CN106508981A - Clove plant bactericide and application of clove plant bactericide in prevention and treatment of crop diseases - Google Patents
Clove plant bactericide and application of clove plant bactericide in prevention and treatment of crop diseases Download PDFInfo
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- CN106508981A CN106508981A CN201610931211.6A CN201610931211A CN106508981A CN 106508981 A CN106508981 A CN 106508981A CN 201610931211 A CN201610931211 A CN 201610931211A CN 106508981 A CN106508981 A CN 106508981A
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- Prior art keywords
- bactericide
- flos caryophylli
- residue
- ethanol
- clove
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a clove plant bactericide and application of the clove plant bactericide in prevention and treatment of crop diseases. The plant bactericide is a traditional Chinese medicine clove ethanol extracting solution. The clove plant bactericide is derived from the nature, the toxic and side effects are avoided, pathogenic microorganisms are not prone to being generated, the anti-drug resistance is produced, and the clove plant bactericide can be used for preventing and treating soybean blight, pepper blight, potato late blight and watermelon blight; an ethanol direct extracting method is simple, easy to achieve and low in cost; and a clove extract is safe to human and livestock and is low-toxicity and effective antibacterial biological pesticide. Therefore, using of cloves as plant bactericides has great potential.
Description
Technical field
The present invention relates to a kind of Flos Caryophylli bactericide and its application in preventing and treating crop epidemic disease.
Background technology
Oomycetes is to cause one of important pathogen of vegetable disease, and the parasitic scope of oomycetes is very wide, on nearly all vegetable
There is oomycetes disease.Oomycetes is destructive strong, causing harm property is big, quickly grow, by the more difficult preventing and treating mostly of the microbial vegetable disease of ovum.
Oomycetes disease is the class disease in Phycomycetes funguses, belongs to Mastigomycotina, is the important phytopathogen of a class, this kind of disease
Extensively, destructive strong, harm is very big, due to its short incubation period, then infects often, in a life for substance host crop of falling ill
Long season interior energy fast development causes plant disease epidemic, is easily caused crop and damages product mistake receipts.Such as 1847 in Ireland because of Rhizoma Solani tuber osi
Late blight is big to cause Rhizoma Solani tuber osi in poor harvest, and the big famine for causing causes nearly million people dead, and more than 200 ten thousand people lead a wretched life far from home.Closely
Over a little years, China's greenhouse vegetables cultivated area constantly increases, and what oomycetes disease was produced to vegetable causes harm also be on the rise.By oomycetes
The each middle disease for causing all is the very strong species of hazardness, though species is few, morbidity is rapid, propagate fast, weight of causing harm.
Crop production reduction caused by pathogenic fungus diseases receive less be agricultural development main obstruction.People couple for many years
The preventing and treating of this difficult problem, based on chemical prevention, is mainly the material of non-naturally occurring yet with chemical pesticide mostly, and often
Not degradable, residual period, is longer, has destructive effects to natural, ecological, easily forms public hazards, and some even jeopardize human health.
Therefore, finding bioactive substance or direct exploitation botanical pesticide in plant has become the focus in current pesticide research field
One of.Wherein, berberine can effectively prevent and treat the late blight of potato；Meng Zhaoli etc. is isolated from Semen Ginkgo to be had to phytopathogen
Very highly active compound, Jing Structural Identifications, manual simulation synthesis, successfully develops " green Supreme Being " agricultural bactericidal agent series products；
Rhizoma Acori Graminei extract is to botrytis cinerea and Melon fusarium Wilt；Little rose extract presses down to botrytis cinerea mycelial growth
Rate processed reaches 100%；Radix Angelicae Dahuricae extract energy strong inhibition botrytis cinerea spore germination, suppression ratio is up to 91.59%；Fructus Toosendan
Sub, little Flos Rosae Rugosae and Herba Taraxaci extract are to the suppression ratio of ash arrhizus bacteria spore germination more than 70%.Therefore, find a kind of low
Poison, the effectively growth of suppression oomycetes and the biological pesticide for causing a disease are applied to the preventing and treating of crop epidemic disease and are just very likely to.
The content of the invention
The invention provides a kind of economic, safe efficient, environmentally friendly Flos Caryophylli bactericide and its preventing and treating crop epidemic disease
In application, the Flos Caryophylli bactericide can be used to prevent and treat soybean blight, capsicum epidemic disease, the late blight of potato and Citrullus vulgariss epidemic disease,
Fungistatic effect significantly, disclosure satisfy that the demand of production.
For achieving the above object, the present invention is adopted the following technical scheme that：
The Flos Caryophylli bactericide of the present invention is Flos Caryophylli ethanol extract.The preparation process of the Flos Caryophylli ethanol extract is as follows：
With Chinese medicine Flos Caryophylli as raw material, plant sample is ground with mortar, add 75% ethanol of volumetric concentration of 10 times of volumes of plant sample, use
Shaking table concussion extraction 24h, is centrifuged to obtain supernatant and residue；7-8 times of volume of plant sample is added in the residue that first time put forward
75% ethanol of volumetric concentration, concussion extraction 18h, supernatant and residue is centrifuged to obtain；5-6 times of body of plant sample is added in residue
Long-pending 75% ethanol of volumetric concentration, concussion extraction 12h, is centrifuged to obtain supernatant and residue；Merge three supernatant, use rotary evaporation
Instrument carries out concentrated in vacuo（35℃,0.08MPa）, obtain concentrated solution；With filtering with microporous membrane it is degerming after, plus sterilized water constant volume arrives
1g/ml, is stored in -4 DEG C.
Described Flos Caryophylli bactericide is in preventing and treating soybean blight, capsicum epidemic disease, the late blight of potato and Citrullus vulgariss epidemic disease
Application.
The Flos Caryophylli bactericide of the present invention can be used to prevent and treat soybean blight, capsicum epidemic disease, the late blight of potato and Citrullus vulgariss
Epidemic disease, disease control using putting prevention first, the principle of medication in advance, when high temperature, super-humid conditions preventive usage and disease are sent out and
Early medication, additional 0.03% (V/V) SilwetL-77 of medicinal liquid are sprayed up and down to blade face, are not dripped as degree with blade face, and seedling is to opening
Flower, sprays 4 times, and visual incidence suitably increases and decreases number of times.It is watered 5-10 times to dilute during actual mist.
The remarkable advantage of the present invention:
The Chinese medicine of the present invention has no toxic side effect from natural, is not easily caused pathogenic microorganism and produces anti-drug resistance, can be used for
Preventing and treating soybean blight, capsicum epidemic disease, the late blight of potato and Citrullus vulgariss epidemic disease, the process of Jing Flos Caryophyllis bactericide, can reduce morbidity
Rate 50%-60%, is a kind of low toxicity, effectively antibacterial biological pesticide, and preparation method is simple, cost is relatively low.
Description of the drawings
Fig. 1 is inhibitory action of the Flos Caryophylli bactericide to four kinds of different phytophthora bacteria growings.
Fig. 2 is morphology influence of the Flos Caryophylli bactericide to phytophthora blight of pepper, and A is normal process CK, and B is that Flos Caryophylli is processed
The phytophthora crossed.
Specific embodiment
In order that content of the present invention easily facilitates understanding, with reference to specific embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Embodiment 1：It is raw material from Chinese medicine Flos Caryophylli, detects which to soybean blight, capsicum epidemic disease, the late blight of potato and west
The growth of melon epidemic disease bacterium and the inhibitory action of cellular morphology.The plant sample that mortar grinds, adds 10 times of volumes of plant sample
75% ethanol of volumetric concentration, with shaking table concussion extraction 24h, is centrifuged to obtain supernatant and residue；Add in the residue that first time carried
75% ethanol of volumetric concentration of 7-8 times of volume of plant sample, concussion extraction 18h, is centrifuged to obtain supernatant and residue；Add in residue
Enter 75% ethanol of volumetric concentration of 5-6 times of volume of plant sample, concussion extraction 12h is centrifuged to obtain supernatant and residue；Merge three times
Supernatant, is carried out with Rotary Evaporators concentrated in vacuo（35℃,0.08MPa）, obtain concentrated solution；It is degerming with filtering with microporous membrane
Afterwards, plus sterilized water constant volume is to 1g/ml, -4 DEG C are stored in.In V8 culture medium, four kinds of growth of pathogenic bacteria counting rates find, for examination
When amount concentration is 1g/ml, Flos Caryophylli to the growth of all strains testeds and cellular morphology inhibitory action effect substantially, especially to causing
The suppression ratio of sick phytophthora nearly reaches 100%, as shown in Figure 1.
Embodiment 2：It is raw material from Chinese medicine Flos Caryophylli, detects which to soybean blight, capsicum epidemic disease, the late blight of potato and west
The prevention effect of melon epidemic disease.The plant sample that mortar grinds, adds 75% ethanol of volumetric concentration of 10 times of volumes of plant sample, uses
Shaking table concussion extraction 24h, is centrifuged to obtain supernatant and residue；7-8 times of volume of plant sample is added in the residue that first time put forward
75% ethanol of volumetric concentration, concussion extraction 18h, supernatant and residue is centrifuged to obtain；5-6 times of body of plant sample is added in residue
Long-pending 75% ethanol of volumetric concentration, concussion extraction 12h, is centrifuged to obtain supernatant and residue；Merge three supernatant, use rotary evaporation
Instrument carries out concentrated in vacuo（35℃,0.08MPa）, obtain concentrated solution 1g/ml.Medicinal liquid additional 0.03% (V/V) SilwetL-77 pair
Blade face is sprayed up and down, is not dripped with blade face and is advisable, and seedling is sprayed 4 times to blooming, and visual incidence suitably increases and decreases number of times.It is real
5-10 times is watered during the mist of border to dilute.Prevention effect contrast finds that Flos Caryophylli processes and can reduce sickness rate 50%-60%.
The above results show, the Chinese medicine Flos Caryophylli ethanol extract obtained by this method, to soybean blight, capsicum epidemic disease,
The growth of the late blight of potato and Citrullus vulgariss epidemic disease and cause a disease have obvious inhibiting effect, this explanation Flos Caryophylli have as bactericide
Huge potentiality, while can also promote the preventing and treating of other crop epidemic diseases.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (4)
1. a kind of Flos Caryophylli bactericide, it is characterised in that：The bactericide is Chinese medicine Flos Caryophylli ethanol extract.
2. Flos Caryophylli bactericide according to claim 1, it is characterised in that：The preparation step of the Flos Caryophylli ethanol extract
It is rapid as follows：With Chinese medicine Flos Caryophylli as raw material, plant sample is ground with mortar, add the volumetric concentration 75% of 10 times of volumes of plant sample
Ethanol, with shaking table concussion extraction 24h, is centrifuged to obtain supernatant and residue；Plant sample 7-8 is added in the residue that first time carried
75% ethanol of volumetric concentration of times volume, concussion extraction 18h, is centrifuged to obtain supernatant and residue；Plant sample 5- is added in residue
75% ethanol of volumetric concentration of 6 times of volumes, concussion extraction 12h, is centrifuged to obtain supernatant and residue；Merge three supernatant, with rotation
Evaporimeter is concentrated under 0.08Mpa, is obtained concentrated solution at 35 DEG C；With filtering with microporous membrane it is degerming after, plus sterilized water constant volume arrives
1g/ml, is stored in -4 DEG C.
3. a kind of application of Flos Caryophylli bactericide as claimed in claim 1 or 2, it is characterised in that：The Flos Caryophylli plant kills
Microbial inoculum is infected the application in the crop epidemic disease for causing in preventing and treating by oomycetes.
4. the application of Flos Caryophylli bactericide according to claim 3, it is characterised in that：The crop epidemic disease is Semen sojae atricolor
Epidemic disease, capsicum epidemic disease, the late blight of potato and Citrullus vulgariss epidemic disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610931211.6A CN106508981A (en) | 2016-10-31 | 2016-10-31 | Clove plant bactericide and application of clove plant bactericide in prevention and treatment of crop diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610931211.6A CN106508981A (en) | 2016-10-31 | 2016-10-31 | Clove plant bactericide and application of clove plant bactericide in prevention and treatment of crop diseases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106508981A true CN106508981A (en) | 2017-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610931211.6A Pending CN106508981A (en) | 2016-10-31 | 2016-10-31 | Clove plant bactericide and application of clove plant bactericide in prevention and treatment of crop diseases |
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| Country | Link |
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| CN (1) | CN106508981A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114027300A (en) * | 2021-11-01 | 2022-02-11 | 中国农业科学院农业基因组研究所 | Application of isoeugenol and methylated derivative metabolite thereof in inhibiting phytophthora infestans and preventing and treating plant diseases |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940206A (en) * | 2010-09-30 | 2011-01-12 | 江苏天容集团股份有限公司 | Application of eugenol in preventing and controlling plant diseases and bactericide |
| CN102037966A (en) * | 2010-12-31 | 2011-05-04 | 山东亿嘉农化有限公司 | Eugenol biological pesticide and preparation method thereof |
| CN104430583A (en) * | 2014-11-27 | 2015-03-25 | 馥稷生物科技发展（上海）有限公司 | Pesticide composition containing clove and litsea cubeba extracts as well as preparation method and application of pesticide composition |
-
2016
- 2016-10-31 CN CN201610931211.6A patent/CN106508981A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940206A (en) * | 2010-09-30 | 2011-01-12 | 江苏天容集团股份有限公司 | Application of eugenol in preventing and controlling plant diseases and bactericide |
| CN102037966A (en) * | 2010-12-31 | 2011-05-04 | 山东亿嘉农化有限公司 | Eugenol biological pesticide and preparation method thereof |
| CN104430583A (en) * | 2014-11-27 | 2015-03-25 | 馥稷生物科技发展（上海）有限公司 | Pesticide composition containing clove and litsea cubeba extracts as well as preparation method and application of pesticide composition |
Non-Patent Citations (3)
| Title |
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| 唐春红: "《天然防腐剂与抗氧化剂》", 31 May 2010, 中国轻工业出版社 * |
| 曾志红等: "植物提取物对龙眼主要致腐真菌的抑制作用", 《森林与环境学报》 * |
| 陈仕江等: "10种中药植物杀菌活性研究", 《重庆中草药研究》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114027300A (en) * | 2021-11-01 | 2022-02-11 | 中国农业科学院农业基因组研究所 | Application of isoeugenol and methylated derivative metabolite thereof in inhibiting phytophthora infestans and preventing and treating plant diseases |
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Application publication date: 20170322 |