
Embodiment 7
With separation and the detection method of embodiment 1, other conditions are that what is detected traps with embodiment 1, difference
Material is+4 1cm Radix Folium Allii tuberosis of 5 fragrant-flowered garlic maggots.
Embodiment 8
With separation and the detection method of embodiment 1, other conditions are to place between detector with embodiment 1, difference
Away from for 20cm.
Embodiment 9
With separation and the detection method of embodiment 1, other conditions with embodiment 1, difference are, standing time is
24h。
Comparative example 1
With separation and the detection method of embodiment 1, other conditions are that the method is adopted with embodiment 1, difference
Disclosed in CN101849534A, a kind of entomopathogenic nematode is separated and is carried out with the device in detection means.
Comparative example 2
With separation and the detection method of embodiment 1, other conditions are that the method is adopted with embodiment 1, difference
Disclosed in CN101849534A, a kind of entomopathogenic nematode is separated and is carried out with the device in detection means, and that what is detected traps material
For greater wax moth.
Comparative example 3
With separation and the detection method of embodiment 1, the method is carried out using the device of the present invention, the same embodiment of other conditions
1, difference is that the material that traps of detection is greater wax moth.
Test example
Field test sets 7 process altogether, is detected using apparatus of the present invention detection and CN101849534A disclosed devices respectively
(3 groups of 1 group of embodiment, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment, 1 group of comparative example, 2 groups of comparative example and comparative example).Step
It is respectively adopted described in 1 group of embodiment 1, embodiment 2, embodiment 3, embodiment 4 and comparative example, 2 groups of comparative example, 3 groups of comparative example
Method, plot area 20m2, it is repeated 3 times, each process is repeated 4 times, randomization.Actual conditions step is shown in Table 1.
The method of 1 test process of table
2 different detection means Fields detection Comparative results of table
As can be seen from Table 2, the embodiment 1 that detected using apparatus of the present invention, embodiment 2, embodiment 3, embodiment
4th, detect field nematode population and be significantly higher than CN101849534A disclosed devices comparative example 1 and comparative example 2 with comparative example 3.Adopt
Embodiment 1, embodiment 3, embodiment 4 and the contrast of detection are trapped with 20 fragrant-flowered garlic maggot+Radix Folium Allii tuberosi compositionss of the present invention
The nematode population obtained by example 1 is significantly greater than with greater wax moth as the comparative example 2 and comparative example 3 for trapping thing.Using the present invention
The nematode population obtained by embodiment 1 is significantly higher than 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment, 1 group of comparative example, comparative example
2 groups and 3 groups of comparative example, its absolute error minimum 0.429 is the 6.75 of CN101849534A disclosed devices institute amount detection
Times, show best Detection results.
Claims (10)
1. a kind of entomopathogenic nematode population density detection means, the device include the collection tube of two-layer tubular structure and seizure
Pipe, collection tube include gathering inner tube and collection outer tube two-layer, and collection inner tube and collection outer tube two ends are open settings, collection inner tube
Bottom be threaded with and trap guard, catch pipe positioned at the bottom of collection outer tube and be connected with collection external pipe thread, trap net
Cover in collection outer tube catches pipe for upper end open, the tubular structure of lower end closed, is provided with 2- around the side wall for catching pipe
3 trap hole, be filled with clear water catching in pipe, and the non-woven fabrics that sealing traps hole are provided with the inwall for catching pipe, and
The lower end of non-woven fabrics is extended in clear water.
2. entomopathogenic nematode population density detection means according to claim 1, it is characterised in that collection inner tube and adopt
Spacing between collection outer tube is 2~10mm；Spacing between preferred collection inner tube and collection outer tube is 4~5mm.
3. entomopathogenic nematode population density detection means according to claim 1, it is characterised in that the top of collection tube
It is provided with sealing-plug to gather the seal of tube and collection inner tube and collection outer tube link together；Trap guard height be 15~
20mm, the internal diameter and collection inner tube for trapping guard match.
4. entomopathogenic nematode population density detection means according to claim 1, it is characterised in that catch the height of pipe
For 35~45mm, the internal diameter and collection outer tube of seizure pipe match.
5. entomopathogenic nematode population density detection means according to claim 1, it is characterised in that trap the bottom of guard
End distance traps 1~4mm of hole lower end, and highly preferred, the bottom distance for trapping guard traps hole lower end 2mm；Under non-woven fabrics
It is 4~7mm that end extends to the length of clear water, highly preferred, and it is 5mm that the lower end of non-woven fabrics extends to the length of clear water.
6. entomopathogenic nematode population density detection means according to claim 1, it is characterised in that the height of collection tube
5~60cm；Preferably, the 5~25cm of height of collection tube；Hole is trapped for two, two to trap the spacing between hole be 0.5~2mm.
7. using the entomopathogenic nematode population density detection means detection entomopathogenic nematode population density described in claim 1
Method, including step is as follows：
(1) select to need the plot of detection, to be detected using line insect population device for detecting density；
(2) collection tube of different model is installed according to the nematicide in detection different depth soil, collection tube and seizure pipe is separated,
Then material will be trapped to load in guard, the seizure pipe of clear water will be filled to trapping at hole, then loading clear water in pipe is caught
Be installed together equipped with trapping material guard；Described traps the combination that material is fragrant-flowered garlic maggot and Radix Folium Allii tuberosi, fragrant-flowered garlic maggot and Radix Folium Allii tuberosi
Head number with radical ratio be：(5~25)：(3~6), the length of Radix Folium Allii tuberosi is 0.5~2cm；
(3) hole is made a call on soil to be detected, then by the seizure pipe being installed together in step (2), collection tube entirety
It is put in hole, be intervally arranged detection means, it is ensured that trap the nematicide of maximum quantity, wherein collection tube catches pipe under upper；
(4) after placing 40~50h, detection means is fetched from hole, then lays down seizure pipe, and the water caught in pipe is suctioned out
Afterwards, examine under a microscope the number of nematicide in water；
(5) according to the nematicide number observed in step (4), calculate the population density for obtaining nematicide in soil.
8. it is according to claim 7 detection entomopathogenic nematode population density method, it is characterised in that in step (1)
The humidity of soil to be measured is 10%-18%, and temperature is at 15~35 DEG C.
9. it is according to claim 7 detection entomopathogenic nematode population density method, it is characterised in that step (2) fragrant-flowered garlic
Maggot with the head number of Radix Folium Allii tuberosi with radical ratio is：(15~25)：(4~6), the length of Radix Folium Allii tuberosi is 1cm；It is highly preferred, fragrant-flowered garlic maggot
Head number with Radix Folium Allii tuberosi with radical ratio is：20：4；The fragrant-flowered garlic maggot is the fragrant-flowered garlic maggot living in 3 ages.
10. it is according to claim 7 detection entomopathogenic nematode population density method, it is characterised in that step (3) examine
The spacing that survey device is intervally arranged is 10~14cm, and each process is repeated 4 times and is trapped；Step (4) detection means is in hole
Standing time is 48h.
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