CN104886168A

CN104886168A - Biopesticide for preventing and treating tomato bacterial wilt and preparation method thereof

Info

Publication number: CN104886168A
Application number: CN201510301887.2A
Authority: CN
Inventors: 胡凡营
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-06-04
Filing date: 2015-06-04
Publication date: 2015-09-09

Abstract

The invention discloses a biopesticide for preventing and treating tomato bacterial wilt and a preparation method thereof. The biopesticide is prepared from Chinese herbal medicines including Chinese holly, yellowweed, Chinese violet, sculellaria barbata, sanguisorba officinalis, rheum officinale and common andrographis herb. After the biopesticide is applied in the field for three times, the prevention and treatment effect on the tomato bacterial wilt is more than 80%, and is obviously better than that of 30% hymexazol. The biopesticide has no phytotoxicity and has good safety.

Description

A kind of biopesticide of control of plant bacterial wilt and preparation method

Technical field

The present invention relates to biological pesticide technical field, particularly relate to a kind of biopesticide and preparation method of control of plant bacterial wilt.

Background technology

Bacterial wilt of tomato, be that fibrovascular system venereal disease common on tomato one of does harm to, various places generally occur, and protecting field, open country all can occur.Southern and the rainy time occurs generally serious.Cause when falling ill serious that plant is blue or green witheredly to die, cause Severe Reduction even to have no harvest.The young plant phase sickevil symptom is not obvious, and after plant blossom, diseased plant starts to show hazard symptoms.Leaf color is thin out, in wilting shape.Blade is wilted first from upper blade, follows by lower blade, is finally middle part blade.Fall ill initial blade noon wilt, at dusk, recover normal, repeated multiple times morning, wilting aggravates, finally withered, but plant is still cyan.In sick stem, bottom cortex is coarse, often grows adventive root and indefinite bud, sick stem vascular bundleblackening brown, but diseased plant root is normal.Soak in clear water after the sick stem of crosscut or extrude otch with hand, having milky mucus to overflow.Current pesticide control mainly blue or green withered Garrick, dislike mould spirit, metalaxyl-mn-zn, 20% Yekuzuo etc.But due to a large amount of uses of chemical pesticide, make various harmful levels of pathogens create drug resistance, add difficulty of prevention and cure, meanwhile, have residual in vegetable melon and fruit, harm health of people, contaminated environment, the destruction ecological balance.

And botanical pesticide is as a part for biosynthesis agricultural chemicals, there is low toxicity, high, the easy degraded of selectivity, the advantage little on the impact of ecotope, and wide material sources, cheap, active ingredient in its extract, as a kind of environmental protection type agricultural chemical, has good DEVELOPMENT PROSPECT and using value.

Summary of the invention

The object of this invention is to provide a kind of noresidue, non-environmental-pollution, control efficiency significantly, a kind of biopesticide of control of plant bacterial wilt be made up of pure Chinese herbal medicine.

The object of the invention is to be achieved through the following technical solutions, this biopesticide is made up of following herbal raw material: Chinese holly 10-16 part, climbing groundsel 10-16 part, Chinese violet 10-16 part, Sculellaria barbata 10-16 part, garden burnet 20-26 part, rheum officinale 20-26 part, punching connect 20-26 part.

Preferred: Chinese holly 13 parts, climbing groundsel 13 parts, Chinese violet 13 parts, Sculellaria barbata 13 parts, garden burnet 23 parts, rheum officinale 23 parts, punching connect 23 parts.

Preparation method: produce by traditional alcoholic extraction method.

Field trial

Preparation for examination biopesticide: take Chinese holly 130 grams, climbing groundsel 130 grams, Chinese violet 130 grams, Sculellaria barbata 130 grams, garden burnet 230 grams, rheum officinale 230 grams, punching connect 230 grams, said herbal medicine is pressed reprovision neat, dry, pulverize, mix and use 95% alcohol, 4 d are extracted under normal temperature, after extract is centrifugal, vacuum distillation temperature is about 50 ~ 52 DEG C, by the vaporized alcohol in extracts of Chinese herbal medicine.Then be 1g/ml with distilled water constant volume to mass concentration, obtain this biopesticide.Above-mentioned biopesticide is diluted respectively 100 times of liquid, 150 times of liquid, 250 times of liquid, 400 times of liquid, with 30% dislike mould spiritcompare with clear water, carry out field control effectiveness test to bacterial wilt of tomato, so that beneficial effect of the present invention to be described better, result of the test is as shown in following table 1.

Experimental field being positioned at Linyi City the earth Demonstration Garden, there is bacterial wilt of tomato, tests in first 7 days and process of the test and do not use other agricultural chemicals in cucumber in greenhouse booth ground.Experimental field soil property is sandy loam, and rich water is sufficient, and management is consistent.Duration of test temperature at 25 ~ 35 degrees Celsius, mean temperature 28 degrees Celsius; 100 times of liquid of this biopesticide, 150 times of liquid, 250 times of liquid, 400 times of liquid, 30% is established in this field trial dislike mould spirit, clear water (CK).Totally 6 process, each process repetition 4 times, totally 24 process communities, often process community according to the medicament preparation listed by table 1, growing way and the basically identical field of morbidity is selected to start phase the 1st medication in bacterial wilt of tomato, after this every 7d medication 1 time, continuous 3 times, knapsack sprayer is used, amount of liquid medicine standard is 30kg/ mu, every strain blade and stem by medicine evenly, avoid liquid to drift about, depart from target.After first time dispenser, second time medicine is executed at interval after 7 days, then after 7 days, execute third time medicine, altogether dispenser three times.The 7th afternoon unified investigation bacterial wilt of tomato feelings, calculating disease index, control efficiency after each dispenser.4 points are evenly fixed in Cha Shimei community, often 4 strains, totally 16 strains.

Bacterial wilt of tomato incidence grade scale is as follows: 0 grade of complete stool is anosis; 1 grade of complete stool less than 25% stem, blade are fallen ill; 2 grades of complete stool 25% ~ 50% stems, blades are fallen ill; 3 grades of complete stool more than 50% stems, blades are fallen ill; 4 grades due to illness withered.Disease refers to calculate as follows with control efficiency:

Table 1 different disposal is to the control efficiency of bacterial wilt of tomato

Conclusive table 1 result of the test can draw, after three dispensers, biopesticide all more than 80%, dislikes mould spirit significantly better than 30% to the control efficiency of bacterial wilt of tomato.The process of 4 various dose of biopesticide is to the control efficiency of bacterial wilt of tomato along with the increase of spraying times is in rising trend, and effective drug duration is long.Experimental session is observed, and biopesticide is to tomato crop without poisoning, and safety is good.The above results shows biopesticide of the present invention Be very effective in control of plant bacterial wilt.

Embodiment

Embodiment one: take Chinese holly 130 grams, climbing groundsel 130 grams, Chinese violet 130 grams, Sculellaria barbata 130 grams, garden burnet 230 grams, rheum officinale 230 grams, punching connect 230 grams, said herbal medicine is pressed reprovision neat, dry, pulverize, mix and use 95% alcohol, 4 d are extracted under normal temperature, after extract is centrifugal, vacuum distillation temperature is about 50 ~ 52 DEG C, by the vaporized alcohol in extracts of Chinese herbal medicine.Then be 1g/ml with distilled water constant volume to mass concentration, obtain this biopesticide, be placed in 0 ~ 4 DEG C of refrigerator for subsequent use.

Embodiment two: take Chinese holly 140 grams, climbing groundsel 140 grams, Chinese violet 140 grams, Sculellaria barbata 140 grams, garden burnet 240 grams, rheum officinale 240 grams, punching connect 240 grams, preparation method is with embodiment one.

Embodiment three: take Chinese holly 120 grams, climbing groundsel 120 grams, Chinese violet 120 grams, Sculellaria barbata 120 grams, garden burnet 220 grams, rheum officinale 220 grams, punching connect 220 grams, preparation method is with embodiment one.

Claims

1. a biopesticide for control of plant bacterial wilt, is characterized in that this biopesticide is made up of following herbal raw material: Chinese holly 10-16 part, climbing groundsel 10-16 part, Chinese violet 10-16 part, Sculellaria barbata 10-16 part, garden burnet 20-26 part, rheum officinale 20-26 part, punching connect 20-26 part.

2. the biopesticide of a kind of control of plant bacterial wilt as claimed in claim 1, is characterized in that this biopesticide is made up of following herbal raw material: Chinese holly 13 parts, climbing groundsel 13 parts, Chinese violet 13 parts, Sculellaria barbata 13 parts, garden burnet 23 parts, rheum officinale 23 parts, punching connect 23 parts.

3. the preparation method of the biopesticide of a control of plant bacterial wilt, it is characterized in that Chinese herbal medicine being dried, pulverizing, mix and extract 4d with under 95% alcohol normal temperature, after extract is centrifugal, vacuum distillation temperature is about 50 ~ 52 DEG C, by the vaporized alcohol in extracts of Chinese herbal medicine, is then 1g/ml with distilled water constant volume to mass concentration, obtain this biopesticide, be placed in 0 ~ 4 DEG C of refrigerator for subsequent use.