CN115191450B - Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and application method thereof - Google Patents

Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and application method thereof Download PDF

Info

Publication number
CN115191450B
CN115191450B CN202210969094.8A CN202210969094A CN115191450B CN 115191450 B CN115191450 B CN 115191450B CN 202210969094 A CN202210969094 A CN 202210969094A CN 115191450 B CN115191450 B CN 115191450B
Authority
CN
China
Prior art keywords
rheum officinale
chinese herbal
preparation
herbal medicine
tomato
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210969094.8A
Other languages
Chinese (zh)
Other versions
CN115191450A (en
Inventor
包志龙
马方放
刘根忠
马明轩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Agricultural University
Original Assignee
Shandong Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Agricultural University filed Critical Shandong Agricultural University
Priority to CN202210969094.8A priority Critical patent/CN115191450B/en
Publication of CN115191450A publication Critical patent/CN115191450A/en
Application granted granted Critical
Publication of CN115191450B publication Critical patent/CN115191450B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/30—Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pest Control & Pesticides (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Insects & Arthropods (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a Chinese herbal medicine rheum officinale preparation for promoting tomato plant growth and a use method thereof, wherein the Chinese herbal medicine preparation comprises the following steps: weighing a proper amount of rheum officinale, placing the rheum officinale into a glass container, adding 10 times of water of the volume of the rheum officinale serving as a Chinese herbal medicine into the container, soaking for 1 hour, placing the rheum officinale into a water bath kettle at 100 ℃ for heating for 30 minutes, filtering and collecting liquid medicine by using a funnel and filter paper, adding the same amount of water into filter residues, heating the filter residues for 30 minutes in the water bath kettle at 100 ℃, filtering and collecting the liquid medicine by using the funnel and the filter paper, combining the liquid medicine twice, diluting to the required concentration until the concentration of the rheum officinale preparation is 6g/L, and using the rheum officinale serving as the Chinese herbal medicine preparation to partially replace chemical pesticides and fertilizers such as pesticides and bactericides in the conventional agriculture, so that the rheum officinale is environment-friendly, harmless to people and livestock, free of heavy metal and pesticide residues and safer in products.

Description

Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and application method thereof
Technical Field
The invention relates to the technical field of plant biostimulants, in particular to a Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and a use method thereof.
Background
Tomato, i.e. tomato, persimmon, belongs to a annual or perennial herb of the order of the tubular flower, the family of the Solanaceae, the genus Lycopersicon. The tomato fruits are rich in vitamins, dietary fibers, organic acids, carotenoids and other nutrient substances, are deeply favored by people, are vegetable crops with the widest cultivation and the largest consumption in the world, have continuously increased demand, realize the continuous targets of high yield, stable yield and high quality of tomatoes, and realize the great use of fertilizers and pesticides in a great way for improving the yield and quality of tomatoes.
In recent years, to obtain maximum yields, leading to a large and blind use of fertilizers and pesticides in agricultural production, a series of problems are posed: the ecological balance of farmland is damaged, the environment is seriously polluted, the health of people and livestock is endangered, and the plants are regulated by biological stimulation. The biological hormone is a novel product appearing in the agricultural market, mainly comprises seaweed extract, humic acid, chitin and derivatives thereof, microbial agents and the like, and has the effects of promoting crop growth, improving nutrient absorption and utilization efficiency and the like. At present, the biological stimulation hormone is gradually and widely applied to horticultural crops and grain crops, achieves the effects of increasing yield and income, provides assistance for the 'chemical pesticide fertilizer application reduction and synergy', and plays an important role in the rapid development of sustainable agricultural practice. Therefore, the development and application of the novel biological stimulation hormone have considerable prospect in the medical fertilizer market.
Chinese herbal medicine resources in China are rich, and recent researches show that secondary metabolic substances in the Chinese herbal medicine have various regulation and control effects on plant growth. The Chinese herbal medicine has the advantages of green, no pollution and environmental friendliness, and the natural Chinese herbal medicine is used as the raw material to prepare the novel plant biological hormone with the growth regulating activity, so that the novel plant biological hormone has an important role in the field of plant growth regulation. The Chinese herbal medicine extract is different from the conventional growth regulator, is environment-friendly and harmless to human and livestock. The rheum officinale is taken as a traditional Chinese herbal medicine in China, belongs to Labiatae plants, has wide pharmacological activity, has various pharmacological effects including anti-tumor, anti-oxidation, anti-inflammatory, antimicrobial, anti-aging and the like, is low in price and rich in resources, and is an excellent resource for developing novel biological stimulation hormone. Therefore, the Chinese herbal medicine with moderate price is selected, the Chinese herbal medicine is prepared into an environment-friendly Chinese herbal medicine extract, the plant activity regulating effect is utilized, the Chinese herbal medicine extract is directly applied to crops, and a new way is opened up for the development of natural green pollution-free biological hormone.
Therefore, we propose a Chinese herbal medicine rhubarb preparation for promoting tomato plant growth and a use method thereof.
Disclosure of Invention
The invention mainly aims to provide a Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and a using method thereof, which can effectively solve the problems in the background technology.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
in a first aspect, a Chinese herbal medicine preparation for promoting growth of tomato plants comprises rheum officinale preparation as a component.
In a second aspect, the present invention also provides a method for preparing a Chinese herbal medicine preparation for promoting growth of tomato plants, comprising the following steps:
(1) Weighing a proper amount of rheum officinale, placing the rheum officinale into a glass container, adding water with the volume 10 times that of the Chinese herbal medicines into the container, and soaking for 1 hour;
(2) Heating in a water bath at 100deg.C for 30 min, filtering with a funnel and filter paper to collect medicinal liquid, adding equal amount of water into the residue, and heating in a water bath at 100deg.C for 30 min;
(3) Filtering with funnel and filter paper, collecting medicinal liquid, mixing the medicinal liquid, and diluting to desired concentration until the concentration of radix et rhizoma Rhei preparation is 6g/L.
Preferably, the water used in step 1 and step 2 is distilled water.
In a third aspect, the present invention provides a method of using a herbal formulation for promoting the growth of tomato plants comprising: the acting crop is tomato, and the acting part is root.
Compared with the prior art, the invention has the following beneficial effects: based on natural Chinese herbal medicine rhubarb preparation as biological hormone, tomato plants are treated by adopting a root irrigation mode of the Chinese herbal medicine rhubarb preparation, so that the tomato stems, root system size, photosynthetic pigment content, net photosynthetic rate and transpiration rate are increased, biomass accumulation is promoted, and strong seedling index is improved. Therefore, the Chinese herbal medicine rheum officinale preparation is used as a natural green pollution-free novel biological hormone which has the function of promoting the growth of tomatoes, does not cause the residues of heavy metals and pesticides of tomato plants, and has higher safety; the Chinese herbal medicine rheum officinale preparation is used for partially replacing chemical pesticides and fertilizers such as pesticides and bactericides in conventional agriculture, is environment-friendly, harmless to human and livestock, free of heavy metal and pesticide residues, and safer in product.
Drawings
FIG. 1 is a schematic diagram showing the effect of different treatment modes of the rhubarb preparation provided by the invention on the growth of tomato plant stalks; wherein, figure a is the overall plant growth phenotype; panel B is plant height; panel C shows the plant stem thickness.
FIG. 2 is a schematic diagram showing the effect of different treatment modes of the rhubarb preparation on the area of tomato leaves; wherein, panel a is plant small She Biaoxing; panel B is the leaflet area.
FIG. 3 is a schematic diagram showing the effect of different treatment modes of the rhubarb preparation provided by the invention on the photosynthetic pigment of tomato leaf; wherein, the graph A is the chlorophyll a content of the leaf; FIG. B shows chlorophyll B content of leaf blades; panel C shows total chlorophyll content of leaf blades; panel D shows leaf carotenoid content.
FIG. 4 is a schematic diagram showing the influence of different treatment modes of the rhubarb preparation provided by the invention on the gas exchange parameters of tomato leaves; wherein, graph a is leaf net photosynthetic rate; graph B is leaf transpiration rate; panel C is the concentration of carbon dioxide in the leaf cells.
FIG. 5 is a schematic diagram showing the influence of different treatment modes of the rhubarb preparation on the root system size of tomatoes; wherein, figure a is tomato root phenotype; panel B is root mean diameter; panel C is the root volume.
FIG. 6 is a schematic diagram showing the effect of different treatment modes of the rhubarb preparation provided by the invention on tomato plant biomass; wherein, panel a is the biomass of the upper part of the tomato plant; panel B is biomass of the subsurface portion of a tomato plant.
FIG. 7 is a schematic diagram showing the influence of different treatment modes of the rhubarb preparation on root-cap ratio and strong seedling index; wherein, the graph A is the plant root-cap ratio; panel B is the plant strengthening index.
FIG. 8 is a heat map of the growth-related index of the rhubarb preparation provided by the invention under different treatment modes.
Wherein: PL represents plant height; SD represents plant stalk diameter; LA represents the leaflet area; ca represents leaf chlorophyll a; cb represents leaf chlorophyll b; chl represents leaf total chlorophyll; caro represents leaf carotenoid; PR represents the net photosynthetic rate of the leaf; e represents the leaf transpiration rate; ci represents the intercellular carbon dioxide concentration of the leaf; ARD represents the root mean diameter; RV represents root volume; AF represents the fresh weight of the aerial parts of tomato plants; AD represents dry weight of aerial parts of tomato plants; UF represents the fresh weight of the underground part of the tomato plant; UD represents the dry weight of the underground part of the tomato plant; RS represents the ratio of root to crown of tomato plants; SSI represents the tomato plant strong seedling index. CK represents leaf spraying water and root irrigation water; s represents leaf spraying of rheum officinale preparation and root irrigation water; r represents leaf spraying water and root irrigation rheum officinale preparation; SR means leaf spray rhubarb preparation and root irrigation rhubarb preparation.
Detailed Description
The invention is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Examples
A Chinese herbal medicine preparation for promoting tomato plant growth comprises rheum officinale preparation.
The Chinese herbal medicine preparation in the embodiment is obtained by the following steps:
(1) Weighing a proper amount of rheum officinale, placing the rheum officinale into a glass container, adding water with the volume 10 times that of the Chinese herbal medicines into the container, and soaking for 1 hour;
(2) Heating in a water bath at 100deg.C for 30 min, filtering with a funnel and filter paper to collect medicinal liquid, adding equal amount of water into the residue, and heating in a water bath at 100deg.C for 30 min;
(3) Filtering with funnel and filter paper, collecting medicinal liquid, mixing the medicinal liquid, and diluting to desired concentration until the concentration of radix et rhizoma Rhei preparation is 6g/L.
The application method of the Chinese herbal medicine preparation in the embodiment is as follows:
in the period of four leaves and one heart of the tomato, the tomato seedlings are treated in a spraying and root irrigation mode, and the treatment is carried out once every three days, and five times of treatment are carried out;
the acting crop is tomato, and the acting part is root.
Test example: the tomato variety tested was "Ailsa Craig".
Test grouping: accelerating germination of tomato seeds in a 28 ℃ incubator, sowing the tomato seeds in a plug tray filled with vermiculite after the seeds are exposed to white, and watering 1/2Hogland nutrient solution every day. When tomatoes grow to four leaves and one center, selecting seedlings with consistent growth vigor, dividing the seedlings into two groups with similar growth vigor, and transplanting the seedlings to the soil8 of nutrient matrix (turf: perlite: vermiculite = 3:1:1) ╳ In a 9cm small pot, the temperature is controlled at about 25 ℃, the humidity is about 65%, the light is irradiated for 16 hours, and the darkness is carried out for 8 hours. Treating in 4 groups, wherein the first group is blade spraying water and root irrigation water, and the CK is used for representing; the second group is to spray rhubarb preparation and root irrigation water for the leaves, which is denoted by S; the third group is to spray water on the leaves and irrigate rheum officinale preparation on the roots, and the third group is represented by R; the fourth group is leaf spray rhubarb preparation and root irrigation rhubarb preparation, which are expressed by SR, and treated once every three days for 5 times.
The test mode is as follows: a completely randomized block design, 8 biological replicates were set.
Experimental results: referring to fig. 1 and 2, compared with the control, the areas of the stems and the leaves of the plants are obviously increased by 7.50 percent and 14.83 percent by R treatment, the heights of the plants treated by R are increased by 2.60 percent by CK, but the differences are not obvious; referring to fig. 3, the contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoid in the R-treated leaves were improved by 9.74%, 22.39%, 9.66% and 14.01%, respectively, compared to the control, and the differences were significant; referring to fig. 4, the net photosynthetic rate and transpiration rate of the leaves treated with R were significantly higher than those of CK by 16.48% and 48.21%, respectively, the intercellular carbon dioxide concentration was lower with R by 16.97% than CK, and the difference was significant; referring to fig. 5, the average root diameter and root volume of the R treated group increased by 13.72% and 23.13%, respectively, with a significant difference compared to CK; referring to fig. 6, the fresh weight and dry weight of the above-ground part under R treatment are improved by 6.83% and 17.26% respectively compared with CK, but only the dry weight is significantly different, the fresh weight and dry weight of the below-ground part under R treatment are most significantly different compared with CK, and are improved by 39.14% and 17.94% respectively, and the difference is significant; referring to fig. 7, the root-to-crown ratio and strong seedling index of tomato are highest under R treatment and are 47.41% and 18.78% higher than CK treatment respectively, and the difference is obvious; referring to fig. 8, the physiological index related to plant growth under the R treatment mode is generally better than that of the control group, which indicates that the R treatment mode is more beneficial to cultivating strong tomato seedlings.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (3)

1. The application of the Chinese herbal medicine preparation in promoting the growth of tomato plants is characterized in that the Chinese herbal medicine preparation comprises a rheum officinale preparation; the preparation method of the Chinese herbal medicine rheum officinale preparation comprises the following operation steps:
(1) Weighing a proper amount of rheum officinale, placing the rheum officinale into a glass container, adding water which is 10 times of the volume of the rheum officinale serving as a Chinese herbal medicine into the container, and soaking for 1 hour;
(2) Heating in a water bath at 100deg.C for 30 min, filtering with a funnel and filter paper to collect medicinal liquid, adding equal amount of water into the residue, and heating in a water bath at 100deg.C for 30 min;
(3) Filtering with funnel and filter paper, collecting medicinal liquid, mixing the medicinal liquid, and diluting to desired concentration until the concentration of radix et rhizoma Rhei preparation is 6g/L.
2. Use of a herbal formulation according to claim 1 for promoting the growth of tomato plants, wherein: in the period of four leaves and one heart of the tomato, the tomato seedlings are treated by spraying and root irrigation, and the treatment is carried out once every three days, and the total treatment is carried out five times.
3. The use of a herbal formulation for promoting the growth of tomato plants as claimed in claim 1, wherein: the action parts are the blade and the root.
CN202210969094.8A 2022-08-12 2022-08-12 Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and application method thereof Active CN115191450B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210969094.8A CN115191450B (en) 2022-08-12 2022-08-12 Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and application method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210969094.8A CN115191450B (en) 2022-08-12 2022-08-12 Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and application method thereof

Publications (2)

Publication Number Publication Date
CN115191450A CN115191450A (en) 2022-10-18
CN115191450B true CN115191450B (en) 2023-05-16

Family

ID=83586950

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210969094.8A Active CN115191450B (en) 2022-08-12 2022-08-12 Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and application method thereof

Country Status (1)

Country Link
CN (1) CN115191450B (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1268214C (en) * 2002-06-20 2006-08-09 河北农业大学植物保护学院 Germicide containing rhubarb extract and its composition
CN105211120B (en) * 2015-10-14 2017-11-28 帕潘纳(北京)科技有限公司 The antibacterial synergistic composition of plant and its preparation and application
KR102021550B1 (en) * 2017-11-30 2019-09-16 (주)현농 control agent of plant viruses using Rhei Rhizoma
US20190343127A1 (en) * 2018-05-08 2019-11-14 Inveran Corp Spa Agricultural composition for increasing crop yields

Also Published As

Publication number Publication date
CN115191450A (en) 2022-10-18

Similar Documents

Publication Publication Date Title
CN105052655B (en) Oil tea implantation methods
CN106797861A (en) A kind of oil tea implantation methods
CN105145253A (en) Method for planting organic passion fruit trees
CN105875331A (en) Sugar orange and sweet potato interplanting method
CN107624489A (en) A kind of implantation methods of selenium-rich lichee
CN107455212A (en) A kind of implantation methods of Chinese pear
CN108739182B (en) Standardized planting method for bletilla striata and blueberry intercropping
CN110972864A (en) Selenium-rich cultivation method for Bigheng white atractylodes rhizome
CN106577123A (en) Planting method of randia cochinchinensis
CN100333631C (en) Culture of wild barcken
CN107135889A (en) A kind of grape planting process of green ecological
KR100555077B1 (en) Ferment solution of plants and method for fertilizer application or killing insects using the solution
CN103070070A (en) Cultivation method of seedless roxburgh roses
CN109076849A (en) A kind of implantation methods of Wampee tree
CN109089755B (en) Planting method of broccoli sprouting vegetable
CN115191450B (en) Chinese herbal medicine rheum officinale preparation for promoting growth of tomato plants and application method thereof
CN109275479A (en) A kind of implantation methods of blood orange
CN109220478A (en) A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf
CN104221700A (en) Novel perilla frutescens planting method
CN108812165A (en) A kind of high-yield planting method of dendrobium nobile
CN109937814A (en) A kind of implantation methods of passion fruit
CN115281219B (en) Chinese herbal medicine baical skullcap root preparation for promoting growth of tomato plants and application method thereof
CN107278564A (en) Organic bright red mandarin orange and the method for pea spacing sleeve cropping
CN112425436A (en) Planting method for intercropping blueberry and asparagus cochinchinensis
CN108782199B (en) Epiphytic planting method for dendrobium

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant