CN104381220A - Pollution-free method for controlling bradysia odoriphaga - Google Patents
Pollution-free method for controlling bradysia odoriphaga Download PDFInfo
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- CN104381220A CN104381220A CN201410524793.7A CN201410524793A CN104381220A CN 104381220 A CN104381220 A CN 104381220A CN 201410524793 A CN201410524793 A CN 201410524793A CN 104381220 A CN104381220 A CN 104381220A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/04—Attracting insects by using illumination or colours
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/14—Catching by adhesive surfaces
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M99/00—Subject matter not provided for in other groups of this subclass
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Insects & Arthropods (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a pollution-free method of combining entomopathogenic nematode and black sticky boards for controlling bradysia odoriphaga. During bradysia odoriphaga larval stage, a entomopathogenic nematode suspension liquid with a nematode concentration of 500-1000IJS/mL is applied, and the black sticky boards are suspended in the field for monitoring. When adult bradysia odoriphaga appears, a large number of black sticky boards are suspended, wherein the lower bottom edges of the boards are 0.5-1cm from the ground. 60-80 of the black sticky boards are suspended in each mu of field. According to the controlling method provided by the invention, biological control and physical control are combined. The colored boards are used for trapping and killing adult bradysia odoriphaga, such that pest cardinal number can be effectively reduced. Bradysia odoriphaga larva and adult controlling effects can be improved, and entomopathogenic nematode application amount can be reduced. Therefore, pollution-free, safe, and non-toxic controlling over bradysia odoriphaga pests can be realized.
Description
Technical field
The present invention relates to biological control and physical control technical field, specifically relate to a kind of utilize entomopathogenic nematode and black haftplatte with the use of, the method for efficient, safety, non-environmental pollution control Bradysia odoriphaga.
Background technology
Bradysia odoriphaga is the important pests of harm leek, the liliaceous vegetables such as main harm leek, shallot, onion, spring onion, garlic.Its larva (being commonly called as fragrant-flowered garlic maggot) is mainly clustered in vegetables root, the moth false stem of food and bulb and cause dead seedling.There are for 4 ~ 6 generations at North China open country in this worm, generation overlap serious every year, with larva in leek bulb or around fragrant-flowered garlic root the dormancy of 3 ~ 4cm topsoil survive the winter, spring, season in autumn two occur the heaviest.Due in the cultivation of protecting field leek, temperature and humidity conditions is applicable to growing of fragrant-flowered garlic maggot, therefore it still can continue to endanger leek.
At present, in production, control is still based on chemical insecticide, but effect is not ideal enough, and easily causes environmental pollution, particularly uses phoxim, Imidacloprid and chlopyrifos etc. to carry out filling root.High malicious chemical pesticide or increasing applications of pesticide amount control fragrant-flowered garlic maggot, not only cause its pesticide resistance to increase substantially, also have a strong impact on the edible quality of crop, even occur malicious fragrant-flowered garlic, malicious garlic and malicious green onion etc.Therefore, the nuisanceless technology prevention fragrant-flowered garlic maggot of application is needed badly.Entomopathogenic nematode is the nematode of the special parasitic insect of a class, entered in pin main body by the mode of 3 infective stage larvas in age to the natural opening of insect health or intersegmental membrane intrusion, and discharge the symbiotic bacteria of carrying, make it breed in pest body, produce toxin, finally cause insect dead.Because of entomopathogenic nematode, to have host range extensive, initiatively find host, to non-target organism safety, environment is had no side effect, can be mixed with many agricultural chemicals, fertilizer etc. advantage, be widely used in the multiple agriculture and forestry injurious insects such as control of grubs, cutworm, peach fruit borer, longicorn and fragrant-flowered garlic maggot.
Chinese patent literature CN101779611A (application number 201010112122.1) discloses a kind of propagation method of entomopathogenic nematode, adopt transparent culture vessel as breeding container, adopt yellow mealworm live body as host insect, adopt canebreak as culture medium, sterilizing installation is adopted to carry out sterilizing, temperature control culturing room is used to cultivate, not only obviously solve straw, the waste pollution problem of timber, greatly reduce raw materials for production cost simultaneously, simplify and produce and use step, breed the entomopathogenic nematode cost of turning out low, quality is good, control efficiency is high, can be widely used in preventing and treating agriculture, woods, the soil insect of the plants such as gardening and hiding pest.But compared with chemical insecticide, entomopathogenic nematode insecticidal effect is slow and unstable.Though early-stage Study can prove that nematode has certain control efficiency to fragrant-flowered garlic maggot larva, the insect population radix of adult after sprouting wings effectively can not be controlled.
Colour table trapping is a kind of simple and effective non-environmental pollution control means utilizing insect to develop the taxis of color.Domestic and international research finds, different insect is different to the taxis of color.To yellow and white taxis comparatively by force, black peach aphid, Americal rice leaf miner are addicted to yellow, and diamond-back moth is the most responsive to green for phyllotreta striolata; Bemisia tabaci is the strongest to yellow taxis, is secondly green and red; Palm thrips, citrus orchard thrips and the taxis of Frankliniella occidentalis to blueness are the strongest.At present, production generally use yellow plate carry out Supervise prevention and cure to fragrant-flowered garlic maggot adult.But, yellow be whether this adult addicted to color, need textual criticism.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of method of non-environmental pollution control Bradysia odoriphaga.
Summary of the invention:
The present invention is directed to fragrant-flowered garlic maggot harm wildness and lack the novel pesticide control problem of high-efficiency low-toxicity again, the gathering effect of different colours haftplatte to Bradysia fungus gnat adult is compared by greenhouse and field trial, and carried out the impact that lure collection of colour table on this adult of 3 height, by long-term experiment, reasonably have selected Steinernema Carpocapsae Steinernema fetuae SF-SN entomopathogenic nematode and black haftplatte with the use of carrying out control Bradysia odoriphaga, under field condition, entomopathogenic nematode and black haftplatte compounding application are respectively used to prevent and treat fragrant-flowered garlic maggot larva and fragrant-flowered garlic maggot adult, nematode infection is utilized to prevent and treat fragrant-flowered garlic maggot larva, black haftplatte trapping fragrant-flowered garlic maggot adult, effectively can reduce the insect population radix in field, not only can improve control harm leek, shallot, onion, spring onion, the effect of the vegetable crop that garlic etc. are easily endangered by fragrant-flowered garlic maggot, and reach safe prevention, nuisanceless, the object of environmentally safe.
Detailed Description Of The Invention:
Technical scheme of the present invention is as follows:
A method for non-environmental pollution control Bradysia odoriphaga, comprises step as follows:
(1) soil that is loose and air capacity of soils is selected to carry out wholely, long-term cropping;
(2) use in fragrant-flowered garlic maggot larval phase the entomopathogenic nematode suspension that nematode concentration is 500 ~ 1000IJS/mL, described entomopathogenic nematode suspension is that to meet the entomopathogenic nematode adding distil water of testing conditions formulated, hang the monitoring of black haftplatte in field simultaneously, when haftplatte there is fragrant-flowered garlic maggot adult, start a large amount of suspension black haftplatte, the bottom distance ground 0.5 ~ 1cm of black haftplatte, long limit is parallel to the ground, every mu is hung black haftplatte number is 60 ~ 80 pieces, carries out normal field management after using;
Above-mentionedly meet the requirement that testing conditions should meet following a:
A: entomopathogenic nematode adding distil water is mixed with the entomopathogenic nematode suspension that nematode concentration is 500 ~ 1000IJS/mL, under the condition of 24 ~ 26 DEG C, place 24h, 48h, 72h detect, entomopathogenic nematode survival rate is more than 98%.
The present invention is preferred, and described entomopathogenic nematode is Steinernema Carpocapsae Steinernema fetuae SF-SN.
Preferred according to the present invention, carry out the winter ploughing winter before whole ground in step (1) and execute, eliminate fragrant-flowered garlic maggot larva or clean field, reduce the generation radix of fragrant-flowered garlic maggot.
Preferred according to the present invention, in step (1), crop is that leek, shallot, onion, spring onion, garlic etc. are easily by liliaceous vegetable crop that fragrant-flowered garlic maggot endangers.
The present invention is preferred, and step (2) uses entomopathogenic nematode suspension at fragrant-flowered garlic maggot Larvae occurrence time, hangs black haftplatte simultaneously, and when on black haftplatte, fragrant-flowered garlic maggot adult number is more than or equal to 1, every mu is hung black haftplatte number is 60 ~ 80 pieces.By long term test, the long limit gathering effect to adult parallel to the ground of black haftplatte is best, increases the chance luring integrated worm, improves the control efficiency of insect pest.
Loose and that air capacity of soils is good soil is selected in described step (1), its object is to for entomopathogenic nematode survival, seek, infect host and set up the environment that Natural Population provides favourable, therefore those skilled in the art can according to above-mentioned purpose conveniently technology select.
Preferred according to the present invention, in described step (2) entomopathogenic nematode suspension use in the following way: adopt the mode of irrigating that sprays or directly water to use.
Preferred further, described spraying adopts sprayer to spray entomopathogenic nematode suspension 2 ~ 3 times, sprays in the scope of distance rhizome portion, ground 3 ~ 20cm, every mu of amount of application 60 ~ 80L.
Preferred according to the present invention, in step (2), fragrant-flowered garlic maggot larval phase, refers to the fragrant-flowered garlic maggot in below fragrant-flowered garlic maggot 3 age.
Preferred according to the present invention, in described step (2), nematode consumption is every mu of amount of application 1 ~ 200,000,000.
Preferred according to the present invention, the entomopathogenic nematode in described step (2) is these entomopathogenic nematode product of Steinernema fetuae SF-SN is this area conventional variety, can be obtained by common commercial approach.
Black haftplatte in step (2) is so enough that also can to prepare voluntarily by market, Jia Duo science, industry and trade Co., Ltd is on sale, the present invention is preferred, described black haftplatte material selection plastic plate, coated at the surface black plastic film of plastic plate, then even spread black viscose glue is made, and profile is rectangle, length is 20 ~ 30cm, and wide is 10 ~ 15cm.
Distance between preferred black haftplatte of the present invention is 2 ~ 3 meters.
Technical characterstic of the present invention and advantage:
1, the present invention adopts entomopathogenic nematode SF-SN and black haftplatte compounding application to be respectively used to prevent and treat fragrant-flowered garlic maggot larva and fragrant-flowered garlic maggot adult, nematode infection is utilized to prevent and treat fragrant-flowered garlic maggot larva, the object of black haftplatte trapping fragrant-flowered garlic maggot adult, effectively can reduce the insect population radix in field, vermins-proof effect compares independent entomopathogenic nematode SF-SN and improves 43.40 ~ 49.78% respectively with black haftplatte separately, and the usage amount and the black haftplatte that greatly reduce entomopathogenic nematode SF-SN hang quantity, when nematode suspension concentration is 500 ~ 1000IJS/mL, every mu of nematode amount of application 1 ~ 200,000,000 (density being applicable to fragrant-flowered garlic maggot is 200,000 ~ 500,000/mu), it is 60 ~ 80 pieces/mu that black haftplatte hangs number, and entomopathogenic nematode is when being Steinernema Carpocapsae Steinernema fetuae SF-SN, control fragrant-flowered garlic maggot reaches 94.66 ~ 97.19%.Bright by long term test summary analysis, below fragrant-flowered garlic maggot larva 3 age, use nematode SF-SN suspension in field, and hang black haftplatte in the adult stage, significantly can decrease the usage amount of often kind of material, both cost-saving, reach again the object of safety, high-efficiency prevention and control fragrant-flowered garlic maggot.The present invention simultaneously can replace the insecticide pesticide kinds such as high malicious high residue, not only has ecological significance, also has important environment protection significance simultaneously.
2, biological control combines with physical control by prevention and controls of the present invention, namely research entomopathogenic nematode and colour table fit applications reach nematoda control fragrant-flowered garlic maggot larva, colour table trapping fragrant-flowered garlic maggot adult, thus effectively reduce the object of insect population radix, the control efficiency to fragrant-flowered garlic maggot larva and adult can not only be improved, and the usage amount of entomopathogenic nematode can be reduced, finally realize Bradysia odoriphaga insect non-environmental pollution control target.
Accompanying drawing explanation
Fig. 1 is the gathering effect block diagrams of 10 kinds of color haftplattes to fragrant-flowered garlic maggot adult, and in figure, different capitalization represents remarkable through DuncanShi new multipole difference method inspection P<0.01 level error heteropole respectively.Fig. 2 is the gathering effect block diagram of differing heights colour table to fragrant-flowered garlic maggot adult, and in figure, after equal height, after different capitalization, same colour table, different lowercase represents remarkable through DuncanShi new multipole difference method inspection P<0.01 level error heteropole respectively.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further elaborated, but institute of the present invention protection domain is not limited thereto.
Laboratory test measures
1, entomopathogenic nematode SF-SN is to the pathogenicity of different larval instar fragrant-flowered garlic maggot larva
Entomopathogenic nematode Steinernema fetuae SF-SN (being called for short SF-SN), for entomopathogenic nematode is commonly used in this area, can be obtained by common commercial approach or gather acquisition voluntarily, nematode SF-SN is by taking SF-SN as kind, propagation method breeding with reference to the disclosed entomopathogenic nematode of Chinese patent literature CN101779611A (application number 201010112122.1) obtains, and SF-SN kind is obtained by Guangdong Province Insects Research Institute.
Adopt culture dish filter paper method, measure entomopathogenic nematode to the pathogenicity of different larval instar fragrant-flowered garlic maggot larva.5 series concentration are diluted to each for examination nematode suspension by geometric ratio method distilled water.In the culture dish of diameter 9cm, put two Medium speed filter papers, drip each treatment fluid 1.5mL respectively, put into 20 3 age fragrant-flowered garlic maggot and appropriate leek stem section, often 4 repetitions are established in process, do blank with distilled water process.Be placed in the incubator of temperature (22 ± 1) DEG C, humidity (60 ± 10) %.Because of fragrant-flowered garlic maggot happiness dark surrounds, therefore in culture dish peripheral closure black cloth, avoid fragrant-flowered garlic maggot to creep outside ware.The little writing brush point of process 3d touches polypide, and motionless person is designated as death, calculates corrected mortality, obtains virulence regression equation.
Entomopathogenic nematode SF-SN to 3 ages and 4 age fragrant-flowered garlic maggot larva pathogenicity in table 1.In 3 length of times, larval mortality reaches 50% and 95% only needs SF-SN nematode 60.0/worm and 981.8/worm; To 4 length of times, cause equal lethality then to need 236.6/worm and 1405.7/worm, nematode population increases by 1.43 ~ 3.94 times.Illustrate that the susceptibility of fragrant-flowered garlic maggot larva to nematode progressively declines with the length of time and body weight increase.
Table 1SF-SN strain is to the virulence of fragrant-flowered garlic maggot different larval instar larva
| The length of time | Virulence regression equation | LD 50 | LD 95 | LD 50Confidence limit | Correlation coefficient |
| 3 | 1.3703x+2.5634 | 60.0 | 981.8 | 36.5-98.5 | 0.9994 |
| 4 | 2.1408x+0.0942 | 236.6 | 1405.7 | 145.5-1009.4 | 0.9120 |
Field trial
1, different colours haftplatte is to fragrant-flowered garlic maggot adult gathering effect
Test and carry out at paradise, Feicheng township, Shandong Province Vegetable Base in the 2-5 month in 2014, this garden plantation leek kind is that the only son and heir is red.By black, red, powder, Huang, purple, indigo plant, pale blue, green, grey and white 10 kinds of different colours haftplattes, haftplatte specification: 22cm × 12cm, evenly distributed in leek land for growing field crops and green house by random district group respectively, the bottom distance ground 1cm of haftplatte, often process repetition 6 times, the quantity of Bradysia odoriphaga adult on the every colour table of 3d " Invest, Then Investigate ".
Result of the test (see Fig. 1) shows, after 3d, in land for growing field crops and green house, the gathering effect of 10 kinds of color sliders to fragrant-flowered garlic maggot adult is different, but gathering effect basic simlarity, black is best, and purple is the poorest.Analysis of variance, in green house, there is pole significant difference in black and red, powder, Huang, purple, indigo plant, pale blue, 9 kinds of colors such as green, grey and white, but without significant difference between these 9 kinds of colors.And under field condition, black and other 9 kinds of colors are same exists pole significant difference, but red, powder, purple, indigo plant, pale blue, between ash and white, difference is not remarkable.Therefore, from these 10 kinds of colors, choose the good 6 kinds of colors of gathering effect, proceed test.
2, differing heights colour table lures worm effect assessment
That best for gathering effect 6 kinds of colors are carried out diverse location lures worm effect assessment.3 height are set, are respectively: top: the bottom distance ground 30cm of colour table; Middle part: the bottom distance ground 15cm of colour table; Bottom: the bottom distance ground 1cm of colour table.Concordant with ground distance ground 1cm, 15cm and 30cm totally 3 kinds of differing heights hang colour tables, often process repetitions 6 times, the quantity of Bradysia fungus gnat adult on every plate after investigation 3d.
Result of the test (see Fig. 2) shows, fragrant-flowered garlic maggot adult quantity variance differing heights position colour table luring collection arrive is very large, along with highly rising, lure worm amount to reduce gradually, its gathering effect is followed successively by: 1cm>15cm>30cm.Analysis of variance, the gathering effect pole of colour table to fragrant-flowered garlic maggot adult of lower edge overhead 1cm is significantly higher than 15cm, 30cm.Mutually level colour table is in the trapping test of this adult, and the gathering effect of the blackboard at 1cm and 15cm place is all significantly higher than ash, indigo plant, green, pale blue and yellow plate.Therefore the gathering effect of blackboard to fragrant-flowered garlic maggot adult of the bottom distance ground 1cm of colour table is better.
3, highly pathogenicity nematode strain coordinates the preventive effect that should have fragrant-flowered garlic maggot with black haftplatte
Entomopathogenic nematode Steinernema carpocapsae NC116 (being called for short NC116) Steinernema carpocapsaeAll (being called for short All).Above-mentioned entomopathogenic nematode is this area and commonly uses entomopathogenic nematode, can be obtained by common commercial approach or gather acquisition voluntarily, nematode NC116 is by taking NC116 as kind, propagation method breeding with reference to the disclosed entomopathogenic nematode of Chinese patent literature CN101779611A (application number 201010112122.1) obtains, nematode All is by taking All as kind, propagation method breeding with reference to the disclosed entomopathogenic nematode of Chinese patent literature CN101779611A (application number 201010112122.1) obtains, NC116 kind and All kind are obtained by Guangdong Province Insects Research Institute.
Alone nematode process is established in test, and alone colour table lures collection process, nematode and the process of colour table fit applications and blank.(area is 0.1256m to each process 4 basin
2), in basin, sandy soil ratio is 1:4, every pot transplanting 8-10 strain leek.Access fragrant-flowered garlic maggot 3 instar larvae 100/basin, 80 object fly nets on every basin cover.Using nematode dosage is 200IJs/ head, and colour table height is colour table lower edge overhead 1cm place.Process 5d, investigates the larva quantity in every basin, calculates different lines nematode to the preventive effect of larva.Process 15d investigates in every basin net and becomes borer population, calculates different lines nematode and colour table and lures collection with the use of to adult decline rate and preventive effect.
Table 2 different disposal is to fragrant-flowered garlic maggot larva and the potted plant preventive effect of adult
Do not represent remarkable through DuncanShi new multipole difference method inspection P<0.01 and P<0.05 level error heteropole with the large and small mother stock that writes different after column data.
Result shows (table 2), and different lines nematode is different with the control efficiency of adult to fragrant-flowered garlic maggot larva, and SF-SN strain control efficiency is the highest, and All strain is taken second place, and NC116 strain is the poorest.Wherein use nematode 5d, SF-SN strain is 60.26% to the control efficiency of larva, blackboard to the control efficiency of fragrant-flowered garlic maggot adult and SF-SN strain differences not remarkable.Nematode to the process of preventing and treating of fragrant-flowered garlic maggot adult mainly nematode parasitism is formed to larva in soil and pupa, and blackboard is mainly owing to having trapping effect to fragrant-flowered garlic maggot adult.When blackboard and nematode with the use of time, the preventive effect of fragrant-flowered garlic maggot adult is significantly higher than to nematode is alone and blackboard is alone, wherein SF-SN nematode+blackboard with the use of, its control efficiency be significantly higher than other two kinds of strains and blackboard with the use of, 97.60% is reached to the control efficiency of adult, greatly can reduce the adult radix of fragrant-flowered garlic maggot, the control of fragrant-flowered garlic maggot is played a key effect.
Field experiment is verified
Bradysia odoriphaga is the important pests of harm leek, the liliaceous vegetables such as main harm leek, shallot, onion, spring onion, garlic.This test selects leek to carry out field trial checking.Test is at paradise, Feicheng township, Shandong Province Vegetable Base, and soil property is loose and that air capacity of soils is good sandy loam.Leek kind is that the only son and heir is red, the quantity of Pre-trial Investigation leek ground fragrant-flowered garlic maggot larva, and select the basically identical plot of fragrant-flowered garlic maggot quantity to test, each plot area is 40m
2, between different disposal, isolated area is set, repeats 4 times, randomized arrangement.Near leek plant, pour into nematode, nematode usage amount is 60kg nematode suspension/mu.Colour table suspension height is colour table lower edge overhead 1cm place.Respectively at using rear 5d, 30d investigation of process to first brood of larvae and second generation larva control efficiency.The entomopathogenic nematode Steinernema carpocapsae SF-SN of above-mentioned screening is utilized to test.In suspension, nematode concentration is 500 ~ 1000IJS/mL.Every mu of nematode amount of application 1 ~ 200,000,000, black insect-sticking plate (22cm × 12cm) long limit is parallel to the ground, and it hangs number is 60 ~ 80 pieces/mu.
Embodiment 1
Adopt and prevent and treat Bradysia odoriphaga with the following method,
(1) the winter ploughing winter executes, and eliminates fragrant-flowered garlic maggot larva or clean field, reduces the generation radix of fragrant-flowered garlic maggot, and select soil that is loose and air capacity of soils to carry out wholely, plantation leek, leek kind is that the only son and heir is red;
(2) the fragrant-flowered garlic maggot Larvae occurrence time below 3 ages, entomopathogenic nematode suspension is poured into the mode of irrigating of directly watering near leek plant, in entomopathogenic nematode suspension, nematode concentration is 800IJS/mL, nematode consumption is 100,000,000/mu (density being applicable to fragrant-flowered garlic maggot is 200,000 ~ 500,000/mu), kills fragrant-flowered garlic maggot larva; Hang the monitoring of black haftplatte in field simultaneously, when on black haftplatte, fragrant-flowered garlic maggot adult number is more than or equal to 1, start a large amount of suspension black haftplatte, the bottom distance ground 1cm of black haftplatte, and by parallel to the ground for the long limit of black haftplatte, every mu is hung black haftplatte number is 60 pieces, carries out normal field management after using; Entomopathogenic nematode is Steinernema Carpocapsae Steinernema fetuae SF-SN, and black haftplatte material selection plastic plate is coated at the surface black plastic film of plastic plate, then even spread black viscose glue is made, profile is rectangle, and length is: 22cm, is widely: 12cm.
Embodiment 2
Other conditions are with embodiment 1, and difference is, entomopathogenic nematode consumption is 1.5 hundred million/mu (density being applicable to fragrant-flowered garlic maggot is 200,000 ~ 500,000/mu), and every mu is hung black haftplatte number is 70 pieces.Normal field management is carried out after using.
Embodiment 3
Other conditions are with embodiment 1, and difference is, entomopathogenic nematode consumption is 200,000,000/mu (density being applicable to fragrant-flowered garlic maggot is 200,000 ~ 500,000/mu), and every mu is hung black haftplatte number is 80 pieces.Normal field management is carried out after using.
Embodiment 4
Other conditions are with embodiment 1, and difference is, spray in the scope of distance rhizome portion, ground 20cm, every mu of amount of application 60L.Normal field management is carried out after using.
Comparative example 1
Other conditions are with embodiment 1, and difference is, entomopathogenic nematode adopts entomopathogenic nematode NC116, and its nematode consumption is 200,000,000/mu, and every mu is hung black haftplatte number is 80 pieces.Normal field management is carried out after using.
Comparative example 2
Other conditions are with embodiment 1, and difference is, entomopathogenic nematode adopts entomopathogenic nematode All, and its nematode consumption is 200,000,000/mu, and every mu is hung black haftplatte number is 80 pieces.Normal field management is carried out after using.
Test example
Field trial establishes 10 process altogether, be alone black haftplatte (black haftplatte group, 160 piece/mu), alone nematode (All nematode group, SF-SN nematode group, NC116 nematode group) respectively, hang black haftplatte and use nematode (embodiment 1 group, embodiment 2 groups, embodiment 3 groups, comparative example 1 group and comparative example 2 groups) and blank (control group).Wherein, hang black haftplatte and adopt embodiment 1, embodiment 2, embodiment 3, comparative example 1 and the method described in comparative example 2 with the step using nematode, adopt " Z " shape sampling method to investigate 5 points in every community, often some investigation 15 strains.
Actual conditions step is in table 3.
The method of process tested by table 3
| Sequence number | Process | Enforcement amount |
| 1 | Black haftplatte group | 160 pieces/mu |
| 2 | All nematode group | 400000000/mu |
| 3 | NC116 nematode group | 400000000/mu |
| 4 | SF-SN nematode group | 400000000/mu |
| 5 | Embodiment 1 group | SF-SN nematode 100,000,000/mu+black haftplatte 60 pieces/mu |
| 6 | Embodiment 2 groups | SF-SN nematode 1.5 hundred million/mu+black haftplatte 70 pieces/mu |
| 7 | Embodiment 3 groups | SF-SN nematode 200,000,000/mu+black haftplatte 80 pieces/mu |
| 8 | Comparative example 1 group | NC116 nematode 200,000,000/mu+black haftplatte 80 pieces/mu |
| 9 | Comparative example 2 groups | All nematode 200,000,000/mu+black haftplatte 80 pieces/mu |
| 10 | Control group | Clear water |
Table 4 entomopathogenic nematode SF-SN and black haftplatte are with the use of field control effect
As can be seen from Table 4,5d after process, for stage of first larvae, now only have nematode to play a role, be totally worse than and use so embodiment 1, embodiment 2, embodiment 3, comparative example 1 and comparative example 2 prevent and treat fragrant-flowered garlic maggot first brood of larvae effect the preventive effect that nematode concentration is All, NC116 and SF-SN nematode of 400,000,000/mu.But embodiment 1, embodiment 2, embodiment 3, comparative example 1 and comparative example 2 are when stage of first larvae and adult stage, black haftplatte and nematode play a role jointly, reduce first generation adult insect population radix, egg laying amount reduces, so after treatment 30d time, the insect population radix of second generation larva obviously reduces, and serves the effect of insect protected.Although early stage can not play a role to the control of fragrant-flowered garlic maggot, can obviously reduce insect population radix in the control in later stage, the vermins-proof effect of embodiment 1, embodiment 2, embodiment 3 is more alone than both improves 43.40 ~ 49.78% respectively.Summary analysis is bright used nematode SF-SN before fragrant-flowered garlic maggot larva 3 age (comprising for 3 ages) in field, and the adult stage hang black haftplatte with the use of, vermins-proof effect reaches 94.66 ~ 97.19%.
Claims (10)
1. a method for non-environmental pollution control Bradysia odoriphaga, comprises step as follows:
(1) soil that is loose and air capacity of soils is selected to carry out wholely, long-term cropping;
(2) use in fragrant-flowered garlic maggot larval phase the entomopathogenic nematode suspension that nematode concentration is 500 ~ 1000IJS/mL, described entomopathogenic nematode suspension is that to meet the entomopathogenic nematode adding distil water of testing conditions formulated, hang the monitoring of black haftplatte in field simultaneously, when haftplatte there is fragrant-flowered garlic maggot adult, start a large amount of suspension black haftplatte, the bottom distance ground 0.5 ~ 1cm of black haftplatte, long limit is parallel to the ground, every mu is hung black haftplatte number is 60 ~ 80 pieces, carries out normal field management after using;
Above-mentionedly meet the requirement that testing conditions should meet following a:
A: entomopathogenic nematode adding distil water is mixed with the entomopathogenic nematode suspension that nematode concentration is 500 ~ 1000IJS/mL, under the condition of 24 ~ 26 DEG C, place 24h, 48h, 72h detect, entomopathogenic nematode survival rate is more than 98%.
2. the method for non-environmental pollution control Bradysia odoriphaga according to claim 1, is characterized in that, described entomopathogenic nematode is Steinernema Carpocapsae Steinernema fetuae SF-SN.
3. the method for non-environmental pollution control Bradysia odoriphaga according to claim 1, is characterized in that, carries out the winter ploughing winter and execute in step (1) before whole ground, eliminates fragrant-flowered garlic maggot larva or clean field, reduces the generation radix of fragrant-flowered garlic maggot.
4. the method for non-environmental pollution control Bradysia odoriphaga according to claim 1, is characterized in that, crop is that leek, shallot, onion, spring onion, garlic etc. are easily by liliaceous vegetable crop that fragrant-flowered garlic maggot endangers in step (1).
5. the method for non-environmental pollution control Bradysia odoriphaga according to claim 1, it is characterized in that, step (2) uses entomopathogenic nematode suspension at fragrant-flowered garlic maggot Larvae occurrence time, hang black haftplatte simultaneously, when on black haftplatte, fragrant-flowered garlic maggot adult number is more than or equal to 1, every mu is hung black haftplatte number is 60 ~ 80 pieces.
6. the method for non-environmental pollution control Bradysia odoriphaga according to claim 1, is characterized in that, in described step (2) entomopathogenic nematode suspension use in the following way: adopt the mode of irrigating that sprays or directly water to use.
7. the method for non-environmental pollution control Bradysia odoriphaga according to claim 6, it is characterized in that, described spraying adopts sprayer to spray entomopathogenic nematode suspension 2 ~ 3 times, sprays in the scope of distance rhizome portion, ground 3 ~ 20cm, every mu of amount of application 60 ~ 80L.
8. the method for non-environmental pollution control Bradysia odoriphaga according to claim 1, is characterized in that, fragrant-flowered garlic maggot larval phase refers to the fragrant-flowered garlic maggot in below fragrant-flowered garlic maggot 3 age in step (2).
9. the method for non-environmental pollution control Bradysia odoriphaga according to claim 1, is characterized in that, in described step (2), nematode consumption is every mu of amount of application 1 ~ 200,000,000.
10. the method for non-environmental pollution control Bradysia odoriphaga according to claim 1, it is characterized in that, described black haftplatte material selection plastic plate, coated at the surface black plastic film of plastic plate, then even spread black viscose glue is made, profile is rectangle, and length is 20 ~ 30cm, and wide is 10 ~ 15cm.
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