CN106538624A - A kind of coptis bactericide and its application in preventing and treating crop epidemic disease - Google Patents
A kind of coptis bactericide and its application in preventing and treating crop epidemic disease Download PDFInfo
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- CN106538624A CN106538624A CN201610931548.7A CN201610931548A CN106538624A CN 106538624 A CN106538624 A CN 106538624A CN 201610931548 A CN201610931548 A CN 201610931548A CN 106538624 A CN106538624 A CN 106538624A
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- coptis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
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- Mycology (AREA)
- Plant Pathology (AREA)
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- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of coptis bactericide and its application in preventing and treating crop epidemic disease, the bactericide is Chinese Drug Rhizomes of Coptis ethanol extract.The coptis agent of the present invention has no toxic side effect from natural, is not easily caused pathogenic microorganism and produces anti-drug resistance, can be used to prevent and treat soybean blight, capsicum epidemic disease, the late blight of potato and watermelon epidemic disease；The direct extracting process of ethanol is simple, and cost is relatively low；Its extract, to person poultry safety, is a kind of low toxicity, effectively antibacterial biological pesticide.Therefore, there are huge potentiality as bactericide with the coptis.
Description
Technical field
The present invention relates to a kind of coptis bactericide and its application in preventing and treating crop epidemic disease.
Background technology
Oomycetes is to cause one of important pathogen of vegetable disease, and the parasitic scope of oomycetes is very wide, on nearly all vegetables
There is oomycetes disease.Oomycetes is destructive strong, causing harm property is big, quickly grow, by the more difficult preventing and treating mostly of the microbial vegetable disease of ovum.
Oomycetes disease is the class disease in Phycomycetes fungi, belongs to Mastigomycotina, is the important phytopathogen of a class, this kind of disease
Extensively, destructive strong, harm is very big, due to its short incubation period, then infects often, in a life for substance host crop of falling ill
Long season interior energy fast development causes plant disease epidemic, is easily caused crop and damages product mistake receipts.Such as 1847 in Ireland because of potato
Late blight is big to cause potato in poor harvest, and the big famine for causing causes nearly million people dead, and more than 200 ten thousand people lead a wretched life far from home.Closely
Over a little years, China's greenhouse vegetables cultivated area constantly increases, and what oomycetes disease was produced to vegetables causes harm also be on the rise.By oomycetes
The each middle disease for causing all is the very strong species of harmfulness, though species is few, morbidity is rapid, propagate fast, weight of causing harm.
Crop production reduction caused by plant disease receive less be agricultural development main obstruction.People are difficult to this for many years
The preventing and treating of topic, based on chemical prevention, is mainly the material of non-naturally occurring yet with chemical pesticide mostly, and is often difficult drop
Solution, residual life are longer, have destructive effects to natural, ecological, easily form public hazards, and some even jeopardize human health.Therefore,
Bioactivator is found in plant or directly exploitation botanical pesticide has become one of the focus in current pesticide research field.
Wherein, jamaicin can effectively prevent and treat the late blight of potato；Meng Zhaoli etc. is isolated from ginkgo very high to phytopathogen
The compound of activity, Jing Structural Identifications, manual simulation's synthesis, successfully develops " green Supreme Being " agricultural bactericidal agent series products；Stone Chang
Pu extract is to botrytis cinerea and Melon fusarium Wilt；Little rose extract is to botrytis cinerea mycelial growth inhibition rate
100% is reached；Angelica Dahurica extract energy strong inhibition botrytis cinerea spore germination, inhibiting rate is up to 91.59%；Fructus meliae toosendan,
Little rose and dandelion extract are to the inhibiting rate of ash arrhizus bacteria spore germination more than 70%.Therefore, find a kind of low toxicity, have
The preventing and treating that oomycetes growth and the biological pesticide for causing a disease are applied to crop epidemic disease that suppresses of effect is just very likely to.
The content of the invention
The invention provides a kind of economic, safe efficient, environmentally friendly coptis bactericide and its preventing and treating crop epidemic disease
In application, the coptis bactericide can be used to prevent and treat soybean blight, capsicum epidemic disease, the late blight of potato and watermelon epidemic disease,
Fungistatic effect significantly, disclosure satisfy that the demand of production.
For achieving the above object, the present invention is adopted the following technical scheme that：
The coptis bactericide of the present invention is coptis ethanol extract.The preparation process of the coptis ethanol extract is as follows：
With Chinese Drug Rhizomes of Coptis as raw material, plant sample is ground with mortar, add 75% ethanol of volumetric concentration of 10 times of volumes of plant sample, use
Shaking table concussion extraction 24h, is centrifuged to obtain supernatant and residue；7-8 times of volume of plant sample is added in the residue that first time put forward
75% ethanol of volumetric concentration, concussion extraction 18h, supernatant and residue is centrifuged to obtain；5-6 times of body of plant sample is added in residue
Long-pending 75% ethanol of volumetric concentration, concussion extraction 12h, is centrifuged to obtain supernatant and residue；Merge three supernatants, use rotary evaporation
Instrument carries out concentrated in vacuo（35℃,0.08MPa）, obtain concentrate；With filtering with microporous membrane it is degerming after, plus sterilized water constant volume arrives
1g/ml, is stored in -4 DEG C.
Described coptis bactericide is in preventing and treating soybean blight, capsicum epidemic disease, the late blight of potato and watermelon epidemic disease
Application.
The coptis bactericide of the present invention can be used to prevent and treat soybean blight, capsicum epidemic disease, the late blight of potato and watermelon
Epidemic disease, disease control using putting prevention first, the principle of medication in advance, when high temperature, super-humid conditions preventive usage and disease are sent out and
Early medication, additional 0.03% (V/V) SilwetL-77 of liquid are sprayed up and down to blade face, are not dripped as degree with blade face, and seedling is to opening
Flower, sprays 4 times, and visual incidence suitably increases and decreases number of times.It is watered 5-10 times to dilute during actual mist.
The remarkable advantage of the present invention:
The Chinese medicine of the present invention has no toxic side effect from natural, is not easily caused pathogenic microorganism and produces anti-drug resistance, can be used for
Preventing and treating soybean blight, capsicum epidemic disease, the late blight of potato and watermelon epidemic disease, the process of Jing coptiss bactericide, can reduce morbidity
Rate 75%-85%, is a kind of low toxicity, effectively antibacterial biological pesticide, and preparation method is simple, cost is relatively low.
Description of the drawings
Fig. 1 is inhibitory action of the coptis bactericide to four kinds of different phytophthora bacteria growings.
Fig. 2 is morphology influence of the coptis bactericide to phytophthora blight of pepper, and A is normal process CK, and B is that cloves is processed
The phytophthora crossed.
Specific embodiment
In order that content of the present invention easily facilitates understanding, with reference to specific embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Embodiment 1：It is raw material from Chinese Drug Rhizomes of Coptis, detects which to soybean blight, capsicum epidemic disease, the late blight of potato and west
The growth of melon epidemic disease bacterium and the inhibitory action of cellular morphology.The plant sample that mortar grinds, adds 10 times of volumes of plant sample
75% ethanol of volumetric concentration, with shaking table concussion extraction 24h, is centrifuged to obtain supernatant and residue；Add in the residue that first time carried
75% ethanol of volumetric concentration of 7-8 times of volume of plant sample, concussion extraction 18h, is centrifuged to obtain supernatant and residue；Add in residue
Enter 75% ethanol of volumetric concentration of 5-6 times of volume of plant sample, concussion extraction 12h is centrifuged to obtain supernatant and residue；Merge three times
Supernatant, is carried out with Rotary Evaporators concentrated in vacuo（35℃,0.08MPa）, obtain concentrate；It is degerming with filtering with microporous membrane
Afterwards, plus sterilized water constant volume is to 1g/ml, -4 DEG C are stored in.On V8 culture mediums, four kinds of growth of pathogenic bacteria counting rates find, for examination
When amount concentration is 1g/ml, the coptis to the growth of all strains testeds and cellular morphology inhibitory action effect substantially, especially to west
The inhibiting rate of melon phytophthora and phytophthora infestans nearly reaches 100%, as shown in Figure 1.
Embodiment 2：It is raw material from Chinese Drug Rhizomes of Coptis, detects which to soybean blight, capsicum epidemic disease, the late blight of potato and west
The prevention effect of melon epidemic disease.The coptis sample that mortar grinds, adds 75% ethanol of volumetric concentration of 10 times of volumes of plant sample, uses
Shaking table concussion extraction 24h, is centrifuged to obtain supernatant and residue；7-8 times of volume of plant sample is added in the residue that first time put forward
75% ethanol of volumetric concentration, concussion extraction 18h, supernatant and residue is centrifuged to obtain；5-6 times of body of plant sample is added in residue
Long-pending 75% ethanol of volumetric concentration, concussion extraction 12h, is centrifuged to obtain supernatant and residue；Merge three supernatants, use rotary evaporation
Instrument carries out concentrated in vacuo（35℃,0.08MPa）, obtain concentrate 1g/ml.Liquid additional 0.03% (V/V) SilwetL-77 pair
Blade face is sprayed up and down, is not dripped with blade face and is advisable, and seedling is sprayed 4 times to blooming, and visual incidence suitably increases and decreases number of times.It is real
Border is watered 5-10 times when spraying and dilutes.Prevention effect contrast finds that the coptis processes and can reduce incidence of disease 75%-85%.
The above results show, the Chinese Drug Rhizomes of Coptis ethanol extract obtained by this method, to soybean blight, capsicum epidemic disease,
The growth of the late blight of potato and watermelon epidemic disease and cause a disease have obvious inhibiting effect, this explanation coptis have as bactericide
Huge potentiality, while can also promote the preventing and treating of other crop epidemic diseases.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (4)
1. a kind of coptis bactericide, it is characterised in that：The bactericide is Chinese Drug Rhizomes of Coptis ethanol extract.
2. coptis bactericide according to claim 1, it is characterised in that：The preparation step of the coptis ethanol extract
It is rapid as follows：With Chinese Drug Rhizomes of Coptis as raw material, plant sample is ground with mortar, add the volumetric concentration 75% of 10 times of volumes of plant sample
Ethanol, with shaking table concussion extraction 24h, is centrifuged to obtain supernatant and residue；Plant sample 7-8 is added in the residue that first time carried
75% ethanol of volumetric concentration of times volume, concussion extraction 18h, is centrifuged to obtain supernatant and residue；Plant sample 5- is added in residue
75% ethanol of volumetric concentration of 6 times of volumes, concussion extraction 12h, is centrifuged to obtain supernatant and residue；Merge three supernatants, with rotation
Evaporimeter is concentrated under 0.08Mpa, is obtained concentrate at 35 DEG C；With filtering with microporous membrane it is degerming after, plus sterilized water constant volume arrives
1g/ml, is stored in -4 DEG C.
3. a kind of application of coptis bactericide as claimed in claim 1 or 2, it is characterised in that：The coptis plant kills
Microbial inoculum is infected the application in the crop epidemic disease for causing in preventing and treating by oomycetes.
4. the application of coptis bactericide according to claim 3, it is characterised in that：The crop epidemic disease is soybean
Epidemic disease, capsicum epidemic disease, the late blight of potato and watermelon epidemic disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610931548.7A CN106538624A (en) | 2016-10-31 | 2016-10-31 | A kind of coptis bactericide and its application in preventing and treating crop epidemic disease |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610931548.7A CN106538624A (en) | 2016-10-31 | 2016-10-31 | A kind of coptis bactericide and its application in preventing and treating crop epidemic disease |
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| CN106538624A true CN106538624A (en) | 2017-03-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041093A (en) * | 2017-12-08 | 2018-05-18 | 柳州雅瑞科技有限公司 | A kind of botanical fungicide for preventing edible lily epidemic disease and preparation method thereof |
| CN109090158A (en) * | 2018-09-27 | 2018-12-28 | 乐山师范学院 | A kind of preparation method and its control method preparing konjak soft rot protective agents using the coptis |
| CN108684734B (en) * | 2018-05-03 | 2020-06-05 | 安徽大学 | Compound preparation for inhibiting cotton mold growth |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041093A (en) * | 2017-12-08 | 2018-05-18 | 柳州雅瑞科技有限公司 | A kind of botanical fungicide for preventing edible lily epidemic disease and preparation method thereof |
| CN108684734B (en) * | 2018-05-03 | 2020-06-05 | 安徽大学 | Compound preparation for inhibiting cotton mold growth |
| CN109090158A (en) * | 2018-09-27 | 2018-12-28 | 乐山师范学院 | A kind of preparation method and its control method preparing konjak soft rot protective agents using the coptis |
| CN109090158B (en) * | 2018-09-27 | 2021-01-26 | 乐山师范学院 | Preparation method of medicine for preventing and treating soft rot of konjak prepared from coptis chinensis and prevention and treatment method thereof |
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Application publication date: 20170329 |
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