CN106234369B - A kind of bactericidal composition of alkene containing benzo fluorine bacterium azoles and amino-oligosaccharide - Google Patents

A kind of bactericidal composition of alkene containing benzo fluorine bacterium azoles and amino-oligosaccharide Download PDF

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Publication number
CN106234369B
CN106234369B CN201610636569.6A CN201610636569A CN106234369B CN 106234369 B CN106234369 B CN 106234369B CN 201610636569 A CN201610636569 A CN 201610636569A CN 106234369 B CN106234369 B CN 106234369B
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Prior art keywords
oligosaccharide
amino
bacterium azoles
fluorine bacterium
bactericidal composition
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CN201610636569.6A
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CN106234369A (en
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李祥英
张敬敬
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Suzhou Yishuimo Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/561,2-Diazoles; Hydrogenated 1,2-diazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a kind of bactericidal compositions of alkene containing benzo fluorine bacterium azoles and amino-oligosaccharide, wherein benzo alkene fluorine bacterium azoles and the weight ratio of amino-oligosaccharide are 1:40‑40:1, composition of the invention can be processed into a variety of dosage forms by conventional preparation processing method, be mainly used for preventing crop powdery mildew.

Description

A kind of bactericidal composition of alkene containing benzo fluorine bacterium azoles and amino-oligosaccharide
Technical field
The present invention relates to composition pesticide fields, and in particular to be a kind of fluorine bacterium azoles of alkene containing benzo and amino-oligosaccharide Bactericidal composition.
Invention content
Powdery mildew is a kind of worldwide disease occurred extensively, and chemical pesticide control powdery mildew, mesh are mainly used in production Before rely primarily on benzimidazole, triazole type, methoxy acrylic bactericide.However, killing for single kind is used for a long time Microbial inoculum can make disease generate different degrees of resistance, and control effect reduces.
Benzo alkene fluorine bacterium azoles are a kind of pyrazole amide series bactericidal agents, by inhibiting on mitochondrial inner membrane in electron transport chain Succinate dehydrogenase so that disease fungus can not generate energy via breathing, and then disintegrate the growth of germ, and the compound is independent Higher operating costs and the generation for being easy hair resistance again, to reduce the service life of pesticide species.
Amino-oligosaccharide(Chitosan oligosaccharide)Refer to D- Glucosamines with the oligosaccharide of β -1.4 glucosides key connections, by chitin Say less toxic fungicide that is obtained, or being extracted by microbial fermentation of degrading again after solving chitosan.Amino-oligosaccharide can be to some diseases The growth of bacterium generates inhibiting effect, influences the sprouting of fungal spore, hypha form morphs, biochemistry changes in spore for induction Deng.Gene in energy activated plant body, generate the chitinase with resistant effect, dextranase, protect element and PR albumen etc., and have Cell activation acts on, and contributes to the recovery of aggrieved plant, promoting root growth strong sprout to enhance the resistance of crop, promotes growth and development of plants. Amino-oligosaccharide solution has antivirus, bactericidal, fungicidal action.Not only to bacterium, fungi, virus have extremely strong prevention and Eradicant action, but also have the function of nutrition, adjusting, removing toxic substances, antibacterial.Not only fungi, bacterium, virus are prevented with extremely strong It controls and eradicant action, but also has effects that nutrition, adjusting, removing toxic substances, antibacterial.It can be widely used for prevention fruit tree, vegetables and cotton The diseases such as mosaic disease, powdery mildew, little leaf, spot disease, anthracnose, downy mildew, epidemic disease caused by grain crop.
Present inventor has made intensive studies the recipe determination of benzo alkene fluorine bacterium azoles and amino-oligosaccharide, finds Benzo alkene fluorine bacterium azoles and amino-oligosaccharide carry out mixture has apparent synergistic work to a variety of fungies in a certain range With completing the present invention after further study.
It there is no the relevant report of the complex composition of benzo alkene fluorine bacterium azoles and amino-oligosaccharide at present.
Invention content
The object of the present invention is to provide a kind of Synergistic bactericidal composition for being suitble to agriculturally use, helps to reduce and use Dose, the generation for delaying pathogen resistance and reduction use cost.
In order to solve the above technical problems, the present invention adopts the following technical scheme that:A kind of Synergistic bactericidal composition, feature exist In active constituent contains benzo alkene fluorine bacterium azoles and amino-oligosaccharide, wherein the mass ratio of benzo alkene fluorine bacterium azoles and amino-oligosaccharide It is 1:40-40:1, preferably 1:10-10:1.
Further, the weight ratio of active constituent is 1-90%, preferably 10-70% in the bactericidal composition.
Further, the bactericidal composition can be processed into agricultural by preparation method conventional in Pesticide formulation The arbitrary dosage form of upper permission, such as can be wettable powder, water dispersible granules, aqueous emulsion, microemulsion.
It is that each substance has mixed in composition that the composition of the present invention can be provided in the form of finished product preparation, can also be with Unit dose form provides, and is directly mixed in proportion in bucket or tank using preceding, is then diluted to required concentration.
Further, composition of the invention can also be added other agricultural active ingredients, for example, fungicide, insecticide, The agricultural actives ingredient such as herbicide, fertilizer.
The bactericidal composition of the present invention is suitable for preventing and kill off a variety of diseases such as powdery mildew of cucumber, tomato powdery mildew, powdery mildew of strawberry Evil.
Compared with prior art, composition pesticide of the invention has the beneficial effect that:
(1)After the composition compounding of the present invention, have obvious synergistic function in a certain range, preventive effect and list A active constituent is compared and is significantly improved;
(2)The active constituent mechanism of action is different in the composition of the present invention, while effectively prevention crop disease, The generation that can delay pathogen drug resistance is conducive to the service life for delaying pesticide activity component;
(3)The composition preparation process of the present invention is simple, is suitble to promote and apply in actual production.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, present invention following specific examples It illustrates, but the present invention is limited to absolutely not these examples.It is as described below to be only the preferred embodiment of the present invention, it is used only for explaining The present invention, but cannot be therefore understands that for the limitation to the scope of the claims of the present invention.It should be pointed out that it is all the present invention spirit and Any modification, equivalent replacement or improvement etc., should all be included in the protection scope of the present invention made by within principle, therefore, The protection domain of patent of the present invention should be determined by the appended claims.
One, example of formulations
1, embodiment 1:41% benzo alkene fluorine bacterium azoles amino-oligosaccharide water dispersible granules(40:1)
Benzo alkene fluorine bacterium azoles 40%
Amino-oligosaccharide 1%
Sodium lignin sulfonate 4%
Lauryl sodium sulfate 6%
Polyvinyl alcohol 2%
White carbon 1%
Diatomite surplus
2, embodiment 2:21% benzo alkene fluorine bacterium azoles amino-oligosaccharide wettable powder(20:1)
Benzo alkene fluorine bacterium azoles 20%
Amino-oligosaccharide 1%
Lauryl sodium sulfate 3%
Sodium lignin sulfonate 2%
Pull open powder 3%
Diatomite surplus
3, embodiment 3:25% benzo alkene fluorine bacterium azoles amino-oligosaccharide suspending agent(23:2)
Benzo alkene fluorine bacterium azoles 23%
Amino-oligosaccharide 2%
Sodium lignin sulfonate 8%
Octyl phenol polyglycol ether 3%
Xanthans 1%
Glycerine 6%
Deionized water surplus
4, embodiment 4:10% benzo alkene fluorine bacterium azoles amino-oligosaccharide aqueous emulsion(1:1)
Benzo alkene fluorine bacterium azoles 5%
Amino-oligosaccharide 5%
Lauryl sodium sulfate 3%
N-Methyl pyrrolidone 7%
Isobutanol 9%
Polyoxyethylene fatty alcohol 2%
Alkyl polyglycol ether 6%
Deionized water surplus
Two, Toxicity Determination
Subjects:Cucumber powdery mildew's pathogen
Test method:Experiment refers to People's Republic of China (PRC) professional standard NY/T 1156.11-2008《Pesticide indoor biological Measure the 11st part of test rule fungicide:It prevents cucurbits powdery mildew and tests pot-culture method》, measured using pot-culture method.
Susceptible cucumber variety is sowed, it is spare that potting grows to 2 leaf periods.By prepared benzo alkene fluorine bacterium azoles and amino Oligosaccharide mother liquor is dissolved with 0.1% Tween-80 aqueous surfactant solution and prepares concentration gradient.And it sets containing only solvent and surface Activating agent and without containing effect ingredient processing make blank control.24 h after chemicals treatment, spray inoculation cucumber powdery mildew's pathogen spore Suspension.The process for preparation of spore suspension is as follows:It takes and covers with Powdery Mildew blade, with a small amount of Tween-80 surface-active of refueling The sterile water of agent washes down spore, double gauze filtering, and 1 × 10 is modulated into after microscopy5The suspension of a/mL.Inoculation is placed on In thermostatic chamber, cultivated 7 days under the conditions of 24 DEG C of temperature.When blank control disease liquid rate reaches 80% or more, each processing is investigated in classification Incidence calculates the disease index and control effect of each processing.
The synergy situation of two kinds of medicaments is calculated according to colby formula, test result is as shown in table 1.
The Toxicity Determination result shows benzo alkene fluorine bacterium azoles and amino-oligosaccharide weight ratio 1:40-40:1 range It is interior, 120 are all higher than to the co-toxicity coefficient of powdery mildew of cucumber prevention, shows the weight of synergistic function, especially composition Than 1:10-10:When 1, co-toxicity coefficient is all higher than 450, and synergistic effect becomes apparent from.
Three, field control effectiveness test
In order to further verify the practical application effect of the present invention, prevention cucumber is carried out using the medicament of the embodiment of the present invention Powdery mildew field control effectiveness test.
Reagent agent:(1)41% benzo alkene fluorine bacterium azoles amino-oligosaccharide water dispersible granules(40:1);(2)21% benzo alkene Fluorine bacterium azoles amino-oligosaccharide wettable powder(20:1);(3)25% benzo alkene fluorine bacterium azoles amino-oligosaccharide suspending agent(23: 2);(3)10% benzo alkene fluorine bacterium azoles amino-oligosaccharide aqueous emulsion(1:1).
It 5 days after medicine, investigates within 10 days disease indexs and calculates preventive effect, test result is shown in Table 2.
Field control effectiveness test result show the complex composition preparation of embodiment 1-4 powdery mildew of cucumber is shown compared with Good control effect, lower in dosage, preventive effect is still better than the preventive effect of single active constituent.In addition, in medication In range, composition of the invention does not find to generate poisoning to crop, and compared with single dose, cucumber growth is more healthy and strong, is worth It is promoted and applied in production.

Claims (5)

1. a kind of bactericidal composition, which is characterized in that active constituent includes benzo alkene fluorine bacterium azoles and amino-oligosaccharide, wherein benzene And alkene fluorine bacterium azoles and the weight ratio of amino-oligosaccharide are 1:40-40:1.
2. bactericidal composition according to claim 1, which is characterized in that the benzo alkene fluorine bacterium azoles and amino-oligosaccharide Weight ratio is preferably 1:10-10:1.
3. bactericidal composition according to claim 1 or 2, which is characterized in that the weight of active constituent in the composition Percentage composition is 0.1-80%.
4. bactericidal composition according to claim 1 or 2, which is characterized in that its dosage form is wettable powder, water-dispersed It is any in granula, aqueous emulsion, microemulsion, dispersible oil-suspending agent.
5. the bactericidal composition according to any one of claim 1-4 claims is for preventing answering for corps diseases With.
CN201610636569.6A 2016-08-05 2016-08-05 A kind of bactericidal composition of alkene containing benzo fluorine bacterium azoles and amino-oligosaccharide Active CN106234369B (en)

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CN107018979A (en) * 2017-04-24 2017-08-08 广西南宁益土生物科技有限责任公司 Bactericidal composition containing Chinese yam polysaccharide and benzo alkene fluorine bacterium azoles
CN111436443A (en) * 2020-06-01 2020-07-24 六夫丁作物保护有限公司 Bactericidal disease-resistant composition containing benzovindiflupyr and lentinan

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EP3318129B1 (en) * 2012-12-03 2019-11-06 Bayer CropScience Aktiengesellschaft Method for pest control by applying a combination of paecilomyces lilacinus and fluopyram
CN105409965A (en) * 2014-09-12 2016-03-23 陕西美邦农药有限公司 Sterilization composition containing benzovindiflupyr
CN105432655A (en) * 2014-09-26 2016-03-30 陕西美邦农药有限公司 Bactericidal composition containing benzovindiflupyr
CN104705317A (en) * 2015-02-13 2015-06-17 广西壮族自治区农业科学院农产品质量安全与检测技术研究所 Ultra-low volume liquid containing benzovindiflupyr and triazole fungicide composition

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Effective date of registration: 20180827

Address after: 318050 Shang Jiang Village, Feng Jiang, Luqiao District, Taizhou, Zhejiang (metal profile Park)

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Patentee after: Suzhou yishuimo Biotechnology Co.,Ltd.

Address before: 318050 Shang Jiang Village, Feng Jiang, Luqiao District, Taizhou, Zhejiang (metal profile Park)

Patentee before: Chen Junming