CN109566660A - A kind of application of Lavender - Google Patents
A kind of application of Lavender Download PDFInfo
- Publication number
- CN109566660A CN109566660A CN201910086751.2A CN201910086751A CN109566660A CN 109566660 A CN109566660 A CN 109566660A CN 201910086751 A CN201910086751 A CN 201910086751A CN 109566660 A CN109566660 A CN 109566660A
- Authority
- CN
- China
- Prior art keywords
- lavender
- phytophthora nicotianae
- prevention
- essential oil
- mycelia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
Abstract
The invention discloses a kind of applications of Lavender.The present invention is to realize the green prevention and control of crop disease, and efficient fungicide active ingredient is screened from fragrant plant source, determines Lavender to the bacteriostatic activity of Phytophthora nicotianae using mycelia growth method.The result shows that Lavender has significant inhibiting effect to Phytophthora nicotianae when essential oil concentration reaches 250 μ L/L.Dose-effect experiment the result shows that, under stifling mode, 255.20 μ g/mL of EC50 value that Lavender inhibits Phytophthora nicotianae.Inserted sheet method the experimental results showed that, Lavender has a certain impact to Phytophthora nicotianae mycelia growthform.Many advantages, such as Lavender of the invention has environmental protection, low cytotoxicity, broad spectrum activity and bacterial strain drug resistance is avoided to generate is expected to become the environmentally friendly new pesticide of prevention and treatment Phytophthora nicotianae.
Description
Technical field
The invention belongs to biological pesticide technical fields, and in particular to a kind of application of Lavender.
Background technique
Phytophthora nicotianae (Phytophtora parasitica var. nicotianaeIt Tucker is) that a kind of soil-borne disease is former
Bacterium has extensive host range, worldwide seriously endangers tobacco and other solanaceous crops, especially in Yunnan Tobacco Planting Area,
Serious economic loss is brought to tobacco grower.Phytophthora nicotianae can cause that root, stem rot are rotten and blade is withered, in each of tobacco growing
Breeding time, which can infect, to cause to fall ill.Continuous, a large amount of methods using chemical pesticide are mainly taken to the prevention and treatment of the disease at present, by
This leads to the generation of pathogen Resistance Physiology microspecies, the destruction of soil microbial community, the destruction of farmland ecological environment and agriculture
Product excessive pesticide residues endanger the health care belt of people.Therefore, the biogenic pesticide of research and development safely, effectively, environmentally friendly
It is particularly important to substitute chemical pesticide.
Plants essential oil is derived from the secondary metabolite of plant different parts.In recent years, with to green safe agricultural production
The increase of product demand, appliable plant essential oil become the hot spot of research agricultural disease as the substitute of traditional chemical pesticide.Plant
Essential oil obtains certain progress in antibacterium, the antimycotic and fields such as antiviral, but there is not yet about fragrant plant is utilized
The report of Lavender prevention and treatment Phytophthora nicotianae.
Summary of the invention
The purpose of the present invention is to provide a kind of applications of Lavender.
The object of the present invention is achieved like this, and the Lavender is in preparation prevention and treatment Phytophthora nicotianae fungicide
Using.
The present invention is to realize the green prevention and control of crop disease, and efficient fungicide active ingredient is screened from fragrant plant source,
Lavender is determined to the bacteriostatic activity of Phytophthora nicotianae using mycelia growth method.The result shows that when essential oil concentration reaches 250
When μ L/L, Lavender has significant inhibiting effect to Phytophthora nicotianae.Dose-effect experiment the result shows that, in stifling mode
Under, 255.20 μ g/mL of EC50 value that Lavender inhibits Phytophthora nicotianae.Inserted sheet method the experimental results showed that, Lavender
It has a certain impact to Phytophthora nicotianae mycelia growthform.The present invention is the result shows that Lavender is expected to become prevention and treatment tobacco epidemic disease
Mould environmentally friendly fungicide.
Detailed description of the invention
Fig. 1 is influence of the Lavender to Phytophthora nicotianae bacterium colony size；
Fig. 2 is inhibition efficiency of the Lavender to Phytophthora nicotianae of various concentration；
Fig. 3 is influence of the Lavender to Phytophthora nicotianae hypha form.
Specific embodiment
Below with reference to embodiment and attached drawing, the present invention is further illustrated, but is not subject in any way to the present invention
Limitation, based on present invention teach that it is made it is any transform or replace, all belong to the scope of protection of the present invention.
The application of Lavender of the present invention is the Lavender in preparation prevention and treatment Phytophthora nicotianae sterilization
Application in agent.
The Lavender concentration is 250 μ L/L or more.
Prevention and treatment Phytophthora nicotianae fungicide of the present invention is made of Lavender and organic solvent.
The volume proportion of the Lavender and organic solvent is 1:(1~10).
The organic solvent is Tween-20, acetone or alcohol.
Lavender of the present invention includes the preparation of essential oil agent when preparation prevents and treats Phytophthora nicotianae fungicide, smokes
The use of clothing herbal medicine agent and the observation of Lavender medicament disease-resistant form, specifically include:
1, Lavender medicament is prepared: by Lavender according to the ratio and Tween-20 or acetone or alcohol of 1:1~10 times
Mixing, after mixing well, for use；
2, culture medium essential oil agent suffocating treatment
It is the culture that the Phytophthora nicotianae bacteria cake of 5 mm accesses 20 mL PDA culture mediums that diameter is aseptically taken with punch
Ware (90 mm of diameter) center.Plate is inverted, taking 1 diameter is that the aseptic filter paper of 5 mm is placed in ware lid center, is inhaled respectively
The distinct fragrance plants essential oil of 1,2,3,4 and 5 μ L is taken to drip on filter paper, not add plants essential oil on filter paper
Plate is control.Sealed membrane sealing cultivates 7 d or so at a temperature of being placed on 28 DEG C, measures colony diameter and calculates bacteriostasis rate.
3, regression equation and EC50 measurement
For above 5 kinds of essential oils, it is diluted to the culture medium containing essential oil of series of concentrations gradient respectively.Not homogeneity is measured according to the above method
Concentration essential oil is measured to the bacteriostatic activity of Phytophthora nicotianae, and seeks its regression equation y=a+bx and median effective concentration (EC50
Value).
4, the influence that Lavender medicament grows hypha form
Hypha form is observed using inserted sheet method.Bacteria cake after 3 d of culture is seeded in PDA culture medium respectively, to be free of essential oil
It is control.Colony diameter to be compareed reaches 2 cm or so, and being inserted into 4 coverslips in bacterium colony surrounding, (coverslip need to be soaked in ethyl alcohol
Used after calcination or sterilizing on alcolhol burner flame after bubble), to coverslip be removed, microscope on mycelia " climbing " to coverslip
Lower observation hypha form.
Case is embodied, the present invention will be further described below:
Embodiment 1
Lavender medicament is prepared: Lavender being mixed according to the ratio of 1:1~10 times with Tween-20, is mixed well
Afterwards, then with distilled water it dilutes, makes the final concentration of 100 μ g/mL of Lavender；
Embodiment 2
Lavender medicament is prepared: Lavender mixed according to the ratio of 1:1~10 times with acetone, after mixing well,
It is diluted again with distilled water, makes the final concentration of 200 μ g/mL of Lavender；
Embodiment 3
Lavender medicament is prepared: Lavender mixed according to the ratio of 1:1~10 times with ethyl alcohol, after mixing well,
It is diluted again with distilled water, makes the final concentration of 300 μ g/mL of Lavender；
Embodiment 4
The Phytophthora nicotianae bacterial plaque of essential oil agent suffocating treatment is observed: it is the cigarette of 5 mm that diameter is aseptically taken with punch
Careless phytophthora bacteria cake accesses culture dish (90 mm of the diameter) center of 20 mL PDA culture mediums.Plate is inverted, 1 diameter is taken
It is placed in ware lid center for the aseptic filter paper of 5 mm, draws 5 μ L Lavenders drop on filter paper, not add on filter paper
Add the plate of essential oil for control.7 d or so are cultivated in sealed membrane sealing at a temperature of being placed on 28 DEG C, observation Phytophthora nicotianae bacterial plaque is big
Small and shape.
As a result Lavender has certain In-vitro Inhibitory Effect to Phytophthora nicotianae as shown in Figure 1:, limits Phytophthora nicotianae
The growth of mycelia.Compared with blank control, Lavender processing causes Phytophthora nicotianae bacterium colony significantly to become smaller.
Embodiment 5
Essential oil agent suffocating treatment calculates the inhibiting rate of Phytophthora nicotianae: it is 5 mm that diameter is aseptically taken with punch
Phytophthora nicotianae bacteria cake access 20 mL PDA culture mediums culture dish (90 mm of diameter) center.Plate is inverted, takes 1
The aseptic filter paper that diameter is 5 mm is placed in ware lid center, draws the distinct fragrance plant essence of 1,2,3,4 and 5 μ L respectively
Oil droplet is compareed on filter paper with not adding the plate of plants essential oil on filter paper.Sealed membrane sealing is placed on 28 DEG C of temperature
7 d of lower culture or so measure colony diameter and calculate bacteriostasis rate, 3 repetitions of every processing.Bacteriostasis rate is calculated as follows:
Bacteriostasis rate (%)=(dC-dT)/(dC -5) × 100.In formula: dC is control colony diameter；DT is different disposal
Under the conditions of colony diameter.
As a result as shown in Fig. 2, by calculating the discovery of bacterium colony growth inhibition ratio, Lavender has significantly Phytophthora nicotianae
Inhibitory effect, and there is concentration dependent, with the increase of concentration, inhibit efficiency to increase, at higher concentration (5 μ L)
When, the inhibition efficiency of Lavender medicament has reached 41.5%.
Embodiment 6
Regression equation and EC50 measurement: for the above Lavender, it is diluted to the culture containing essential oil of series of concentrations gradient respectively
Base.Different quality concentration essential oil is measured to the bacteriostatic activity of Phytophthora nicotianae, and ask its regression equation y=a+bx and effectively in
Concentration (EC50 value).
As a result (table 1) is shown, in fumigating system method, Lavender is 255.20 μ g/ to the EC50 value of Phytophthora nicotianae
ML shows that it is preferable to the inhibitory effect of Phytophthora nicotianae.
The regression equation and EC50 value that 1 Lavender of table inhibits Phytophthora nicotianae
| Essential oil | Regression equation | R² | EC50 (μ g/mL) |
| Lavender oil | y = 6.9264x + 9.6162 | 0.95 | 255.20 |
Embodiment 7
The influence that Lavender medicament grows hypha form: hypha form is observed using inserted sheet method.By the bacterium after 3 d of culture
Cake is seeded in PDA culture medium, to be control without essential oil.Colony diameter to be compareed reaches 2 cm or so, in bacterium colony surrounding
It is inserted into 4 coverslips (coverslip need to use after calcination or sterilizing on alcolhol burner flame after ethyl alcohol immersion), " is climbed " to mycelia
To coverslip, coverslip is removed, microscopically observation hypha form.
As a result as Fig. 3 shows that Lavender has large effect to Phytophthora nicotianae mycelia growth conditions.Blank control group
Hypha form at uniform filiform, inner material is evenly distributed, and Hyphal length is longer, and bifurcated is less, without apparent different
Often expand；The Phytophthora nicotianae hyphal surface handled by Lavender is no longer smooth, and mycelia bending increases, and sends out on mycelia
Bud increases, and mycelia branch increased significantly.Part mycelia tubercle expands, and vacuole occurs in inside, it may be possible at Lavender
Hyphal cell internal structure is destroyed after reason, damaged membrane, and Dissolve things inside is discharged into extracellular caused.
Claims (5)
1. a kind of application of Lavender, it is characterised in that the Lavender prevents and treats Phytophthora nicotianae fungicide in preparation
In application.
2. the application of Lavender according to claim 1, it is characterised in that the Lavender concentration is 250
μ L/L or more.
3. a kind of prevention and treatment Phytophthora nicotianae fungicide, it is characterised in that the prevention and treatment Phytophthora nicotianae fungicide by Lavender and
Organic solvent composition.
4. prevention and treatment Phytophthora nicotianae fungicide according to claim 3, it is characterised in that the Lavender and organic
The volume proportion of solvent is 1:(1~10).
5. prevention and treatment Phytophthora nicotianae fungicide according to claim 3 or 4, it is characterised in that the organic solvent is to spit
Warm -20, acetone or alcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910086751.2A CN109566660A (en) | 2019-01-29 | 2019-01-29 | A kind of application of Lavender |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910086751.2A CN109566660A (en) | 2019-01-29 | 2019-01-29 | A kind of application of Lavender |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109566660A true CN109566660A (en) | 2019-04-05 |
Family
ID=65918079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910086751.2A Pending CN109566660A (en) | 2019-01-29 | 2019-01-29 | A kind of application of Lavender |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109566660A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113265442A (en) * | 2021-05-21 | 2021-08-17 | 上海会佳果蔬专业合作社 | Evaluation method for components and effects of lavender flowers |
-
2019
- 2019-01-29 CN CN201910086751.2A patent/CN109566660A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| F. DIANEZ 等: "Screening of antifungal activity of 12 essential oils against eight pathogenic fungi of vegetables and mushroom", 《LETTERS IN APPLIED MICROBIOLOGY》 * |
| 郑小波: "《疫霉菌及其研究技术》", 31 March 1997, 中国农业出版社，第1版 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113265442A (en) * | 2021-05-21 | 2021-08-17 | 上海会佳果蔬专业合作社 | Evaluation method for components and effects of lavender flowers |
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Application publication date: 20190405 |