CN103004726A - Simple and effective method for trapping entomopathogenic nematodes - Google Patents
Simple and effective method for trapping entomopathogenic nematodes Download PDFInfo
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- CN103004726A CN103004726A CN2012105829182A CN201210582918A CN103004726A CN 103004726 A CN103004726 A CN 103004726A CN 2012105829182 A CN2012105829182 A CN 2012105829182A CN 201210582918 A CN201210582918 A CN 201210582918A CN 103004726 A CN103004726 A CN 103004726A
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Abstract
The invention discloses a simple and effective method for trapping entomopathogenic nematodes. The method comprises the steps as follows: (1) sleeving an iron wire bag of 10*20 cm with 100 meshes into a cloth bag of 10*20 cm; (2) placing five greater wax moth larvae subjected to diapause treatment in the cloth bag; (3) burying the cloth bag in the position with a distance of 10 cm from the soil surface in a selected place; (4) checking the greater wax moth infection situation every day; and (5) collecting the entomopathogenic nematodes. The method can greatly reduce the trapping workload and the time loss, and has the advantages of simplicity, effectiveness and capability of saving manpower and material resources.
Description
Technical field
The present invention relates to a kind of method for enticing collection, particularly, relate to a kind of method for enticing collection of simple and effective entomopathogenic nematode.
Background technology
Entomopathogenic nematode refers to carry in the body has pathogenic symbiotic bacteria, thereby can cause the class nematode that insect is caused a disease.Entomopathogenic nematode survives in the soil to infect phase worm attitude, and the phase nematode of infecting does not take food, and is independent of the outer free living of insect bodies, seeks or the Invasive insect species host.The mode that pathogenic nematode is sought the host comprises resting form, attack and osculant.Nematode has strict recognition mechanism to insect host, and this identification behavior may be subjected to the impact at 2 time of infection intervals.The recognition mechanism of nematode can prevent that a large amount of nematodes are gathered in the same host body and competition occurs.The nematode infections insect will be through infecting the host, and hiding discharges the deadly three phases of fungal component and toxin and insect, but infect the stage Further Division for looking for the host, identification host and invasion host.Every kind of pathogenic nematode has symbiotic bacteria, and xenorhabdus belongs to
Xenorhabdus(with the genus steinernema symbiosis) and Photobacterium
Photorhabdus(with the mass production symbiosis).Nematode enters in the insect bodies by natural opening or intersegmental membrane, utilizes the protection of pathogenic nematode, and the symbiotic bacteria of carrying in the releaser has avoided the resistance effect of insect to bacterium.After entering in the insect bodies, the intestines endosymbiosis bacterium can be in the blood body cavity of insect amount reproduction, produce toxin and cause insect death, and decompose insect tissue, with as the nematode food source.This cogeneration system is conducive to the biological control insect.
Entomopathogenic nematode is a kind of very ergastic biological control factor that grew up 20 beginnings of the century, is the specialization insect parasite of insect.Many countries are arranged at present in the world, drop into a large amount of man power and material's research and utilization nematodes such as countries such as the U.S., Australia, Germany, New Zealand, Japan, Egypt, be devoted to the nematode production marketing to all over the world.In the biopesticide market of developed country, the market sales revenue of this novel pesticide of entomopathogenic nematode is only second to thuringiensis, oneself is through successfully being applied to prevent and treat agriculture and forestry insect, soil insect and the sanitary insect pest that is difficult to chemical pesticide control, so this new bio insecticide of entomopathogenic nematode has broad application prospects.At present, tens of companies of the U.S., Australia, Holland, Canada and some other countries all can provide nematode for practical application, but the nematode strain that commercialization is produced is above hundred kinds.China has also carried out large quantity research aspect the practical application of entomopathogenic nematode, utilize nematoda control soil dwelling property insect and borer pest all to obtain better control efficiency.Therefore, can estimate that entomopathogenic nematode will play an important role in the pest Biological and ecological control system in future.
China is vast in territory, can estimate that the entomopathogenic nematode resource is very abundant, at present existing part province begins the entomopathogenic nematode data is investigated and developmental research, traditional entomopathogenic nematode method for enticing collection need gather a large amount of soil samples, taking back the laboratory separates and lures collection again, the loss of workload and time is all larger, is unfavorable for carrying out of work.
Summary of the invention
The technical problem to be solved in the present invention is to overcome existing defective, and a kind of simple and effective method for enticing collection is provided, and can effectively save time and manpower by this method.
In order to solve the problems of the technologies described above, the invention provides following technical scheme:
A kind of method for enticing collection of simple and effective entomopathogenic nematode comprises the steps:
(1) 10*20cm, 100 purpose iron wire bags are packed in the cloth bag of 10*20cm, prevent that the greater wax moth cloth bag of breaking by the teeth from escaping;
(2) in cloth bag, place 5 greater wax moth larvas of processing through diapause; Described diapause is treated to 56 ℃ of aqua sterilisas embathes greater wax moth 5 instar larvae 15s, uses immediately from the beginning flowing water drip washing greater wax moth behind the perforated hot water, blots the insect body surface globule with blotting paper after the cooling, for subsequent use;
(3) in selected place cloth bag is embedded in from soil table 10cm depths;
(4) check that greater wax moth is infected situation in the cloth bag every day;
(5) as finding dead greater wax moth is arranged, greater wax moth dead in the bag is put into the dry 36h-48h of sterile petri dish of filter paper after, on the filter paper bar of its horizontally-arranged in catch tray, pour the formalin that concentration is mass ratio 0.1% into; Place under room temperature condition, treat can collect when nematode is swum into formalin, the entomopathogenic nematode that collect this moment is resistance 3 instar larvaes, is stored in 4 ℃ the refrigerator.
Particularly, described method also comprises the check of step (6) entomopathogenic nematode: with the nematode infection greater wax moth or other host insects that lure collection to arrive, this nematode of Observe and measure is to deadly symptom and the pathogenicity of insect; Whether whether observation is dead for examination greater wax moth or other host insects, if dead, have nematode to climb out of in the worm corpse, whether climb out of in the worm corpse if any nematode, infect greater wax moth with the nematode of collecting again, it is dead to observe greater wax moth, if dead, then would be entomopathogenic nematode.
Among the present invention, entomopathogenic nematode also is called for short nematode.
Beneficial effect of the present invention:
Method of the present invention can reduce to lure the workload of collection and the loss of time in a large number, the advantage that have simply effectively, uses manpower and material resources sparingly.
Embodiment
A kind of method for enticing collection of simple and effective entomopathogenic nematode comprises the steps:
(1) 10*20cm, 100 purpose iron wire bags are packed in the cloth bag of 10*20cm, prevent that the greater wax moth cloth bag of breaking by the teeth from escaping;
(2) in cloth bag, place 5 greater wax moth larvas of processing through diapause; Described diapause is treated to 56 ℃ of aqua sterilisas embathes greater wax moth 5 instar larvae 15s, uses immediately from the beginning flowing water drip washing greater wax moth behind the perforated hot water, blots the insect body surface globule with blotting paper after the cooling, for subsequent use;
(3) in selected place cloth bag is embedded in from soil table 10cm depths;
(4) check that greater wax moth is infected situation in the cloth bag every day;
(5) as finding dead greater wax moth is arranged, greater wax moth dead in the bag is put into the dry 36h-48h of sterile petri dish of filter paper after, on the filter paper bar of its horizontally-arranged in catch tray, pour the formalin that concentration is mass ratio 0.1% into; Place under room temperature condition, treat can collect when nematode is swum into formalin, the entomopathogenic nematode that collect this moment is resistance 3 instar larvaes, is stored in 4 ℃ the refrigerator.
Lure after the collection, be check validity, the present invention also comprises the check of step (6) entomopathogenic nematode: with the nematode infection greater wax moth or other host insects that lure collection to arrive, this nematode of Observe and measure is to deadly symptom and the pathogenicity of insect; Whether whether observation is dead for examination greater wax moth or other host insects, if dead, have nematode to climb out of in the worm corpse, whether climb out of in the worm corpse if any nematode, infect greater wax moth with the nematode of collecting again, it is dead to observe greater wax moth, if dead, then would be entomopathogenic nematode.Method of the present invention can reduce to lure workload and the loss of time of collection, the advantage that has simply effectively, uses manpower and material resources sparingly in a large number.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment the present invention is had been described in detail, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. the method for enticing collection of a simple and effective entomopathogenic nematode is characterized in that, comprises the steps:
(1) 10*20cm, 100 purpose iron wire bags are packed in the cloth bag of 10*20cm, prevent that the greater wax moth cloth bag of breaking by the teeth from escaping;
(2) in cloth bag, place 5 greater wax moth larvas of processing through diapause; Described diapause is treated to 56 ℃ of aqua sterilisas embathes greater wax moth 5 instar larvae 15s, uses immediately from the beginning flowing water drip washing greater wax moth behind the perforated hot water, blots the insect body surface globule with blotting paper after the cooling, for subsequent use;
(3) in selected place cloth bag is embedded in from soil table 10cm depths;
(4) check that greater wax moth is infected situation in the cloth bag every day;
(5) as finding dead greater wax moth is arranged, greater wax moth dead in the bag is put into the dry 36h-48h of sterile petri dish of filter paper after, on the filter paper bar of its horizontally-arranged in catch tray, pour the formalin that concentration is mass ratio 0.1% into; Place under room temperature condition, treat can collect when nematode is swum into formalin, the entomopathogenic nematode that collect this moment is resistance 3 instar larvaes, is stored in 4 ℃ the refrigerator.
2. the method for enticing collection of simple and effective entomopathogenic nematode according to claim 1, it is characterized in that, described method also comprises the check of step (6) entomopathogenic nematode: with the nematode infection greater wax moth or other host insects that lure collection to arrive, this nematode of Observe and measure is to deadly symptom and the pathogenicity of insect; Whether whether observation is dead for examination greater wax moth or other host insects, if dead, have nematode to climb out of in the worm corpse, whether climb out of in the worm corpse if any nematode, infect greater wax moth with the nematode of collecting again, it is dead to observe greater wax moth, if dead, then would be entomopathogenic nematode.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106665527A (en) * | 2016-12-19 | 2017-05-17 | 山东省果树研究所 | Entomopathogenic nematodes population density detection device and entomopathogenic nematodes population density detection method |
| CN109832229A (en) * | 2019-02-28 | 2019-06-04 | 山东省果树研究所 | A kind of device and application method detecting the remote diffusivity of entomopathogenic nematode |
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| CN106665527A (en) * | 2016-12-19 | 2017-05-17 | 山东省果树研究所 | Entomopathogenic nematodes population density detection device and entomopathogenic nematodes population density detection method |
| CN109832229A (en) * | 2019-02-28 | 2019-06-04 | 山东省果树研究所 | A kind of device and application method detecting the remote diffusivity of entomopathogenic nematode |
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