CN107232238B - A kind of application of litsea cubeba oil on control capsicum epidemic disease - Google Patents
A kind of application of litsea cubeba oil on control capsicum epidemic disease Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
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Abstract
The invention discloses a kind of application of litsea cubeba oil on control capsicum epidemic disease, and litsea cubeba oil is dissolved in organic solvent, adds emulsifying agent Tween, finally with sterile water dilution, mixing, obtain the medical fluid of required litsea cubeba oil concentration；Medical fluid is administered by the way of spraying or pouring root；Wherein, the litsea cubeba oil is prepared by the following steps: by the drying of fruit of a cubeb litsea tree fruit, being ground up, sieved, is obtained fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil.It is a kind of inhibitor of effective phytophthora blight of pepper using medical fluid made of litsea cubeba oil of the present invention, there is good field control effect to capsicum epidemic disease, can be used for the comprehensive treatment of capsicum epidemic disease.Disease occur different phase it can be used to be prevented and treated, especially sporangiform at apply before such medicament can be effectively suppressed pathogen infect again and the developmenting spread of disease.
Description
Technical field
The invention belongs to cultural control technical fields, and in particular to a kind of litsea cubeba oil answering on control capsicum epidemic disease
With.
Background technique
Litsea cubeba oil is extracted from Lauraceae Litsea machaka fruit of a cubeb litsea tree Litsea cubeba Pers fresh fruit
Volatile oil, be GB2760-2011 be defined as allowing using edible natural fragrance, be that single variety export volume in China's is maximum
Plants essential oil." dictionary of medicinal plant " record its has expelling wind and clearing away cold, qi-regulating desinsection, removing toxic substances analgesic the benefits of, be civil common medicine it
One.Initially, week brave wait finds that litsea cubeba oil is a kind of broad-spectrum antifungal drug by experimental study, and confirms that it has sterilization to make
With.Then, increasing to the research of litsea citrate oil antibacterial action and mechanism, but it is concentrated mainly on food and medical domain.It is remaining
Uncle is good to be waited research shows that litsea cubeba oil is to aspergillus flavus (Aspergillus flauus) common in food, Penicillium citrinum
(Penicillium citrinum), Mucor racemosus (Mucor racemosus) and bread mold (Rhizopus nigricans)
There is stronger antibacterial action etc. a variety of moulds, can be used as the food such as peanut, the corn of mould easy to pollute and aflatoxin
Good natural antisepsis fresh-keeping agent.Litsea cubeba oil is to candida albicans (Candida albicans), trichophyta (Trichophyton
Rubrum), more than ten fungies and the staphylococcus aureus such as aspergillus fumigatus (Aspergillus fumigatus)
(Staphyloccocus aureus), gonococcus (Neisseria gonorrhoeae) and Escherichia coli (Escherichia
) etc. coli more than ten pathogenic bacterias have inhibits and killing effect well, is most wide and forceful drug effect the day of known antimicrobial spectrum
One of right plant extracts.
Currently, less in relation to research report of the litsea cubeba oil to plant pathogenic fungi bioactivity.A variety of millet figured woven silk material age etc. grinds
Studying carefully discovery litsea citrate oil has very strong inhibition plant pathogenic fungi Fusarium oxysporum(Fusarium oxysporum),Helminthosporium sp. , StemphyilliumSp. the ability grown, and have apparent preservation；Yuan Ping etc.
Report that litsea cubeba oil has stronger inhibitory activity to mould.
By phytophthora blight of pepper (Phytophthora capsici) caused by plant disease to have become vegetables all over the world raw
The main restricting factor of production.The germ endangers the various vegetables of Solanaceae, Curcurbitaceae and pulse family and other plant amounts to more than 50 kinds, can
The each position of plant is infected, causes plant withered, root-rot, neckrot, the symptoms such as seedling is dampinged off, leaf is withered, stem is withered, fruit is rotten.By capsicum
Capsicum epidemic disease caused by phytophthora infects has become a kind of crushing soil facsimile disease at present, there is generation all over the world,
Lighter field diseased plant rate of falling ill is 15 ~ 30%, and causing production loss is 20 ~ 25%, and serious field loss is 50% or more, very
To total crop failure.There is the report that capsicum epidemic disease seriously occurs in China pepper planting area such as Sichuan, Guizhou, Shaanxi, Gansu, Guangdong etc.
Road, and with the increase of protecting field pepper planting area, capsicum epidemic disease has the trend aggravated year by year.In recent years, it is
Reduce capsicum epidemic disease caused by lose, both at home and abroad genetic breeding, tillage control measure, in terms of capsicum epidemic disease is prevented
It controls and has conducted extensive research, but do not find Resistant gerplasm material at present；The Agricultural management system for preventing and treating capsicum epidemic disease is more complex,
Large area production cultivation is difficult to carry out；It is still using fungicide that the most common measure of capsicum epidemic disease is prevented and treated in production.20th century 80
Begin to use the prevention and treatment such as systemic fungicide metalaxyl, Sandofan M8, cyazofamid capsicum epidemic disease effect preferable after age, but with
The long-time service of these medicaments has caused the generation of germ drug resistance, and a large amount of uses easily cause environmental pollution, threaten food peace
Entirely, it causes damages to human health.
Summary of the invention
The object of the present invention is to provide application of the litsea cubeba oil on control crop capsicum epidemic disease.
To achieve the goals above, the technical solution adopted by the present invention are as follows:
A kind of application of litsea cubeba oil on control capsicum epidemic disease.
Preferably, litsea cubeba oil is dissolved in organic solvent, adds emulsifying agent Tween, finally diluted with sterile water,
It mixes, obtains the medical fluid of required litsea cubeba oil concentration；Medical fluid is administered by the way of spraying or pouring root.
Preferably, when being administered by the way of spraying, 1 ~ 2 g of litsea cubeba oil, organic solvent are contained in every 1 L medical fluid
0.2 ~ 0.4 g, 1 ~ 2 g of emulsifying agent Tween.
Preferably, when being administered by the way of pouring root, containing 1.5 ~ 2.5 g of litsea cubeba oil, organic in every 1 L medical fluid
0.3 ~ 0.5 g of solvent, 1 ~ 3 g of emulsifying agent Tween.
Preferably, the preparation method of the litsea cubeba oil, comprising the following steps: fruit of a cubeb litsea tree fruit is dry, grinding, mistake
Sieve, obtains fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction
Condition: 40 ~ 50 DEG C of extraction temperature, extraction time 40 ~ 80 minutes, extracting pressure 2 ~ 3 MPa, CO21.2 ~ 2 mL/min of flow.
Preferably, the organic solvent is one or more of acetone, methanol, ethyl alcohol, propylene glycol and glycerol.
Preferably, the emulsifying agent Tween is Tween-60 or Tween-80.
The formation and release of P. capsici zoosporangium, EC not only can be effectively suppressed in litsea cubeba oil50Value point
Not Wei 29.68 mg/L and 207.96 mg/L, while sprouting to the zoospore discharged and mycelia growth also have it is fine
Inhibiting effect, EC50Value difference 90.68 mg/L and 161.49 mg/L.
Using medical fluid made of litsea cubeba oil of the present invention, have simultaneously to capsicum epidemic disease good protective effect and
Therapeutic effect is a kind of effectiveP. capsiciInhibitor, can be used for the comprehensive treatment of capsicum epidemic disease.Occur in disease
Different phase it can be used to be prevented and treated, and pathogen especially can be effectively suppressed at such medicament is applied before in sporangiform
It infects again and the developmenting spread of disease.
Specific embodiment
In order to keep technical purpose of the invention, technical scheme and beneficial effects clearer, combined with specific embodiments below
Technical solution of the present invention is further illustrated, but the embodiment is intended to explain the present invention, and should not be understood as pair
Limitation of the invention, in the examples where no specific technique or condition is specified, according to the literature in the art described technology or
Condition is carried out according to product description.
Fruit of a cubeb litsea tree fruit used is (fresh) from purchased from Jiangxi Province's Ji'an City Ji'an area country fair in following embodiments.
Embodiment 1
Litsea cubeba oil is dissolved in acetone, Tween-60 is added, finally with sterile water dilution, mixing, obtains required mountain
The medical fluid of grey sub- essential oil concentration.The concentration containing litsea cubeba oil is 1 g, 0.2 g of acetone, 1 g of Tween-60 in every 1 L medical fluid.
The preparation method of above-mentioned litsea cubeba oil, comprising the following steps: by the drying of fruit of a cubeb litsea tree fruit, be ground up, sieved, obtain
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions: extraction
Take temperature 45 C, extraction time 60 minutes, extracting pressure 2.5 MPa, CO21.5 mL/min of flow.
Embodiment 2
Litsea cubeba oil is dissolved in methanol, adds Tween-80, finally with sterile water dilution, mixing, it is grey to obtain required mountain
The medical fluid of sub- essential oil concentration.The concentration containing litsea cubeba oil is 1.5 g, 0.3 g of methanol, Tween-80 1.5 in every 1 L medical fluid
g。
The preparation method of above-mentioned litsea cubeba oil, comprising the following steps: by the drying of fruit of a cubeb litsea tree fruit, be ground up, sieved, obtain
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions: extraction
Take 40 DEG C of temperature, extraction time 80 minutes, extracting pressure 2 MPa, CO22 mL/min of flow.
Embodiment 3
Litsea cubeba oil is dissolved in ethyl alcohol, adds Tween-60, finally with sterile water dilution, mixing, it is grey to obtain required mountain
The medical fluid of sub- essential oil concentration.The concentration containing litsea cubeba oil is 2 g, 0.4 g of ethyl alcohol, 2 g of Tween-60 in every 1 L medical fluid.
The preparation method of above-mentioned litsea cubeba oil, comprising the following steps: by the drying of fruit of a cubeb litsea tree fruit, be ground up, sieved, obtain
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions: extraction
Take temperature 50 C, extraction time 40 minutes, extracting pressure 3 MPa, CO21.2 mL/min of flow.
Medical fluid made from embodiment 1 ~ 3 is applied to crop in such a way that whole strain is spraying.
Embodiment 4
Litsea cubeba oil is dissolved in propylene glycol, adds Tween-80, finally with sterile water dilution, mixing, obtains required mountain
The medical fluid of grey sub- essential oil concentration.The concentration containing litsea cubeba oil is 1.5 g, 0.3 g of propylene glycol, Tween-80 1 in every 1 L medical fluid
g。
The preparation method of above-mentioned litsea cubeba oil, comprising the following steps: by the drying of fruit of a cubeb litsea tree fruit, be ground up, sieved, obtain
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions: extraction
Take temperature 45 C, extraction time 60 minutes, extracting pressure 2.5 MPa, CO21.5 mL/min of flow.
Embodiment 5
Litsea cubeba oil is dissolved in glycerol, adds Tween-60, finally with sterile water dilution, mixing, it is grey to obtain required mountain
The medical fluid of sub- essential oil concentration.The concentration containing litsea cubeba oil is 2 g, 0.4 g of glycerol, 2 g of Tween-60 in every 1 L medical fluid.
The preparation method of above-mentioned litsea cubeba oil, comprising the following steps: by the drying of fruit of a cubeb litsea tree fruit, be ground up, sieved, obtain
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions: extraction
Take 40 DEG C of temperature, extraction time 80 minutes, extracting pressure 2 MPa, CO22 mL/min of flow.
Embodiment 6
Litsea cubeba oil is dissolved in ethyl alcohol, adds Tween-80, finally with sterile water dilution, mixing, it is grey to obtain required mountain
The medical fluid of sub- essential oil concentration.The concentration containing litsea cubeba oil is 2.5 g, 0.5 g of ethyl alcohol, 3 g of Tween-80 in every 1 L medical fluid.
The preparation method of above-mentioned litsea cubeba oil, comprising the following steps: by the drying of fruit of a cubeb litsea tree fruit, be ground up, sieved, obtain
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions: extraction
Take temperature 50 C, extraction time 40 minutes, extracting pressure 3 MPa, CO21.2 mL/min of flow.
Medical fluid made from embodiment 4 ~ 6 is applied to crop by the way of pouring root.
Effect test:
Test material: P. capsiciP. capsiciIt is formulated by Henan Province's new pesticide and is mentioned with priority application laboratory
For.V8 culture medium: 900 mL of deionized water, CaCO is added in 100 mLV8 juice3 1.5 g, 20 g of agar.V8 culture solution: 100
900 mL of deionized water, CaCO is added in mLV8 juice3 0.02 g。
The preparation of experimental liquid: 3 g litsea cubeba oils, 0.2 g acetone and 1 g Tween-60 are mixed, and diluted with sterile water
To 1L, the litsea cubeba oil experimental liquid that concentration is 3000 mg/L is obtained, experimental liquid 11 is denoted as；By the sterile moisture of experimental liquid 11
Be not diluted to litsea cubeba oil concentration be 25 mg/L, 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L, 250 mg/L,
500 mg/L, 600 mg/L, 1000 mg/L and 2000 mg/L are denoted as experimental liquid 1, experimental liquid 2, experimental liquid 3, experimental liquid respectively
4, experimental liquid 5, experimental liquid 6, experimental liquid 7, experimental liquid 8, experimental liquid 9, experimental liquid 10.
The configuration of comparison liquid: 0.2 g acetone and 1 g Tween-60 are mixed, and are diluted to 1 L with sterile water to get control
Liquid 1；0.3 g acetone and 1 g Tween-60 are mixed, and are diluted to 1 L with sterile water to get comparison liquid 2.
1. the inhibition virulence that litsea cubeba oil grows phytophthora blight of pepper mycelia
1 mL of experimental liquid of various concentration is taken to be added in the culture dish containing 9 mL V8 culture mediums (9 cm of diameter) respectively.With
Punch beats the Phytophthora capsici bacteria cake for taking diameter to be 5 mm, and each culture dish places 1 bacteria cake, is control with comparison liquid 1, often
Processing is repeated 3 times.Be placed in 25 DEG C of constant incubators and cultivate, when compare colony diameter it is long to 7 cm or more when, handed over cross
Fork method measures colony diameter, calculates mycelial growth inhibition rate, and concrete outcome is shown in Table 1.
The inhibiting effect that 1 litsea cubeba oil of table grows phytophthora blight of pepper mycelia
Phytophthora capsici mycelia is grown by research litsea cubeba oil virulence it is found that litsea cubeba oil to phytophthora blight of pepper
Mycelia growth has significant inhibiting effect, wherein 200 mg/L and the inhibiting rate of 300 mg/L two processing respectively reach
78.40% and 91.98%.
2. the influence that litsea cubeba oil forms phytophthora blight of pepper zoosporangium
The phytophthora blight of pepper for cultivating 3 d is taken out, 10 pieces of bacteria cake of 5 mm of diameter are cut from colony edge 1/3, are placed in
In sterilizes culture dish (9 cm of diameter), the V8 juice for pouring into 15 mL just floods mycelia block, and 25 DEG C of 2 d of dark culturing wait for mycelia clump
It grows.Mycelia clump is applied rinsed with sterile water 3 times, respectively 10 mL experimental liquids 1 of each addition, experimental liquid 2, experimental liquid 3, experimental liquid 4,
25 DEG C of 2 d of full exposure culture are control with comparison liquid 2.Each processing is repeated 3 times.After 24 h, each repetition randomly selects 3
The visual field counts sporangium number, calculates sporangiform into inhibiting rate, the results are shown in Table 2.
The influence that 2 litsea cubeba oil of table forms P. capsici zoosporangium
As shown in Table 2, the formation of P. capsici zoosporangium, and essential oil concentration can be effectively suppressed in litsea cubeba oil
Higher, inhibiting effect is stronger.Under the processing of low concentration litsea cubeba oil, Phytophthora capsici sporangium has different degrees of germination rate, but
When essential oil concentration is increased to 150 mg/L, then sporangium can hardly be formed.Further test finds out essential oil to Phytophthora capsici
Sporangiform at virulence regression equation beY=0.6928+2.9252X, related coefficient 0.9910, EC50Value is 29.68 mg/
L。
3. the influence that litsea cubeba oil discharges phytophthora blight of pepper zoosporangium
The mycelia for having generated a large amount of sporangiums clump is chosen into conical flask, 10 mL sterile waters are added, is stirred, is made with glass bar
Zoosporangium shakes off, and chooses mycelia block, as zoosporangium suspension.Draw respectively 30 μ L comparison liquids 1, experimental liquid 3,
Experimental liquid 5, experimental liquid 8, experimental liquid 9, experimental liquid 10 are added on the concave slide containing 30 μ L sporangia suspensions, by recessed glass
Piece is placed in moisturizing in the culture dish containing wet filter paper, through 4 DEG C of 30 min of low-temperature treatment, moves in 25 DEG C of insulating boxs and cultivates 5 h, take
Concave slide 3 visuals field of casual inspection under 10 × 10 power microscopes out, about 50 sporangiums are observed in each visual field, with zoospore
Zoospore is all swum out of into ghost and is recorded as discharging completely in capsule, calculates sporangium germination rate.3 repetitions of every processing, knot
Fruit is as shown in table 3.
The influence that 3 litsea cubeba oil of table discharges phytophthora blight of pepper zoosporangium
As can be seen from Table 3, various concentration litsea cubeba oil has the release of Phytophthora Capsici Zoospores capsule different degrees of
It influences, and as essential oil concentration increases, sporangium burst size reduces.It is right when litsea cubeba oil concentration is higher than 300 mg/L
The inhibiting rate of sporangium release is more than 50%, and when essential oil concentration is 1000 mg/L, sporangium release inhibiting rate is increased to
97.91%.Thus finding out the virulence regression equation that litsea cubeba oil discharges phytophthora blight of pepper sporangium isY=-0.8810+
2.5371X, correlation coefficient r value is 0.9593, EC50Value is 207.96 mg/L.
4. the influence that litsea cubeba oil sprouts P. capsici zoospore
The influence that P. capsici zoospore is sprouted using sessile drop method measurement litsea cubeba oil.It will generate a large amount of
The mycelia of sporangium, which is first placed under 4 DEG C of low temperature, handles 30 min, 25 DEG C of 1 h of constant incubator culture, and mycelia clump is chosen, is collected
Spore suspension.30 μ L comparison liquids 1, experimental liquid 3, experimental liquid 5, experimental liquid 8, experimental liquid 9, experimental liquid 10 is drawn respectively to be added
Onto the concave slide containing 30 μ L spore suspensions, concave slide is placed in moisturizing in the culture dish containing wet filter paper, every processing weight
It is 3 times multiple, 25 DEG C of 3 h of dark culturing.After compareing spore germination rate greater than 80%, the germination rate of all processing is checked, calculate spore
Germination suppression rate, and concentration EC in virulence regression equation and inhibition is asked according to probit analysis50Value, the results are shown in Table 4.
The influence that 4 litsea cubeba oil of table sprouts P. capsici zoospore
Table 4 shows inhibitory activity that litsea cubeba oil sprouts phytophthora blight of pepper zoospore with the increase of drug concentration
And increase.It is 52.44% to the inhibiting rate that zoospore is sprouted, when essential oil concentration is increased to when essential oil concentration is 100 mg/L
When 500 mg/L, to zoospore sprout inhibiting rate then up to 82.44%.Litsea cubeba oil inhibits Phytophthora Capsici Zoospores to sprout
The EC of hair50Value is 90.68 mg/L, and toxicity regression linear equation isY=-2.2061+1.4273X(r=0.9876).
5. spray-on process measures litsea cubeba oil to the field control effect of capsicum epidemic disease
This test arrangement is in the heavier field of Zhengzhou City Henan Province the Yellow River fishing ground capsicum epidemic disease morbidity over the years.Comparison medicament
250 grams per liter Fluoxastrobin suspending agents are provided by pharmacy Hebei, North China Hua Nuo Co., Ltd；Sweet tea big pimento in Henan is by Henan Yu Yizhong industry section
Skill Development Co., Ltd provides.
Totally 5 processing (including clear water control), 4 repetitions, 20 cells, random district's groups arrange for test.Every plot area
About 20 m2, 2 row of minizone interval is as protection row.When occurring at the beginning of capsicum epidemic disease, with 16A type back carried hand pressurization spray
Device carries out even spraying to the whole strain of capsicum, and liquid volume is every 1.6 kg of cell.It is successively carried out from low concentration to high concentration when spraying.
15 days and 30 days after spray, investigates respectively and the diseased plant number, dead strain number and obviously withered plant number for recording every cell calculate not
With the diseased plant rate and control efficiency of processing.Concrete outcome is shown in Table 5.
Control efficiency (spray-on process) of 5 litsea cubeba oil of table to capsicum epidemic disease
As can be seen from Table 5, the whole strain of capsicum is sprayed using spray-on process, 2000 mg/L litsea cubeba oils are to capsicum
The control efficiency of epidemic disease is suitable with the preventive effect of comparison medicament up to 75.62%；1500 mg/L and 1000 mg/L litsea cubeba oils
Though preventive effect substantially less than comparison medicament, to capsicum epidemic disease for this destructive soil-borne disease, preventive effect up to 65% with
On, it is worthy of popularization.
6. root-pouring method measures litsea cubeba oil to the field control effect of capsicum epidemic disease
This test arrangement is in the heavier field of Zhengzhou City Henan Province the Yellow River fishing ground capsicum epidemic disease morbidity over the years.Comparison medicament
250 grams per liter Fluoxastrobin suspending agents are provided by pharmacy Hebei, North China Hua Nuo Co., Ltd；Sweet tea big pimento in Henan is by Henan Yu Yizhong industry section
Skill Development Co., Ltd provides.
Totally 5 processing (including clear water control), 4 repetitions, 20 cells, random district's groups arrange for test.Every plot area
About 20 m2, 2 row of minizone interval is as protection row.When occurring at the beginning of capsicum epidemic disease, with syringe by 50 mL different disposals
Medical fluid is in the pouring root at 3 cm of rhizome.15 days and 30 days after medicine, 5 points of every cell random searching, every is looked into 5 plants, record disease
Strain number, dead strain number and obviously withered plant number, calculate the diseased plant rate and control efficiency of different disposal.Concrete outcome is shown in Table 6.
Control efficiency (root-pouring method) of 6 litsea cubeba oil of table to capsicum epidemic disease
As can be seen from Table 6, it is administered using root-pouring method, 2500 mg/L and 2000 mg/L litsea cubeba oils are to capsicum
The control efficiency of epidemic disease is suitable with the preventive effect of comparison medicament up to 80.37% and 74.90% respectively；1500 mg/L litsea cubeba oils
Though the preventive effect of preventive effect and substantially less than comparison medicament and high concentration processing, dense in the preventive effect of capsicum epidemic disease up to 65% or more
Spend the preventive effect indifference of processing.
To sum up, the shape of P. capsici zoosporangium not only can be effectively suppressed in this experimental study discovery litsea cubeba oil
At and release, EC50Value is respectively 29.68 mgL-1With 207.96 mgL-1, while sprouting to the zoospore discharged
Hair and mycelia growth also have good inhibiting effect, EC5090.68 mgL of value difference-1With 161.49 mgL-1；It is spraying
Method and the field control effectiveness test result of root-pouring method show that the essential oil has good control efficiency to capsicum epidemic disease, are that one kind has
EffectP. capsiciInhibitor, can be used for the comprehensive treatment of capsicum epidemic disease.It can be used in the different phase that disease occurs
It is prevented and treated, especially sporangiform at apply before such medicament can be effectively suppressed pathogen infect again and the hair of disease
Exhibition sprawling.
The above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although by referring to of the invention preferred
Invention has been described for embodiment, it should be appreciated by those of ordinary skill in the art that can in form and carefully
Various changes are made to it on section, without departing from the spirit and scope of the present invention defined by the appended claims.
Claims (2)
1. a kind of application of litsea cubeba oil on control capsicum epidemic disease, it is characterised in that: the litsea cubeba oil is to capsicum epidemic disease
The inhibition of mildew bacterium；Litsea cubeba oil is dissolved in organic solvent, adds emulsifying agent Tween, is finally diluted with sterile water, is mixed
It is even, obtain the medical fluid of required litsea cubeba oil concentration；Medical fluid is administered by the way of spraying or pouring root；
When being administered by the way of spraying, in every 1 L medical fluid containing 1 ~ 2 g of litsea cubeba oil, 0.2 ~ 0.4 g of organic solvent,
1 ~ 2 g of emulsifying agent Tween；
Or when being administered by the way of pouring root, in every 1 L medical fluid containing 1.5 ~ 2.5 g of litsea cubeba oil, organic solvent 0.3 ~
0.5 g, 1 ~ 3 g of emulsifying agent Tween；
The preparation method of the litsea cubeba oil, comprising the following steps: by the drying of fruit of a cubeb litsea tree fruit, be ground up, sieved, it is grey to obtain mountain
Sub- powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions: extraction temperature
40 ~ 50 DEG C of degree, extraction time 40 ~ 80 minutes, extracting pressure 2 ~ 3 MPa, CO21.2 ~ 2 mL/min of flow；
The organic solvent is one or more of acetone, methanol, ethyl alcohol, propylene glycol and glycerol.
2. application of the litsea cubeba oil on control capsicum epidemic disease according to claim 1, it is characterised in that: the tween type
Emulsifier is Tween-60 or Tween-80.
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| CN111713552B (en) * | 2020-07-24 | 2022-05-31 | 暨南大学 | Litsea cubeba essential oil sustained-release preparation and application thereof in airing and night storage of agricultural products |
| CN112940864A (en) * | 2021-02-02 | 2021-06-11 | 云南省农业科学院农产品加工研究所 | Microcapsule slow-release litsea essential oil applicable to meat product preservation |
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