CN111602686B - Pharmaceutical composition for preventing peanut root rot based on limonene and preparation method thereof - Google Patents

Pharmaceutical composition for preventing peanut root rot based on limonene and preparation method thereof Download PDF

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CN111602686B
CN111602686B CN202010540721.7A CN202010540721A CN111602686B CN 111602686 B CN111602686 B CN 111602686B CN 202010540721 A CN202010540721 A CN 202010540721A CN 111602686 B CN111602686 B CN 111602686B
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extract
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pharmaceutical composition
limonene
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CN111602686A (en
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于建垒
刘同金
李瑞娟
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Institute of Plant Protection Shandong Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a limonene-based pharmaceutical composition for preventing peanut root rot and a preparation method thereof. The pharmaceutical composition comprises the following raw materials in parts by weight: 2-3 parts of limonene, 3-5 parts of Chinese honeylocust fruit extract, 8-10 parts of garlic extract, 0.1-0.12 part of zinc chloride, 1-2 parts of cetearyl glucoside and 0.6-0.8 part of sodium alginate. The pharmaceutical composition prepared by the invention has good suspension property, can form uniform suspension, and does not delaminate or precipitate; the pharmaceutical composition prepared by the invention can effectively prevent peanut root rot, and improve the yield and quality of peanuts.

Description

Pharmaceutical composition for preventing peanut root rot based on limonene and preparation method thereof
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a limonene-based pharmaceutical composition for preventing peanut root rot and a preparation method thereof.
Background
Peanuts are common oil crops in the north, and as a root growth crop, the quality of the root usually determines the yield. Root rot can occur in various growth periods of peanuts. Infection before emergence of seedlings can cause seed rot and bud rot; the disease infection in the seedling stage causes the root system of the seedling to rot and the seedling to wither; after the plants are grown, the plants are caused to have root rot, stem base rot and pod rot. The aerial parts of the diseased plants are mainly short, poor in growth, yellow in leaves and finally withered. The peanut root rot belongs to soil-borne diseases, and germs can infect crops or spread seeds when the disease residues live through the winter in the soil to the next year. At present, the peanuts are used for preventing diseases in time after seedling alignment, the spraying or drenching method is usually adopted for applying the pesticides, the triazolone and the carbendazim are selected for multiple purposes, the pesticide application amount is large, the pesticide residue is easy to cause, and the pesticide is not favorable for the environment and the health of human bodies. The research and development of a high-efficiency and low-residue medicine becomes an urgent problem to be solved.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a pharmaceutical composition for preventing peanut root rot based on limonene.
The invention also provides a preparation method of the pharmaceutical composition.
The technical scheme adopted by the invention for realizing the purpose is as follows:
the invention provides a pharmaceutical composition for preventing peanut root rot based on limonene, which comprises the following raw materials in parts by weight: 2-3 parts of limonene, 3-5 parts of Chinese honeylocust fruit extract, 8-10 parts of garlic extract, 0.1-0.12 part of zinc chloride, 1-2 parts of cetearyl glucoside and 0.6-0.8 part of sodium alginate.
The saponin extract used in the invention is prepared by the following method: grinding fructus Gleditsiae Abnormalis into fine powder, adding 25% ethanol water solution, reflux extracting at 40-50 deg.C for 60min, filtering, and concentrating to half of original volume.
The feed-liquid ratio of the saponin to the ethanol aqueous solution is 1 g: 10 mL.
The garlic extract used in the invention is prepared by the following method: grinding Bulbus Allii, adding 50% acetic acid water solution, reflux extracting at 35-40 deg.C for 80min, filtering, and concentrating to half of original volume.
The feed-liquid ratio of the garlic to the acetic acid aqueous solution is 1 g: 10 mL.
The invention also provides a preparation method of the pharmaceutical composition, which comprises the following steps:
(1) mixing fructus Gleditsiae Abnormalis extract and Bulbus Allii extract, adding zinc chloride, heating to 35-40 deg.C, stirring for 1-1.5 hr to obtain extract mixture;
(2) adding limonene, cetearyl glucoside and sodium alginate into the extract mixture, stirring, adding diacetyl tartaric acid monoglyceride and diglyceride, and spray drying.
Furthermore, the adding amount of the diacetyl tartaric acid ester of mono-and diglycerides accounts for 0.12% of the weight of the extract mixed liquor.
Further, the spray drying conditions are that the inlet temperature is 90-100 ℃, the outlet temperature is 80-90 ℃, and the feeding speed is 40 ml/min.
The invention has the beneficial effects that:
(1) the pharmaceutical composition prepared by the invention has good suspension property, can form uniform suspension, and does not delaminate or precipitate;
(2) the pharmaceutical composition prepared by the invention can effectively prevent peanut root rot, and improve the yield and quality of peanuts.
Detailed Description
The technical solution of the present invention is further explained and illustrated by the following specific examples.
Example 1
The pharmaceutical composition comprises the following components: 2 parts of limonene, 5 parts of Chinese honeylocust fruit extract, 8 parts of garlic extract, 0.1 part of zinc chloride, 1.5 parts of cetearyl glucoside and 0.7 part of sodium alginate.
The saponin extract is prepared by the following method: grinding the Chinese honeylocust into fine powder, and then mixing the fine powder with the raw materials according to a material-liquid ratio of 1 g: adding 10mL of ethanol water with the volume fraction of 25%, refluxing and extracting at 40-50 ℃ for 60min, filtering, and concentrating to half of the original volume.
The garlic extract is prepared by the following method: grinding garlic, and then mixing the ground garlic and the ground garlic according to a feed-liquid ratio of 1 g: adding 50% acetic acid aqueous solution into 10mL, reflux extracting at 35-40 deg.C for 80min, filtering, and concentrating to half of original volume.
The preparation method comprises the following steps:
(1) mixing the saponin extract and the garlic extract in parts by weight, adding zinc chloride, heating to 35-40 ℃, and stirring for 1.5 hours at the constant temperature to obtain an extract mixed solution;
(2) adding limonene, cetearyl glucoside and sodium alginate into the extract mixture, stirring, adding diacetyl tartaric acid monoglyceride and diglyceride 0.12 wt% of the extract mixture, and spray drying.
Example 2
The pharmaceutical composition comprises the following components: 2 parts of limonene, 4 parts of Chinese honeylocust fruit extract, 10 parts of garlic extract, 0.12 part of zinc chloride, 1.5 parts of cetearyl glucoside and 0.8 part of sodium alginate.
The preparation method is the same as example 1.
Example 3
The pharmaceutical composition comprises the following components: 2.5 parts of limonene, 5 parts of Chinese honeylocust fruit extract, 9 parts of garlic extract, 0.12 part of zinc chloride, 1.5 parts of cetearyl glucoside and 0.5 part of sodium alginate.
The preparation method is the same as example 1.
Comparative example 1
The composition of the pharmaceutical composition was the same as in example 2.
The preparation method comprises the following steps:
(1) the raw materials in parts by weight are uniformly mixed and then spray-dried.
Comparative example 2
The composition of the pharmaceutical composition was the same as in example 2.
The saponin extract was as in example 1.
The garlic extract is prepared by the following method: grinding garlic, and then mixing the ground garlic and the ground garlic according to a feed-liquid ratio of 1 g: adding 10mL of ethanol water solution with the volume fraction of 25%, refluxing and extracting at 35-40 ℃ for 80min, filtering, and concentrating to half of the original volume.
The preparation method is the same as example 1.
Comparative example 3
The composition of the pharmaceutical composition was the same as in example 2.
The saponin extract is prepared by the following method: grinding the Chinese honeylocust into fine powder, and then mixing the fine powder with the raw materials according to a material-liquid ratio of 1 g: adding 50% acetic acid aqueous solution into 10mL, reflux extracting at 40-50 deg.C for 60min, filtering, and concentrating to half of original volume.
The garlic extract was extracted in the same manner as in example 1.
The preparation method is the same as example 1.
Effects of the embodiment
Selecting Xiujin county in Texas as a test base, wherein the peanut variety is No. 25, the test base is a land plot of 2 years of continuous cropping, dividing the test field into 30 plots, repeating 3 groups in each embodiment comparative example, and simultaneously setting a control group, wherein the control group 1 is not added with limonene and the other embodiments are the same as the embodiment 2; control group 2 was prepared by replacing garlic extract with the same amount of garlic extract without adding saponin extract, and the other examples were the same as example 2; the control group 3 was prepared by replacing the garlic extract with the same amount of saponin extract, and the same procedure as in example 2 was repeated; the specific application method comprises the following steps: preparing 100 times of liquid after planting peanuts, spraying the surface of the peanuts, preparing 80 times of liquid before flowering and in a next-needle period, spraying plants, wherein the liquid can be sprayed to the base of a stem, and meanwhile, a blank control group is arranged, so that no treatment is carried out, and other field management is the same; counting the disease strain rate of the peanut with root rot, calculating the incidence rate of the root rot, and calculating the control effect by adopting the incidence rate, wherein the control effect = [ incidence rate of a blank control area-incidence rate of a treatment area ]/incidence rate of the blank control area, and the specific results are shown in table 1.
TABLE 1
Figure DEST_PATH_IMAGE002
And (II) counting the yield of the plots (pod yield), wherein the specific results are shown in Table 2.
TABLE 2
Figure DEST_PATH_IMAGE004
Thirdly, the medicinal compositions prepared in examples 1-3 and comparative examples 1-3 are subjected to suspension rate statistics according to GB/T14825-93, and specific results are shown in Table 3.
TABLE 3
Figure DEST_PATH_IMAGE006

Claims (5)

1. The preparation method of the pharmaceutical composition for preventing peanut root rot based on limonene is characterized in that the pharmaceutical composition comprises the following raw materials in parts by weight: 2-3 parts of limonene, 3-5 parts of Chinese honeylocust fruit extract, 8-10 parts of garlic extract, 0.1-0.12 part of zinc chloride, 1-2 parts of cetearyl glucoside and 0.6-0.8 part of sodium alginate;
the method specifically comprises the following steps:
(1) mixing fructus Gleditsiae Abnormalis extract and Bulbus Allii extract, adding zinc chloride, heating to 35-40 deg.C, stirring for 1-1.5 hr to obtain extract mixture;
(2) adding limonene, cetearyl glucoside and sodium alginate into the extract mixed solution, stirring, adding diacetyl tartaric acid monoglyceride and diglyceride, and spray drying;
the saponin extract is prepared by the following method: grinding fructus Gleditsiae Abnormalis into fine powder, adding 25% ethanol water solution, reflux extracting at 40-50 deg.C for 60min, filtering, and concentrating to half of original volume;
the garlic extract is prepared by the following method: grinding Bulbus Allii, adding 50% acetic acid water solution, reflux extracting at 35-40 deg.C for 80min, filtering, and concentrating to half of original volume.
2. The production method according to claim 1, wherein the feed-to-liquid ratio of the saponin and the aqueous ethanol solution is 1 g: 10 mL.
3. The preparation method according to claim 1, wherein the feed-liquid ratio of the garlic to the acetic acid aqueous solution is 1 g: 10 mL.
4. The method according to claim 1, wherein the diacetyl tartaric acid esters of monoglycerides and diglycerides is added in an amount of 0.12% by weight of the extract mixture.
5. The method according to claim 1 or 2, wherein the spray drying is performed under conditions of an inlet temperature of 90 to 100 ℃, an outlet temperature of 80 to 90 ℃, and a feed rate of 40 ml/min.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015199342A1 (en) * 2014-06-26 2015-12-30 주식회사 애니웨어 Bactericidal and pesticidal agent composition containing plant extract as active ingredient
CN105941504A (en) * 2016-05-25 2016-09-21 柳州市能兴科技有限公司 Botanical source fungicide capable of preventing and treating strawberry root rot
CN106342939A (en) * 2016-08-31 2017-01-25 江苏星源生物科技有限公司 Biological agent for preventing and treating peanut root rot and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2958500B1 (en) * 2010-04-07 2013-02-08 Jd Invest PREPARATION INSECTICIDE, INSECTIFUGE, OVICIDE, LARVICIDE, NYMPHICIDE

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015199342A1 (en) * 2014-06-26 2015-12-30 주식회사 애니웨어 Bactericidal and pesticidal agent composition containing plant extract as active ingredient
CN105941504A (en) * 2016-05-25 2016-09-21 柳州市能兴科技有限公司 Botanical source fungicide capable of preventing and treating strawberry root rot
CN106342939A (en) * 2016-08-31 2017-01-25 江苏星源生物科技有限公司 Biological agent for preventing and treating peanut root rot and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Efficasy Of Plant Essential Oils In Controlling Damping–Off And Root Rots Diseases Of Peanut As Fungicides Alternative;Mahmoud, E.Y.et al;《Journal of Applied Sciences Research》;20131231;第9卷(第3期);第1612-1622页 *

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