CN107232238A - A kind of application of litsea cubeba oil on control capsicum epidemic disease - Google Patents
A kind of application of litsea cubeba oil on control capsicum epidemic disease Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention discloses a kind of application of litsea cubeba oil on control capsicum epidemic disease, litsea cubeba oil is dissolved in organic solvent, emulsifying agent Tween is added, is finally diluted, mixed with sterilized water, the decoction of litsea cubeba oil concentration needed for obtaining；Decoction is administered by the way of spraying or pouring root；Wherein, the litsea cubeba oil is prepared by following steps：Fruit of a cubeb litsea tree fruit is dried, grind, sieved, fruit of a cubeb litsea tree powder is obtained；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil.The decoction being made of litsea cubeba oil of the present invention, is a kind of inhibitor of effective phytophthora blight of pepper, has good field control effect to capsicum epidemic disease, the comprehensive regulation available for capsicum epidemic disease.Disease occur different phase it can be used to be prevented and treated, especially sporangiform into before administration such medicament can effectively suppress pathogen infect again and disease developmenting spread.
Description
Technical field
The invention belongs to cultural control technical field, and in particular to a kind of litsea cubeba oil answering on control capsicum epidemic disease
With.
Background technology
Litsea cubeba oil is extracted from Lauraceae Litsea machaka fruit of a cubeb litsea tree Litsea cubeba Pers fresh fruits
Volatile oil, be the edible natural spices that GB2760-2011 is defined as allowing to use, be that China single variety export volume is maximum
Plants essential oil.《Dictionary of medicinal plant》Record its have expelling wind and clearing away cold, qi-regulating desinsection, detoxify analgesic effect, be common medicine among the people it
One.Initially, week brave wait finds that litsea cubeba oil is a kind of broad-spectrum antifungal medicine by experimental study, and confirms that it has sterilization to make
With.Then, the research to litsea citrate oil antibacterial action and mechanism is increasing, but is concentrated mainly on food and medical domain.It is remaining
Uncle is good to wait research to show litsea cubeba oil to aspergillus flavus common in food（Aspergillus flauus）, Penicillium citrinum
（Penicillium citrinum）, Mucor racemosus（Mucor racemosus）And bread mold（Rhizopus nigricans）
There is stronger antibacterial action etc. a variety of moulds, the food such as peanut, the corn of easy contaminated mold and aflatoxin can be used as
Good natural antisepsis fresh-keeping agent.Litsea cubeba oil is to candida albicans（Candida albicans）, trichophyta（Trichophyton
rubrum）, aspergillus fumigatus（Aspergillus fumigatus）Fungi and staphylococcus aureus are planted Deng more than ten
（Staphyloccocus aureus）, gonococcus（Neisseria gonorrhoeae）And Escherichia coli（Escherichia
coli）There are suppression and killing action well Deng more than ten kind malignant bacterias, be the day of known antimicrobial spectrum most wide and forceful drug effect
One of right plant extracts.
At present, it is less to the research report of plant pathogenic fungi bioactivity about litsea cubeba oil.A variety of millet figured woven silk material age etc. grinds
Study carefully and find that litsea citrate oil has very strong suppression plant pathogenic fungi Fusarium oxysporum（Fusarium oxysporum）、Helminthosporium sp. , StemphyilliumSp. the ability grown, and have obvious preservation；Yuan Ping etc.
Report that litsea cubeba oil has stronger inhibitory activity to mould.
By phytophthora blight of pepper（Phytophthora capsici）Caused plant disease is given birth to as vegetables all over the world
The main restricting factor of production.The various vegetables and other plant of germ harm Solanaceae, Curcurbitaceae and pulse family amount to kind more than 50, can
Each position of plant is infected, causes the symptoms such as withered plant, root-rot, neckrot, seedling is dampinged off, leaf is withered, stem is withered, fruit corruption.By capsicum
Phytophthora infects caused capsicum epidemic disease has turned into a kind of crushing soil-borne fungus venereal disease evil at present, there is generation all over the world,
The lighter field diseased plant rate of morbidity is 15 ~ 30%, and it is 20 ~ 25% to cause production loss, and serious field is lost more than 50%, very
To total crop failure.There is the report that capsicum epidemic disease seriously occurs in China's pepper planting regional such as Sichuan, Guizhou, Shaanxi, Gansu, Guangdong
Road, and with the increase of protecting field pepper planting area, capsicum epidemic disease has the trend aggravated year by year.In recent years, it is
The loss that capsicum epidemic disease is caused is reduced, capsicum epidemic disease is prevented in terms of genetic breeding, tillage control measure, chemical prevention both at home and abroad
Control and carried out numerous studies, but do not find Resistant gerplasm material at present；The Agricultural management system for preventing and treating capsicum epidemic disease is more complicated,
Large area production cultivation is difficult to carry out；It is still to use bactericide that the most frequently used measure of capsicum epidemic disease is prevented and treated in production.20th century 80
Systemic fungicide metalaxyl, Sandofan M8, cyazofamid etc. is begun to use to prevent and treat capsicum epidemic disease effect preferably after age, but with
The long-term use of these medicaments, has caused the drug-fast generation of germ, and a large amount of using environmental pollution is easily caused, and threatens food peace
Entirely, human health is caused harm.
The content of the invention
Object of the present invention is to provide application of the litsea cubeba oil on control crop capsicum epidemic disease.
To achieve these goals, the technical solution adopted by the present invention is：
A kind of application of litsea cubeba oil on control capsicum epidemic disease.
Preferably, litsea cubeba oil is dissolved in organic solvent, adds emulsifying agent Tween, finally diluted with sterilized water,
Mix, the decoction of litsea cubeba oil concentration needed for obtaining；Decoction is administered by the way of spraying or pouring root.
Preferably, when being administered by the way of spraying, the g of litsea cubeba oil 1 ~ 2, organic solvent are contained in every 1 L decoctions
0.2 ~ 0.4 g, the g of emulsifying agent Tween 1 ~ 2.
Preferably, when being administered by the way of pouring root, containing the g of litsea cubeba oil 1.5 ~ 2.5, organic in every 1 L decoctions
The g of solvent 0.3 ~ 0.5, the g of emulsifying agent Tween 1 ~ 3.
Preferably, the preparation method of the litsea cubeba oil, comprises the following steps：By the drying of fruit of a cubeb litsea tree fruit, grinding, mistake
Sieve, obtains fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction
Condition：40 ~ 50 DEG C of extraction temperature, extraction time 40 ~ 80 minutes, extracting pressure 2 ~ 3 MPa, CO2The mL/min of flow 1.2 ~ 2.
Preferably, the organic solvent is more than one or both of acetone, methanol, ethanol, propane diols and glycerine.
Preferably, the emulsifying agent Tween is Tween-60 or Tween-80.
Litsea cubeba oil not only can effectively suppress the formation and release of P. capsici zoosporangium, its EC50Value point
Not Wei 29.68 mg/L and 207.96 mg/L, while the sprouting of zoospore and mycelial growth to having discharged also have it is fine
Inhibitory action, its EC50Value difference 90.68 mg/L and 161.49 mg/L.
The decoction being made of litsea cubeba oil of the present invention, to capsicum epidemic disease simultaneously have good protective effect and
Therapeutic action, is a kind of effectiveP. capsiciInhibitor, the comprehensive regulation available for capsicum epidemic disease.Occur in disease
Different phase it can be used to be prevented and treated, and especially can effectively suppress pathogen into such medicament of administration before in sporangiform
Infect again and disease developmenting spread.
Embodiment
In order that the technical purpose of the present invention, technical scheme and beneficial effect are clearer, with reference to specific embodiment
Technical scheme is further illustrated, but the embodiment is intended to explain the present invention, and it is not intended that right
The limitation of the present invention, in the examples where no specific technique or condition is specified, according to the technology described by document in the art or
Condition is carried out according to product description.
Fruit of a cubeb litsea tree fruit used in following embodiments（It is fresh）From purchased from Ji'an City Ji'an, Jiangxi Province area country fair.
Embodiment 1
Litsea cubeba oil is dissolved in acetone, Tween-60 is added, is finally diluted, mixed with sterilized water, the required fruit of a cubeb litsea tree is obtained
The decoction of essential oil concentration.The concentration containing litsea cubeba oil is 1 g, the g of acetone 0.2, the g of Tween-60 1 in every 1 L decoctions.
The preparation method of above-mentioned litsea cubeba oil, comprises the following steps：Fruit of a cubeb litsea tree fruit is dried, grind, sieved, is obtained
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions：Extraction
Take temperature 45 C, extraction time 60 minutes, extracting pressure 2.5 MPa, CO2The mL/min of flow 1.5.
Embodiment 2
Litsea cubeba oil is dissolved in methanol, Tween-80 is added, is finally diluted, mixed with sterilized water, fruit of a cubeb litsea tree essence needed for obtaining
The decoction of oil concentration.The concentration containing litsea cubeba oil is 1.5 g, the g of methanol 0.3, the g of Tween-80 1.5 in every 1 L decoctions.
The preparation method of above-mentioned litsea cubeba oil, comprises the following steps：Fruit of a cubeb litsea tree fruit is dried, grind, sieved, is obtained
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions：Extraction
Take 40 DEG C of temperature, extraction time 80 minutes, extracting pressure 2 MPa, CO2The mL/min of flow 2.
Embodiment 3
Litsea cubeba oil is dissolved in ethanol, Tween-60 is added, is finally diluted, mixed with sterilized water, fruit of a cubeb litsea tree essence needed for obtaining
The decoction of oil concentration.The concentration containing litsea cubeba oil is 2 g, the g of ethanol 0.4, the g of Tween-60 2 in every 1 L decoctions.
The preparation method of above-mentioned litsea cubeba oil, comprises the following steps：Fruit of a cubeb litsea tree fruit is dried, grind, sieved, is obtained
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions：Extraction
Take temperature 50 C, extraction time 40 minutes, extracting pressure 3 MPa, CO2The mL/min of flow 1.2.
Decoction made from embodiment 1 ~ 3 is applied to crop by the way of whole strain spraying.
Embodiment 4
Litsea cubeba oil is dissolved in propane diols, Tween-80 is added, is finally diluted, mixed with sterilized water, the required fruit of a cubeb litsea tree is obtained
The decoction of essential oil concentration.The concentration containing litsea cubeba oil is 1.5 g, the g of propane diols 0.3, the g of Tween-80 1 in every 1 L decoctions.
The preparation method of above-mentioned litsea cubeba oil, comprises the following steps：Fruit of a cubeb litsea tree fruit is dried, grind, sieved, is obtained
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions：Extraction
Take temperature 45 C, extraction time 60 minutes, extracting pressure 2.5 MPa, CO2The mL/min of flow 1.5.
Embodiment 5
Litsea cubeba oil is dissolved in glycerine, Tween-60 is added, is finally diluted, mixed with sterilized water, fruit of a cubeb litsea tree essence needed for obtaining
The decoction of oil concentration.The concentration containing litsea cubeba oil is 2 g, the g of glycerine 0.4, the g of Tween-60 2 in every 1 L decoctions.
The preparation method of above-mentioned litsea cubeba oil, comprises the following steps：Fruit of a cubeb litsea tree fruit is dried, grind, sieved, is obtained
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions：Extraction
Take 40 DEG C of temperature, extraction time 80 minutes, extracting pressure 2 MPa, CO2The mL/min of flow 2.
Embodiment 6
Litsea cubeba oil is dissolved in ethanol, Tween-80 is added, is finally diluted, mixed with sterilized water, fruit of a cubeb litsea tree essence needed for obtaining
The decoction of oil concentration.The concentration containing litsea cubeba oil is 2.5 g, the g of ethanol 0.5, the g of Tween-80 3 in every 1 L decoctions.
The preparation method of above-mentioned litsea cubeba oil, comprises the following steps：Fruit of a cubeb litsea tree fruit is dried, grind, sieved, is obtained
Fruit of a cubeb litsea tree powder；Fruit of a cubeb litsea tree powder is used into supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions：Extraction
Take temperature 50 C, extraction time 40 minutes, extracting pressure 3 MPa, CO2The mL/min of flow 1.2.
Decoction made from embodiment 4 ~ 6 is applied to crop by the way of pouring root.
Effect test：
Test material：P. capsiciP. capsiciThere is provided by the initiative of Henan Province's novel agrochemical with priority application laboratory.
V8 culture mediums：100 mLV8 juice add deionized water 900 mL, CaCO3 1.5 g, the g of agar 20.V8 nutrient solutions：100 mLV8 juice
Add deionized water 900 mL, CaCO3 0.02 g。
The preparation of experimental liquid：3 g litsea cubeba oils, 0.2 g acetone and 1 g Tween-60s are mixed, and diluted with sterilized water
To 1L, the litsea cubeba oil experimental liquid that concentration is 3000 mg/L is obtained, experimental liquid 11 is designated as；By the sterile moisture of experimental liquid 11
Be not diluted to litsea cubeba oil concentration for 25 mg/L, 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L, 250 mg/L,
500 mg/L, 600 mg/L, 1000 mg/L and 2000 mg/L, are designated as experimental liquid 1, experimental liquid 2, experimental liquid 3, experimental liquid respectively
4th, experimental liquid 5, experimental liquid 6, experimental liquid 7, experimental liquid 8, experimental liquid 9, experimental liquid 10.
The configuration of comparison liquid：0.2 g acetone and 1 g Tween-60s are mixed, and 1 L is diluted to sterilized water, control is produced
Liquid 1；0.3 g acetone and 1 g Tween-60s are mixed, and 1 L is diluted to sterilized water, comparison liquid 2 is produced.
1. litsea cubeba oil is to the suppression virulence of phytophthora blight of pepper mycelial growth
The mL of experimental liquid 1 of various concentrations is taken to be added to the culture dish containing 9 mL V8 culture mediums respectively（The cm of diameter 9）In.With punching
Device beats the Phytophthora capsici bacteria cake for taking a diameter of 5 mm, and each culture dish places 1 bacteria cake, is control with comparison liquid 1, often handles
It is repeated 3 times.It is placed in 25 DEG C of constant incubators and cultivates, when compareing colony diameter length to 7 more than cm, uses crossing method
Colony diameter is measured, mycelial growth inhibition rate is calculated, concrete outcome is shown in Table 1.
Inhibitory action of the litsea cubeba oil of table 1 to phytophthora blight of pepper mycelial growth
By studying the virulence that litsea cubeba oil grows to Phytophthora capsici mycelia, litsea cubeba oil is to phytophthora blight of pepper mycelia
Growth has significant inhibitory action, wherein the inhibiting rate of two processing of 200 mg/L and 300 mg/L respectively reaches 78.40% He
91.98%。
2. the influence that litsea cubeba oil is formed to phytophthora blight of pepper zoosporangium
The phytophthora blight of pepper for cultivating 3 d is taken out, the mm of diameter 5 10 pieces of bacteria cake is cut at colony edge 1/3, sterilizing is placed in
Culture dish（The cm of diameter 9）In, the V8 juice for pouring into 15 mL just floods mycelia block, and 25 DEG C of d of dark culturing 2 treat that mycelia clump grows.
Mycelia clump application rinsed with sterile water 3 times, 10 mL experimental liquids 1 of each addition, experimental liquid 2, experimental liquid 3,4,25 DEG C of experimental liquid are complete respectively
The d of illumination cultivation 2, is control with comparison liquid 2.Each processing is repeated 3 times.It is each to repeat to randomly select 3 visual field systems after 24 h
Sporangium number is counted, sporangiform is calculated into inhibiting rate, as a result as shown in table 2.
The influence that the litsea cubeba oil of table 2 is formed to P. capsici zoosporangium
As shown in Table 2, litsea cubeba oil can effectively suppress the formation of P. capsici zoosporangium, and essential oil concentration is got over
Height, inhibitory action is stronger.Under the processing of low concentration litsea cubeba oil, Phytophthora capsici sporangium has different degrees of germination rate, but works as
When essential oil concentration is increased to 150 mg/L, then sporangium can hardly be formed.Further experiment obtains essential oil to Phytophthora capsici spore
Ascus formed virulence regression equation beY=0.6928+2.9252X, coefficient correlation is 0.9910, EC50It is worth for 29.68 mg/L.
3. the influence that litsea cubeba oil discharges to phytophthora blight of pepper zoosporangium
The mycelia clump for having produced a large amount of sporangiums is chosen into conical flask, 10 mL sterilized waters is added, is stirred with glass bar, make to move about
Sporangium shakes off, and chooses mycelia block, as zoosporangium suspension.30 μ L comparison liquids 1, experimental liquid 3, experiment are drawn respectively
Liquid 5, experimental liquid 8, experimental liquid 9, experimental liquid 10 are added on the concave slide containing 30 μ L sporangia suspensions, and concave slide is put
The moisturizing in the culture dish containing wet filter paper, through 4 DEG C of min of low-temperature treatment 30, moves to and 5 h is cultivated in 25 DEG C of insulating boxs, takes out recessed
About 50 sporangiums are observed in slide 3 visuals field of casual inspection under 10 × 10 power microscopes, each visual field, with zoosporangium
Zoospore is all swum out of into ghost and is recorded as complete release, calculates sporangium germination rate.3 repetitions are often handled, as a result such as
Shown in table 3.
The influence that the litsea cubeba oil of table 3 discharges to phytophthora blight of pepper zoosporangium
As can be seen from Table 3, various concentrations litsea cubeba oil has different degrees of influence to the release of Phytophthora Capsici Zoospores capsule,
And as essential oil concentration increases, the reduction of sporangium burst size.When litsea cubeba oil concentration is higher than 300 mg/L, it is to sporangium
The inhibiting rate of release is more than 50%, when essential oil concentration is 1000 mg/L, and sporangium release inhibiting rate brings up to 97.91%.Thus
Obtain litsea cubeba oil is to the virulence regression equation that phytophthora blight of pepper sporangium dischargesY=-0.8810+2.5371X, phase relation
Number r values are 0.9593, EC50It is worth for 207.96 mg/L.
4. the influence that litsea cubeba oil is sprouted to P. capsici zoospore
The influence that litsea cubeba oil is sprouted to P. capsici zoospore is determined using sessile drop method.A large amount of spores will have been produced
The mycelia of capsule, which is first placed under 4 DEG C of low temperature, handles 30 min, 25 DEG C of h of constant incubator culture 1, and mycelia clump is chosen, spore is collected
Suspension.30 μ L comparison liquids 1, experimental liquid 3, experimental liquid 5, experimental liquid 8, experimental liquid 9, experimental liquid 10 is drawn respectively to be added to and contain
On the concave slide for there are 30 μ L spore suspensions, concave slide is placed in moisturizing in the culture dish containing wet filter paper, often processing repeats 3
It is secondary, 25 DEG C of h of dark culturing 3.After spore germination rate is compareed more than 80%, the germination rate of all processing is checked, spore is calculated and sprouts
Inhibiting rate is sent out, and concentration EC in virulence regression equation and suppression is asked according to probit analysis50Value, as a result as shown in table 4.
The influence that the litsea cubeba oil of table 4 is sprouted to P. capsici zoospore
Table 4 shows that litsea cubeba oil increases to the inhibitory activity that phytophthora blight of pepper zoospore is sprouted with the increase of drug concentration
Greatly.When essential oil concentration is 100 mg/L, the inhibiting rate sprouted to zoospore is 52.44%, when essential oil concentration brings up to 500
During mg/L, the inhibiting rate sprouted to zoospore is then up to 82.44%.Litsea cubeba oil suppresses what Phytophthora Capsici Zoospores were sprouted
EC50It is worth for 90.68 mg/L, toxicity regression linear equation isY=-2.2061+1.4273X（r=0.9876）.
5. spray-on process determines field control effect of the litsea cubeba oil to capsicum epidemic disease
This test arrangement is in the heavier field of Zhengzhou City Henan Province the Yellow River fishing ground capsicum epidemic disease morbidity over the years.250 grams of comparison medicament/
Fluoxastrobin suspending agent is risen to be provided by pharmacy Hebei, North China Hua Nuo Co., Ltds；Sweet tea big pimento in Henan is by the skill kind industry development in science and technology of Henan Henan
Co., Ltd provides.
Experiment totally 5 processing（Including clear water control）, 4 repetitions, 20 cells, random district's groups arrangement.Per plot area
About 20 m2, the row of minizone interval 2, which is used as, protects row.When occurring at the beginning of capsicum epidemic disease, with 16A type back carried hand pressurization sprays
Device carries out even spraying to the whole strain of capsicum, and amount of liquid medicine is per the kg of cell 1.6.Carried out successively from low concentration to high concentration during spraying.
15 days and 30 days after spray, the diseased plant number, dead strain number and substantially withered plant number investigated respectively and record every cell are calculated not
With the diseased plant rate and prevention effect of processing.Concrete outcome is shown in Table 5.
Prevention effect of the litsea cubeba oil of table 5 to capsicum epidemic disease（Spray-on process）
As can be seen from Table 5, the whole strain of capsicum is sprayed using spray-on process, 2000 mg/L litsea cubeba oils are to capsicum epidemic disease
Prevention effect up to 75.62%, and the preventive effect of comparison medicament is suitable；1500 mg/L and 1000 mg/L litsea cubeba oils preventive effect
Though substantially less than comparison medicament, to capsicum epidemic disease for this destructive soil-borne disease, its preventive effect is up to more than 65%, value
Obtain popularization and application.
6. root-pouring method determines field control effect of the litsea cubeba oil to capsicum epidemic disease
This test arrangement is in the heavier field of Zhengzhou City Henan Province the Yellow River fishing ground capsicum epidemic disease morbidity over the years.250 grams of comparison medicament/
Fluoxastrobin suspending agent is risen to be provided by pharmacy Hebei, North China Hua Nuo Co., Ltds；Sweet tea big pimento in Henan is by the skill kind industry development in science and technology of Henan Henan
Co., Ltd provides.
Experiment totally 5 processing（Including clear water control）, 4 repetitions, 20 cells, random district's groups arrangement.Per plot area
About 20 m2, the row of minizone interval 2, which is used as, protects row.When occurring at the beginning of capsicum epidemic disease, with syringe by 50 mL different disposals
Decoction is in the pouring root at the cm of rhizome 3.15 days and 30 days after medicine, per cell 5 points of random searching, every is looked into 5 plants, record disease
Strain number, dead strain number and substantially withered plant number, calculate the diseased plant rate and prevention effect of different disposal.Concrete outcome is shown in Table 6.
Prevention effect of the litsea cubeba oil of table 6 to capsicum epidemic disease（Root-pouring method）
As can be seen from Table 6, dispenser is carried out using root-pouring method, 2500 mg/L and 2000 mg/L litsea cubeba oils are to capsicum epidemic disease
Prevention effect it is suitable up to 80.37% and 74.90%, and the preventive effect of comparison medicament respectively；The preventive effect of 1500 mg/L litsea cubeba oils
Though the preventive effect handled with substantially less than comparison medicament and high concentration, to the preventive effect of capsicum epidemic disease up at more than 65%, with middle concentration
The preventive effect indifference of reason.
To sum up, this experimental study finds that litsea cubeba oil not only can effectively suppress the shape of P. capsici zoosporangium
Into and release, its EC50Value is respectively 29.68 mgL-1With 207.96 mgL-1, while sprouting to the zoospore that has discharged
Hair and mycelial growth also have good inhibiting effect, its EC5090.68 mgL of value difference-1With 161.49 mgL-1；Spraying
Method and the field control effectiveness test result of root-pouring method show that the essential oil has good prevention effect to capsicum epidemic disease, are that one kind has
EffectP. capsiciInhibitor, the comprehensive regulation available for capsicum epidemic disease.The different phase occurred in disease can be used
It is prevented and treated, especially sporangiform into before using such medicament can effectively suppress pathogen infect again and disease hair
Exhibition spreads.
The above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although by referring to the present invention it is preferred
Invention has been described for embodiment, it should be appreciated by those of ordinary skill in the art that can in form and carefully
Various changes are made to it on section, the spirit and scope of the present invention limited without departing from appended claims.
Claims (7)
1. a kind of application of litsea cubeba oil on control capsicum epidemic disease.
2. application of the litsea cubeba oil on control capsicum epidemic disease according to claim 1, it is characterised in that：By fruit of a cubeb litsea tree essence
Oil is dissolved in organic solvent, adds emulsifying agent Tween, is finally diluted, mixed with sterilized water, and litsea cubeba oil needed for obtaining is dense
The decoction of degree；Decoction is administered by the way of spraying or pouring root.
3. application of the litsea cubeba oil on control capsicum epidemic disease according to claim 2, it is characterised in that：Using spraying
When mode is administered, the g of litsea cubeba oil 1 ~ 2, the g of organic solvent 0.2 ~ 0.4, emulsifying agent Tween 1 ~ 2 are contained in every 1 L decoctions
g。
4. application of the litsea cubeba oil on control capsicum epidemic disease according to claim 2, it is characterised in that using pouring root
When mode is administered, containing the g of litsea cubeba oil 1.5 ~ 2.5, the g of organic solvent 0.3 ~ 0.5, tween type emulsification in every 1 L decoctions
The g of agent 1 ~ 3.
5. application of the litsea cubeba oil on control capsicum epidemic disease according to claim 2, it is characterised in that the fruit of a cubeb litsea tree
The preparation method of essential oil, comprises the following steps：Fruit of a cubeb litsea tree fruit is dried, grind, sieved, fruit of a cubeb litsea tree powder is obtained；By the fruit of a cubeb litsea tree
Powder uses supercritical CO2Extraction, obtains litsea cubeba oil；Wherein, supercritical CO2Extraction conditions：40 ~ 50 DEG C of extraction temperature, extraction
40 ~ 80 minutes time, extracting pressure 2 ~ 3 MPa, CO2The mL/min of flow 1.2 ~ 2.
6. application of the litsea cubeba oil on control capsicum epidemic disease according to claim 2, it is characterised in that：It is described organic molten
Agent is more than one or both of acetone, methanol, ethanol, propane diols and glycerine.
7. application of the litsea cubeba oil on control capsicum epidemic disease according to claim 2, it is characterised in that：The tween type
Emulsifying agent is Tween-60 or Tween-80.
Priority Applications (1)
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| CN111713552A (en) * | 2020-07-24 | 2020-09-29 | 暨南大学 | Litsea cubeba essential oil sustained-release preparation and application thereof in airing and night storage of agricultural products |
| CN112940864A (en) * | 2021-02-02 | 2021-06-11 | 云南省农业科学院农产品加工研究所 | Microcapsule slow-release litsea essential oil applicable to meat product preservation |
| CN116098172A (en) * | 2022-09-01 | 2023-05-12 | 江苏省中国科学院植物研究所 | Application of zanthoxylum schinifolium volatile oil in preventing and treating pepper epidemic disease |
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| CN112940864A (en) * | 2021-02-02 | 2021-06-11 | 云南省农业科学院农产品加工研究所 | Microcapsule slow-release litsea essential oil applicable to meat product preservation |
| CN116098172A (en) * | 2022-09-01 | 2023-05-12 | 江苏省中国科学院植物研究所 | Application of zanthoxylum schinifolium volatile oil in preventing and treating pepper epidemic disease |
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