CN110074145A - Application of the Lycoris aurea ethanol extract on pesticide - Google Patents
Application of the Lycoris aurea ethanol extract on pesticide Download PDFInfo
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- CN110074145A CN110074145A CN201810099363.3A CN201810099363A CN110074145A CN 110074145 A CN110074145 A CN 110074145A CN 201810099363 A CN201810099363 A CN 201810099363A CN 110074145 A CN110074145 A CN 110074145A
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- lycoris aurea
- ethanol extract
- total alkali
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The present invention relates to Lycoris aurea (Lycoris aurea (L ' Herit.) Herb) application of the bulb (ethanol extract and biology total alkali) on pesticide.This ethanol extract and biology total alkali are used as plant virus agent, can inhibit tobacco mosaic virus (TMV) well；This ethanol extract and biology total alkali are used as fungicide, and to tomato early epidemic, gibberella saubinetii, potato late blight, Phytophthora capsici, rape sclerotium, cucumber grey mold, rice banded sclerotial blight, cucumber be withered, peanut foxiness, apple wheel line, wheat line are withered, corn stigma, watermelon anthrax, rice bakanae disease have inhibitory activity.This ethanol extract and biology total alkali, which are used as insecticide, has cytotoxicity to mythimna separata, mosquito larvae, bollworm, corn borer and diamondback moth.Lycoris aurea bulb ethanol extract and Lycoris aurea bulb biology total alkali of the invention can be used for diseases and pests of agronomic crop green prevention and control.
Description
Technical field
The present invention relates to application of the Lycoris aurea ethanol extract on pesticide, including ethanol extract and Lycoris aurea biology are always
Alkali, application are related to the application of Antiphytoviral, the application of sterilization, and the application of desinsection belongs to technical field of pesticide.
Background technique
World population sustainable growth, staple food supply problem become important topic urgently to be resolved.Pesticide is in increases in grain production
It played an important role, but the long-term unreasonable use of traditional chemical synthetic pesticide has seriously affected ecological environment (pesticide
1999,38 (10), 9-10).With the development of the society, the food safety and ecological protection consciousness of people are continuously improved, pesticide phase
The requirement for closing laws and regulations is also increasingly strict, this makes environmentally friendly agricultural chemical have become the hot spot that people study, wherein planting
Material resource pesticide, which becomes, studies preferred (J.Agric.Food Chem.2014,62 (41), 9987-9999).Lycoris aurea (Lycoris
Aurea (L ' Herit.) Herb) also known as Lycoris, iron color arrow, big adder tongue herb, it is Amaryllidaceae Lycoris representativeness plant, suddenly
Ground is laughed at as traditional Chinese medicine for eliminating the phlegm, emetic, is also used as soil pesticide by peasant, is that a kind of popular ornamental ornamental value is planted now
Object (Chinese Plants will).
CN101837075A, which is disclosed, is related to Lycoris aurea extract or organic solvent extracts for preventing or treating dementia
The purposes of disease.
CN105199872A discloses a kind of dish washing liquid of Chinese medicine liquid containing 69-88.5%, contains iron in Chinese medicine liquid
3-10 parts of color arrow.
CN105238582A discloses a kind of Antibacterial laundry detergent of Chinese medicine liquid containing 69-88.5%, contains in Chinese medicine liquid
There is 3-10 parts of iron color arrow.
CN106165707A discloses a kind of insecticide containing 10-15% Chinese medicine extract applied on pomegranate tree,
Wherein contain 0.2-0.6 parts of golden lycoris bulbs in Chinese medicine extract.
CN106035417A discloses a kind of insecticide for preventing and treating pimento yellow tea mite pest, wherein containing Lycoris aurea 1.8-
2.0 part.
Summary of the invention
It is an object of the present invention to provide application of the Lycoris aurea ethanol extract on pesticide, including ethanol extract and Lycoris aurea
Two kinds of biology total alkali combinations, application are related to Antiphytoviral, sterilize, the application of desinsection.
Lycoris aurea ethanol extract of the present invention and Lycoris aurea biology total alkali extracting method are as follows: take it is fresh suddenly
Bulb (being purchased from Jiangsu Province, flowers market, Suqian) 1.49kg is laughed at, extracts 7 days in the ethyl alcohol of 2.5L after shredding, then filters, is received
Collect filtrate.Filter residue continues one step process as above and extracts and be repeated twice or more times.All filtrates are collected and are concentrated in vacuo, final
It is Lycoris aurea bulb ethanol extract to black paste or pulpous state.Lycoris aurea bulb ethanol extract 10g is taken, it is dilute that 10mL is added
Hydrochloric acid (1mol/L), ultrasonic echography extract (40mL) with chloroform after twenty minutes.Resulting water phase adjusts pH to 10 with ammonium hydroxide, so
It is extracted afterwards with chloroform (30mL × 3).After merging organic phase and being dried with anhydrous sodium sulfate, the yellow solid being concentrated to get is to neglect
Laugh at bulb alcohol biological total alkali in ground.
Lycoris aurea ethanol extract and Lycoris aurea biology total alkali provided by the invention have preferable anti-phytoviral activity, kill
Bacterium and insecticidal activity.
Lycoris aurea biology total alkali provided by the invention exists to the inhibiting rate of tobacco mosaic virus (TMV) under the concentration of 500mg/L
40% or so, it is suitable in commercially available antivirotic virazole.
Lycoris aurea ethanol extract and Lycoris aurea biology total alkali provided by the invention are to tomato early epidemic, gibberella saubinetii, Ma Ling
Potato late blight, Phytophthora capsici, rape sclerotium, cucumber grey mold, rice banded sclerotial blight, cucumber be withered, peanut foxiness, apple wheel line, rice line
Withered, corn stigma, watermelon anthrax, rice bakanae disease have inhibiting effect.
Lycoris aurea ethanol extract and Lycoris aurea biology total alkali provided by the invention are to mythimna separata, mosquito larvae, bollworm, corn
Snout moth's larva, aphid, adult mite and diamondback moth have cytotoxicity.
Lycoris aurea ethanol extract and Lycoris aurea biology total alkali provided by the invention can be used directly as pesticide, can be added
On the carrier that agriculturally receives use, can also be used in compounding.
Specific embodiment
Following embodiments and biological test result can be used to further illustrate the present invention, but be not intended to limit this hair
It is bright.
Embodiment 1: resisting tobacco mosaic virus:
Mensuration program is as follows
Virus purification and concentration mensuration: Virus purification and concentration mensuration give birth to referring to Nankai University's element and survey room establishment tobacco
Mosaic virus SOP regulation enforcement.Viral crude extract measures concentration after 2 polyethylene glycol centrifugal treatings, and 4 DEG C of refrigerations are spare.
Compound solution is prepared: after weighing, DMF dissolution is added in raw medicine, is made 1 × 10-5μ g/mL mother liquor, it is rear to be spat with containing 1%
Warm 80 aqueous solutions are diluted to required concentration；
Living body protective effect: the uniform western cigarette of 3-5 leaf phase coral of growing way, complete stool spray pesticide, 3 weights of every processing are selected
It is multiple, and set Tween 80 aqueous solution control.After for 24 hours, blade face dispenses diamond dust (500 mesh), virus liquid is dipped with writing brush, in full leaf face
It is dabbed 2 times along offshoot direction, palm support is used below blade, 10 μ g/mL of virus concentration is rinsed after inoculation with flowing water.3 days postscripts
Scab number is recorded, preventive effect is calculated.
Living body therapeutic effect: selecting the uniform western cigarette of 3-5 leaf phase coral of growing way, and with writing brush full leaf virus inoculation, virus is dense
Degree is 10 μ g/mL, is rinsed after inoculation with flowing water.After blade face receipts are dry, complete stool spray pesticide, 3 repetitions of every processing, and set 1 ‰ and spit
Warm 80 aqueous solutions control.After 3 days, scab number is recorded, calculates preventive effect.
Living body passivation: selecting the uniform western cigarette of 3-5 leaf phase coral of growing way, and medicament and isometric viral juice are mixed
After closing passivation 30min, frictional inoculation, 20 μ g/mL of virus concentration is rinsed with flowing water after inoculation, is repeated 3 times, if 1 ‰ Tween 80s
Aqueous solution control.Number scab number, calculated result after 3 days.Inhibiting rate (%)=[(control withered spot number-processing withered spot number)/control is withered
Spot number] × 100%
Test result is as follows for the activity of resisting tobacco mosaic virus of Lycoris aurea ethanol extract and Lycoris aurea biology total alkali:
1 Lycoris aurea ethanol extract of table and Lycoris aurea biology total alkali resisting tobacco mosaic virus (TMV) activity
Resisting tobacco mosaic virus of the Lycoris aurea biology total alkali under 500mg/L and 100mg/L concentration is living as can be seen from Table 1
Property and commercially available antivirotic virazole activity quite.
Embodiment 2: Fungicidally active:
Mensuration program is as follows
By taking tomato early blight bacterium as an example, other bacterium can be changed into
In vitro test method: tomato early blight bacterium is connected in PDA culture medium and is cultivated 7 days, with punch in colony edge
The bacterium dish for producing diameter 4cm, which is inoculated into the PDA culture medium containing 50 μ g/mL and without medicament, to be cultivated 4 days, and it is straight to measure bacterium colony
Diameter, and compares the inhibition percentage for calculating medicament.
Test result is as follows for the bactericidal activity of Lycoris aurea ethanol extract and Lycoris aurea biology total alkali:
The bactericidal activity of table 2 Lycoris aurea ethanol extract and Lycoris aurea biology total alkali
The bactericidal activity (Continued) of table 2 Lycoris aurea ethanol extract and Lycoris aurea biology total alkali
Lycoris aurea ethanol extract and Lycoris aurea biology total alkali are in 50mg/L to tomato early epidemic, wheat as can be seen from Table 2
Red mould, potato late blight, Phytophthora capsici, rape sclerotium, cucumber grey mold, rice banded sclerotial blight, cucumber be withered, peanut foxiness, apple wheel
Line, wheat line be withered, corn stigma, watermelon anthrax, rice bakanae disease have inhibiting effect, and wherein Lycoris aurea biology total alkali is in 50 μ g/
Inhibiting rate mL withered to wheat line reaches 75.6%.
Embodiment 3: insecticidal activity
The active testing of bollworm
The experimental method of bollworm: leaf dipping method.After concentration needed for configuring, the blade that diameter is about 5~6cm is immersed medical fluid
In 5~6 seconds, take out, be placed on blotting paper and dry, be placed in specified culture dish, access 10 3 instar larvaes, be put into 27 ± 1 DEG C
Insectary in observe 3~4 days after inspection result.
The active testing of mythimna separata
The experimental method of mythimna separata: leaf dipping method.After concentration needed for configuring, the blade leaching for being about 5~6cm diameter is into the liquid
It 5~6 seconds, takes out, has been placed on blotting paper and has done, be placed in specified culture dish, access 10 3 instar larvaes, be put into 27 ± 1 DEG C
Inspection result after being observed 3~4 days in insectary.
The active testing of corn borer
The experimental method of corn borer: leaf dipping method is tried, after postponing required concentration, the blade that diameter is about 5~6cm is immersed
It 5~6 seconds in medical fluid, takes out, has been placed on blotting paper and has done, be placed in specified culture dish, access 10 3 instar larvaes, be put into 27
Inspection result after being observed 3~4 days in ± 1 DEG C of insectary.
The active testing of mosquito larvae
The experimental method of mosquito larvae: culex pipiens pallens, the feeding normal population of indoor word.Weigh test compound about
5mg adds 5mL acetone (or suitable solvent) in penicillin medicine bottle, oscillation dissolution, as 1000 μ g/mL mother liquors.Pipette 1mL
Mother liquor is added in the 100mL beaker for filling 89.9mL water, and larvae at the beginning of choosing 10 4 ages falls together together with 10mL feeding liquid
Enter in beaker, the concentration of medical fluid is 10 μ g/mL.It is put into standard process chamber, for 24 hours inspection result.To be tested containing 0.5mL
The aqueous solution of solvent is blank control.
Diamondback moth larvae active testing
The leaf dipping method proposed using international resistance Action Committee (IRAC).Weighed on assay balance 6mg medicine sample in
In 10mL small beaker, adds 50 μ L dimethylformamides (analysis is pure) dissolution, add 10mL water that 600 μ g/mL medical fluids are made.With straight peen eye
Section's tweezers impregnate cabbage leaves, the time 2~3 seconds, get rid of extraction raffinate.1 tablet once, and totally 3, each sample.By sample flag sequence according to
It is secondary to be placed on processing paper.It after medical fluid is dry, is put into the straight type pipe for having markd 10cm long, accesses 2 age diamondback moth larvaes, use
Gauze covers nozzle.Experiment process is placed in standard process chamber, inspection result after 96h.Each compound is repeated 3 times.Control
Only to Guan Shuizhong addition emulsifier and solvent is steamed, stir evenly.
Lycoris aurea ethanol extract and Lycoris aurea biology total alkali are to bollworm, corn borer, mythimna separata, diamondback moth, mosquito larvae poison
It is as follows to kill active testing result:
The test worm death rate (%) under the conditions of various concentration (μ g/mL) of table 3
Lycoris aurea ethanol extract and Lycoris aurea biology total alkali are to bollworm as can be seen from Table 3, corn borer, and mythimna separata is small
Diamond-back moth, mosquito larvae play the role of killing, and wherein Lycoris aurea biology total alkali is living to the killing of diamondback moth under 600 μ g/mL concentration
Property is suitable with matrine.
Embodiment 4: diamondback moth field efficacy test
Field plot experiment is implemented by Tianjin Plant Protection Institute in October, 2017.The ethyl alcohol of configuration 10% extracts
Object missible oil: 10g Lycoris aurea ethanol extract, 73g DMF, 7g tensiofix (B8426), 7g B7453,3g SK-44.Using
Sprayer carries out the page and is sprayed, plot area: 25 square metres, 1 natural furrow, in triplicate.Testing blank control is that sprinkling is clear
Water, matrine are positive control.
4 Lycoris aurea ethanol extract of table kills diamondback moth field plot experimental result
10% missible oil for utilizing Lycoris aurea ethanol extract as can be seen from Table 4, has after being administered 2 days under three concentration
Effect, effect enhances after 7 days, but effect declines after 10 days.With concentration (100,200,300g/hm2) enhancing imitate
Enhancing, but 200g/hm2And 300g/hm2The preventive effect enhancing after application 7 days of concentration is unobvious.
Claims (8)
1. application of the Lycoris aurea bulb ethanol extract on pesticide, it is characterised in that ethanol extract includes Lycoris aurea bulb second
Alcohol extracting thing and two kinds of Lycoris aurea bulb biology total alkali combinations, the extract can be applied to diseases and pests of agronomic crop green prevention and control.
2. the application of Lycoris aurea bulb ethanol extract described in claim 1 and Lycoris aurea bulb biology total alkali on pesticide,
It is characterized in that wherein the Lycoris aurea bulb ethanol extract is prepared by the following method: taking fresh Lycoris aurea bulb
1.49kg is extracted 7 days in the ethyl alcohol of 2.5L after shredding, is then filtered, and collects filtrate, and filter residue continues one step process extraction as above
And be repeated twice or more times, all filtrates are collected and are concentrated in vacuo, black paste is finally obtained or pulpous state is Lycoris aurea bulb
Ethanol extract.
3. the application of Lycoris aurea bulb ethanol extract described in claim 1 and Lycoris aurea bulb biology total alkali on pesticide,
It is characterized in that wherein the Lycoris aurea bulb alcohol biological total alkali crosses following methods preparation: taking according to described in claim 2
Acquisition Lycoris aurea bulb ethanol extract 10g, be added 10mL dilute hydrochloric acid (1mol/L), ultrasonic echography uses chlorine after twenty minutes
Imitative extraction (40mL) is extracted, merging extracts institute three times after resulting water phase ammonium hydroxide adjusting pH is 10 with chloroform (30mL × 3)
Organic phase (90mL) and dry with anhydrous sodium sulfate, after filtering sodium sulphate, being concentrated to get yellow solid is Lycoris aurea ball
Stem biology total alkali.
4. the application of Lycoris aurea bulb ethanol extract described in claim 1 and Lycoris aurea bulb biology total alkali on pesticide,
It is characterized in that its anti-phytoviral activity, can inhibit tobacco mosaic virus (TMV) well.
5. the application of Lycoris aurea bulb ethanol extract described in claim 1 and Lycoris aurea bulb biology total alkali on pesticide,
It is characterized in that it is as fungicide to tomato early epidemic, gibberella saubinetii, potato late blight, Phytophthora capsici, rape sclerotium, cucumber ash
Mould, rice banded sclerotial blight, cucumber be withered, peanut foxiness, apple wheel line, wheat line are withered, corn stigma, watermelon anthrax, rice bakanae disease all
There is good inhibitory activity.
6. the application of Lycoris aurea bulb ethanol extract described in claim 1 and Lycoris aurea bulb biology total alkali on pesticide,
It is characterized in that it has cytotoxicity as insecticide, to mythimna separata, mosquito larvae, bollworm, corn borer and diamondback moth.
7. the application of Lycoris aurea bulb ethanol extract described in claim 1 and Lycoris aurea bulb biology total alkali on pesticide,
Its feature can be respectively as being directly used in agricultural disease in Lycoris aurea bulb ethanol extract and Lycoris aurea bulb biology total alkali
Prevention and treatment.
8. the application of Lycoris aurea bulb ethanol extract described in claim 1 and Lycoris aurea bulb biology total alkali on pesticide,
Its feature can be made compounding agent with other pesticides in Lycoris aurea bulb ethanol extract and Lycoris aurea bulb biology total alkali and be used for
Agricultural disease prevention and treatment.
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| CN201810099363.3A CN110074145B (en) | 2018-01-26 | 2018-01-26 | Application of michelia figo ethanol extract in pesticide |
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| CN201810099363.3A CN110074145B (en) | 2018-01-26 | 2018-01-26 | Application of michelia figo ethanol extract in pesticide |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613257A (en) * | 2012-03-05 | 2012-08-01 | 杭州绿普达生物科技有限公司 | Lycoris radiata bulb extractive pesticide and method for preparing extractive |
| US20120244095A1 (en) * | 2011-03-23 | 2012-09-27 | Basf Se | Compositions containing polymeric, ionic compounds comprising imidazolium groups |
| EP2609929A1 (en) * | 2010-08-27 | 2013-07-03 | Korea Institute of Science and Technology | Composition for preventing or treating dementia |
| CN105622686A (en) * | 2016-01-11 | 2016-06-01 | 江苏省中国科学院植物研究所 | Alkaloid compound and preparation method as well as application thereof |
| CN106797965A (en) * | 2016-12-19 | 2017-06-06 | 湖北工程学院 | Application of the short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine |
| CN107258799A (en) * | 2016-04-06 | 2017-10-20 | 南开大学 | The application of lycorine (lycorine) and lycorine hydrochloride on agricultural chemicals |
-
2018
- 2018-01-26 CN CN201810099363.3A patent/CN110074145B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2609929A1 (en) * | 2010-08-27 | 2013-07-03 | Korea Institute of Science and Technology | Composition for preventing or treating dementia |
| US20120244095A1 (en) * | 2011-03-23 | 2012-09-27 | Basf Se | Compositions containing polymeric, ionic compounds comprising imidazolium groups |
| CN102613257A (en) * | 2012-03-05 | 2012-08-01 | 杭州绿普达生物科技有限公司 | Lycoris radiata bulb extractive pesticide and method for preparing extractive |
| CN105622686A (en) * | 2016-01-11 | 2016-06-01 | 江苏省中国科学院植物研究所 | Alkaloid compound and preparation method as well as application thereof |
| CN107258799A (en) * | 2016-04-06 | 2017-10-20 | 南开大学 | The application of lycorine (lycorine) and lycorine hydrochloride on agricultural chemicals |
| CN106797965A (en) * | 2016-12-19 | 2017-06-06 | 湖北工程学院 | Application of the short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine |
Non-Patent Citations (3)
| Title |
|---|
| 任伟: "石蒜碱和小檗碱对植物病原真菌抑制作用及其抑菌生理指标分析", 《中国优秀硕士学位论文全文数据库（电子期刊）农业科技辑》 * |
| 孙志峰 等: "黔产药用植物忽地笑中的化学成分研究", 《北方药学》 * |
| 尹莲: "《天然药物化学》", 31 July 2017, 中国中医药出版社 * |
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