A kind of containing benzo alkene fluorine bacterium azoles and the bactericidal composition of amino-oligosaccharide
Technical field
The present invention relates to composition pesticide field, particularly relate to a kind of containing benzo alkene fluorine bacterium azoles and amino-oligosaccharide
Bactericidal composition.
Summary of the invention
Powdery mildew is the worldwide disease of a kind of extensive generation, main employing chemical pesticide control powdery mildew, mesh in production
Before rely primarily on benzimidazole, triazole type, methoxy acrylic bactericide.But, killing of life-time service single kind
Microbial inoculum can make disease produce resistance in various degree, and prevention effect reduces.
Benzo alkene fluorine bacterium azoles is a kind of pyrazole amide series bactericidal agent, by electron transport chain on suppression mitochondrial inner membrane
Succinate dehydrogenase so that pathogenic fungi cannot produce energy via breathing, and then disintegrates the growth of pathogenic bacteria, and this compound is independent
Use cost is higher and easily sends out again the generation of resistance, thus reduces the service life of pesticide species.
Amino-oligosaccharide (oligochitosan) refers to the oligosaccharide that D-glucosamine connects with β-1.4 glycosidic bond, by chitin
Say that after solving chitosan, explanation prepares again, or the low toxicity antibacterial extracted by fermentable.Amino-oligosaccharide can be sick to some
The growth of bacterium produces inhibitory action, affect the sprouting of fungal spore, induces that hypha form morphs, biochemistry changes in spore
Deng.Gene in energy activated plant body, produces and has the chitinase of resistant effect, glucanase, guarantor's element and PR albumen etc., and have
Cell activation effect, the recovery of the plant that contributes to being injured, promote root strong sprout, to strengthen the resistance of crop, promote growth and development of plants.
Amino-oligosacchride cellulose solution has virus killing, bactericidal, fungicidal action.Not only antibacterial, fungus, virus are had extremely strong preventing and treating and
Eradicant action, but also have nutrition, regulate, detoxify, antibacterial effect.Not only fungus, antibacterial, virus had extremely strong preventing
Control and eradicant action, but also have nutrition, regulate, detoxify, antibacterial effect.Can be widely used for preventing and treating fruit tree, vegetable and cotton
The diseases such as mosaic disease that grain crop causes, powdery mildew, little leaf, spot disease, anthrax, downy mildew, epidemic disease.
The recipe determination of benzo alkene fluorine bacterium azoles and amino-oligosaccharide is conducted in-depth research by present inventor, finds
Benzo alkene fluorine bacterium azoles and amino-oligosaccharide carry out mixture and have obvious Synergistic work in certain scope to multiple fungus
With, complete the present invention after further study.
There is no the relevant report of the complex composition of benzo alkene fluorine bacterium azoles and amino-oligosaccharide at present.
Summary of the invention
It is an object of the invention to, it is provided that a kind of Synergistic bactericidal composition being suitable for agriculturally using, contribute to reducing and use
Dose, the generation delaying pathogen resistance and reduction use cost.
For solving above-mentioned technical problem, the present invention adopts the following technical scheme that a kind of Synergistic bactericidal composition, and its feature exists
In, active component contains benzo alkene fluorine bacterium azoles and amino-oligosaccharide, wherein, benzo alkene fluorine bacterium azoles and the mass ratio of amino-oligosaccharide
For 1:40-40:1, preferably 1:10-10:1.
Further, in described bactericidal composition, the weight ratio of active component is 1-90%, preferably 10-70%.
Further, described bactericidal composition can be processed into agricultural by preparation method conventional in Pesticide formulation
Any dosage form of upper permission, such as, can be wettable powder, water dispersible granules, aqueous emulsion, microemulsion.
The compositions of the present invention can be the most blended with each material in the finished product preparation i.e. compositions of form offer, it is also possible to
Unit dose form provides, and is directly mixed in proportion in bucket or tank, is then diluted to required concentration before using.
Further, the compositions of the present invention can also add other agricultural active composition, such as antibacterial, insecticide,
The agricultural active composition such as herbicide, fertilizer.
The bactericidal composition of the present invention is applicable to prevent and kill off the multiple diseases such as powdery mildew of cucumber, Fructus Lycopersici esculenti powdery mildew, powdery mildew of strawberry
Evil.
Compared with prior art, the having the beneficial effect that of the composition pesticide of the present invention
(1), after the compositions of the present invention is compounding, there is in certain scope obvious synergistic function, preventive effect and single work
Property composition is compared and is significantly improved;
(2) in the compositions of the present invention, the active component mechanism of action is different, while effectively preventing and treating crop pest, permissible
Delay the drug-fast generation of pathogen, be conducive to delaying the service life of pesticide activity component;
(3) the compositions preparation technology of the present invention is simple, is suitable for popularization and application in actual production.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, present invention specific examples below
Illustrate, but the present invention is limited to absolutely not these examples.The following stated is only the preferable embodiment of the present invention, is used only for explaining
The present invention, but can not be therefore understands that be the restriction to the scope of the claims of the present invention.It should be pointed out that, all spirit in the present invention and
Any amendment, equivalent or the improvement etc. made within principle, should be included within the scope of the present invention, therefore,
The protection domain of patent of the present invention should be as the criterion with claims.
One, example of formulations
1, embodiment 1:41% benzo alkene fluorine bacterium azoles amino-oligosaccharide water dispersible granules (40:1)
Benzo alkene fluorine bacterium azoles 40%
Amino-oligosaccharide 1%
Sodium lignin sulfonate 4%
Sodium lauryl sulphate 6%
Polyvinyl alcohol 2%
White carbon 1%
Kieselguhr surplus
2, embodiment 2:21% benzo alkene fluorine bacterium azoles amino-oligosaccharide wettable powder (20:1)
Benzo alkene fluorine bacterium azoles 20%
Amino-oligosaccharide 1%
Sodium lauryl sulphate 3%
Sodium lignin sulfonate 2%
Pull open powder 3%
Kieselguhr surplus
3, embodiment 3:25% benzo alkene fluorine bacterium azoles amino-oligosaccharide suspending agent (23:2)
Benzo alkene fluorine bacterium azoles 23%
Amino-oligosaccharide 2%
Sodium lignin sulfonate 8%
Octyl phenol polyglycol ether 3%
Xanthan gum 1%
Glycerol 6%
Deionized water surplus
4, embodiment 4:10% benzo alkene fluorine bacterium azoles amino-oligosaccharide aqueous emulsion (1:1)
Benzo alkene fluorine bacterium azoles 5%
Amino-oligosaccharide 5%
Sodium lauryl sulphate 3%
N-Methyl pyrrolidone 7%
Isobutanol 9%
Polyoxyethylene fatty alcohol 2%
Alkyl polyglycol ether 6%
Deionized water surplus
Two, Toxicity Determination
Subjects: cucumber powdery mildew's pathogen
Test method: test is with reference to People's Republic of China's industry standard NY/T 1156.11-2008 " farm-chemical indoor determination
Test rule antibacterial the 11st part: preventing and treating cucurbits powdery mildew test pot-culture method ", use pot-culture method to measure.
Sowing susceptible cucumber variety, potted plant to grow to 2 leaf periods standby.By the benzo alkene fluorine bacterium azoles prepared and amino
Oligosaccharide mother solution dissolves with 0.1% tween 80 aqueous surfactant solution and prepares Concentraton gradient.And set containing only solvent and surface
Activating agent and make blank without the process of effective ingredient.24 h after chemicals treatment, spray inoculation cucumber powdery mildew's pathogen spore
Suspension.The process for preparation of spore suspension is as follows: takes and covers with Powdery Mildew blade, with refueling a small amount of tween 80 surface activity
Spore is washed down by the sterilized water of agent, and double gauze filters, and is modulated into 1 × 10 after microscopy5The suspension of individual/mL.Inoculation is placed on
In thermostatic chamber, cultivate 7 days under the conditions of temperature 24 DEG C.When blank disease liquid rate reaches more than 80%, each process is investigated in classification
Incidence, calculates disease index and the prevention effect of each process.
Calculate the potentiation situation of two kinds of medicaments according to colby formula, result of the test is as shown in table 1.
Toxicity Determination result shows, benzo alkene fluorine bacterium azoles and amino-oligosaccharide weight ratio are in the range of 1:40-40:1, right
The co-toxicity coefficient of powdery mildew of cucumber preventing and treating is all higher than 120, shows synergistic function, and especially the weight ratio of compositions is 1:
During 10-10:1, co-toxicity coefficient is all higher than 450, and potentiation becomes apparent from.
Three, field control effectiveness test
In order to verify the practical application effect of the present invention further, the medicament of the embodiment of the present invention is used to carry out preventing and treating Fructus Cucumidis sativi white lead
Sick field control effectiveness test.
Reagent agent: (1) 41% benzo alkene fluorine bacterium azoles amino-oligosaccharide water dispersible granules (40:1);(2) 21% benzo alkene
Fluorine bacterium azoles amino-oligosaccharide wettable powder (20:1);(3) 25% benzo alkene fluorine bacterium azoles amino-oligosaccharide suspending agents (23:
2);(3) 10% benzos alkene fluorine bacterium azoles amino-oligosaccharide aqueous emulsion (1:1).
Within 5 days, 10 days after medicine, investigating disease indexs and calculate preventive effect, result of the test is shown in Table 2.
Field control effectiveness test result shows, powdery mildew of cucumber is all shown relatively by the complex composition preparation of embodiment 1-4
Good prevention effect, in the case of dosage is relatively low, preventive effect is still better than the preventive effect of single active component.It addition, in medication
In the range of, the compositions of the present invention does not finds crop is produced poisoning, and compared with single dose, cucumber growth is more healthy and strong, is worth
Popularization and application in production.