CN104705317A - Ultra-low volume liquid containing benzovindiflupyr and triazole fungicide composition - Google Patents
Ultra-low volume liquid containing benzovindiflupyr and triazole fungicide composition Download PDFInfo
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Abstract
The invention discloses an ultra-low volume liquid containing a benzovindiflupyr and triazole fungicide composition. The ultra-low volume liquid comprises the following components in percentage by weight: 0.1-20% of benzovindiflupyr, 0.1-20% of triazole fungicide, 1-15% of a surface active agent and the balance of a solvent and a cosolvent, wherein the triazole fungicide is any of tebuconazole, difenoconazole epoxiconazole and hexaconazole. The ultra-low volume liquid is suitable for ultra-low volume spraying, low volume spraying and electrostatic spraying, and is used for preventing and curing diseases caused by fungi of paddy rice, corn, wheat, fruit trees, vegetables and tobaccos. The ultra-low volume liquid has the advantages of being simple to process and resistant to rain wash, capable of inhibiting cell sterol demethylation, delaying resistance, enlarging targets and prolonging the lasting period, and saving water and labor.
Description
Technical field
The invention belongs to technical field of pesticide, what be specifically related to is the ultra low volume liquids containing benzo alkene fluorine bacterium azoles and triazole bactericide composition.
Background technology
The shortcomings such as conventional crop controlling mode operating efficiency is low, the large and labour cost of labour intensity, are difficult to adapt to farm and scale scale and plant control requirement to damage by disease and insect.Ultra low volume liquids is the pesticidal preparations being applicable to ultra low volume spraying use, ultra low volume spraying is appearance with ultra low volume sprayer tool and the advanced applications of pesticide new technology of of producing, because of its droplet be fineness uniform oiliness droplet, adhesiveness is strong, the elegant mistake of droplet is little, utilization ratio high, is the first-selected technology of following farm, the plantation of scale scale and public place sterilization etc.20 century 70s take triazolone as the triazole bactericidal agent that representative enters pesticide market, the development prospect of its brilliance is illustrated with its remarkable drug effect, along with the single and frequent use of triazole bactericidal agent in recent years, the resistance of biological target to medicament itself is grown with each passing day.And depend on for existence the explosive disease of the crop such as grain, vegetables, fruit tree and forest bring great threat to human survival and development.
Benzo alkene fluorine bacterium azoles, Chinese: 5-ethyl-6-octyl group [1,2,4] triazol [1,5-A] pyrimidine-7-amine, belonging to pyrazole amide series bactericidal agent, is succinate dehydrogenase inhibitors (SDHIs), with existing methoxy acrylic bactericide and triazole bactericidal agent without cross resistance.It is comprising the outstanding preventive effect many crops such as many crops such as paddy rice, wheat, corn, fruit tree and vegetables all presenting and does harm to main fungal venereal disease.Even if when disease occurs heavier, benzo alkene fluorine bacterium azoles also can provide compared with the existing product preventive effect of long 7 ~ 10 days, and when using as main pesticide control, the comparatively conventional medicament of its performance is more stable.
Tebuconazole, chemical name: (RS)-1-rubigan-4,4-dimethyl-3-(1H-1,2,4-triazol-1-yl methyl) penta-3-alcohol is triazole type high-efficiency broad-spectrum systemic fungicide.Mainly through suppressing the demethylation of sterol in disease fungus body, biomembranous formation is caused to be obstructed and to play bactericidal activity.Have protection, treatment and eradicant action, fungicidal spectrum is extremely wide, activity is high, using dosage is low, the lasting period is long.Can conduct upwards, downwards in plant corpus by plant leaf blade and Root Absorption and be uniformly distributed rapidly after dispenser, not only there is bactericidal activity, also can promote plant growth, improve the immunocompetence of crop.Effectively can prevent and treat the multiple diseases such as the rust of cereal crop, powdery mildew, net blotch, root rot, head blight and smut, be current the most salable triazole type medicament in the world.
Difenoconazole, chemical name: 1-(2-[4-(4-chlorobenzene oxygen)-2-chlorphenyl]-4-methyl isophthalic acid, 3-dioxin-pentane-2-ylmethyl)-1H-1,2,4-triazole (U.S. CA), for triazole type is efficient, wide spectrum, low-toxicity bactericide.By the biosynthesis of T suppression cell wall sterol, stop the growth of fungi, various fungus-caused disease is had to the effect of protection and treatment.Systemic activity is strong, fungicidal spectrum is wide, to ascus subphylum, Basidiomycotina and comprise Alternaria, two armfuls, shell genus, the mould genus of tail spore, genus embraced by thorn dish, ball seat Pseudomonas, Phoma, Ramularia, Septoria, Venturia is interior imperfect fungus, powdery mildew, rest fungus and some kind pass cause of disease mattress lasting protection and therapeutic activity, simultaneously to cercospora leaf spot of sugar beet, wheat glume blotch, leaf blight, rust and the mildew caused by various pathogens, apple scab, powdery mildew, grape powdery mildew, target, leaf spot of peanut, net blotchs etc. all have good preventive effect, and the multiple way property such as leaf surface treatment or seed treatment can be used for.
Epoxiconazole, chemical name: (2RS, 3RS)-1-[3-(2-chlorphenyl)-2,3-epoxy-2-(4-fluorophenyl) propyl group]-1H-1,2,4-triazole, belongs to one of triazole bactericidal agent of novelty.Its active component is by suppressing the synthesis of germ ergosterol, hinder the formation of germ cell wall, and also there is very high inhibition effect to the activity of fungal enzyme (14-dencthylase), compared with bactericide known at present, the generation of the more effective suppression disease fungus of energy.Epoxiconazole can also improve the chitinase activity of crop simultaneously, causes the contraction of fungi haustorium, suppresses germ to invade, and improves crop to the immunocompetence of disease.Epoxiconazole energy effectively preventing Cereal damping off, powdery mildew, eyeprint are sick, leaf spot, powdery mildew, rust on the crop such as banana, green onion garlic, celery, Kidney bean, melon, asparagus, peanut, beet, and the disease such as anthracnose, white rot on grape.
Own azoles alcohol, chemical name: (RS)-2-(2,4-dichlorophenyl)-1-(1H-1,2,4-triazol-1-yl)-own-2-alcohol, belongs to triazole type low-toxicity bactericide.Its mechanism of action is the biosynthesis affecting sterol, and the cell membrane function of pathogen is damaged, and causes cell membrane not formed, and makes germ dead.There is interior suction, protection and therapeutic activity.May be used for apple, grape, banana; vegetables (melon and fruit, capsicum etc.); peanut; coffee; cereal crop and ornamental plants; can effectively prevent and treat disease caused by sac fungi, basidiomycetes and imperfect fungus, especially have excellent protection and eradicant action to the disease that Basidiomycetes and Ascomycetes cause as powdery mildew, rust, scab, brown spot, anthracnose etc., have good preventive effect to rice sheath blight disease.
Benzo alkene fluorine bacterium azoles and existing triazole bactericidal agent are without cross resistance.Triazole bactericidal agent Tebuconazole, Difenoconazole, epoxiconazole, Flusilazole and own azoles alcohol have single dose and the multiple formulations of built agent such as missible oil, microemulsion, aqueous emulsion, suspension seed-coating agent, suspending agent, wetting powder and water dispersible granules common on the market, and also there is not been reported both at home and abroad for the ultra low volume liquids composite with benzo alkene fluorine bacterium azoles.
Summary of the invention
The object of this invention is to provide a kind of ultra low volume liquids containing benzo alkene fluorine bacterium azoles and triazole bactericide composition binary built.
The present invention solves the technical scheme that its technical problem takes:
Containing benzo alkene fluorine bacterium azoles and triazole bactericide composition, it is characterized in that, in described composition, the mass ratio of benzo alkene fluorine bacterium azoles and triazole bactericidal agent is 100:1 ~ 1:100.
Containing the ultra low volume liquids of benzo alkene fluorine bacterium azoles and triazole bactericidal agent, it is characterized in that, described ultra low volume liquids comprises following component by weight percentage:
As preferably, described triazole bactericidal agent is any one in Tebuconazole, Difenoconazole, epoxiconazole and own azoles alcohol.
As preferably, described solvent and cosolvent are selected from acetophenone, phenmethylol, methyl-sulfoxide, 1-METHYLPYRROLIDONE, N, one or more combination in dinethylformamide, DMA, n-octyl alcohol, isoamyl alcohol, terpineol, cyclohexanone, durene, diethylbenzene, adjacent chlorodiphenyl, trimethylbenzene, butylbenzene, methyl naphthalene.
As preferably, described solvent is soybean oil, cottonseed oil, rapeseed oil, castor oil, turpentine oil, esterified vegetable oil as methyl oleate, ethyl oleate, one or more the combination in sulfolane, two wires oil.
As preferably, described surfactant is selected from one or more the combination in fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene, phenethyl phenol polyethenoxy ether, alkylaryl polyoxyethylene polyoxypropylene ether, aliphatic alcohol polyoxyvinethene phosphate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate, alkylaryl polyoxyethylene polyoxypropylene ether phosphate, calcium dodecyl benzene sulfonate.
In addition, present invention also offers the preparation method that described ultra low volume liquids adopts, the steps include: in the mixing kettle of belt stirrer, first with partial solvent by after active component stirring and dissolving, add surfactant and remaining solvent again, fully be uniformly mixed, obtain described ultra low volume liquids; Technological parameter is: it is 60 ~ 150 revs/min that mixing speed controls, and mixing time is 15 ~ 60 minutes, and temperature is normal temperature, and pressure is normal pressure.
The Target crops that described ultra low volume liquids is applicable to is paddy rice (Oryza glaberrima), wheat (Vitaceae), barley (barley), corn (Zea mays), Chinese sorghum (Sorghum), potato (Solanumtuberosum), tomato (Solanum lycopersicum), capsicum (Capsicum annuum Linn), tobacco (Nicotiana tabacum), brassicaceous vegetable and rape (Brassica campestris L.), grape (Vitis vinifera), soybean (Glycine max), peanut (Arachis hypogaea), cucumber (Cucumissativus Linn), lettuce (Lactuca sativa), taro { Colocasia esculenta (L.) Schoot}, watermelon (Citrullus lanatus), longan (Dimocarpus longgana Lour), the crops such as lichee (Litchi chinensis).
Described ultra low volume liquids is suitable for preventing and treating rice blast, bakanae disease, banded sclerotial blight, wheat and barley scab, smut, banded sclerotial blight, rust, powdery mildew, the corn northern leaf blight, helminthosporium maydis, smut, Chinese sorghum smut, leaf spot, cassava brown spot, anthracnose, Cotton blight, anthracnose, verticillium wilt, fusarium wilt, target, gray mold, early blight of tomato, leaf mold, spot blight, anthracnose, pepper anthracnose, early blight etc., Alternaria alternate, frog eye, anthracnose, rape rust, black spot, brown spot, bitter rot or anthracnose of grape, powdery mildew, anthrachose of grape, fruit rot, soybean purple speck, rust, leaf spot of peanut, rust, cucurbits powdery mildew, anthracnose, epidemic disease, fusarium wilt, blight dis-ease, ring rot of apple, powdery mildew, scab, drought period defoliation, anthracnose, ring spot, black rot of pear, scab, mango powdery mildew, anthracnose, peach brown rot, shot hole, leaf-curl, shothole disease, lichee and longan anthracnose, leaf spot, Oidium heveae, anthracnose, eucalyptus gray mold, sigatoka, scab, citrus anthracnose, shot hole, husky skin disease and sugarcane pineapple disease, rust etc.
The method using described ultra low volume liquids is low capacity, ultra low volume or the electrostatic spraying carried out with ground and aircraft, and according to controlling object, a situation arises determines the consumption that is suitable for, and usual ultra low volume spraying formulation rate is 1 ~ 5L/hm
2, low-gallonage spraying formulation rate is 5 ~ 50L/hm
2, electrostatic spray formulation rate is 1 ~ 5L/hm
2.
Compared with prior art, the beneficial effect that the present invention has is:
1, work preventive effect is high.Ultra low volume spraying spraying reaches tens meters, and People's Bank of China's spray velocity kind per second can reach 0.5 ~ 1 meter, and in the unit interval, the area of spray medicine is several times to tens times of conventional insecticide-applying way, and efficiency is high.Secondly, ultra low volume liquids belongs to oily preparation, strong in crop surface adhesiveness, and drug effect is 3-5 times of conventional spraying.
2, increase succinate dehydrogenase suppression, delaying drug resistance, expands target, Synergistic.Triazole bactericidal agent belongs to the biosynthesis inhibitor of sterol, by hindering the biosynthesis of sterol, the cell membrane function of pathogen is damaged.Composite with benzo alkene fluorine bacterium azoles, can effectively increase triazole bactericidal agent and succinate dehydrogenase is suppressed, expand target, delay Disease Resistance and increase, play synergistic function.
3, safety is high.Ultra low volume liquids adopts low toxogen medicine and solvent, low to natural enemy insect, human body and herding toxicity; Residual few in use procedure, few to ecological threat.
Embodiment
Below in conjunction with specific embodiment, elaboration detailed is further done to the present invention, but embodiments of the present invention are not limited to the scope that embodiment represents.
One, indoor joint toxicity measuring experiment
experiment embodiment 1: benzo alkene fluorine bacterium azoles and Tebuconazole mixture toxicity test are tested
For understanding benzo alkene fluorine bacterium azoles and Tebuconazole combine the definite virulence of mixture to Rhizoctonia solani Kuhn, the present invention screens for its proportioning.
Rhizoctonia solani Kuhn (being separated from surrounding area, Nanning), purifying cultivate after through Koch's Postulates verify its pathogenic after by strain inoculation in PDA medium, at 25 DEG C, under 90% damp condition, cultivate 7d, break into bacterium cake with 0.4cm card punch after mycelia covers with culture dish and use.
Mycelial growth rate method is adopted to measure benzo alkene fluorine bacterium azoles, Tebuconazole and the two composition are on the impact of Rhizoctonia solani Kuhn mycelial growth, both settings weight proportion is 100:1, 50:1, 30:1, 15:1, 1:1, 1:15, 1:30, 1:50, 1:100 and each single dose be totally 9 kinds of chemicals treatment, each process setting 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32mg/L totally 9 concentration gradients makes PDA flat board, arranging without medicament is contrast, every concentration repeats 3 times, after aseptically the bacterium cake accomplished fluently being inverted in the dull and stereotyped central authorities of each process PDA, flat board is placed in 25 DEG C, cultivate under 90% damp condition, (about 10d) investigation result when mycelial growth diameter reaches culture dish diameter about 50%, calculate inhibiting rate according to the following equation, and calculate that each chemicals treatment is to virulence regression equation by DPS Software of Data Statistics, correlation coefficient, EC50 and composite co-toxicity coefficient.
Synergy evaluation criterion: co-toxicity coefficient (CTC)≤80 is antagonism, 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect, and CTC >=150 synergy is very remarkable.Result of the test is as shown in table 1:
Table 1 benzo alkene fluorine bacterium azoles and Tebuconazole different ratio are to the toxicity test of Rhizoctonia solani Kuhn
As can be seen from Table 1, when benzo alkene fluorine bacterium azoles and Tebuconazole are in mass ratio between 100:1 ~ 1:100, two end points process CTC values, between 80 to 120, present certain drug effect summation action; When proportioning is between 50:1 ~ 1:50, each process CTC is all greater than 120, and show that synergistic effect is remarkable, especially proportioning is within the scope of 15:1 ~ 1:15, and each process CTC is all greater than 150, shows that synergistic effect is very remarkable.
experiment embodiment 2: benzo alkene fluorine bacterium azoles and Difenoconazole mixture toxicity test are tested
For understanding benzo alkene fluorine bacterium azoles and Difenoconazole combine the definite virulence of mixture to Rhizoctonia solani Kuhn, the present invention screens for its proportioning.
Rhizoctonia solani Kuhn (being separated from surrounding area, Nanning), purifying cultivate after through Koch's Postulates verify its pathogenic after by strain inoculation in PDA medium, at 25 DEG C, under 90% damp condition, cultivate 7d, break into bacterium cake with 0.4cm card punch after mycelia covers with culture dish and use.
Mycelial growth rate method is adopted to measure benzo alkene fluorine bacterium azoles, Difenoconazole and the two composition are on the impact of Rhizoctonia solani Kuhn mycelial growth, both settings weight proportion is 100:1, 50:1, 30:1, 15:1, 1:1, 1:15, 1:30, 1:50, 1:100 and each single dose be totally 9 kinds of chemicals treatment, each process setting 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32mg/L totally 9 concentration gradients makes PDA flat board, arranging without medicament is contrast, every concentration repeats 3 times, after aseptically the bacterium cake accomplished fluently being inverted in the dull and stereotyped central authorities of each process PDA, flat board is placed in 25 DEG C, cultivate under 90% damp condition, (about 10d) investigation result when mycelial growth diameter reaches culture dish diameter about 50%, calculate inhibiting rate according to the following equation, and calculate that each chemicals treatment is to virulence regression equation by DPS Software of Data Statistics, correlation coefficient, EC50 and composite co-toxicity coefficient.
Synergy evaluation criterion: co-toxicity coefficient (CTC)≤80 is antagonism, 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect, and CTC >=150 synergy is very remarkable.Result of the test is as shown in table 2:
Table 2 benzo alkene fluorine bacterium azoles and Difenoconazole different ratio are to the toxicity test of Rhizoctonia solani Kuhn
| Reagent agent | Virulence regression equation | Correlation coefficient | EC50(mg/L) | Co-toxicity coefficient |
| Benzo alkene fluorine bacterium azoles | y＝4.7435+0.2769x | 0.9854 | 1.1278 | - |
| Difenoconazole | y＝2.6437+3.1826x | 0.9426 | 1.7348 | - |
| Benzo alkene fluorine bacterium azoles Difenoconazole (100:1) | y＝1.9729+1.7826x | 0.9978 | 1.1364 | 101.34 |
| Benzo alkene fluorine bacterium azoles Difenoconazole (50:1) | y＝1.7157+0.7243x | 0.9954 | 0.9319 | 125.12 |
| Benzo alkene fluorine bacterium azoles Difenoconazole (30:1) | y＝5.7573+0.7317x | 0.9678 | 0.8926 | 133.54 |
| Benzo alkene fluorine bacterium azoles Difenoconazole (15:1) | y＝2.7286+0.6285x | 0.9986 | 0.6783 | 147.13 |
| Benzo alkene fluorine bacterium azoles Difenoconazole (1:1) | y＝2.2548+2.1989x | 0.9989 | 0.1469 | 169.08 |
| Benzo alkene fluorine bacterium azoles Difenoconazole (1:15) | y＝1.9851+5.4591x | 0.9862 | 0.7526 | 156.37 |
| Benzo alkene fluorine bacterium azoles Difenoconazole (1:30) | y＝0.9767+3.4689x | 0.9526 | 0.9263 | 141.36 |
| Benzo alkene fluorine bacterium azoles Difenoconazole (1:50) | y＝2.3789+1.2518x | 0.9436 | 1.1748 | 129.16 |
| Benzo alkene fluorine bacterium azoles Difenoconazole (1:100) | y＝5.3542+0.9788x | 0.9212 | 1.4879 | 103.68 |
As can be seen from Table 2, when benzo alkene fluorine bacterium azoles and Difenoconazole are in mass ratio between 100:1 ~ 1:100, two end points process CTC values, between 80 to 120, present certain drug effect summation action; When proportioning is between 100:1 ~ 1:100, each process CTC is all greater than 120, shows that synergistic effect is remarkable.
experiment embodiment 3: benzo alkene fluorine bacterium azoles and epoxiconazole mixture toxicity test are tested
For understanding benzo alkene fluorine bacterium azoles and epoxiconazole combine the definite virulence of mixture to Rhizoctonia solani Kuhn, the present invention screens for its proportioning.
Rhizoctonia solani Kuhn (being separated from surrounding area, Nanning), purifying cultivate after through Koch's Postulates verify its pathogenic after by strain inoculation in PDA medium, at 25 DEG C, under 90% damp condition, cultivate 7d, break into bacterium cake with 0.4cm card punch after mycelia covers with culture dish and use.
Mycelial growth rate method is adopted to measure benzo alkene fluorine bacterium azoles, epoxiconazole and the two composition are on the impact of Rhizoctonia solani Kuhn mycelial growth, both settings weight proportion is 100:1, 50:1, 30:1, 15:1, 1:1, 1:15, 1:30, 1:50, 1:100 and each single dose be totally 9 kinds of chemicals treatment, each process setting 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32mg/L totally 9 concentration gradients makes PDA flat board, arranging without medicament is contrast, every concentration repeats 3 times, after aseptically the bacterium cake accomplished fluently being inverted in the dull and stereotyped central authorities of each process PDA, flat board is placed in 25 DEG C, cultivate under 90% damp condition, (about 10d) investigation result when mycelial growth diameter reaches culture dish diameter about 50%, calculate inhibiting rate according to the following equation, and calculate that each chemicals treatment is to virulence regression equation by DPS Software of Data Statistics, correlation coefficient, EC50 and composite co-toxicity coefficient.
Synergy evaluation criterion: co-toxicity coefficient (CTC)≤80 is antagonism, 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect, and CTC >=150 synergy is very remarkable.Result of the test is as shown in table 3:
Table 3 benzo alkene fluorine bacterium azoles and epoxiconazole different ratio are to the toxicity test of Rhizoctonia solani Kuhn
| Reagent agent | Virulence regression equation | Correlation coefficient | EC50(mg/L) | Co-toxicity coefficient |
| Benzo alkene fluorine bacterium azoles | y＝4.7435+0.2769x | 0.9854 | 1.1278 | - |
| Epoxiconazole | y＝2.1426+1.1827x | 0.9764 | 1.8862 | - |
| Benzo alkene fluorine bacterium azoles epoxiconazole (100:1) | y＝1.8726+0.7829x | 0.9938 | 1.1635 | 121.06 |
| Benzo alkene fluorine bacterium azoles epoxiconazole (50:1) | y＝1.7157+0.7243x | 0.9954 | 0.9309 | 124.77 |
| Benzo alkene fluorine bacterium azoles epoxiconazole (30:1) | y＝3.7573+0.7246x | 0.9678 | 0.8746 | 132.66 |
| Benzo alkene fluorine bacterium azoles epoxiconazole (15:1) | y＝2.7145+0.9869x | 0.9986 | 0.6857 | 146.34 |
| Benzo alkene fluorine bacterium azoles epoxiconazole (1:1) | y＝3.2224+3.6575x | 0.9998 | 0.1226 | 165.19 |
| Benzo alkene fluorine bacterium azoles epoxiconazole (1:15) | y＝2.4682+1.5663x | 0.9871 | 0.5736 | 154.33 |
| Benzo alkene fluorine bacterium azoles epoxiconazole (1:30) | y＝1.6767+3.4689x | 0.9976 | 0.8258 | 146.37 |
| Benzo alkene fluorine bacterium azoles epoxiconazole (1:50) | y＝1.6678+6.1687x | 0.9935 | 1.1635 | 128.34 |
| Benzo alkene fluorine bacterium azoles epoxiconazole (1:100) | y＝2.8965+0.6355x | 0.9836 | 1.4679 | 112.67 |
As can be seen from Table 3, when benzo alkene fluorine bacterium azoles and epoxiconazole are in mass ratio between 100:1 ~ 1:100, two end points process CTC values, between 80 to 120, present certain drug effect summation action; When proportioning is between 100:1 ~ 1:100, each process CTC is all greater than 80, and show that synergistic effect is remarkable, especially proportioning is within the scope of 100:1 ~ 1:50, and each process CTC is all greater than 120, shows that synergistic effect is very remarkable.
experiment embodiment 4: benzo alkene fluorine bacterium azoles and own azoles alcohol mixture toxicity test are tested
For understanding benzo alkene fluorine bacterium azoles and own azoles alcohol combine the definite virulence of mixture to Rhizoctonia solani Kuhn, the present invention screens for its proportioning.
Rhizoctonia solani Kuhn (being separated from surrounding area, Nanning), purifying cultivate after through Koch's Postulates verify its pathogenic after by strain inoculation in PDA medium, at 25 DEG C, under 90% damp condition, cultivate 7d, break into bacterium cake with 0.4cm card punch after mycelia covers with culture dish and use.
Mycelial growth rate method is adopted to measure benzo alkene fluorine bacterium azoles, own azoles alcohol and the two composition are on the impact of Rhizoctonia solani Kuhn mycelial growth, both settings weight proportion is 100:1, 50:1, 30:1, 15:1, 1:1, 1:15, 1:30, 1:50, 1:100 and each single dose be totally 9 kinds of chemicals treatment, each process setting 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32mg/L totally 9 concentration gradients makes PDA flat board, arranging without medicament is contrast, every concentration repeats 3 times, after aseptically the bacterium cake accomplished fluently being inverted in the dull and stereotyped central authorities of each process PDA, flat board is placed in 25 DEG C, cultivate under 90% damp condition, (about 10d) investigation result when mycelial growth diameter reaches culture dish diameter about 50%, calculate inhibiting rate according to the following equation, and calculate that each chemicals treatment is to virulence regression equation by DPS Software of Data Statistics, correlation coefficient, EC50 and composite co-toxicity coefficient.
Synergy evaluation criterion: co-toxicity coefficient (CTC)≤80 is antagonism, 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect, and CTC >=150 synergy is very remarkable.Result of the test is as shown in table 4:
Table 4 benzo alkene fluorine bacterium azoles and own azoles alcohol different ratio are to the toxicity test of Rhizoctonia solani Kuhn
| Reagent agent | Virulence regression equation | Correlation coefficient | EC50(mg/L) | Co-toxicity coefficient |
| Benzo alkene fluorine bacterium azoles | y＝4.7435+0.2769x | 0.9854 | 1.1278 | - |
| Own azoles alcohol | y＝2.8526+1.9125x | 0.9757 | 1.8416 | - |
| The own azoles alcohol (100:1) of benzo alkene fluorine bacterium azoles | y＝1.6767+0.7857x | 0.9226 | 0.9686 | 109.35 |
| The own azoles alcohol (50:1) of benzo alkene fluorine bacterium azoles | y＝2.7157+1.7796x | 0.9318 | 0.4378 | 121.07 |
| The own azoles alcohol (30:1) of benzo alkene fluorine bacterium azoles | y＝1.7575+0.8327x | 0.9477 | 0.3655 | 140.21 |
| The own azoles alcohol (15:1) of benzo alkene fluorine bacterium azoles | y＝3.7219+0.9022x | 0.9696 | 0.2467 | 157.10 |
| The own azoles alcohol (1:1) of benzo alkene fluorine bacterium azoles | y＝1.6367+0.7192x | 0.9993 | 0.1489 | 179.18 |
| The own azoles alcohol (1:15) of benzo alkene fluorine bacterium azoles | y＝3.1788+0.8916x | 0.9332 | 0.4786 | 154.59 |
| The own azoles alcohol (1:30) of benzo alkene fluorine bacterium azoles | y＝1.2535+0.6867x | 0.9298 | 0.7659 | 141.82 |
| The own azoles alcohol (1:50) of benzo alkene fluorine bacterium azoles | y＝2.9436+0.6138x | 0.9665 | 1.0256 | 119.27 |
| The own azoles alcohol (1:100) of benzo alkene fluorine bacterium azoles | y＝0.8676+1.4686x | 0.9398 | 1.4892 | 102.67 |
As can be seen from Table 4, when benzo alkene fluorine bacterium azoles and own azoles alcohol are in mass ratio between 100:1 ~ 1:100, two end points process CTC values, between 80 to 120, present certain drug effect summation action; When proportioning is between 50:1 ~ 1:50, each process CTC is all greater than 120, and show that synergistic effect is remarkable, especially proportioning is within the scope of 30:1 ~ 1:30, and each process CTC is all greater than 140, shows that synergistic effect is very remarkable.
Two, embodiment prepared by preparation
embodiment 1: 20.1% benzo alkene fluorine bacterium azoles Tebuconazole ultra low volume liquids
Under normal temperature and pressure, taking the proportioning raw material of recipe requirements, first solvent is dropped in reactor, open reactor agitator, drop into benzo alkene fluorine bacterium azoles and the former medicine of triazole bactericidal agent under agitation, low whipping speed is under 160 ~ 250 revs/min, until completely dissolved (about 15 minutes), drop into cosolvent, auxiliary agent, material adds rear continuation and stirs 15-60 minute, obtains the oily product of homogeneous transparent.
According to the step identical with embodiment 1, following examples can be prepared.
embodiment 2: 20% benzo alkene fluorine bacterium azoles Tebuconazole ultra low volume liquids
embodiment 3: 24% benzo alkene fluorine bacterium azoles Tebuconazole ultra low volume liquids
embodiment 4: 20.1% benzo alkene fluorine bacterium azoles Tebuconazole ultra low volume liquids
embodiment 5: 30.1% benzo alkene fluorine bacterium azoles Difenoconazole ultra low volume liquids
embodiment 6: 30% benzo alkene fluorine bacterium azoles Difenoconazole ultra low volume liquids
embodiment 7: 32% benzo alkene fluorine bacterium azoles Difenoconazole ultra low volume liquids
embodiment 8: 20.1% benzo alkene fluorine bacterium azoles Difenoconazole ultra low volume liquids
embodiment 9: 30.1% benzo alkene fluorine bacterium azoles epoxiconazole ultra low volume liquids
embodiment 10: 33% benzo alkene fluorine bacterium azoles epoxiconazole ultra low volume liquids
embodiment 11: 29% benzo alkene fluorine bacterium azoles epoxiconazole azoles ultra low volume liquids
embodiment 12: 20.1% benzo alkene fluorine bacterium azoles epoxiconazole ultra low volume liquids
embodiment 13: 30.1% benzo alkene fluorine bacterium azoles own azoles alcohol ultra low volume liquids
embodiment 14: 31% benzo alkene fluorine bacterium azoles own azoles alcohol ultra low volume liquids
embodiment 15: 18% benzo alkene fluorine bacterium azoles own azoles alcohol ultra low volume liquids
embodiment 16: 20.1% benzo alkene fluorine bacterium azoles own azoles alcohol ultra low volume liquids
Three, field efficacy embodiment
Advantage containing the ultra low volume liquids of benzo alkene fluorine bacterium azoles and triazole bactericide composition is verified by field control effectiveness test, but is not limited to control crop and the object of test example.
field trial example 1: containing the field trial of the ultra low volume liquids water prevention rice sheath blight of benzo alkene fluorine bacterium azoles and triazole bactericide composition.
The three kinds of mixtures embodiment of the present invention 1 ~ 3 obtained and 5% jinggangmycin A S and 5% Tebuconazole EC carry out field control effectiveness test, if the transplanting paddy field 40m of 1 month is selected in each community
2, repeat 4 times; 14 days investigation preventive effects after medicine, the method for investigation drug effect is: each community diagonal 5 sampling, often some investigation 3 strains, records total strain number and the sick progression of each strain, checks the occurrence degree of rice sheath blight disease.Result of the test is as table 5:
The ultra low volume liquids of table 5 benzo alkene fluorine bacterium azoles and triazole bactericide composition is to the control efficiency of rice sheath blight disease
Field test results shows: benzo alkene fluorine bacterium azoles and the composite ultra low volume liquids of Tebuconazole can the effective water prevention rice sheath blight, and effect is better than the control efficiency of Tebuconazole and jinggangmeisu single dose.
field trial example 2: containing the field trial of the ultra low volume liquids water prevention rice sheath blight of benzo alkene fluorine bacterium azoles and triazole bactericide composition.
The three kinds of mixtures embodiment of the present invention 1 ~ 3 obtained and 5% jinggangmycin A S and 7.5% epoxiconazole EC carry out field control effectiveness test, if the transplanting paddy field 40m of 1 month is selected in each community
2, repeat 4 times; 14 days investigation preventive effects after medicine, the method for investigation drug effect is: each community diagonal 5 sampling, often some investigation 3 strains, records total strain number and the sick progression of each strain, checks the occurrence degree of rice sheath blight disease.Result of the test is as table 6:
The ultra low volume liquids of table 6 benzo alkene fluorine bacterium azoles and triazole bactericide composition is to the control efficiency of rice sheath blight disease
Field test results shows: benzo alkene fluorine bacterium azoles and the composite ultra low volume liquids of Tebuconazole can the effective water prevention rice sheath blight, and effect is better than the control efficiency of epoxiconazole and jinggangmeisu single dose.
field trial example 3: the field trial of the ultra low volume liquids control of leaf spot of corn of benzo alkene fluorine bacterium azoles and triazole bactericidal agent.
The mixture embodiment of the present invention obtained and triazole bactericidal agent conventional dose carry out field control effectiveness test, if each plot area is corn field area 60m
2, repeat 3 times; 7 days, 14 days investigation preventive effects after medicine, the method for investigation drug effect is: 5 pockets are investigated in each community, and every pocket gets a point by east, south, west, north, middle direction, and often some investigation 2 strains, record total strain number and diseased plant number at different levels, check the occurrence degree of helminthosporium maydis.Result of the test is as table 7:
The ultra low volume liquids of table 7 benzo alkene fluorine bacterium azoles and triazole bactericide composition is to the control efficiency of corn southern leaf blight
Field test results shows: the ultra low volume liquids containing benzo alkene fluorine bacterium azoles and triazole bactericidal agent can effective control of leaf spot of corn, and lasting effect is better than the control efficiency of conventional benzo alkene fluorine bacterium azoles single dose and triazole bactericidal agent single dose.Show, the preventive effect of ultra low volume liquids is better than missible oil, water dispersible granules and the suspending agent control efficiency to corn southern leaf blight simultaneously.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.
Claims (7)
1. containing benzo alkene fluorine bacterium azoles and triazole bactericide composition, it is characterized in that, in described composition, the mass ratio of benzo alkene fluorine bacterium azoles and triazole bactericidal agent is 100:1 ~ 1:100, and described triazole bactericidal agent is any one in Tebuconazole, Difenoconazole, epoxiconazole and own azoles alcohol.
2. contain the ultra low volume liquids of benzo alkene fluorine bacterium azoles and triazole bactericide composition, it is characterized in that,
Described ultra low volume liquids comprises following component by weight percentage:
3. ultra low volume liquids according to claim 2, is characterized in that, described triazole bactericidal agent is any one in Tebuconazole, Difenoconazole, epoxiconazole and own azoles alcohol.
4. ultra low volume liquids according to claim 2, it is characterized in that, described surfactant is the combination of one or more in benzylbiphenyl phenol polyethenoxy ether, polyoxyethylene nonylphenol ether, fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene, phenethyl phenol polyethenoxy ether, alkylaryl polyoxyethylene polyoxypropylene ether, aliphatic alcohol polyoxyvinethene phosphate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate, alkylaryl polyoxyethylene polyoxypropylene ether phosphate, calcium dodecyl benzene sulfonate.
5. ultra low volume liquids according to claim 2, is characterized in that, described solvent is one or more combinations in aromatic hydrocarbon solvent and polar solvent; Described aromatic hydrocarbon solvent comprises dimethylbenzene, trimethylbenzene, durene, diethylbenzene, adjacent chlorodiphenyl, butylbenzene; Described polar solvent comprises phenmethylol, 1-METHYLPYRROLIDONE, DMF, DMA, n-hexyl alcohol, n-octyl alcohol, terpineol, cyclohexanone.
6. ultra low volume liquids according to claim 2, is characterized in that, described cosolvent is one or more combinations in two wires oil, soybean oil, cottonseed oil, castor oil, corn oil, turpentine oil, methyl oleate, ethyl oleate, sulfolane.
7. according to the arbitrary described ultra low volume liquids of claim 2-6, it is characterized in that, the preparation method of described ultra low volume liquids is: in the mixing kettle of belt stirrer, first with partial solvent by after active component stirring and dissolving, add surfactant and remaining solvent again, fully be uniformly mixed, obtain the ultra low volume liquids containing benzo alkene fluorine bacterium azoles and triazole bactericide composition; Technological parameter is: it is 60 ~ 150 revs/min that mixing speed controls, and mixing time is 15 ~ 60 minutes, and temperature is normal temperature, and pressure is normal pressure.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234369A (en) * | 2016-08-05 | 2016-12-21 | 李祥英 | A kind of containing benzo alkene fluorine bacterium azoles and the bactericidal composition of amino-oligosaccharide |
| CN106234370A (en) * | 2016-08-23 | 2016-12-21 | 李祥英 | A kind of containing benzo alkene fluorine bacterium azoles and the bactericidal composition of osthole |
| WO2018236716A1 (en) * | 2017-06-19 | 2018-12-27 | Agrinos AS | Compositions and methods for increasing crop yield |
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| CN101232814A (en) * | 2005-07-27 | 2008-07-30 | 巴斯福股份公司 | Fungicidal mixtures based on azolopyrimidinylamines |
| CN103688955A (en) * | 2013-12-13 | 2014-04-02 | 广西田园生化股份有限公司 | Ultra-low volume liquid containing ametoctradin and triazole bactericide composition as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101232814A (en) * | 2005-07-27 | 2008-07-30 | 巴斯福股份公司 | Fungicidal mixtures based on azolopyrimidinylamines |
| CN103688955A (en) * | 2013-12-13 | 2014-04-02 | 广西田园生化股份有限公司 | Ultra-low volume liquid containing ametoctradin and triazole bactericide composition as well as preparation method and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106234369A (en) * | 2016-08-05 | 2016-12-21 | 李祥英 | A kind of containing benzo alkene fluorine bacterium azoles and the bactericidal composition of amino-oligosaccharide |
| CN106234370A (en) * | 2016-08-23 | 2016-12-21 | 李祥英 | A kind of containing benzo alkene fluorine bacterium azoles and the bactericidal composition of osthole |
| WO2018236716A1 (en) * | 2017-06-19 | 2018-12-27 | Agrinos AS | Compositions and methods for increasing crop yield |
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