CN104705354B - A kind of gardens plant insecticide - Google Patents
A kind of gardens plant insecticide Download PDFInfo
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- CN104705354B CN104705354B CN201510129552.7A CN201510129552A CN104705354B CN 104705354 B CN104705354 B CN 104705354B CN 201510129552 A CN201510129552 A CN 201510129552A CN 104705354 B CN104705354 B CN 104705354B
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Abstract
A kind of gardens plant insecticide, belongs to technical field of insecticide.It is characterized in that by weight than following material composition：Plumage awns chrysanthemum extract, the careless extract of lob, Le party pepper extract, southerm yew leaf extract, Berberine hydrochloride, Blumea balsamifera extraction of essential oil thing are according to weight than 10 20:10‑20:10‑20:5‑15:1‑2:25 ratio mixing.A kind of above-mentioned gardens plant insecticide, its processing method is simple, and production cost is low, good disinsection effect.
Description
Technical field
The invention belongs to technical field of insecticide, specially a kind of gardens plant insecticide.
Background technology
Plant insecticide by its active ingredient activity, chemical constitution and purposes etc., be generally divided into alkaloid, terpenes,
Essential oil class, their modes of action to insect are varied, and such as poisoning, deterrent, food refusal, suppression are grown, and destruction insect is believed
Breath transmission and mating, anesthesia and the suppression effect of growing etc..Plants essential oil is that molecule amount is smaller, can be steamed with vapor
Go out, with certain volatile oily liquids material, some plants essential oils have good expelling parasite, insecticidal action, had to insect
It is certain lure, the effect such as repellent, suppression are grown.Plants essential oil is in addition to having higher activity to insect, also with to people
The advantages of nontoxic, free from environmental pollution, insect is not likely to produce the resistance to the action of a drug is raiseeed, therefore is that a kind of plant source for having broad prospect of application is killed
Worm agent.
The exploitation of plant insecticide can be divided into two aspects：One is direct utilization, i.e., to the active material in plant
After slightly being carried, available preparation is processed into.The characteristics of this Land use systems, can play various composition in crude extract
Synergy, and small investment, the construction cycle are short.Two be indirect utilization, i.e. the structure of research active material, mechanism of action, structure
Effect relation, carries out manual simulation's synthesis screening, therefrom develops novel plant source pesticide preparation, both Land use systems are all China
The direction of botanical pesticide research and development.
Plant insecticide can be made that emulsion, pulvis are used alone or several plant agricultural chemicals is used cooperatively, can also be with
Biological pesticide, chemical pesticide mixture are used.Because plant insecticide is low to people and animals' toxicity, easily degraded in the environment, substantially
It is upper without obvious residual hazard problem.Therefore, botanical pesticide is applied to vegetables, tealeaves, Chinese medicine, flower plant and urban afforestation
Ornamental plant preventing and treating pest and disease damage in terms of, have broad prospects.
Plumage awns chrysanthemumTridax procumbensL is composite family long handle Dendranthema, is distributed in tropical America, propagates afterwards in print
Degree, South East Asia Mainland and Indonesia.It is wild in China Guangdong, Taiwan, oneself ease of Hainan, and wild resource is very abundant.Text
Report is offered, plumage awns chrysanthemum has heat-clearing, removing toxic substances, the effect of dampness removing.The effect of plumage awns chrysanthemum leaf and flower with anti-inflammation, is frequently used for
Incised wound, scratch, wound, wound hemostasis, while alopecia can also be prevented effectively.Research table of the early stage to plumage awns chrysanthemum chemical composition
It is bright, the compositions such as flavones, cumarin, triterpenes, aromatic series volatile oil are mainly contained in plumage awns chrysanthemum, flavones ingredient therein has
Stronger antioxidation activity.Plumage awns chrysanthemum is Alien Invasive Plants, and experiment is also confirmed, the plant has stronger allelopathy and plant
Thing detoxifying function.
Lob grassHyptis rhomboideaMart. et Gal are Labiatae bee saage platymiscium.This platymiscium has 350-
400 kinds, american torrid zone is originated to subtropical zone.China has 4 kinds, sees south China coastal.Lob grass is among the people
For treating hepatitis, ulcer and oedema.The volatile oil of lob grass has preferable antibacterial, anti-oxidant and cytotoxic activity.Its
Main component contains flavones, the composition such as triterpenic acid.Lob grass is Alien Invasive Plants, and experiment is also confirmed, the plant has stronger
Allelopathy and phytotoxin effect.
Blumea balsamiferaBlumea balsamiferaBelong to fragrant plant for composite family Blumea balsamifera, be a kind of with middle benefit gas promoting blood circulation, dispel
The herbal medicine that wind dehumidifies with insecticidal action.Can be clinically used for treatment arthralgia pain due to rheumatism, cold-dampness rush down dysentery, stomachache borborygmus, venomous snake bite,
Arthralgia and myalgia, traumatic injury, tinea sore, anemofrigid cold.Its biological scholarship and moral conduct happiness temperature climate, it is drought-resistant, it is suitable for acid soil
Growth, barren-resistant, side can typically be planted than more loose, well-drained soil.
Strangle party's Chinese prickly ashZanthoxylumavicennaeFor Rutaceae xanthoxylum, China be mainly distributed on Hainan,
The southern areas such as Fujian, Guangdong, Taiwan, Guangxi, Yunnan.In Sanya, Hainan, strangle party's Chinese prickly ash resource distribution and enrich.Document report rue
The compositions such as volatile oil, flavones, alkaloid, cumarin, acid amides are mainly contained in fragrant section's xanthoxylum, Chinese prickly ash ether is clinically used
Extract or pepper volatile oil carry out anti-inflammatory analgetic, obtain good curative effect.According to another document report, xanthoxylum is to 4 kinds of depths
Portion fungi and 11 kinds of dermatophytes have certain antibacterial and bactericidal action.Find that pepper volatile oil can suppress H in addition22Liver cancer is thin
Born of the same parents' propagation and activated cell apoptosis, thus it is speculated that it has certain antitumor action.
Chinese yew (Taxus mairei) for taxaceae (Taxaceae) Taxus (Taxus genus) plant
Thing, also known as Japanese yew, Chi Baisong.Contain various taxanes analog in Chinese yew, wherein more than 10 kind compositions have antitumor activity.
Contained taxanes belongs to Diterpenes liposoluble constituent in southerm yew, dissolves in chloroform, acetone, ethanol, water insoluble.
Experiment shows that southerm yew needle ethanol extract has very strong antitumor action, in addition, the water extract of needle has
Certain phytotoxin effect, while also pointing out in the Chinese yew dregs of a decoction after refining taxol, it is more likely that containing with it is above-mentioned
Composition identical water solubility allelopathic composition in infusion.If being natural herbicide by the allelopathic composition exploitation in these dregs of a decoction,
Not only direction can be found for the resource reutilization of the Chinese yew dregs of a decoction, also comply with the ecological circulation pattern of refuse reclamation, specifically
Research also needs to deep exploration.
Allelopathy (counteracting each other, Allelopathy) refers to the metabolic secretion thing of plant or microorganism in environment
Neighbouring other plant or microorganism produce beneficial and harmful influence.Now, Allelopathic Effect in Plants research has been expanded to plant
Centered on all organisms and environment between pass through chemical substance be medium chemical interaction.In recent years, vegetalization is studied
Sense effect has turned into forest conservation, harmful organism control, increase crop yield and the important way for promoting continuity of environment sexual development
One of footpath.
With continuing to develop that modern science and technology are studied, people have filtered out a large amount of plants with allelopathy
Resource.Allelopathic Effect in Plants affects competition and the succession of community of plant of plant kingdom's inter-species.Therefore finding has allelopathy to show
The allelochemical of elephant, it is a new way having a extensive future to be controlled weeds using allelopathy, excavated plant insecticide.Due to
These chemical substances with allelopathy are easily degraded in plant, toxicity is low, the mode of action is unique, target selectivity is high, are not had
There is the environmental problem of traditional residues of pesticides etc., will not also insect is developed immunity to drugs, pest and disease damage can be efficiently controlled, will not band
Carry out the problem of environmental pollutions such as soil.
The allelopathy of plant is phytotoxin effect, and allelochemical is possible to substituting chemical pesticide and killed as plant source
Worm agent, at least greatly reduces chemical pesticide consumption.Therefore, agricultural production can be mitigated to ring using natural plants exploitation insecticide
Chemical pesticide residual quantity in the pressure in border, reduction crops, therefore harmful organism is prevented and treated using the allelochemical of plant, understand into
One of developing direction for plant protection.
The content of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design to provide a kind of gardens plant source
The technical scheme of insecticide, its processing method is simple, and production cost is low, and prevention effect is good.
A kind of described gardens plant insecticide, it is characterised in that by weight than following material composition：Plumage awns chrysanthemum
Extract, the careless extract of lob, Le party pepper extract, southerm yew leaf extract, Berberine hydrochloride, Blumea balsamifera essential oil are carried
Thing is taken to compare 10-20 according to weight:10-20:10-20: 5-15: 1-2:2-5 ratio mixing；
Described plumage awns chrysanthemum extract is prepared using following methods：Crushed after being dried is placed in container into the plumage awns chrysanthemum of coarse powder
It is interior, the distillation water boiling and extraction of addition plumage awns chrysanthemum 4-8 times of weight of coarse powder 1-2 times, each 0.5-1.5 hours, suction filtration discards filter
Slag, merges after extract solution, and obtained extract solution carries out flash concentration extremely under the conditions of 60-80 DEG C using Reduced Pressure Concentration Device
The 1/10-1/14 of original volume, dry powder is dried in vacuo into by concentrate again, produces plumage awns chrysanthemum extract dry powder；
The careless extract of described lob is prepared using following methods：Careless crushed after being dried will be lobbed into coarse powder, according to lob
Careless coarse powder 1kg then ethanol consumption for 6-10 rise ratio add 50% EtOH Sonicate extract 2-3 times, it is each 30-50 minutes；
Suction filtration, discards filter residue, merges after extract solution, obtained extract solution is entered under the conditions of 60-70 DEG C using Reduced Pressure Concentration Device
Concentrate is spray-dried or is dried in vacuo into dry powder to the 1/10-1/14 of original volume, hung by row flash concentration
Ball grass extract dry powder；
Described Le party pepper extract is prepared using following methods：Party's Chinese prickly ash crushed after being dried will be strangled into coarse powder, according to
Strangle party Chinese prickly ash coarse powder 1kg then ethanol consumption for 8-12 rise ratio add 95% EtOH Sonicate extract 2-3 times, each 20-40
Minute；Suction filtration, discards filter residue, merges after extract solution, and obtained extract solution concentrates dress under the conditions of 55-65 DEG C using vacuum film
Progress flash concentration is put to the 1/8-1/12 of original volume, concentrate is spray-dried or be dried in vacuo into dry powder, is obtained
To Le party pepper extract dry powder；
Described southerm yew leaf extract is prepared using following methods：By leaves of Taxus mairei crushed after being dried into thick
Powder, according to southerm yew leaf coarse powder 1kg then ethanol consumption for 6-10 rise ratio add 50% EtOH Sonicate extract 2-3
It is secondary, it is each 1-2 hours；Suction filtration, discards filter residue, merges after extract solution, and obtained extract solution uses vacuum under the conditions of 60-70 DEG C
Film enrichment facility carries out flash concentration to the 1/10-1/14 of original volume, is spray-dried or carried out vacuum by concentrate and do
It is dry into dry powder, obtain southerm yew leaf extract dry powder；
Described Blumea balsamifera extraction of essential oil thing is prepared using following methods：Crushed after being dried is put into the Blumea balsamifera of coarse powder
In flask, the distilled water of Blumea balsamifera 3 times of weight of coarse powder is added, volatile oil extractor is loaded onto, is carried using steam distillation formulation
Essential oil is taken, untill liquid clarification is slipped out, Blumea balsamifera extraction of essential oil thing is obtained.
Described a kind of gardens plant insecticide, it is characterised in that plumage awns chrysanthemum extract, the careless extract of lob, Le party
Pepper extract, southerm yew leaf extract, Berberine hydrochloride, the weight ratio of Blumea balsamifera extraction of essential oil thing are 12-18:12-
18:12-18:6-12: 1.2-1.8:2.5-4.5。
Described a kind of gardens plant insecticide, it is characterised in that plumage awns chrysanthemum extract, the careless extract of lob, Le party
Pepper extract, southerm yew leaf extract, Berberine hydrochloride, the weight ratio of Blumea balsamifera extraction of essential oil thing are 14-16:14-
16:14-16:8-10: 1.4-1.6:3-4。
Described a kind of gardens plant insecticide, it is characterised in that in described plumage awns chrysanthemum extract：Add distillation
Decocting time after water is each 0.5-1.5 hours；Extract solution is 65-75 DEG C in flash concentration temperature, is concentrated into the 1/ of original volume
11-1/13。
Described a kind of gardens plant insecticide, it is characterised in that in the careless extract of described lob：Ultrasonic extraction
Time is 35-45 minutes every time, and flash concentration temperature is 62-65 DEG C, is concentrated into the 1/11-1/13 of original volume.
Described a kind of gardens plant insecticide, it is characterised in that in described Le party pepper extract：Ultrasound is carried
Take the time each 20-40 minutes, flash concentration temperature is 58-60 DEG C, is concentrated into the 1/9-1/11 of original volume.
Described a kind of gardens plant insecticide, it is characterised in that in described southerm yew leaf extract：It is super
Sound extraction time is each 1.2-1.5 hours；Flash concentration temperature is 62-65 DEG C, is concentrated into the 1/11-1/13 of original volume.
A kind of above-mentioned gardens plant insecticide, its processing method is simple, and production cost is low, with following beneficial effect
Really：
1）For ornamental plant, usually containing various larvas and microorganism, the growth and development of plant is influenceed, synthesis is used
Insecticide, medicament residue can be caused, using the insecticide for coming from plant source, without any synthesis articles for use, to phytopathy
Insect pest has very strong insecticidal action, and insect difficulty develops immunity to drugs, and plant is not damaged in itself, and non agricultural chemical residuum, selectivity are strong,
It is nontoxic to people and animals, pollution-free, using safe and efficient；
2）This method advanced technology, easy to operate, technique is simple, and recovery rate is high, and production cost is low, extracts and makes in concentrating
Extraction and method for concentration have a clear superiority；
3）Raw material is used as using plumage awns chrysanthemum, lob grass, Blumea balsamifera, Le party Chinese prickly ash, leaves of Taxus mairei and Berberine hydrochloride
The insecticide of preparation, not only insecticidal activity is high, and can be dissolved in water, and processing method is simple, without using organic solvent, reduces
Environmental pollution；The insecticide by anti-plant pathogenic fungi and anti-plant disease pest experiment shows, with very strong killing fungi,
The effect of plant disease pest, to the plant pathogenic fungis such as rhizoctonia solani, Powdery Mildew and aphid, false eye leafhopper, diamondback moth,
Scale insect（Flower louse）Prevention effect Deng insect is good, using safe and reliable, can be used as the preventing and treating plant pest in gardens
Plant insecticide.
The percentage composition being related in present patent application is the weight percentage of pure material.
Embodiment
Below by way of specific embodiment, the invention will be further described, and the corresponding experiment of combination further illustrates the present invention
Beneficial effect.
Embodiment 1：By plumage awns chrysanthemum extract, the careless extract of lob, strangle party's pepper extract, leaves of Taxus mairei extraction
Thing, Berberine hydrochloride, Blumea balsamifera extraction of essential oil thing compare 15 according to weight:20:10: 10:2:5 ratio is mixed to get final production
Product.
Embodiment 2：By plumage awns chrysanthemum extract, the careless extract of lob, strangle party's pepper extract, leaves of Taxus mairei extraction
Thing, Berberine hydrochloride, Blumea balsamifera extraction of essential oil thing compare 10 according to weight:15:20: 5:1:4 ratio is mixed to get final production
Product.
Embodiment 3：By plumage awns chrysanthemum extract, the careless extract of lob, strangle party's pepper extract, leaves of Taxus mairei extraction
Thing, Berberine hydrochloride, Blumea balsamifera extraction of essential oil thing compare 20 according to weight:20:15: 15:2:3 ratio is mixed to get final production
Product.
Embodiment 4：By plumage awns chrysanthemum extract, the careless extract of lob, strangle party's pepper extract, leaves of Taxus mairei extraction
Thing, Berberine hydrochloride, Blumea balsamifera extraction of essential oil thing compare 13 according to weight:18:10: 15:1:2 ratio is mixed to get final production
Product.
Embodiment 5：Plumage awns chrysanthemum extract, the careless extract of lob, strangle party's pepper extract, southerm yew leaf extract,
Berberine hydrochloride, Blumea balsamifera extraction of essential oil thing compare 15 according to weight:15:15: 8:2:4 ratio is mixed to get final products.
Embodiment 6：By plumage awns chrysanthemum extract, the careless extract of lob, strangle party's pepper extract, leaves of Taxus mairei extraction
Thing, Berberine hydrochloride, Blumea balsamifera extraction of essential oil thing compare 20 according to weight:15:20: 10: 2:2 ratio is mixed to get finally
Product.
Described plumage awns chrysanthemum extract is prepared using following methods：Crushed after being dried is placed in container into the plumage awns chrysanthemum of coarse powder
It is interior, the distillation water boiling and extraction of addition plumage awns chrysanthemum 4-8 times of weight of coarse powder 1-2 times, each 0.5-1.5 hours, suction filtration discards filter
Slag, merges after extract solution, and obtained extract solution carries out flash concentration extremely under the conditions of 60-80 DEG C using Reduced Pressure Concentration Device
The 1/10-1/14 of original volume, dry powder is dried in vacuo into by concentrate again, produces plumage awns chrysanthemum extract dry powder, and plumage awns chrysanthemum carries
The yield for taking thing is 18.8%.
The careless extract of described lob is prepared using following methods：Careless crushed after being dried will be lobbed into coarse powder, according to lob
Careless coarse powder 1kg then ethanol consumption for 6-10 rise ratio add 50% EtOH Sonicate extract 2-3 times, it is each 30-50 minutes；
Suction filtration, discards filter residue, merges after extract solution, obtained extract solution is entered under the conditions of 60-70 DEG C using Reduced Pressure Concentration Device
Concentrate is spray-dried or is dried in vacuo into dry powder to the 1/10-1/14 of original volume, hung by row flash concentration
Ball grass extract dry powder, the yield of the careless extract of lob is 15.6%.
Described Le party pepper extract is prepared using following methods：Party's Chinese prickly ash crushed after being dried will be strangled into coarse powder, according to
Strangle party Chinese prickly ash coarse powder 1kg then ethanol consumption for 8-12 rise ratio add 95% EtOH Sonicate extract 2-3 times, each 20-40
Minute；Suction filtration, discards filter residue, merges after extract solution, and obtained extract solution concentrates dress under the conditions of 55-65 DEG C using vacuum film
Progress flash concentration is put to the 1/8-1/12 of original volume, concentrate is spray-dried or be dried in vacuo into dry powder, is obtained
To party's pepper extract dry powder is strangled, the yield for strangling party's pepper extract is 6.3%.
Described southerm yew leaf extract is prepared using following methods：By leaves of Taxus mairei crushed after being dried into thick
Powder, according to southerm yew leaf coarse powder 1kg then ethanol consumption for 6-10 rise ratio add 50% EtOH Sonicate extract 2-3
It is secondary, it is each 1-2 hours；Suction filtration, discards filter residue, merges after extract solution, and obtained extract solution uses vacuum under the conditions of 60-70 DEG C
Film enrichment facility carries out flash concentration to the 1/10-1/14 of original volume, is spray-dried or carried out vacuum by concentrate and do
Dry to obtain southerm yew leaf extract dry powder into dry powder, the yield of southerm yew leaf extract is 14.3%.
Described Blumea balsamifera extraction of essential oil thing is prepared using following methods：Crushed after being dried is put into the Blumea balsamifera of coarse powder
In flask, the distilled water of Blumea balsamifera 3 times of weight of coarse powder is added, volatile oil extractor is loaded onto, is carried using steam distillation formulation
Essential oil is taken, untill liquid clarification is slipped out, Blumea balsamifera extraction of essential oil thing is obtained, essential oil yield is 8.5%.
In use, by plumage awns chrysanthemum extract, the careless extract of lob, Blumea balsamifera extraction of essential oil thing, strangling party's pepper extract, south
Square Taxus leaf extract and Berberine hydrochloride can also add other liquid or auxiliary material system according to certain ratio mixed preparing
Into liquid preparation or emulsion, spraying is used.
Beneficial effects of the present invention are further illustrated below by way of corresponding experiment.
Experiment 1：Anti-fungal activity of plant pathogenic is determined
After the PDA prepared (potato, glucose, agar, distilled water) is sterilized, the PDA of solidification is added on super-clean bench
Heat is melted, and is imported in centrifuge tube, slant setting forms slant medium after solidification, standby.Sample is used using doubling dilution
Solvent is configured to various concentrations gradient.The sample configured is moved on the PDA surfaces in test tube with liquid-transfering gun, tried using inclined-plane
Tube method determines antifungal activity, and antibacterial power is judged according to minimum inhibitory concentration MIC value.Different samples are configured to solvent
250、125、62.5、31.25、15.625、7.813、3.906、1.953 μg·mL-1The dilution of 8 concentration gradients, uses highest
Concentration and least concentration carry out primary dcreening operation, then carry out dusting cover respectively one by one.The dosage such as the sample configured are put into the PDA in test tube
On surface, shake up, negative control group is only in PDA surfaces solubilizer, and blank group is not added with sample on PDA surfaces.By 8 kinds of different plants
Thing disease fungus is cultivated in culture dish, punched, then fungi is respectively put into the above-mentioned test tube for preparing sample solution, in culture
Cultivated in case after 48 h, according to whether long bacterium come continue adjust sample concentration.With the sample least concentration of not long bacterium as most
Low Mlc MIC.
With the antibacterial activity of slant tube method determination sample.Sample（Embodiment 1-6）To the minimum of 8 kinds of plant pathogenic fungis
The MIC value of Mlc is shown in Table 1.MIC value is smaller, and bacteriostatic activity is better.Illustrate that embodiment 1-6 has to 8 kinds of plant pathogenic fungis
Certain, stronger inhibitory action is especially respectively provided with to rhizoctonia solani, Powdery Mildew and hyphal cluster germ.
Experiment 2：Killing experiment to pest and disease damage（The insects such as aphid, false eye leafhopper, diamondback moth, scale insect）.
Table 2-5 result of the test shows：Under relatively low concentration, embodiment 1-6 is to aphid, false eye leafhopper, pickles
The agriculture and forestry injurious insects such as moth, scale insect have stronger prevention effect in the case where drug concentration is 0.5-g/L, show the product pair of the present invention
The insects such as aphid, false eye leafhopper, diamondback moth, scale insect have relatively strong killing action.
Experiment 3：Safety testing.
The safety testing of rat acute toxicity is carried out to embodiment 1-6, to evaluate the security of inventive samples.Experiment
Animal uses SD rats.Test procedure：Each 15 of male and female rat, totally 30 rats be randomly divided into 3 groups, every group 10 respectively.It is dynamic
After thing fasting overnight, according to the high, medium and low of rat body weight（0.5、1.5、2.5g/kg）Dosage group preparating liquid carries out gavage, sees
Examine and record rat poisoning symptom and death condition, the results are shown in Table 6.
Result of the test shows, after rat oral gavage, and obvious poisoning symptom do not occur in low dose group and middle dose group；High agent
Indivedual rats of amount group gradually started poisoning symptom occur after 1 hour, such as dispirited, loss of appetite, weight loss, but
Gradually recover after 1-2 weeks normal.Indivedual poisoners of high dose group are in death in 5 days after gavage contamination.Test result indicates that,
The product of the normal present invention is nontoxic to people and animals, uses safety.
Claims (7)
1. a kind of gardens plant insecticide, it is characterised in that by weight than following material composition：Plumage awns chrysanthemum extract,
The careless extract of lob, Le party pepper extract, southerm yew leaf extract, Berberine hydrochloride, Blumea balsamifera extraction of essential oil thing are pressed
Compare 10-20 according to weight:10-20:10-20: 5-15: 1-2:2-5 ratio mixing；
Described plumage awns chrysanthemum extract is prepared using following methods：Crushed after being dried is placed in container into the plumage awns chrysanthemum of coarse powder,
The distillation water boiling and extraction of addition plumage awns chrysanthemum 4-8 times of weight of coarse powder 1-2 times, each 0.5-1.5 hours, suction filtration discards filter residue, closes
And after extract solution, obtained extract solution carries out flash concentration to substance under the conditions of 60-80 DEG C using Reduced Pressure Concentration Device
Long-pending 1/10-1/14, dry powder is dried in vacuo into by concentrate again, produces plumage awns chrysanthemum extract dry powder；
The careless extract of described lob is prepared using following methods：The careless crushed after being dried that will lob is thick according to lob grass into coarse powder
Powder 1kg then ethanol consumption for 6-10 rise ratio add 50% EtOH Sonicate extract 2-3 times, it is each 30-50 minutes；Suction filtration,
Filter residue is discarded, is merged after extract solution, obtained extract solution is flashed under the conditions of 60-70 DEG C using Reduced Pressure Concentration Device
The 1/10-1/14 of original volume is concentrated into, concentrate is spray-dried or be dried in vacuo into dry powder, lob grass is obtained and carries
Take thing dry powder；
Described Le party pepper extract is prepared using following methods：Party's Chinese prickly ash crushed after being dried will be strangled into coarse powder, according to Le party
Chinese prickly ash coarse powder 1kg then ethanol consumption for 8-12 rise ratio add 95% EtOH Sonicate extract 2-3 time, each 20-40 divide
Clock；Suction filtration, discards filter residue, merges after extract solution, and obtained extract solution uses Reduced Pressure Concentration Device under the conditions of 55-65 DEG C
Flash concentration is carried out to the 1/8-1/12 of original volume, concentrate is spray-dried or be dried in vacuo into dry powder, is obtained
Strangle party's pepper extract dry powder；
Described southerm yew leaf extract is prepared using following methods：By leaves of Taxus mairei crushed after being dried into coarse powder,
According to southerm yew leaf coarse powder 1kg then ethanol consumption for 6-10 rise ratio add 50% EtOH Sonicate extract 2-3 times,
1-2 hours every time；Suction filtration, discards filter residue, merges after extract solution, and obtained extract solution is thin using vacuum under the conditions of 60-70 DEG C
Film condensing device carries out flash concentration to the 1/10-1/14 of original volume, and concentrate is spray-dried or is dried in vacuo
Into dry powder, southerm yew leaf extract dry powder is obtained；
Described Blumea balsamifera extraction of essential oil thing is prepared using following methods：Crushed after being dried is put into flask into the Blumea balsamifera of coarse powder
In, the distilled water of Blumea balsamifera 3 times of weight of coarse powder is added, volatile oil extractor is loaded onto, essence is extracted using steam distillation formulation
Oil, untill liquid clarification is slipped out, obtains Blumea balsamifera extraction of essential oil thing.
2. a kind of gardens plant insecticide as claimed in claim 1, it is characterised in that plumage awns chrysanthemum extract, lob grass
Extract, Le party pepper extract, southerm yew leaf extract, Berberine hydrochloride, the weight ratio of Blumea balsamifera extraction of essential oil thing
For 12-18:12-18:12-18:6-12: 1.2-1.8:2.5-4.5.
3. a kind of gardens plant insecticide as claimed in claim 1, it is characterised in that plumage awns chrysanthemum extract, lob grass
Extract, Le party pepper extract, southerm yew leaf extract, Berberine hydrochloride, the weight ratio of Blumea balsamifera extraction of essential oil thing
For 14-16:14-16:14-16:8-10: 1.4-1.6:3-4.
4. a kind of gardens plant insecticide as claimed in claim 1, it is characterised in that in described plumage awns chrysanthemum extract：
The decocting time added after distilled water is each 0.5-1.5 hours；Extract solution is 65-75 DEG C in flash concentration temperature, is concentrated into original
The 1/11-1/13 of volume.
5. a kind of gardens plant insecticide as claimed in claim 1, it is characterised in that in the careless extract of described lob：
The ultrasonic extraction time is 35-45 minutes every time, and flash concentration temperature is 62-65 DEG C, is concentrated into the 1/11-1/13 of original volume.
6. a kind of gardens plant insecticide as claimed in claim 1, it is characterised in that described Le party pepper extract
In：The ultrasonic extraction time is each 20-40 minutes, and flash concentration temperature is 58-60 DEG C, is concentrated into the 1/9-1/11 of original volume.
7. a kind of gardens plant insecticide as claimed in claim 1, it is characterised in that described leaves of Taxus mairei is carried
Take in thing：The ultrasonic extraction time is each 1.2-1.5 hours；Flash concentration temperature is 62-65 DEG C, is concentrated into the 1/11- of original volume
1/13。
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| CN105165912B (en) * | 2015-08-26 | 2018-02-27 | 湖州旭龙生物化学有限公司 | A kind of bactericidal composition containing thiophanate-methyl and careless extract of lobbing |
| CN109384529A (en) * | 2017-08-08 | 2019-02-26 | 贵州美人芋农业发展有限公司 | A kind of preparation method of greenery element |
| CN107372657B (en) * | 2017-08-10 | 2020-03-31 | 黑龙江省农业科学院经济作物研究所 | Plant-derived pesticide and preparation method thereof |
| CN107691066A (en) * | 2017-08-26 | 2018-02-16 | 普定县和权茶叶专业合作社 | A kind of implantation methods of tealeaves |
| CN107410380A (en) * | 2017-09-18 | 2017-12-01 | 何玉莲 | A kind of ornamental plant flowers High-efficiency insecticidal pesticide reagent and preparation method thereof |
| CN108294058A (en) * | 2018-03-29 | 2018-07-20 | 合肥卓畅农业科技有限公司 | A kind of safety and environmental protection effective, harmless slow-release pesticidal particle and preparation method thereof |
| CN108575671A (en) * | 2018-04-11 | 2018-09-28 | 合肥卓畅农业科技有限公司 | A kind of insect prevention organic nutrient soil of safety and environmental protection |
| CN111802411B (en) * | 2020-07-13 | 2021-11-30 | 安徽省农业科学院茶叶研究所 | Pharmaceutical composition for preventing and treating plant diseases and insect pests in seedling stage of organic tea garden |
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